

SEQUENCE LISTING

<110>  Universit de Liege et al.
 
<120>  COMBINATION OF AN AGONISTIC ANTI-CD40 MONOCLONAL ANTIBODY OR A CD40 LIGAND AND INACTIVATED OR ATTENUATED BACTERIA FOR USE IN THE TREATMENT AND/OR PREVENTION OF MASTITIS

<130>  S1007 PCT S3

<160>  27    

<170>  PatentIn version 3.4

<210>  1
<211>  5
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of CDR1"

<400>  1

Ser Tyr Ala Met Ser 
1               5   


<210>  2
<211>  16
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of CDR2"


<400>  2

Ser Ile Gly Ser Gly Gly Gly Thr Tyr Tyr Pro Asp Ser Val Lys Asp 
1               5                   10                  15      


<210>  3
<211>  13
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of CDR3"


<400>  3

Ala Tyr Tyr Arg Asn His Arg Gly Ser Val Met Asp Tyr 
1               5                   10              


<210>  4
<211>  15
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of CDR1'"


<400>  4

Lys Ala Ser Gln Thr Val Asp Tyr Asp Gly Asp Ser Tyr Met Asn 
1               5                   10                  15  


<210>  5
<211>  7
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of CDR2'"

<400>  5

Ser Ala Ser Asn Leu Glu Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of CDR3'"

<400>  6

Gln Gln Ser Thr Glu Asp Pro Pro Thr 
1               5                   


<210>  7
<211>  363
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding VH"

<400>  7
gaagtgaagc tggtggagtc tgggggagtc ttagtgaagc ctggagggtc cctgaaactc     60

tcctgtgcag cctctggatt cactttcagt agctatgcca tgtcttgggt tcgccagact    120

ccagaaaaga ggctggagtg ggtcgcatct attggtagtg gtggtggaac ttactatcca    180

gacagtgtga agggccgatt caccatctcc agaaataatg ccaggaacat cctgtacctg    240

caaatgagca gtctgaggtc tgaggacacg gccatgtatt actgtgcaag agcctactat    300

aggaaccacc gagggtctgt tatggactac tggggtcaag gaacctcagt caccgtctcc    360

tca                                                                  363


<210>  8
<211>  121
<212>  PRT
<213>  Mus musculus

<220>
<223>  /note="Description: amino acid sequence of VH"

<400>  8

Glu Val Lys Leu Val Glu Ser Gly Gly Val Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Gly Ser Gly Gly Gly Thr Tyr Tyr Pro Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asn Asn Ala Arg Asn Ile Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Ala Tyr Tyr Arg Asn His Arg Gly Ser Val Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120     


<210>  9
<211>  333
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding VL"

<400>  9
gacattgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc     60

atctcctgca aggccagcca aactgttgat tatgatggtg atagttatat gaactggtac    120

caacagaaac caggacagcc acccaaagtc ctcatctatt ctgcatccaa tctggaatct    180

gggatcccag ccaggtttag tggcagtggg tctgggacag acttcaccct caacatccat    240

cctgtggagg aggaggatgc tgcaacctat tactgtcagc aaagtactga ggatcctccg    300

acgttcggtg gaggcaccaa gctggaaatc aaa                                 333


<210>  10
<211>  111
<212>  PRT
<213>  Mus musculus


<220>
<223>  /note="Description: amino acid sequence of VL"

<400>  10

Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Thr Val Asp Tyr Asp 
            20                  25                  30          


Gly Asp Ser Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Val Leu Ile Tyr Ser Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Thr 
                85                  90                  95      


Glu Asp Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  11
<211>  45
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding CDR1'"

<400>  11
aaggccagcc aaactgttga ttatgatggt gatagttata tgaac                     45


<210>  12
<211>  21
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding CDR2'"

<400>  12
tctgcatcca atctggaatc t                                               21


<210>  13
<211>  27
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding CDR3'"

<400>  13
cagcaaagta ctgaggatcc tccgacg                                         27


<210>  14
<211>  15
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding CDR1"

<400>  14
agctatgcca tgtct                                                      15


<210>  15
<211>  48
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding CDR2"

<400>  15
tctattggta gtggtggtgg aacttactat ccagacagtg tgaagggc                  48


<210>  16
<211>  39
<212>  DNA
<213>  Mus musculus

<220>
<223>  /note="Description: nucleic acid sequence encoding CDR3"

<400>  16
gcctactata ggaaccaccg agggtctgtt atggactac                            39


<210>  17
<211>  843
<212>  DNA
<213>  Bos taurus

<220>
<223>  /note="Description: cDNA sequence encoding bovine CD40"

<400>  17
atggttcgtt tgccactgca gtgtctcttc tggggcttct ttctgaccgc cgtccactca     60

