                         SEQUENCE LISTING

<110>  The Board of Regents of the University of Oklahoma
       Ballard, Jimmy D.
 
<120>  Bacterial Cells, Optimized Nucelotide Sequences and Methods for 
       Improved Expression of Recombinant Clostridium difficle Toxin B

<130>  50183-P001US

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  1
atgagcctgg tgaaccgtaa acagctggaa aaaatggcga atgtgcgttt tcgcgttcag       60

gaagatgaat acgttgcgat tctggatgcc ctggaagaat atcataacat gagcgaaaat      120

accgtggttg aaaaatacct gaaactgaaa gatatcaaca gtctgaccga tatctacatc      180

gatacgtaca aaaaatctgg ccgcaacaaa gccctgaaaa aattcaaaga atacctggtg      240

accgaagttc tggaactgaa aaacaataac ctgacgccgg tggagaaaaa cctgcacttt      300

gtttggatcg gcggtcagat caacgatacc gcaatcaatt acatcaacca gtggaaagat      360

gtgaactctg attacaacgt gaacgtgttt tatgatagta atgcgttcct gattaacacc      420

ctgaagaaaa ccatcgttga aagcgccacg aacgataccc tggaatcttt ccgtgaaaac      480

ctgaatgatc cgcgcttcga ttacaacaaa ttctaccgta aacgcatgga aatcatctac      540

gataaacaga aaaacttcat caactactac aaaacccagc gtgaagaaaa cccggatctg      600

atcatcgatg atatcgtgaa aatctacctg agtaacgaat acagcaaaga tatcgatgaa      660

ctgaacagct atatcgaaga atctctgaat aaagtgaccg aaaactctgg caatgatgtt      720

cgcaactttg aagaattcaa aggcggtgaa agttttaaac tgtacgaaca ggaactggtg      780

gaacgttgga atctggcggc cgcaagtgat atcctgcgca ttagcgcgct gaaagaagtg      840

ggcggtgttt atctggatgt tgatatgctg ccgggtattc agccggatct gttcgaaagc      900

atcgaaaaac cgagctctgt gaccgttgat ttttgggaaa tggtgaaact ggaagcaatc      960

atgaaataca aagaatacat cccgggctat acgagcgaac acttcgatat gctggatgaa     1020

gaagtgcaga gtagctttga atctgttctg gcgagtaaaa gcgataaaag tgaaattttc     1080

tctagtctgg gtgatatgga agcgagcccg ctggaagtta aaatcgcctt caactctaaa     1140

ggcatcatca accagggtct gatcagtgtg aaagattctt actgcagtaa cctgatcgtt     1200

aaacagatcg aaaaccgtta caaaatcctg aataactctc tgaacccggc cattagtgaa     1260

gataacgatt ttaataccac gaccaatgca ttcatcgata gcattatggc cgaagcaaac     1320

gcggataatg gccgttttat gatggaactg ggtaaatatc tgcgcgtggg ctttttcccg     1380

gatgttaaaa cgaccattaa cctgagcggt ccggaagcgt acgcggcagc atatcaggat     1440

ctgctgatgt tcaaagaagg cagtatgaat atccacctga ttgaagcgga tctgcgtaac     1500

ttcgaaatca gcaaaaccaa catctctcag agtacggaac aggaaatggc cagcctgtgg     1560

tctttcgatg atgcccgcgc aaaagcgcag ttcgaagaat acaagaaaaa ctacttcgaa     1620

ggcagcctgg gtgaagatga taatctggat ttttctcaga acaccgtggt tgataaagaa     1680

tacctgctgg aaaaaatcag ctctctggcg cgtagtagcg aacgcggtta catccattat     1740

attgtgcagc tgcagggcga taaaatcagc tacgaagcgg cctgcaatct gtttgcaaaa     1800

accccgtatg atagtgttct gttccagaaa aacattgaag atagcgaaat cgcgtattac     1860

tataatccgg gcgatggtga aatccaggaa atcgataaat acaaaatccc gagtatcatc     1920

agcgatcgcc cgaaaattaa actgaccttt atcggccacg gtaaagatga atttaacacg     1980

gatattttcg cgggcctgga tgtggatagc ctgtctaccg aaatcgaaac ggcaattgat     2040

ctggcgaaag aagatatctc tccgaaaagt atcgaaatca atctgctggg ttgtaacatg     2100

tttagttaca gcgtgaatgt tgaagaaacc tatccgggca aactgctgct gcgtgtgaaa     2160

gataaagttt ctgaactgat gccgtctatc agtcaggatt ctattatcgt gagtgccaat     2220

cagtatgaag ttcgcattaa cagcgaaggt cgtcgcgaac tgctggatca tagcggcgaa     2280

tggatcaaca aagaagaatc tatcatcaaa gatatctcta gtaaagaata catcagcttc     2340

aacccgaaag aaaacaaaat catcgtgaaa agtaaaaacc tgccggaact gagcaccctg     2400

ctgcaggaaa tccgtaataa cagcaatagc tctgatattg aactggaaga aaaagtgatg     2460

ctggcagaat gcgaaatcaa cgttatctct aacatcgata cccaggtggt tgaaggtcgc     2520

attgaagaag cgaaaagtct gacgagcgat tctatcaact acatcaaaaa cgaattcaaa     2580

ctgattgaaa gtatcagcga tgcgctgtat gatctgaaac agcagaacga actggaagaa     2640

agccacttta tttctttcga agatatcctg gaaaccgatg aaggtttctc tatccgtttc     2700

atcgataaag aaaccggcga aagtattttt gtggaaacgg aaaaagcaat cttcagcgaa     2760

tacgcgaacc atatcaccga agaaatctct aaaatcaaag gtaccatctt tgatacggtg     2820

aacggcaaac tggtgaaaaa agttaatctg gatgccaccc acgaagttaa cacgctgaat     2880

gcagcgtttt tcatccagag cctgattgaa tacaacagta gcaaagaatc tctgagtaat     2940

ctgagcgtgg caatgaaagt gcaggtttat gcgcagctgt tttctaccgg cctgaacacg     3000

attaccgatg ccgcaaaagt ggttgaactg gttagcaccg ccctggatga aacgattgat     3060

ctgctgccga ccctgagcga aggtctgccg gtgatcgcaa cgattatcga tggcgtttct     3120

ctgggtgcgg ccattaaaga actgagcgaa acctctgatc cgctgctgcg tcaggaaatt     3180

gaagccaaaa ttggcatcat ggcagtgaat ctgaccgcag cgacgaccgc aattatcacg     3240

tctagtctgg gcattgcgag tggttttagc atcctgctgg tgccgctggc aggtatcagt     3300

gcaggtattc cgagcctggt taataacgaa ctgatcctgc gcgataaagc gaccaaagtg     3360

gttgattact tctctcatat cagtctggcg gaaagcgaag gtgcctttac gtctctggat     3420

gataaaatta tgatgccgca ggatgatctg gtgatcagtg aaatcgattt caacaacaac     3480

agcattaccc tgggtaaatg tgaaatctgg cgtatggaag gcggtagcgg ccatacggtt     3540

accgatgata tcgatcactt tttcagcgcc ccgtctatta cctaccgcga accgcacctg     3600

agcatctatg atgtgctgga agttcagaaa gaagaactgg atctgtctaa agatctgatg     3660

gtgctgccga acgcgccgaa tcgtgttttc gcctgggaaa cgggttggac cccgggtctg     3720

cgtagcctgg aaaacgatgg taccaaactg ctggatcgta ttcgcgataa ttacgaaggc     3780

gaattttact ggcgttattt tgcgttcatt gccgatgcac tgatcacgac cctgaaaccg     3840

cgttatgaag ataccaacat tcgcatcaat ctggatagta acacgcgtag cttcatcgtg     3900

ccggttatta cgaccgaata tattcgcgaa aaactgagct actcttttta tggcagcggc     3960

ggtacctacg ccctgagtct gagccagtac aacatgaaca tcaacatcga actgaacgaa     4020

aacgatacct gggtgattga tgttgataac gtggttcgcg atgtgacgat cgaaagcgat     4080

aaaatcaaaa aaggtgatct gatcgaaaac atcctgagta aactgagcat cgaagataac     4140

aaaatcatcc tggataacca tgaaatcaac tttagcggta ccctgaacgg cggtaatggc     4200

tttgtttctc tgacgttcag tatcctggaa ggtattaatg cggtgatcga agttgatctg     4260

ctgtctaaaa gttataaagt gctgattagc ggcgaactga aaaccctgat ggccaatagc     4320

aactctgttc agcagaaaat tgattacatc ggtctgaata gtgaactgca gaaaaacatc     4380

ccgtatagct ttatggatga taaaggcaaa gaaaacggtt tcatcaactg cagcaccaaa     4440

gaaggcctgt ttgtgtctga actgagtgat gtggttctga tctctaaagt ttacatggat     4500

aacagtaaac cgctgtttgg ctattgtagc aatgatctga aagatgtgaa agttatcacc     4560

aaagatgatg tgatcatcct gacgggttac tacctgaaag atgatatcaa aatcagtctg     4620

agcttcacca ttcaggatga aaatacgatc aaactgaacg gtgtgtatct ggatgaaaac     4680

ggcgttgcgg aaatcctgaa attcatgaac aaaaaaggca gcacgaacac ctctgatagt     4740

ctgatgagct tcctggaatc tatgaacatc aaatctatct tcatcaatag cctgcagtct     4800

aacaccaaac tgatcctgga tacgaatttc atcatcagtg gcacgaccag cattggccag     4860

ttcgaattca tctgcgataa agataacaac atccagccgt acttcatcaa attcaacacg     4920

ctggaaacca aatacacgct gtatgtgggt aaccgtcaga atatgattgt tgaaccgaac     4980

tatgatctgg atgatagtgg tgatattagc tctaccgtga tcaattttag ccagaaatac     5040

ctgtatggca tcgattcttg tgtgaacaaa gttatcatca gtccgaacat ctacaccgat     5100

gaaatcaaca tcacgccgat ctacgaagcg aacaatacct atccggaagt gattgttctg     5160

gatacgaact acatctctga aaaaatcaac atcaacatca acgatctgag tattcgttat     5220

gtgtggtcta acgatggcag tgattttatc ctgatgagca ccgatgaaga aaacaaagtg     5280

tctcaggtta aaatccgctt caccaacgtt ttcaagggta acacgatcag cgataaaatc     5340

tctttcaact tcagtgataa acaggatgtg agcatcaaca aagttatctc tacgttcacc     5400

ccgagttact atgtggaagg tctgctgaac tacgatctgg gcctgattag cctgtacaac     5460

gaaaaattct acatcaacaa cttcggcatg atggtgtctg gtctggttta catcaacgat     5520

agcctgtact acttcaaacc gccgattaaa aatctgatca ccggcttcac gaccatcggt     5580

gatgataaat actacttcaa cccggataat ggcggtgccg caagcgtggg tgaaaccatc     5640

atcgatggca aaaactacta cttcagccag aacggcgtgc tgcagaccgg tgtgtttagc     5700

acggaagatg gctttaaata tttcgcgccg gccgataccc tggatgaaaa tctggaaggt     5760

gaagcgatcg atttcaccgg caaactgacg attgatgaaa acgtgtacta cttcggtgat     5820

aactatcgtg cggccattga atggcagacc ctggatgatg aagtttacta cttctctacg     5880

gataccggcc gcgccttcaa aggtctgaac cagatcggcg atgataaatt ctacttcaac     5940

agcgatggta tcatgcagaa aggcttcgtg aacatcaacg ataaaacctt ctacttcgat     6000

gatagcggtg ttatgaaatc tggctatacg gaaatcgatg gcaaatactt ttatttcgcg     6060

gaaaacggtg aaatgcagat tggcgtgttt aataccgccg atggttttaa atacttcgca     6120

catcacgatg aagatctggg caacgaagaa ggtgaagccc tgagctactc tggcattctg     6180

aacttcaaca acaaaatcta ctacttcgat gatagtttca ccgcagtggt tggttggaaa     6240

gatctggaag atggcagcaa atactatttt gatgaagata cggcagaagc gtatatcggt     6300

atctctatca tcaacgatgg caaatactac ttcaacgata gtggtatcat gcagatcggc     6360

ttcgtgacca tcaacaacga agtgttttat ttcagtgata gcggtatcgt ggaaagcggc     6420

atgcagaaca tcgatgacaa ctacttctac atcgatgaaa acggtctggt gcagattggc     6480

gttttcgata cctctgatgg ttacaaatac ttcgccccgg caaatacggt gaacgataat     6540

atctacggcc aggcagttga atatagcggt ctggtgcgtg ttggcgaaga tgtgtactat     6600

tttggtgaaa cgtacaccat tgaaaccggc tggatctatg atatggaaaa cgaaagcgat     6660

aaatactact tcgatccgga aacgaaaaaa gcgtacaaag gcatcaacgt tatcgatgat     6720

atcaaatact acttcgatga aaacggtatt atgcgcaccg gcctgatcac gttcgaagat     6780

aaccattact acttcaacga agatggtatc atgcagtacg gctacctgaa catcgaagac     6840

aaaacgttct acttctctga agacggtatc atgcagattg gcgtgttcaa taccccggat     6900

ggttttaaat atttcgccca ccagaatacg ctggatgaaa acttcgaagg tgaaagcatc     6960

aactacaccg gctggctgga tctggatgaa aaacgctact atttcaccga tgaatatatc     7020

gcagcgacgg gtagtgtgat tatcgatggc gaagaatact attttgatcc ggataccgca     7080

cagctggtta ttagcgaata a                                               7101


<210>  2
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  2
atgagtttag ttaatagaaa acagttagaa aaaatggcaa atgtaagatt tcgtgttcag       60

gaagatgaat atgtagcaat attggatgct ttagaagaat atcataatat gtcagagaat      120

actgtagtcg aaaaatattt aaaattaaaa gatataaata gtttaacaga tatttatata      180

gatacatata aaaagtctgg tagaaataaa gccttaaaaa aatttaagga atacctagtt      240

acagaagtat tagagctaaa gaataataat ttaactccag ttgagaaaaa tttacatttt      300

gtttggattg gaggtcaaat aaatgacact gctattaatt atataaatca atggaaagat      360

gtaaatagtg attataatgt taatgttttt tatgatagta atgcattttt gataaacaca      420

ttgaaaaaaa ctatagtaga atcagcaaca aatgatacac ttgaatcatt tagagaaaac      480

ttaaatgacc ctagatttga ctataataaa ttttacagaa aacgtatgga gataatctat      540

gataagcaga aaaatttcat aaattactat aaaactcaaa gagaagaaaa tcctgacctt      600

ataattgatg atattgtaaa gatatatctt tcaaacgagt attcaaagga tatagatgaa      660

cttaattcct atattgaaga gtcattaaat aaagttacag aaaatagtgg gaatgatgtt      720

agaaactttg aagaatttaa aggtggagag tcattcaaat tatatgaaca agagttggta      780

gaaagatgga atttggcagc tgcttctgac atattaagaa tatctgcctt aaaagaagtt      840

ggtggtgtat atttagatgt tgatatgtta ccaggaatac aaccagacct atttgagtct      900

atagagaaac ctagttcagt aacagtggat ttttgggaaa tggtaaaatt agaagctata      960

atgaaataca aagaatacat accaggatat acttcagagc attttgatat gttggatgaa     1020

gaagttcaaa gtagttttga atctgttcta gcttctaagt cagataagtc agaaatattc     1080

tcatcacttg gcgatatgga ggcatcacca ctagaagtta aaattgcatt taatagtaaa     1140

ggtattataa atcaaggact aatttctgtg aaagactcat attgtagcaa tttaatagta     1200

aaacaaatcg agaacagata taagatattg aataatagtt taaatccagc tattagcgag     1260

gataacgatt tcaatactac aacgaatgct tttattgata gtataatggc tgaagctaat     1320

gcagataatg gtagatttat gatggaacta ggaaagtatt taagagttgg tttcttccca     1380

gatgttaaaa ctactattaa cttaagtggc cctgaagcat atgcggcagc ttatcaagat     1440

ttattaatgt ttaaagaagg tagtatgaat atccatttga tagaagctga cttaagaaac     1500

tttgaaatct ctaaaactaa tatttctcaa tcaactgaac aagaaatggc tagcttatgg     1560

tcatttgacg atgcaagagc taaagctcaa tttgaagaat acaaaaaaaa ttactttgaa     1620

ggttctcttg gagaagatga caatcttgac ttttctcaaa atacagtagt tgacaaggag     1680

tatcttttag aaaaaatatc ttcattagca agaagttcag agagaggata tatacactat     1740

attgttcagt tacaaggaga taaaattagt tatgaagcag catgtaactt atttgcaaag     1800

actccttatg atagtgtact gtttcagaaa aatatagaag attcagaaat tgcatattat     1860

tataatcctg gagatggtga aatacaagaa atagacaagt ataaaattcc aagtataatt     1920

tctgatagac ctaagattaa attaacattt attggtcatg gtaaagatga atttaatact     1980

gatatatttg caggtcttga tgtagattca ttatccacag aaatagaaac agcaatagat     2040

ttagctaaag aggatatttc tcctaagtca atagaaataa acttactggg atgtaacatg     2100

tttagctatt ctgtaaatgt agaggagact tatcctggga aattattact tagagttaaa     2160

gataaagtat cagaattaat gccatctata agtcaagact ctattatagt aagtgcaaat     2220

caatatgaag ttagaataaa tagtgaagga agaagagaat tattggatca ttctggtgaa     2280

tggataaata aagaagaaag tattataaag gatatttcat caaaagaata tatatcattt     2340

aatcctaaag aaaataaaat tatagtaaaa tctaaaaatt tacctgaatt atctacatta     2400

ttacaagaaa ttagaaacaa ttctaattca agtgatattg aactagaaga aaaagtaatg     2460

ttagcagaat gtgagataaa tgttatttca aatatagata cacaagtggt agaaggaagg     2520

attgaagaag ctaaaagctt aacttctgac tctattaatt atataaagaa tgaatttaaa     2580

ctaatagaat ctatttctga tgcactatac gatttaaaac aacagaatga attagaagag     2640

tctcatttta tatcttttga ggatatattg gagactgatg aaggctttag tataagattt     2700

attgataaag aaactggaga atctatattt gtagaaactg aaaaggcaat attctctgaa     2760

tatgctaatc atataactga agagatttct aagataaaag gtactatatt tgatactgta     2820

aatggtaagt tagtaaaaaa agtaaattta gatgctacac atgaagtgaa tactttaaat     2880

gctgcatttt ttatacaatc attaatagaa tataatagtt ctaaagaatc tcttagtaat     2940

ttaagtgtag caatgaaagt tcaagtttat gctcaattat ttagtactgg tttaaatact     3000

attacagatg cagccaaagt tgttgaatta gtatcaactg cattagatga aactatagat     3060

ttacttccta cattatctga aggattacct gtaattgcaa ctatcataga tggtgtaagt     3120

ttaggtgcag caatcaaaga gctaagtgaa acaagtgacc cattattaag acaagaaata     3180

gaagctaaga taggtataat ggcagtaaat ttaacagcag ctacaactgc aatcattact     3240

tcatctttag gaatagctag tggatttagt atacttttag ttcctttagc aggaatttca     3300

gcaggtatac caagtttagt aaacaatgag cttatcctaa gagataaagc aacaaaagtt     3360

gtagattatt ttagtcatat atcattagct gagtctgaag gagcatttac ttcattagat     3420

gataaaataa tgatgccaca agatgattta gtaatatctg agatagactt taataacaat     3480

tcaataactt taggtaaatg tgaaatctgg agaatggaag gtggctcagg tcatactgta     3540

actgatgata tagatcactt cttttcagca ccatcaataa catatagaga gccacactta     3600

tctatatatg acgtattgga agtacaaaaa gaagaacttg atttgtcaaa agatttaatg     3660

gtattaccta atgctccaaa tagagtattt gcttgggaaa caggatggac accaggttta     3720

agaagcttag aaaatgatgg cacaaaactg ttagaccgta taagagataa ctatgaaggt     3780

gagttttatt ggagatattt tgcttttata gctgatgctt taataacaac attaaaacca     3840

agatatgaag atactaatat aagaataaat ttagatagta atactagaag ctttatagtc     3900

ccagtgataa ctacagaata tataagagag aaattatcat attcttttta tggttctgga     3960

ggaacttatg cattatctct ttctcaatac aatatgaata taaacataga attaaatgaa     4020

aatgatactt gggttataga tgtcgacaat gtcgtaagag atgtcactat agaatctgat     4080

aaaattaaaa aaggagattt aatagagaat attttatcta aattaagtat tgaagacaat     4140

aaaattattt tagataatca tgaaattaat ttctctggaa cattaaatgg aggtaatgga     4200

tttgtatctt taacattctc aatcttagaa ggaataaatg cagttataga agttgattta     4260

ttatctaaat catataaagt tcttatttct ggtgaactaa aaacattgat ggcaaattca     4320

aattctgttc aacagaaaat agattatata ggattgaaca gcgaattaca aaaaaatata     4380

ccttatagtt ttatggatga taaaggaaaa gaaaatggat ttattaattg ttctacaaaa     4440

gaaggtttat ttgtatctga attatctgat gtagttctta taagtaaagt ttatatggac     4500

aatagtaaac ctctatttgg atattgtagt aatgatttga aagatgttaa agtcataact     4560

aaagatgacg ttattatatt aacaggatat tatttaaaag atgatataaa aatctctctt     4620

tcttttacta tacaagatga aaatactata aaattaaatg gagtatattt agatgaaaat     4680

ggagtagctg aaatattgaa atttatgaat aaaaaaggta gtacaaatac ttcagattct     4740

ttaatgagct ttttagaaag tatgaatata aaaagtattt tcataaattc cttacaatct     4800

aatactaagc ttatattaga tactaatttt ataataagtg gtactacttc tattggtcaa     4860

tttgagttta tttgtgataa agataataat atacaaccat atttcattaa gtttaataca     4920

ctagaaacta aatatactct atatgtaggt aatagacaaa atatgatagt agaaccaaat     4980

tatgatttag atgattctgg agatatatct tcaactgtca ttaatttttc tcagaaatac     5040

ctttatggaa tagacagttg tgttaataaa gttataattt cgccaaatat atatacagat     5100

gaaataaaca taacacctat atatgaagca aataatactt atccagaagt gattgtatta     5160

gatacaaatt atataagtga aaaaatcaat attaatatca atgatttatc tatacgatat     5220

gtatggagta atgatggaag tgattttatt cttatgtcaa ctgatgaaga gaacaaggta     5280

tcacaagtta aaataagatt tactaatgtt tttaaaggta atactatatc agataagata     5340

tcttttaatt ttagtgataa gcaagatgta tctataaata aagttatttc aacatttaca     5400

ccttcatatt atgtggaagg attacttaat tatgatttag gtctgatttc tttatacaat     5460

gagaaatttt atattaataa ctttggaatg atggtgtctg gattagtata tattaatgat     5520

tcattatatt atttcaagcc accaataaag aacttgataa ctggatttac aactataggt     5580

gatgataaat actactttaa tccagataat ggtggagctg cttcagtcgg agaaacaata     5640

attgatggca aaaactacta cttcagccaa aatggagtgt tacaaacagg tgtatttagt     5700

acagaagatg gatttaaata ttttgctcca gcagatacac ttgatgaaaa tctagagggg     5760

gaagcaattg attttactgg caaactaact attgatgaaa atgtttatta ttttggagat     5820

aattatagag cagctataga atggcaaaca ttagatgatg aagtgtacta ttttagtaca     5880

gatacaggta gagcttttaa agggctaaat caaataggtg atgataaatt ctatttcaac     5940

tctgatggta ttatgcaaaa aggatttgtt aatataaatg ataagacatt ctattttgat     6000

gattctggtg tgatgaagtc aggatatact gaaatagatg gaaaatattt ttactttgct     6060

gagaatggag aaatgcaaat aggagtattt aatacagcag atggatttaa atattttgct     6120

catcatgatg aagatttagg aaatgaagaa ggtgaagcac tttcatattc tggtatactt     6180

aattttaaca ataagattta ttattttgat gattcattta cagcagtagt tggatggaag     6240

gatttagaag atggttcaaa atattacttt gatgaagata cagcagaagc atatataggt     6300

atctcaataa ttaatgatgg taaatattat tttaatgatt ctggaatcat gcaaattgga     6360

tttgtcacaa taaataatga agtattttat ttctctgatt ctggaatagt agaatctgga     6420

atgcaaaata tagatgataa ttatttctat atagacgaaa atggtctagt tcaaattggt     6480

gtatttgaca cttcagatgg atataaatac tttgcaccag ctaatactgt aaatgataat     6540

atctatggac aagcagttga atatagtggt ttagttagag ttggtgaaga tgtatattat     6600

tttggagaaa catatacaat tgagactggt tggatatatg atatggaaaa tgaaagtgat     6660

aaatattatt tcgatccaga aactaaaaaa gcatataaag gtattaatgt aattgatgat     6720

ataaaatact attttgatga gaatggaata atgagaacag gtcttataac atttgaagat     6780

aatcattact attttaatga agatggtatt atgcaatatg gttatctaaa tatagaagat     6840

aagacgttct actttagtga agatggtatt atgcagattg gagtatttaa tacaccagat     6900

ggatttaaat attttgcaca tcaaaatact ttagatgaga attttgaggg agaatcaata     6960

aactatactg gttggttaga tttagatgaa aagagatatt attttacaga tgaatatatt     7020

gcagcaactg gttcagttat tattgatggt gaggagtatt attttgatcc tgatacagct     7080

caattagtga ttagtgaata g                                               7101


<210>  3
<211>  2166
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(2166)

