               SEQUENCE LISTING

<110> Autodisplay  Biotech GmbH

<120> p450 surface display

<130> 49058PWO

<150> EP10179060.8
<151> 2010-09-23

<160> 14

<170> BiSSAP 1.0

<210> 1
<211> 1242
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1242
<223> /mol_type="DNA"
      /note="CYP106A2 insert in plasmid pET-CYP13"
      /organism="Artificial Sequence"

<400> 1
ctcgagatgg aagaagttat tgcagtaaaa gaaattacta ggtttaaaac aaggacggag      60

gaatttagcc cgtacgcttg gtgtaaaagg atgttagaaa atgaccctgt gagttatcac     120

gaaggaacgg atacgtggaa tgtctttaaa tatgaagatg tgaagcgggt tctcagtgat     180

tataaacatt tttcaagtgt tcggaaacgg acgacgattt cagttggaac ggatagtgag     240

gaaggttctg tgcctgaaaa gatccaaatc actgaatcgg atccacctga tcatagaaaa     300

cgccgttcac tgctggcagc agcattcaca cctagaagtc ttcaaaactg ggaacctcgc     360

attcaggaaa ttgcagatga attgattgga caaatggatg gtggaacgga aatcgatatt     420

gtggcatcat tggcgagtcc gcttccgatc attgtcatgg ccgatttgat gggggttccc     480

tcgaaagatc gtttattgtt taagaaatgg gtggatacct tatttcttcc ttttgataga     540

gaaaagcaag aagaagtaga taaattgaag caagttgcag caaaagaata ctatcagtat     600

ttgtatccga ttgttgtgca aaaacgattg aacccggcgg atgatatcat ctcagatcta     660

ttgaagtcgg aagtggatgg ggaaatgttt acggatgatg aggttgtccg gacgaccatg     720

ctgattttag gtgcaggagt cgagacaacc agtcatttat tggccaatag cttttattcg     780

ctgctatatg atgacaaaga agtttatcaa gagttacatg aaaacctgga tttagttccg     840

caggcggtcg aagaaatgct ccgtttccga ttcaatctta ttaaattgga tcgcactgta     900

aaggaagata acgatctatt gggagtggaa ttgaaagaag gggatagcgt ggttgtttgg     960

atgagtgcag ctaatatgga cgaagagatg tttgaagacc ccttcacact taatatccac    1020

cgccctaata ataagaaaca tctcacattc ggtaatggcc ctcatttctg cctcggagca    1080

ccgctagcca ggctggaagc gaagattgcg cttactgcat tcctgaagaa attcaagcat    1140

attgaagcgg tgccatcgtt ccagttagaa gagaatctta ccgattcagc gaccggtcaa    1200

actttgacct cactaccgct taaggcaagc cgcatgggta cc                       1242


<210> 2
<211> 414
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..414
<223> /mol_type="protein"
      /note="CYP106A2 insert in plasmid pET-CYP13"
      /organism="Artificial Sequence"

