                         SEQUENCE LISTING

<110>  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
 
<120>  ANALYTICAL METHODS FOR CELL FREE NUCLEIC ACIDS AND APPLICATIONS

<130>  359182D28634

<150>  EP 10305952.3
<151>  2010-09-03

<150>  US 61/380,084
<151>  2010-09-03

<160>  256   

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Kras  rev primer

<400>  1
ggtccttttc cgtgtgtagg                                                   20


<210>  2
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  Kras 409 primer

<400>  2
gcctcccttt ccagcact                                                     18


<210>  3
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Kras 357 primer

<400>  3
ggcatctcta ggacgaaggt                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Kras 300 primer

<400>  4
ggtccttttc cgtgtgtagg                                                   20


<210>  5
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras 249 primer

<400>  5
tggaagagca taggaaagtg c                                                 21


<210>  6
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  Kras 185 primer

<400>  6
atctgcctcc gagttcctg                                                    19


<210>  7
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras 101 primer

<400>  7
ccctacacgt agagcctgtg a                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras  73 primer

<400>  8
gcctgtgacc tacagtgaaa a                                                 21


<210>  9
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras B1 conv G12V primer

<400>  9
acttgtggta gttggagctg t                                                 21


<210>  10
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Kras B2 conv primer

<400>  10
gaatggtcct gcaccagtaa                                                   20


<210>  11
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras conv B1 primer

<400>  11
acttgtggta gttggagctg g                                                 21


<210>  12
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  Kras Conv B2 primer

<400>  12
gattctgaat tagctgtatc gtcaagg                                           27


<210>  13
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Kras Conv A1 primer

<400>  13
taagcgtcga tggaggagtt                                                   20


<210>  14
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras Conv A2 primer

<400>  14
ccagttgact gcagacgtgt a                                                 21


<210>  15
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv MutG12V primer

<400>  15
aactcttgcc tacgccac                                                     18


<210>  16
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv A1 primer

<400>  16
ggagagcagg ataccacagc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv A2 primer

<400>  17
agcaagcaat caaaaactcc                                                   20


<210>  18
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  Braf inv A1 primer

<400>  18
tcatgaagac ctcacagtaa aaa                                               23


<210>  19
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  Braf inv A2 V600E primer

<400>  19
cactccatcg agatttct                                                     18


<210>  20
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  Braf inv B1 primer

<400>  20
ctagaaagtc attgaaggtc tcaac                                             25


<210>  21
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  Braf inv B2 primer

<400>  21
tgatttttgt gaatactggg aac                                               23


<210>  22
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  Braf inv B1 V600E primer

<400>  22
ccatcgagat ttcactgtag ct                                                22


<210>  23
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv A1v1 primer

<400>  23
ttattgactc taagaggaaa gatgaa                                            26


<210>  24
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv A2v2 primer

<400>  24
gagcaagcat tatgaagagt ttagg                                             25


<210>  25
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv B2 primer

<400>  25
tagcctcaat tcttaccatc caca                                              24


<210>  26
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv B1 V600E primer

<400>  26
gattttggtc tagctacaga                                                   20


<210>  27
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Braf conv V600E primer

<400>  27
gctacagtga aatctcgatg g                                                 21


<210>  28
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A1 6126 primer

<400>  28
gcctgctgaa aatgactga                                                    19


<210>  29
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv B1 6379 primer

<400>  29
ccttgggttt caagttatat g                                                 21


<210>  30
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv B2 6445 primer

<400>  30
ccctgacata ctcccaagga                                                   20


<210>  31
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A2 MutG12V primer

<400>  31
ctcttgccta cgccaa                                                       16


<210>  32
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A2 MutG13D64 primer

<400>  32
gcactcttgc ctacgt                                                       16


<210>  33
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A2 G12D low primer

<400>  33
ctcttgccta cgccat                                                       16


<210>  34
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A2 G12A low primer

<400>  34
ctcttgccta cgccag                                                       16


<210>  35
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A2 G12S low primer

<400>  35
tcttgcctac gccact                                                       16


<210>  36
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv A2 G12C low primer

<400>  36
tcttgcctac gccaca                                                       16


<210>  37
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  Kras Inv mut Blocker

<400>  37
gcctacgcca ccagctc                                                      17