<400>  3
atgctgtatg tgggtaaccg tcagaatatg attgttgaac cgaactatga tctggatgat       60

agtggtgata ttagctctac cgtgatcaat tttagccaga aatacctgta tggcatcgat      120

tcttgtgtga acaaagttat catcagtccg aacatctaca ccgatgaaat caacatcacg      180

ccgatctacg aagcgaacaa tacctatccg gaagtgattg ttctggatac gaactacatc      240

tctgaaaaaa tcaacatcaa catcaacgat ctgagtattc gttatgtgtg gtctaacgat      300

ggcagtgatt ttatcctgat gagcaccgat gaagaaaaca aagtgtctca ggttaaaatc      360

cgcttcacca acgttttcaa gggtaacacg atcagcgata aaatctcttt caacttcagt      420

gataaacagg atgtgagcat caacaaagtt atctctacgt tcaccccgag ttactatgtg      480

gaaggtctgc tgaactacga tctgggcctg attagcctgt acaacgaaaa attctacatc      540

aacaacttcg gcatgatggt gtctggtctg gtttacatca acgatagcct gtactacttc      600

aaaccgccga ttaaaaatct gatcaccggc ttcacgacca tcggtgatga taaatactac      660

ttcaacccgg ataatggcgg tgccgcaagc gtgggtgaaa ccatcatcga tggcaaaaac      720

tactacttca gccagaacgg cgtgctgcag accggtgtgt ttagcacgga agatggcttt      780

aaatatttcg cgccggccga taccctggat gaaaatctgg aaggtgaagc gatcgatttc      840

accggcaaac tgacgattga tgaaaacgtg tactacttcg gtgataacta tcgtgcggcc      900

attgaatggc agaccctgga tgatgaagtt tactacttct ctacggatac cggccgcgcc      960

ttcaaaggtc tgaaccagat cggcgatgat aaattctact tcaacagcga tggtatcatg     1020

cagaaaggct tcgtgaacat caacgataaa accttctact tcgatgatag cggtgttatg     1080

aaatctggct atacggaaat cgatggcaaa tacttttatt tcgcggaaaa cggtgaaatg     1140

cagattggcg tgtttaatac cgccgatggt tttaaatact tcgcacatca cgatgaagat     1200

ctgggcaacg aagaaggtga agccctgagc tactctggca ttctgaactt caacaacaaa     1260

atctactact tcgatgatag tttcaccgca gtggttggtt ggaaagatct ggaagatggc     1320

agcaaatact attttgatga agatacggca gaagcgtata tcggtatctc tatcatcaac     1380

gatggcaaat actacttcaa cgatagtggt atcatgcaga tcggcttcgt gaccatcaac     1440

aacgaagtgt tttatttcag tgatagcggt atcgtggaaa gcggcatgca gaacatcgat     1500

gacaactact tctacatcga tgaaaacggt ctggtgcaga ttggcgtttt cgatacctct     1560

gatggttaca aatacttcgc cccggcaaat acggtgaacg ataatatcta cggccaggca     1620

gttgaatata gcggtctggt gcgtgttggc gaagatgtgt actattttgg tgaaacgtac     1680

accattgaaa ccggctggat ctatgatatg gaaaacgaaa gcgataaata ctacttcgat     1740

ccggaaacga aaaaagcgta caaaggcatc aacgttatcg atgatatcaa atactacttc     1800

gatgaaaacg gtattatgcg caccggcctg atcacgttcg aagataacca ttactacttc     1860

aacgaagatg gtatcatgca gtacggctac ctgaacatcg aagacaaaac gttctacttc     1920

tctgaagacg gtatcatgca gattggcgtg ttcaataccc cggatggttt taaatatttc     1980

gcccaccaga atacgctgga tgaaaacttc gaaggtgaaa gcatcaacta caccggctgg     2040

ctggatctgg atgaaaaacg ctactatttc accgatgaat atatcgcagc gacgggtagt     2100

gtgattatcg atggcgaaga atactatttt gatccggata ccgcacagct ggttattagc     2160

gaataa                                                                2166


<210>  4
<211>  2166
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(2166)

<400>  4
actctatatg taggtaatag acaaaatatg atagtagaac caaattatga tttagatgat       60

tctggagata tatcttcaac tgtcattaat ttttctcaga aataccttta tggaatagac      120

agttgtgtta ataaagttat aatttcgcca aatatatata cagatgaaat aaacataaca      180

cctatatatg aagcaaataa tacttatcca gaagtgattg tattagatac aaattatata      240

agtgaaaaaa tcaatattaa tatcaatgat ttatctatac gatatgtatg gagtaatgat      300

ggaagtgatt ttattcttat gtcaactgat gaagagaaca aggtatcaca agttaaaata      360

agatttacta atgtttttaa aggtaatact atatcagata agatatcttt taattttagt      420

gataagcaag atgtatctat aaataaagtt atttcaacat ttacaccttc atattatgtg      480

gaaggattac ttaattatga tttaggtctg atttctttat acaatgagaa attttatatt      540

aataactttg gaatgatggt gtctggatta gtatatatta atgattcatt atattatttc      600

aagccaccaa taaagaactt gataactgga tttacaacta taggtgatga taaatactac      660

tttaatccag ataatggtgg agctgcttca gtcggagaaa caataattga tggcaaaaac      720

tactacttca gccaaaatgg agtgttacaa acaggtgtat ttagtacaga agatggattt      780

aaatattttg ctccagcaga tacacttgat gaaaatctag agggggaagc aattgatttt      840

actggcaaac taactattga tgaaaatgtt tattattttg gagataatta tagagcagct      900

atagaatggc aaacattaga tgatgaagtg tactatttta gtacagatac aggtagagct      960

tttaaagggc taaatcaaat aggtgatgat aaattctatt tcaactctga tggtattatg     1020

caaaaaggat ttgttaatat aaatgataag acattctatt ttgatgattc tggtgtgatg     1080

aagtcaggat atactgaaat agatggaaaa tatttttact ttgctgagaa tggagaaatg     1140

caaataggag tatttaatac agcagatgga tttaaatatt ttgctcatca tgatgaagat     1200

ttaggaaatg aagaaggtga agcactttca tattctggta tacttaattt taacaataag     1260

atttattatt ttgatgattc atttacagca gtagttggat ggaaggattt agaagatggt     1320

tcaaaatatt actttgatga agatacagca gaagcatata taggtatctc aataattaat     1380

gatggtaaat attattttaa tgattctgga atcatgcaaa ttggatttgt cacaataaat     1440

aatgaagtat tttatttctc tgattctgga atagtagaat ctggaatgca aaatatagat     1500

gataattatt tctatataga cgaaaatggt ctagttcaaa ttggtgtatt tgacacttca     1560

gatggatata aatactttgc accagctaat actgtaaatg ataatatcta tggacaagca     1620

gttgaatata gtggtttagt tagagttggt gaagatgtat attattttgg agaaacatat     1680

acaattgaga ctggttggat atatgatatg gaaaatgaaa gtgataaata ttatttcgat     1740

ccagaaacta aaaaagcata taaaggtatt aatgtaattg atgatataaa atactatttt     1800

gatgagaatg gaataatgag aacaggtctt ataacatttg aagataatca ttactatttt     1860

aatgaagatg gtattatgca atatggttat ctaaatatag aagataagac gttctacttt     1920

agtgaagatg gtattatgca gattggagta tttaatacac cagatggatt taaatatttt     1980

gcacatcaaa atactttaga tgagaatttt gagggagaat caataaacta tactggttgg     2040

ttagatttag atgaaaagag atattatttt acagatgaat atattgcagc aactggttca     2100

gttattattg atggtgagga gtattatttt gatcctgata cagctcaatt agtgattagt     2160

gaatag                                                                2166


<210>  5
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  5
atgagcctgg tgaaccgtaa acagctggaa aaaatggcga acgtgcgttt tcgtacccag       60

gaagatgaat atgtggcgat tctggatgcg ctggaagaat atcataacat gagcgaaaac      120

accgtggtgg aaaaatacct gaaactgaaa gatatcaaca gcctgaccga tatctacatc      180

gatacctaca agaaaagcgg ccgtaacaaa gcgctgaaaa aatttaaaga atatctggtg      240

accgaagtgc tggaactgaa aaacaacaac ctgaccccgg tggaaaagaa cctgcatttt      300

gtgtggattg gcggccagat taacgatacc gcgattaact atattaacca gtggaaagat      360

gtgaacagcg attataacgt gaacgtgttt tatgatagca acgcgtttct gattaacacc      420

ctgaagaaaa ccgtggtgga aagcgcgatt aacgataccc tggaaagctt tcgtgaaaac      480

ctgaacgatc cgcgtttcga ttacaacaaa tttttccgta aacgtatgga aatcatctac      540

gataaacaga aaaactttat taactattat aaagcgcagc gtgaagaaaa cccggaactg      600

atcatcgatg atatcgtgaa aacctacctg agcaacgaat acagcaaaga aatcgatgaa      660

ctgaacacct acatcgaaga aagcctgaac aaaatcaccc agaacagcgg caacgatgtg      720

cgtaactttg aagaatttaa aaacggcgaa agctttaacc tgtatgaaca ggaactggtg      780

gaacgttgga acctggcggc ggcgagcgat attctgcgta ttagcgcgct gaaagaaatt      840

ggcggcatgt atctggatgt ggatatgctg ccgggcattc agccggatct gtttgaaagc      900

attgaaaaac cgagcagcgt gaccgtggat ttttgggaaa tgaccaaact ggaagcgatc      960

atgaaataca aagaatacat cccggaatac accagcgaac atttcgatat gctggatgaa     1020

gaagtgcaga gcagctttga aagcgtgctg gcgagcaaaa gcgataaaag cgaaattttt     1080

agcagcctgg gcgatatgga agcgagcccg ctggaagtga aaattgcgtt taacagcaaa     1140

ggcattatta accagggcct gattagcgtg aaagatagct attgcagcaa cctgattgtg     1200

aaacagattg aaaaccgtta taaaattctg aacaacagcc tgaacccggc gattagcgaa     1260

gataacgatt ttaacaccac caccaacacc tttattgata gcattatggc ggaagcgaac     1320

gcggataacg gccgttttat gatggaactg ggcaaatatc tgcgtgtggg tttctttccg     1380

gatgtgaaaa ccaccattaa cctgagcggc ccggaagcgt atgcggcggc gtatcaggat     1440

ctgctgatgt ttaaagaagg cagcatgaac attcatctga ttgaagcgga tctgcgtaac     1500

tttgaaatta gcaaaaccaa cattagccag agcaccgaac aggaaatggc gagcctgtgg     1560

agctttgatg atgcgcgtgc gaaagcgcag tttgaagaat ataaacgtaa ctattttgaa     1620

ggcagcctgg gcgaagatga taacctggat tttagccaga acattgtggt ggataaagaa     1680

tatctgctgg aaaaaattag cagcctggcg cgtagcagcg aacgtggcta tattcattat     1740

attgtgcagc tgcagggcga taaaattagc tatgaagcgg cgtgcaacct gtttgcgaaa     1800

accccgtatg atagcgtgct gtttcagaaa aacattgaag atagcgaaat tgcgtattat     1860

tataacccgg gcgatggcga aatccaggaa atcgataaat acaaaatccc gagcatcatc     1920

agcgatcgtc cgaaaatcaa actgaccttt attggccatg gcaaagatga atttaacacc     1980

gatatttttg cgggctttga tgtggatagc ctgagcaccg aaatcgaagc ggcgattgat     2040

ctggcgaaag aagatattag cccgaaaagc attgaaatta acctgctggg ctgcaacatg     2100

tttagctata gcattaacgt ggaagaaacc tatccgggca aactgctgct gaaagtgaaa     2160

gataaaatta gcgaactgat gccgagcatt agccaggata gcattattgt gagcgcgaac     2220

cagtatgaag tgcgtattaa cagcgaaggc cgtcgtgaac tgctggatca tagcggcgaa     2280

tggatcaaca aagaagaaag catcatcaaa gatatcagca gcaaagaata catcagcttc     2340

aacccgaaag aaaacaaaat caccgtgaaa agcaaaaacc tgccggaact gagcaccctg     2400

ctgcaggaaa ttcgtaacaa cagcaacagc agcgatattg aactggaaga aaaagtgatg     2460

ctgaccgaat gcgaaattaa cgtgattagc aacattgata cccagattgt ggaagaacgt     2520

attgaagaag cgaaaaacct gaccagcgat agcattaact atattaaaga tgaatttaaa     2580

ctgattgaaa gcattagcga tgcgctgtgc gatctgaaac agcagaacga actggaagac     2640

agccatttta ttagctttga agatattagc gaaaccgatg aaggctttag cattcgtttt     2700

attaacaaag aaaccggcga aagcattttt gtggaaaccg aaaaaaccat ttttagcgaa     2760

tacgcgaacc atatcaccga agaaatcagc aaaatcaaag gcaccatttt tgataccgtg     2820

aacggcaaac tggtgaaaaa agtgaacctg gataccaccc atgaagtgaa caccctgaac     2880

gcggcatttt tcattcagag cctgattgaa tataacagca gcaaagaaag cctgagcaac     2940

ctgagcgtgg cgatgaaagt gcaggtgtat gcgcagctgt ttagcaccgg cctgaacacc     3000

attaccgacg cggcgaaagt ggtggaactg gttagcaccg cgctggatga aaccattgat     3060

ctgctgccga ccctgagcga aggcctgccg attattgcga ccattattga tggcgtgagc     3120

ctgggcgcgg cgattaaaga actgagcgaa accagcgatc cgctgctgcg tcaggaaatt     3180

gaagcgaaaa ttggcattat ggcggtgaac ctgaccaccg cgaccaccgc gattattacc     3240

agcagcctgg gcattgcgag cggctttagc attctgctgg tgccgctggc gggcattagc     3300

gcgggcattc cgagcctggt gaacaacgaa ctggtgctgc gtgataaagc gaccaaagtg     3360

gtggattatt ttaaacatgt gagcctggtg gaaaccgaag gcgtgtttac cctgctggat     3420

gataaaatta tgatgccgca ggatgatctg gtgattagcg aaattgattt taacaacaac     3480

agcattgtgc tgggcaaatg cgaaatttgg cgtatggaag gcggcagcgg ccataccgtg     3540

accgatgata ttgatcattt ctttagcgcg ccgagcatta cctatcgtga accgcatctg     3600

agcatttatg atgtgctgga agtgcagaaa gaagaactgg atctgagcaa agatctgatg     3660

gtgctgccga acgcaccgaa ccgtgtgttt gcatgggaaa ccggttggac cccgggtctg     3720

cgtagcctgg aaaacgatgg caccaaactg ctggatcgta ttcgtgataa ctatgaaggc     3780

gaattttatt ggcgttattt tgcgtttatt gcggatgcgc tgattaccac cctgaaaccg     3840

cgttatgaag ataccaacat tcgtattaac ctggatagca acacccgtag ctttattgtg     3900

ccgattatta ccaccgaata tattcgtgaa aaactgagct atagctttta tggcagcggc     3960

ggcacctatg cgctgagcct gagccagtat aacatgggca ttaacattga actgagcgaa     4020

agcgatgtgt ggattattga tgtggataac gtggtgcgtg atgtgaccat tgaaagcgat     4080

aaaattaaaa aaggcgatct gattgaaggc attctgagca ccctgagcat tgaagaaaac     4140

aaaattattc tgaacagcca tgaaattaac tttagcggcg aagtgaacgg cagcaacggc     4200

tttgtgagcc tgacctttag cattctggaa ggcattaacg cgattattga agtggatctg     4260

ctgagcaaaa gctataaact gctgattagc ggcgaactga aaattctgat gctgaacagc     4320

aaccatattc agcagaaaat tgattatatt ggctttaaca gcgaactgca gaaaaacatt     4380

ccgtatagct ttgtggatag cgaaggcaaa gaaaacggct ttattaacgg cagcaccaaa     4440

gaaggcctgt ttgtgagcga actgccggat gtggtgctga ttagcaaagt gtatatggat     4500

gatagcaaac cgagctttgg ctattatagc aacaacctga aagatgtgaa agtgattacc     4560

aaagataacg tgaacattct gaccggctat tatctgaaag atgatattaa aattagcctg     4620

agcctgaccc tgcaggatga aaaaaccatt aaactgaaca gcgtgcatct ggatgaaagc     4680

ggcgtggcgg aaattctgaa atttatgaac cgtaaaggca acaccaacac cagcgatagc     4740

ctgatgagct ttctggaaag catgaacatc aaaagcatct tcgtgaactt tctgcagagc     4800

aacatcaaat tcatcctgga tgcgaacttc atcatcagcg gcaccaccag cattggccag     4860

ttcgaattta tttgcgatga aaacgataac atccagccgt acttcatcaa attcaacacc     4920

ctggaaacca actataccct gtatgtgggc aaccgtcaga acatgattgt ggaaccgaac     4980

tatgatctgg atgatagcgg cgatattagc agcaccgtga ttaactttag ccagaaatat     5040

ctgtatggca ttgatagctg cgtgaacaaa gtggtgatta gcccgaacat ttataccgat     5100

gaaattaaca ttaccccggt gtatgaaacc aacaacacct atccggaagt gattgtgctg     5160

gatgcgaact atattaacga aaaaattaac gtgaacatta acgatctgag cattcgttat     5220

gtgtggagca acgatggcaa cgattttatt ctgatgagca ccagcgaaga aaacaaagtg     5280

agccaggtga aaattcgttt tgtgaacgtg tttaaagata aaaccctggc gaacaaactg     5340

agctttaact ttagcgataa acaggatgtg cctgtgagcg aaattattct gagctttacc     5400

ccgagctatt atgaagatgg cctgattggc tatgatctgg gcctggttag cctgtataac     5460

gaaaaatttt atattaacaa ctttggcatg atggttagcg gcctgattta tattaacgat     5520

agcctgtatt attttaaacc gccggtgaac aacctgatta ccggctttgt gaccgtgggc     5580

gatgataaat attattttaa cccgattaac ggcggcgcgg cgagcattgg cgaaaccatt     5640

attgatgata aaaactatta ttttaaccag agcggcgtgc tgcagaccgg cgtgtttagc     5700

accgaagatg gctttaaata ttttgcgccg gcgaacaccc tggatgaaaa cctggaaggc     5760

gaagcgatcg atttcaccgg caaactgatc atcgatgaaa acatctacta cttcgatgat     5820

aactaccgtg gcgcggtgga atggaaagaa ctggatggcg aaatgcatta ttttagcccg     5880

gaaaccggca aagcgtttaa aggcctgaac cagattggcg attataaata ttattttaac     5940

agcgatggcg tgatgcagaa aggctttgtg agcattaacg ataacaaaca ttattttgat     6000

gatagcggcg tgatgaaagt gggctatacc gaaattgatg gcaaacattt ttattttgcg     6060

gaaaacggcg aaatgcagat tggcgtgttt aatacggaag acggttttaa atattttgcg     6120

catcataacg aagatctggg caacgaagaa ggcgaagaaa tcagctacag cggcattctg     6180

aactttaaca acaaaattta ttacttcgat gatagcttta ccgcggtggt gggctggaaa     6240

gatctggaag atggcagcaa atattatttt gatgaagata ccgcggaagc gtatattggc     6300

ctgagcctga ttaacgatgg ccagtattat tttaacgatg atggcattat gcaagtgggc     6360

tttgtgacca ttaacgataa agtgttttat tttagcgata gcggcattat tgaaagcggc     6420

gtgcagaaca ttgatgataa ctatttttat attgatgata acggcattgt gcagattggc     6480

gtgtttgata ccagcgatgg ctacaaatac ttcgccccgg cgaacaccgt gaacgataac     6540

atttatggcc aggcggtgga atatagcggc ctggtgcgtg tgggcgaaga tgtgtattat     6600

tttggcgaaa cctataccat tgaaaccggc tggatctacg atatggaaaa cgaaagcgat     6660

aaatactact tcaacccgga aaccaaaaaa gcgtgcaaag gcatcaacct gatcgatgat     6720

atcaaatact acttcgatga aaaaggcatc atgcgtaccg gcctgattag ctttgaaaac     6780

aacaattact acttcaacga aaatggtgaa atgcagttcg gctacatcaa catcgaagat     6840

aaaatgtttt attttggcga agatggcgtg atgcaaatcg gcgtgtttaa caccccggac     6900

ggtttcaaat attttgcgca tcaaaatacc ctggatgaaa actttgaagg cgaaagcatt     6960

aactataccg gctggctgga tctggatgaa aaacgttatt attttaccga tgaatatatt     7020

gcggcgaccg gcagcgtgat tattgatggc gaagaatatt attttgatcc ggataccgcg     7080

cagctggtga ttagcgaata a                                               7101


<210>  6
<211>  2166
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(2166)

<400>  6
atgctgtatg tgggcaaccg tcagaacatg attgtggaac cgaactatga tctggatgat       60

agcggcgata ttagcagcac cgtgattaac tttagccaga aatatctgta tggcattgat      120

agctgcgtga acaaagtggt gattagcccg aacatttata ccgatgaaat taacattacc      180

ccggtgtatg aaaccaacaa cacctatccg gaagtgattg tgctggatgc gaactatatt      240

aacgaaaaaa ttaacgtgaa cattaacgat ctgagcattc gttatgtgtg gagcaacgat      300

ggcaacgatt ttattctgat gagcaccagc gaagaaaaca aagtgagcca ggtgaaaatt      360

cgttttgtga acgtgtttaa agataaaacc ctggcgaaca aactgagctt taactttagc      420

gataaacagg atgtgcctgt gagcgaaatt attctgagct ttaccccgag ctattatgaa      480

gatggcctga ttggctatga tctgggcctg gttagcctgt ataacgaaaa attttatatt      540

aacaactttg gcatgatggt tagcggcctg atttatatta acgatagcct gtattatttt      600

aaaccgccgg tgaacaacct gattaccggc tttgtgaccg tgggcgatga taaatattat      660

tttaacccga ttaacggcgg cgcggcgagc attggcgaaa ccattattga tgataaaaac      720

tattatttta accagagcgg cgtgctgcag accggcgtgt ttagcaccga agatggcttt      780

aaatattttg cgccggcgaa caccctggat gaaaacctgg aaggcgaagc gatcgatttc      840

accggcaaac tgatcatcga tgaaaacatc tactacttcg atgataacta ccgtggcgcg      900

gtggaatgga aagaactgga tggcgaaatg cattatttta gcccggaaac cggcaaagcg      960

tttaaaggcc tgaaccagat tggcgattat aaatattatt ttaacagcga tggcgtgatg     1020

cagaaaggct ttgtgagcat taacgataac aaacattatt ttgatgatag cggcgtgatg     1080

aaagtgggct ataccgaaat tgatggcaaa catttttatt ttgcggaaaa cggcgaaatg     1140

cagattggcg tgtttaatac ggaagacggt tttaaatatt ttgcgcatca taacgaagat     1200

ctgggcaacg aagaaggcga agaaatcagc tacagcggca ttctgaactt taacaacaaa     1260

atttattact tcgatgatag ctttaccgcg gtggtgggct ggaaagatct ggaagatggc     1320

agcaaatatt attttgatga agataccgcg gaagcgtata ttggcctgag cctgattaac     1380

gatggccagt attattttaa cgatgatggc attatgcaag tgggctttgt gaccattaac     1440

gataaagtgt tttattttag cgatagcggc attattgaaa gcggcgtgca gaacattgat     1500

gataactatt tttatattga tgataacggc attgtgcaga ttggcgtgtt tgataccagc     1560

gatggctaca aatacttcgc cccggcgaac accgtgaacg ataacattta tggccaggcg     1620

gtggaatata gcggcctggt gcgtgtgggc gaagatgtgt attattttgg cgaaacctat     1680

accattgaaa ccggctggat ctacgatatg gaaaacgaaa gcgataaata ctacttcaac     1740

ccggaaacca aaaaagcgtg caaaggcatc aacctgatcg atgatatcaa atactacttc     1800

gatgaaaaag gcatcatgcg taccggcctg attagctttg aaaacaacaa ttactacttc     1860

aacgaaaatg gtgaaatgca gttcggctac atcaacatcg aagataaaat gttttatttt     1920

ggcgaagatg gcgtgatgca aatcggcgtg tttaacaccc cggacggttt caaatatttt     1980

gcgcatcaaa ataccctgga tgaaaacttt gaaggcgaaa gcattaacta taccggctgg     2040

ctggatctgg atgaaaaacg ttattatttt accgatgaat atattgcggc gaccggcagc     2100

gtgattattg atggcgaaga atattatttt gatccggata ccgcgcagct ggtgattagc     2160

gaataa                                                                2166


<210>  7
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  7
atgagcctgg tgaaccgtaa acagctggaa aaaatggcga acgtgcgttt tcgtacccag       60