<400> 2
Leu Glu Met Glu Glu Val Ile Ala Val Lys Glu Ile Thr Arg Phe Lys 
1               5                   10                   15    
Thr Arg Thr Glu Glu Phe Ser Pro Tyr Ala Trp Cys Lys Arg Met Leu 
            20                   25                  30        
Glu Asn Asp Pro Val Ser Tyr His Glu Gly Thr Asp Thr Trp Asn Val 
        35                   40                  45            
Phe Lys Tyr Glu Asp Val Lys Arg Val Leu Ser Asp Tyr Lys His Phe 
    50                   55                  60                
Ser Ser Val Arg Lys Arg Thr Thr Ile Ser Val Gly Thr Asp Ser Glu 
65                   70                  75                  80
Glu Gly Ser Val Pro Glu Lys Ile Gln Ile Thr Glu Ser Asp Pro Pro 
                85                   90                  95    
Asp His Arg Lys Arg Arg Ser Leu Leu Ala Ala Ala Phe Thr Pro Arg 
            100                  105                110        
Ser Leu Gln Asn Trp Glu Pro Arg Ile Gln Glu Ile Ala Asp Glu Leu 
        115                  120                125            
Ile Gly Gln Met Asp Gly Gly Thr Glu Ile Asp Ile Val Ala Ser Leu 
    130                  135                140                
Ala Ser Pro Leu Pro Ile Ile Val Met Ala Asp Leu Met Gly Val Pro 
145                  150                155                  160
Ser Lys Asp Arg Leu Leu Phe Lys Lys Trp Val Asp Thr Leu Phe Leu 
                165                  170                175    
Pro Phe Asp Arg Glu Lys Gln Glu Glu Val Asp Lys Leu Lys Gln Val 
            180                  185                190        
Ala Ala Lys Glu Tyr Tyr Gln Tyr Leu Tyr Pro Ile Val Val Gln Lys 
        195                  200                205            
Arg Leu Asn Pro Ala Asp Asp Ile Ile Ser Asp Leu Leu Lys Ser Glu 
    210                  215                220                
Val Asp Gly Glu Met Phe Thr Asp Asp Glu Val Val Arg Thr Thr Met 
225                  230                235                  240
Leu Ile Leu Gly Ala Gly Val Glu Thr Thr Ser His Leu Leu Ala Asn 
                245                  250                255    
Ser Phe Tyr Ser Leu Leu Tyr Asp Asp Lys Glu Val Tyr Gln Glu Leu 
            260                  265                270        
His Glu Asn Leu Asp Leu Val Pro Gln Ala Val Glu Glu Met Leu Arg 
        275                  280                285            
Phe Arg Phe Asn Leu Ile Lys Leu Asp Arg Thr Val Lys Glu Asp Asn 
    290                  295                300                
Asp Leu Leu Gly Val Glu Leu Lys Glu Gly Asp Ser Val Val Val Trp 
305                  310                315                  320
Met Ser Ala Ala Asn Met Asp Glu Glu Met Phe Glu Asp Pro Phe Thr 
                325                  330                335    
Leu Asn Ile His Arg Pro Asn Asn Lys Lys His Leu Thr Phe Gly Asn 
            340                  345                350        
Gly Pro His Phe Cys Leu Gly Ala Pro Leu Ala Arg Leu Glu Ala Lys 
        355                  360                365            
Ile Ala Leu Thr Ala Phe Leu Lys Lys Phe Lys His Ile Glu Ala Val 
    370                  375                380                
Pro Ser Phe Gln Leu Glu Glu Asn Leu Thr Asp Ser Ala Thr Gly Gln 
385                  390                395                  400
Thr Leu Thr Ser Leu Pro Leu Lys Ala Ser Arg Met Gly Thr 
                405                  410                

<210> 3
<211> 810
<212> PRT
<213> Escherichia coli

<220> 
<221> SOURCE
<222> 1..810
<223> /mol_type="protein"
      /note=" Outer membrane protein assembly factor, Omp85 homologue"
      /organism="Escherichia coli"