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonlucleotides for B2 primers for BRAF conv : B1 
       primer containing the mutation region

<400>  38
gattttggtc tagctacag                                                    19


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  39
atccacaaaa tggatccaga ca                                                22


<210>  40
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  40
attcttacca tccacaaaat gg                                                22


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  41
agcctcaatt cttaccatcc a                                                 21


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  42
gcctcaattc ttaccatcca                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  43
gcctcaattc ttaccatcca                                                   20


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  44
gcctcaattc ttaccatcca c                                                 21


<210>  45
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  45
agcctcaatt cttaccatcc ac                                                22


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  46
cctcaattct taccatccac aa                                                22


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  47
tcagtggaaa aatagcctca a                                                 21


<210>  48
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  48
cagtggaaaa atagcctcaa ttc                                               23


<210>  49
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  49
cagtggaaaa atagcctcaa ttct                                              24


<210>  50
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  50
atcagtggaa aaatagcctc aa                                                22


<210>  51
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  51
tcagtggaaa aatagcctca at                                                22


<210>  52
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  52
tttcaacagg gtacacagaa ca                                                22


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  53
cagggtacac agaacatttt gaa                                               23


<210>  54
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  54
gggtacacag aacattttga aca                                               23


<210>  55
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  55
aacagggtac acagaacatt ttga                                              24


<210>  56
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  56
gattttggtc tagctacag                                                    19


<210>  57
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  57
ttcaacaggg tacacagaac attt                                              24


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  58
tgtggatcac acctgcctta                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  59
tggatcacac ctgccttaaa                                                   20


<210>  60
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  60
gattttggtc tagctacag                                                    19


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  61
gtggatcaca cctgccttaa a                                                 21


<210>  62
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  62
gattgcgaaa cagcttctct g                                                 21


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotides for B2 primers for BRAF conv

<400>  63
tcacacctgc cttaaattgc                                                   20


<210>  64
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev : A2 primer 
       containing the mutation region

<400>  64
cactccatcg agatttc                                                      17


<210>  65
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  65
tcatgaagac ctcacagtaa aaata                                             25


<210>  66
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  66
tgttttcctt tacttactac acctca                                            26


<210>  67
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  67
ctgttttcct ttacttacta cacctca                                           27


<210>  68
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  68
tgttttcctt tacttactac acctcag                                           27


<210>  69
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  69
ttttccttta cttactacac ctcaga                                            26


<210>  70
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  70
gttttccttt acttactaca cctcaga                                           27


<210>  71
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  71
ttattgactc taagaggaaa gatgaa                                            26


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  72
gccccaaaaa tcttaaaagc a                                                 21


<210>  73
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  73
aagccccaaa aatcttaaaa gc                                                22


<210>  74
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  74
ttcaagcccc aaaaatctta aa                                                22


<210>  75
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  75
gccccaaaaa tcttaaaagc                                                   20


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  76
agccccaaaa atcttaaaag c                                                 21


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  77
acacatttca agccccaaaa                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  78
aacacatttc aagccccaaa                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  79
cacatttcaa gccccaaaaa                                                   20


<210>  80
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  80
taacacattt caagccccaa a                                                 21


<210>  81
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected oligonucleotide primers for A1 for BRAF rev

<400>  81
cacatttcaa gccccaaaa                                                    19


<210>  82
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration : B1 
       primer

<400>  82
gtatcgtcaa ggcactcttg c                                                 21


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  83
cttaagcgtc gatggaggag                                                   20


<210>  84
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  84
tgattgaatt ttgtaaggta ttttgaa                                           27


<210>  85
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  85
catgattgaa ttttgtaagg tattttg                                           27


<210>  86
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  86
tcatgattga attttgtaag gtattt                                            26


<210>  87
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  87
gattgaattt tgtaaggtat tttgaa                                            26


<210>  88
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  88
acgtctgcag tcaactggaa t                                                 21


<210>  89
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  89
tgcagtcaac tggaattttc at                                                22


<210>  90
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  90
tctgcagtca actggaattt t                                                 21


<210>  91
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  91
gtatcgtcaa ggcactcttg c                                                 21


<210>  92
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  92
cgatacacgt ctgcagtcaa c                                                 21


<210>  93
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  93
acgatacacg tctgcagtca ac                                                22


