                         SEQUENCE LISTING

<110>  RiboxX GmbH
 
<120>  Microwave-driven RNA polymerization by RNA polymerases of 
       caliciviruses

<130>  R 0076 WO

<150>  EP 10 178 114.4
<151>  2010-09-21

<160>  17    

<170>  PatentIn version 3.3

<210>  1
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  A-motif


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Xaa = any amino acid

<400>  1

Xaa Xaa Asp Tyr Ser 
1               5   


<210>  2
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  B-motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa = any amino acid

<400>  2

Gly Xaa Pro Ser Gly 
1               5   


<210>  3
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  C-motif

<400>  3

Tyr Gly Asp Asp 
1               


<210>  4
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  D-motif


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Xaa = any amino acid

<400>  4

Xaa Xaa Tyr Gly Leu 
1               5   


<210>  5
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  E-Motif


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  Xaa = any amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa = any amino acid

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Xaa = any amino acid

<400>  5

Xaa Xaa Xaa Xaa Phe Leu Xaa Arg Xaa Xaa 
1               5                   10  


<210>  6
<211>  520
<212>  PRT
<213>  Norovirus

<400>  6

Met Gly Gly Asp Ser Lys Gly Thr Tyr Cys Gly Ala Pro Ile Leu Gly 
1               5                   10                  15      


Pro Gly Ser Ala Pro Lys Leu Ser Thr Lys Thr Lys Phe Trp Arg Ser 
            20                  25                  30          


Ser Thr Thr Pro Leu Pro Pro Gly Thr Tyr Glu Pro Ala Tyr Leu Gly 
        35                  40                  45              


Gly Lys Asp Pro Arg Val Lys Gly Gly Pro Ser Leu Gln Gln Val Met 
    50                  55                  60                  


Arg Asp Gln Leu Lys Pro Phe Thr Glu Pro Arg Gly Lys Pro Pro Lys 
65                  70                  75                  80  


Pro Ser Val Leu Glu Ala Ala Lys Lys Thr Ile Ile Asn Val Leu Glu 
                85                  90                  95      


Gln Thr Ile Asp Pro Pro Glu Lys Trp Ser Phe Thr Gln Ala Cys Ala 
            100                 105                 110         


Ser Leu Asp Lys Thr Thr Ser Ser Gly His Pro His His Met Arg Lys 
        115                 120                 125             


Asn Asp Cys Trp Asn Gly Glu Ser Phe Thr Gly Lys Leu Ala Asp Gln 
    130                 135                 140                 


Ala Ser Lys Ala Asn Leu Met Phe Glu Gly Gly Lys Asn Met Thr Pro 
145                 150                 155                 160 


Val Tyr Thr Gly Ala Leu Lys Asp Glu Leu Val Lys Thr Asp Lys Ile 
                165                 170                 175     


Tyr Gly Lys Ile Lys Lys Arg Leu Leu Trp Gly Ser Asp Leu Ala Thr 
            180                 185                 190         


Met Ile Arg Cys Ala Arg Ala Phe Gly Gly Leu Met Asp Glu Leu Lys 
        195                 200                 205             


Ala His Cys Val Thr Leu Pro Ile Arg Val Gly Met Asn Met Asn Glu 
    210                 215                 220                 


Asp Gly Pro Ile Ile Phe Glu Arg His Ser Arg Tyr Lys Tyr His Tyr 
225                 230                 235                 240 


Asp Ala Asp Tyr Ser Arg Trp Asp Ser Thr Gln Gln Arg Ala Val Leu 
                245                 250                 255     


Ala Ala Ala Leu Glu Ile Met Val Lys Phe Ser Ser Glu Pro His Leu 
            260                 265                 270         


Ala Gln Val Val Ala Glu Asp Leu Leu Ser Pro Ser Val Val Asp Val 
        275                 280                 285             


Gly Asp Phe Lys Ile Ser Ile Asn Glu Gly Leu Pro Ser Gly Val Pro 
    290                 295                 300                 


Cys Thr Ser Gln Trp Asn Ser Ile Ala His Trp Leu Leu Thr Leu Cys 
305                 310                 315                 320 


Ala Leu Ser Glu Val Thr Asn Leu Ser Pro Asp Ile Ile Gln Ala Asn 
                325                 330                 335     


Ser Leu Phe Ser Phe Tyr Gly Asp Asp Glu Ile Val Ser Thr Asp Ile 
            340                 345                 350         


Lys Leu Asp Pro Glu Lys Leu Thr Ala Lys Leu Lys Glu Tyr Gly Leu 
        355                 360                 365             


Lys Pro Thr Arg Pro Asp Lys Thr Glu Gly Pro Leu Val Ile Ser Glu 
    370                 375                 380                 


Asp Leu Asn Gly Leu Thr Phe Leu Arg Arg Thr Val Thr Arg Asp Pro 
385                 390                 395                 400 


Ala Gly Trp Phe Gly Lys Leu Glu Gln Ser Ser Ile Leu Arg Gln Met 
                405                 410                 415     


Tyr Trp Thr Arg Gly Pro Asn His Glu Asp Pro Ser Glu Thr Met Ile 
            420                 425                 430         


Pro His Ser Gln Arg Pro Ile Gln Leu Met Ser Leu Leu Gly Glu Ala 
        435                 440                 445             


Ala Leu His Gly Pro Ala Phe Tyr Ser Lys Ile Ser Lys Leu Val Ile 
    450                 455                 460                 


Ala Glu Leu Lys Glu Gly Gly Met Asp Phe Tyr Val Pro Arg Gln Glu 
465                 470                 475                 480 


Pro Met Phe Arg Trp Met Arg Phe Ser Asp Leu Ser Thr Trp Glu Gly 
                485                 490                 495     