<400> 3
Met Ala Met Lys Lys Leu Leu Ile Ala Ser Leu Leu Phe Ser Ser Ala 
1               5                   10                   15    
Thr Val Tyr Gly Ala Glu Gly Phe Val Val Lys Asp Ile His Phe Glu 
            20                   25                  30        
Gly Leu Gln Arg Val Ala Val Gly Ala Ala Leu Leu Ser Met Pro Val 
        35                   40                  45            
Arg Thr Gly Asp Thr Val Asn Asp Glu Asp Ile Ser Asn Thr Ile Arg 
    50                   55                  60                
Ala Leu Phe Ala Thr Gly Asn Phe Glu Asp Val Arg Val Leu Arg Asp 
65                   70                  75                  80
Gly Asp Thr Leu Leu Val Gln Val Lys Glu Arg Pro Thr Ile Ala Ser 
                85                   90                  95    
Ile Thr Phe Ser Gly Asn Lys Ser Val Lys Asp Asp Met Leu Lys Gln 
            100                  105                110        
Asn Leu Glu Ala Ser Gly Val Arg Val Gly Glu Ser Leu Asp Arg Thr 
        115                  120                125            
Thr Ile Ala Asp Ile Glu Lys Gly Leu Glu Asp Phe Tyr Tyr Ser Val 
    130                  135                140                
Gly Lys Tyr Ser Ala Ser Val Lys Ala Val Val Thr Pro Leu Pro Arg 
145                  150                155                  160
Asn Arg Val Asp Leu Lys Leu Val Phe Gln Glu Gly Val Ser Ala Glu 
                165                  170                175    
Ile Gln Gln Ile Asn Ile Val Gly Asn His Ala Phe Thr Thr Asp Glu 
            180                  185                190        
Leu Ile Ser His Phe Gln Leu Arg Asp Glu Val Pro Trp Trp Asn Val 
        195                  200                205            
Val Gly Asp Arg Lys Tyr Gln Lys Gln Lys Leu Ala Gly Asp Leu Glu 
    210                  215                220                
Thr Leu Arg Ser Tyr Tyr Leu Asp Arg Gly Tyr Ala Arg Phe Asn Ile 
225                  230                235                  240
Asp Ser Thr Gln Val Ser Leu Thr Pro Asp Lys Lys Gly Ile Tyr Val 
                245                  250                255    
Thr Val Asn Ile Thr Glu Gly Asp Gln Tyr Lys Leu Ser Gly Val Glu 
            260                  265                270        
Val Ser Gly Asn Leu Ala Gly His Ser Ala Glu Ile Glu Gln Leu Thr 
        275                  280                285            
Lys Ile Glu Pro Gly Glu Leu Tyr Asn Gly Thr Lys Val Thr Lys Met 
    290                  295                300                
Glu Asp Asp Ile Lys Lys Leu Leu Gly Arg Tyr Gly Tyr Ala Tyr Pro 
305                  310                315                  320
Arg Val Gln Ser Met Pro Glu Ile Asn Asp Ala Asp Lys Thr Val Lys 
                325                  330                335    
Leu Arg Val Asn Val Asp Ala Gly Asn Arg Phe Tyr Val Arg Lys Ile 
            340                  345                350        
Arg Phe Glu Gly Asn Asp Thr Ser Lys Asp Ala Val Leu Arg Arg Glu 
        355                  360                365            
Met Arg Gln Met Glu Gly Ala Trp Leu Gly Ser Asp Leu Val Asp Gln 
    370                  375                380                
Gly Lys Glu Arg Leu Asn Arg Leu Gly Phe Phe Glu Thr Val Asp Thr 
385                  390                395                  400
Asp Thr Gln Arg Val Pro Gly Ser Pro Asp Gln Val Asp Val Val Tyr 
                405                  410                415    
Lys Val Lys Glu Arg Asn Thr Gly Ser Phe Asn Phe Gly Ile Gly Tyr 
            420                  425                430        
Gly Thr Glu Ser Gly Val Ser Phe Gln Ala Gly Val Gln Gln Asp Asn 
        435                  440                445            
Trp Leu Gly Thr Gly Tyr Ala Val Gly Ile Asn Gly Thr Lys Asn Asp 
    450                  455                460                
Tyr Gln Thr Tyr Ala Glu Leu Ser Val Thr Asn Pro Tyr Phe Thr Val 
465                  470                475                  480
Asp Gly Val Ser Leu Gly Gly Arg Leu Phe Tyr Asn Asp Phe Gln Ala 
                485                  490                495    
Asp Asp Ala Asp Leu Ser Asp Tyr Thr Asn Lys Ser Tyr Gly Thr Asp 
            500                  505                510        
Val Thr Leu Gly Phe Pro Ile Asn Glu Tyr Asn Ser Leu Arg Ala Gly 
        515                  520                525            
Leu Gly Tyr Val His Asn Ser Leu Ser Asn Met Gln Pro Gln Val Ala 
    530                  535                540                
Met Trp Arg Tyr Leu Tyr Ser Met Gly Glu His Pro Ser Thr Ser Asp 
545                  550                555                  560
Gln Asp Asn Ser Phe Lys Thr Asp Asp Phe Thr Phe Asn Tyr Gly Trp 
                565                  570                575    
Thr Tyr Asn Lys Leu Asp Arg Gly Tyr Phe Pro Thr Asp Gly Ser Arg 
            580                  585                590        
Val Asn Leu Thr Gly Lys Val Thr Ile Pro Gly Ser Asp Asn Glu Tyr 
        595                  600                605            
Tyr Lys Val Thr Leu Asp Thr Ala Thr Tyr Val Pro Ile Asp Asp Asp 
    610                  615                620                
His Lys Trp Val Val Leu Gly Arg Thr Arg Trp Gly Tyr Gly Asp Gly 
625                  630                635                  640
Leu Gly Gly Lys Glu Met Pro Phe Tyr Glu Asn Phe Tyr Ala Gly Gly 
                645                  650                655    
Ser Ser Thr Val Arg Gly Phe Gln Ser Asn Thr Ile Gly Pro Lys Ala 
            660                  665                670        
Val Tyr Phe Pro His Gln Ala Ser Asn Tyr Asp Pro Asp Tyr Asp Tyr 
        675                  680                685            
Glu Cys Ala Thr Gln Asp Gly Ala Lys Asp Leu Cys Lys Ser Asp Asp 
    690                  695                700                
Ala Val Gly Gly Asn Ala Met Ala Val Ala Ser Leu Glu Phe Ile Thr 
705                  710                715                  720
Pro Thr Pro Phe Ile Ser Asp Lys Tyr Ala Asn Ser Val Arg Thr Ser 
                725                  730                735    
Phe Phe Trp Asp Met Gly Thr Val Trp Asp Thr Asn Trp Asp Ser Ser 
            740                  745                750        
Gln Tyr Ser Gly Tyr Pro Asp Tyr Ser Asp Pro Ser Asn Ile Arg Met 
        755                  760                765            
Ser Ala Gly Ile Ala Leu Gln Trp Met Ser Pro Leu Gly Pro Leu Val 
    770                  775                780                
Phe Ser Tyr Ala Gln Pro Phe Lys Lys Tyr Asp Gly Asp Lys Ala Glu 
785                  790                795                  800
Gln Phe Gln Phe Asn Ile Gly Lys Thr Trp 
                805                  810

