                         SEQUENCE LISTING

<110>  Codexis, Inc.
       Haerizadeh, Farzad
       Valle, Fernando
       Cottarel, Guillaume
 
<120>  USE OF AN ENDOGENOUS 2--MICRON YEAST PLASMID FOR GENE 
       OVEREXPRESSION

<130>  153-000210PC
<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  6318
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  1
tttggttttc ttttaccagt attgttcgtt tgataatgta ttcttgctta ttacattata       60

aaatctgtgc agatcacatg tcaaaacaac tttttatcac aagatagtac cgcaaaacga      120

acctgcgggc cgtctaaaaa ttaaggaaaa gcagcaaagg tgcattttta aaatatgaaa      180

tgaagatacc gcagtaccaa ttattttcgc agtacaaata atgcgcggcc ggtgcatttt      240

tcgaaagaac gcgagacaaa caggacaatt aaagttagtt tttcgagtta gcgtgtttga      300

atactgcaag atacaagata aatagagtag ttgaaactag atatcaattg cacacaagat      360

cggcgctaag catgccacaa tttgatatat tatgtaaaac accacctaag gtgcttgttc      420

gtcagtttgt ggaaaggttt gaaagacctt caggtgagaa aatagcatta tgtgctgctg      480

aactaaccta tttatgttgg atgattacac ataacggaac agcaatcaag agagccacat      540

tcatgagcta taatactatc ataagcaatt cgctgagttt cgatattgtc aataaatcac      600

tccagtttaa atacaagacg caaaaagcaa caattctgga agcctcatta aagaaattga      660

ttcctgcttg ggaatttaca attattcctt actatggaca aaaacaccaa tctgatatca      720

ctgatattgt aagtagtttg caattacagt tcgaatcatc ggaagaagca gataagggaa      780

atagccacag taaaaaaatg cttaaagcac ttctaagtga gggtgaaagc atctgggaga      840

tcactgagaa aatactaaat tcgtttgagt atacttcgag atttacaaaa acaaaaactt      900

tataccaatt cctcttccta gctactttca tcaattgtgg aagattcagc gatattaaga      960

acgttgatcc gaaatcattt aaattagtcc aaaataagta tctgggagta ataatccagt     1020

gtttagtgac agagacaaag acaagcgtta gtaggcacat atacttcttt agcgcaaggg     1080

gtaggatcga tccacttgta tatttggatg aatttttgag gaattctgaa ccagtcctaa     1140

aacgagtaaa taggaccggc aattcttcaa gcaataaaca ggaataccaa ttattaaaag     1200

ataacttagt cagatcgtac aataaagctt tgaagaaaaa tgcgccttat tcaatctttg     1260

ctataaaaaa tggcccaaaa tctcacattg gaagacattt gatgacctca tttctttcaa     1320

tgaagggcct aacggagttg actaatgttg tgggaaattg gagcgataag cgtgcttctg     1380

ccgtggccag gacaacgtat actcatcaga taacagcaat acctgatcac tacttcgcac     1440

tagtttctcg gtactatgca tatgatccaa tatcaaagga aatgatagca ttgaaggatg     1500

agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa     1560

gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat     1620

cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact atgccgttct     1680

tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct     1740

gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt     1800

tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaaaac     1860

caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct     1920

actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat     1980

atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat     2040

ctaaactcga cctctacatc aacaggcttc caatgctctt caaattttac tgtcaagtag     2100

acccatacgg ctgtaatatg ctgctcttca taatgtaagc ttatctttat cgaatcgtgt     2160

gaaaaactac taccgcgata aacctttacg gttccctgag attgaattag ttcctttagt     2220

atatgataca agacactttt gaactttgta cgacgaattt tgaggttcgc catcctctgg     2280

ctatttccaa ttatcctgtc ggctattatc tccgcctcag tttgatcttc cgcttcagac     2340

tgccattttt cacataatga atctatttca ccccacaatc cttcatccgc ctccgcatct     2400

tgttccgtta aactattgac ttcatgttgt acattgttta gttcacgaga agggtcctct     2460

tcaggcggta gctcctgatc tcctatatga cctttatcct gttctctttc cacaaactta     2520

gaaatgtatt catgaattat ggagcaccta ataacattct tcaaggcgga gaagtttggg     2580

ccagatgccc aatatgcttg acatgaaaac gtgagaatga atttagtatt attgtgatat     2640

tctgaggcaa ttttattata atctcgaaga taagagaaga atgcagtgac ctttgtattg     2700

acaaatggag attccatgta tctaaaaaat acgcctttag gccttctgat accctttccc     2760

ctgcggttta gcgtgccttt tacattaata tctaaaccct ctccgatggt ggcctttaac     2820

tgactaataa atgcaaccga tataaactgt gataattctg ggtgatttat gattcgatcg     2880

acaattgtat tgtacactag tgcaggatca ggccaatcca gttctttttc aattaccggt     2940

gtgtcgtctg tattcagtac atgtccaaca aatgcaaatg ctaacgtttt gtatttctta     3000