gaagatgaat atgtggcgat tctggatgcg ctggaagaat atcataacat gagcgaaaac      120

accgtggtgg aaaaatacct gaaactgaaa gatatcaaca gcctgaccga tatctacatc      180

gatacctaca agaaaagcgg ccgtaacaaa gcgctgaaaa aatttaaaga atatctggtg      240

accgaagtgc tggaactgaa aaacaacaac ctgaccccgg tggaaaagaa cctgcatttt      300

gtggcgattg gcggccagat taacgatacc gcgattaact atattaacca gtggaaagat      360

gtgaacagcg attataacgt gaacgtgttt tatgatagca acgcgtttct gattaacacc      420

ctgaagaaaa ccgtggtgga aagcgcgatt aacgataccc tggaaagctt tcgtgaaaac      480

ctgaacgatc cgcgtttcga ttacaacaaa tttttccgta aacgtatgga aatcatctac      540

gataaacaga aaaactttat taactattat aaagcgcagc gtgaagaaaa cccggaactg      600

atcatcgatg atatcgtgaa aacctacctg agcaacgaat acagcaaaga aatcgatgaa      660

ctgaacacct acatcgaaga aagcctgaac aaaatcaccc agaacagcgg caacgatgtg      720

cgtaactttg aagaatttaa aaacggcgaa agctttaacc tgtatgaaca ggaactggtg      780

gaacgttgga acctggcggc ggcgagcgat attctgcgta ttagcgcgct gaaagaaatt      840

ggcggcatgt atctggatgt ggatatgctg ccgggcattc agccggatct gtttgaaagc      900

attgaaaaac cgagcagcgt gaccgtggat ttttgggaaa tgaccaaact ggaagcgatc      960

atgaaataca aagaatacat cccggaatac accagcgaac atttcgatat gctggatgaa     1020

gaagtgcaga gcagctttga aagcgtgctg gcgagcaaaa gcgataaaag cgaaattttt     1080

agcagcctgg gcgatatgga agcgagcccg ctggaagtga aaattgcgtt taacagcaaa     1140

ggcattatta accagggcct gattagcgtg aaagatagct attgcagcaa cctgattgtg     1200

aaacagattg aaaaccgtta taaaattctg aacaacagcc tgaacccggc gattagcgaa     1260

gataacgatt ttaacaccac caccaacacc tttattgata gcattatggc ggaagcgaac     1320

gcggataacg gccgttttat gatggaactg ggcaaatatc tgcgtgtggg tttctttccg     1380

gatgtgaaaa ccaccattaa cctgagcggc ccggaagcgt atgcggcggc gtatcaggat     1440

ctgctgatgt ttaaagaagg cagcatgaac attcatctga ttgaagcgga tctgcgtaac     1500

tttgaaatta gcaaaaccaa cattagccag agcaccgaac aggaaatggc gagcctgtgg     1560

agctttgatg atgcgcgtgc gaaagcgcag tttgaagaat ataaacgtaa ctattttgaa     1620

ggcagcctgg gcgaagatga taacctggat tttagccaga acattgtggt ggataaagaa     1680

tatctgctgg aaaaaattag cagcctggcg cgtagcagcg aacgtggcta tattcattat     1740

attgtgcagc tgcagggcga taaaattagc tatgaagcgg cgtgcaacct gtttgcgaaa     1800

accccgtatg atagcgtgct gtttcagaaa aacattgaag atagcgaaat tgcgtattat     1860

tataacccgg gcgatggcga aatccaggaa atcgataaat acaaaatccc gagcatcatc     1920

agcgatcgtc cgaaaatcaa actgaccttt attggccatg gcaaagatga atttaacacc     1980

gatatttttg cgggctttga tgtggatagc ctgagcaccg aaatcgaagc ggcgattgat     2040

ctggcgaaag aagatattag cccgaaaagc attgaaatta acctgctggg ctgcaacatg     2100

tttagctata gcattaacgt ggaagaaacc tatccgggca aactgctgct gaaagtgaaa     2160

gataaaatta gcgaactgat gccgagcatt agccaggata gcattattgt gagcgcgaac     2220

cagtatgaag tgcgtattaa cagcgaaggc cgtcgtgaac tgctggatca tagcggcgaa     2280

tggatcaaca aagaagaaag catcatcaaa gatatcagca gcaaagaata catcagcttc     2340

aacccgaaag aaaacaaaat caccgtgaaa agcaaaaacc tgccggaact gagcaccctg     2400

ctgcaggaaa ttcgtaacaa cagcaacagc agcgatattg aactggaaga aaaagtgatg     2460

ctgaccgaat gcgaaattaa cgtgattagc aacattgata cccagattgt ggaagaacgt     2520

attgaagaag cgaaaaacct gaccagcgat agcattaact atattaaaga tgaatttaaa     2580

ctgattgaaa gcattagcga tgcgctgtgc gatctgaaac agcagaacga actggaagac     2640

agccatttta ttagctttga agatattagc gaaaccgatg aaggctttag cattcgtttt     2700

attaacaaag aaaccggcga aagcattttt gtggaaaccg aaaaaaccat ttttagcgaa     2760

tacgcgaacc atatcaccga agaaatcagc aaaatcaaag gcaccatttt tgataccgtg     2820

aacggcaaac tggtgaaaaa agtgaacctg gataccaccc atgaagtgaa caccctgaac     2880

gcggcatttt tcattcagag cctgattgaa tataacagca gcaaagaaag cctgagcaac     2940

ctgagcgtgg cgatgaaagt gcaggtgtat gcgcagctgt ttagcaccgg cctgaacacc     3000

attaccgacg cggcgaaagt ggtggaactg gttagcaccg cgctggatga aaccattgat     3060

ctgctgccga ccctgagcga aggcctgccg attattgcga ccattattga tggcgtgagc     3120

ctgggcgcgg cgattaaaga actgagcgaa accagcgatc cgctgctgcg tcaggaaatt     3180

gaagcgaaaa ttggcattat ggcggtgaac ctgaccaccg cgaccaccgc gattattacc     3240

agcagcctgg gcattgcgag cggctttagc attctgctgg tgccgctggc gggcattagc     3300

gcgggcattc cgagcctggt gaacaacgaa ctggtgctgc gtgataaagc gaccaaagtg     3360

gtggattatt ttaaacatgt gagcctggtg gaaaccgaag gcgtgtttac cctgctggat     3420

gataaaatta tgatgccgca ggatgatctg gtgattagcg aaattgattt taacaacaac     3480

agcattgtgc tgggcaaatg cgaaatttgg cgtatggaag gcggcagcgg ccataccgtg     3540

accgatgata ttgatcattt ctttagcgcg ccgagcatta cctatcgtga accgcatctg     3600

agcatttatg atgtgctgga agtgcagaaa gaagaactgg atctgagcaa agatctgatg     3660

gtgctgccga acgcaccgaa ccgtgtgttt gcatgggaaa ccggttggac cccgggtctg     3720

cgtagcctgg aaaacgatgg caccaaactg ctggatcgta ttcgtgataa ctatgaaggc     3780

gaattttatt ggcgttattt tgcgtttatt gcggatgcgc tgattaccac cctgaaaccg     3840

cgttatgaag ataccaacat tcgtattaac ctggatagca acacccgtag ctttattgtg     3900

ccgattatta ccaccgaata tattcgtgaa aaactgagct atagctttta tggcagcggc     3960

ggcacctatg cgctgagcct gagccagtat aacatgggca ttaacattga actgagcgaa     4020

agcgatgtgt ggattattga tgtggataac gtggtgcgtg atgtgaccat tgaaagcgat     4080

aaaattaaaa aaggcgatct gattgaaggc attctgagca ccctgagcat tgaagaaaac     4140

aaaattattc tgaacagcca tgaaattaac tttagcggcg aagtgaacgg cagcaacggc     4200

tttgtgagcc tgacctttag cattctggaa ggcattaacg cgattattga agtggatctg     4260

ctgagcaaaa gctataaact gctgattagc ggcgaactga aaattctgat gctgaacagc     4320

aaccatattc agcagaaaat tgattatatt ggctttaaca gcgaactgca gaaaaacatt     4380

ccgtatagct ttgtggatag cgaaggcaaa gaaaacggct ttattaacgg cagcaccaaa     4440

gaaggcctgt ttgtgagcga actgccggat gtggtgctga ttagcaaagt gtatatggat     4500

gatagcaaac cgagctttgg ctattatagc aacaacctga aagatgtgaa agtgattacc     4560

aaagataacg tgaacattct gaccggctat tatctgaaag atgatattaa aattagcctg     4620

agcctgaccc tgcaggatga aaaaaccatt aaactgaaca gcgtgcatct ggatgaaagc     4680

ggcgtggcgg aaattctgaa atttatgaac cgtaaaggca acaccaacac cagcgatagc     4740

ctgatgagct ttctggaaag catgaacatc aaaagcatct tcgtgaactt tctgcagagc     4800

aacatcaaat tcatcctgga tgcgaacttc atcatcagcg gcaccaccag cattggccag     4860

ttcgaattta tttgcgatga aaacgataac atccagccgt acttcatcaa attcaacacc     4920

ctggaaacca actataccct gtatgtgggc aaccgtcaga acatgattgt ggaaccgaac     4980

tatgatctgg atgatagcgg cgatattagc agcaccgtga ttaactttag ccagaaatat     5040

ctgtatggca ttgatagctg cgtgaacaaa gtggtgatta gcccgaacat ttataccgat     5100

gaaattaaca ttaccccggt gtatgaaacc aacaacacct atccggaagt gattgtgctg     5160

gatgcgaact atattaacga aaaaattaac gtgaacatta acgatctgag cattcgttat     5220

gtgtggagca acgatggcaa cgattttatt ctgatgagca ccagcgaaga aaacaaagtg     5280

agccaggtga aaattcgttt tgtgaacgtg tttaaagata aaaccctggc gaacaaactg     5340

agctttaact ttagcgataa acaggatgtg cctgtgagcg aaattattct gagctttacc     5400

ccgagctatt atgaagatgg cctgattggc tatgatctgg gcctggttag cctgtataac     5460

gaaaaatttt atattaacaa ctttggcatg atggttagcg gcctgattta tattaacgat     5520

agcctgtatt attttaaacc gccggtgaac aacctgatta ccggctttgt gaccgtgggc     5580

gatgataaat attattttaa cccgattaac ggcggcgcgg cgagcattgg cgaaaccatt     5640

attgatgata aaaactatta ttttaaccag agcggcgtgc tgcagaccgg cgtgtttagc     5700

accgaagatg gctttaaata ttttgcgccg gcgaacaccc tggatgaaaa cctggaaggc     5760

gaagcgatcg atttcaccgg caaactgatc atcgatgaaa acatctacta cttcgatgat     5820

aactaccgtg gcgcggtgga atggaaagaa ctggatggcg aaatgcatta ttttagcccg     5880

gaaaccggca aagcgtttaa aggcctgaac cagattggcg attataaata ttattttaac     5940

agcgatggcg tgatgcagaa aggctttgtg agcattaacg ataacaaaca ttattttgat     6000

gatagcggcg tgatgaaagt gggctatacc gaaattgatg gcaaacattt ttattttgcg     6060

gaaaacggcg aaatgcagat tggcgtgttt aatacggaag acggttttaa atattttgcg     6120

catcataacg aagatctggg caacgaagaa ggcgaagaaa tcagctacag cggcattctg     6180

aactttaaca acaaaattta ttacttcgat gatagcttta ccgcggtggt gggctggaaa     6240

gatctggaag atggcagcaa atattatttt gatgaagata ccgcggaagc gtatattggc     6300

ctgagcctga ttaacgatgg ccagtattat tttaacgatg atggcattat gcaagtgggc     6360

tttgtgacca ttaacgataa agtgttttat tttagcgata gcggcattat tgaaagcggc     6420

gtgcagaaca ttgatgataa ctatttttat attgatgata acggcattgt gcagattggc     6480

gtgtttgata ccagcgatgg ctacaaatac ttcgccccgg cgaacaccgt gaacgataac     6540

atttatggcc aggcggtgga atatagcggc ctggtgcgtg tgggcgaaga tgtgtattat     6600

tttggcgaaa cctataccat tgaaaccggc tggatctacg atatggaaaa cgaaagcgat     6660

aaatactact tcaacccgga aaccaaaaaa gcgtgcaaag gcatcaacct gatcgatgat     6720

atcaaatact acttcgatga aaaaggcatc atgcgtaccg gcctgattag ctttgaaaac     6780

aacaattact acttcaacga aaatggtgaa atgcagttcg gctacatcaa catcgaagat     6840

aaaatgtttt attttggcga agatggcgtg atgcaaatcg gcgtgtttaa caccccggac     6900

ggtttcaaat attttgcgca tcaaaatacc ctggatgaaa actttgaagg cgaaagcatt     6960

aactataccg gctggctgga tctggatgaa aaacgttatt attttaccga tgaatatatt     7020

gcggcgaccg gcagcgtgat tattgatggc gaagaatatt attttgatcc ggataccgcg     7080

cagctggtga ttagcgaata a                                               7101


<210>  8
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  8
atgagcctgg tgaaccgtaa acagctggaa aaaatggcga acgtgcgttt tcgtacccag       60

gaagatgaat atgtggcgat tctggatgcg ctggaagaat atcataacat gagcgaaaac      120

accgtggtgg aaaaatacct gaaactgaaa gatatcaaca gcctgaccga tatctacatc      180

gatacctaca agaaaagcgg ccgtaacaaa gcgctgaaaa aatttaaaga atatctggtg      240

accgaagtgc tggaactgaa aaacaacaac ctgaccccgg tggaaaagaa cctgcatttt      300

gtgtggattg gcggccagat taacgatacc gcgattaact atattaacca gtggaaagat      360

gtgaacagcg attataacgt gaacgtgttt tatgatagca acgcgtttct gattaacacc      420

ctgaagaaaa ccgtggtgga aagcgcgatt aacgataccc tggaaagctt tcgtgaaaac      480

ctgaacgatc cgcgtttcga ttacaacaaa tttttccgta aacgtatgga aatcatctac      540

gataaacaga aaaactttat taactattat aaagcgcagc gtgaagaaaa cccggaactg      600

atcatcgatg atatcgtgaa aacctacctg agcaacgaat acagcaaaga aatcgatgaa      660

ctgaacacct acatcgaaga aagcctgaac aaaatcaccc agaacagcgg caacgatgtg      720

cgtaactttg aagaatttaa aaacggcgaa agctttaacc tgtatgaaca ggaactggtg      780

gaacgttgga acctggcggc ggcgagcgat attctgcgta ttagcgcgct gaaagaaatt      840

ggcggcatgt atctgggtgt gggtatgctg ccgggcattc agccggatct gtttgaaagc      900

attgaaaaac cgagcagcgt gaccgtggat ttttgggaaa tgaccaaact ggaagcgatc      960

atgaaataca aagaatacat cccggaatac accagcgaac atttcgatat gctggatgaa     1020

gaagtgcaga gcagctttga aagcgtgctg gcgagcaaaa gcgataaaag cgaaattttt     1080

agcagcctgg gcgatatgga agcgagcccg ctggaagtga aaattgcgtt taacagcaaa     1140

ggcattatta accagggcct gattagcgtg aaagatagct attgcagcaa cctgattgtg     1200

aaacagattg aaaaccgtta taaaattctg aacaacagcc tgaacccggc gattagcgaa     1260

gataacgatt ttaacaccac caccaacacc tttattgata gcattatggc ggaagcgaac     1320

gcggataacg gccgttttat gatggaactg ggcaaatatc tgcgtgtggg tttctttccg     1380

gatgtgaaaa ccaccattaa cctgagcggc ccggaagcgt atgcggcggc gtatcaggat     1440

ctgctgatgt ttaaagaagg cagcatgaac attcatctga ttgaagcgga tctgcgtaac     1500

tttgaaatta gcaaaaccaa cattagccag agcaccgaac aggaaatggc gagcctgtgg     1560

agctttgatg atgcgcgtgc gaaagcgcag tttgaagaat ataaacgtaa ctattttgaa     1620

ggcagcctgg gcgaagatga taacctggat tttagccaga acattgtggt ggataaagaa     1680

tatctgctgg aaaaaattag cagcctggcg cgtagcagcg aacgtggcta tattcattat     1740

attgtgcagc tgcagggcga taaaattagc tatgaagcgg cgtgcaacct gtttgcgaaa     1800

accccgtatg atagcgtgct gtttcagaaa aacattgaag atagcgaaat tgcgtattat     1860

tataacccgg gcgatggcga aatccaggaa atcgataaat acaaaatccc gagcatcatc     1920

agcgatcgtc cgaaaatcaa actgaccttt attggccatg gcaaagatga atttaacacc     1980

gatatttttg cgggctttga tgtggatagc ctgagcaccg aaatcgaagc ggcgattgat     2040

ctggcgaaag aagatattag cccgaaaagc attgaaatta acctgctggg ctgcaacatg     2100

tttagctata gcattaacgt ggaagaaacc tatccgggca aactgctgct gaaagtgaaa     2160

gataaaatta gcgaactgat gccgagcatt agccaggata gcattattgt gagcgcgaac     2220

cagtatgaag tgcgtattaa cagcgaaggc cgtcgtgaac tgctggatca tagcggcgaa     2280

tggatcaaca aagaagaaag catcatcaaa gatatcagca gcaaagaata catcagcttc     2340

aacccgaaag aaaacaaaat caccgtgaaa agcaaaaacc tgccggaact gagcaccctg     2400

ctgcaggaaa ttcgtaacaa cagcaacagc agcgatattg aactggaaga aaaagtgatg     2460

ctgaccgaat gcgaaattaa cgtgattagc aacattgata cccagattgt ggaagaacgt     2520

attgaagaag cgaaaaacct gaccagcgat agcattaact atattaaaga tgaatttaaa     2580

ctgattgaaa gcattagcga tgcgctgtgc gatctgaaac agcagaacga actggaagac     2640

agccatttta ttagctttga agatattagc gaaaccgatg aaggctttag cattcgtttt     2700

attaacaaag aaaccggcga aagcattttt gtggaaaccg aaaaaaccat ttttagcgaa     2760

tacgcgaacc atatcaccga agaaatcagc aaaatcaaag gcaccatttt tgataccgtg     2820

aacggcaaac tggtgaaaaa agtgaacctg gataccaccc atgaagtgaa caccctgaac     2880

gcggcatttt tcattcagag cctgattgaa tataacagca gcaaagaaag cctgagcaac     2940

ctgagcgtgg cgatgaaagt gcaggtgtat gcgcagctgt ttagcaccgg cctgaacacc     3000

attaccgacg cggcgaaagt ggtggaactg gttagcaccg cgctggatga aaccattgat     3060

ctgctgccga ccctgagcga aggcctgccg attattgcga ccattattga tggcgtgagc     3120

ctgggcgcgg cgattaaaga actgagcgaa accagcgatc cgctgctgcg tcaggaaatt     3180

gaagcgaaaa ttggcattat ggcggtgaac ctgaccaccg cgaccaccgc gattattacc     3240

agcagcctgg gcattgcgag cggctttagc attctgctgg tgccgctggc gggcattagc     3300

gcgggcattc cgagcctggt gaacaacgaa ctggtgctgc gtgataaagc gaccaaagtg     3360

gtggattatt ttaaacatgt gagcctggtg gaaaccgaag gcgtgtttac cctgctggat     3420

gataaaatta tgatgccgca ggatgatctg gtgattagcg aaattgattt taacaacaac     3480

agcattgtgc tgggcaaatg cgaaatttgg cgtatggaag gcggcagcgg ccataccgtg     3540

accgatgata ttgatcattt ctttagcgcg ccgagcatta cctatcgtga accgcatctg     3600

agcatttatg atgtgctgga agtgcagaaa gaagaactgg atctgagcaa agatctgatg     3660

gtgctgccga acgcaccgaa ccgtgtgttt gcatgggaaa ccggttggac cccgggtctg     3720

cgtagcctgg aaaacgatgg caccaaactg ctggatcgta ttcgtgataa ctatgaaggc     3780

gaattttatt ggcgttattt tgcgtttatt gcggatgcgc tgattaccac cctgaaaccg     3840

cgttatgaag ataccaacat tcgtattaac ctggatagca acacccgtag ctttattgtg     3900

ccgattatta ccaccgaata tattcgtgaa aaactgagct atagctttta tggcagcggc     3960

ggcacctatg cgctgagcct gagccagtat aacatgggca ttaacattga actgagcgaa     4020

agcgatgtgt ggattattga tgtggataac gtggtgcgtg atgtgaccat tgaaagcgat     4080

aaaattaaaa aaggcgatct gattgaaggc attctgagca ccctgagcat tgaagaaaac     4140

aaaattattc tgaacagcca tgaaattaac tttagcggcg aagtgaacgg cagcaacggc     4200

tttgtgagcc tgacctttag cattctggaa ggcattaacg cgattattga agtggatctg     4260

ctgagcaaaa gctataaact gctgattagc ggcgaactga aaattctgat gctgaacagc     4320

aaccatattc agcagaaaat tgattatatt ggctttaaca gcgaactgca gaaaaacatt     4380

ccgtatagct ttgtggatag cgaaggcaaa gaaaacggct ttattaacgg cagcaccaaa     4440

gaaggcctgt ttgtgagcga actgccggat gtggtgctga ttagcaaagt gtatatggat     4500

gatagcaaac cgagctttgg ctattatagc aacaacctga aagatgtgaa agtgattacc     4560

aaagataacg tgaacattct gaccggctat tatctgaaag atgatattaa aattagcctg     4620

agcctgaccc tgcaggatga aaaaaccatt aaactgaaca gcgtgcatct ggatgaaagc     4680

ggcgtggcgg aaattctgaa atttatgaac cgtaaaggca acaccaacac cagcgatagc     4740

ctgatgagct ttctggaaag catgaacatc aaaagcatct tcgtgaactt tctgcagagc     4800

aacatcaaat tcatcctgga tgcgaacttc atcatcagcg gcaccaccag cattggccag     4860

ttcgaattta tttgcgatga aaacgataac atccagccgt acttcatcaa attcaacacc     4920

ctggaaacca actataccct gtatgtgggc aaccgtcaga acatgattgt ggaaccgaac     4980

tatgatctgg atgatagcgg cgatattagc agcaccgtga ttaactttag ccagaaatat     5040

ctgtatggca ttgatagctg cgtgaacaaa gtggtgatta gcccgaacat ttataccgat     5100

gaaattaaca ttaccccggt gtatgaaacc aacaacacct atccggaagt gattgtgctg     5160

gatgcgaact atattaacga aaaaattaac gtgaacatta acgatctgag cattcgttat     5220

gtgtggagca acgatggcaa cgattttatt ctgatgagca ccagcgaaga aaacaaagtg     5280

agccaggtga aaattcgttt tgtgaacgtg tttaaagata aaaccctggc gaacaaactg     5340

agctttaact ttagcgataa acaggatgtg cctgtgagcg aaattattct gagctttacc     5400

ccgagctatt atgaagatgg cctgattggc tatgatctgg gcctggttag cctgtataac     5460

gaaaaatttt atattaacaa ctttggcatg atggttagcg gcctgattta tattaacgat     5520

agcctgtatt attttaaacc gccggtgaac aacctgatta ccggctttgt gaccgtgggc     5580

gatgataaat attattttaa cccgattaac ggcggcgcgg cgagcattgg cgaaaccatt     5640

attgatgata aaaactatta ttttaaccag agcggcgtgc tgcagaccgg cgtgtttagc     5700

accgaagatg gctttaaata ttttgcgccg gcgaacaccc tggatgaaaa cctggaaggc     5760

gaagcgatcg atttcaccgg caaactgatc atcgatgaaa acatctacta cttcgatgat     5820

aactaccgtg gcgcggtgga atggaaagaa ctggatggcg aaatgcatta ttttagcccg     5880

gaaaccggca aagcgtttaa aggcctgaac cagattggcg attataaata ttattttaac     5940

agcgatggcg tgatgcagaa aggctttgtg agcattaacg ataacaaaca ttattttgat     6000

gatagcggcg tgatgaaagt gggctatacc gaaattgatg gcaaacattt ttattttgcg     6060

gaaaacggcg aaatgcagat tggcgtgttt aatacggaag acggttttaa atattttgcg     6120

catcataacg aagatctggg caacgaagaa ggcgaagaaa tcagctacag cggcattctg     6180

aactttaaca acaaaattta ttacttcgat gatagcttta ccgcggtggt gggctggaaa     6240

gatctggaag atggcagcaa atattatttt gatgaagata ccgcggaagc gtatattggc     6300

ctgagcctga ttaacgatgg ccagtattat tttaacgatg atggcattat gcaagtgggc     6360

tttgtgacca ttaacgataa agtgttttat tttagcgata gcggcattat tgaaagcggc     6420

gtgcagaaca ttgatgataa ctatttttat attgatgata acggcattgt gcagattggc     6480

gtgtttgata ccagcgatgg ctacaaatac ttcgccccgg cgaacaccgt gaacgataac     6540

atttatggcc aggcggtgga atatagcggc ctggtgcgtg tgggcgaaga tgtgtattat     6600

tttggcgaaa cctataccat tgaaaccggc tggatctacg atatggaaaa cgaaagcgat     6660

aaatactact tcaacccgga aaccaaaaaa gcgtgcaaag gcatcaacct gatcgatgat     6720

atcaaatact acttcgatga aaaaggcatc atgcgtaccg gcctgattag ctttgaaaac     6780

aacaattact acttcaacga aaatggtgaa atgcagttcg gctacatcaa catcgaagat     6840

aaaatgtttt attttggcga agatggcgtg atgcaaatcg gcgtgtttaa caccccggac     6900

ggtttcaaat attttgcgca tcaaaatacc ctggatgaaa actttgaagg cgaaagcatt     6960

aactataccg gctggctgga tctggatgaa aaacgttatt attttaccga tgaatatatt     7020

gcggcgaccg gcagcgtgat tattgatggc gaagaatatt attttgatcc ggataccgcg     7080

cagctggtga ttagcgaata a                                               7101


<210>  9
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  9
atgagcctgg tgaaccgtaa acagctggaa aaaatggcga acgtgcgttt tcgtacccag       60

