                         LISTA DE SECUENCIAS

<110>  Fundacion Centro Nacional de Investigaciones Oncolgicas (CNIO)
 
<120>  Anticuerpo contra ephrin B2 y su uso

<130>  ES1599.10

<150>  ES201031402
<151>  2010-09-21

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  256
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro 
1               5                   10                  15      


Gly Ala Ser Val Arg Leu Ser Cys Glu Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Asp Tyr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 
        35                  40                  45              


Trp Met Gly Trp Met Asn Pro Ser Ser Gly Asn Thr Gly Tyr Ala Gln 
    50                  55                  60                  


Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asn Thr Ser Ile Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Asp Ile Thr Gly Thr Ala Thr Gly Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr Gln Ser 
    130                 135                 140                 


Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala Ser Ile Ser Cys 
145                 150                 155                 160 


Arg Ser Ser Gln Ser Leu Leu His Ser Asn Gly Tyr Asn Tyr Leu Asp 
                165                 170                 175     


Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Leu 
            180                 185                 190         


Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly 
        195                 200                 205             


Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp 
    210                 215                 220                 


Val Gly Val Tyr Tyr Cys Met Gln Gly Leu Leu Ser Pro Val Thr Phe 
225                 230                 235                 240 


Gly Gln Gly Thr Arg Leu Asp Ile Lys Arg Thr Val Ala Ala Pro Thr 
                245                 250                 255     


<210>  2
<211>  23
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador sentido para la comprobacin de la secuencia de los scFv

<400>  2
cataatgaaa tacctattgc cta                                               23


<210>  3
<211>  19
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador antisentido para la comprobacin de la secuencia de los 
       scFv.

<400>  3
cttattagcg tttgccatt                                                    19


<210>  4
<211>  28
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador sentido con la diana de RcaI.

<400>  4
cagtcatcat gaaataccta ttgcctac                                          28


<210>  5
<211>  28
<212>  DNA
<213>  Secuencia Artificial

<220>
<223>  Cebador antisentido con la diana de XhoI.

<400>  5
caccggactc gagtgcggcc ccattcag                                          28


<210>  6
<211>  20
<212>  PRT
<213>  Erwinia carotovora

<400>  6

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala 
            20  


<210>  7
<211>  301
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  scFv del clon B11 que incluye el pptido seal y los marcadores 
       c-myc y cadena de histidinas.

<400>  7

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Gln Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Arg Leu Ser Cys Glu Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asp Tyr Tyr Ile His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Met Gly Trp Met Asn Pro Ser Ser Gly Asn Thr 
65                  70                  75                  80  


Gly Tyr Ala Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg Asn Thr 
                85                  90                  95      


Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Asp Ile Thr Gly Thr Ala Thr Gly 
        115                 120                 125             


Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ile Val 
145                 150                 155                 160 


Leu Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala 
                165                 170                 175     


Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu His Ser Asn Gly Tyr 
            180                 185                 190         


Asn Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Gln Leu 
        195                 200                 205             


Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro Asp Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val 
225                 230                 235                 240 


Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Gly Leu Leu Ser 
                245                 250                 255     


Pro Val Thr Phe Gly Gln Gly Thr Arg Leu Asp Ile Lys Arg Thr Val 
            260                 265                 270         


Ala Ala Pro Thr Ala Ala Ala Glu Gln Lys Leu Ile Ser Glu Glu Asp 
        275                 280                 285             


Leu Asn Gly Ala Ala Leu Glu His His His His His His 
    290                 295                 300     


<210>  8
<211>  291
<212>  PRT
<213>  Secuencia Artificial

<220>
<223>  scFv del clon 2B1.

<400>  8

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Gln Val Gln Leu Val Gln Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Phe Thr Phe Asp Asp Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Ser Gly Ser Ile 
65                  70                  75                  80  


Gly Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                85                  90                  95      


Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Leu Tyr Tyr Cys Ala Arg Gly His Arg Thr Ser Asp Ala Phe 
        115                 120                 125             


Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Pro Val Leu 
145                 150                 155                 160 


Thr Gln Pro Pro Ser Val Ser Leu Ala Pro Gly Lys Thr Ala Arg Ile 
                165                 170                 175     


Thr Cys Gly Gly Asp Ser Ile Gly Leu Lys Ser Val His Trp Tyr Gln 
            180                 185                 190         


Gln Lys Pro Gly Gln Ala Pro Val Leu Val Met Ser Ser Asp Ser Asp 
        195                 200                 205             


Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Asn Ser Gly Asn 
    210                 215                 220                 


Thr Ala Thr Leu Thr Ile Thr Arg Val Glu Ala Gly Asp Glu Ala Asp 
225                 230                 235                 240 


Tyr Tyr Cys Gln Val Trp Asp Ser Ser Ser Asp His Met Val Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ala Ala Pro Ser 
            260                 265                 270         


Ala Ala Ala Leu Glu Asp Tyr Lys Asp Asp Asp Asp Lys His His His 
        275                 280                 285             


His His His 
    290     