Asp Arg Asn Leu Ala Pro Ser Phe Val Asn Glu Asp Gly Val Glu Val 
            500                 505                 510         


Asp Lys Leu Ala Ala Ala Leu Glu 
        515                 520 


<210>  7
<211>  517
<212>  PRT
<213>  Sapovirus

<400>  7

Met Lys Asp Glu Phe Gln Trp Lys Gly Leu Pro Val Val Lys Ser Gly 
1               5                   10                  15      


Leu Asp Val Gly Gly Met Pro Thr Gly Thr Arg Tyr His Arg Ser Pro 
            20                  25                  30          


Ala Trp Pro Glu Glu Gln Pro Gly Glu Thr His Ala Pro Ala Pro Phe 
        35                  40                  45              


Gly Ala Gly Asp Lys Arg Tyr Thr Phe Ser Gln Thr Glu Met Leu Val 
    50                  55                  60                  


Asn Gly Leu Lys Pro Tyr Thr Glu Pro Thr Ala Gly Val Pro Pro Gln 
65                  70                  75                  80  


Leu Leu Ser Arg Ala Val Thr His Val Arg Ser Tyr Ile Glu Thr Ile 
                85                  90                  95      


Ile Gly Thr His Arg Ser Pro Val Leu Thr Tyr His Gln Ala Cys Glu 
            100                 105                 110         


Leu Leu Glu Arg Thr Thr Ser Cys Gly Pro Phe Val Gln Gly Leu Lys 
        115                 120                 125             


Gly Asp Tyr Trp Asp Glu Glu Gln Gln Gln Tyr Thr Gly Val Leu Ala 
    130                 135                 140                 


Asn His Leu Glu Gln Ala Trp Asp Lys Ala Asn Lys Gly Ile Ala Pro 
145                 150                 155                 160 


Arg Asn Ala Tyr Lys Leu Ala Leu Lys Asp Glu Leu Arg Pro Ile Glu 
                165                 170                 175     


Lys Asn Lys Ala Gly Lys Arg Arg Leu Leu Trp Gly Cys Asp Ala Ala 
            180                 185                 190         


Thr Thr Leu Ile Ala Thr Ala Ala Phe Lys Ala Val Ala Thr Arg Leu 
        195                 200                 205             


Gln Val Val Thr Pro Met Thr Pro Val Ala Val Gly Ile Asn Met Asp 
    210                 215                 220                 


Ser Val Gln Met Gln Val Met Asn Asp Ser Leu Lys Gly Gly Val Leu 
225                 230                 235                 240 


Tyr Cys Leu Asp Tyr Ser Lys Trp Asp Ser Thr Gln Asn Pro Ala Val 
                245                 250                 255     


Thr Ala Ala Ser Leu Ala Ile Leu Glu Arg Phe Ala Glu Pro His Pro 
            260                 265                 270         


Ile Val Ser Cys Ala Ile Glu Ala Leu Ser Ser Pro Ala Glu Gly Tyr 
        275                 280                 285             


Val Asn Asp Ile Lys Phe Val Thr Arg Gly Gly Leu Pro Ser Gly Met 
    290                 295                 300                 


Pro Phe Thr Ser Val Val Asn Ser Ile Asn His Met Ile Tyr Val Ala 
305                 310                 315                 320 


Ala Ala Ile Leu Gln Ala Tyr Glu Ser His Asn Val Pro Tyr Thr Gly 
                325                 330                 335     


Asn Val Phe Gln Val Glu Thr Val His Thr Tyr Gly Asp Asp Cys Met 
            340                 345                 350         


Tyr Ser Val Cys Pro Ala Thr Ala Ser Ile Phe His Ala Val Leu Ala 
        355                 360                 365             


Asn Leu Thr Ser Tyr Gly Leu Lys Pro Thr Ala Ala Asp Lys Ser Asp 
    370                 375                 380                 


Ala Ile Lys Pro Thr Asn Thr Pro Val Phe Leu Lys Arg Thr Phe Thr 
385                 390                 395                 400 


Gln Thr Pro His Gly Val Arg Ala Leu Leu Asp Ile Thr Ser Ile Thr 
                405                 410                 415     


Arg Gln Phe Tyr Trp Leu Lys Ala Asn Arg Thr Ser Asp Pro Ser Ser 
            420                 425                 430         


Pro Pro Ala Phe Asp Arg Gln Ala Arg Ser Ala Gln Leu Glu Asn Ala 
        435                 440                 445             


Leu Ala Tyr Ala Ser Gln His Gly Pro Val Val Phe Asp Thr Val Arg 
    450                 455                 460                 


Gln Ile Ala Ile Lys Thr Ala Gln Gly Glu Gly Leu Val Leu Val Asn 
465                 470                 475                 480 


Thr Asn Tyr Asp Gln Ala Leu Ala Thr Tyr Asn Ala Trp Phe Ile Gly 
                485                 490                 495     


Gly Thr Val Pro Asp Pro Val Gly His Thr Glu Gly Thr His Lys Ile 
            500                 505                 510         


Val Phe Glu Met Glu 
        515         


<210>  8
<211>  533
<212>  PRT
<213>  Vesivirus

<400>  8

Met Lys Val Thr Thr Gln Lys Tyr Asp Val Thr Lys Pro Asp Ile Ser 
1               5                   10                  15      


Tyr Lys Gly Leu Ile Cys Lys Gln Leu Asp Glu Ile Arg Val Ile Pro 
            20                  25                  30          


Lys Gly Thr Arg Leu His Val Ser Pro Ala His Thr Asp Asp Tyr Asp 
        35                  40                  45              


Glu Cys Ser His Gln Pro Ala Ser Leu Gly Ser Gly Asp Pro Arg Cys 
    50                  55                  60                  