<210> 4
<211> 810
<212> PRT
<213> Escherichia coli

<220> 
<221> SOURCE
<222> 1..810
<223> /mol_type="protein"
      /note="Outer membrane protein assembly factor, Omp85 homologue"
      /organism="Escherichia coli"

<400> 4
Met Ala Met Lys Lys Leu Leu Ile Ala Ser Leu Leu Phe Ser Ser Ala 
1               5                   10                   15    
Thr Val Tyr Gly Ala Glu Gly Phe Val Val Lys Asp Ile His Phe Glu 
            20                   25                  30        
Gly Leu Gln Arg Val Ala Val Gly Ala Ala Leu Leu Ser Met Pro Val 
        35                   40                  45            
Arg Thr Gly Asp Thr Val Asn Asp Glu Asp Ile Ser Asn Thr Ile Arg 
    50                   55                  60                
Ala Leu Phe Ala Thr Gly Asn Phe Glu Asp Val Arg Val Leu Arg Asp 
65                   70                  75                  80
Gly Asp Thr Leu Leu Val Gln Val Lys Glu Arg Pro Thr Ile Ala Ser 
                85                   90                  95    
Ile Thr Phe Ser Gly Asn Lys Ser Val Lys Asp Asp Met Leu Lys Gln 
            100                  105                110        
Asn Leu Glu Ala Ser Gly Val Arg Val Gly Glu Ser Leu Asp Arg Thr 
        115                  120                125            
Thr Ile Ala Asp Ile Glu Lys Gly Leu Glu Asp Phe Tyr Tyr Ser Val 
    130                  135                140                
Gly Lys Tyr Ser Ala Ser Val Lys Ala Val Val Thr Pro Leu Pro Arg 
145                  150                155                  160
Asn Arg Val Asp Leu Lys Leu Val Phe Gln Glu Gly Val Ser Ala Glu 
                165                  170                175    
Ile Gln Gln Ile Asn Ile Val Gly Asn His Ala Phe Thr Thr Asp Glu 
            180                  185                190        
Leu Ile Ser His Phe Gln Leu Arg Asp Glu Val Pro Trp Trp Asn Val 
        195                  200                205            
Val Gly Asp Arg Lys Tyr Gln Lys Gln Lys Leu Ala Gly Asp Leu Glu 
    210                  215                220                
Thr Leu Arg Ser Tyr Tyr Leu Asp Arg Gly Tyr Ala Arg Phe Asn Ile 
225                  230                235                  240
Asp Ser Thr Gln Val Ser Leu Thr Pro Asp Lys Lys Gly Ile Tyr Val 
                245                  250                255    
Thr Val Asn Ile Thr Glu Gly Asp Gln Tyr Lys Leu Ser Gly Val Glu 
            260                  265                270        
Val Ser Gly Asn Leu Ala Gly His Ser Ala Glu Ile Glu Gln Leu Thr 
        275                  280                285            
Lys Ile Glu Pro Gly Glu Leu Tyr Asn Gly Thr Lys Val Thr Lys Met 
    290                  295                300                
Glu Asp Asp Ile Lys Lys Leu Leu Gly Arg Tyr Gly Tyr Ala Tyr Pro 
305                  310                315                  320
Arg Val Gln Ser Met Pro Glu Ile Asn Asp Ala Asp Lys Thr Val Lys 
                325                  330                335    
Leu Arg Val Asn Val Asp Ala Gly Asn Arg Phe Tyr Val Arg Lys Ile 
            340                  345                350        
Arg Phe Glu Gly Asn Asp Thr Ser Lys Asp Ala Val Leu Arg Arg Glu 
        355                  360                365            
Met Arg Gln Met Glu Gly Ala Trp Leu Gly Ser Asp Leu Val Asp Gln 
    370                  375                380                
Gly Lys Glu Arg Leu Asn Arg Leu Gly Phe Phe Glu Thr Val Asp Thr 
