                         SEQUENCE LISTING

<110>  The Ohio State University
       Yang, Shang-Tian
       Yu, Mingrui
 
<120>  METABOLIC ENGINEERING OF CLOSTRIDIUM TYROBUTYRICUM FOR BUTANOL 
       PRODUCTION

<130>  OSU1159-301

<150>  US PROVISIONAL NO. 61/389,060
<151>  2010-10-01

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  DNA
<213>  Clostridium acetobutylicum, ATCC 824

<400>  1
atggatcctt ttataaagga gtgtatataa atgaaag                                37


<210>  2
<211>  36
<212>  DNA
<213>  Clostridium acetobutylicum, ATCC 824

<400>  2
ttggcgccat aatgaagcaa agactatttt acattc                                 36


<210>  3
<211>  56
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  3
atggatccat aaatatttag gaggaatagt catgaaagtt acaaatcaaa aagaac           56


<210>  4
<211>  38
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  4
tctaccgcgg ataatgaagc aaagactatt ttacattc                               38


<210>  5
<211>  31
<212>  DNA
<213>  Clostridium tyrobutyricum

<400>  5
agctaagctt ctgaatattc agcgaaaata g                                      31


<210>  6
<211>  51
<212>  DNA
<213>  Clostridium tyrobutyricum

<400>  6
tctaccgcgg acgtcggatc caaatttaaa ttgattacaa acctttttac c                51


<210>  7
<211>  7000
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  7
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcg      240

ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct      300

attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg      360

gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgagc ttaatcgcat      420

ttcatagatt gacctcccaa taactacgtg gtgttattgg gaggtcaatc tatttcattt      480

gcctcttgct caaagttccc aaattcgagt aagaggtatt tttgtttttg gtcgtcgcct      540

ctcattagta gttcagggtt taacattaat actccagttt ttctttttat aatatttcct      600

tcttctaaga ttttaagtgt tgttattact gtttgtagac ttgttcctgt agcttttgct      660

atttctcttg ttgtagctat cattgtattg ttacttaagt ggacattatc taggatatag      720

ttaacgattt taagtttttt tccgccaatc atatctaaca tacttattaa ttgcactata      780

tatgccttta cgaagttacc agacgtttgt ttacggtata acttgtctac ctctatgact      840

tctccacttt cttcgtctat gagcctctga gagcctttat agactgttcc atatctttct      900

ttcatctttt tctcactcct tattttaaac tattctaact atatcataac tgttctaaaa      960

aaaaaagaac atttgttaaa agaaattaga acaaaatgag tgaaaaatta gaacaaacaa     1020

attccttata aaccttatca tctcaaccta tattaagatt ttacctagtt gaatcttctt     1080

ttctatataa agcgtcggag catatcaggg ggttatctaa cgtaaatgct acccttcggc     1140

tcgctttcgc tcggcattga cgtcagatac tgcaccccct gaacccccat gctccaacag     1200

caaaaaggaa actttttgct gcttttccga cgcttattcg cttcgctcat atttatatag     1260

aaaagaagtg aatgcgcaaa agacataatc gattcacaaa aaataggtac acgaaaaaca     1320

agttaaggga tgcagtttat gcatccctta acttacttat taaataattt atagctattg     1380

aaaagagata agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt     1440

aaggaattgt taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc     1500

ataacgaaat aattatactt ctgtttatct ttgtgtgata ttcttgattt ttttctattt     1560

aatctgataa gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata     1620

cttaatatag aaatatcaac ttctgccatt aaaaataatg ccaatgagcg ttttgtattt     1680

aataatcttt tagcaaaccc gtattccacg attaaataaa tctcatcagc tatactatca     1740

aaaacaattt tgcgtattat atccgtactt atgttataag gtatattacc aaatatttta     1800

taggattggt ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta     1860

tcgtgatcaa caagtttatt ttctgtagtt ttgcataatt tatggtctat ttcaatggca     1920

gttacgaaat tacacctctg tactaattca agggtaaaat gcccttttcc tgagccgatt     1980

tcaaagatat tatcatgttc atttaatctt atatttgtca ttattttatc tatattatgt     2040

tttgaagtaa taaagttttg actgtgtttt atatttttct cgttcattat aaccctcttt     2100

attttttcct ccttataaaa ttagtataat tatagcacga gctctgataa atatgaacat     2160

gatgagtgat cgttaaattt atactgcaat ctgatgcgat tattgaataa aagatatgag     2220

agatttatct agtttctttt tttacaagaa aaaagaaagt tcttaaaggt tttatacttt     2280

tggtcgtaga gcacacggtt taacgactta attacgaagt aaataagtct agtgtgttag     2340

actttaatgt ttttttaagg cattagtgca tttaagcgtc agagcatggc tttatgccga     2400

gaaaactatt ggttggaatg gcgtgtgtgt tagccaaagc tcctgcaggt cgactgtgga     2460

tggagttaag tcagcagaaa gtataatgag aaaatataaa atataaataa ttttctaaaa     2520

aacttaactt catgtgaaaa gtttgttaaa atataaatga gcacgttaat catttaacat     2580

agataattgg atccagaatt taaaaggagg gattaaaatg aactctaaaa taattagatt     2640

tgaaaattta aggtcattct ttaaagatgg gatgacaatt atgattggag gttttttaaa     2700

ctgtggcact ccaaccaaat taattgattt tttagttaat ttaaatataa agaatttaac     2760

gattataagt aatgatacat gttatcctaa tacaggtatt ggtaagttaa tatcaaataa     2820

tcaagtaaaa aagcttattg cttcatatat aggcagcaac ccagatactg gcaaaaaact     2880

ttttaataat gaacttgaag tagagctctc tccccaagga actctagtgg aaagaatacg     2940

tgcaggcgga tctggcttag gtggtgtact aactaaaaca ggtttaggaa ctttgattga     3000

aaaaggaaag aaaaaaatat ctataaatgg aacggaatat ttgttagagc tacctcttac     3060

agccgatgta gcattaatta aaggtagtat tgtagatgag gccggaaaca ccttctataa     3120

aggtactact aaaaacttta atccctatat ggcaatggca gctaaaaccg taatagttga     3180

agctgaaaat ttagttagct gtgaaaaact agaaaaggaa aaagcaatga cccccggagt     3240

tcttataaat tatatagtaa aggagcctgc ataaaatgat taatgataaa aacctagcga     3300

aagaaataat agccaaaaga gttgcaagag aattaaaaaa tggtcaactt gtaaacttag     3360

gtgtaggtct tcctaccatg gttgcagatt atataccaaa aaatttcaaa attactttcc     3420

aatcagaaaa cggaatagtt ggaatgggcg ctagtcctaa aataaatgag gcagataaag     3480

atgtagtaaa tgcaggagga gactatacaa cagtacttcc tgacggcaca tttttcgata     3540

gctcagtttc gttttcacta atccgtggtg gtcacgtaga tgttactgtt ttaggggctc     3600

tccaggtaga tgaaaagggt aatatagcca attggattgt tcctggaaaa atgctctctg     3660

gtatgggtgg agctatggat ttagtaaatg gagctaagaa agtaataatt gcaatgagac     3720

atacaaataa aggtcaacct aaaattttaa aaaaatgtac acttcccctc acggcaaagt     3780

ctcaagcaaa tctaattgta acagaacttg gagtaattga ggttattaat gatggtttac     3840

ttctcactga aattaataaa aacacaacca ttgatgaaat aaggtcttta actgctgcag     3900

atttactcat atccaatgaa cttagaccca tggctgttta gaaagaattc ttgatatcag     3960

gaaggtgact tttatgttaa aggatgaagt aattaaacaa attagcacgc cattaacttc     4020

gcctgcattt cctagaggac cctataaatt tcataatcgt gagtatttta acattgtata     4080

tcgtacagat atggatgctc ttcgtaaagt tgtgccagag cctttagaaa ttgatgagcc     4140