<210>  94
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  94
tacgatacac gtctgcagtc aa                                                22


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  95
cttaagcgtc gatggaggag                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  96
taagcgtcga tggaggagtt                                                   20


<210>  97
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  97
cgtcgatgga ggagtttgta a                                                 21


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  98
aagcgtcgat ggaggagttt                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  99
tcttaagcgt cgatggagga                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  100
tttcttaagc gtcgatggag                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  101
tttttcttaa gcgtcgatgg                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  102
tttttcttaa gcgtcgatgg                                                   20


<210>  103
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  103
gcatttttct taagcgtcga t                                                 21


<210>  104
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  104
tgtctatgta gcatttatgc atttttc                                           27


<210>  105
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  105
tgtctatgta gcatttatgc attttt                                            26


<210>  106
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  106
ctgtctatgt agcatttatg cattttt                                           27


<210>  107
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  107
tgtctatgta gcatttatgc atttt                                             25


<210>  108
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  108
ctgtctatgt agcatttatg catttt                                            26


<210>  109
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  109
tctggctatt tagatagaac aacttga                                           27


<210>  110
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  110
ctggctattt agatagaaca acttga                                            26


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  111
gcagaacagc agtctggcta                                                   20


<210>  112
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  112
ctttagccgc cgcagaac                                                     18


<210>  113
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  113
cagaacagca gtctggctat tta                                               23


<210>  114
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  114
gaacagcagt ctggctattt aga                                               23


<210>  115
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  115
agaacagcag tctggctatt taga                                              24


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  116
tcctttgaga gcctttagcc                                                   20


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  117
atcctttgag agcctttagc                                                   20


<210>  118
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  118
tgatcctttg agagccttta gc                                                22


<210>  119
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  119
gtatcgtcaa ggcactcttg c                                                 21


<210>  120
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA oligonucleotides for A1A2 primer pair for detecting 
       KRAS exon 2 hot spot mutations upon conv configuration

<400>  120
atgatccttt gagagccttt agc                                               23


<210>  121
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA region target for B2 primer for detecting KRAS exon 
       2 hot spot mutations upon conv configuration : B1 primer

<400>  121
acttgtggta gttggagctg g                                                 21


<210>  122
<211>  300
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Kras Exon 2

<400>  122
tagtgtatta accttatgtg tgacatgttc taatatagtc acattttcat tatttttatt       60

ataaggcctg ctgaaaatga ctgaatataa acttgtggta gttggagctg gtggcgtagg      120

caagagtgcc ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac      180

aatagaggta aatcttgttt taatatgcat attactggtg caggaccatt ctttgataca      240

gataaaggtt tctctgacca ttttcatgag tacttattac aagataatta tgctgaaagt      300


<210>  123
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo53

<400>  123
gcaagagtgc cttgacgata c                                                 21


<210>  124
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo54

<400>  124
agagtgcctt gacgatacag c                                                 21


<210>  125
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo55

<400>  125
gagtgccttg acgatacagc t                                                 21


<210>  126
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo56

<400>  126
gagtgccttg acgatacagc                                                   20


<210>  127
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo57

<400>  127
agagtgcctt gacgatacag ct                                                22


<210>  128
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo58

<400>  128
ccttgacgat acagctaatt caga                                              24


<210>  129
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo59

<400>  129
ccttgacgat acagctaatt cag                                               23


<210>  130
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo60

<400>  130
ccttgacgat acagctaatt ca                                                22


<210>  131
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo61

<400>  131
tgacgataca gctaattcag aatca                                             25


<210>  132
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo62

<400>  132
ccttgacgat acagctaatt cagaa                                             25


<210>  133
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo63

<400>  133
gctaattcag aatcattttg tgga                                              24


<210>  134
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo64

<400>  134
gctaattcag aatcattttg tgg                                               23


<210>  135
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo65

<400>  135
gctaattcag aatcattttg tggac                                             25


<210>  136
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo66

<400>  136
cagctaattc agaatcattt tgtg                                              24


<210>  137
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo67

<400>  137
cagctaattc agaatcattt tgtgg                                             25


<210>  138
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo73

<400>  138
gaatcatttt gtggacgaat atga                                              24


<210>  139
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo74

<400>  139
gaatcatttt gtggacgaat atg                                               23


