                                SEQUENCE LISTING

<110> Ordway, Jared
      Bacher, Blaire
      Orion Genomics LLC

<120> Improved Detection of Nucleic Acid
  Sequences Adjacent to Repeated Sequences
  

<130> 86894-817857

<140> WO Not yet assigned 
<141> Not yet assigned  

<150> US 61/379,197       
<151> 2010-09-01  

<160> 27

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a 143 base pair
      amplicon within the promoter region of the Fragile
      Mental Retardation-1 (FMR-1) gene

<400> 1
gtcaccgccc ttcagccttc                                             20

<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a 143 base pair
      amplicon within the promoter region of the Fragile
      Mental Retardation-1 (FMR-1) gene

<400> 2
gccccgccct ctctcttcaa g                                           21

<210> 3
<211> 245
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon FMR1 A1 from Fragile Mental
      Retardation-1 (FMR-1) promoter region

<400> 3
agagaggccg aactgggata accggatgca tttgatttcc cacgccactg agtgcacctc 60
tgcagaaatg ggcgttctgg ccctcgcgag gcagtgcgac ctgtcaccgc ccttcagcct 120
tcccgccctc caccaagccc gcgcacgccc ggcccgcgcg tctgtctttc gacccggcac 180
cccggccggt tcccagcagc gcgcatgcgc gcgctcccag gccacttgaa gagagagggc 240
ggggc                                                             245

<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a 143 base pair
      amplicon (FMR1 A2) of the Fragile Mental Retardation-1
      (FMR-1) locus that lies downstream of the CCG repeat
      region

<400> 4
caccaaatca caatggcaac                                             20

<210> 5
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a 143 base pair
      amplicon (FMR1 A2) of the Fragile Mental Retardation-1
      (FMR-1) locus that lies downstream of the CCG repeat
      region

<400> 5
gtagcagcgc tgctaatgg                                              19

<210> 6
<211> 215
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon FMR1 A2 from the Fragile Mental
      Retardation-1 (FMR-1) locus that lies 681 base pairs
      downstream of the 3' end of the CCG repeat region

<400> 6
caccaaatca caatggcaac tgatttttag tggcttctct ttgtggattt cggaggagat 60
tttagatcca aaagtttcag gaagacccta acatggccca gcagtgcatt gaagaagttg 120
atcatcgtga atattcgcgt cccccttttt gttaaacggg gtaaattcag gaatgcacat 180
gcttcagcgt ctaaaaccat tagcagcgct gctac                            215

<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US1
      approximately 1 Kb upstream of the CCG repeat
      region

<400> 7
ggtactaagt tcaatgctgg c                                           21

<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US1
      approximately 1 Kb upstream of the CCG repeat
      region

<400> 8
gatgcacctc cttgcaaccc                                             20

<210> 9
<211> 248
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon US1 from the Fragile Mental
      Retardation-1 (FMR-1) locus 986 base pairs
      upstream of the 5' end of the CCG repeat region

<400> 9
ggtactaagt tcaatgctgg cagtcgtttc ttcttttttt ttcttttctg ttttgctcac 60
cgatttctcg ttagcactta gcacagtgtc tggcacacga tagatgctcc gtcaacttct 120
cagttggata ccagcatccc gaagggaaca tggattaagg cagctataag cacggtgtaa 180
aaacaggaat aagaaaaagt tgaggtttgt ttcacagtgg aatgtaaagg gttgcaagga 240
ggtgcatc                                                          248

<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US2
      approximately 2 Kb upstream of the CCG repeat
      region

<400> 10
gcatactcgg tagcaaacta g                                           21

<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US2
      approximately 2 Kb upstream of the CCG repeat
      region

<400> 11
cagtccctac agggtccttg                                             20

<210> 12
<211> 279
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon US2 from the Fragile Mental
      Retardation-1 (FMR-1) locus 1952 base pairs
      upstream of the 5' end of the CCG repeat region

<400> 12
gcatactcgg tagcaaacta ggtgtacaca cagcaatgtg ggtatagctc aaaaacagac 60
tgttgagtaa aacagtggga aatagagatt tacagtccaa taccatctct gtaaatgcaa 120
gaggcataaa caaaacatta tctgtgttaa attatcaagg atctctatcg aacatattgc 180
agcttgtgtc tagaagaatg agagtgggga tcgagaaaga tgaggaaaaa ataatataaa 240
cactataaaa taatgtaaac aaggaccctg tagggactg                        279

<210> 13
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US3
      approximately 3 Kb upstream of the CCG repeat
      region

<400> 13
catgcactgg aatgaagtga ag                                          22

<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US3
      approximately 3 Kb upstream of the CCG repeat
      region

<400> 14
cttcgctggt atccctgcag                                             20

<210> 15
<211> 256
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon US3 from the Fragile Mental
      Retardation-1 (FMR-1) locus 2984 base pairs
      upstream of the 5' end of the CCG repeat region