385                  390                395                  400
Asp Thr Gln Arg Val Pro Gly Ser Pro Asp Gln Val Asp Val Val Tyr 
                405                  410                415    
Lys Val Lys Glu Arg Asn Thr Gly Ser Phe Asn Phe Gly Ile Gly Tyr 
            420                  425                430        
Gly Thr Glu Ser Gly Val Ser Phe Gln Ala Gly Val Gln Gln Asp Asn 
        435                  440                445            
Trp Leu Gly Thr Gly Tyr Ala Val Gly Ile Asn Gly Thr Lys Asn Asp 
    450                  455                460                
Tyr Gln Thr Tyr Ala Glu Leu Ser Val Thr Asn Pro Tyr Phe Thr Val 
465                  470                475                  480
Asp Gly Val Ser Leu Gly Gly Arg Leu Phe Tyr Asn Asp Phe Gln Ala 
                485                  490                495    
Asp Asp Ala Asp Leu Ser Asp Tyr Thr Asn Lys Ser Tyr Gly Thr Asp 
            500                  505                510        
Val Thr Leu Gly Phe Pro Ile Asn Glu Tyr Asn Ser Leu Arg Ala Gly 
        515                  520                525            
Leu Gly Tyr Val His Asn Ser Leu Ser Asn Met Gln Pro Gln Val Ala 
    530                  535                540                
Met Trp Arg Tyr Leu Tyr Ser Met Gly Glu His Pro Ser Thr Ser Asp 
545                  550                555                  560
Gln Asp Asn Ser Phe Lys Thr Asp Asp Phe Thr Phe Asn Tyr Gly Trp 
                565                  570                575    
Thr Tyr Asn Lys Leu Asp Arg Gly Tyr Phe Pro Thr Asp Gly Ser Arg 
            580                  585                590        
Val Asn Leu Thr Gly Lys Val Thr Ile Pro Gly Ser Asp Asn Glu Tyr 
        595                  600                605            
Tyr Lys Val Thr Leu Asp Thr Ala Thr Tyr Val Pro Ile Asp Asp Asp 
    610                  615                620                
His Lys Trp Val Val Leu Gly Arg Thr Arg Trp Gly Tyr Gly Asp Gly 
625                  630                635                  640
Leu Gly Gly Lys Glu Met Pro Phe Tyr Glu Asn Phe Tyr Ala Gly Gly 
                645                  650                655    
Ser Ser Thr Val Arg Gly Phe Gln Ser Asn Thr Ile Gly Pro Lys Ala 
            660                  665                670        
Val Tyr Phe Pro His Gln Ala Ser Asn Tyr Asp Pro Asp Tyr Asp Tyr 
        675                  680                685            
Glu Cys Ala Thr Gln Asp Gly Ala Lys Asp Leu Cys Lys Ser Asp Asp 
    690                  695                700                
Ala Val Gly Gly Asn Ala Met Ala Val Ala Ser Leu Glu Phe Ile Thr 
705                  710                715                  720
Pro Thr Pro Phe Ile Ser Asp Lys Tyr Ala Asn Ser Val Arg Thr Ser 
                725                  730                735    
Phe Phe Trp Asp Met Gly Thr Val Trp Asp Thr Asn Trp Asp Ser Ser 
            740                  745                750        
Gln Tyr Ser Gly Tyr Pro Asp Tyr Ser Asp Pro Ser Asn Ile Arg Met 
        755                  760                765            
Ser Ala Gly Ile Ala Leu Gln Trp Met Ser Pro Leu Gly Pro Leu Val 
    770                  775                780                
Phe Ser Tyr Ala Gln Pro Phe Lys Lys Tyr Asp Gly Asp Lys Ala Glu 
785                  790                795                  800
Gln Phe Gln Phe Asn Ile Gly Lys Thr Trp 
                805                  810