cttagtcagg tttgaaatta tggcaatgca tgatacgagt ggacttggtt gttatacaga     4200

aagcggacag gctattcccg taagctgtaa tggagttaag ggagattatc ttcatatgat     4260

gtatttagat aatgagcctg caattgcagt aggaagggaa ttaagtgcat atcctaaaaa     4320

gctcgggtat ccaaagcttt ttgtggattc agatacttta gtaggaactt tagactatgg     4380

aaaacttaga gttgcgacag ctacaatggg gtacaaacat aaagccttag atgctaatga     4440

agcaaaggat caaatttgtc gccctaatta tatgttgaaa ataataccca attatgatgg     4500

aagccctagg atatgtgagc ttataaatgc gaaaatcaca gatgttaccg tacatgaagc     4560

ttggacagga ccaactcgac tgcagttatt tgatcacgct atggcgccac ttaatgattt     4620

gccagtaaaa gagattgttt ctagctctca cattcttgca gatataatat tgcctagagc     4680

tgaagttata tatgattatc ttaagtaata aaaataagag ttaccttaaa tggtaactct     4740

tattttttta atgtcgactc atagaattcg taatcatggt catagctgtt tcctgtgtga     4800

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc     4860

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     4920

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     4980

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     5040

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     5100

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     5160

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     5220

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     5280

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     5340

gcctttctcc cttcgggaag cgtggcgctt tctcaaagct cacgctgtag gtatctcagt     5400

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     5460

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     5520

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     5580

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     5640

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     5700

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     5760

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     5820

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     5880

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     5940

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     6000

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     6060

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     6120

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     6180

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     6240

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     6300

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     6360

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     6420

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     6480

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     6540

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     6600

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     6660

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     6720

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     6780

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     6840

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     6900

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     6960

ttaacctata aaaataggcg tatcacgagg ccctttcgtc                           7000


<210>  8
<211>  11845
<212>  DNA
<213>  Clostridium acetobutylicum

<400>  8
agcttataat tatccttacg tgacggttaa gtgcgcccag atagggtgtt aagtcaagta       60

gtttaaggta ctactctgta agataacaca gaaaacagcc aacctaaccg aaaagcgaaa      120

gctgatacgg gaacagagca cggttggaaa gcgatgagtt acctaaagac aatcgggtac      180

gactgagtcg caatgttaat cagatataag gtataagttg tgtttactga acgcaagttt      240

ctaatttcgg tttcacgtcg atagaggaaa gtgtctgaaa cctctagtac aaagaaaggt      300

aagttacgtt aaccgactta tctgttatca ccacatttgt acaatctgta ggagaaccta      360

tgggaacgaa acgaaagcga tgccgagaat ctgaatttac caagacttaa cactaactgg      420

ggatacccta aacaagaatg cctaatagaa aggaggaaaa aggctatagc actagagctt      480

gaaaatcttg caagggtacg gagtactcgt agtagtctga gaagggtaac gccctttaca      540

tggcaaaggg gtacagttat tgtgtactaa aattaaaaat tgattaggga ggaaaacctc      600

aaaatgaaac caacaatggc aattttagaa agaatcagta aaaattcaca agaaaatata      660

gacgaagttt ttacaagact ttatcgttat cttttacgtc cagatattta ttacgtggcg      720

acgcgtgcga ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag      780

acaatacttg ctcataagta acggtactta aattgtttac tttggcgtgt ttcattgctt      840

gatgaaactg atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa      900

agtacgtata tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta      960

ttaagacact gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt     1020

gctgaatcga gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac     1080

gcttgtagaa tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca     1140

cttttttaat aatttgtgtg cttaaatggt aaggaatact cccaacaatt ttatacctct     1200

gtttgttagg gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgagtattca     1260