Pro Lys Ser Leu Thr Ala Ile Val Val Asp Ser Leu Lys Pro Tyr Cys 
65                  70                  75                  80  


Glu Lys Thr Asp Gly Pro Pro His Asp Ile Leu His Arg Val Gln Arg 
                85                  90                  95      


Met Leu Ile Asp His Leu Ser Gly Phe Val Pro Met Asn Ile Ser Ser 
            100                 105                 110         


Glu Pro Ser Met Leu Ala Ala Phe His Lys Leu Asn His Asp Thr Ser 
        115                 120                 125             


Cys Gly Pro Tyr Leu Gly Gly Arg Lys Lys Asp His Met Ile Gly Gly 
    130                 135                 140                 


Glu Pro Asp Lys Pro Leu Leu Asp Leu Leu Ser Ser Lys Trp Lys Leu 
145                 150                 155                 160 


Ala Thr Gln Gly Ile Gly Leu Pro His Glu Tyr Thr Ile Gly Leu Lys 
                165                 170                 175     


Asp Glu Leu Arg Pro Val Glu Lys Val Gln Glu Gly Lys Arg Arg Met 
            180                 185                 190         


Ile Trp Gly Cys Asp Val Gly Val Ala Thr Val Cys Ala Ala Ala Phe 
        195                 200                 205             


Lys Gly Val Ser Asp Ala Ile Thr Ala Asn His Gln Tyr Gly Pro Val 
    210                 215                 220                 


Gln Val Gly Ile Asn Met Asp Gly Pro Ser Val Glu Ala Leu Tyr Gln 
225                 230                 235                 240 


Arg Ile Arg Ser Ala Ala Lys Val Phe Ala Val Asp Tyr Ser Lys Trp 
                245                 250                 255     


Asp Ser Thr Gln Ser Pro Arg Val Ser Ala Ala Ser Ile Asp Ile Leu 
            260                 265                 270         


Arg Tyr Phe Ser Asp Arg Ser Pro Ile Val Asp Ser Ala Ala Asn Thr 
        275                 280                 285             


Leu Lys Ser Pro Pro Ile Ala Ile Phe Asn Gly Val Ala Val Lys Val 
    290                 295                 300                 


Thr Ser Gly Leu Pro Ser Gly Met Pro Leu Thr Ser Val Ile Asn Ser 
305                 310                 315                 320 


Leu Asn His Cys Leu Tyr Val Gly Cys Ala Ile Leu Gln Ser Leu Glu 
                325                 330                 335     


Ser Arg Asn Ile Pro Val Thr Trp Asn Leu Phe Ser Thr Phe Asp Met 
            340                 345                 350         


Met Thr Tyr Gly Asp Asp Gly Val Tyr Met Phe Pro Met Met Phe Ala 
        355                 360                 365             


Ser Val Ser Asp Gln Ile Phe Ala Asn Leu Thr Ala Tyr Gly Leu Lys 
    370                 375                 380                 


Pro Thr Arg Val Asp Lys Ser Val Gly Ala Ile Glu Pro Ile Asp Pro 
385                 390                 395                 400 


Glu Ser Val Val Phe Leu Lys Arg Thr Ile Thr Arg Thr Pro His Gly 
                405                 410                 415     


Ile Arg Gly Leu Leu Asp Arg Gly Ser Ile Ile Arg Gln Phe Tyr Tyr 
            420                 425                 430         


Ile Lys Gly Glu Asn Ser Asp Asp Trp Lys Thr Pro Pro Lys Thr Ile 
        435                 440                 445             


Asp Pro Thr Ser Arg Gly Gln Gln Leu Trp Asn Ala Cys Leu Tyr Ala 
    450                 455                 460                 


Ser Gln His Gly Pro Glu Phe Tyr Asn Lys Val Tyr Arg Leu Ala Glu 
465                 470                 475                 480 


Lys Ala Val Glu Tyr Glu Glu Leu His Phe Glu Pro Pro Ser Tyr His 
                485                 490                 495     


Ser Ala Leu Glu His Tyr Asn Asn Gln Phe Asn Gly Val Asp Thr Arg 
            500                 505                 510         


Ser Asp Gln Ile Asp Ala Ser Val Met Thr Asp Leu His Cys Asp Val 
        515                 520                 525             


Phe Glu Val Leu Glu 
    530             


<210>  9
<211>  517
<212>  PRT
<213>  Lagovirus

<400>  9

Met Thr Ser Asn Phe Phe Cys Gly Glu Pro Ile Asp Tyr Arg Gly Ile 
1               5                   10                  15      


Thr Ala His Arg Leu Val Gly Ala Glu Pro Arg Pro Pro Val Ser Gly 
            20                  25                  30          


Thr Arg Tyr Ala Lys Val Pro Gly Val Pro Asp Glu Tyr Lys Thr Gly 
        35                  40                  45              


Tyr Arg Pro Ala Asn Leu Gly Arg Ser Asp Pro Asp Ser Asp Lys Ser 
    50                  55                  60                  


Leu Met Asn Ile Ala Val Lys Asn Leu Gln Val Tyr Gln Gln Glu Pro 
65                  70                  75                  80  


Lys Leu Asp Lys Val Asp Glu Phe Ile Glu Arg Ala Ala Ala Asp Val 
                85                  90                  95      


Leu Gly Tyr Leu Arg Phe Leu Thr Lys Gly Glu Arg Gln Ala Asn Leu 
            100                 105                 110         


Asn Phe Lys Ala Ala Phe Asn Thr Leu Asp Leu Ser Thr Ser Cys Gly 
        115                 120                 125             


Pro Phe Val Pro Gly Lys Lys Ile Asp His Val Lys Asp Gly Val Met 
    130                 135                 140                 