gaagatgaat atgtggcgat tctggatgcg ctggaagaat atcataacat gagcgaaaac      120

accgtggtgg aaaaatacct gaaactgaaa gatatcaaca gcctgaccga tatctacatc      180

gatacctaca agaaaagcgg ccgtaacaaa gcgctgaaaa aatttaaaga atatctggtg      240

accgaagtgc tggaactgaa aaacaacaac ctgaccccgg tggaaaagaa cctgcatttt      300

gtggcgattg gcggccagat taacgatacc gcgattaact atattaacca gtggaaagat      360

gtgaacagcg attataacgt gaacgtgttt tatgatagca acgcgtttct gattaacacc      420

ctgaagaaaa ccgtggtgga aagcgcgatt aacgataccc tggaaagctt tcgtgaaaac      480

ctgaacgatc cgcgtttcga ttacaacaaa tttttccgta aacgtatgga aatcatctac      540

gataaacaga aaaactttat taactattat aaagcgcagc gtgaagaaaa cccggaactg      600

atcatcgatg atatcgtgaa aacctacctg agcaacgaat acagcaaaga aatcgatgaa      660

ctgaacacct acatcgaaga aagcctgaac aaaatcaccc agaacagcgg caacgatgtg      720

cgtaactttg aagaatttaa aaacggcgaa agctttaacc tgtatgaaca ggaactggtg      780

gaacgttgga acctggcggc ggcgagcgat attctgcgta ttagcgcgct gaaagaaatt      840

ggcggcatgt atctggatgt ggatatgctg ccgggcattc agccggatct gtttgaaagc      900

attgaaaaac cgagcagcgt gaccgtggat ttttgggaaa tgaccaaact ggaagcgatc      960

atgaaataca aagaatacat cccggaatac accagcgaac atttcgatat gctggatgaa     1020

gaagtgcaga gcagctttga aagcgtgctg gcgagcaaaa gcgataaaag cgaaattttt     1080

agcagcctgg gcgatatgga agcgagcccg ctggaagtga aaattgcgtt taacagcaaa     1140

ggcattatta accagggcct gattagcgtg aaagatagct attgcagcaa cctgattgtg     1200

aaacagattg aaaaccgtta taaaattctg aacaacagcc tgaacccggc gattagcgaa     1260

gataacgatt ttaacaccac caccaacacc tttattgata gcattatggc ggaagcgaac     1320

gcggataacg gccgttttat gatggaactg ggcaaatatc tgcgtgtggg tttctttccg     1380

gatgtgaaaa ccaccattaa cctgagcggc ccggaagcgt atgcggcggc gtatcaggat     1440

ctgctgatgt ttaaagaagg cagcatgaac attcatctga ttgaagcgga tctgcgtaac     1500

tttgaaatta gcaaaaccaa cattagccag agcaccgaac aggaaatggc gagcctgtgg     1560

agctttgatg atgcgcgtgc gaaagcgcag tttgaagaat ataaacgtaa ctattttgaa     1620

ggcagcctgg gcgaagatga taacctggat tttagccaga acattgtggt ggataaagaa     1680

tatctgctgg aaaaaattag cagcctggcg cgtagcagcg aacgtggcta tattcattat     1740

attgtgcagc tgcagggcga taaaattagc tatgaagcgg cgtgcaacct gtttgcgaaa     1800

accccgtatg atagcgtgct gtttcagaaa aacattgaag atagcgaaat tgcgtattat     1860

tataacccgg gcgatggcga aatccaggaa atcgataaat acaaaatccc gagcatcatc     1920

agcgatcgtc cgaaaatcaa actgaccttt attggccatg gcaaagatga atttaacacc     1980

gatatttttg cgggctttga tgtggatagc ctgagcaccg aaatcgaagc ggcgattgat     2040

ctggcgaaag aagatattag cccgaaaagc attgaaatta acctgctggg ctgcaacatg     2100

tttagctata gcattaacgt ggaagaaacc tatccgggca aactgctgct gaaagtgaaa     2160

gataaaatta gcgaactgat gccgagcatt agccaggata gcattattgt gagcgcgaac     2220

cagtatgaag tgcgtattaa cagcgaaggc cgtcgtgaac tgctggatca tagcggcgaa     2280

tggatcaaca aagaagaaag catcatcaaa gatatcagca gcaaagaata catcagcttc     2340

aacccgaaag aaaacaaaat caccgtgaaa agcaaaaacc tgccggaact gagcaccctg     2400

ctgcaggaaa ttcgtaacaa cagcaacagc agcgatattg aactggaaga aaaagtgatg     2460

ctgaccgaat gcgaaattaa cgtgattagc aacattgata cccagattgt ggaagaacgt     2520

attgaagaag cgaaaaacct gaccagcgat agcattaact atattaaaga tgaatttaaa     2580

ctgattgaaa gcattagcga tgcgctgtgc gatctgaaac agcagaacga actggaagac     2640

agccatttta ttagctttga agatattagc gaaaccgatg aaggctttag cattcgtttt     2700

attaacaaag aaaccggcga aagcattttt gtggaaaccg aaaaaaccat ttttagcgaa     2760

tacgcgaacc atatcaccga agaaatcagc aaaatcaaag gcaccatttt tgataccgtg     2820

aacggcaaac tggtgaaaaa agtgaacctg gataccaccc atgaagtgaa caccctgaac     2880

gcggcatttt tcattcagag cctgattgaa tataacagca gcaaagaaag cctgagcaac     2940

ctgagcgtgg cgatgaaagt gcaggtgtat gcgcagctgt ttagcaccgg cctgaacacc     3000

attaccgacg cggcgaaagt ggtggaactg gttagcaccg cgctggatga aaccattgat     3060

ctgctgccga ccctgagcga aggcctgccg attattgcga ccattattga tggcgtgagc     3120

ctgggcgcgg cgattaaaga actgagcgaa accagcgatc cgctgctgcg tcaggaaatt     3180

gaagcgaaaa ttggcattat ggcggtgaac ctgaccaccg cgaccaccgc gattattacc     3240

agcagcctgg gcattgcgag cggctttagc attctgctgg tgccgctggc gggcattagc     3300

gcgggcattc cgagcctggt gaacaacgaa ctggtgctgc gtgataaagc gaccaaagtg     3360

gtggattatt ttaaacatgt gagcctggtg gaaaccgaag gcgtgtttac cctgctggat     3420

gataaaatta tgatgccgca ggatgatctg gtgattagcg aaattgattt taacaacaac     3480

agcattgtgc tgggcaaatg cgaaatttgg cgtatggaag gcggcagcgg ccataccgtg     3540

accgatgata ttgatcattt ctttagcgcg ccgagcatta cctatcgtga accgcatctg     3600

agcatttatg atgtgctgga agtgcagaaa gaagaactgg atctgagcaa agatctgatg     3660

gtgctgccga acgcaccgaa ccgtgtgttt gcatgggaaa ccggttggac cccgggtctg     3720

cgtagcctgg aaaacgatgg caccaaactg ctggatcgta ttcgtgataa ctatgaaggc     3780

gaattttatt ggcgttattt tgcgtttatt gcggatgcgc tgattaccac cctgaaaccg     3840

cgttatgaag ataccaacat tcgtattaac ctggatagca acacccgtag ctttattgtg     3900

ccgattatta ccaccgaata tattcgtgaa aaactgagct atagctttta tggcagcggc     3960

ggcacctatg cgctgagcct gagccagtat aacatgggca ttaacattga actgagcgaa     4020

agcgatgtgt ggattattga tgtggataac gtggtgcgtg atgtgaccat tgaaagcgat     4080

aaaattaaaa aaggcgatct gattgaaggc attctgagca ccctgagcat tgaagaaaac     4140

aaaattattc tgaacagcca tgaaattaac tttagcggcg aagtgaacgg cagcaacggc     4200

tttgtgagcc tgacctttag cattctggaa ggcattaacg cgattattga agtggatctg     4260

ctgagcaaaa gctataaact gctgattagc ggcgaactga aaattctgat gctgaacagc     4320

aaccatattc agcagaaaat tgattatatt ggctttaaca gcgaactgca gaaaaacatt     4380

ccgtatagct ttgtggatag cgaaggcaaa gaaaacggct ttattaacgg cagcaccaaa     4440

gaaggcctgt ttgtgagcga actgccggat gtggtgctga ttagcaaagt gtatatggat     4500

gatagcaaac cgagctttgg ctattatagc aacaacctga aagatgtgaa agtgattacc     4560

aaagataacg tgaacattct gaccggctat tatctgaaag atgatattaa aattagcctg     4620

agcctgaccc tgcaggatga aaaaaccatt aaactgaaca gcgtgcatct ggatgaaagc     4680

ggcgtggcgg aaattctgaa atttatgaac cgtaaaggca acaccaacac cagcgatagc     4740

ctgatgagct ttctggaaag catgaacatc aaaagcatct tcgtgaactt tctgcagagc     4800

aacatcaaat tcatcctgga tgcgaacttc atcatcagcg gcaccaccag cattggccag     4860

ttcgaattta tttgcgatga aaacgataac atccagccgt acttcatcaa attcaacacc     4920

ctggaaacca actataccct gtatgtgggc aaccgtcaga acatgattgt ggaaccgaac     4980

tatgatctgg atgatagcgg cgatattagc agcaccgtga ttaactttag ccagaaatat     5040

ctgtatggca ttgatagctg cgtgaacaaa gtggtgatta gcccgaacat ttataccgat     5100

gaaattaaca ttaccccggt gtatgaaacc aacaacacct atccggaagt gattgtgctg     5160

gatgcgaact atattaacga aaaaattaac gtgaacatta acgatctgag cattcgttat     5220

gtgtggagca acgatggcaa cgattttatt ctgatgagca ccagcgaaga aaacaaagtg     5280

agccaggtga aaattcgttt tgtgaacgtg tttaaagata aaaccctggc gaacaaactg     5340

agctttaact ttagcgataa acaggatgtg cctgtgagcg aaattattct gagctttacc     5400

ccgagctatt atgaagatgg cctgattggc tatgatctgg gcctggttag cctgtataac     5460

gaaaaatttt atattaacaa ctttggcatg atggttagcg gcctgattta tattaacgat     5520

agcctgtatt attttaaacc gccggtgaac aacctgatta ccggctttgt gaccgtgggc     5580

gatgataaat attattttaa cccgattaac ggcggcgcgg cgagcattgg cgaaaccatt     5640

attgatgata aaaactatta ttttaaccag agcggcgtgc tgcagaccgg cgtgtttagc     5700

accgaagatg gctttaaata ttttgcgccg gcgaacaccc tggatgaaaa cctggaaggc     5760

gaagcgatcg atttcaccgg caaactgatc atcgatgaaa acatctacta cttcgatgat     5820

aactaccgtg gcgcggtgga atggaaagaa ctggatggcg aaatgcatta ttttagcccg     5880

gaaaccggca aagcgtttaa aggcctgaac cagattggcg attataaata ttattttaac     5940

agcgatggcg tgatgcagaa aggctttgtg agcattaacg ataacaaaca ttattttgat     6000

gatagcggcg tgatgaaagt gggctatacc gaaattgatg gcaaacattt ttattttgcg     6060

gaaaacggcg aaatgcagat tggcgtgttt aatacggaag acggttttaa atattttgcg     6120

catcataacg aagatctggg caacgaagaa ggcgaagaaa tcagctacag cggcattctg     6180

aactttaaca acaaaattta ttacttcgat gatagcttta ccgcggtggt gggctggaaa     6240

gatctggaag atggcagcaa atattatttt gatgaagata ccgcggaagc gtatattggc     6300

ctgagcctga ttaacgatgg ccagtattat tttaacgatg atggcattat gcaagtgggc     6360

tttgtgacca ttaacgataa agtgttttat tttagcgata gcggcattat tgaaagcggc     6420

gtgcagaaca ttgatgataa ctatttttat attgatgata acggcattgt gcagattggc     6480

gtgtttgata ccagcgatgg ctacaaatac ttcgccccgg cgaacaccgt gaacgataac     6540

atttatggcc aggcggtgga atatagcggc ctggtgcgtg tgggcgaaga tgtgtattat     6600

tttggcgaaa cctataccat tgaaaccggc tggatctacg atatggaaaa cgaaagcgat     6660

aaatactact tcaacccgga aaccaaaaaa gcgtgcaaag gcatcaacct gatcgatgat     6720

atcaaatact acttcgatga aaaaggcatc atgcgtaccg gcctgattag ctttgaaaac     6780

aacaattact acttcaacga aaatggtgaa atgcagttcg gctacatcaa catcgaagat     6840

aaaatgtttt attttggcga agatggcgtg atgcaaatcg gcgtgtttaa caccccggac     6900

ggtttcaaat attttgcgca tcaaaatacc ctggatgaaa actttgaagg cgaaagcatt     6960

aactataccg gctggctgga tctggatgaa aaacgttatt attttaccga tgaatatatt     7020

gcggcgaccg gcagcgtgat tattgatggc gaagaatatt attttgatcc ggataccgcg     7080

cagctggtga ttagcgaata a                                               7101


<210>  10
<211>  7101
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (1)..(7101)

<400>  10
atgagcctgg tgaaccgtaa acagctggaa aaaatggcga acgtgcgttt tcgtacccag       60

gaagatgaat atgtggcgat tctggatgcg ctggaagaat atcataacat gagcgaaaac      120

accgtggtgg aaaaatacct gaaactgaaa gatatcaaca gcctgaccga tatctacatc      180

gatacctaca agaaaagcgg ccgtaacaaa gcgctgaaaa aatttaaaga atatctggtg      240

accgaagtgc tggaactgaa aaacaacaac ctgaccccgg tggaaaagaa cctgcatttt      300

gtgtggattg gcggccagat taacgatacc gcgattaact atattaacca gtggaaagat      360

gtgaacagcg attataacgt gaacgtgttt tatgatagca acgcgtttct gattaacacc      420

ctgaagaaaa ccgtggtgga aagcgcgatt aacgataccc tggaaagctt tcgtgaaaac      480

ctgaacgatc cgcgtttcga ttacaacaaa tttttccgta aacgtatgga aatcatctac      540

gataaacaga aaaactttat taactattat aaagcgcagc gtgaagaaaa cccggaactg      600

atcatcgatg atatcgtgaa aacctacctg agcaacgaat acagcaaaga aatcgatgaa      660

ctgaacacct acatcgaaga aagcctgaac aaaatcaccc agaacagcgg caacgatgtg      720

cgtaactttg aagaatttaa aaacggcgaa agctttaacc tgtatgaaca ggaactggtg      780

gaacgttgga acctggcggc ggcgagcgat attctgcgta ttagcgcgct gaaagaaatt      840

ggcggcatgt atctgggtgt gggtatgctg ccgggcattc agccggatct gtttgaaagc      900

attgaaaaac cgagcagcgt gaccgtggat ttttgggaaa tgaccaaact ggaagcgatc      960

atgaaataca aagaatacat cccggaatac accagcgaac atttcgatat gctggatgaa     1020

gaagtgcaga gcagctttga aagcgtgctg gcgagcaaaa gcgataaaag cgaaattttt     1080

agcagcctgg gcgatatgga agcgagcccg ctggaagtga aaattgcgtt taacagcaaa     1140

ggcattatta accagggcct gattagcgtg aaagatagct attgcagcaa cctgattgtg     1200

aaacagattg aaaaccgtta taaaattctg aacaacagcc tgaacccggc gattagcgaa     1260

gataacgatt ttaacaccac caccaacacc tttattgata gcattatggc ggaagcgaac     1320

gcggataacg gccgttttat gatggaactg ggcaaatatc tgcgtgtggg tttctttccg     1380

gatgtgaaaa ccaccattaa cctgagcggc ccggaagcgt atgcggcggc gtatcaggat     1440

ctgctgatgt ttaaagaagg cagcatgaac attcatctga ttgaagcgga tctgcgtaac     1500

tttgaaatta gcaaaaccaa cattagccag agcaccgaac aggaaatggc gagcctgtgg     1560

agctttgatg atgcgcgtgc gaaagcgcag tttgaagaat ataaacgtaa ctattttgaa     1620

ggcagcctgg gcgaagatga taacctggat tttagccaga acattgtggt ggataaagaa     1680

tatctgctgg aaaaaattag cagcctggcg cgtagcagcg aacgtggcta tattcattat     1740

attgtgcagc tgcagggcga taaaattagc tatgaagcgg cgtgcaacct gtttgcgaaa     1800

accccgtatg atagcgtgct gtttcagaaa aacattgaag atagcgaaat tgcgtattat     1860

tataacccgg gcgatggcga aatccaggaa atcgataaat acaaaatccc gagcatcatc     1920

agcgatcgtc cgaaaatcaa actgaccttt attggccatg gcaaagatga atttaacacc     1980

gatatttttg cgggctttga tgtggatagc ctgagcaccg aaatcgaagc ggcgattgat     2040

ctggcgaaag aagatattag cccgaaaagc attgaaatta acctgctggg ctgcaacatg     2100

tttagctata gcattaacgt ggaagaaacc tatccgggca aactgctgct gaaagtgaaa     2160

gataaaatta gcgaactgat gccgagcatt agccaggata gcattattgt gagcgcgaac     2220

cagtatgaag tgcgtattaa cagcgaaggc cgtcgtgaac tgctggatca tagcggcgaa     2280

tggatcaaca aagaagaaag catcatcaaa gatatcagca gcaaagaata catcagcttc     2340

aacccgaaag aaaacaaaat caccgtgaaa agcaaaaacc tgccggaact gagcaccctg     2400

ctgcaggaaa ttcgtaacaa cagcaacagc agcgatattg aactggaaga aaaagtgatg     2460

ctgaccgaat gcgaaattaa cgtgattagc aacattgata cccagattgt ggaagaacgt     2520

attgaagaag cgaaaaacct gaccagcgat agcattaact atattaaaga tgaatttaaa     2580

ctgattgaaa gcattagcga tgcgctgtgc gatctgaaac agcagaacga actggaagac     2640

agccatttta ttagctttga agatattagc gaaaccgatg aaggctttag cattcgtttt     2700

attaacaaag aaaccggcga aagcattttt gtggaaaccg aaaaaaccat ttttagcgaa     2760

tacgcgaacc atatcaccga agaaatcagc aaaatcaaag gcaccatttt tgataccgtg     2820

aacggcaaac tggtgaaaaa agtgaacctg gataccaccc atgaagtgaa caccctgaac     2880

gcggcatttt tcattcagag cctgattgaa tataacagca gcaaagaaag cctgagcaac     2940

ctgagcgtgg cgatgaaagt gcaggtgtat gcgcagctgt ttagcaccgg cctgaacacc     3000

attaccgacg cggcgaaagt ggtggaactg gttagcaccg cgctggatga aaccattgat     3060

ctgctgccga ccctgagcga aggcctgccg attattgcga ccattattga tggcgtgagc     3120

ctgggcgcgg cgattaaaga actgagcgaa accagcgatc cgctgctgcg tcaggaaatt     3180

gaagcgaaaa ttggcattat ggcggtgaac ctgaccaccg cgaccaccgc gattattacc     3240

agcagcctgg gcattgcgag cggctttagc attctgctgg tgccgctggc gggcattagc     3300

gcgggcattc cgagcctggt gaacaacgaa ctggtgctgc gtgataaagc gaccaaagtg     3360

gtggattatt ttaaacatgt gagcctggtg gaaaccgaag gcgtgtttac cctgctggat     3420

gataaaatta tgatgccgca ggatgatctg gtgattagcg aaattgattt taacaacaac     3480

agcattgtgc tgggcaaatg cgaaatttgg cgtatggaag gcggcagcgg ccataccgtg     3540

accgatgata ttgatcattt ctttagcgcg ccgagcatta cctatcgtga accgcatctg     3600

agcatttatg atgtgctgga agtgcagaaa gaagaactgg atctgagcaa agatctgatg     3660

gtgctgccga acgcaccgaa ccgtgtgttt gcatgggaaa ccggttggac cccgggtctg     3720

cgtagcctgg aaaacgatgg caccaaactg ctggatcgta ttcgtgataa ctatgaaggc     3780

gaattttatt ggcgttattt tgcgtttatt gcggatgcgc tgattaccac cctgaaaccg     3840

cgttatgaag ataccaacat tcgtattaac ctggatagca acacccgtag ctttattgtg     3900

ccgattatta ccaccgaata tattcgtgaa aaactgagct atagctttta tggcagcggc     3960

ggcacctatg cgctgagcct gagccagtat aacatgggca ttaacattga actgagcgaa     4020

agcgatgtgt ggattattga tgtggataac gtggtgcgtg atgtgaccat tgaaagcgat     4080

aaaattaaaa aaggcgatct gattgaaggc attctgagca ccctgagcat tgaagaaaac     4140

aaaattattc tgaacagcca tgaaattaac tttagcggcg aagtgaacgg cagcaacggc     4200

tttgtgagcc tgacctttag cattctggaa ggcattaacg cgattattga agtggatctg     4260

ctgagcaaaa gctataaact gctgattagc ggcgaactga aaattctgat gctgaacagc     4320

aaccatattc agcagaaaat tgattatatt ggctttaaca gcgaactgca gaaaaacatt     4380

ccgtatagct ttgtggatag cgaaggcaaa gaaaacggct ttattaacgg cagcaccaaa     4440

gaaggcctgt ttgtgagcga actgccggat gtggtgctga ttagcaaagt gtatatggat     4500

gatagcaaac cgagctttgg ctattatagc aacaacctga aagatgtgaa agtgattacc     4560

aaagataacg tgaacattct gaccggctat tatctgaaag atgatattaa aattagcctg     4620

agcctgaccc tgcaggatga aaaaaccatt aaactgaaca gcgtgcatct ggatgaaagc     4680

ggcgtggcgg aaattctgaa atttatgaac cgtaaaggca acaccaacac cagcgatagc     4740

ctgatgagct ttctggaaag catgaacatc aaaagcatct tcgtgaactt tctgcagagc     4800

aacatcaaat tcatcctgga tgcgaacttc atcatcagcg gcaccaccag cattggccag     4860

ttcgaattta tttgcgatga aaacgataac atccagccgt acttcatcaa attcaacacc     4920

ctggaaacca actataccct gtatgtgggc aaccgtcaga acatgattgt ggaaccgaac     4980

tatgatctgg atgatagcgg cgatattagc agcaccgtga ttaactttag ccagaaatat     5040

ctgtatggca ttgatagctg cgtgaacaaa gtggtgatta gcccgaacat ttataccgat     5100

gaaattaaca ttaccccggt gtatgaaacc aacaacacct atccggaagt gattgtgctg     5160

gatgcgaact atattaacga aaaaattaac gtgaacatta acgatctgag cattcgttat     5220

gtgtggagca acgatggcaa cgattttatt ctgatgagca ccagcgaaga aaacaaagtg     5280

agccaggtga aaattcgttt tgtgaacgtg tttaaagata aaaccctggc gaacaaactg     5340

agctttaact ttagcgataa acaggatgtg cctgtgagcg aaattattct gagctttacc     5400

ccgagctatt atgaagatgg cctgattggc tatgatctgg gcctggttag cctgtataac     5460

gaaaaatttt atattaacaa ctttggcatg atggttagcg gcctgattta tattaacgat     5520

agcctgtatt attttaaacc gccggtgaac aacctgatta ccggctttgt gaccgtgggc     5580

gatgataaat attattttaa cccgattaac ggcggcgcgg cgagcattgg cgaaaccatt     5640

attgatgata aaaactatta ttttaaccag agcggcgtgc tgcagaccgg cgtgtttagc     5700

accgaagatg gctttaaata ttttgcgccg gcgaacaccc tggatgaaaa cctggaaggc     5760

gaagcgatcg atttcaccgg caaactgatc atcgatgaaa acatctacta cttcgatgat     5820

aactaccgtg gcgcggtgga atggaaagaa ctggatggcg aaatgcatta ttttagcccg     5880

gaaaccggca aagcgtttaa aggcctgaac cagattggcg attataaata ttattttaac     5940

agcgatggcg tgatgcagaa aggctttgtg agcattaacg ataacaaaca ttattttgat     6000

gatagcggcg tgatgaaagt gggctatacc gaaattgatg gcaaacattt ttattttgcg     6060

gaaaacggcg aaatgcagat tggcgtgttt aatacggaag acggttttaa atattttgcg     6120

catcataacg aagatctggg caacgaagaa ggcgaagaaa tcagctacag cggcattctg     6180

aactttaaca acaaaattta ttacttcgat gatagcttta ccgcggtggt gggctggaaa     6240

gatctggaag atggcagcaa atattatttt gatgaagata ccgcggaagc gtatattggc     6300

ctgagcctga ttaacgatgg ccagtattat tttaacgatg atggcattat gcaagtgggc     6360

tttgtgacca ttaacgataa agtgttttat tttagcgata gcggcattat tgaaagcggc     6420

gtgcagaaca ttgatgataa ctatttttat attgatgata acggcattgt gcagattggc     6480

gtgtttgata ccagcgatgg ctacaaatac ttcgccccgg cgaacaccgt gaacgataac     6540

atttatggcc aggcggtgga atatagcggc ctggtgcgtg tgggcgaaga tgtgtattat     6600

tttggcgaaa cctataccat tgaaaccggc tggatctacg atatggaaaa cgaaagcgat     6660

aaatactact tcaacccgga aaccaaaaaa gcgtgcaaag gcatcaacct gatcgatgat     6720

atcaaatact acttcgatga aaaaggcatc atgcgtaccg gcctgattag ctttgaaaac     6780

aacaattact acttcaacga aaatggtgaa atgcagttcg gctacatcaa catcgaagat     6840

aaaatgtttt attttggcga agatggcgtg atgcaaatcg gcgtgtttaa caccccggac     6900

ggtttcaaat attttgcgca tcaaaatacc ctggatgaaa actttgaagg cgaaagcatt     6960

aactataccg gctggctgga tctggatgaa aaacgttatt attttaccga tgaatatatt     7020

gcggcgaccg gcagcgtgat tattgatggc gaagaatatt attttgatcc ggataccgcg     7080

cagctggtga ttagcgaata a                                               7101


<210>  11
<211>  7121
<212>  DNA
<213>  Clostridium difficile


<220>
<221>  gene
<222>  (11)..(7111)

<400>  11
cggcagccat atgagcctgg tgaaccgtaa acagctggaa aaaatggcga atgtgcgttt       60