gttttaattt ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct     1320

gtttacttat tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa     1380

cggtatcggt ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg     1440

ttaaaaagtt ttgagaatat tttatatttt tgttcatacc agcaccagaa gcaccagcat     1500

ctcttgggtt aattgaggcc tgagtataag gtgacttata cttgtaatct atctaaacgg     1560

ggaacctctc tagtagacaa tcccgtgcta aattgtagga ctgcccttta ataaatactt     1620

ctatatttaa agaggtattt atgaaaagcg gaatttatca gattaaaaat actttctcta     1680

gagaaaattt cgtctggatt agttacttat cgtgtaaaat ctgataaatg gaattggttc     1740

tacataaatg cctaacgact atccctttgg ggagtagggt caagtgactc gaaacgatag     1800

acaacttgct ttaacaagtt ggagatatag tctgctctgc atggtgacat gcagctggat     1860

ataattccgg ggtaagatta acgaccttat ctgaacataa tgccatatga atccctccta     1920

atttatacgt tttctctaac aacttaatta tacccactat tattattttt atcaatataa     1980

cgcgttggga aatggcaatg atagcgaaac aacgtaaaac tcttgttgta tgctttcatt     2040

gtcatcgtca cgtgattcat aaacacaagt gaatgtcgac agtgaatttt tacgaacgaa     2100

caataacaga gccgtatact ccgagagggg tacgtacggt tcccgaagag ggtggtgcaa     2160

accagtcaca gtaatgtgaa caaggcggta cctccctact tcaccatatc attttctgca     2220

gccccctaga aataattttg tttaacttta agaaggagat atacatatat ggctagatcg     2280

tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc     2340

aatttctatg cactcgtagt agtctgagaa gggtaacgcc ctttacatgg caaaggggta     2400

cagttattgt gtactaaaat taaaaattga ttagggagga aaacctcaaa atgaaaccaa     2460

caatggcaat tttagaaaga atcagtaaaa attcacaaga aaatatagac gaagttttta     2520

caagacttta tcgttatctt ttacgtccag atatttatta cgtggcgtat caaaatttat     2580

attccaataa aggagcttcc acaaaaggaa tattagatga tacagcggat ggctttagtg     2640

aagaaaaaat aaaaaagatt attcaatctt taaaagacgg aacttactat cctcaacctg     2700

tacgaagaat gtatattgca aaaaagaatt ctaaaaagat gagaccttta ggaattccaa     2760

ctttcacaga taaattgatc caagaagctg tgagaataat tcttgaatct atctatgaac     2820

cggtattcga agatgtgtct cacggtttta gacctcaacg aagctgtcac acagctttga     2880

aaacaatcaa aagagagttt ggcggcgcaa gatggtttgt ggagggagat ataaaaggct     2940

gcttcgataa tatagaccac gttacactca ttggactcat caatcttaaa atcaaagata     3000

tgaaaatgag ccaattgatt tataaatttc taaaagcagg ttatctggaa aactggcagt     3060

atcacaaaac ttacagcgga acacctcaag gtggaattct atctcctctt ttggccaaca     3120

tctatcttca tgaattggat aagtttgttt tacaactcaa aatgaagttt gaccgagaaa     3180

gtccagaaag aataacacct gaatatcggg agctccacaa tgagataaaa agaatttctc     3240

accgtctcaa gaagttggag ggtgaagaaa aagctaaagt tcttttagaa tatcaagaaa     3300

aacgtaaaag attacccaca ctcccctgta cctcacagac aaataaagta ttgaaatacg     3360

tccggtatgc ggacgacttc attatctctg ttaaaggaag caaagaggac tgtcaatgga     3420

taaaagaaca attaaaactt tttattcata acaagctaaa aatggaattg agtgaagaaa     3480

aaacactcat cacacatagc agtcaacccg ctcgttttct gggatatgat atacgagtaa     3540

ggagatctgg aacgataaaa cgatctggta aagtcaaaaa gagaacactc aatgggagtg     3600

tagaactcct tattcctctt caagacaaaa ttcgtcaatt tatttttgac aagaaaatag     3660

ctatccaaaa gaaagatagc tcatggtttc cagttcacag gaaatatctt attcgttcaa     3720

cagacttaga aatcatcaca atttataatt ctgaactccg cgggatttgt aattactacg     3780

gtctagcaag taattttaac cagctcaatt attttgctta tcttatggaa tacagctgtc     3840

taaaaacgat agcctccaaa cataagggaa cactttcaaa aaccatttcc atgtttaaag     3900

atggaagtgg ttcgtggggg atcccgtatg agataaagca aggtaagcag cgccgttatt     3960

ttgcaaattt tagtgaatgt aaatcccctt atcaatttac ggatgagata agtcaagctc     4020

ctgtattgta tggctatgcc cggaatactc ttgaaaacag gttaaaagct aaatgttgtg     4080

aattatgtgg gacgtctgat gaaaatactt cctatgaaat tcaccatgtc aataaggtca     4140

aaaatcttaa aggcaaagaa aaatgggaaa tggcaatgat agcgaaacaa cgtaaaactc     4200

ttgttgtatg ctttcattgt catcgtcacg tgattcataa acacaagtga atgtcgagca     4260

cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta     4320

cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcgccaag     4380

ctcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat     4440

aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa     4500

cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc     4560

cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc     4620

catcctgacg gatggccttt ttgcgtttct acaaactctt cctgtcgtca tatctacaag     4680

ccatcccccc acagatacgg taaactagcc tcgtttttgc atcaggaaag cagaacgcca     4740

tgagcggcct catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct     4800

tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc     4860

aactcgtagg acaggtgcca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga     4920