Asp Gln Val Leu Ala Lys His Leu Tyr Lys Cys Trp Ser Val Ala Asn 
145                 150                 155                 160 


Ser Gly Lys Ala Leu His His Ile Tyr Ala Cys Gly Leu Lys Asp Glu 
                165                 170                 175     


Leu Arg Pro Leu Asp Lys Val Lys Glu Gly Lys Lys Arg Leu Leu Trp 
            180                 185                 190         


Gly Cys Asp Val Gly Val Ala Val Cys Ala Ala Ala Val Phe His Asn 
        195                 200                 205             


Ile Cys Tyr Lys Leu Lys Met Val Ala Arg Phe Gly Pro Ile Ala Val 
    210                 215                 220                 


Gly Val Asp Met Thr Ser Arg Asp Val Asp Val Ile Ile Asn Asn Leu 
225                 230                 235                 240 


Thr Ser Lys Ala Ser Asp Phe Leu Cys Leu Asp Tyr Ser Lys Trp Asp 
                245                 250                 255     


Ser Thr Met Ser Pro Cys Val Val Arg Leu Ala Ile Asp Ile Leu Ala 
            260                 265                 270         


Asp Cys Cys Glu Gln Thr Glu Leu Thr Lys Ser Val Val Leu Thr Leu 
        275                 280                 285             


Lys Ser His Pro Met Thr Ile Leu Asp Ala Met Ile Val Gln Thr Lys 
    290                 295                 300                 


Arg Gly Leu Pro Ser Gly Met Pro Phe Thr Ser Val Ile Asn Ser Ile 
305                 310                 315                 320 


Cys His Trp Leu Leu Trp Ser Ala Ala Val Tyr Lys Ser Cys Ala Glu 
                325                 330                 335     


Ile Gly Leu His Cys Ser Asn Leu Tyr Glu Asp Ala Pro Phe Tyr Thr 
            340                 345                 350         


Tyr Gly Asp Asp Gly Val Tyr Ala Met Thr Pro Met Met Val Ser Leu 
        355                 360                 365             


Leu Pro Ala Ile Ile Glu Asn Leu Arg Asp Tyr Gly Leu Ser Pro Thr 
    370                 375                 380                 


Ala Ala Asp Lys Thr Glu Phe Ile Asp Val Cys Pro Leu Asn Lys Ile 
385                 390                 395                 400 


Ser Phe Leu Lys Arg Thr Phe Glu Leu Thr Asp Ile Gly Trp Val Ser 
                405                 410                 415     


Lys Leu Asp Lys Ser Ser Ile Leu Arg Gln Leu Glu Trp Ser Lys Thr 
            420                 425                 430         


Thr Ser Arg His Met Val Ile Glu Glu Thr Tyr Asp Leu Ala Lys Glu 
        435                 440                 445             


Glu Arg Gly Val Gln Leu Glu Glu Leu Gln Val Ala Ala Ala Ala His 
    450                 455                 460                 


Gly Gln Glu Phe Phe Asn Phe Val Cys Arg Glu Leu Glu Arg Gln Gln 
465                 470                 475                 480 


Ala Tyr Thr Gln Phe Ser Val Tyr Ser Tyr Asp Ala Ala Arg Lys Ile 
                485                 490                 495     


Leu Ala Asp Arg Lys Arg Val Val Ser Val Val Pro Asp Asp Glu Phe 
            500                 505                 510         


Val Asn Val Met Glu 
        515         


<210>  10
<211>  526
<212>  PRT
<213>  Artificial

<220>
<223>  Norovirus RNA polymerase containing His tag

<400>  10

Met Gly Gly Asp Ser Lys Gly Thr Tyr Cys Gly Ala Pro Ile Leu Gly 
1               5                   10                  15      


Pro Gly Ser Ala Pro Lys Leu Ser Thr Lys Thr Lys Phe Trp Arg Ser 
            20                  25                  30          


Ser Thr Thr Pro Leu Pro Pro Gly Thr Tyr Glu Pro Ala Tyr Leu Gly 
        35                  40                  45              


Gly Lys Asp Pro Arg Val Lys Gly Gly Pro Ser Leu Gln Gln Val Met 
    50                  55                  60                  


Arg Asp Gln Leu Lys Pro Phe Thr Glu Pro Arg Gly Lys Pro Pro Lys 
65                  70                  75                  80  


Pro Ser Val Leu Glu Ala Ala Lys Lys Thr Ile Ile Asn Val Leu Glu 
                85                  90                  95      


Gln Thr Ile Asp Pro Pro Glu Lys Trp Ser Phe Thr Gln Ala Cys Ala 
            100                 105                 110         


Ser Leu Asp Lys Thr Thr Ser Ser Gly His Pro His His Met Arg Lys 
        115                 120                 125             


Asn Asp Cys Trp Asn Gly Glu Ser Phe Thr Gly Lys Leu Ala Asp Gln 
    130                 135                 140                 


Ala Ser Lys Ala Asn Leu Met Phe Glu Gly Gly Lys Asn Met Thr Pro 
145                 150                 155                 160 


Val Tyr Thr Gly Ala Leu Lys Asp Glu Leu Val Lys Thr Asp Lys Ile 
                165                 170                 175     


Tyr Gly Lys Ile Lys Lys Arg Leu Leu Trp Gly Ser Asp Leu Ala Thr 
            180                 185                 190         


Met Ile Arg Cys Ala Arg Ala Phe Gly Gly Leu Met Asp Glu Leu Lys 
        195                 200                 205             


Ala His Cys Val Thr Leu Pro Ile Arg Val Gly Met Asn Met Asn Glu 
    210                 215                 220                 


Asp Gly Pro Ile Ile Phe Glu Arg His Ser Arg Tyr Lys Tyr His Tyr 
225                 230                 235                 240 