<210>  140
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo68

<400>  140
ttgtggacga atatgatcca a                                                 21


<210>  141
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo69

<400>  141
ttttgtggac gaatatgatc ca                                                22


<210>  142
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo70

<400>  142
ttgtggacga atatgatcca ac                                                22


<210>  143
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo71

<400>  143
tgtggacgaa tatgatccaa                                                   20


<210>  144
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo72

<400>  144
ttgtggacga atatgatcca                                                   20


<210>  145
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo75

<400>  145
gtggacgaat atgatccaac aa                                                22


<210>  146
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo76

<400>  146
ttgtggacga atatgatcca ac                                                22


<210>  147
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo77

<400>  147
tgtggacgaa tatgatccaa c                                                 21


<210>  148
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo78

<400>  148
gtggacgaat atgatccaac a                                                 21


<210>  149
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo79

<400>  149
gtggacgaat atgatccaac aat                                               23


<210>  150
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo80

<400>  150
gacgaatatg atccaacaat agagg                                             25


<210>  151
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo81

<400>  151
cgaatatgat ccaacaatag agg                                               23


<210>  152
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo82

<400>  152
acgaatatga tccaacaata gagg                                              24


<210>  153
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo83

<400>  153
cgaatatgat ccaacaatag aggt                                              24


<210>  154
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo84

<400>  154
acgaatatga tccaacaata gaggt                                             25


<210>  155
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo85

<400>  155
tgatccaaca atagaggtaa atcttg                                            26


<210>  156
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo86

<400>  156
cgaatatgat ccaacaatag aggtaa                                            26


<210>  157
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo87

<400>  157
cgaatatgat ccaacaatag aggta                                             25


<210>  158
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo88

<400>  158
acgaatatga tccaacaata gaggta                                            26


<210>  159
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo89

<400>  159
atgatccaac aatagaggta aatcttg                                           27


<210>  160
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA region target for A1 primer for detecting KRAS exon 
       2 hot spot mutations upon rev configuration : A2 primer

<400>  160
gtggcgtagg caagagtgcc tt                                                22


<210>  161
<211>  360
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KRAS exon 2

<400>  161
ttgaaataat ttttcatata aaggtgagtt tgtattaaaa ggtactggtg gagtatttga       60

tagtgtatta accttatgtg tgacatgttc taatatagtc acattttcat tatttttatt      120

ataaggcctg ctgaaaatga ctgaatataa acttgtggta gttggagctg gtggcgtagg      180

caagagtgcc ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac      240

aatagaggta aatcttgttt taatatgcat attactggtg caggaccatt ctttgataca      300

gataaaggtt tctctgacca ttttcatgag tacttattac aagataatta tgctgaaagt      360


<210>  162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo1

<400>  162
aggcctgctg aaaatgactg                                                   20


<210>  163
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo10

<400>  163
tgaatataaa cttgtggtag ttgga                                             25


<210>  164
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo11

<400>  164
ctgaatataa acttgtggta gttgga                                            26


<210>  165
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo12

<400>  165
tgaatataaa cttgtggtag ttggag                                            26


<210>  166
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo13

<400>  166
ctgaatataa acttgtggta gttggag                                           27


<210>  167
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo14

<400>  167
actgaatata aacttgtggt agttgga                                           27


<210>  168
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo15

<400>  168
gaaaatgact gaatataaac ttgtgg                                            26


<210>  169
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo16

<400>  169
gaaaatgact gaatataaac ttgtggt                                           27


<210>  170
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo17

<400>  170
tgactgaata taaacttgtg g                                                 21


<210>  171
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo18

<400>  171
aaaatgactg aatataaact tgtgg                                             25


<210>  172
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo19

<400>  172
aaaatgactg aatataaact tgtggt                                            26


<210>  173
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo20

<400>  173
tgaaaatgac tgaatataaa cttgtgg                                           27


<210>  174
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo21

<400>  174
ctgctgaaaa tgactgaata taaa                                              24


<210>  175
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo22

<400>  175
tgctgaaaat gactgaatat aaa                                               23


<210>  176
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo23

<400>  176
gaaaatgact gaatataaac ttgtgg                                            26


<210>  177
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo24

<400>  177
ctgctgaaaa tgactgaata taaac                                             25


