                         SEQUENCE LISTING

<110>  E.I. duPont de Nemours & Co., Inc.
       Zhu, Quinn
 
<120>  FURTHER IMPROVED OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR 
       HIGH EICOSAPENTAENOIC ACID PRODUCTION

<130>  CL5411

<150>  US61/479921
<151>  2011-04-28

<160>  435   

<170>  PatentIn version 3.5

<210>  1
<211>  258
<212>  PRT
<213>  Euglena gracilis


<220>
<221>  MISC_FEATURE
<222>  (1)..(258)
<223>  mutant delta-9 elongase; EgD9eS-mutant consensus

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa = Ser [S] (synthetic codon-optimized) or (Ala [A] or Asp [D] 
       or Gly [G] or Ile [I] or Lys [K] or Gln [Q]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa =  Gln [Q] (synthetic codon-optimized) or Lys [K] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa =  Ala [A] (synthetic codon-optimized) or (Asp [D] or Thr [T]
       or Val [V]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa =  Val [V] (synthetic codon-optimized) or Phe [F] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or (Phe [F] or Gly [G]
       or Met [M]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (58)..(58)
<223>  Xaa =  Lys [K] (synthetic codon-optimized) or Arg [R] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa =  Tyr [Y] (synthetic codon-optimized) or Cys [C] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (98)..(98)
<223>  Xaa =  Asp [D] (synthetic codon-optimized) or Gly [G] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (107)..(107)
<223>  Xaa =  Gln [Q] (synthetic codon-optimized) or Glu [E] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (108)..(108)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or Gly [G] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (127)..(127)
<223>  Xaa =  Gly [G] (synthetic codon-optimized) or Leu [L] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (130)..(130)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or Met [M] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  Xaa =  Trp [W] (synthetic codon-optimized) or Thr [T] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (143)..(143)
<223>  Xaa =  Met [M] (synthetic codon-optimized) or (Asn [N] or Trp 
       [W]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (161)..(161)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or (Thr [T] or Tyr 
       [Y]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (168)..(168)
<223>  Xaa =  Trp [W] (synthetic codon-optimized) or Gly [G] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (179)..(179)
<223>  Xaa =  Ile [I] (synthetic codon-optimized) or (Met [M] or Arg 
       [R]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (236)..(236)
<223>  Xaa =  Cys [C] (synthetic codon-optimized) or Asn [N] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (243)..(243)
<223>  Xaa =  Val [V] (synthetic codon-optimized) or Ala [A] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (244)..(244)
<223>  Xaa =  Gln [Q] (synthetic codon-optimized) or Asn [N] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (254)..(254)
<223>  Xaa =  Ala [A] (synthetic codon-optimized) or (Trp [W] or Tyr 
       [Y]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (257)..(257)
<223>  Xaa =  Ile [I] (synthetic codon-optimized) or Thr [T] (mutant)

<400>  1

Met Glu Val Val Asn Glu Ile Val Xaa Ile Gly Xaa Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Xaa Gln Leu Trp Ser Asp Ala Ser His Cys Glu Xaa 
            20                  25                  30          


Leu Tyr Xaa Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Xaa Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Xaa Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Xaa Asn Asn Val Phe Arg Ile Thr Thr Xaa Xaa Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Xaa Lys 
        115                 120                 125             


Pro Xaa Thr Xaa Leu Gln Phe Phe His His Leu Gly Ala Pro Xaa Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Xaa Leu Asn Gly Phe Ile His Xaa Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Xaa Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Xaa Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Xaa Xaa Thr Tyr Ile Val Arg Lys His Lys Gly Xaa Lys Lys 
                245                 250                 255     


Xaa Gln 
        


<210>  2
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(777)

<400>  2
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  3
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  3

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  4
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(24)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(24)

<400>  4

Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr 
1               5                   10                  15      


Asp Ser Leu Ala Val Met Gly Ser 
            20                  


<210>  5
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(19)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(19)

<400>  5

Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr Asp Ser 
1               5                   10                  15      


Leu Ala Val 
            


<210>  6
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<400>  6

Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu 
1               5                   10                  15      


Ala Val 
        


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(18)

<400>  7

Pro Thr Arg Pro Ala Gly Pro Pro Pro Ala Thr Tyr Tyr Asp Ser Leu 
1               5                   10                  15      


Ala Val 
        


<210>  8
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(33)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(33)

<400>  8

Pro Gly Gly Pro Gly Lys Pro Ser Glu Ile Ala Ser Leu Pro Pro Pro 
1               5                   10                  15      