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     4980

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     5040

atggcgctag cgaagagatg cagcagccat tatttttttg aacaattgac aattcatttc     5100

ttatttttta ttaagtgata gtcaaaaggc ataacagtgc tgaatagaaa gaaatttaca     5160

gaaaagaaaa ttatagaatt tagtatgatt aattatactc atttatgaat gtttaattga     5220

atacaaaaaa aaatacttgt tatgtattca attacgggtt aaaatataga caagttgaaa     5280

aatttaataa aaaaataagt cctcagctct tatatattaa gctaccaact tagtatataa     5340

gccaaaactt aaatgtgcta ccaacacatc aagccgttag agaactctat ctatagcaat     5400

atttcaaatg taccgacata caagagaaac attaactata tatattcaat ttatgagatt     5460

atcttaacag atataaatgt aaattgcaat aagtaagatt tagaagttta tagcctttgt     5520

gtattggaag cagtacgcaa aggctttttt atttgataaa aattagaagt atatttattt     5580

tttcataatt aatttatgaa aatgaaaggg ggtgagcaaa gtgacagagg aaagcagtat     5640

cttatcaaat aacaaggtat tagcaatatc attattgact ttagcagtaa acattatgac     5700

ttttatagtg cttgtagcta agtagtacga aagggggagc tttaaaaagc tccttggaat     5760

acatagaatt cataaattaa tttatgaaaa gaagggcgta tatgaaaact tgtaaaaatt     5820

gcaaagagtt tattaaagat actgaaatat gcaaaataca ttcgttgatg attcatgata     5880

aaacagtagc aacctattgc agtaaataca atgagtcaag atgtttacat aaagggaaag     5940

tccaatgtat taattgttca aagatgaacc gatatggatg gtgtgccata aaaatgagat     6000

gttttacaga ggaagaacag aaaaaagaac gtacatgcat taaatattat gcaaggagct     6060

ttaaaaaagc tcatgtaaag aagagtaaaa agaaaaaata atttatttat taatttaata     6120

ttgagagtgc cgacacagta tgcactaaaa aatatatctg tggtgtagtg agccgataca     6180

aaaggatagt cactcgcatt ttcataatac atcttatgtt atgattatgt gtcggtggga     6240

cttcacgacg aaaacccaca ataaaaaaag agttcggggt agggttaagc atagttgagg     6300

caactaaaca atcaagctag gatatgcagt agcagaccgt aaggtcgttg tttaggtgtg     6360

ttgtaataca tacgctatta agatgtaaaa atacggatac caatgaaggg aaaagtataa     6420

tttttggatg tagtttgttt gttcatctat gggcaaacta cgtccaaagc cgtttccaaa     6480

tctgctaaaa agtatatcct ttctaaaatc aaagtcaagt atgaaatcat aaataaagtt     6540

taattttgaa gttattatga tattatgttt ttctattaaa ataaattaag tatatagaat     6600

agtttaataa tagtatatac ttaatgtgat aagtgtctga cagctgaccg gtctaaagag     6660

gtccctagcg cctacgggga atttgtatcg ataaggggta caaattccca ctaagcgctc     6720

ggcggggatc gatcccgggt acgtacccgg cagtttttct ttttcggcaa gtgttcaaga     6780

agttattaag tcgggagtgc agtcgaagtg ggcaagttga aaaattcaca aaaatgtggt     6840

ataatatctt tgttcattag agcgataaac ttgaatttga gagggaactt agatggtatt     6900

tgaaaaaatt gataaaaata gttggaacag aaaagagtat tttgaccact actttgcaag     6960

tgtaccttgt acctacagca tgaccgttaa agtggatatc acacaaataa aggaaaaggg     7020

aatgaaacta tatcctgcaa tgctttatta tattgcaatg attgtaaacc gccattcaga     7080

gtttaggacg gcaatcaatc aagatggtga attggggata tatgatgaga tgataccaag     7140

ctatacaata tttcacaatg atactgaaac attttccagc ctttggactg agtgtaagtc     7200

tgactttaaa tcatttttag cagattatga aagtgatacg caacggtatg gaaacaatca     7260

tagaatggaa ggaaagccaa atgctccgga aaacattttt aatgtatcta tgataccgtg     7320

gtcaaccttc gatggcttta atctgaattt gcagaaagga tatgattatt tgattcctat     7380

ttttactatg gggaaatatt ataaagaaga taacaaaatt atacttcctt tggcaattca     7440

agttcatcac gcagtatgtg acggatttca catttgccgt tttgtaaacg aattgcagga     7500

attgataaat agttaacttc aggtttgtct gtaactaaaa acaagtattt aagcaaaaac     7560

atcgtagaaa tacggtgttt tttgttaccc taaaatctac aattttatac ataaccacag     7620

gttagtacaa agaccttgtg tttctttttg aaaggcttaa aacaaggatt tttccttgat     7680

ttaagccccg aaaagcaaca caaccaaggt tttagtatca atctgtggtt tttatatttt     7740

cagagaccgg tcaggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     7800

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     7860

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     7920

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     7980

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     8040

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     8100

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg     8160

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     8220

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     8280

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     8340

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc     8400

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     8460

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     8520

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     8580

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     8640

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     8700

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     8760

gcggaagcag taagacgggt aagcctgttg atgataccgc tgccttactg ggtgcattag     8820

ccagtctgaa tgacctgtca cgggataatt cctaactcac attaattgcg ttgcgctcac     8880

tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg     8940

cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg     9000

ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg     9060

ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttgacggcgg gatataacat     9120

gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg     9180

gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca     9240

gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc     9300

cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag     9360

ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc     9420

tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa     9480

ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg     9540

caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca     9600

ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt     9660

tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg     9720

acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac     9780

tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc     9840

gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa     9900

acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcata     9960

tgttgcacct ctactttaat aatttttaac ttttatatat gattaattta attgtttgtt    10020

aaatttatat caatcaatgc tatgaatatt tctttatacc ttattgtaac aaaaaaatat    10080

tggaaatgtt gaattttcag aatattattt ttattatatt attaatttta tatattcatt    10140

tttataagat ttcacaacac gaacgtaata taatatatct tcctcatctt ctgaaaagat    10200

tatactaatt ctattcatgt tacttataat cttattttgg taaatcgaat ttttcaatta    10260

tatgttcggc aacctttatc ccatcaacag ccgctgatat tataccacct gcaaatcctg    10320

ccccttctcc agttggataa agtccgcata catttatact ttcaagtgaa gcatttctat    10380

tcaatctaac tggtgctgat gttcttgtct caattcccgt taaaattgca tcttctcttg    10440

catacccttt tatcttttta tcaaaattta taattccttc tttaagagcc tctacaacat    10500

aatcaggtaa acattctttt aattccctga attatctgca gaattcgccc ttcctgcttc    10560

ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt    10620

tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata    10680

ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg    10740

gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat    10800

gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag    10860

gtgtactgcc ttccagacga acgaagagcg attgaggaaa aggcggcggc ggccggcatg    10920

agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac    10980

tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg    11040

ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc    11100

gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg    11160

gtccgcccga gggcagagcc atgacttttt tagccgctaa aacggccggg gggtgcgcgt    11220

gattgccaag cacgtcccca tgcgctccat caagaagagc gacttcgcgg agctggtgaa    11280

gtacatcacc gacgagcaag gcaagaccga tccccatccc gaagtggtca gactggaaaa    11340

tcagagggca ggaactgcga acagcaaaaa gtcagatagc accacatagc agacccgcca    11400

taaaacgccc tgagagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg    11460

aatccataaa aggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta    11520

atgcagagct tactccccat ccccccagtg aattccccgg atcgagatag tatatgatgc    11580

atattcttta aatatagata aagttataga agcaatagaa gatttaggat ttactgtaat    11640

ataaattaca cttttaaaaa gtttaaaaac atgatacaat aagttatggt tggaattgtt    11700

atccgctcac aattccaact tatgattaaa attttaagga ggtgtatttc atatgaccat    11760

gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacgtcac gcgtccatgg    11820

agatctcgag gcctgcaggc atgca                                          11845


<210>  9
<211>  3015
<212>  DNA
<213>  Clostridium acetobutylicum ATCC 824

<400>  9
attttacttt attctaataa tacgtaatac acccacttat aactagtatt tggcaataaa       60