<210>  178
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo2

<400>  178
gcctgctgaa aatgactgaa                                                   20


<210>  179
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo3

<400>  179
aaggcctgct gaaaatgact                                                   20


<210>  180
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo4

<400>  180
ggcctgctga aaatgactga                                                   20


<210>  181
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo5

<400>  181
ggcctgctga aaatgactg                                                    19


<210>  182
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo6

<400>  182
taaggcctgc tgaaaatgac t                                                 21


<210>  183
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo7

<400>  183
taaggcctgc tgaaaatgac                                                   20


<210>  184
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo8

<400>  184
ataaggcctg ctgaaaatga                                                   20


<210>  185
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo9

<400>  185
ataaggcctg ctgaaaatga c                                                 21


<210>  186
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo25

<400>  186
attataaggc ctgctgaaaa tga                                               23


<210>  187
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo26

<400>  187
attataaggc ctgctgaaaa tgac                                              24


<210>  188
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo27

<400>  188
attataaggc ctgctgaaaa tg                                                22


<210>  189
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo28

<400>  189
tattataagg cctgctgaaa atga                                              24


<210>  190
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo29

<400>  190
attataaggc ctgctgaaaa                                                   20


<210>  191
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo30

<400>  191
tttttattat aaggcctgct g                                                 21


<210>  192
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo31

<400>  192
cattattttt attataaggc ctgctg                                            26


<210>  193
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo32

<400>  193
atttttatta taaggcctgc tg                                                22


<210>  194
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo33

<400>  194
ttatttttat tataaggcct gctg                                              24


<210>  195
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo34

<400>  195
tatttttatt ataaggcctg ctg                                               23


<210>  196
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo35

<400>  196
tcattatttt tattataagg cctgctg                                           27


<210>  197
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Selected DNA region target for B1B2 primer pair design for 
       detecting exon 2 KRAS hot spot mutations upon the rev 
       configuration of our method : A1 primer

<400>  197
gcctgctgaa aatgactga                                                    19


<210>  198
<211>  780
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Kras exon 2

<400>  198
tagtgtatta accttatgtg tgacatgttc taatatagtc acattttcat tatttttatt       60

ataaggcctg ctgaaaatga ctgaatataa acttgtggta gttggagctg gtggcgtagg      120

caagagtgcc ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac      180

aatagaggta aatcttgttt taatatgcat attactggtg caggaccatt ctttgataca      240

gataaaggtt tctctgacca ttttcatgag tacttattac aagataatta tgctgaaagt      300

taagttatct gaaatgtacc ttgggtttca agttatatgt aaccattaat atgggaactt      360

tactttcctt gggagtatgt cagggtccat gatgttcact ctctgtgcat tttgattgga      420

agtgtatttc agagtttcgt gagagggtag aaatttgtat cctatctgga cctaaaagac      480

aatcttttta ttgtaacttt tatttttatg ggtttcttgg tattgtgaca tcatatgtaa      540

aggttagatt taattgtact agtgaaatat aattgtttga tggttgattt ttttaaactt      600

catcagcagt attttcctat cttcttctca acattagaga acctacaact accggataaa      660

ttttacaaaa tgaattattt gcctaaggtg tggtttatat aaaggtacta ttaccaactt      720

tacctttgct ttgttgtcat ttttaaattt actcaaggaa atactaggat ttaaaaaaaa      780


<210>  199
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo42

<400>  199
tgaaatgtac cttgggtttc aa                                                22


<210>  200
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo43

<400>  200
tgaaatgtac cttgggtttc a                                                 21


<210>  201
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo44

<400>  201
tgaaatgtac cttgggtttc aag                                               23


<210>  202
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo45

<400>  202
gaaatgtacc ttgggtttca agtt                                              24


<210>  203
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo46

<400>  203
gaaatgtacc ttgggtttca ag                                                22


<210>  204
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo37

<400>  204
tccttgggag tatgtcaggg                                                   20


<210>  205
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo38

<400>  205
tgtcagggtc catgatgttc                                                   20


<210>  206
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo39

<400>  206
ccttgggagt atgtcagggt                                                   20


<210>  207
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo40

<400>  207
ggagtatgtc agggtccatg a                                                 21


<210>  208
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo41

<400>  208
tgtgcatttt gattggaagt g                                                 21