tcgcgttcag gaagatgaat acgttgcgat tctggatgcc ctggaagaat atcataacat      120

gagcgaaaat accgtggttg aaaaatacct gaaactgaaa gatatcaaca gtctgaccga      180

tatctacatc gatacgtaca aaaaatctgg ccgcaacaaa gccctgaaaa aattcaaaga      240

atacctggtg accgaagttc tggaactgaa aaacaataac ctgacgccgg tggagaaaaa      300

cctgcacttt gtttggatcg gcggtcagat caacgatacc gcaatcaatt acatcaacca      360

gtggaaagat gtgaactctg attacaacgt gaacgtgttt tatgatagta atgcgttcct      420

gattaacacc ctgaagaaaa ccatcgttga aagcgccacg aacgataccc tggaatcttt      480

ccgtgaaaac ctgaatgatc cgcgcttcga ttacaacaaa ttctaccgta aacgcatgga      540

aatcatctac gataaacaga aaaacttcat caactactac aaaacccagc gtgaagaaaa      600

cccggatctg atcatcgatg atatcgtgaa aatctacctg agtaacgaat acagcaaaga      660

tatcgatgaa ctgaacagct atatcgaaga atctctgaat aaagtgaccg aaaactctgg      720

caatgatgtt cgcaactttg aagaattcaa aggcggtgaa agttttaaac tgtacgaaca      780

ggaactggtg gaacgttgga atctggcggc cgcaagtgat atcctgcgca ttagcgcgct      840

gaaagaagtg ggcggtgttt atctggatgt tgatatgctg ccgggtattc agccggatct      900

gttcgaaagc atcgaaaaac cgagctctgt gaccgttgat ttttgggaaa tggtgaaact      960

ggaagcaatc atgaaataca aagaatacat cccgggctat acgagcgaac acttcgatat     1020

gctggatgaa gaagtgcaga gtagctttga atctgttctg gcgagtaaaa gcgataaaag     1080

tgaaattttc tctagtctgg gtgatatgga agcgagcccg ctggaagtta aaatcgcctt     1140

caactctaaa ggcatcatca accagggtct gatcagtgtg aaagattctt actgcagtaa     1200

cctgatcgtt aaacagatcg aaaaccgtta caaaatcctg aataactctc tgaacccggc     1260

cattagtgaa gataacgatt ttaataccac gaccaatgca ttcatcgata gcattatggc     1320

cgaagcaaac gcggataatg gccgttttat gatggaactg ggtaaatatc tgcgcgtggg     1380

ctttttcccg gatgttaaaa cgaccattaa cctgagcggt ccggaagcgt acgcggcagc     1440

atatcaggat ctgctgatgt tcaaagaagg cagtatgaat atccacctga ttgaagcgga     1500

tctgcgtaac ttcgaaatca gcaaaaccaa catctctcag agtacggaac aggaaatggc     1560

cagcctgtgg tctttcgatg atgcccgcgc aaaagcgcag ttcgaagaat acaagaaaaa     1620

ctacttcgaa ggcagcctgg gtgaagatga taatctggat ttttctcaga acaccgtggt     1680

tgataaagaa tacctgctgg aaaaaatcag ctctctggcg cgtagtagcg aacgcggtta     1740

catccattat attgtgcagc tgcagggcga taaaatcagc tacgaagcgg cctgcaatct     1800

gtttgcaaaa accccgtatg atagtgttct gttccagaaa aacattgaag atagcgaaat     1860

cgcgtattac tataatccgg gcgatggtga aatccaggaa atcgataaat acaaaatccc     1920

gagtatcatc agcgatcgcc cgaaaattaa actgaccttt atcggccacg gtaaagatga     1980

atttaacacg gatattttcg cgggcctgga tgtggatagc ctgtctaccg aaatcgaaac     2040

ggcaattgat ctggcgaaag aagatatctc tccgaaaagt atcgaaatca atctgctggg     2100

ttgtaacatg tttagttaca gcgtgaatgt tgaagaaacc tatccgggca aactgctgct     2160

gcgtgtgaaa gataaagttt ctgaactgat gccgtctatc agtcaggatt ctattatcgt     2220

gagtgccaat cagtatgaag ttcgcattaa cagcgaaggt cgtcgcgaac tgctggatca     2280

tagcggcgaa tggatcaaca aagaagaatc tatcatcaaa gatatctcta gtaaagaata     2340

catcagcttc aacccgaaag aaaacaaaat catcgtgaaa agtaaaaacc tgccggaact     2400

gagcaccctg ctgcaggaaa tccgtaataa cagcaatagc tctgatattg aactggaaga     2460

aaaagtgatg ctggcagaat gcgaaatcaa cgttatctct aacatcgata cccaggtggt     2520

tgaaggtcgc attgaagaag cgaaaagtct gacgagcgat tctatcaact acatcaaaaa     2580

cgaattcaaa ctgattgaaa gtatcagcga tgcgctgtat gatctgaaac agcagaacga     2640

actggaagaa agccacttta tttctttcga agatatcctg gaaaccgatg aaggtttctc     2700

tatccgtttc atcgataaag aaaccggcga aagtattttt gtggaaacgg aaaaagcaat     2760

cttcagcgaa tacgcgaacc atatcaccga agaaatctct aaaatcaaag gtaccatctt     2820

tgatacggtg aacggcaaac tggtgaaaaa agttaatctg gatgccaccc acgaagttaa     2880

cacgctgaat gcagcgtttt tcatccagag cctgattgaa tacaacagta gcaaagaatc     2940

tctgagtaat ctgagcgtgg caatgaaagt gcaggtttat gcgcagctgt tttctaccgg     3000

cctgaacacg attaccgatg ccgcaaaagt ggttgaactg gttagcaccg ccctggatga     3060

aacgattgat ctgctgccga ccctgagcga aggtctgccg gtgatcgcaa cgattatcga     3120

tggcgtttct ctgggtgcgg ccattaaaga actgagcgaa acctctgatc cgctgctgcg     3180

tcaggaaatt gaagccaaaa ttggcatcat ggcagtgaat ctgaccgcag cgacgaccgc     3240

aattatcacg tctagtctgg gcattgcgag tggttttagc atcctgctgg tgccgctggc     3300

aggtatcagt gcaggtattc cgagcctggt taataacgaa ctgatcctgc gcgataaagc     3360

gaccaaagtg gttgattact tctctcatat cagtctggcg gaaagcgaag gtgcctttac     3420

gtctctggat gataaaatta tgatgccgca ggatgatctg gtgatcagtg aaatcgattt     3480

caacaacaac agcattaccc tgggtaaatg tgaaatctgg cgtatggaag gcggtagcgg     3540

ccatacggtt accgatgata tcgatcactt tttcagcgcc ccgtctatta cctaccgcga     3600

accgcacctg agcatctatg atgtgctgga agttcagaaa gaagaactgg atctgtctaa     3660

agatctgatg gtgctgccga acgcgccgaa tcgtgttttc gcctgggaaa cgggttggac     3720

cccgggtctg cgtagcctgg aaaacgatgg taccaaactg ctggatcgta ttcgcgataa     3780

ttacgaaggc gaattttact ggcgttattt tgcgttcatt gccgatgcac tgatcacgac     3840

cctgaaaccg cgttatgaag ataccaacat tcgcatcaat ctggatagta acacgcgtag     3900

cttcatcgtg ccggttatta cgaccgaata tattcgcgaa aaactgagct actcttttta     3960

tggcagcggc ggtacctacg ccctgagtct gagccagtac aacatgaaca tcaacatcga     4020

actgaacgaa aacgatacct gggtgattga tgttgataac gtggttcgcg atgtgacgat     4080

cgaaagcgat aaaatcaaaa aaggtgatct gatcgaaaac atcctgagta aactgagcat     4140

cgaagataac aaaatcatcc tggataacca tgaaatcaac tttagcggta ccctgaacgg     4200

cggtaatggc tttgtttctc tgacgttcag tatcctggaa ggtattaatg cggtgatcga     4260

agttgatctg ctgtctaaaa gttataaagt gctgattagc ggcgaactga aaaccctgat     4320

ggccaatagc aactctgttc agcagaaaat tgattacatc ggtctgaata gtgaactgca     4380

gaaaaacatc ccgtatagct ttatggatga taaaggcaaa gaaaacggtt tcatcaactg     4440

cagcaccaaa gaaggcctgt ttgtgtctga actgagtgat gtggttctga tctctaaagt     4500

ttacatggat aacagtaaac cgctgtttgg ctattgtagc aatgatctga aagatgtgaa     4560

agttatcacc aaagatgatg tgatcatcct gacgggttac tacctgaaag atgatatcaa     4620

aatcagtctg agcttcacca ttcaggatga aaatacgatc aaactgaacg gtgtgtatct     4680

ggatgaaaac ggcgttgcgg aaatcctgaa attcatgaac aaaaaaggca gcacgaacac     4740

ctctgatagt ctgatgagct tcctggaatc tatgaacatc aaatctatct tcatcaatag     4800

cctgcagtct aacaccaaac tgatcctgga tacgaatttc atcatcagtg gcacgaccag     4860

cattggccag ttcgaattca tctgcgataa agataacaac atccagccgt acttcatcaa     4920

attcaacacg ctggaaacca aatacacgct gtatgtgggt aaccgtcaga atatgattgt     4980

tgaaccgaac tatgatctgg atgatagtgg tgatattagc tctaccgtga tcaattttag     5040

ccagaaatac ctgtatggca tcgattcttg tgtgaacaaa gttatcatca gtccgaacat     5100

ctacaccgat gaaatcaaca tcacgccgat ctacgaagcg aacaatacct atccggaagt     5160

gattgttctg gatacgaact acatctctga aaaaatcaac atcaacatca acgatctgag     5220

tattcgttat gtgtggtcta acgatggcag tgattttatc ctgatgagca ccgatgaaga     5280

aaacaaagtg tctcaggtta aaatccgctt caccaacgtt ttcaagggta acacgatcag     5340

cgataaaatc tctttcaact tcagtgataa acaggatgtg agcatcaaca aagttatctc     5400

tacgttcacc ccgagttact atgtggaagg tctgctgaac tacgatctgg gcctgattag     5460

cctgtacaac gaaaaattct acatcaacaa cttcggcatg atggtgtctg gtctggttta     5520

catcaacgat agcctgtact acttcaaacc gccgattaaa aatctgatca ccggcttcac     5580

gaccatcggt gatgataaat actacttcaa cccggataat ggcggtgccg caagcgtggg     5640

tgaaaccatc atcgatggca aaaactacta cttcagccag aacggcgtgc tgcagaccgg     5700

tgtgtttagc acggaagatg gctttaaata tttcgcgccg gccgataccc tggatgaaaa     5760

tctggaaggt gaagcgatcg atttcaccgg caaactgacg attgatgaaa acgtgtacta     5820

cttcggtgat aactatcgtg cggccattga atggcagacc ctggatgatg aagtttacta     5880

cttctctacg gataccggcc gcgccttcaa aggtctgaac cagatcggcg atgataaatt     5940

ctacttcaac agcgatggta tcatgcagaa aggcttcgtg aacatcaacg ataaaacctt     6000

ctacttcgat gatagcggtg ttatgaaatc tggctatacg gaaatcgatg gcaaatactt     6060

ttatttcgcg gaaaacggtg aaatgcagat tggcgtgttt aataccgccg atggttttaa     6120

atacttcgca catcacgatg aagatctggg caacgaagaa ggtgaagccc tgagctactc     6180

tggcattctg aacttcaaca acaaaatcta ctacttcgat gatagtttca ccgcagtggt     6240

tggttggaaa gatctggaag atggcagcaa atactatttt gatgaagata cggcagaagc     6300

gtatatcggt atctctatca tcaacgatgg caaatactac ttcaacgata gtggtatcat     6360

gcagatcggc ttcgtgacca tcaacaacga agtgttttat ttcagtgata gcggtatcgt     6420

ggaaagcggc atgcagaaca tcgatgacaa ctacttctac atcgatgaaa acggtctggt     6480

gcagattggc gttttcgata cctctgatgg ttacaaatac ttcgccccgg caaatacggt     6540

gaacgataat atctacggcc aggcagttga atatagcggt ctggtgcgtg ttggcgaaga     6600

tgtgtactat tttggtgaaa cgtacaccat tgaaaccggc tggatctatg atatggaaaa     6660

cgaaagcgat aaatactact tcgatccgga aacgaaaaaa gcgtacaaag gcatcaacgt     6720

tatcgatgat atcaaatact acttcgatga aaacggtatt atgcgcaccg gcctgatcac     6780

gttcgaagat aaccattact acttcaacga agatggtatc atgcagtacg gctacctgaa     6840

catcgaagac aaaacgttct acttctctga agacggtatc atgcagattg gcgtgttcaa     6900

taccccggat ggttttaaat atttcgccca ccagaatacg ctggatgaaa acttcgaagg     6960

tgaaagcatc aactacaccg gctggctgga tctggatgaa aaacgctact atttcaccga     7020

tgaatatatc gcagcgacgg gtagtgtgat tatcgatggc gaagaatact attttgatcc     7080

ggataccgca cagctggtta ttagcgaata aggatccggc t                         7121


<210>  12
<211>  2366
<212>  PRT
<213>  Clostridium difficile


<220>
<221>  PEPTIDE
<222>  (1)..(2366)

<400>  12

Met Ser Leu Val Asn Arg Lys Gln Leu Glu Lys Met Ala Asn Val Arg 
1               5                   10                  15      


Phe Arg Val Gln Glu Asp Glu Tyr Val Ala Ile Leu Asp Ala Leu Glu 
            20                  25                  30          


Glu Tyr His Asn Met Ser Glu Asn Thr Val Val Glu Lys Tyr Leu Lys 
        35                  40                  45              


Leu Lys Asp Ile Asn Ser Leu Thr Asp Ile Tyr Ile Asp Thr Tyr Lys 
    50                  55                  60                  


Lys Ser Gly Arg Asn Lys Ala Leu Lys Lys Phe Lys Glu Tyr Leu Val 
65                  70                  75                  80  


Thr Glu Val Leu Glu Leu Lys Asn Asn Asn Leu Thr Pro Val Glu Lys 
                85                  90                  95      


Asn Leu His Phe Val Ala Ile Gly Gly Gln Ile Asn Asp Thr Ala Ile 
            100                 105                 110         


Asn Tyr Ile Asn Gln Trp Lys Asp Val Asn Ser Asp Tyr Asn Val Asn 
        115                 120                 125             


Val Phe Tyr Asp Ser Asn Ala Phe Leu Ile Asn Thr Leu Lys Lys Thr 
    130                 135                 140                 


Ile Val Glu Ser Ala Thr Asn Asp Thr Leu Glu Ser Phe Arg Glu Asn 
145                 150                 155                 160 


Leu Asn Asp Pro Arg Phe Asp Tyr Asn Lys Phe Tyr Arg Lys Arg Met 
                165                 170                 175     


Glu Ile Ile Tyr Asp Lys Gln Lys Asn Phe Ile Asn Tyr Tyr Lys Thr 
            180                 185                 190         


Gln Arg Glu Glu Asn Pro Asp Leu Ile Ile Asp Asp Ile Val Lys Ile 
        195                 200                 205             


Tyr Leu Ser Asn Glu Tyr Ser Lys Asp Ile Asp Glu Leu Asn Ser Tyr 
    210                 215                 220                 


Ile Glu Glu Ser Leu Asn Lys Val Thr Glu Asn Ser Gly Asn Asp Val 
225                 230                 235                 240 


Arg Asn Phe Glu Glu Phe Lys Gly Gly Glu Ser Phe Lys Leu Tyr Glu 
                245                 250                 255     


Gln Glu Leu Val Glu Arg Trp Asn Leu Ala Ala Ala Ser Asp Ile Leu 
            260                 265                 270         


Arg Ile Ser Ala Leu Lys Glu Val Gly Gly Val Tyr Leu Asp Val Asp 
        275                 280                 285             


Met Leu Pro Gly Ile Gln Pro Asp Leu Phe Glu Ser Ile Glu Lys Pro 
    290                 295                 300                 


Ser Ser Val Thr Val Asp Phe Trp Glu Met Val Lys Leu Glu Ala Ile 
305                 310                 315                 320 


Met Lys Tyr Lys Glu Tyr Ile Pro Gly Tyr Thr Ser Glu His Phe Asp 
                325                 330                 335     


Met Leu Asp Glu Glu Val Gln Ser Ser Phe Glu Ser Val Leu Ala Ser 
            340                 345                 350         


Lys Ser Asp Lys Ser Glu Ile Phe Ser Ser Leu Gly Asp Met Glu Ala 
        355                 360                 365             


Ser Pro Leu Glu Val Lys Ile Ala Phe Asn Ser Lys Gly Ile Ile Asn 
    370                 375                 380                 


Gln Gly Leu Ile Ser Val Lys Asp Ser Tyr Cys Ser Asn Leu Ile Val 
385                 390                 395                 400 


Lys Gln Ile Glu Asn Arg Tyr Lys Ile Leu Asn Asn Ser Leu Asn Pro 
                405                 410                 415     


Ala Ile Ser Glu Asp Asn Asp Phe Asn Thr Thr Thr Asn Ala Phe Ile 
            420                 425                 430         


Asp Ser Ile Met Ala Glu Ala Asn Ala Asp Asn Gly Arg Phe Met Met 
        435                 440                 445             


Glu Leu Gly Lys Tyr Leu Arg Val Gly Phe Phe Pro Asp Val Lys Thr 
    450                 455                 460                 


Thr Ile Asn Leu Ser Gly Pro Glu Ala Tyr Ala Ala Ala Tyr Gln Asp 
465                 470                 475                 480 


Leu Leu Met Phe Lys Glu Gly Ser Met Asn Ile His Leu Ile Glu Ala 
                485                 490                 495     


Asp Leu Arg Asn Phe Glu Ile Ser Lys Thr Asn Ile Ser Gln Ser Thr 
            500                 505                 510         


Glu Gln Glu Met Ala Ser Leu Trp Ser Phe Asp Asp Ala Arg Ala Lys 
        515                 520                 525             


Ala Gln Phe Glu Glu Tyr Lys Lys Asn Tyr Phe Glu Gly Ser Leu Gly 
    530                 535                 540                 


Glu Asp Asp Asn Leu Asp Phe Ser Gln Asn Thr Val Val Asp Lys Glu 
545                 550                 555                 560 


Tyr Leu Leu Glu Lys Ile Ser Ser Leu Ala Arg Ser Ser Glu Arg Gly 
                565                 570                 575     


Tyr Ile His Tyr Ile Val Gln Leu Gln Gly Asp Lys Ile Ser Tyr Glu 
            580                 585                 590         


Ala Ala Cys Asn Leu Phe Ala Lys Thr Pro Tyr Asp Ser Val Leu Phe 
        595                 600                 605             


Gln Lys Asn Ile Glu Asp Ser Glu Ile Ala Tyr Tyr Tyr Asn Pro Gly 
    610                 615                 620                 


Asp Gly Glu Ile Gln Glu Ile Asp Lys Tyr Lys Ile Pro Ser Ile Ile 
625                 630                 635                 640 


Ser Asp Arg Pro Lys Ile Lys Leu Thr Phe Ile Gly His Gly Lys Asp 
                645                 650                 655     


Glu Phe Asn Thr Asp Ile Phe Ala Gly Leu Asp Val Asp Ser Leu Ser 
            660                 665                 670         


Thr Glu Ile Glu Thr Ala Ile Asp Leu Ala Lys Glu Asp Ile Ser Pro 
        675                 680                 685             


Lys Ser Ile Glu Ile Asn Leu Leu Gly Cys Asn Met Phe Ser Tyr Ser 
    690                 695                 700                 


Val Asn Val Glu Glu Thr Tyr Pro Gly Lys Leu Leu Leu Arg Val Lys 
705                 710                 715                 720 


Asp Lys Val Ser Glu Leu Met Pro Ser Ile Ser Gln Asp Ser Ile Ile 
                725                 730                 735     


Val Ser Ala Asn Gln Tyr Glu Val Arg Ile Asn Ser Glu Gly Arg Arg 
            740                 745                 750         


Glu Leu Leu Asp His Ser Gly Glu Trp Ile Asn Lys Glu Glu Ser Ile 
        755                 760                 765             


Ile Lys Asp Ile Ser Ser Lys Glu Tyr Ile Ser Phe Asn Pro Lys Glu 
    770                 775                 780                 


Asn Lys Ile Ile Val Lys Ser Lys Asn Leu Pro Glu Leu Ser Thr Leu 
785                 790                 795                 800 


Leu Gln Glu Ile Arg Asn Asn Ser Asn Ser Ser Asp Ile Glu Leu Glu 
                805                 810                 815     


Glu Lys Val Met Leu Ala Glu Cys Glu Ile Asn Val Ile Ser Asn Ile 
            820                 825                 830         


Asp Thr Gln Val Val Glu Gly Arg Ile Glu Glu Ala Lys Ser Leu Thr 
        835                 840                 845             


Ser Asp Ser Ile Asn Tyr Ile Lys Asn Glu Phe Lys Leu Ile Glu Ser 
    850                 855                 860                 


Ile Ser Asp Ala Leu Tyr Asp Leu Lys Gln Gln Asn Glu Leu Glu Glu 
865                 870                 875                 880 


Ser His Phe Ile Ser Phe Glu Asp Ile Leu Glu Thr Asp Glu Gly Phe 
                885                 890                 895     


Ser Ile Arg Phe Ile Asp Lys Glu Thr Gly Glu Ser Ile Phe Val Glu 
            900                 905                 910         


Thr Glu Lys Ala Ile Phe Ser Glu Tyr Ala Asn His Ile Thr Glu Glu 
        915                 920                 925             


Ile Ser Lys Ile Lys Gly Thr Ile Phe Asp Thr Val Asn Gly Lys Leu 
    930                 935                 940                 


Val Lys Lys Val Asn Leu Asp Ala Thr His Glu Val Asn Thr Leu Asn 
945                 950                 955                 960 


Ala Ala Phe Phe Ile Gln Ser Leu Ile Glu Tyr Asn Ser Ser Lys Glu 
                965                 970                 975     


Ser Leu Ser Asn Leu Ser Val Ala Met Lys Val Gln Val Tyr Ala Gln 
            980                 985                 990         


Leu Phe Ser Thr Gly Leu Asn Thr  Ile Thr Asp Ala Ala  Lys Val Val 
        995                 1000                 1005             


Glu Leu  Val Ser Thr Ala Leu  Asp Glu Thr Ile Asp  Leu Leu Pro 
    1010                 1015                 1020             


Thr Leu  Ser Glu Gly Leu Pro  Val Ile Ala Thr Ile  Ile Asp Gly 
    1025                 1030                 1035             


Val Ser  Leu Gly Ala Ala Ile  Lys Glu Leu Ser Glu  Thr Ser Asp 
    1040                 1045                 1050             


Pro Leu  Leu Arg Gln Glu Ile  Glu Ala Lys Ile Gly  Ile Met Ala 
    1055                 1060                 1065             


Val Asn  Leu Thr Ala Ala Thr  Thr Ala Ile Ile Thr  Ser Ser Leu 
    1070                 1075                 1080             


Gly Ile  Ala Ser Gly Phe Ser  Ile Leu Leu Val Pro  Leu Ala Gly 
    1085                 1090                 1095             


Ile Ser  Ala Gly Ile Pro Ser  Leu Val Asn Asn Glu  Leu Ile Leu 
    1100                 1105                 1110             


Arg Asp  Lys Ala Thr Lys Val  Val Asp Tyr Phe Ser  His Ile Ser 
    1115                 1120                 1125             


Leu Ala  Glu Ser Glu Gly Ala  Phe Thr Ser Leu Asp  Asp Lys Ile 
    1130                 1135                 1140             


Met Met  Pro Gln Asp Asp Leu  Val Ile Ser Glu Ile  Asp Phe Asn 
    1145                 1150                 1155             


Asn Asn  Ser Ile Thr Leu Gly  Lys Cys Glu Ile Trp  Arg Met Glu 
    1160                 1165                 1170             


Gly Gly  Ser Gly His Thr Val  Thr Asp Asp Ile Asp  His Phe Phe 
    1175                 1180                 1185             


Ser Ala  Pro Ser Ile Thr Tyr  Arg Glu Pro His Leu  Ser Ile Tyr 
    1190                 1195                 1200             


Asp Val  Leu Glu Val Gln Lys  Glu Glu Leu Asp Leu  Ser Lys Asp 
    1205                 1210                 1215             


Leu Met  Val Leu Pro Asn Ala  Pro Asn Arg Val Phe  Ala Trp Glu 
    1220                 1225                 1230             


Thr Gly  Trp Thr Pro Gly Leu  Arg Ser Leu Glu Asn  Asp Gly Thr 
    1235                 1240                 1245             


Lys Leu  Leu Asp Arg Ile Arg  Asp Asn Tyr Glu Gly  Glu Phe Tyr 
    1250                 1255                 1260             


Trp Arg  Tyr Phe Ala Phe Ile  Ala Asp Ala Leu Ile  Thr Thr Leu 
    1265                 1270                 1275             


Lys Pro  Arg Tyr Glu Asp Thr  Asn Ile Arg Ile Asn  Leu Asp Ser 
    1280                 1285                 1290             


Asn Thr  Arg Ser Phe Ile Val  Pro Val Ile Thr Thr  Glu Tyr Ile 
    1295                 1300                 1305             


Arg Glu  Lys Leu Ser Tyr Ser  Phe Tyr Gly Ser Gly  Gly Thr Tyr 
    1310                 1315                 1320             


Ala Leu  Ser Leu Ser Gln Tyr  Asn Met Asn Ile Asn  Ile Glu Leu 
    1325                 1330                 1335             


Asn Glu  Asn Asp Thr Trp Val  Ile Asp Val Asp Asn  Val Val Arg 
    1340                 1345                 1350             


Asp Val  Thr Ile Glu Ser Asp  Lys Ile Lys Lys Gly  Asp Leu Ile 
    1355                 1360                 1365             


Glu Asn  Ile Leu Ser Lys Leu  Ser Ile Glu Asp Asn  Lys Ile Ile 
    1370                 1375                 1380             


Leu Asp  Asn His Glu Ile Asn  Phe Ser Gly Thr Leu  Asn Gly Gly 
    1385                 1390                 1395             


Asn Gly  Phe Val Ser Leu Thr  Phe Ser Ile Leu Glu  Gly Ile Asn 
    1400                 1405                 1410             


Ala Val  Ile Glu Val Asp Leu  Leu Ser Lys Ser Tyr  Lys Val Leu 
    1415                 1420                 1425             


Ile Ser  Gly Glu Leu Lys Thr  Leu Met Ala Asn Ser  Asn Ser Val 
    1430                 1435                 1440             


Gln Gln  Lys Ile Asp Tyr Ile  Gly Leu Asn Ser Glu  Leu Gln Lys 
    1445                 1450                 1455             


Asn Ile  Pro Tyr Ser Phe Met  Asp Asp Lys Gly Lys  Glu Asn Gly 
    1460                 1465                 1470             


Phe Ile  Asn Cys Ser Thr Lys  Glu Gly Leu Phe Val  Ser Glu Leu 
    1475                 1480                 1485             


Ser Asp  Val Val Leu Ile Ser  Lys Val Tyr Met Asp  Asn Ser Lys 
    1490                 1495                 1500             


Pro Leu  Phe Gly Tyr Cys Ser  Asn Asp Leu Lys Asp  Val Lys Val 
    1505                 1510                 1515             


Ile Thr  Lys Asp Asp Val Ile  Ile Leu Thr Gly Tyr  Tyr Leu Lys 
    1520                 1525                 1530             


Asp Asp  Ile Lys Ile Ser Leu  Ser Phe Thr Ile Gln  Asp Glu Asn 
    1535                 1540                 1545             


Thr Ile  Lys Leu Asn Gly Val  Tyr Leu Asp Glu Asn  Gly Val Ala 
    1550                 1555                 1560             


Glu Ile  Leu Lys Phe Met Asn  Lys Lys Gly Ser Thr  Asn Thr Ser 
    1565                 1570                 1575             


Asp Ser  Leu Met Ser Phe Leu  Glu Ser Met Asn Ile  Lys Ser Ile 
    1580                 1585                 1590             


Phe Ile  Asn Ser Leu Gln Ser  Asn Thr Lys Leu Ile  Leu Asp Thr 
    1595                 1600                 1605             


Asn Phe  Ile Ile Ser Gly Thr  Thr Ser Ile Gly Gln  Phe Glu Phe 
    1610                 1615                 1620             


Ile Cys  Asp Lys Asp Asn Asn  Ile Gln Pro Tyr Phe  Ile Lys Phe 
    1625                 1630                 1635             


Asn Thr  Leu Glu Thr Lys Tyr  Thr Leu Tyr Val Gly  Asn Arg Gln 
    1640                 1645                 1650             


Asn Met  Ile Val Glu Pro Asn  Tyr Asp Leu Asp Asp  Ser Gly Asp 
    1655                 1660                 1665             


Ile Ser  Ser Thr Val Ile Asn  Phe Ser Gln Lys Tyr  Leu Tyr Gly 
    1670                 1675                 1680             


Ile Asp  Ser Cys Val Asn Lys  Val Ile Ile Ser Pro  Asn Ile Tyr 
    1685                 1690                 1695             


Thr Asp  Glu Ile Asn Ile Thr  Pro Ile Tyr Glu Ala  Asn Asn Thr 
    1700                 1705                 1710             


Tyr Pro  Glu Val Ile Val Leu  Asp Thr Asn Tyr Ile  Ser Glu Lys 
    1715                 1720                 1725             


Ile Asn  Ile Asn Ile Asn Asp  Leu Ser Ile Arg Tyr  Val Trp Ser 
    1730                 1735                 1740             


Asn Asp  Gly Ser Asp Phe Ile  Leu Met Ser Thr Asp  Glu Glu Asn 
    1745                 1750                 1755             


Lys Val  Ser Gln Val Lys Ile  Arg Phe Thr Asn Val  Phe Lys Gly 
    1760                 1765                 1770             


Asn Thr  Ile Ser Asp Lys Ile  Ser Phe Asn Phe Ser  Asp Lys Gln 
    1775                 1780                 1785             


Asp Val  Ser Ile Asn Lys Val  Ile Ser Thr Phe Thr  Pro Ser Tyr 
    1790                 1795                 1800             


Tyr Val  Glu Gly Leu Leu Asn  Tyr Asp Leu Gly Leu  Ile Ser Leu 
    1805                 1810                 1815             


Tyr Asn  Glu Lys Phe Tyr Ile  Asn Asn Phe Gly Met  Met Val Ser 
    1820                 1825                 1830             


Gly Leu  Val Tyr Ile Asn Asp  Ser Leu Tyr Tyr Phe  Lys Pro Pro 
    1835                 1840                 1845             


Ile Lys  Asn Leu Ile Thr Gly  Phe Thr Thr Ile Gly  Asp Asp Lys 
    1850                 1855                 1860             


Tyr Tyr  Phe Asn Pro Asp Asn  Gly Gly Ala Ala Ser  Val Gly Glu 
    1865                 1870                 1875             


Thr Ile  Ile Asp Gly Lys Asn  Tyr Tyr Phe Ser Gln  Asn Gly Val 
    1880                 1885                 1890             


Leu Gln  Thr Gly Val Phe Ser  Thr Glu Asp Gly Phe  Lys Tyr Phe 
    1895                 1900                 1905             


Ala Pro  Ala Asp Thr Leu Asp  Glu Asn Leu Glu Gly  Glu Ala Ile 
    1910                 1915                 1920             


Asp Phe  Thr Gly Lys Leu Thr  Ile Asp Glu Asn Val  Tyr Tyr Phe 
    1925                 1930                 1935             


Gly Asp  Asn Tyr Arg Ala Ala  Ile Glu Trp Gln Thr  Leu Asp Asp 
    1940                 1945                 1950             


Glu Val  Tyr Tyr Phe Ser Thr  Asp Thr Gly Arg Ala  Phe Lys Gly 
    1955                 1960                 1965             


Leu Asn  Gln Ile Gly Asp Asp  Lys Phe Tyr Phe Asn  Ser Asp Gly 
    1970                 1975                 1980             


Ile Met  Gln Lys Gly Phe Val  Asn Ile Asn Asp Lys  Thr Phe Tyr 
    1985                 1990                 1995             


Phe Asp  Asp Ser Gly Val Met  Lys Ser Gly Tyr Thr  Glu Ile Asp 
    2000                 2005                 2010             


Gly Lys  Tyr Phe Tyr Phe Ala  Glu Asn Gly Glu Met  Gln Ile Gly 
    2015                 2020                 2025             


Val Phe  Asn Thr Ala Asp Gly  Phe Lys Tyr Phe Ala  His His Asp 
    2030                 2035                 2040             


Glu Asp  Leu Gly Asn Glu Glu  Gly Glu Ala Leu Ser  Tyr Ser Gly 
    2045                 2050                 2055             


Ile Leu  Asn Phe Asn Asn Lys  Ile Tyr Tyr Phe Asp  Asp Ser Phe 
    2060                 2065                 2070             


Thr Ala  Val Val Gly Trp Lys  Asp Leu Glu Asp Gly  Ser Lys Tyr 
    2075                 2080                 2085             


Tyr Phe  Asp Glu Asp Thr Ala  Glu Ala Tyr Ile Gly  Ile Ser Ile 
    2090                 2095                 2100             


Ile Asn  Asp Gly Lys Tyr Tyr  Phe Asn Asp Ser Gly  Ile Met Gln 
    2105                 2110                 2115             


Ile Gly  Phe Val Thr Ile Asn  Asn Glu Val Phe Tyr  Phe Ser Asp 
    2120                 2125                 2130             


Ser Gly  Ile Val Glu Ser Gly  Met Gln Asn Ile Asp  Asp Asn Tyr 
    2135                 2140                 2145             


Phe Tyr  Ile Asp Glu Asn Gly  Leu Val Gln Ile Gly  Val Phe Asp 
    2150                 2155                 2160             


Thr Ser  Asp Gly Tyr Lys Tyr  Phe Ala Pro Ala Asn  Thr Val Asn 
    2165                 2170                 2175             


Asp Asn  Ile Tyr Gly Gln Ala  Val Glu Tyr Ser Gly  Leu Val Arg 
    2180                 2185                 2190             


Val Gly  Glu Asp Val Tyr Tyr  Phe Gly Glu Thr Tyr  Thr Ile Glu 
    2195                 2200                 2205             


Thr Gly  Trp Ile Tyr Asp Met  Glu Asn Glu Ser Asp  Lys Tyr Tyr 
    2210                 2215                 2220             