<210> 5
<211> 1491
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1491
<223> /mol_type="DNA"
      /note="CYP3A4 in plasmid pSC001"
      /organism="Artificial Sequence"

<400> 5
ctcgagatgg ctctgttatt agcagttttt ctggtgctcc tctatctata tggaacccat      60

tcacatggac tttttaagaa gcttggaatt ccagggccca cacctctgcc ttttttggga     120

aatattttgt cctaccataa gggcttttgt atgtttgaca tggaatgtca taaaaagtat     180

ggaaaagtgt ggggctttta tgatggtcaa cagcctgtgc tggctatcac agatcctgac     240

atgatcaaaa cagtgctagt gaaagaatgt tattctgtct tcacaaaccg gaggcctttt     300

ggtccagtgg gatttatgaa aagtgccatc tctatagctg aggatgaaga atggaagaga     360

ttacgatcat tgctgtctcc aaccttcacc agtggaaaac tcaaggagat ggtccctatc     420

attgcccagt atggagatgt gttggtgaga aatctgaggc gggaagcaga gacaggcaag     480

cctgtcacct tgaaagacgt ctttggggcc tacagcatgg atgtgatcac tagcacatca     540

tttggagtga acatcgactc tctcaacaat ccacaagacc cctttgtgga aaacaccaag     600

aagcttttaa gatttgattt tttggatcca ttctttctct caataacagt ctttccattc     660

ctcatcccaa ttcttgaagt attaaatatc tgtgtgtttc caagagaagt tacaaatttt     720

ttaagaaaat ctgtaaaaag gatgaaagaa agtcgcctcg aagatacaca aaagcaccga     780

gtggatttcc ttcagctgat gattgactct cagaattcaa aagaaactga gtcccacaaa     840

gctctgtccg atctggagct cgtggcccaa tcaattatct ttatttttgc tggctatgaa     900

accacgagca gtgttctctc cttcattatg tatgaactgg ccactcaccc tgatgtccag     960

cagaaactgc aggaggaaat tgatgcagtt ttacccaata aggcaccacc cacctatgat    1020

actgtgctac agatggagta tcttgacatg gtggtgaatg aaacgctcag attattccca    1080

attgctatga gacttgagag ggtctgcaaa aaagatgttg agatcaatgg gatgttcatt    1140

cccaaagggg tggtggtgat gattccaagc tatgctcttc accgtgaccc aaagtactgg    1200

acagagcctg agaagttcct ccctgaaaga ttcagcaaga agaacaagga caacatagat    1260

ccttacatat acacaccctt tggaagtgga cccagaaact gcattggcat gaggtttgct    1320

ctcatgaaca tgaaacttgc tctaatcaga gtccttcaga acttctcctt caaaccttgt    1380

aaagaaacac agatccccct gaaattaagc ttaggaggac ttcttcaacc agaaaaaccc    1440

gttgttctaa aggttgagtc aagggatggc accgtaagtg gagccggtac c             1491


<210> 6
<211> 497
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..497
<223> /mol_type="protein"
      /note="Amino acid sequence of CYP3A4 in plasmid pSC001"
      /organism="Artificial Sequence"

<400> 6
Leu Glu Met Ala Leu Leu Leu Ala Val Phe Leu Val Leu Leu Tyr Leu 
1               5                   10                   15    
Tyr Gly Thr His Ser His Gly Leu Phe Lys Lys Leu Gly Ile Pro Gly 
            20                   25                  30        
Pro Thr Pro Leu Pro Phe Leu Gly Asn Ile Leu Ser Tyr His Lys Gly 
        35                   40                  45            
Phe Cys Met Phe Asp Met Glu Cys His Lys Lys Tyr Gly Lys Val Trp 
    50                   55                  60                
Gly Phe Tyr Asp Gly Gln Gln Pro Val Leu Ala Ile Thr Asp Pro Asp 
65                   70                  75                  80
Met Ile Lys Thr Val Leu Val Lys Glu Cys Tyr Ser Val Phe Thr Asn 
                85                   90                  95    
Arg Arg Pro Phe Gly Pro Val Gly Phe Met Lys Ser Ala Ile Ser Ile 
            100                  105                110        
Ala Glu Asp Glu Glu Trp Lys Arg Leu Arg Ser Leu Leu Ser Pro Thr 
        115                  120                125            
Phe Thr Ser Gly Lys Leu Lys Glu Met Val Pro Ile Ile Ala Gln Tyr 
    130                  135                140                
Gly Asp Val Leu Val Arg Asn Leu Arg Arg Glu Ala Glu Thr Gly Lys 
145                  150                155                  160
Pro Val Thr Leu Lys Asp Val Phe Gly Ala Tyr Ser Met Asp Val Ile 
                165                  170                175    
Thr Ser Thr Ser Phe Gly Val Asn Ile Asp Ser Leu Asn Asn Pro Gln 
            180                  185                190        
Asp Pro Phe Val Glu Asn Thr Lys Lys Leu Leu Arg Phe Asp Phe Leu 
        195                  200                205            
Asp Pro Phe Phe Leu Ser Ile Thr Val Phe Pro Phe Leu Ile Pro Ile 
    210                  215                220                
Leu Glu Val Leu Asn Ile Cys Val Phe Pro Arg Glu Val Thr Asn Phe 
225                  230                235                  240
Leu Arg Lys Ser Val Lys Arg Met Lys Glu Ser Arg Leu Glu Asp Thr 
                245                  250                255    
Gln Lys His Arg Val Asp Phe Leu Gln Leu Met Ile Asp Ser Gln Asn 
            260                  265                270        
Ser Lys Glu Thr Glu Ser His Lys Ala Leu Ser Asp Leu Glu Leu Val 
        275                  280                285            
Ala Gln Ser Ile Ile Phe Ile Phe Ala Gly Tyr Glu Thr Thr Ser Ser 
    290                  295                300                
Val Leu Ser Phe Ile Met Tyr Glu Leu Ala Thr His Pro Asp Val Gln 
305                  310                315                  320
Gln Lys Leu Gln Glu Glu Ile Asp Ala Val Leu Pro Asn Lys Ala Pro 
                325                  330                335    
Pro Thr Tyr Asp Thr Val Leu Gln Met Glu Tyr Leu Asp Met Val Val 
            340                  345                350        
Asn Glu Thr Leu Arg Leu Phe Pro Ile Ala Met Arg Leu Glu Arg Val 
        355                  360                365            
Cys Lys Lys Asp Val Glu Ile Asn Gly Met Phe Ile Pro Lys Gly Val 
    370                  375                380                
Val Val Met Ile Pro Ser Tyr Ala Leu His Arg Asp Pro Lys Tyr Trp 
385                  390                395                  400
Thr Glu Pro Glu Lys Phe Leu Pro Glu Arg Phe Ser Lys Lys Asn Lys 
                405                  410                415    
Asp Asn Ile Asp Pro Tyr Ile Tyr Thr Pro Phe Gly Ser Gly Pro Arg 
            420                  425                430        
Asn Cys Ile Gly Met Arg Phe Ala Leu Met Asn Met Lys Leu Ala Leu 
        435                  440                445            
Ile Arg Val Leu Gln Asn Phe Ser Phe Lys Pro Cys Lys Glu Thr Gln 
    450                  455                460                
Ile Pro Leu Lys Leu Ser Leu Gly Gly Leu Leu Gln Pro Glu Lys Pro 
465                  470                475                  480
Val Val Leu Lys Val Glu Ser Arg Asp Gly Thr Val Ser Gly Ala Gly 
                485                  490                495    
Thr 
    