Asp Ala Asp Tyr Ser Arg Trp Asp Ser Thr Gln Gln Arg Ala Val Leu 
                245                 250                 255     


Ala Ala Ala Leu Glu Ile Met Val Lys Phe Ser Ser Glu Pro His Leu 
            260                 265                 270         


Ala Gln Val Val Ala Glu Asp Leu Leu Ser Pro Ser Val Val Asp Val 
        275                 280                 285             


Gly Asp Phe Lys Ile Ser Ile Asn Glu Gly Leu Pro Ser Gly Val Pro 
    290                 295                 300                 


Cys Thr Ser Gln Trp Asn Ser Ile Ala His Trp Leu Leu Thr Leu Cys 
305                 310                 315                 320 


Ala Leu Ser Glu Val Thr Asn Leu Ser Pro Asp Ile Ile Gln Ala Asn 
                325                 330                 335     


Ser Leu Phe Ser Phe Tyr Gly Asp Asp Glu Ile Val Ser Thr Asp Ile 
            340                 345                 350         


Lys Leu Asp Pro Glu Lys Leu Thr Ala Lys Leu Lys Glu Tyr Gly Leu 
        355                 360                 365             


Lys Pro Thr Arg Pro Asp Lys Thr Glu Gly Pro Leu Val Ile Ser Glu 
    370                 375                 380                 


Asp Leu Asn Gly Leu Thr Phe Leu Arg Arg Thr Val Thr Arg Asp Pro 
385                 390                 395                 400 


Ala Gly Trp Phe Gly Lys Leu Glu Gln Ser Ser Ile Leu Arg Gln Met 
                405                 410                 415     


Tyr Trp Thr Arg Gly Pro Asn His Glu Asp Pro Ser Glu Thr Met Ile 
            420                 425                 430         


Pro His Ser Gln Arg Pro Ile Gln Leu Met Ser Leu Leu Gly Glu Ala 
        435                 440                 445             


Ala Leu His Gly Pro Ala Phe Tyr Ser Lys Ile Ser Lys Leu Val Ile 
    450                 455                 460                 


Ala Glu Leu Lys Glu Gly Gly Met Asp Phe Tyr Val Pro Arg Gln Glu 
465                 470                 475                 480 


Pro Met Phe Arg Trp Met Arg Phe Ser Asp Leu Ser Thr Trp Glu Gly 
                485                 490                 495     


Asp Arg Asn Leu Ala Pro Ser Phe Val Asn Glu Asp Gly Val Glu Val 
            500                 505                 510         


Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His 
        515                 520                 525     


<210>  11
<211>  523
<212>  PRT
<213>  Artificial

<220>
<223>  Sapovirus RNA polymerase containing His tag

<400>  11

Met Lys His His His His His His Asp Glu Phe Gln Trp Lys Gly Leu 
1               5                   10                  15      


Pro Val Val Lys Ser Gly Leu Asp Val Gly Gly Met Pro Thr Gly Thr 
            20                  25                  30          


Arg Tyr His Arg Ser Pro Ala Trp Pro Glu Glu Gln Pro Gly Glu Thr 
        35                  40                  45              


His Ala Pro Ala Pro Phe Gly Ala Gly Asp Lys Arg Tyr Thr Phe Ser 
    50                  55                  60                  


Gln Thr Glu Met Leu Val Asn Gly Leu Lys Pro Tyr Thr Glu Pro Thr 
65                  70                  75                  80  


Ala Gly Val Pro Pro Gln Leu Leu Ser Arg Ala Val Thr His Val Arg 
                85                  90                  95      


Ser Tyr Ile Glu Thr Ile Ile Gly Thr His Arg Ser Pro Val Leu Thr 
            100                 105                 110         


Tyr His Gln Ala Cys Glu Leu Leu Glu Arg Thr Thr Ser Cys Gly Pro 
        115                 120                 125             


Phe Val Gln Gly Leu Lys Gly Asp Tyr Trp Asp Glu Glu Gln Gln Gln 
    130                 135                 140                 


Tyr Thr Gly Val Leu Ala Asn His Leu Glu Gln Ala Trp Asp Lys Ala 
145                 150                 155                 160 


Asn Lys Gly Ile Ala Pro Arg Asn Ala Tyr Lys Leu Ala Leu Lys Asp 
                165                 170                 175     


Glu Leu Arg Pro Ile Glu Lys Asn Lys Ala Gly Lys Arg Arg Leu Leu 
            180                 185                 190         


Trp Gly Cys Asp Ala Ala Thr Thr Leu Ile Ala Thr Ala Ala Phe Lys 
        195                 200                 205             


Ala Val Ala Thr Arg Leu Gln Val Val Thr Pro Met Thr Pro Val Ala 
    210                 215                 220                 


Val Gly Ile Asn Met Asp Ser Val Gln Met Gln Val Met Asn Asp Ser 
225                 230                 235                 240 


Leu Lys Gly Gly Val Leu Tyr Cys Leu Asp Tyr Ser Lys Trp Asp Ser 
                245                 250                 255     


Thr Gln Asn Pro Ala Val Thr Ala Ala Ser Leu Ala Ile Leu Glu Arg 
            260                 265                 270         


Phe Ala Glu Pro His Pro Ile Val Ser Cys Ala Ile Glu Ala Leu Ser 
        275                 280                 285             


Ser Pro Ala Glu Gly Tyr Val Asn Asp Ile Lys Phe Val Thr Arg Gly 
    290                 295                 300                 


Gly Leu Pro Ser Gly Met Pro Phe Thr Ser Val Val Asn Ser Ile Asn 
305                 310                 315                 320 


His Met Ile Tyr Val Ala Ala Ala Ile Leu Gln Ala Tyr Glu Ser His 
                325                 330                 335     