<210>  209
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo47

<400>  209
cttgggagta tgtcagggtc c                                                 21


<210>  210
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo48

<400>  210
tccttgggag tatgtcaggg t                                                 21


<210>  211
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo49

<400>  211
tgtcagggtc catgatgttc                                                   20


<210>  212
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo51

<400>  212
tctctgtgca ttttgattgg a                                                 21


<210>  213
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo50

<400>  213
tttcagagtt tcgtgagagg g                                                 21


<210>  214
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Amo52

<400>  214
ttcagagttt cgtgagaggg                                                   20


<210>  215
<211>  1320
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  KRAS exon 2

<400>  215
ttatttcccc atgacacaat ccagctttat ttgacactca ttctctcaac tctcatctga       60

ttcttactgt taatatttat ccaagagaac tactgccatg atgctttaaa agtttttctg      120

tagctgttgc atattgactt ctaacactta gaggtggggg tccactagga aaactgtaac      180

aataagagtg gagatagctg tcagcaactt ttgtgagggt gtgctacagg gtgtagagca      240

ctgtgaagtc tctacatgag tgaagtcatg atatgatcct ttgagagcct ttagccgccg      300

cagaacagca gtctggctat ttagatagaa caacttgatt ttaagataaa agaactgtct      360

atgtagcatt tatgcatttt tcttaagcgt cgatggagga gtttgtaaat gaagtacagt      420

tcattacgat acacgtctgc agtcaactgg aattttcatg attgaatttt gtaaggtatt      480

ttgaaataat ttttcatata aaggtgagtt tgtattaaaa ggtactggtg gagtatttga      540

tagtgtatta accttatgtg tgacatgttc taatatagtc acattttcat tatttttatt      600

ataaggcctg ctgaaaatga ctgaatataa acttgtggta gttggagctg gtggcgtagg      660

caagagtgcc ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac      720

aatagaggta aatcttgttt taatatgcat attactggtg caggaccatt ctttgataca      780

gataaaggtt tctctgacca ttttcatgag tacttattac aagataatta tgctgaaagt      840

taagttatct gaaatgtacc ttgggtttca agttatatgt aaccattaat atgggaactt      900

tactttcctt gggagtatgt cagggtccat gatgttcact ctctgtgcat tttgattgga      960

agtgtatttc agagtttcgt gagagggtag aaatttgtat cctatctgga cctaaaagac     1020

aatcttttta ttgtaacttt tatttttatg ggtttcttgg tattgtgaca tcatatgtaa     1080

aggttagatt taattgtact agtgaaatat aattgtttga tggttgattt ttttaaactt     1140

catcagcagt attttcctat cttcttctca acattagaga acctacaact accggataaa     1200

ttttacaaaa tgaattattt gcctaaggtg tggtttatat aaaggtacta ttaccaactt     1260

tacctttgct ttgttgtcat ttttaaattt actcaaggaa atactaggat ttaaaaaaaa     1320


<210>  216
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for mutated amplicon

<400>  216
tggtagttgg agctggtggc gtaggcaaga gtgccttgac gatacagcta attcagaatc       60

attttgtgga cgaatatgat c                                                 81


<210>  217
<211>  331
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pair for non mutated amplicon

<400>  217
tcagcaactt ttgtgagggt gtgctacagg gtgtagagca ctgtgaagtc tctacatgag       60

tgaagtcatg atatgatcct ttgagagcct ttagccgccg cagaacagca gtctggctat      120

ttagatagaa caacttgatt ttaagataaa agaactgtct atgtagcatt tatgcatttt      180

tcttaagcgt cgatggagga gtttgtaaat gaagtacagt tcattacgat acacgtctgc      240

agtcaactgg aattttcatg attgaatttt gtaaggtatt ttgaaataat ttttcatata      300

aaggtgagtt tgtattaaaa ggtactggtg g                                     331


<210>  218
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for mutated amplicon

<400>  218
tattaacctt atgtgtgaca tgttctaata tagtcacatt ttcattattt ttattataag       60

gcctgctgaa aatga                                                        75


<210>  219
<211>  332
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pair for non mutated amplicon