Phe Asp  Pro Glu Thr Lys Lys  Ala Tyr Lys Gly Ile  Asn Val Ile 
    2225                 2230                 2235             


Asp Asp  Ile Lys Tyr Tyr Phe  Asp Glu Asn Gly Ile  Met Arg Thr 
    2240                 2245                 2250             


Gly Leu  Ile Thr Phe Glu Asp  Asn His Tyr Tyr Phe  Asn Glu Asp 
    2255                 2260                 2265             


Gly Ile  Met Gln Tyr Gly Tyr  Leu Asn Ile Glu Asp  Lys Thr Phe 
    2270                 2275                 2280             


Tyr Phe  Ser Glu Asp Gly Ile  Met Gln Ile Gly Val  Phe Asn Thr 
    2285                 2290                 2295             


Pro Asp  Gly Phe Lys Tyr Phe  Ala His Gln Asn Thr  Leu Asp Glu 
    2300                 2305                 2310             


Asn Phe  Glu Gly Glu Ser Ile  Asn Tyr Thr Gly Trp  Leu Asp Leu 
    2315                 2320                 2325             


Asp Glu  Lys Arg Tyr Tyr Phe  Thr Asp Glu Tyr Ile  Ala Ala Thr 
    2330                 2335                 2340             


Gly Ser  Val Ile Ile Asp Gly  Glu Glu Tyr Tyr Phe  Asp Pro Asp 
    2345                 2350                 2355             


Thr Ala  Gln Leu Val Ile Ser  Glu 
    2360                 2365     


<210>  13
<211>  2366
<212>  PRT
<213>  Clostridium difficile


<220>
<221>  PEPTIDE
<222>  (1)..(2366)

<400>  13

Met Ser Leu Val Asn Arg Lys Gln Leu Glu Lys Met Ala Asn Val Arg 
1               5                   10                  15      


Phe Arg Val Gln Glu Asp Glu Tyr Val Ala Ile Leu Asp Ala Leu Glu 
            20                  25                  30          


Glu Tyr His Asn Met Ser Glu Asn Thr Val Val Glu Lys Tyr Leu Lys 
        35                  40                  45              


Leu Lys Asp Ile Asn Ser Leu Thr Asp Ile Tyr Ile Asp Thr Tyr Lys 
    50                  55                  60                  


Lys Ser Gly Arg Asn Lys Ala Leu Lys Lys Phe Lys Glu Tyr Leu Val 
65                  70                  75                  80  


Thr Glu Val Leu Glu Leu Lys Asn Asn Asn Leu Thr Pro Val Glu Lys 
                85                  90                  95      


Asn Leu His Phe Val Trp Ile Gly Gly Gln Ile Asn Asp Thr Ala Ile 
            100                 105                 110         


Asn Tyr Ile Asn Gln Trp Lys Asp Val Asn Ser Asp Tyr Asn Val Asn 
        115                 120                 125             


Val Phe Tyr Asp Ser Asn Ala Phe Leu Ile Asn Thr Leu Lys Lys Thr 
    130                 135                 140                 


Ile Val Glu Ser Ala Thr Asn Asp Thr Leu Glu Ser Phe Arg Glu Asn 
145                 150                 155                 160 


Leu Asn Asp Pro Arg Phe Asp Tyr Asn Lys Phe Tyr Arg Lys Arg Met 
                165                 170                 175     


Glu Ile Ile Tyr Asp Lys Gln Lys Asn Phe Ile Asn Tyr Tyr Lys Thr 
            180                 185                 190         


Gln Arg Glu Glu Asn Pro Asp Leu Ile Ile Asp Asp Ile Val Lys Ile 
        195                 200                 205             


Tyr Leu Ser Asn Glu Tyr Ser Lys Asp Ile Asp Glu Leu Asn Ser Tyr 
    210                 215                 220                 


Ile Glu Glu Ser Leu Asn Lys Val Thr Glu Asn Ser Gly Asn Asp Val 
225                 230                 235                 240 


Arg Asn Phe Glu Glu Phe Lys Gly Gly Glu Ser Phe Lys Leu Tyr Glu 
                245                 250                 255     


Gln Glu Leu Val Glu Arg Trp Asn Leu Ala Ala Ala Ser Asp Ile Leu 
            260                 265                 270         


Arg Ile Ser Ala Leu Lys Glu Val Gly Gly Val Tyr Leu Gly Val Gly 
        275                 280                 285             


Met Leu Pro Gly Ile Gln Pro Asp Leu Phe Glu Ser Ile Glu Lys Pro 
    290                 295                 300                 


Ser Ser Val Thr Val Asp Phe Trp Glu Met Val Lys Leu Glu Ala Ile 
305                 310                 315                 320 


Met Lys Tyr Lys Glu Tyr Ile Pro Gly Tyr Thr Ser Glu His Phe Asp 
                325                 330                 335     


Met Leu Asp Glu Glu Val Gln Ser Ser Phe Glu Ser Val Leu Ala Ser 
            340                 345                 350         


Lys Ser Asp Lys Ser Glu Ile Phe Ser Ser Leu Gly Asp Met Glu Ala 
        355                 360                 365             


Ser Pro Leu Glu Val Lys Ile Ala Phe Asn Ser Lys Gly Ile Ile Asn 
    370                 375                 380                 


Gln Gly Leu Ile Ser Val Lys Asp Ser Tyr Cys Ser Asn Leu Ile Val 
385                 390                 395                 400 


Lys Gln Ile Glu Asn Arg Tyr Lys Ile Leu Asn Asn Ser Leu Asn Pro 
                405                 410                 415     


Ala Ile Ser Glu Asp Asn Asp Phe Asn Thr Thr Thr Asn Ala Phe Ile 
            420                 425                 430         


Asp Ser Ile Met Ala Glu Ala Asn Ala Asp Asn Gly Arg Phe Met Met 
        435                 440                 445             


Glu Leu Gly Lys Tyr Leu Arg Val Gly Phe Phe Pro Asp Val Lys Thr 
    450                 455                 460                 


Thr Ile Asn Leu Ser Gly Pro Glu Ala Tyr Ala Ala Ala Tyr Gln Asp 
465                 470                 475                 480 


Leu Leu Met Phe Lys Glu Gly Ser Met Asn Ile His Leu Ile Glu Ala 
                485                 490                 495     


Asp Leu Arg Asn Phe Glu Ile Ser Lys Thr Asn Ile Ser Gln Ser Thr 
            500                 505                 510         


Glu Gln Glu Met Ala Ser Leu Trp Ser Phe Asp Asp Ala Arg Ala Lys 
        515                 520                 525             


Ala Gln Phe Glu Glu Tyr Lys Lys Asn Tyr Phe Glu Gly Ser Leu Gly 
    530                 535                 540                 


Glu Asp Asp Asn Leu Asp Phe Ser Gln Asn Thr Val Val Asp Lys Glu 
545                 550                 555                 560 


Tyr Leu Leu Glu Lys Ile Ser Ser Leu Ala Arg Ser Ser Glu Arg Gly 
                565                 570                 575     


Tyr Ile His Tyr Ile Val Gln Leu Gln Gly Asp Lys Ile Ser Tyr Glu 
            580                 585                 590         


Ala Ala Cys Asn Leu Phe Ala Lys Thr Pro Tyr Asp Ser Val Leu Phe 
        595                 600                 605             


Gln Lys Asn Ile Glu Asp Ser Glu Ile Ala Tyr Tyr Tyr Asn Pro Gly 
    610                 615                 620                 


Asp Gly Glu Ile Gln Glu Ile Asp Lys Tyr Lys Ile Pro Ser Ile Ile 
625                 630                 635                 640 


Ser Asp Arg Pro Lys Ile Lys Leu Thr Phe Ile Gly His Gly Lys Asp 
                645                 650                 655     


Glu Phe Asn Thr Asp Ile Phe Ala Gly Leu Asp Val Asp Ser Leu Ser 
            660                 665                 670         


Thr Glu Ile Glu Thr Ala Ile Asp Leu Ala Lys Glu Asp Ile Ser Pro 
        675                 680                 685             


Lys Ser Ile Glu Ile Asn Leu Leu Gly Cys Asn Met Phe Ser Tyr Ser 
    690                 695                 700                 


Val Asn Val Glu Glu Thr Tyr Pro Gly Lys Leu Leu Leu Arg Val Lys 
705                 710                 715                 720 


Asp Lys Val Ser Glu Leu Met Pro Ser Ile Ser Gln Asp Ser Ile Ile 
                725                 730                 735     


Val Ser Ala Asn Gln Tyr Glu Val Arg Ile Asn Ser Glu Gly Arg Arg 
            740                 745                 750         


Glu Leu Leu Asp His Ser Gly Glu Trp Ile Asn Lys Glu Glu Ser Ile 
        755                 760                 765             


Ile Lys Asp Ile Ser Ser Lys Glu Tyr Ile Ser Phe Asn Pro Lys Glu 
    770                 775                 780                 


Asn Lys Ile Ile Val Lys Ser Lys Asn Leu Pro Glu Leu Ser Thr Leu 
785                 790                 795                 800 


Leu Gln Glu Ile Arg Asn Asn Ser Asn Ser Ser Asp Ile Glu Leu Glu 
                805                 810                 815     


Glu Lys Val Met Leu Ala Glu Cys Glu Ile Asn Val Ile Ser Asn Ile 
            820                 825                 830         


Asp Thr Gln Val Val Glu Gly Arg Ile Glu Glu Ala Lys Ser Leu Thr 
        835                 840                 845             


Ser Asp Ser Ile Asn Tyr Ile Lys Asn Glu Phe Lys Leu Ile Glu Ser 
    850                 855                 860                 


Ile Ser Asp Ala Leu Tyr Asp Leu Lys Gln Gln Asn Glu Leu Glu Glu 
865                 870                 875                 880 


Ser His Phe Ile Ser Phe Glu Asp Ile Leu Glu Thr Asp Glu Gly Phe 
                885                 890                 895     


Ser Ile Arg Phe Ile Asp Lys Glu Thr Gly Glu Ser Ile Phe Val Glu 
            900                 905                 910         


Thr Glu Lys Ala Ile Phe Ser Glu Tyr Ala Asn His Ile Thr Glu Glu 
        915                 920                 925             


Ile Ser Lys Ile Lys Gly Thr Ile Phe Asp Thr Val Asn Gly Lys Leu 
    930                 935                 940                 


Val Lys Lys Val Asn Leu Asp Ala Thr His Glu Val Asn Thr Leu Asn 
945                 950                 955                 960 


Ala Ala Phe Phe Ile Gln Ser Leu Ile Glu Tyr Asn Ser Ser Lys Glu 
                965                 970                 975     


Ser Leu Ser Asn Leu Ser Val Ala Met Lys Val Gln Val Tyr Ala Gln 
            980                 985                 990         


Leu Phe Ser Thr Gly Leu Asn Thr  Ile Thr Asp Ala Ala  Lys Val Val 
        995                 1000                 1005             


Glu Leu  Val Ser Thr Ala Leu  Asp Glu Thr Ile Asp  Leu Leu Pro 
    1010                 1015                 1020             


Thr Leu  Ser Glu Gly Leu Pro  Val Ile Ala Thr Ile  Ile Asp Gly 
    1025                 1030                 1035             


Val Ser  Leu Gly Ala Ala Ile  Lys Glu Leu Ser Glu  Thr Ser Asp 
    1040                 1045                 1050             


Pro Leu  Leu Arg Gln Glu Ile  Glu Ala Lys Ile Gly  Ile Met Ala 
    1055                 1060                 1065             


Val Asn  Leu Thr Ala Ala Thr  Thr Ala Ile Ile Thr  Ser Ser Leu 
    1070                 1075                 1080             


Gly Ile  Ala Ser Gly Phe Ser  Ile Leu Leu Val Pro  Leu Ala Gly 
    1085                 1090                 1095             


Ile Ser  Ala Gly Ile Pro Ser  Leu Val Asn Asn Glu  Leu Ile Leu 
    1100                 1105                 1110             


Arg Asp  Lys Ala Thr Lys Val  Val Asp Tyr Phe Ser  His Ile Ser 
    1115                 1120                 1125             


Leu Ala  Glu Ser Glu Gly Ala  Phe Thr Ser Leu Asp  Asp Lys Ile 
    1130                 1135                 1140             


Met Met  Pro Gln Asp Asp Leu  Val Ile Ser Glu Ile  Asp Phe Asn 
    1145                 1150                 1155             


Asn Asn  Ser Ile Thr Leu Gly  Lys Cys Glu Ile Trp  Arg Met Glu 
    1160                 1165                 1170             


Gly Gly  Ser Gly His Thr Val  Thr Asp Asp Ile Asp  His Phe Phe 
    1175                 1180                 1185             


Ser Ala  Pro Ser Ile Thr Tyr  Arg Glu Pro His Leu  Ser Ile Tyr 
    1190                 1195                 1200             


Asp Val  Leu Glu Val Gln Lys  Glu Glu Leu Asp Leu  Ser Lys Asp 
    1205                 1210                 1215             


Leu Met  Val Leu Pro Asn Ala  Pro Asn Arg Val Phe  Ala Trp Glu 
    1220                 1225                 1230             


Thr Gly  Trp Thr Pro Gly Leu  Arg Ser Leu Glu Asn  Asp Gly Thr 
    1235                 1240                 1245             


Lys Leu  Leu Asp Arg Ile Arg  Asp Asn Tyr Glu Gly  Glu Phe Tyr 
    1250                 1255                 1260             


Trp Arg  Tyr Phe Ala Phe Ile  Ala Asp Ala Leu Ile  Thr Thr Leu 
    1265                 1270                 1275             


Lys Pro  Arg Tyr Glu Asp Thr  Asn Ile Arg Ile Asn  Leu Asp Ser 
    1280                 1285                 1290             


Asn Thr  Arg Ser Phe Ile Val  Pro Val Ile Thr Thr  Glu Tyr Ile 
    1295                 1300                 1305             


Arg Glu  Lys Leu Ser Tyr Ser  Phe Tyr Gly Ser Gly  Gly Thr Tyr 
    1310                 1315                 1320             


Ala Leu  Ser Leu Ser Gln Tyr  Asn Met Asn Ile Asn  Ile Glu Leu 
    1325                 1330                 1335             


Asn Glu  Asn Asp Thr Trp Val  Ile Asp Val Asp Asn  Val Val Arg 
    1340                 1345                 1350             


Asp Val  Thr Ile Glu Ser Asp  Lys Ile Lys Lys Gly  Asp Leu Ile 
    1355                 1360                 1365             


Glu Asn  Ile Leu Ser Lys Leu  Ser Ile Glu Asp Asn  Lys Ile Ile 
    1370                 1375                 1380             


Leu Asp  Asn His Glu Ile Asn  Phe Ser Gly Thr Leu  Asn Gly Gly 
    1385                 1390                 1395             


Asn Gly  Phe Val Ser Leu Thr  Phe Ser Ile Leu Glu  Gly Ile Asn 
    1400                 1405                 1410             


Ala Val  Ile Glu Val Asp Leu  Leu Ser Lys Ser Tyr  Lys Val Leu 
    1415                 1420                 1425             


Ile Ser  Gly Glu Leu Lys Thr  Leu Met Ala Asn Ser  Asn Ser Val 
    1430                 1435                 1440             


Gln Gln  Lys Ile Asp Tyr Ile  Gly Leu Asn Ser Glu  Leu Gln Lys 
    1445                 1450                 1455             


Asn Ile  Pro Tyr Ser Phe Met  Asp Asp Lys Gly Lys  Glu Asn Gly 
    1460                 1465                 1470             


Phe Ile  Asn Cys Ser Thr Lys  Glu Gly Leu Phe Val  Ser Glu Leu 
    1475                 1480                 1485             


Ser Asp  Val Val Leu Ile Ser  Lys Val Tyr Met Asp  Asn Ser Lys 
    1490                 1495                 1500             


Pro Leu  Phe Gly Tyr Cys Ser  Asn Asp Leu Lys Asp  Val Lys Val 
    1505                 1510                 1515             


Ile Thr  Lys Asp Asp Val Ile  Ile Leu Thr Gly Tyr  Tyr Leu Lys 
    1520                 1525                 1530             


Asp Asp  Ile Lys Ile Ser Leu  Ser Phe Thr Ile Gln  Asp Glu Asn 
    1535                 1540                 1545             


Thr Ile  Lys Leu Asn Gly Val  Tyr Leu Asp Glu Asn  Gly Val Ala 
    1550                 1555                 1560             


Glu Ile  Leu Lys Phe Met Asn  Lys Lys Gly Ser Thr  Asn Thr Ser 
    1565                 1570                 1575             


Asp Ser  Leu Met Ser Phe Leu  Glu Ser Met Asn Ile  Lys Ser Ile 
    1580                 1585                 1590             


Phe Ile  Asn Ser Leu Gln Ser  Asn Thr Lys Leu Ile  Leu Asp Thr 
    1595                 1600                 1605             


Asn Phe  Ile Ile Ser Gly Thr  Thr Ser Ile Gly Gln  Phe Glu Phe 
    1610                 1615                 1620             


Ile Cys  Asp Lys Asp Asn Asn  Ile Gln Pro Tyr Phe  Ile Lys Phe 
    1625                 1630                 1635             


Asn Thr  Leu Glu Thr Lys Tyr  Thr Leu Tyr Val Gly  Asn Arg Gln 
    1640                 1645                 1650             


Asn Met  Ile Val Glu Pro Asn  Tyr Asp Leu Asp Asp  Ser Gly Asp 
    1655                 1660                 1665             


Ile Ser  Ser Thr Val Ile Asn  Phe Ser Gln Lys Tyr  Leu Tyr Gly 
    1670                 1675                 1680             


Ile Asp  Ser Cys Val Asn Lys  Val Ile Ile Ser Pro  Asn Ile Tyr 
    1685                 1690                 1695             


Thr Asp  Glu Ile Asn Ile Thr  Pro Ile Tyr Glu Ala  Asn Asn Thr 
    1700                 1705                 1710             


Tyr Pro  Glu Val Ile Val Leu  Asp Thr Asn Tyr Ile  Ser Glu Lys 
    1715                 1720                 1725             


Ile Asn  Ile Asn Ile Asn Asp  Leu Ser Ile Arg Tyr  Val Trp Ser 
    1730                 1735                 1740             


Asn Asp  Gly Ser Asp Phe Ile  Leu Met Ser Thr Asp  Glu Glu Asn 
    1745                 1750                 1755             


Lys Val  Ser Gln Val Lys Ile  Arg Phe Thr Asn Val  Phe Lys Gly 
    1760                 1765                 1770             


Asn Thr  Ile Ser Asp Lys Ile  Ser Phe Asn Phe Ser  Asp Lys Gln 
    1775                 1780                 1785             


Asp Val  Ser Ile Asn Lys Val  Ile Ser Thr Phe Thr  Pro Ser Tyr 
    1790                 1795                 1800             


Tyr Val  Glu Gly Leu Leu Asn  Tyr Asp Leu Gly Leu  Ile Ser Leu 
    1805                 1810                 1815             


Tyr Asn  Glu Lys Phe Tyr Ile  Asn Asn Phe Gly Met  Met Val Ser 
    1820                 1825                 1830             


Gly Leu  Val Tyr Ile Asn Asp  Ser Leu Tyr Tyr Phe  Lys Pro Pro 
    1835                 1840                 1845             


Ile Lys  Asn Leu Ile Thr Gly  Phe Thr Thr Ile Gly  Asp Asp Lys 
    1850                 1855                 1860             


Tyr Tyr  Phe Asn Pro Asp Asn  Gly Gly Ala Ala Ser  Val Gly Glu 
    1865                 1870                 1875             


Thr Ile  Ile Asp Gly Lys Asn  Tyr Tyr Phe Ser Gln  Asn Gly Val 
    1880                 1885                 1890             


Leu Gln  Thr Gly Val Phe Ser  Thr Glu Asp Gly Phe  Lys Tyr Phe 
    1895                 1900                 1905             


Ala Pro  Ala Asp Thr Leu Asp  Glu Asn Leu Glu Gly  Glu Ala Ile 
    1910                 1915                 1920             


Asp Phe  Thr Gly Lys Leu Thr  Ile Asp Glu Asn Val  Tyr Tyr Phe 
    1925                 1930                 1935             


Gly Asp  Asn Tyr Arg Ala Ala  Ile Glu Trp Gln Thr  Leu Asp Asp 
    1940                 1945                 1950             


Glu Val  Tyr Tyr Phe Ser Thr  Asp Thr Gly Arg Ala  Phe Lys Gly 
    1955                 1960                 1965             


Leu Asn  Gln Ile Gly Asp Asp  Lys Phe Tyr Phe Asn  Ser Asp Gly 
    1970                 1975                 1980             


Ile Met  Gln Lys Gly Phe Val  Asn Ile Asn Asp Lys  Thr Phe Tyr 
    1985                 1990                 1995             


Phe Asp  Asp Ser Gly Val Met  Lys Ser Gly Tyr Thr  Glu Ile Asp 
    2000                 2005                 2010             


Gly Lys  Tyr Phe Tyr Phe Ala  Glu Asn Gly Glu Met  Gln Ile Gly 
    2015                 2020                 2025             


Val Phe  Asn Thr Ala Asp Gly  Phe Lys Tyr Phe Ala  His His Asp 
    2030                 2035                 2040             


Glu Asp  Leu Gly Asn Glu Glu  Gly Glu Ala Leu Ser  Tyr Ser Gly 
    2045                 2050                 2055             


Ile Leu  Asn Phe Asn Asn Lys  Ile Tyr Tyr Phe Asp  Asp Ser Phe 
    2060                 2065                 2070             


Thr Ala  Val Val Gly Trp Lys  Asp Leu Glu Asp Gly  Ser Lys Tyr 
    2075                 2080                 2085             


Tyr Phe  Asp Glu Asp Thr Ala  Glu Ala Tyr Ile Gly  Ile Ser Ile 
    2090                 2095                 2100             


Ile Asn  Asp Gly Lys Tyr Tyr  Phe Asn Asp Ser Gly  Ile Met Gln 
    2105                 2110                 2115             


Ile Gly  Phe Val Thr Ile Asn  Asn Glu Val Phe Tyr  Phe Ser Asp 
    2120                 2125                 2130             


Ser Gly  Ile Val Glu Ser Gly  Met Gln Asn Ile Asp  Asp Asn Tyr 
    2135                 2140                 2145             


Phe Tyr  Ile Asp Glu Asn Gly  Leu Val Gln Ile Gly  Val Phe Asp 
    2150                 2155                 2160             


Thr Ser  Asp Gly Tyr Lys Tyr  Phe Ala Pro Ala Asn  Thr Val Asn 
    2165                 2170                 2175             


Asp Asn  Ile Tyr Gly Gln Ala  Val Glu Tyr Ser Gly  Leu Val Arg 
    2180                 2185                 2190             


Val Gly  Glu Asp Val Tyr Tyr  Phe Gly Glu Thr Tyr  Thr Ile Glu 
    2195                 2200                 2205             


Thr Gly  Trp Ile Tyr Asp Met  Glu Asn Glu Ser Asp  Lys Tyr Tyr 
    2210                 2215                 2220             


Phe Asp  Pro Glu Thr Lys Lys  Ala Tyr Lys Gly Ile  Asn Val Ile 
    2225                 2230                 2235             


Asp Asp  Ile Lys Tyr Tyr Phe  Asp Glu Asn Gly Ile  Met Arg Thr 
    2240                 2245                 2250             


Gly Leu  Ile Thr Phe Glu Asp  Asn His Tyr Tyr Phe  Asn Glu Asp 
    2255                 2260                 2265             


Gly Ile  Met Gln Tyr Gly Tyr  Leu Asn Ile Glu Asp  Lys Thr Phe 
    2270                 2275                 2280             


Tyr Phe  Ser Glu Asp Gly Ile  Met Gln Ile Gly Val  Phe Asn Thr 
    2285                 2290                 2295             


Pro Asp  Gly Phe Lys Tyr Phe  Ala His Gln Asn Thr  Leu Asp Glu 
    2300                 2305                 2310             


Asn Phe  Glu Gly Glu Ser Ile  Asn Tyr Thr Gly Trp  Leu Asp Leu 
    2315                 2320                 2325             


Asp Glu  Lys Arg Tyr Tyr Phe  Thr Asp Glu Tyr Ile  Ala Ala Thr 
    2330                 2335                 2340             


Gly Ser  Val Ile Ile Asp Gly  Glu Glu Tyr Tyr Phe  Asp Pro Asp 
    2345                 2350                 2355             


Thr Ala  Gln Leu Val Ile Ser  Glu 
    2360                 2365     


<210>  14
<211>  2366
<212>  PRT
<213>  Clostridium difficile


<220>
<221>  PEPTIDE
<222>  (1)..(2366)

<400>  14

Met Ser Leu Val Asn Arg Lys Gln Leu Glu Lys Met Ala Asn Val Arg 
1               5                   10                  15      


Phe Arg Thr Gln Glu Asp Glu Tyr Val Ala Ile Leu Asp Ala Leu Glu 
            20                  25                  30          


Glu Tyr His Asn Met Ser Glu Asn Thr Val Val Glu Lys Tyr Leu Lys 
        35                  40                  45              


Leu Lys Asp Ile Asn Ser Leu Thr Asp Ile Tyr Ile Asp Thr Tyr Lys 
    50                  55                  60                  


Lys Ser Gly Arg Asn Lys Ala Leu Lys Lys Phe Lys Glu Tyr Leu Val 
65                  70                  75                  80  


Thr Glu Val Leu Glu Leu Lys Asn Asn Asn Leu Thr Pro Val Glu Lys 
                85                  90                  95      


Asn Leu His Phe Val Ala Ile Gly Gly Gln Ile Asn Asp Thr Ala Ile 
            100                 105                 110         


Asn Tyr Ile Asn Gln Trp Lys Asp Val Asn Ser Asp Tyr Asn Val Asn 
        115                 120                 125             


Val Phe Tyr Asp Ser Asn Ala Phe Leu Ile Asn Thr Leu Lys Lys Thr 
    130                 135                 140                 


Val Val Glu Ser Ala Ile Asn Asp Thr Leu Glu Ser Phe Arg Glu Asn 
145                 150                 155                 160 


Leu Asn Asp Pro Arg Phe Asp Tyr Asn Lys Phe Phe Arg Lys Arg Met 
                165                 170                 175     


Glu Ile Ile Tyr Asp Lys Gln Lys Asn Phe Ile Asn Tyr Tyr Lys Ala 
            180                 185                 190         


Gln Arg Glu Glu Asn Pro Glu Leu Ile Ile Asp Asp Ile Val Lys Thr 
        195                 200                 205             


Tyr Leu Ser Asn Glu Tyr Ser Lys Glu Ile Asp Glu Leu Asn Thr Tyr 
    210                 215                 220                 


Ile Glu Glu Ser Leu Asn Lys Ile Thr Gln Asn Ser Gly Asn Asp Val 
225                 230                 235                 240 


Arg Asn Phe Glu Glu Phe Lys Asn Gly Glu Ser Phe Asn Leu Tyr Glu 
                245                 250                 255     


Gln Glu Leu Val Glu Arg Trp Asn Leu Ala Ala Ala Ser Asp Ile Leu 
            260                 265                 270         


Arg Ile Ser Ala Leu Lys Glu Ile Gly Gly Met Tyr Leu Asp Val Asp 
        275                 280                 285             


Met Leu Pro Gly Ile Gln Pro Asp Leu Phe Glu Ser Ile Glu Lys Pro 
    290                 295                 300                 