Ile Arg Pro Val Gly Asn Pro Pro Ala Ala Tyr Tyr Asp Ala Leu Ala 
            20                  25                  30          


Thr 
    


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(22)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(22)

<400>  9

Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu 
1               5                   10                  15      


Ala Val Ser Gly Arg Thr 
            20          


<210>  10
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Multizyme linker

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(36)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(36)

<400>  10

Pro Gly Gly Pro Gly Lys Pro Ser Glu Ile Ala Ser Leu Pro Pro Pro 
1               5                   10                  15      


Ile Arg Pro Val Gly Asn Pro Pro Ala Ala Tyr Tyr Asp Ala Leu Ala 
            20                  25                  30          


Thr Gly Arg Thr 
        35      


<210>  11
<211>  2112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multizyme: EgD9ES/EgD8M gene fusion


<220>
<221>  CDS
<222>  (1)..(2112)
<223>  Multizyme: EgD9ES/EgD8M gene fusion

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2112)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2112)

<400>  11
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag ggc gcc ggt ccc gct cga cct gcc gga ctt cct ccc gct acc        816
Ile Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr           
            260                 265                 270                   

tac tac gac tct ctg gcc gtc atg gga tcc gtg aag gct tct cga cag        864
Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val Lys Ala Ser Arg Gln           
        275                 280                 285                       

gct ctg ccc ctc gtc atc gac gga aag gtg tac gac gtc tcc gct tgg        912
Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr Asp Val Ser Ala Trp           
    290                 295                 300                           

gtg aac ttc cac cct ggt gga gct gaa atc att gag aac tac cag gga        960
Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile Glu Asn Tyr Gln Gly           
305                 310                 315                 320           

cga gat gct act gac gcc ttc atg gtt atg cac tct cag gaa gcc ttc       1008
Arg Asp Ala Thr Asp Ala Phe Met Val Met His Ser Gln Glu Ala Phe           
                325                 330                 335               

gac aag ctc aag cga atg ccc aag atc aac cag gct tcc gag ctg cct       1056
Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln Ala Ser Glu Leu Pro           
            340                 345                 350                   

ccc cag gct gcc gtc aac gaa gct cag gag gat ttc cga aag ctc cga       1104
Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp Phe Arg Lys Leu Arg           
        355                 360                 365                       

gaa gag ctg atc gcc act ggc atg ttt gac gcc tct ccc ctc tgg tac       1152
Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala Ser Pro Leu Trp Tyr           
    370                 375                 380                           

tcg tac aag atc ttg acc acc ctg ggt ctt ggc gtg ctt gcc ttc ttc       1200
Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly Val Leu Ala Phe Phe           
385                 390                 395                 400           

atg ctg gtc cag tac cac ctg tac ttc att ggt gct ctc gtg ctc ggt       1248
Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly Ala Leu Val Leu Gly           
                405                 410                 415               

atg cac tac cag caa atg gga tgg ctg tct cat gac atc tgc cac cac       1296
Met His Tyr Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys His His           
            420                 425                 430                   

cag acc ttc aag aac cga aac tgg aat aac gtc ctg ggt ctg gtc ttt       1344
Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val Leu Gly Leu Val Phe           
        435                 440                 445                       

ggc aac gga ctc cag ggc ttc tcc gtg acc tgg tgg aag gac aga cac       1392
Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp Arg His           
    450                 455                 460                           

aac gcc cat cat tct gct acc aac gtt cag ggt cac gat ccc gac att       1440
Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro Asp Ile           
465                 470                 475                 480           

gat aac ctg cct ctg ctc gcc tgg tcc gag gac gat gtc act cga gct       1488
Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp Asp Val Thr Arg Ala           
                485                 490                 495               

tct ccc atc tcc cga aag ctc att cag ttc caa cag tac tat ttc ctg       1536
Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln Gln Tyr Tyr Phe Leu           
            500                 505                 510                   

gtc atc tgt att ctc ctg cga ttc atc tgg tgt ttc cag tct gtg ctg       1584
Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser Val Leu           
        515                 520                 525                       

acc gtt cga tcc ctc aag gac cga gac aac cag ttc tac cga tct cag       1632
Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln Phe Tyr Arg Ser Gln           
    530                 535                 540                           

tac aag aaa gag gcc att gga ctc gct ctg cac tgg act ctc aag acc       1680
Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His Trp Thr Leu Lys Thr           
545                 550                 555                 560           

ctg ttc cac ctc ttc ttt atg ccc tcc atc ctg acc tcg atg ctg gtg       1728
Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu Thr Ser Met Leu Val           
                565                 570                 575               