taattgtcag gaactggaaa agtccccctt gtcgtctcga ttacacacct actttcatcg     3060

tacaccatag gttggaagtg ctgcataata cattgcttaa tacaagcaag cagtctctcg     3120

ccattcatat ttcagttatt ttccattaca gctgatgtca ttgtatatca gcgctgtaaa     3180

aatctatctg ttacagaagg ttttcgcggt ttttataaac aaaactttcg ttacgaaatc     3240

gagcaatcac cccagctgcg tatttggaaa ttcgggaaaa agtagagcaa cgcgagttgc     3300

attttttaca ccataatgca tgattaactt cgagaaggga ttaaggctaa tttcactagt     3360

atgtttcaaa aacctcaatc tgtccattga atgccttata aaacagctat agattgcata     3420

gaagagttag ctactcaatg ctttttgtca aagcttactg atgatgatgt gtctactttc     3480

aggcgggtct gtagtaagga gaatgacatt ataaagctgg cacttagaat tccacggact     3540

atagactata ctagtatact ccgtctactg tacgatacac ttccgctcag gtccttgtcc     3600

tttaacgagg ccttaccact cttttgttac tctattgatc cagctcagca aaggcagtgt     3660

gatctaagat tctatcttcg cgatgtagta aaactagcta gaccgagaaa gagactagaa     3720

atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac gctgcagctt     3780

ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca aactgtttta     3840

cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt     3900

ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg     3960

gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc     4020

ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc     4080

tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta     4140

atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg     4200

ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag     4260

cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga     4320

gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc     4380

gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct     4440

ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta     4500

ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga     4560

ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat     4620

accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt     4680

ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg     4740

tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg     4800

tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt     4860

tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca     4920

aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt     4980

acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa     5040

aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa     5100

gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact     5160

gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc     5220

atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg     5280

tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca     5340

gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc     5400

aatgctatca tttcctttga tattggatca taccctagaa gtattacgtg attttctgcc     5460

ccttaccctc gttgctactc tccttttttt cgtgggaacc gctttagggc cctcagtgat     5520

ggtgttttgt aatttatatg ctcctcttgc atttgtgtct ctacttcttg ttcgcctgga     5580

gggaacttct tcatttgtat tagcatggtt cacttcagtc cttccttcca actcactctt     5640

tttttgctgt aaacgattct ctgccgccag ttcattgaaa ctattgaata tatcctttag     5700

agattccggg atgaataaat cacctattaa agcagcttga cgatctggtg gaactaaagt     5760

aagcaattgg gtaacgacgc ttacgagctt cataacatct tcttccgttg gagctggtgg     5820

gactaataac tgtgtacaat ccatttttct catgagcatt tcggtagctc tcttcttgtc     5880

tttctcgggc aatcttccta ttattatagc aatagatttg tatagttgct ttctattgtc     5940

taacagcttg ttattctgta gcatcaaatc tatggcagcc tgacttgctt cttgtgaaga     6000

gagcatacca tttccaatcg aatcaaacct ttccttaacc atcttcgcag caggcaaaat     6060

tacctcagca ctggagtcag aagatacgct ggaatcttct gcgctagaat caagaccata     6120

cggcctaccg gttgtgagag attccatggg ccttatgaca tatcctggaa agagtagctc     6180

atcagactta cgtttactct ctatatcaat atctacatca ggagcaatca tttcaataaa     6240

cagccgacat acatcccaga cgctataagc tgtacgtgct tttaccgtca gattcttggc     6300

tgtttcaatg tcgtccat                                                   6318


<210>  2
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  2
ggtagctcct gatctcctat atgacc                                            26