Ser Ser Val Thr Val Asp Phe Trp Glu Met Thr Lys Leu Glu Ala Ile 
305                 310                 315                 320 


Met Lys Tyr Lys Glu Tyr Ile Pro Glu Tyr Thr Ser Glu His Phe Asp 
                325                 330                 335     


Met Leu Asp Glu Glu Val Gln Ser Ser Phe Glu Ser Val Leu Ala Ser 
            340                 345                 350         


Lys Ser Asp Lys Ser Glu Ile Phe Ser Ser Leu Gly Asp Met Glu Ala 
        355                 360                 365             


Ser Pro Leu Glu Val Lys Ile Ala Phe Asn Ser Lys Gly Ile Ile Asn 
    370                 375                 380                 


Gln Gly Leu Ile Ser Val Lys Asp Ser Tyr Cys Ser Asn Leu Ile Val 
385                 390                 395                 400 


Lys Gln Ile Glu Asn Arg Tyr Lys Ile Leu Asn Asn Ser Leu Asn Pro 
                405                 410                 415     


Ala Ile Ser Glu Asp Asn Asp Phe Asn Thr Thr Thr Asn Thr Phe Ile 
            420                 425                 430         


Asp Ser Ile Met Ala Glu Ala Asn Ala Asp Asn Gly Arg Phe Met Met 
        435                 440                 445             


Glu Leu Gly Lys Tyr Leu Arg Val Gly Phe Phe Pro Asp Val Lys Thr 
    450                 455                 460                 


Thr Ile Asn Leu Ser Gly Pro Glu Ala Tyr Ala Ala Ala Tyr Gln Asp 
465                 470                 475                 480 


Leu Leu Met Phe Lys Glu Gly Ser Met Asn Ile His Leu Ile Glu Ala 
                485                 490                 495     


Asp Leu Arg Asn Phe Glu Ile Ser Lys Thr Asn Ile Ser Gln Ser Thr 
            500                 505                 510         


Glu Gln Glu Met Ala Ser Leu Trp Ser Phe Asp Asp Ala Arg Ala Lys 
        515                 520                 525             


Ala Gln Phe Glu Glu Tyr Lys Arg Asn Tyr Phe Glu Gly Ser Leu Gly 
    530                 535                 540                 


Glu Asp Asp Asn Leu Asp Phe Ser Gln Asn Ile Val Val Asp Lys Glu 
545                 550                 555                 560 


Tyr Leu Leu Glu Lys Ile Ser Ser Leu Ala Arg Ser Ser Glu Arg Gly 
                565                 570                 575     


Tyr Ile His Tyr Ile Val Gln Leu Gln Gly Asp Lys Ile Ser Tyr Glu 
            580                 585                 590         


Ala Ala Cys Asn Leu Phe Ala Lys Thr Pro Tyr Asp Ser Val Leu Phe 
        595                 600                 605             


Gln Lys Asn Ile Glu Asp Ser Glu Ile Ala Tyr Tyr Tyr Asn Pro Gly 
    610                 615                 620                 


Asp Gly Glu Ile Gln Glu Ile Asp Lys Tyr Lys Ile Pro Ser Ile Ile 
625                 630                 635                 640 


Ser Asp Arg Pro Lys Ile Lys Leu Thr Phe Ile Gly His Gly Lys Asp 
                645                 650                 655     


Glu Phe Asn Thr Asp Ile Phe Ala Gly Phe Asp Val Asp Ser Leu Ser 
            660                 665                 670         


Thr Glu Ile Glu Ala Ala Ile Asp Leu Ala Lys Glu Asp Ile Ser Pro 
        675                 680                 685             


Lys Ser Ile Glu Ile Asn Leu Leu Gly Cys Asn Met Phe Ser Tyr Ser 
    690                 695                 700                 


Ile Asn Val Glu Glu Thr Tyr Pro Gly Lys Leu Leu Leu Lys Val Lys 
705                 710                 715                 720 


Asp Lys Ile Ser Glu Leu Met Pro Ser Ile Ser Gln Asp Ser Ile Ile 
                725                 730                 735     


Val Ser Ala Asn Gln Tyr Glu Val Arg Ile Asn Ser Glu Gly Arg Arg 
            740                 745                 750         


Glu Leu Leu Asp His Ser Gly Glu Trp Ile Asn Lys Glu Glu Ser Ile 
        755                 760                 765             


Ile Lys Asp Ile Ser Ser Lys Glu Tyr Ile Ser Phe Asn Pro Lys Glu 
    770                 775                 780                 


Asn Lys Ile Thr Val Lys Ser Lys Asn Leu Pro Glu Leu Ser Thr Leu 
785                 790                 795                 800 


Leu Gln Glu Ile Arg Asn Asn Ser Asn Ser Ser Asp Ile Glu Leu Glu 
                805                 810                 815     


Glu Lys Val Met Leu Thr Glu Cys Glu Ile Asn Val Ile Ser Asn Ile 
            820                 825                 830         


Asp Thr Gln Ile Val Glu Glu Arg Ile Glu Glu Ala Lys Asn Leu Thr 
        835                 840                 845             


Ser Asp Ser Ile Asn Tyr Ile Lys Asp Glu Phe Lys Leu Ile Glu Ser 
    850                 855                 860                 


Ile Ser Asp Ala Leu Cys Asp Leu Lys Gln Gln Asn Glu Leu Glu Asp 
865                 870                 875                 880 


Ser His Phe Ile Ser Phe Glu Asp Ile Ser Glu Thr Asp Glu Gly Phe 
                885                 890                 895     


Ser Ile Arg Phe Ile Asn Lys Glu Thr Gly Glu Ser Ile Phe Val Glu 
            900                 905                 910         


Thr Glu Lys Thr Ile Phe Ser Glu Tyr Ala Asn His Ile Thr Glu Glu 
        915                 920                 925             


Ile Ser Lys Ile Lys Gly Thr Ile Phe Asp Thr Val Asn Gly Lys Leu 
    930                 935                 940                 


Val Lys Lys Val Asn Leu Asp Thr Thr His Glu Val Asn Thr Leu Asn 
945                 950                 955                 960 


Ala Ala Phe Phe Ile Gln Ser Leu Ile Glu Tyr Asn Ser Ser Lys Glu 
                965                 970                 975     


Ser Leu Ser Asn Leu Ser Val Ala Met Lys Val Gln Val Tyr Ala Gln 
            980                 985                 990         


Leu Phe Ser Thr Gly Leu Asn Thr  Ile Thr Asp Ala Ala  Lys Val Val 
        995                 1000                 1005             


Glu Leu  Val Ser Thr Ala Leu  Asp Glu Thr Ile Asp  Leu Leu Pro 
    1010                 1015                 1020             


Thr Leu  Ser Glu Gly Leu Pro  Ile Ile Ala Thr Ile  Ile Asp Gly 
    1025                 1030                 1035             


Val Ser  Leu Gly Ala Ala Ile  Lys Glu Leu Ser Glu  Thr Ser Asp 
    1040                 1045                 1050             


Pro Leu  Leu Arg Gln Glu Ile  Glu Ala Lys Ile Gly  Ile Met Ala 
    1055                 1060                 1065             


Val Asn  Leu Thr Thr Ala Thr  Thr Ala Ile Ile Thr  Ser Ser Leu 
    1070                 1075                 1080             


Gly Ile  Ala Ser Gly Phe Ser  Ile Leu Leu Val Pro  Leu Ala Gly 
    1085                 1090                 1095             


Ile Ser  Ala Gly Ile Pro Ser  Leu Val Asn Asn Glu  Leu Val Leu 
    1100                 1105                 1110             


Arg Asp  Lys Ala Thr Lys Val  Val Asp Tyr Phe Lys  His Val Ser 
    1115                 1120                 1125             


Leu Val  Glu Thr Glu Gly Val  Phe Thr Leu Leu Asp  Asp Lys Ile 
    1130                 1135                 1140             


Met Met  Pro Gln Asp Asp Leu  Val Ile Ser Glu Ile  Asp Phe Asn 
    1145                 1150                 1155             


Asn Asn  Ser Ile Val Leu Gly  Lys Cys Glu Ile Trp  Arg Met Glu 
    1160                 1165                 1170             


Gly Gly  Ser Gly His Thr Val  Thr Asp Asp Ile Asp  His Phe Phe 
    1175                 1180                 1185             


Ser Ala  Pro Ser Ile Thr Tyr  Arg Glu Pro His Leu  Ser Ile Tyr 
    1190                 1195                 1200             


Asp Val  Leu Glu Val Gln Lys  Glu Glu Leu Asp Leu  Ser Lys Asp 
    1205                 1210                 1215             


Leu Met  Val Leu Pro Asn Ala  Pro Asn Arg Val Phe  Ala Trp Glu 
    1220                 1225                 1230             


Thr Gly  Trp Thr Pro Gly Leu  Arg Ser Leu Glu Asn  Asp Gly Thr 
    1235                 1240                 1245             


Lys Leu  Leu Asp Arg Ile Arg  Asp Asn Tyr Glu Gly  Glu Phe Tyr 
    1250                 1255                 1260             


Trp Arg  Tyr Phe Ala Phe Ile  Ala Asp Ala Leu Ile  Thr Thr Leu 
    1265                 1270                 1275             


Lys Pro  Arg Tyr Glu Asp Thr  Asn Ile Arg Ile Asn  Leu Asp Ser 
    1280                 1285                 1290             


Asn Thr  Arg Ser Phe Ile Val  Pro Ile Ile Thr Thr  Glu Tyr Ile 
    1295                 1300                 1305             


Arg Glu  Lys Leu Ser Tyr Ser  Phe Tyr Gly Ser Gly  Gly Thr Tyr 
    1310                 1315                 1320             


Ala Leu  Ser Leu Ser Gln Tyr  Asn Met Gly Ile Asn  Ile Glu Leu 
    1325                 1330                 1335             


Ser Glu  Ser Asp Val Trp Ile  Ile Asp Val Asp Asn  Val Val Arg 
    1340                 1345                 1350             


Asp Val  Thr Ile Glu Ser Asp  Lys Ile Lys Lys Gly  Asp Leu Ile 
    1355                 1360                 1365             


Glu Gly  Ile Leu Ser Thr Leu  Ser Ile Glu Glu Asn  Lys Ile Ile 
    1370                 1375                 1380             


Leu Asn  Ser His Glu Ile Asn  Phe Ser Gly Glu Val  Asn Gly Ser 
    1385                 1390                 1395             


Asn Gly  Phe Val Ser Leu Thr  Phe Ser Ile Leu Glu  Gly Ile Asn 
    1400                 1405                 1410             


Ala Ile  Ile Glu Val Asp Leu  Leu Ser Lys Ser Tyr  Lys Leu Leu 
    1415                 1420                 1425             


Ile Ser  Gly Glu Leu Lys Ile  Leu Met Leu Asn Ser  Asn His Ile 
    1430                 1435                 1440             


Gln Gln  Lys Ile Asp Tyr Ile  Gly Phe Asn Ser Glu  Leu Gln Lys 
    1445                 1450                 1455             


Asn Ile  Pro Tyr Ser Phe Val  Asp Ser Glu Gly Lys  Glu Asn Gly 
    1460                 1465                 1470             


Phe Ile  Asn Gly Ser Thr Lys  Glu Gly Leu Phe Val  Ser Glu Leu 
    1475                 1480                 1485             


Pro Asp  Val Val Leu Ile Ser  Lys Val Tyr Met Asp  Asp Ser Lys 
    1490                 1495                 1500             


Pro Ser  Phe Gly Tyr Tyr Ser  Asn Asn Leu Lys Asp  Val Lys Val 
    1505                 1510                 1515             


Ile Thr  Lys Asp Asn Val Asn  Ile Leu Thr Gly Tyr  Tyr Leu Lys 
    1520                 1525                 1530             


Asp Asp  Ile Lys Ile Ser Leu  Ser Leu Thr Leu Gln  Asp Glu Lys 
    1535                 1540                 1545             


Thr Ile  Lys Leu Asn Ser Val  His Leu Asp Glu Ser  Gly Val Ala 
    1550                 1555                 1560             


Glu Ile  Leu Lys Phe Met Asn  Arg Lys Gly Asn Thr  Asn Thr Ser 
    1565                 1570                 1575             


Asp Ser  Leu Met Ser Phe Leu  Glu Ser Met Asn Ile  Lys Ser Ile 
    1580                 1585                 1590             


Phe Val  Asn Phe Leu Gln Ser  Asn Ile Lys Phe Ile  Leu Asp Ala 
    1595                 1600                 1605             


Asn Phe  Ile Ile Ser Gly Thr  Thr Ser Ile Gly Gln  Phe Glu Phe 
    1610                 1615                 1620             


Ile Cys  Asp Glu Asn Asp Asn  Ile Gln Pro Tyr Phe  Ile Lys Phe 
    1625                 1630                 1635             


Asn Thr  Leu Glu Thr Asn Tyr  Thr Leu Tyr Val Gly  Asn Arg Gln 
    1640                 1645                 1650             


Asn Met  Ile Val Glu Pro Asn  Tyr Asp Leu Asp Asp  Ser Gly Asp 
    1655                 1660                 1665             


Ile Ser  Ser Thr Val Ile Asn  Phe Ser Gln Lys Tyr  Leu Tyr Gly 
    1670                 1675                 1680             


Ile Asp  Ser Cys Val Asn Lys  Val Val Ile Ser Pro  Asn Ile Tyr 
    1685                 1690                 1695             


Thr Asp  Glu Ile Asn Ile Thr  Pro Val Tyr Glu Thr  Asn Asn Thr 
    1700                 1705                 1710             


Tyr Pro  Glu Val Ile Val Leu  Asp Ala Asn Tyr Ile  Asn Glu Lys 
    1715                 1720                 1725             


Ile Asn  Val Asn Ile Asn Asp  Leu Ser Ile Arg Tyr  Val Trp Ser 
    1730                 1735                 1740             


Asn Asp  Gly Asn Asp Phe Ile  Leu Met Ser Thr Ser  Glu Glu Asn 
    1745                 1750                 1755             


Lys Val  Ser Gln Val Lys Ile  Arg Phe Val Asn Val  Phe Lys Asp 
    1760                 1765                 1770             


Lys Thr  Leu Ala Asn Lys Leu  Ser Phe Asn Phe Ser  Asp Lys Gln 
    1775                 1780                 1785             


Asp Val  Pro Val Ser Glu Ile  Ile Leu Ser Phe Thr  Pro Ser Tyr 
    1790                 1795                 1800             


Tyr Glu  Asp Gly Leu Ile Gly  Tyr Asp Leu Gly Leu  Val Ser Leu 
    1805                 1810                 1815             


Tyr Asn  Glu Lys Phe Tyr Ile  Asn Asn Phe Gly Met  Met Val Ser 
    1820                 1825                 1830             


Gly Leu  Ile Tyr Ile Asn Asp  Ser Leu Tyr Tyr Phe  Lys Pro Pro 
    1835                 1840                 1845             


Val Asn  Asn Leu Ile Thr Gly  Phe Val Thr Val Gly  Asp Asp Lys 
    1850                 1855                 1860             


Tyr Tyr  Phe Asn Pro Ile Asn  Gly Gly Ala Ala Ser  Ile Gly Glu 
    1865                 1870                 1875             


Thr Ile  Ile Asp Asp Lys Asn  Tyr Tyr Phe Asn Gln  Ser Gly Val 
    1880                 1885                 1890             


Leu Gln  Thr Gly Val Phe Ser  Thr Glu Asp Gly Phe  Lys Tyr Phe 
    1895                 1900                 1905             


Ala Pro  Ala Asn Thr Leu Asp  Glu Asn Leu Glu Gly  Glu Ala Ile 
    1910                 1915                 1920             


Asp Phe  Thr Gly Lys Leu Ile  Ile Asp Glu Asn Ile  Tyr Tyr Phe 
    1925                 1930                 1935             


Asp Asp  Asn Tyr Arg Gly Ala  Val Glu Trp Lys Glu  Leu Asp Gly 
    1940                 1945                 1950             


Glu Met  His Tyr Phe Ser Pro  Glu Thr Gly Lys Ala  Phe Lys Gly 
    1955                 1960                 1965             


Leu Asn  Gln Ile Gly Asp Tyr  Lys Tyr Tyr Phe Asn  Ser Asp Gly 
    1970                 1975                 1980             


Val Met  Gln Lys Gly Phe Val  Ser Ile Asn Asp Asn  Lys His Tyr 
    1985                 1990                 1995             


Phe Asp  Asp Ser Gly Val Met  Lys Val Gly Tyr Thr  Glu Ile Asp 
    2000                 2005                 2010             


Gly Lys  His Phe Tyr Phe Ala  Glu Asn Gly Glu Met  Gln Ile Gly 
    2015                 2020                 2025             


Val Phe  Asn Thr Glu Asp Gly  Phe Lys Tyr Phe Ala  His His Asn 
    2030                 2035                 2040             


Glu Asp  Leu Gly Asn Glu Glu  Gly Glu Glu Ile Ser  Tyr Ser Gly 
    2045                 2050                 2055             


Ile Leu  Asn Phe Asn Asn Lys  Ile Tyr Tyr Phe Asp  Asp Ser Phe 
    2060                 2065                 2070             


Thr Ala  Val Val Gly Trp Lys  Asp Leu Glu Asp Gly  Ser Lys Tyr 
    2075                 2080                 2085             


Tyr Phe  Asp Glu Asp Thr Ala  Glu Ala Tyr Ile Gly  Leu Ser Leu 
    2090                 2095                 2100             


Ile Asn  Asp Gly Gln Tyr Tyr  Phe Asn Asp Asp Gly  Ile Met Gln 
    2105                 2110                 2115             


Val Gly  Phe Val Thr Ile Asn  Asp Lys Val Phe Tyr  Phe Ser Asp 
    2120                 2125                 2130             


Ser Gly  Ile Ile Glu Ser Gly  Val Gln Asn Ile Asp  Asp Asn Tyr 
    2135                 2140                 2145             


Phe Tyr  Ile Asp Asp Asn Gly  Ile Val Gln Ile Gly  Val Phe Asp 
    2150                 2155                 2160             


Thr Ser  Asp Gly Tyr Lys Tyr  Phe Ala Pro Ala Asn  Thr Val Asn 
    2165                 2170                 2175             


Asp Asn  Ile Tyr Gly Gln Ala  Val Glu Tyr Ser Gly  Leu Val Arg 
    2180                 2185                 2190             


Val Gly  Glu Asp Val Tyr Tyr  Phe Gly Glu Thr Tyr  Thr Ile Glu 
    2195                 2200                 2205             


Thr Gly  Trp Ile Tyr Asp Met  Glu Asn Glu Ser Asp  Lys Tyr Tyr 
    2210                 2215                 2220             


Phe Asn  Pro Glu Thr Lys Lys  Ala Cys Lys Gly Ile  Asn Leu Ile 
    2225                 2230                 2235             


Asp Asp  Ile Lys Tyr Tyr Phe  Asp Glu Lys Gly Ile  Met Arg Thr 
    2240                 2245                 2250             


Gly Leu  Ile Ser Phe Glu Asn  Asn Asn Tyr Tyr Phe  Asn Glu Asn 
    2255                 2260                 2265             


Gly Glu  Met Gln Phe Gly Tyr  Ile Asn Ile Glu Asp  Lys Met Phe 
    2270                 2275                 2280             


Tyr Phe  Gly Glu Asp Gly Val  Met Gln Ile Gly Val  Phe Asn Thr 
    2285                 2290                 2295             


Pro Asp  Gly Phe Lys Tyr Phe  Ala His Gln Asn Thr  Leu Asp Glu 
    2300                 2305                 2310             


Asn Phe  Glu Gly Glu Ser Ile  Asn Tyr Thr Gly Trp  Leu Asp Leu 
    2315                 2320                 2325             


Asp Glu  Lys Arg Tyr Tyr Phe  Thr Asp Glu Tyr Ile  Ala Ala Thr 
    2330                 2335                 2340             


Gly Ser  Val Ile Ile Asp Gly  Glu Glu Tyr Tyr Phe  Asp Pro Asp 
    2345                 2350                 2355             


Thr Ala  Gln Leu Val Ile Ser  Glu 
    2360                 2365     


<210>  15
<211>  2366
<212>  PRT
<213>  Clostridium difficile


<220>
<221>  PEPTIDE
<222>  (1)..(2366)

<400>  15

Met Ser Leu Val Asn Arg Lys Gln Leu Glu Lys Met Ala Asn Val Arg 
1               5                   10                  15      


Phe Arg Thr Gln Glu Asp Glu Tyr Val Ala Ile Leu Asp Ala Leu Glu 
            20                  25                  30          


Glu Tyr His Asn Met Ser Glu Asn Thr Val Val Glu Lys Tyr Leu Lys 
        35                  40                  45              


Leu Lys Asp Ile Asn Ser Leu Thr Asp Ile Tyr Ile Asp Thr Tyr Lys 
    50                  55                  60                  


Lys Ser Gly Arg Asn Lys Ala Leu Lys Lys Phe Lys Glu Tyr Leu Val 
65                  70                  75                  80  


Thr Glu Val Leu Glu Leu Lys Asn Asn Asn Leu Thr Pro Val Glu Lys 
                85                  90                  95      


Asn Leu His Phe Val Trp Ile Gly Gly Gln Ile Asn Asp Thr Ala Ile 
            100                 105                 110         


Asn Tyr Ile Asn Gln Trp Lys Asp Val Asn Ser Asp Tyr Asn Val Asn 
        115                 120                 125             


Val Phe Tyr Asp Ser Asn Ala Phe Leu Ile Asn Thr Leu Lys Lys Thr 
    130                 135                 140                 


Val Val Glu Ser Ala Ile Asn Asp Thr Leu Glu Ser Phe Arg Glu Asn 
145                 150                 155                 160 


Leu Asn Asp Pro Arg Phe Asp Tyr Asn Lys Phe Phe Arg Lys Arg Met 
                165                 170                 175     


Glu Ile Ile Tyr Asp Lys Gln Lys Asn Phe Ile Asn Tyr Tyr Lys Ala 
            180                 185                 190         


Gln Arg Glu Glu Asn Pro Glu Leu Ile Ile Asp Asp Ile Val Lys Thr 
        195                 200                 205             


Tyr Leu Ser Asn Glu Tyr Ser Lys Glu Ile Asp Glu Leu Asn Thr Tyr 
    210                 215                 220                 


Ile Glu Glu Ser Leu Asn Lys Ile Thr Gln Asn Ser Gly Asn Asp Val 
225                 230                 235                 240 


Arg Asn Phe Glu Glu Phe Lys Asn Gly Glu Ser Phe Asn Leu Tyr Glu 
                245                 250                 255     


Gln Glu Leu Val Glu Arg Trp Asn Leu Ala Ala Ala Ser Asp Ile Leu 
            260                 265                 270         


Arg Ile Ser Ala Leu Lys Glu Ile Gly Gly Met Tyr Leu Gly Val Gly 
        275                 280                 285             


Met Leu Pro Gly Ile Gln Pro Asp Leu Phe Glu Ser Ile Glu Lys Pro 
    290                 295                 300                 


Ser Ser Val Thr Val Asp Phe Trp Glu Met Thr Lys Leu Glu Ala Ile 
305                 310                 315                 320 


Met Lys Tyr Lys Glu Tyr Ile Pro Glu Tyr Thr Ser Glu His Phe Asp 
                325                 330                 335     


Met Leu Asp Glu Glu Val Gln Ser Ser Phe Glu Ser Val Leu Ala Ser 
            340                 345                 350         


Lys Ser Asp Lys Ser Glu Ile Phe Ser Ser Leu Gly Asp Met Glu Ala 
        355                 360                 365             


Ser Pro Leu Glu Val Lys Ile Ala Phe Asn Ser Lys Gly Ile Ile Asn 
    370                 375                 380                 


Gln Gly Leu Ile Ser Val Lys Asp Ser Tyr Cys Ser Asn Leu Ile Val 
385                 390                 395                 400 


Lys Gln Ile Glu Asn Arg Tyr Lys Ile Leu Asn Asn Ser Leu Asn Pro 
                405                 410                 415     


Ala Ile Ser Glu Asp Asn Asp Phe Asn Thr Thr Thr Asn Thr Phe Ile 
            420                 425                 430         


Asp Ser Ile Met Ala Glu Ala Asn Ala Asp Asn Gly Arg Phe Met Met 
        435                 440                 445             


Glu Leu Gly Lys Tyr Leu Arg Val Gly Phe Phe Pro Asp Val Lys Thr 
    450                 455                 460                 


Thr Ile Asn Leu Ser Gly Pro Glu Ala Tyr Ala Ala Ala Tyr Gln Asp 
465                 470                 475                 480 


Leu Leu Met Phe Lys Glu Gly Ser Met Asn Ile His Leu Ile Glu Ala 
                485                 490                 495     


Asp Leu Arg Asn Phe Glu Ile Ser Lys Thr Asn Ile Ser Gln Ser Thr 
            500                 505                 510         


Glu Gln Glu Met Ala Ser Leu Trp Ser Phe Asp Asp Ala Arg Ala Lys 
        515                 520                 525             


Ala Gln Phe Glu Glu Tyr Lys Arg Asn Tyr Phe Glu Gly Ser Leu Gly 
    530                 535                 540                 


Glu Asp Asp Asn Leu Asp Phe Ser Gln Asn Ile Val Val Asp Lys Glu 
545                 550                 555                 560 


Tyr Leu Leu Glu Lys Ile Ser Ser Leu Ala Arg Ser Ser Glu Arg Gly 
                565                 570                 575     


Tyr Ile His Tyr Ile Val Gln Leu Gln Gly Asp Lys Ile Ser Tyr Glu 
            580                 585                 590         


Ala Ala Cys Asn Leu Phe Ala Lys Thr Pro Tyr Asp Ser Val Leu Phe 
        595                 600                 605             


Gln Lys Asn Ile Glu Asp Ser Glu Ile Ala Tyr Tyr Tyr Asn Pro Gly 
    610                 615                 620                 


Asp Gly Glu Ile Gln Glu Ile Asp Lys Tyr Lys Ile Pro Ser Ile Ile 
625                 630                 635                 640 


Ser Asp Arg Pro Lys Ile Lys Leu Thr Phe Ile Gly His Gly Lys Asp 
                645                 650                 655     


Glu Phe Asn Thr Asp Ile Phe Ala Gly Phe Asp Val Asp Ser Leu Ser 
            660                 665                 670         


Thr Glu Ile Glu Ala Ala Ile Asp Leu Ala Lys Glu Asp Ile Ser Pro 
        675                 680                 685             


Lys Ser Ile Glu Ile Asn Leu Leu Gly Cys Asn Met Phe Ser Tyr Ser 
    690                 695                 700                 


Ile Asn Val Glu Glu Thr Tyr Pro Gly Lys Leu Leu Leu Lys Val Lys 
705                 710                 715                 720 


Asp Lys Ile Ser Glu Leu Met Pro Ser Ile Ser Gln Asp Ser Ile Ile 
                725                 730                 735     


Val Ser Ala Asn Gln Tyr Glu Val Arg Ile Asn Ser Glu Gly Arg Arg 
            740                 745                 750         


Glu Leu Leu Asp His Ser Gly Glu Trp Ile Asn Lys Glu Glu Ser Ile 
        755                 760                 765             


Ile Lys Asp Ile Ser Ser Lys Glu Tyr Ile Ser Phe Asn Pro Lys Glu 
    770                 775                 780                 


Asn Lys Ile Thr Val Lys Ser Lys Asn Leu Pro Glu Leu Ser Thr Leu 
785                 790                 795                 800 