aatagttata atcattaatt attgttaaat gtttgacaat ctttaattac tgttatataa      120

taatattata gaaaataaaa tgactgcata attttactat agaaatacaa gcgttaaata      180

tgtacatatc aacggtttat cacattagaa gtaaataatg taaggaaacc acactctata      240

atttataagg catcaaagtg tgttatataa tacaataagt tttatttgca atagtttgtt      300

aaatatcaaa ctaataataa attttataaa ggagtgtata taaatgaaag ttacaaatca      360

aaaagaacta aaacaaaagc taaatgaatt gagagaagcg caaaagaagt ttgcaaccta      420

tactcaagag caagttgata aaatttttaa acaatgtgcc atagccgcag ctaaagaaag      480

aataaactta gctaaattag cagtagaaga aacaggaata ggtcttgtag aagataaaat      540

tataaaaaat cattttgcag cagaatatat atacaataaa tataaaaatg aaaaaacttg      600

tggcataata gaccatgacg attctttagg cataacaaag gttgctgaac caattggaat      660

tgttgcagcc atagttccta ctactaatcc aacttccaca gcaattttca aatcattaat      720

ttctttaaaa acaagaaacg caatattctt ttcaccacat ccacgtgcaa aaaaatctac      780

aattgctgca gcaaaattaa ttttagatgc agctgttaaa gcaggagcac ctaaaaatat      840

aataggctgg atagatgagc catcaataga actttctcaa gatttgatga gtgaagctga      900

tataatatta gcaacaggag gtccttcaat ggttaaagcg gcctattcat ctggaaaacc      960

tgcaattggt gttggagcag gaaatacacc agcaataata gatgagagtg cagatataga     1020

tatggcagta agctccataa ttttatcaaa gacttatgac aatggagtaa tatgcgcttc     1080

tgaacaatca atattagtta tgaattcaat atacgaaaaa gttaaagagg aatttgtaaa     1140

acgaggatca tatatactca atcaaaatga aatagctaaa ataaaagaaa ctatgtttaa     1200

aaatggagct attaatgctg acatagttgg aaaatctgct tatataattg ctaaaatggc     1260

aggaattgaa gttcctcaaa ctacaaagat acttataggc gaagtacaat ctgttgaaaa     1320

aagcgagctg ttctcacatg aaaaactatc accagtactt gcaatgtata aagttaagga     1380

ttttgatgaa gctctaaaaa aggcacaaag gctaatagaa ttaggtggaa gtggacacac     1440

gtcatcttta tatatagatt cacaaaacaa taaggataaa gttaaagaat ttggattagc     1500

aatgaaaact tcaaggacat ttattaacat gccttcttca cagggagcaa gcggagattt     1560

atacaatttt gcgatagcac catcatttac tcttggatgc ggcacttggg gaggaaactc     1620

tgtatcgcaa aatgtagagc ctaaacattt attaaatatt aaaagtgttg ctgaaagaag     1680

ggaaaatatg ctttggttta aagtgccaca aaaaatatat tttaaatatg gatgtcttag     1740

atttgcatta aaagaattaa aagatatgaa taagaaaaga gcctttatag taacagataa     1800

agatcttttt aaacttggat atgttaataa aataacaaag gtactagatg agatagatat     1860

taaatacagt atatttacag atattaaatc tgatccaact attgattcag taaaaaaagg     1920

tgctaaagaa atgcttaact ttgaacctga tactataatc tctattggtg gtggatcgcc     1980

aatggatgca gcaaaggtta tgcacttgtt atatgaatat ccagaagcag aaattgaaaa     2040

tctagctata aactttatgg atataagaaa gagaatatgc aatttcccta aattaggtac     2100

aaaggcgatt tcagtagcta ttcctacaac tgctggtacc ggttcagagg caacaccttt     2160

tgcagttata actaatgatg aaacaggaat gaaataccct ttaacttctt atgaattgac     2220

cccaaacatg gcaataatag atactgaatt aatgttaaat atgcctagaa aattaacagc     2280

agcaactgga atagatgcat tagttcatgc tatagaagca tatgtttcgg ttatggctac     2340

ggattatact gatgaattag ccttaagagc aataaaaatg atatttaaat atttgcctag     2400

agcctataaa aatgggacta acgacattga agcaagagaa aaaatggcac atgcctctaa     2460

tattgcgggg atggcatttg caaatgcttt cttaggtgta tgccattcaa tggctcataa     2520

acttggggca atgcatcacg ttccacatgg aattgcttgt gctgtattaa tagaagaagt     2580

tattaaatat aacgctacag actgtccaac aaagcaaaca gcattccctc aatataaatc     2640

tcctaatgct aagagaaaat atgctgaaat tgcagagtat ttgaatttaa agggtactag     2700

cgataccgaa aaggtaacag ccttaataga agctatttca aagttaaaga tagatttgag     2760

tattccacaa aatataagtg ccgctggaat aaataaaaaa gatttttata atacgctaga     2820

taaaatgtca gagcttgctt ttgatgacca atgtacaaca gctaatccta ggtatccact     2880

tataagtgaa cttaaggata tctatataaa atcattttaa aaaataaaga atgtaaaata     2940

gtctttgctt cattatatta gcttcatgaa gcacatagac tattttacat tttactcttg     3000

ttttttatct ttcaa                                                      3015


<210>  10
<211>  1389
<212>  DNA
<213>  Clostridium tyrobutyricum

<400>  10
atattcagcg aaaatagtat attatataat tataaattga tgaatagcta gagtggtcag       60