<210>  3
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  3
atgcagcact tccaacctat ggtgtacg                                          28


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  4
ggttcacttc agtccttcct tccaactcac                                        30


<210>  5
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucletoide for PCR

<400>  5
aaagcacgta cagcttatag cgtctggg                                          28


<210>  6
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  6
atgcagcact tccaacctat ggtgtacg                                          28


<210>  7
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  7
ggtagctcct gatctcctat atgacc                                            26


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  8
aaagcacgta cagcttatag cgtctggg                                          28


<210>  9
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  9
ggttcacttc agtccttcct tccaactcac                                        30


<210>  10
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  10
gtacactagt gcaggatcag gccaatcc                                          28


<210>  11
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  11
gctcagcaaa ggcagtgtga tctaag                                            26


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  12
ttttgttcta caaaaatgca tcccg                                             25


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  13
agatgcaagt tcaaggagcg aaaggtgg                                          28


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  14
ggaaggactg aagtgaacca tgc                                               23


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  15
gtctctactt cttgttcgcc tggaggg                                           27


<210>  16
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  16
gttgttttga catgtgatct gcacag                                            26


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  17
cggccggtgc atttttcgaa agaacgcg                                          28


<210>  18
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  18
gggcctaacg gagttgacta atgttgtg                                          28


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  19
gtttcaggga aaactcccag gt                                                22


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  20
ggtcatatag gagatcagga gctacc                                            26


<210>  21
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  21
cccagacgct ataagctgta cgtgcttt                                          28


<210>  22
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  22
tgttattctg tagcatcaaa tctatgg                                           27


<210>  23
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  23
agattgatgt ttttgtccat agtaagg                                           27


<210>  24
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  24
tataagctgt acgtgctttt accg                                              24


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  25
ccacaaactg acgaacaagc                                                   20


<210>  26
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  26
acctgcgggc cgtctaaaaa ttaaggaaaa gcagcaaagg tgcattttta aaatatgaaa       60

tgaagctcac agacgcgttg aattgtccc                                         89


<210>  27
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  27
cgcgttcttt cgaaaaatgc accggccgcg cattatttgt actgcgaaaa taattggtac       60

tgcggtatgg ttaaaaaatg agctgattta ac                                     92


<210>  28
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  28
ttatcacaag atagtaccgc aaaacgaacc tgcgggccgt ctaaaaatta aggaaaagca       60

gcaaacatct gtgcggtatt tcacaccgc                                         89


<210>  29
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  29
cattatttgt actgcgaaaa taattggtac tgcggtatct tcatttcata ttttaaaaat       60

gcaccgaagc aaaaattacg gctcct                                             85


<210>  30
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  30
tgtgcagatc acatgtcaaa acaacttttt atcacaagat agtaccgcaa aacgaacctg       60

cgggccatct gtgcggtatt tcacaccgc                                         89


<210>  31
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  31
actgcggtat cttcatttca tattttaaaa atgcaccttt gctgcttttc cttaattttt       60

agacggaagc aaaaattacg gctcct                                            86


<210>  32
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  32
ttatcacaag atagtaccgc aaaacgaacc tgcgggccgt ctaaaaatta aggaaaagca       60

gcaaagggaa caaaagctgg agctccatag c                                      91


<210>  33
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  33
cattatttgt actgcgaaaa taattggtac tgcggtatct tcatttcata ttttaaaaat       60

gcaccgaagc aaaaattacg gctcct                                            86


<210>  34
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  34
tgtgcagatc acatgtcaaa acaacttttt atcacaagat agtaccgcaa aacgaacctg       60

cgggcgggaa caaaagctgg agctccatag c                                      91


<210>  35
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized oligonucleotide for PCR

<400>  35
actgcggtat cttcatttca tattttaaaa atgcaccttt gctgcttttc cttaattttt       60

agacggaagc aaaaattacg gctcct                                            86