Leu Gln Glu Ile Arg Asn Asn Ser Asn Ser Ser Asp Ile Glu Leu Glu 
                805                 810                 815     


Glu Lys Val Met Leu Thr Glu Cys Glu Ile Asn Val Ile Ser Asn Ile 
            820                 825                 830         


Asp Thr Gln Ile Val Glu Glu Arg Ile Glu Glu Ala Lys Asn Leu Thr 
        835                 840                 845             


Ser Asp Ser Ile Asn Tyr Ile Lys Asp Glu Phe Lys Leu Ile Glu Ser 
    850                 855                 860                 


Ile Ser Asp Ala Leu Cys Asp Leu Lys Gln Gln Asn Glu Leu Glu Asp 
865                 870                 875                 880 


Ser His Phe Ile Ser Phe Glu Asp Ile Ser Glu Thr Asp Glu Gly Phe 
                885                 890                 895     


Ser Ile Arg Phe Ile Asn Lys Glu Thr Gly Glu Ser Ile Phe Val Glu 
            900                 905                 910         


Thr Glu Lys Thr Ile Phe Ser Glu Tyr Ala Asn His Ile Thr Glu Glu 
        915                 920                 925             


Ile Ser Lys Ile Lys Gly Thr Ile Phe Asp Thr Val Asn Gly Lys Leu 
    930                 935                 940                 


Val Lys Lys Val Asn Leu Asp Thr Thr His Glu Val Asn Thr Leu Asn 
945                 950                 955                 960 


Ala Ala Phe Phe Ile Gln Ser Leu Ile Glu Tyr Asn Ser Ser Lys Glu 
                965                 970                 975     


Ser Leu Ser Asn Leu Ser Val Ala Met Lys Val Gln Val Tyr Ala Gln 
            980                 985                 990         


Leu Phe Ser Thr Gly Leu Asn Thr  Ile Thr Asp Ala Ala  Lys Val Val 
        995                 1000                 1005             


Glu Leu  Val Ser Thr Ala Leu  Asp Glu Thr Ile Asp  Leu Leu Pro 
    1010                 1015                 1020             


Thr Leu  Ser Glu Gly Leu Pro  Ile Ile Ala Thr Ile  Ile Asp Gly 
    1025                 1030                 1035             


Val Ser  Leu Gly Ala Ala Ile  Lys Glu Leu Ser Glu  Thr Ser Asp 
    1040                 1045                 1050             


Pro Leu  Leu Arg Gln Glu Ile  Glu Ala Lys Ile Gly  Ile Met Ala 
    1055                 1060                 1065             


Val Asn  Leu Thr Thr Ala Thr  Thr Ala Ile Ile Thr  Ser Ser Leu 
    1070                 1075                 1080             


Gly Ile  Ala Ser Gly Phe Ser  Ile Leu Leu Val Pro  Leu Ala Gly 
    1085                 1090                 1095             


Ile Ser  Ala Gly Ile Pro Ser  Leu Val Asn Asn Glu  Leu Val Leu 
    1100                 1105                 1110             


Arg Asp  Lys Ala Thr Lys Val  Val Asp Tyr Phe Lys  His Val Ser 
    1115                 1120                 1125             


Leu Val  Glu Thr Glu Gly Val  Phe Thr Leu Leu Asp  Asp Lys Ile 
    1130                 1135                 1140             


Met Met  Pro Gln Asp Asp Leu  Val Ile Ser Glu Ile  Asp Phe Asn 
    1145                 1150                 1155             


Asn Asn  Ser Ile Val Leu Gly  Lys Cys Glu Ile Trp  Arg Met Glu 
    1160                 1165                 1170             


Gly Gly  Ser Gly His Thr Val  Thr Asp Asp Ile Asp  His Phe Phe 
    1175                 1180                 1185             


Ser Ala  Pro Ser Ile Thr Tyr  Arg Glu Pro His Leu  Ser Ile Tyr 
    1190                 1195                 1200             


Asp Val  Leu Glu Val Gln Lys  Glu Glu Leu Asp Leu  Ser Lys Asp 
    1205                 1210                 1215             


Leu Met  Val Leu Pro Asn Ala  Pro Asn Arg Val Phe  Ala Trp Glu 
    1220                 1225                 1230             


Thr Gly  Trp Thr Pro Gly Leu  Arg Ser Leu Glu Asn  Asp Gly Thr 
    1235                 1240                 1245             


Lys Leu  Leu Asp Arg Ile Arg  Asp Asn Tyr Glu Gly  Glu Phe Tyr 
    1250                 1255                 1260             


Trp Arg  Tyr Phe Ala Phe Ile  Ala Asp Ala Leu Ile  Thr Thr Leu 
    1265                 1270                 1275             


Lys Pro  Arg Tyr Glu Asp Thr  Asn Ile Arg Ile Asn  Leu Asp Ser 
    1280                 1285                 1290             


Asn Thr  Arg Ser Phe Ile Val  Pro Ile Ile Thr Thr  Glu Tyr Ile 
    1295                 1300                 1305             


Arg Glu  Lys Leu Ser Tyr Ser  Phe Tyr Gly Ser Gly  Gly Thr Tyr 
    1310                 1315                 1320             


Ala Leu  Ser Leu Ser Gln Tyr  Asn Met Gly Ile Asn  Ile Glu Leu 
    1325                 1330                 1335             


Ser Glu  Ser Asp Val Trp Ile  Ile Asp Val Asp Asn  Val Val Arg 
    1340                 1345                 1350             


Asp Val  Thr Ile Glu Ser Asp  Lys Ile Lys Lys Gly  Asp Leu Ile 
    1355                 1360                 1365             


Glu Gly  Ile Leu Ser Thr Leu  Ser Ile Glu Glu Asn  Lys Ile Ile 
    1370                 1375                 1380             


Leu Asn  Ser His Glu Ile Asn  Phe Ser Gly Glu Val  Asn Gly Ser 
    1385                 1390                 1395             


Asn Gly  Phe Val Ser Leu Thr  Phe Ser Ile Leu Glu  Gly Ile Asn 
    1400                 1405                 1410             


Ala Ile  Ile Glu Val Asp Leu  Leu Ser Lys Ser Tyr  Lys Leu Leu 
    1415                 1420                 1425             


Ile Ser  Gly Glu Leu Lys Ile  Leu Met Leu Asn Ser  Asn His Ile 
    1430                 1435                 1440             


Gln Gln  Lys Ile Asp Tyr Ile  Gly Phe Asn Ser Glu  Leu Gln Lys 
    1445                 1450                 1455             


Asn Ile  Pro Tyr Ser Phe Val  Asp Ser Glu Gly Lys  Glu Asn Gly 
    1460                 1465                 1470             


Phe Ile  Asn Gly Ser Thr Lys  Glu Gly Leu Phe Val  Ser Glu Leu 
    1475                 1480                 1485             


Pro Asp  Val Val Leu Ile Ser  Lys Val Tyr Met Asp  Asp Ser Lys 
    1490                 1495                 1500             


Pro Ser  Phe Gly Tyr Tyr Ser  Asn Asn Leu Lys Asp  Val Lys Val 
    1505                 1510                 1515             


Ile Thr  Lys Asp Asn Val Asn  Ile Leu Thr Gly Tyr  Tyr Leu Lys 
    1520                 1525                 1530             


Asp Asp  Ile Lys Ile Ser Leu  Ser Leu Thr Leu Gln  Asp Glu Lys 
    1535                 1540                 1545             


Thr Ile  Lys Leu Asn Ser Val  His Leu Asp Glu Ser  Gly Val Ala 
    1550                 1555                 1560             


Glu Ile  Leu Lys Phe Met Asn  Arg Lys Gly Asn Thr  Asn Thr Ser 
    1565                 1570                 1575             


Asp Ser  Leu Met Ser Phe Leu  Glu Ser Met Asn Ile  Lys Ser Ile 
    1580                 1585                 1590             


Phe Val  Asn Phe Leu Gln Ser  Asn Ile Lys Phe Ile  Leu Asp Ala 
    1595                 1600                 1605             


Asn Phe  Ile Ile Ser Gly Thr  Thr Ser Ile Gly Gln  Phe Glu Phe 
    1610                 1615                 1620             


Ile Cys  Asp Glu Asn Asp Asn  Ile Gln Pro Tyr Phe  Ile Lys Phe 
    1625                 1630                 1635             


Asn Thr  Leu Glu Thr Asn Tyr  Thr Leu Tyr Val Gly  Asn Arg Gln 
    1640                 1645                 1650             


Asn Met  Ile Val Glu Pro Asn  Tyr Asp Leu Asp Asp  Ser Gly Asp 
    1655                 1660                 1665             


Ile Ser  Ser Thr Val Ile Asn  Phe Ser Gln Lys Tyr  Leu Tyr Gly 
    1670                 1675                 1680             


Ile Asp  Ser Cys Val Asn Lys  Val Val Ile Ser Pro  Asn Ile Tyr 
    1685                 1690                 1695             


Thr Asp  Glu Ile Asn Ile Thr  Pro Val Tyr Glu Thr  Asn Asn Thr 
    1700                 1705                 1710             


Tyr Pro  Glu Val Ile Val Leu  Asp Ala Asn Tyr Ile  Asn Glu Lys 
    1715                 1720                 1725             


Ile Asn  Val Asn Ile Asn Asp  Leu Ser Ile Arg Tyr  Val Trp Ser 
    1730                 1735                 1740             


Asn Asp  Gly Asn Asp Phe Ile  Leu Met Ser Thr Ser  Glu Glu Asn 
    1745                 1750                 1755             


Lys Val  Ser Gln Val Lys Ile  Arg Phe Val Asn Val  Phe Lys Asp 
    1760                 1765                 1770             


Lys Thr  Leu Ala Asn Lys Leu  Ser Phe Asn Phe Ser  Asp Lys Gln 
    1775                 1780                 1785             


Asp Val  Pro Val Ser Glu Ile  Ile Leu Ser Phe Thr  Pro Ser Tyr 
    1790                 1795                 1800             


Tyr Glu  Asp Gly Leu Ile Gly  Tyr Asp Leu Gly Leu  Val Ser Leu 
    1805                 1810                 1815             


Tyr Asn  Glu Lys Phe Tyr Ile  Asn Asn Phe Gly Met  Met Val Ser 
    1820                 1825                 1830             


Gly Leu  Ile Tyr Ile Asn Asp  Ser Leu Tyr Tyr Phe  Lys Pro Pro 
    1835                 1840                 1845             


Val Asn  Asn Leu Ile Thr Gly  Phe Val Thr Val Gly  Asp Asp Lys 
    1850                 1855                 1860             


Tyr Tyr  Phe Asn Pro Ile Asn  Gly Gly Ala Ala Ser  Ile Gly Glu 
    1865                 1870                 1875             


Thr Ile  Ile Asp Asp Lys Asn  Tyr Tyr Phe Asn Gln  Ser Gly Val 
    1880                 1885                 1890             


Leu Gln  Thr Gly Val Phe Ser  Thr Glu Asp Gly Phe  Lys Tyr Phe 
    1895                 1900                 1905             


Ala Pro  Ala Asn Thr Leu Asp  Glu Asn Leu Glu Gly  Glu Ala Ile 
    1910                 1915                 1920             


Asp Phe  Thr Gly Lys Leu Ile  Ile Asp Glu Asn Ile  Tyr Tyr Phe 
    1925                 1930                 1935             


Asp Asp  Asn Tyr Arg Gly Ala  Val Glu Trp Lys Glu  Leu Asp Gly 
    1940                 1945                 1950             


Glu Met  His Tyr Phe Ser Pro  Glu Thr Gly Lys Ala  Phe Lys Gly 
    1955                 1960                 1965             


Leu Asn  Gln Ile Gly Asp Tyr  Lys Tyr Tyr Phe Asn  Ser Asp Gly 
    1970                 1975                 1980             


Val Met  Gln Lys Gly Phe Val  Ser Ile Asn Asp Asn  Lys His Tyr 
    1985                 1990                 1995             


Phe Asp  Asp Ser Gly Val Met  Lys Val Gly Tyr Thr  Glu Ile Asp 
    2000                 2005                 2010             


Gly Lys  His Phe Tyr Phe Ala  Glu Asn Gly Glu Met  Gln Ile Gly 
    2015                 2020                 2025             


Val Phe  Asn Thr Glu Asp Gly  Phe Lys Tyr Phe Ala  His His Asn 
    2030                 2035                 2040             


Glu Asp  Leu Gly Asn Glu Glu  Gly Glu Glu Ile Ser  Tyr Ser Gly 
    2045                 2050                 2055             


Ile Leu  Asn Phe Asn Asn Lys  Ile Tyr Tyr Phe Asp  Asp Ser Phe 
    2060                 2065                 2070             


Thr Ala  Val Val Gly Trp Lys  Asp Leu Glu Asp Gly  Ser Lys Tyr 
    2075                 2080                 2085             


Tyr Phe  Asp Glu Asp Thr Ala  Glu Ala Tyr Ile Gly  Leu Ser Leu 
    2090                 2095                 2100             


Ile Asn  Asp Gly Gln Tyr Tyr  Phe Asn Asp Asp Gly  Ile Met Gln 
    2105                 2110                 2115             


Val Gly  Phe Val Thr Ile Asn  Asp Lys Val Phe Tyr  Phe Ser Asp 
    2120                 2125                 2130             


Ser Gly  Ile Ile Glu Ser Gly  Val Gln Asn Ile Asp  Asp Asn Tyr 
    2135                 2140                 2145             


Phe Tyr  Ile Asp Asp Asn Gly  Ile Val Gln Ile Gly  Val Phe Asp 
    2150                 2155                 2160             


Thr Ser  Asp Gly Tyr Lys Tyr  Phe Ala Pro Ala Asn  Thr Val Asn 
    2165                 2170                 2175             


Asp Asn  Ile Tyr Gly Gln Ala  Val Glu Tyr Ser Gly  Leu Val Arg 
    2180                 2185                 2190             


Val Gly  Glu Asp Val Tyr Tyr  Phe Gly Glu Thr Tyr  Thr Ile Glu 
    2195                 2200                 2205             


Thr Gly  Trp Ile Tyr Asp Met  Glu Asn Glu Ser Asp  Lys Tyr Tyr 
    2210                 2215                 2220             


Phe Asn  Pro Glu Thr Lys Lys  Ala Cys Lys Gly Ile  Asn Leu Ile 
    2225                 2230                 2235             


Asp Asp  Ile Lys Tyr Tyr Phe  Asp Glu Lys Gly Ile  Met Arg Thr 
    2240                 2245                 2250             


Gly Leu  Ile Ser Phe Glu Asn  Asn Asn Tyr Tyr Phe  Asn Glu Asn 
    2255                 2260                 2265             


Gly Glu  Met Gln Phe Gly Tyr  Ile Asn Ile Glu Asp  Lys Met Phe 
    2270                 2275                 2280             


Tyr Phe  Gly Glu Asp Gly Val  Met Gln Ile Gly Val  Phe Asn Thr 
    2285                 2290                 2295             


Pro Asp  Gly Phe Lys Tyr Phe  Ala His Gln Asn Thr  Leu Asp Glu 
    2300                 2305                 2310             


Asn Phe  Glu Gly Glu Ser Ile  Asn Tyr Thr Gly Trp  Leu Asp Leu 
    2315                 2320                 2325             


Asp Glu  Lys Arg Tyr Tyr Phe  Thr Asp Glu Tyr Ile  Ala Ala Thr 
    2330                 2335                 2340             


Gly Ser  Val Ile Ile Asp Gly  Glu Glu Tyr Tyr Phe  Asp Pro Asp 
    2345                 2350                 2355             


Thr Ala  Gln Leu Val Ile Ser  Glu 
    2360                 2365     


<210>  16
<211>  721
<212>  PRT
<213>  Clostridium difficile


<220>
<221>  PEPTIDE
<222>  (1)..(721)

<400>  16

Met Leu Tyr Val Gly Asn Arg Gln Asn Met Ile Val Glu Pro Asn Tyr 
1               5                   10                  15      


Asp Leu Asp Asp Ser Gly Asp Ile Ser Ser Thr Val Ile Asn Phe Ser 
            20                  25                  30          


Gln Lys Tyr Leu Tyr Gly Ile Asp Ser Cys Val Asn Lys Val Ile Ile 
        35                  40                  45              


Ser Pro Asn Ile Tyr Thr Asp Glu Ile Asn Ile Thr Pro Ile Tyr Glu 
    50                  55                  60                  


Ala Asn Asn Thr Tyr Pro Glu Val Ile Val Leu Asp Thr Asn Tyr Ile 
65                  70                  75                  80  


Ser Glu Lys Ile Asn Ile Asn Ile Asn Asp Leu Ser Ile Arg Tyr Val 
                85                  90                  95      


Trp Ser Asn Asp Gly Ser Asp Phe Ile Leu Met Ser Thr Asp Glu Glu 
            100                 105                 110         


Asn Lys Val Ser Gln Val Lys Ile Arg Phe Thr Asn Val Phe Lys Gly 
        115                 120                 125             


Asn Thr Ile Ser Asp Lys Ile Ser Phe Asn Phe Ser Asp Lys Gln Asp 
    130                 135                 140                 


Val Ser Ile Asn Lys Val Ile Ser Thr Phe Thr Pro Ser Tyr Tyr Val 
145                 150                 155                 160 


Glu Gly Leu Leu Asn Tyr Asp Leu Gly Leu Ile Ser Leu Tyr Asn Glu 
                165                 170                 175     


Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Val Tyr 
            180                 185                 190         


Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Ile Lys Asn Leu Ile 
        195                 200                 205             


Thr Gly Phe Thr Thr Ile Gly Asp Asp Lys Tyr Tyr Phe Asn Pro Asp 
    210                 215                 220                 


Asn Gly Gly Ala Ala Ser Val Gly Glu Thr Ile Ile Asp Gly Lys Asn 
225                 230                 235                 240 


Tyr Tyr Phe Ser Gln Asn Gly Val Leu Gln Thr Gly Val Phe Ser Thr 
                245                 250                 255     


Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asp Thr Leu Asp Glu Asn 
            260                 265                 270         


Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Thr Ile Asp Glu 
        275                 280                 285             


Asn Val Tyr Tyr Phe Gly Asp Asn Tyr Arg Ala Ala Ile Glu Trp Gln 
    290                 295                 300                 


Thr Leu Asp Asp Glu Val Tyr Tyr Phe Ser Thr Asp Thr Gly Arg Ala 
305                 310                 315                 320 


Phe Lys Gly Leu Asn Gln Ile Gly Asp Asp Lys Phe Tyr Phe Asn Ser 
                325                 330                 335     


Asp Gly Ile Met Gln Lys Gly Phe Val Asn Ile Asn Asp Lys Thr Phe 
            340                 345                 350         


Tyr Phe Asp Asp Ser Gly Val Met Lys Ser Gly Tyr Thr Glu Ile Asp 
        355                 360                 365             


Gly Lys Tyr Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val 
    370                 375                 380                 


Phe Asn Thr Ala Asp Gly Phe Lys Tyr Phe Ala His His Asp Glu Asp 
385                 390                 395                 400 


Leu Gly Asn Glu Glu Gly Glu Ala Leu Ser Tyr Ser Gly Ile Leu Asn 
                405                 410                 415     


Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val 
            420                 425                 430         


Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp 
        435                 440                 445             


Thr Ala Glu Ala Tyr Ile Gly Ile Ser Ile Ile Asn Asp Gly Lys Tyr 
    450                 455                 460                 


Tyr Phe Asn Asp Ser Gly Ile Met Gln Ile Gly Phe Val Thr Ile Asn 
465                 470                 475                 480 


Asn Glu Val Phe Tyr Phe Ser Asp Ser Gly Ile Val Glu Ser Gly Met 
                485                 490                 495     


Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Glu Asn Gly Leu Val 
            500                 505                 510         


Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro 
        515                 520                 525             


Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser 
    530                 535                 540                 


Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr 
545                 550                 555                 560 


Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys 
                565                 570                 575     


Tyr Tyr Phe Asp Pro Glu Thr Lys Lys Ala Tyr Lys Gly Ile Asn Val 
            580                 585                 590         


Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Asn Gly Ile Met Arg Thr 
        595                 600                 605             


Gly Leu Ile Thr Phe Glu Asp Asn His Tyr Tyr Phe Asn Glu Asp Gly 
    610                 615                 620                 


Ile Met Gln Tyr Gly Tyr Leu Asn Ile Glu Asp Lys Thr Phe Tyr Phe 
625                 630                 635                 640 


Ser Glu Asp Gly Ile Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly 
                645                 650                 655     


Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly 
            660                 665                 670         


Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr 
        675                 680                 685             


Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp 
    690                 695                 700                 


Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser 
705                 710                 715                 720 


Glu 
    


<210>  17
<211>  721
<212>  PRT
<213>  Clostridium difficile


<220>
<221>  PEPTIDE
<222>  (1)..(721)

<400>  17

Met Leu Tyr Val Gly Asn Arg Gln Asn Met Ile Val Glu Pro Asn Tyr 
1               5                   10                  15      


Asp Leu Asp Asp Ser Gly Asp Ile Ser Ser Thr Val Ile Asn Phe Ser 
            20                  25                  30          


Gln Lys Tyr Leu Tyr Gly Ile Asp Ser Cys Val Asn Lys Val Val Ile 
        35                  40                  45              


Ser Pro Asn Ile Tyr Thr Asp Glu Ile Asn Ile Thr Pro Val Tyr Glu 
    50                  55                  60                  


Thr Asn Asn Thr Tyr Pro Glu Val Ile Val Leu Asp Ala Asn Tyr Ile 
65                  70                  75                  80  


Asn Glu Lys Ile Asn Val Asn Ile Asn Asp Leu Ser Ile Arg Tyr Val 
                85                  90                  95      


Trp Ser Asn Asp Gly Asn Asp Phe Ile Leu Met Ser Thr Ser Glu Glu 
            100                 105                 110         


Asn Lys Val Ser Gln Val Lys Ile Arg Phe Val Asn Val Phe Lys Asp 
        115                 120                 125             


Lys Thr Leu Ala Asn Lys Leu Ser Phe Asn Phe Ser Asp Lys Gln Asp 
    130                 135                 140                 


Val Pro Val Ser Glu Ile Ile Leu Ser Phe Thr Pro Ser Tyr Tyr Glu 
145                 150                 155                 160 


Asp Gly Leu Ile Gly Tyr Asp Leu Gly Leu Val Ser Leu Tyr Asn Glu 
                165                 170                 175     


Lys Phe Tyr Ile Asn Asn Phe Gly Met Met Val Ser Gly Leu Ile Tyr 
            180                 185                 190         


Ile Asn Asp Ser Leu Tyr Tyr Phe Lys Pro Pro Val Asn Asn Leu Ile 
        195                 200                 205             


Thr Gly Phe Val Thr Val Gly Asp Asp Lys Tyr Tyr Phe Asn Pro Ile 
    210                 215                 220                 


Asn Gly Gly Ala Ala Ser Ile Gly Glu Thr Ile Ile Asp Asp Lys Asn 
225                 230                 235                 240 


Tyr Tyr Phe Asn Gln Ser Gly Val Leu Gln Thr Gly Val Phe Ser Thr 
                245                 250                 255     


Glu Asp Gly Phe Lys Tyr Phe Ala Pro Ala Asn Thr Leu Asp Glu Asn 
            260                 265                 270         


Leu Glu Gly Glu Ala Ile Asp Phe Thr Gly Lys Leu Ile Ile Asp Glu 
        275                 280                 285             


Asn Ile Tyr Tyr Phe Asp Asp Asn Tyr Arg Gly Ala Val Glu Trp Lys 
    290                 295                 300                 


Glu Leu Asp Gly Glu Met His Tyr Phe Ser Pro Glu Thr Gly Lys Ala 
305                 310                 315                 320 


Phe Lys Gly Leu Asn Gln Ile Gly Asp Tyr Lys Tyr Tyr Phe Asn Ser 
                325                 330                 335     


Asp Gly Val Met Gln Lys Gly Phe Val Ser Ile Asn Asp Asn Lys His 
            340                 345                 350         


Tyr Phe Asp Asp Ser Gly Val Met Lys Val Gly Tyr Thr Glu Ile Asp 
        355                 360                 365             


Gly Lys His Phe Tyr Phe Ala Glu Asn Gly Glu Met Gln Ile Gly Val 
    370                 375                 380                 


Phe Asn Thr Glu Asp Gly Phe Lys Tyr Phe Ala His His Asn Glu Asp 
385                 390                 395                 400 


Leu Gly Asn Glu Glu Gly Glu Glu Ile Ser Tyr Ser Gly Ile Leu Asn 
                405                 410                 415     


Phe Asn Asn Lys Ile Tyr Tyr Phe Asp Asp Ser Phe Thr Ala Val Val 
            420                 425                 430         


Gly Trp Lys Asp Leu Glu Asp Gly Ser Lys Tyr Tyr Phe Asp Glu Asp 
        435                 440                 445             


Thr Ala Glu Ala Tyr Ile Gly Leu Ser Leu Ile Asn Asp Gly Gln Tyr 
    450                 455                 460                 


Tyr Phe Asn Asp Asp Gly Ile Met Gln Val Gly Phe Val Thr Ile Asn 
465                 470                 475                 480 


Asp Lys Val Phe Tyr Phe Ser Asp Ser Gly Ile Ile Glu Ser Gly Val 
                485                 490                 495     


Gln Asn Ile Asp Asp Asn Tyr Phe Tyr Ile Asp Asp Asn Gly Ile Val 
            500                 505                 510         


Gln Ile Gly Val Phe Asp Thr Ser Asp Gly Tyr Lys Tyr Phe Ala Pro 
        515                 520                 525             


Ala Asn Thr Val Asn Asp Asn Ile Tyr Gly Gln Ala Val Glu Tyr Ser 
    530                 535                 540                 


Gly Leu Val Arg Val Gly Glu Asp Val Tyr Tyr Phe Gly Glu Thr Tyr 
545                 550                 555                 560 


Thr Ile Glu Thr Gly Trp Ile Tyr Asp Met Glu Asn Glu Ser Asp Lys 
                565                 570                 575     


Tyr Tyr Phe Asn Pro Glu Thr Lys Lys Ala Cys Lys Gly Ile Asn Leu 
            580                 585                 590         


Ile Asp Asp Ile Lys Tyr Tyr Phe Asp Glu Lys Gly Ile Met Arg Thr 
        595                 600                 605             


Gly Leu Ile Ser Phe Glu Asn Asn Asn Tyr Tyr Phe Asn Glu Asn Gly 
    610                 615                 620                 


Glu Met Gln Phe Gly Tyr Ile Asn Ile Glu Asp Lys Met Phe Tyr Phe 
625                 630                 635                 640 


Gly Glu Asp Gly Val Met Gln Ile Gly Val Phe Asn Thr Pro Asp Gly 
                645                 650                 655     


Phe Lys Tyr Phe Ala His Gln Asn Thr Leu Asp Glu Asn Phe Glu Gly 
            660                 665                 670         


Glu Ser Ile Asn Tyr Thr Gly Trp Leu Asp Leu Asp Glu Lys Arg Tyr 
        675                 680                 685             


Tyr Phe Thr Asp Glu Tyr Ile Ala Ala Thr Gly Ser Val Ile Ile Asp 
    690                 695                 700                 


Gly Glu Glu Tyr Tyr Phe Asp Pro Asp Thr Ala Gln Leu Val Ile Ser 
705                 710                 715                 720 


Glu 
    