<400>  219
aatcttgttt taatatgcat attactggtg caggaccatt ctttgataca gataaaggtt       60

tctctgacca ttttcatgag tacttattac aagataatta tgctgaaagt taagttatct      120

gaaatgtacc ttgggtttca agttatatgt aaccattaat atgggaactt tactttcctt      180

gggagtatgt cagggtccat gatgttcact ctctgtgcat tttgattgga agtgtatttc      240

agagtttcgt gagagggtag aaatttgtat cctatctgga cctaaaagac aatcttttta      300

ttgtaacttt tatttttatg ggtttcttgg ta                                    332


<210>  220
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 60 Hf primer

<400>  220
gcctgtgacc tacagtgaaa a                                                 21


<210>  221
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 73 Hf primer

<400>  221
ccctacacgt agagcctgtg a                                                 21


<210>  222
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 101 Hf primer

<400>  222
gagatggtgg aagaacaggt g                                                 21


<210>  223
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 145 Hf primer

<400>  223
tgggctgtga cattgctg                                                     18


<210>  224
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 185 Hf primer

<400>  224
atctgcctcc gagttcctg                                                    19


<210>  225
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 249 Hf primer

<400>  225
tggaagagca taggaaagtg c                                                 21


<210>  226
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 300 Hf primer

<400>  226
ggtccttttc cgtgtgtagg                                                   20


<210>  227
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 357 Hf

<400>  227
ggcatctcta ggacgaaggt                                                   20


<210>  228
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 409 Hf

<400>  228
gcctcccttt ccagcact                                                     18


<210>  229
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 145-300r primer

<400>  229
tgaccaagca aaacagacca                                                   20


<210>  230
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 46 Hr primer

<400>  230
gctgtatcgt caaggcactc                                                   20


<210>  231
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 82 Hr primer

<400>  231
ttggatcata ttcgtccaca a                                                 21


<210>  232
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 138 Hr primer

<400>  232
caaagaatgg tcctgcacc                                                    19


<210>  233
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 200 Hr primer

<400>  233
tgaaaatggt cagagaaacc tt                                                22


<210>  234
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 250 Hr primer

<400>  234
tgaaacccaa ggtacatttc ag                                                22


<210>  235
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 300 Hr primer

<400>  235
gaacatcatg gaccctgaca                                                   20


<210>  236
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 350 Hr primer

<400>  236
ttctaccctc tcacgaaact ctg                                               23


<210>  237
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras 400 Hr primer

<400>  237
aaagattgtc ttttaggtcc agatagg                                           27


<210>  238
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KrasNonMutated Hf primer

<400>  238
gtagttggag ctggtggc                                                     18


<210>  239
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras G13D Hf primer

<400>  239
gtagttggag ctggtga                                                      17


<210>  240
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras G12V Hf primer

<400>  240
ttgtggtagt tggagctgt                                                    19


<210>  241
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras G12D Hf primer

<400>  241
tgtggtagtt ggagctga                                                     18


<210>  242
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras G12S Hf primer

<400>  242
acttgtggta gttggagcta                                                   20


<210>  243
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Kras G12A Hf primer

<400>  243
tgtggtagtt ggagctgc                                                     18


<210>  244
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12V

<400>  244
ttgtggtagt tggagctgt                                                    19


<210>  245
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG13D

<400>  245
gtagttggag ctggtga                                                      17


<210>  246
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12D

<400>  246
tgtggtagtt ggagctga                                                     18


<210>  247
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12S

<400>  247
acttgtggta gttggagcta                                                   20


<210>  248
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12A

<400>  248
tgtggtagtt ggagctgc                                                     18


<210>  249
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12C

<400>  249
acttgtggta gttggagctt                                                   20


<210>  250
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A2

<400>  250
aaggcactct tgcctacgcc ac                                                22


<210>  251
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12V

<400>  251
aaggcactct tgcctacgcc at                                                22


<210>  252
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG13D

<400>  252
aaggcactct tgcctacgcc aa                                                22


<210>  253
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12D

<400>  253
aaggcactct tgcctacgcc aa                                                22


<210>  254
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12S

<400>  254
aaggcactct tgcctacgcc aa                                                22


<210>  255
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12A

<400>  255
aaggcactct tgcctacgcc ac                                                22


<210>  256
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer for mutation KrasG12C

<400>  256
aaggcactct tgcctacgcc at                                                22