gaaccagcca ctgcttgtgg agagaagcaa tacccagtga acagtctttg ctgtgatttg    120

tgcccgccgg gacagaaact ggtgaacgac tgcacagagg tcagcaaaac agaatgccag    180

tcctgcggta aaggcgaatt cttgtccacc tggaacagag agaaatactg tcacgagcac    240

agatactgca accccaacct agggctccgg atccagagcg agggtacctt gaatacagac    300

accatttgtg tatgtgtcga aggccaacac tgtaccagtc acacctgcga aagttgcacg    360

ccccacagct tgtgtctccc tggcttcggg gtcaagcaga tcgctacagg gcttttggat    420

accgtctgtg aaccctgccc gctcggcttc ttctccaacg tgtcatctgc ttttgaaaag    480

tgtcaccgtt ggacaagctg cgagagaaaa ggcctggtgg aacaacacgt ggggacgaac    540

aagacagatg ttgtctgcgg tttccagagt cggatgagga ccctggtggt gatccccgtc    600

acgatgggag tcttgtttgc tgtcctgttg gtatctgcct gtatcaggaa cataaccaag    660

aagcggcagg ctaaggccct gcaccctatg gctgaaaggc aggatcccgt ggagacgatt    720

gatccggagg attttcccgg cccccacccg cctcctccgg tgcaagagac cttatgctgg    780

tgtcagccgg tcgcccagga ggacggcaaa gagagccgca tctccgtgca ggagcgagaa    840

tga                                                                  843


<210>  18
<211>  280
<212>  PRT
<213>  Bos taurus

<220>
<223>  /note="Description: bovine CD40 amino acid sequence"

<400>  18

Met Val Arg Leu Pro Leu Gln Cys Leu Phe Trp Gly Phe Phe Leu Thr 
1               5                   10                  15      


Ala Val His Ser Glu Pro Ala Thr Ala Cys Gly Glu Lys Gln Tyr Pro 
            20                  25                  30          


Val Asn Ser Leu Cys Cys Asp Leu Cys Pro Pro Gly Gln Lys Leu Val 
        35                  40                  45              


Asn Asp Cys Thr Glu Val Ser Lys Thr Glu Cys Gln Ser Cys Gly Lys 
    50                  55                  60                  


Gly Glu Phe Leu Ser Thr Trp Asn Arg Glu Lys Tyr Cys His Glu His 
65                  70                  75                  80  


Arg Tyr Cys Asn Pro Asn Leu Gly Leu Arg Ile Gln Ser Glu Gly Thr 
                85                  90                  95      


Leu Asn Thr Asp Thr Ile Cys Val Cys Val Glu Gly Gln His Cys Thr 
            100                 105                 110         


Ser His Thr Cys Glu Ser Cys Thr Pro His Ser Leu Cys Leu Pro Gly 
        115                 120                 125             


Phe Gly Val Lys Gln Ile Ala Thr Gly Leu Leu Asp Thr Val Cys Glu 
    130                 135                 140                 


Pro Cys Pro Leu Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys 
145                 150                 155                 160 


Cys His Arg Trp Thr Ser Cys Glu Arg Lys Gly Leu Val Glu Gln His 
                165                 170                 175     


Val Gly Thr Asn Lys Thr Asp Val Val Cys Gly Phe Gln Ser Arg Met 
            180                 185                 190         


Arg Thr Leu Val Val Ile Pro Val Thr Met Gly Val Leu Phe Ala Val 
        195                 200                 205             


Leu Leu Val Ser Ala Cys Ile Arg Asn Ile Thr Lys Lys Arg Gln Ala 
    210                 215                 220                 


Lys Ala Leu His Pro Met Ala Glu Arg Gln Asp Pro Val Glu Thr Ile 
225                 230                 235                 240 


Asp Pro Glu Asp Phe Pro Gly Pro His Pro Pro Pro Pro Val Gln Glu 
                245                 250                 255     


Thr Leu Cys Trp Cys Gln Pro Val Ala Gln Glu Asp Gly Lys Glu Ser 
            260                 265                 270         


Arg Ile Ser Val Gln Glu Arg Glu 
        275                 280 


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Primer nucleic acid 
       sequence F-CD40"

<400>  19
atggttcgtt tgccactgca g                                               21


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Primer nucleic acid 
       R-CD40"

<400>  20
tcattctcgc tcctgcacgg                                                 20


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Nucleic acid 
       sequence coding for FLAG epitope"

<400>  21
gactacaagg acgacgatga caag                                            24


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Peptide amino acid 
       sequence of FLAG epitope"

<400>  22

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  23
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Primer nucleotide 
       sequence F1"

<400>  23
tttctgaccg ccgtccactc agactacaag gacgacgatg acaaggaacc agccactgct     60

tgtggagaga                                                            70


<210>  24
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Primer nucleotide 
       sequence F2"

<400>  24
atcgccacca tggttcgttt gccactgcag tgtctcttct ggggcttctt tctgaccgcc     60

gtccactcag ac                                                         72


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<221>  Source
<223>  /note="Description of Artificial Sequence: Primer nucleotide 
       sequence R"

<400>  25
ataatcattc tcgctcctgc acggagat                                        28


<210>  26
<211>  261
<212>  PRT
<213>  Homo Sapiens

<400>  26

Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu 
            100                 105                 110         


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     


<210>  27
<211>  261
<212>  PRT
<213>  Bos taurus

<400>  27

Met Ile Glu Thr Tyr Ser Gln Pro Ser Pro Arg Ser Val Ala Thr Gly 
1               5                   10                  15      


Pro Pro Val Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Lys Gly Glu Gly Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Arg Ser Arg Phe Glu Asp Leu Val Lys 
                85                  90                  95      


Asp Ile Met Gln Asn Lys Glu Val Lys Lys Lys Glu Lys Asn Phe Glu 
            100                 105                 110         


Met His Lys Gly Asp Gln Glu Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Pro Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Leu Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Ala Val Lys Arg Gln Gly Phe Tyr Tyr Ile Tyr Thr Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Thr Leu Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Ser Gly Ser Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ser Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Ser Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     