Asn Val Pro Tyr Thr Gly Asn Val Phe Gln Val Glu Thr Val His Thr 
            340                 345                 350         


Tyr Gly Asp Asp Cys Met Tyr Ser Val Cys Pro Ala Thr Ala Ser Ile 
        355                 360                 365             


Phe His Ala Val Leu Ala Asn Leu Thr Ser Tyr Gly Leu Lys Pro Thr 
    370                 375                 380                 


Ala Ala Asp Lys Ser Asp Ala Ile Lys Pro Thr Asn Thr Pro Val Phe 
385                 390                 395                 400 


Leu Lys Arg Thr Phe Thr Gln Thr Pro His Gly Val Arg Ala Leu Leu 
                405                 410                 415     


Asp Ile Thr Ser Ile Thr Arg Gln Phe Tyr Trp Leu Lys Ala Asn Arg 
            420                 425                 430         


Thr Ser Asp Pro Ser Ser Pro Pro Ala Phe Asp Arg Gln Ala Arg Ser 
        435                 440                 445             


Ala Gln Leu Glu Asn Ala Leu Ala Tyr Ala Ser Gln His Gly Pro Val 
    450                 455                 460                 


Val Phe Asp Thr Val Arg Gln Ile Ala Ile Lys Thr Ala Gln Gly Glu 
465                 470                 475                 480 


Gly Leu Val Leu Val Asn Thr Asn Tyr Asp Gln Ala Leu Ala Thr Tyr 
                485                 490                 495     


Asn Ala Trp Phe Ile Gly Gly Thr Val Pro Asp Pro Val Gly His Thr 
            500                 505                 510         


Glu Gly Thr His Lys Ile Val Phe Glu Met Glu 
        515                 520             


<210>  12
<211>  523
<212>  PRT
<213>  Artificial

<220>
<223>  Sapovirus RNA polymerase containing His tag

<400>  12

Met Lys Asp Glu Phe Gln Trp Lys Gly Leu Pro Val Val Lys Ser Gly 
1               5                   10                  15      


Leu Asp Val Gly Gly Met Pro Thr Gly Thr Arg Tyr His Arg Ser Pro 
            20                  25                  30          


Ala Trp Pro Glu Glu Gln Pro Gly Glu Thr His Ala Pro Ala Pro Phe 
        35                  40                  45              


Gly Ala Gly Asp Lys Arg Tyr Thr Phe Ser Gln Thr Glu Met Leu Val 
    50                  55                  60                  


Asn Gly Leu Lys Pro Tyr Thr Glu Pro Thr Ala Gly Val Pro Pro Gln 
65                  70                  75                  80  


Leu Leu Ser Arg Ala Val Thr His Val Arg Ser Tyr Ile Glu Thr Ile 
                85                  90                  95      


Ile Gly Thr His Arg Ser Pro Val Leu Thr Tyr His Gln Ala Cys Glu 
            100                 105                 110         


Leu Leu Glu Arg Thr Thr Ser Cys Gly Pro Phe Val Gln Gly Leu Lys 
        115                 120                 125             


Gly Asp Tyr Trp Asp Glu Glu Gln Gln Gln Tyr Thr Gly Val Leu Ala 
    130                 135                 140                 


Asn His Leu Glu Gln Ala Trp Asp Lys Ala Asn Lys Gly Ile Ala Pro 
145                 150                 155                 160 


Arg Asn Ala Tyr Lys Leu Ala Leu Lys Asp Glu Leu Arg Pro Ile Glu 
                165                 170                 175     


Lys Asn Lys Ala Gly Lys Arg Arg Leu Leu Trp Gly Cys Asp Ala Ala 
            180                 185                 190         


Thr Thr Leu Ile Ala Thr Ala Ala Phe Lys Ala Val Ala Thr Arg Leu 
        195                 200                 205             


Gln Val Val Thr Pro Met Thr Pro Val Ala Val Gly Ile Asn Met Asp 
    210                 215                 220                 


Ser Val Gln Met Gln Val Met Asn Asp Ser Leu Lys Gly Gly Val Leu 
225                 230                 235                 240 


Tyr Cys Leu Asp Tyr Ser Lys Trp Asp Ser Thr Gln Asn Pro Ala Val 
                245                 250                 255     


Thr Ala Ala Ser Leu Ala Ile Leu Glu Arg Phe Ala Glu Pro His Pro 
            260                 265                 270         


Ile Val Ser Cys Ala Ile Glu Ala Leu Ser Ser Pro Ala Glu Gly Tyr 
        275                 280                 285             


Val Asn Asp Ile Lys Phe Val Thr Arg Gly Gly Leu Pro Ser Gly Met 
    290                 295                 300                 


Pro Phe Thr Ser Val Val Asn Ser Ile Asn His Met Ile Tyr Val Ala 
305                 310                 315                 320 


Ala Ala Ile Leu Gln Ala Tyr Glu Ser His Asn Val Pro Tyr Thr Gly 
                325                 330                 335     


Asn Val Phe Gln Val Glu Thr Val His Thr Tyr Gly Asp Asp Cys Met 
            340                 345                 350         


Tyr Ser Val Cys Pro Ala Thr Ala Ser Ile Phe His Ala Val Leu Ala 
        355                 360                 365             


Asn Leu Thr Ser Tyr Gly Leu Lys Pro Thr Ala Ala Asp Lys Ser Asp 
    370                 375                 380                 


Ala Ile Lys Pro Thr Asn Thr Pro Val Phe Leu Lys Arg Thr Phe Thr 
385                 390                 395                 400 


Gln Thr Pro His Gly Val Arg Ala Leu Leu Asp Ile Thr Ser Ile Thr 
                405                 410                 415     