<400> 15
catgcactgg aatgaagtga agggggaaag tagaaggtga gatagcggag ataacagaag 60
tcagagtata tagggcactg aagccagaat aaataatgac tctggctttt actctgagag 120
atgtagggag tcactggggg attgtgagtg gcagagtgac ttgattgaca cctcaacagg 180
gtcattctgt cttctgtgct gagaactgtc agagagggta caacagtgga cagtgactgc 240
agggatacca gcgaag                                                 256

<210> 16
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US4
      approximately 4 Kb upstream of the CCG repeat
      region

<400> 16
ctgctgacac tgtaatggtg g                                           21

<210> 17
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US4
      approximately 4 Kb upstream of the CCG repeat
      region

<400> 17
ctttcttgac actttctctg ac                                          22

<210> 18
<211> 342
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon US4 from the Fragile Mental
      Retardation-1 (FMR-1) locus 3904 base pairs
      upstream of the 5' end of the CCG repeat region

<400> 18
ctgctgacac tgtaatggtg gatatgttat gcttttttca aaacccacag agctatgcaa 60
cacaaagagt gaatcttaat gtaaactata gactttagtt agtaataatg catcagttgt 120
aataaatgta caatgcgaat gcaagatgtt aataacagga ggaactgtag gggaggaggg 180
agattaggaa gtacatagaa actctctcca ctatctgctt aatttttcca taaatgtaaa 240
actgttctaa aaaataaagc caattaatta ttctttttct aatatctcat ttgaatgaga 300
ggtcatggtt aaaggaaatt gtcagagaaa gtgtcaagaa ag                    342

<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US5
      approximately 4 Kb upstream of the CCG repeat
      region

<400> 19
gacaagccac tgtctgggag                                             20

<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a Fragile Mental
      Retardation-1 (FMR-1) locus region US5
      approximately 4 Kb upstream of the CCG repeat
      region

<400> 20
gacatatggc attgggcatc                                             20

<210> 21
<211> 191
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon US5 from the Fragile Mental
      Retardation-1 (FMR-1) locus 4886 base pairs
      upstream of the 5' end of the CCG repeat region

<400> 21
gacaagccac tgtctgggag aaaatatctg caaaatacat aatctgataa agggctgata 60
tctaaaatat ataaagaaca ctgaaaaatc atcaataaaa aaaacccaat gggtaacaga 120
tctgagcaga caccttatca aagaagatac atacgtgaca aacaagcata agatgcccaa 180
tgccatatgt c                                                      191

<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a 165 base pair
      amplicon upstream of the dystrophia myotonica
      protein kinase (DMPK) gene CTG repeat region

<400> 22
gccaatgacg agttcggacg                                             20

<210> 23
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplifying a 165 base pair
      amplicon upstream of the dystrophia myotonica
      protein kinase (DMPK) gene CTG repeat region

<400> 23
gaggtcaaca cccggcatg                                              19

<210> 24
<211> 165
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon DMPK A1 from the dystrophia
      myotonica protein kinase (DMPK) locus 391 base
      pairs upstream of the 5' end of the CCG repeat
      region

<400> 24
gccaatgacg agttcggacg ggattcgagg cgtgcgagtg gactaacaac agctgtaggc 60
tgttggggcg ggggcggggc gcagggaaga gtgcgggccc acctatgggc gtaggcgggg 120
cgagtcccag gagccaatca gaggcccatg ccgggtgttg acctc                 165

<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplify a 319 base pair
      amplicon (FXN A1) of the frataxin (Friedrich's
      ataxia) locus that lies upstream of the GAA repeat
      region

<400> 25
gaatggctgt ggggatgagg                                             20

<210> 26
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer for amplify a 319 base pair
      amplicon (FXN A1) of the frataxin (Friedrich's
      ataxia) locus that lies upstream of the GAA repeat
      region

<400> 26
cttttaagca ctggcaacca atc                                         23

<210> 27
<211> 319
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic amplicon FXN A1 from the frataxin
      (Friedrich's ataxia) locus 170 base pairs upstream
      of the 5' end of the GAA repeat region

<400> 27
gaatggctgt ggggatgagg aagattcctc aaggggagga catggtattt aatgagggtc 60
ttgaagatgc caaggaagtg gtagagggtg tttcacgagg agggaaccgt ctgggcaaag 120
gccaggaagg cggaagggga tcccttcaga gtggctggta cgccgcatgt attaggggag 180
atgaaagagg caggccacgt ccaagccata tttgtgttgc tctccggagt ttgtacttta 240
ggcttgaact tcccacacgt gttatttggc ccacattgtg tttgaagaaa ctttgggatt 300
ggttgccagt gcttaaaag                                              319