acctcctagc tattgtttta gaaaactttg tgtttttttt aacaaaaata ttgataaatt      120

tttaattatc tagtataatg aagttgttgg taaaaaggtt tgtaatcaat ttaaatttta      180

aaatataaat atttaggagg aatagtcatg agtgaagttg taattgcaag tgccgttaga      240

acagcaatag gtaaatttgg aggtagcttg aagtcagttc cagcaactga tttaggagct      300

ttggttataa aagaagcatt aaaaagagct aatgttaaac cagaacttgt agatgaagtt      360

gtaatgggta atgtattaca ggcaggttta ggtcagaatc cagcaagaca agcattgata      420

aaatcaggta tacctaatac agtccctgga tttactataa ataaagtttg tggttcagga      480

cttagagcag taagtttagc agctcaaatg ataaaagctg gcgatgatga tatcgttgta      540

gctggtggaa tggaaaatat gtctgctgct ccatatgtaa tgcctagtgc aagatgggga      600

caaagaatgt ttgatggtaa gattatagat gaaatggtaa aagacggact ttgggatgct      660

tttaataact atcacatggg tattacggct gaaaatatag cagaaaagtg gaacataaca      720

agacaaatgc aggatgaatt tgcagctgca tcacaacaaa aagcagtagc agctataaaa      780

tctggcaagt ttaaagatga aatagttcca gtagtaatca aggatagaaa aaagggagaa      840

atagtatttg atactgatga attccctaga gatggtgtaa ctgtcgaagg aatatcaaaa      900

ttaaaaccag catttaagaa ggatggtgga actgttacag ctgccaatgc ttcaggtata      960

aatgatgcag ctgcagcatt agttataatg agtgcagaca aagcaaaaga attgggaatt     1020

aaaccacttg ctaaaattac ttcttatgga tcaaaaggct tggatcctag cataatggga     1080

tatggaccat tccatgctac aaaactagca cttaaaaaag ctaatttaac tgtagatgat     1140

ttagatttaa tagaagcaaa cgaagcattt gctgctcaga gtttagctgt tgcaaaagac     1200

ttgaaatttg atatgagcaa agtaaatgta aatggaggag caatagcact tggacatcct     1260

attggagctt caggcgcaag aatacttact actttgcttt atgaaatgca gaaaagagat     1320

tcaaaacgtg gtttagctac attatgtata ggcggtggaa tgggaactgc tataatcgtt     1380

gaaagataa                                                             1389


<210>  11
<211>  564
<212>  DNA
<213>  Clostridium tyrobutyricum

<400>  11
gatacagcat tccaccagac catgcctgca catgcatatc gttatgcttt accaaagttc       60

ctgtatacac aacacaacgt tcgtcgttat ggtttccatg gtaccagtca tgcctatgtt      120

tctgaacgtg gttctgaact cgcggggagc tataaacatg gcggttggtt aacagcacac      180

ttaggcaatg gtagctccac ttgcgccatt tggaatggtc aaagtgtcga tacctccatg      240

ggactgactc cgcttgaagg cgtggtcatg ggtactcgta gtggtgatgt tgatccaagt      300

attcatagct tccttgcttc aaatctgggc tgggacatct ataaaattga taaaatgctg      360

aacagtgaat caggcttact gggcttatca gatctttcaa atgatatgcg taccttgatt      420

gaagcttcag aacaaggcaa tgaagatgcg actttggcga ttgaagtatt ctgttatcgt      480

ttagccaaat cacttgcggc attaagttgc ggtttaccgc gtatcgatgg tttattcttt      540

acaggaggta ttggagaaaa ctct                                             564


<210>  12
<211>  732
<212>  DNA
<213>  Clostridium tyrobutyricum

<400>  12
gagttgagga aacacaaggg tatgactcct gataaggcga ataagatagt tagagaccca       60

ttgtactttg caacaatgat ggtcaaacta ggagatgctg atggattggt atctggttct      120

atacatacta caggcgatct tttgagacca ggtcttcaaa ttgtaaaaac tgctccagga      180

acttcagtag tttcaagcat atttatgatg gaagttccaa attgtgattt aggtgataat      240

ggtttcttgt tgttttcaga ttgtgctgta aatcctgtac ctaatactga acaattagct      300

gcaatagcta ttagtactgc agaaactgca aaaagtttat gtggtatgga tccaaaagta      360

gctatgcttt cattttcaac taagggaagt gcacaacatg aaaatgtaga taaggtaaga      420

gaagcaacca aacttgctaa acaaatgcaa ccagatctta aaatagacgg agaacttcaa      480

ttggatgctt ctttaataca ggaagttgca aatttaaaag cacctggtag tcctgtagca      540

ggaaaagcaa atgtacttat attccctgaa cttcaagcag gaaatattgg atataaatta      600

gttcaaagat ttgcaaaagc ggaagcaata ggacctatat gtcaaggctt tgcaaaacca      660

ataaatgatt tgtcaagagg atgcagttct gacgatatag taaatgtagt agcaattaca      720

gcagcacagg ct                                                          732