Arg Gln Phe Tyr Trp Leu Lys Ala Asn Arg Thr Ser Asp Pro Ser Ser 
            420                 425                 430         


Pro Pro Ala Phe Asp Arg Gln Ala Arg Ser Ala Gln Leu Glu Asn Ala 
        435                 440                 445             


Leu Ala Tyr Ala Ser Gln His Gly Pro Val Val Phe Asp Thr Val Arg 
    450                 455                 460                 


Gln Ile Ala Ile Lys Thr Ala Gln Gly Glu Gly Leu Val Leu Val Asn 
465                 470                 475                 480 


Thr Asn Tyr Asp Gln Ala Leu Ala Thr Tyr Asn Ala Trp Phe Ile Gly 
                485                 490                 495     


Gly Thr Val Pro Asp Pro Val Gly His Thr Glu Gly Thr His Lys Ile 
            500                 505                 510         


Val Phe Glu Met Glu His His His His His His 
        515                 520             


<210>  13
<211>  539
<212>  PRT
<213>  Artificial

<220>
<223>  Vesivirus RNA polymerase containing His tag

<400>  13

Met Lys Val Thr Thr Gln Lys Tyr Asp Val Thr Lys Pro Asp Ile Ser 
1               5                   10                  15      


Tyr Lys Gly Leu Ile Cys Lys Gln Leu Asp Glu Ile Arg Val Ile Pro 
            20                  25                  30          


Lys Gly Thr Arg Leu His Val Ser Pro Ala His Thr Asp Asp Tyr Asp 
        35                  40                  45              


Glu Cys Ser His Gln Pro Ala Ser Leu Gly Ser Gly Asp Pro Arg Cys 
    50                  55                  60                  


Pro Lys Ser Leu Thr Ala Ile Val Val Asp Ser Leu Lys Pro Tyr Cys 
65                  70                  75                  80  


Glu Lys Thr Asp Gly Pro Pro His Asp Ile Leu His Arg Val Gln Arg 
                85                  90                  95      


Met Leu Ile Asp His Leu Ser Gly Phe Val Pro Met Asn Ile Ser Ser 
            100                 105                 110         


Glu Pro Ser Met Leu Ala Ala Phe His Lys Leu Asn His Asp Thr Ser 
        115                 120                 125             


Cys Gly Pro Tyr Leu Gly Gly Arg Lys Lys Asp His Met Ile Gly Gly 
    130                 135                 140                 


Glu Pro Asp Lys Pro Leu Leu Asp Leu Leu Ser Ser Lys Trp Lys Leu 
145                 150                 155                 160 


Ala Thr Gln Gly Ile Gly Leu Pro His Glu Tyr Thr Ile Gly Leu Lys 
                165                 170                 175     


Asp Glu Leu Arg Pro Val Glu Lys Val Gln Glu Gly Lys Arg Arg Met 
            180                 185                 190         


Ile Trp Gly Cys Asp Val Gly Val Ala Thr Val Cys Ala Ala Ala Phe 
        195                 200                 205             


Lys Gly Val Ser Asp Ala Ile Thr Ala Asn His Gln Tyr Gly Pro Val 
    210                 215                 220                 


Gln Val Gly Ile Asn Met Asp Gly Pro Ser Val Glu Ala Leu Tyr Gln 
225                 230                 235                 240 


Arg Ile Arg Ser Ala Ala Lys Val Phe Ala Val Asp Tyr Ser Lys Trp 
                245                 250                 255     


Asp Ser Thr Gln Ser Pro Arg Val Ser Ala Ala Ser Ile Asp Ile Leu 
            260                 265                 270         


Arg Tyr Phe Ser Asp Arg Ser Pro Ile Val Asp Ser Ala Ala Asn Thr 
        275                 280                 285             


Leu Lys Ser Pro Pro Ile Ala Ile Phe Asn Gly Val Ala Val Lys Val 
    290                 295                 300                 


Thr Ser Gly Leu Pro Ser Gly Met Pro Leu Thr Ser Val Ile Asn Ser 
305                 310                 315                 320 


Leu Asn His Cys Leu Tyr Val Gly Cys Ala Ile Leu Gln Ser Leu Glu 
                325                 330                 335     


Ser Arg Asn Ile Pro Val Thr Trp Asn Leu Phe Ser Thr Phe Asp Met 
            340                 345                 350         


Met Thr Tyr Gly Asp Asp Gly Val Tyr Met Phe Pro Met Met Phe Ala 
        355                 360                 365             


Ser Val Ser Asp Gln Ile Phe Ala Asn Leu Thr Ala Tyr Gly Leu Lys 
    370                 375                 380                 


Pro Thr Arg Val Asp Lys Ser Val Gly Ala Ile Glu Pro Ile Asp Pro 
385                 390                 395                 400 


Glu Ser Val Val Phe Leu Lys Arg Thr Ile Thr Arg Thr Pro His Gly 
                405                 410                 415     


Ile Arg Gly Leu Leu Asp Arg Gly Ser Ile Ile Arg Gln Phe Tyr Tyr 
            420                 425                 430         


Ile Lys Gly Glu Asn Ser Asp Asp Trp Lys Thr Pro Pro Lys Thr Ile 
        435                 440                 445             


Asp Pro Thr Ser Arg Gly Gln Gln Leu Trp Asn Ala Cys Leu Tyr Ala 
    450                 455                 460                 


Ser Gln His Gly Pro Glu Phe Tyr Asn Lys Val Tyr Arg Leu Ala Glu 
465                 470                 475                 480 


Lys Ala Val Glu Tyr Glu Glu Leu His Phe Glu Pro Pro Ser Tyr His 
                485                 490                 495     


Ser Ala Leu Glu His Tyr Asn Asn Gln Phe Asn Gly Val Asp Thr Arg 
            500                 505                 510         


