                         SEQUENCE LISTING

<110>  MASSACHUSETTS INSTITUTE OF TECHNOLOGY
       Babb, Jonathan
       Li, Shuo
       Knight, Thomas
       Weiss, Ron
 
<120>  RAPID ASSEMBLY OF MULTIPLE ARBITRARY LENGTH DNA FRAGMENTS

<130>  M0656.70224WO00

<150>  US 61/386401
<151>  2010-09-24

<150>  US 61/454481
<151>  2011-03-18

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  n is a, c, g, or t

<400>  1
aattcgcggc cgcttctaga nnnnnnnnnn nn                                     32


<210>  2
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  2
caaagaauct taagcgccgg cgaagatct                                         29


<210>  3
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  n is a, c, g, or t

<400>  3
aattcgcggc cgcttctaga nnnnnnnnnn nn                                     32


<210>  4
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (30)..(41)
<223>  n is a, c, g, t or u

<400>  4
caaagaauct taagcgccgg cgaagatctn nnnnnnnnnn n                           41


<210>  5
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (21)..(28)
<223>  n is a, c, g, t or u

<400>  5
aattcgcggc cgcttctaga nnnnnnnn                                          28


<210>  6
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (30)..(37)
<223>  n is a, c, g, t or u

<400>  6
caaagaauct taagcgccgg cgaagatctn nnnnnnngat c                           41


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  7
agttacgcta gggataacag ggtaatatag                                        30


<210>  8
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  8
cgtaactata acggtcctaa ggtagcgaa                                         29


<210>  9
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  9
caccatctac ataacacctc aattacacca ttcatt                                 36


<210>  10
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  10
accaattctc tccaccttta tcctaccaaa attcct                                 36


<210>  11
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
ttccttctct tccaccaaat ttcaacacac taacat                                 36


<210>  12
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  12
tcctatctca ctaaccaaca tcatctccat acatca                                 36


<210>  13
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  13
ttcatacctc acaaaacctc ctttctcctt ctcatt                                 36


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  14
ctcttcacta cacaccatat tcctctccaa atacct                                 36


<210>  15
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  15
acacattttc acaccaattc cattaaccat catcaa                                 36


<210>  16
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  16
caacactttc aacactcaat acctacaact ctactc                                 36


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  17
tttctttcac caaaccactt tcatacaatc tcacat                                 36


<210>  18
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  18
ctagacacca tctacataac acctcaatta caccattcat ta                          42


<210>  19
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  19
ctagtaatga atggtgtaat tgaggtgtta tgtagatggt gtctag                      46


<210>  20
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  20
ctagacacca tctacataac acctcaatta caccattcat tactag                      46


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  21
ctagtaatga atggtgtaat tgaggtgtta tgtagatggt gt                          42


<210>  22
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  22
ctagagccaa ttctctccac ctttatccta ccaaaattcc ta                          42


<210>  23
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  23
ctagtaggaa ttttggtagg ataaaggtgg agagaattgg ctctag                      46


<210>  24
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  24
ctagagccaa ttctctccac ctttatccta ccaaaattcc tactag                      46


<210>  25
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  25
ctagtaggaa ttttggtagg ataaaggtgg agagaattgg ct                          42


<210>  26
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  26
ctagacacca tctacataac acctcaatta caccattcat ta                          42


<210>  27
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  27
ctagtaatga atggtgtaat tgaggtgtta tgtagatggt gt                          42


<210>  28
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  28
ctagagccaa ttctctccac ctttatccta ccaaaattcc ta                          42


<210>  29
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  29
ctagtaggaa ttttggtagg ataaaggtgg agagaattgg ct                          42


<210>  30
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  30
caccatctac ataacacctc aattacacca ttcatt                                 36


<210>  31
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  31
accaattctc tccaccttta tcctaccaaa attcct                                 36


<210>  32
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  32
aatgaatggt gtaattgagg tgttatgtag atggtg                                 36


<210>  33
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  33
aggaattttg gtaggataaa ggtggagaga attggt                                 36


<210>  34
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  34
caccatctac ataacacctc aattacacca ttcattataa                             40


<210>  35
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  35
aatgaatggt gtaattgagg tgttatgtag atggtg                                 36


<210>  36
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  36
caccatctac ataacacctc aattacacca ttcatt                                 36


<210>  37
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  37
caccatctac ataacacctc aattacacca ttcattttag                             40


<210>  38
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  38
accaattctc tccaccttta tcctaccaaa attcctataa                             40


<210>  39
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  39
aggaattttg gtaggataaa ggtggagaga attggt                                 36


<210>  40
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  40
accaattctc tccaccttta tcctaccaaa attcct                                 36


<210>  41
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  41
aggaattttg gtaggataaa ggtggagaga attggtttag                             40


<210>  42
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is deoxyuridine

<400>  42
ctagaagcgg ccgcgaattc naagaaac                                          28


<210>  43
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is deoxyuridine

<400>  43
tctagaagcg gccgcgaatt cnaagaaac                                         29


<210>  44
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is deoxyuridine

<400>  44
tactagtagc ggccgctgca gnaagaaac                                         29


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  45
gtttcttcct gcagcggccg ctac                                              24


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  n is deoxyuridine

<400>  46
ctgcagcggc cgctantagt a                                                 21


<210>  47
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is deoxyuridine

<400>  47
gtttcttcct gcagcggccg ctantagta                                         29


<210>  48
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  48
gtttcttcga attcgcggcc gcttctaga                                         29


<210>  49
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  49
aaaaaatcct tagctttcgc taaggatgat ttctggaatt                             40


<210>  50
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  50
tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt                             40