<210> 7
<211> 1482
<212> DNA
<213> Escherichia coli

<220> 
<221> source
<222> 1..1482
<223> /mol_type="DNA"
      /note="tolC gene of E.coli"
      /organism="Escherichia coli"

<400> 7
atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc      60

caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt     120

aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta     180

ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac     240

ggcatcaact ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg     300

tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacgtat     360

cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat     420

gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat     480

caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc     540

gcacagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg     600

gtagagcagc tgcgccagat caccggtaac tactatccgg aactggctgc gctgaatgtc     660

gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa     720

cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc     780

caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac     840

acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga cgatagcaat     900

atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt     960

aactcgcagg tgaaacaggc acagtacaac tttgtcggtg ccagcgagca actggaaagt    1020

gcccatcgta gcgtcgtgca gaccgtgcgt tcctccttca acaacattaa tgcatctatc    1080

agtagcatta acgcctacaa acaagccgta gtttccgctc aaagctcatt agacgcgatg    1140

gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg    1200

ttgtacaacg ccaagcaaga gctggcgaat gcgcgttata actacctgat taatcagctg    1260

aatattaagt cagctctggg tacgttgaac gagcaggatc tgctggcact gaacaatgcg    1320

ctgagcaaac cggtttccac taatccggaa aacgttgcac cgcaaacgcc ggaacagaat    1380

gctattgctg atggttatgc gcctgatagc ccggcaccag tcgttcagca aacatccgca    1440

cgcactacca ccagtaacgg tcataaccct ttccgtaact ga                       1482


<210> 8
<211> 493
<212> PRT
<213> Escherichia coli

<220> 
<221> SOURCE
<222> 1..493
<223> /mol_type="protein"
      /note="Amino acid sequence of the tolC gene of E.coli"
      /organism="Escherichia coli"