Ser Asp Gln Ile Asp Ala Ser Val Met Thr Asp Leu His Cys Asp Val 
        515                 520                 525             


Phe Glu Val Leu Glu His His His His His His 
    530                 535                 


<210>  14
<211>  523
<212>  PRT
<213>  Artificial

<220>
<223>  Lagovirus RNA Polymerase containing His tag

<400>  14

Met Thr Ser Asn Phe Phe Cys Gly Glu Pro Ile Asp Tyr Arg Gly Ile 
1               5                   10                  15      


Thr Ala His Arg Leu Val Gly Ala Glu Pro Arg Pro Pro Val Ser Gly 
            20                  25                  30          


Thr Arg Tyr Ala Lys Val Pro Gly Val Pro Asp Glu Tyr Lys Thr Gly 
        35                  40                  45              


Tyr Arg Pro Ala Asn Leu Gly Arg Ser Asp Pro Asp Ser Asp Lys Ser 
    50                  55                  60                  


Leu Met Asn Ile Ala Val Lys Asn Leu Gln Val Tyr Gln Gln Glu Pro 
65                  70                  75                  80  


Lys Leu Asp Lys Val Asp Glu Phe Ile Glu Arg Ala Ala Ala Asp Val 
                85                  90                  95      


Leu Gly Tyr Leu Arg Phe Leu Thr Lys Gly Glu Arg Gln Ala Asn Leu 
            100                 105                 110         


Asn Phe Lys Ala Ala Phe Asn Thr Leu Asp Leu Ser Thr Ser Cys Gly 
        115                 120                 125             


Pro Phe Val Pro Gly Lys Lys Ile Asp His Val Lys Asp Gly Val Met 
    130                 135                 140                 


Asp Gln Val Leu Ala Lys His Leu Tyr Lys Cys Trp Ser Val Ala Asn 
145                 150                 155                 160 


Ser Gly Lys Ala Leu His His Ile Tyr Ala Cys Gly Leu Lys Asp Glu 
                165                 170                 175     


Leu Arg Pro Leu Asp Lys Val Lys Glu Gly Lys Lys Arg Leu Leu Trp 
            180                 185                 190         


Gly Cys Asp Val Gly Val Ala Val Cys Ala Ala Ala Val Phe His Asn 
        195                 200                 205             


Ile Cys Tyr Lys Leu Lys Met Val Ala Arg Phe Gly Pro Ile Ala Val 
    210                 215                 220                 


Gly Val Asp Met Thr Ser Arg Asp Val Asp Val Ile Ile Asn Asn Leu 
225                 230                 235                 240 


Thr Ser Lys Ala Ser Asp Phe Leu Cys Leu Asp Tyr Ser Lys Trp Asp 
                245                 250                 255     


Ser Thr Met Ser Pro Cys Val Val Arg Leu Ala Ile Asp Ile Leu Ala 
            260                 265                 270         


Asp Cys Cys Glu Gln Thr Glu Leu Thr Lys Ser Val Val Leu Thr Leu 
        275                 280                 285             


Lys Ser His Pro Met Thr Ile Leu Asp Ala Met Ile Val Gln Thr Lys 
    290                 295                 300                 


Arg Gly Leu Pro Ser Gly Met Pro Phe Thr Ser Val Ile Asn Ser Ile 
305                 310                 315                 320 


Cys His Trp Leu Leu Trp Ser Ala Ala Val Tyr Lys Ser Cys Ala Glu 
                325                 330                 335     


Ile Gly Leu His Cys Ser Asn Leu Tyr Glu Asp Ala Pro Phe Tyr Thr 
            340                 345                 350         


Tyr Gly Asp Asp Gly Val Tyr Ala Met Thr Pro Met Met Val Ser Leu 
        355                 360                 365             


Leu Pro Ala Ile Ile Glu Asn Leu Arg Asp Tyr Gly Leu Ser Pro Thr 
    370                 375                 380                 


Ala Ala Asp Lys Thr Glu Phe Ile Asp Val Cys Pro Leu Asn Lys Ile 
385                 390                 395                 400 


Ser Phe Leu Lys Arg Thr Phe Glu Leu Thr Asp Ile Gly Trp Val Ser 
                405                 410                 415     


Lys Leu Asp Lys Ser Ser Ile Leu Arg Gln Leu Glu Trp Ser Lys Thr 
            420                 425                 430         


Thr Ser Arg His Met Val Ile Glu Glu Thr Tyr Asp Leu Ala Lys Glu 
        435                 440                 445             


Glu Arg Gly Val Gln Leu Glu Glu Leu Gln Val Ala Ala Ala Ala His 
    450                 455                 460                 


Gly Gln Glu Phe Phe Asn Phe Val Cys Arg Glu Leu Glu Arg Gln Gln 
465                 470                 475                 480 


Ala Tyr Thr Gln Phe Ser Val Tyr Ser Tyr Asp Ala Ala Arg Lys Ile 
                485                 490                 495     


Leu Ala Asp Arg Lys Arg Val Val Ser Val Val Pro Asp Asp Glu Phe 
            500                 505                 510         


Val Asn Val Met Glu His His His His His His 
        515                 520             


<210>  15
<211>  24
<212>  RNA
<213>  Artificial

<220>
<223>  ssRNA template

<400>  15
auaccuagaa ucugaccaac cccc                                            24


<210>  16
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  ssDNA template

<400>  16
atacctagaa tctgaccaac cccc                                            24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  ssDNA template having 5 rC at the 3'-end


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  deoxyribonucleotides

<220>
<221>  misc_feature
<222>  (20)..(24)
<223>  ribonucleotides

<400>  17
atacctagaa tctgaccaac cccc                                            24