<400> 8
Met Lys Lys Leu Leu Pro Ile Leu Ile Gly Leu Ser Leu Ser Gly Phe 
1               5                   10                   15    
Ser Ser Leu Ser Gln Ala Glu Asn Leu Met Gln Val Tyr Gln Gln Ala 
            20                   25                  30        
Arg Leu Ser Asn Pro Glu Leu Arg Lys Ser Ala Ala Asp Arg Asp Ala 
        35                   40                  45            
Ala Phe Glu Lys Ile Asn Glu Ala Arg Ser Pro Leu Leu Pro Gln Leu 
    50                   55                  60                
Gly Leu Gly Ala Asp Tyr Thr Tyr Ser Asn Gly Tyr Arg Asp Ala Asn 
65                   70                  75                  80
Gly Ile Asn Ser Asn Ala Thr Ser Ala Ser Leu Gln Leu Thr Gln Ser 
                85                   90                  95    
Ile Phe Asp Met Ser Lys Trp Arg Ala Leu Thr Leu Gln Glu Lys Ala 
            100                  105                110        
Ala Gly Ile Gln Asp Val Thr Tyr Gln Thr Asp Gln Gln Thr Leu Ile 
        115                  120                125            
Leu Asn Thr Ala Thr Ala Tyr Phe Asn Val Leu Asn Ala Ile Asp Val 
    130                  135                140                
Leu Ser Tyr Thr Gln Ala Gln Lys Glu Ala Ile Tyr Arg Gln Leu Asp 
145                  150                155                  160
Gln Thr Thr Gln Arg Phe Asn Val Gly Leu Val Ala Ile Thr Asp Val 
                165                  170                175    
Gln Asn Ala Arg Ala Gln Tyr Asp Thr Val Leu Ala Asn Glu Val Thr 
            180                  185                190        
Ala Arg Asn Asn Leu Asp Asn Ala Val Glu Gln Leu Arg Gln Ile Thr 
        195                  200                205            
Gly Asn Tyr Tyr Pro Glu Leu Ala Ala Leu Asn Val Glu Asn Phe Lys 
    210                  215                220                
Thr Asp Lys Pro Gln Pro Val Asn Ala Leu Leu Lys Glu Ala Glu Lys 
225                  230                235                  240
Arg Asn Leu Ser Leu Leu Gln Ala Arg Leu Ser Gln Asp Leu Ala Arg 
                245                  250                255    
Glu Gln Ile Arg Gln Ala Gln Asp Gly His Leu Pro Thr Leu Asp Leu 
            260                  265                270        
Thr Ala Ser Thr Gly Ile Ser Asp Thr Ser Tyr Ser Gly Ser Lys Thr 
        275                  280                285            
Arg Gly Ala Ala Gly Thr Gln Tyr Asp Asp Ser Asn Met Gly Gln Asn 
    290                  295                300                
Lys Val Gly Leu Ser Phe Ser Leu Pro Ile Tyr Gln Gly Gly Met Val 
305                  310                315                  320
Asn Ser Gln Val Lys Gln Ala Gln Tyr Asn Phe Val Gly Ala Ser Glu 
                325                  330                335    
Gln Leu Glu Ser Ala His Arg Ser Val Val Gln Thr Val Arg Ser Ser 
            340                  345                350        
Phe Asn Asn Ile Asn Ala Ser Ile Ser Ser Ile Asn Ala Tyr Lys Gln 
        355                  360                365            
Ala Val Val Ser Ala Gln Ser Ser Leu Asp Ala Met Glu Ala Gly Tyr 
    370                  375                380                
Ser Val Gly Thr Arg Thr Ile Val Asp Val Leu Asp Ala Thr Thr Thr 
385                  390                395                  400
Leu Tyr Asn Ala Lys Gln Glu Leu Ala Asn Ala Arg Tyr Asn Tyr Leu 
                405                  410                415    
Ile Asn Gln Leu Asn Ile Lys Ser Ala Leu Gly Thr Leu Asn Glu Gln 
            420                  425                430        
Asp Leu Leu Ala Leu Asn Asn Ala Leu Ser Lys Pro Val Ser Thr Asn 
        435                  440                445            
Pro Glu Asn Val Ala Pro Gln Thr Pro Glu Gln Asn Ala Ile Ala Asp 
    450                  455                460                
Gly Tyr Ala Pro Asp Ser Pro Ala Pro Val Val Gln Gln Thr Ser Ala 
465                  470                475                  480
Arg Thr Thr Thr Ser Asn Gly His Asn Pro Phe Arg Asn 
                485                  490            

<210> 9
<211> 93
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..93
<223> /mol_type="DNA"
      /note="Fragment of a typical  artificial autotransporter"
      /organism="Artificial Sequence"

<400> 9
ctatcttcag catatgcaca tggaacacct tctagactcg agagatcttg ccctgaatat     60

ttcaaaggtc caccttctcc acgatctctt aat                                  93


<210> 10
<211> 31
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..31
<223> /mol_type="protein"
      /note="Fragment of a typical  artificial autotransporter"
      /organism="Artificial Sequence"

<400> 10
Leu Ser Ser Ala Tyr Ala His Gly Thr Pro Ser Arg Leu Glu Arg Ser 
1               5                   10                   15    
Cys Pro Glu Tyr Phe Lys Gly Pro Pro Ser Pro Arg Ser Leu Asn 
            20                   25                  30    

<210> 11
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="Fragment with important restriction site "
      /organism="Artificial Sequence"

<400> 11
actgatttgc tcgagatgga agaa                                           24


<210> 12
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..8
<223> /mol_type="protein"
      /note="Fragment with important restriction site"
      /organism="Artificial Sequence"

<400> 12
Thr Asp Leu Leu Glu Met Glu Glu 
1               5            

<210> 13
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..24
<223> /mol_type="DNA"
      /note="Fragment with important restriction site"
      /organism="Artificial Sequence"

<400> 13
agccgcatgg gtacccttaa tcct                                           24


<210> 14
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<221> SOURCE
<222> 1..8
<223> /mol_type="protein"
      /note="Fragment with important restriction site"
      /organism="Artificial Sequence"

<400> 14
Ser Arg Met Gly Thr Leu Asn Pro 
1               5            