ttc ttt gtt tcc gag ctc gtc ggt ggc ttc gga att gcc atc gtg gtc       1776
Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly Ile Ala Ile Val Val           
            580                 585                 590                   

ttc atg aac cac tac cct ctg gag aag atc ggt gat tcc gtc tgg gac       1824
Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly Asp Ser Val Trp Asp           
        595                 600                 605                       

gga cat ggc ttc tct gtg ggt cag atc cat gag acc atg aac att cga       1872
Gly His Gly Phe Ser Val Gly Gln Ile His Glu Thr Met Asn Ile Arg           
    610                 615                 620                           

cga ggc atc att act gac tgg ttc ttt gga ggc ctg aac tac cag atc       1920
Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr Gln Ile           
625                 630                 635                 640           

gag cac cat ctc tgg ccc acc ctg cct cga cac aac ctc act gcc gtt       1968
Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr Ala Val           
                645                 650                 655               

tcc tac cag gtg gaa cag ctg tgc cag aag cac aac ctc ccc tac cga       2016
Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His Asn Leu Pro Tyr Arg           
            660                 665                 670                   

aac cct ctg ccc cat gaa ggt ctc gtc atc ctg ctc cga tac ctg tcc       2064
Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu Leu Arg Tyr Leu Ser           
        675                 680                 685                       

cag ttc gct cga atg gcc gag aag cag ccc ggt gcc aag gct cag taa       2112
Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly Ala Lys Ala Gln               
    690                 695                 700                           


<210>  12
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr 
            260                 265                 270         


Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val Lys Ala Ser Arg Gln 
        275                 280                 285             


Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr Asp Val Ser Ala Trp 
    290                 295                 300                 


Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile Glu Asn Tyr Gln Gly 
305                 310                 315                 320 


Arg Asp Ala Thr Asp Ala Phe Met Val Met His Ser Gln Glu Ala Phe 
                325                 330                 335     


Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln Ala Ser Glu Leu Pro 
            340                 345                 350         


Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp Phe Arg Lys Leu Arg 
        355                 360                 365             


Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala Ser Pro Leu Trp Tyr 
    370                 375                 380                 


Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly Val Leu Ala Phe Phe 
385                 390                 395                 400 


Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly Ala Leu Val Leu Gly 
                405                 410                 415     


Met His Tyr Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys His His 
            420                 425                 430         


Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val Leu Gly Leu Val Phe 
        435                 440                 445             


Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp Arg His 
    450                 455                 460                 


Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro Asp Ile 
465                 470                 475                 480 


Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp Asp Val Thr Arg Ala 
                485                 490                 495     


Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln Gln Tyr Tyr Phe Leu 
            500                 505                 510         


Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser Val Leu 
        515                 520                 525             


Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln Phe Tyr Arg Ser Gln 
    530                 535                 540                 


Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His Trp Thr Leu Lys Thr 
545                 550                 555                 560 


Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu Thr Ser Met Leu Val 
                565                 570                 575     


Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly Ile Ala Ile Val Val 
            580                 585                 590         


Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly Asp Ser Val Trp Asp 
        595                 600                 605             


Gly His Gly Phe Ser Val Gly Gln Ile His Glu Thr Met Asn Ile Arg 
    610                 615                 620                 


Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr Gln Ile 
625                 630                 635                 640 


Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr Ala Val 
                645                 650                 655     


Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His Asn Leu Pro Tyr Arg 
            660                 665                 670         


Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu Leu Arg Tyr Leu Ser 
        675                 680                 685             


Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly Ala Lys Ala Gln 
    690                 695                 700             


<210>  13
<211>  2109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multizyme: EaD9ES/EaD8S gene fusion


<220>
<221>  CDS
<222>  (1)..(2109)
<223>  Multizyme: EaD9ES/EaD8S gene fusion

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2109)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2109)

<400>  13
atg gag gct gcc aag gag ctg gtc tcc atc gtc cag gag gaa ctt ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tac gcc cag ctc tgg cag gac gcc tcc tct tgc gag gtt         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcg gtc gct ttc gtg gcc atc aag ttc atg ctt cga cct        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctc aag cga caa gcc acc ctc aaa aag ctg ttc acc gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttt ctc atg tcc atc tac tcg ttc ggc tcc ttc ctg gcg atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tac gct ctc tct gtc act ggt att ctt tcc ggc gat tgt gag act gcc        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aat gac gtg ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gtc gag tac atc gac tcc ttc tac ctt ccc ctc atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

ccc ttg tcg ttt ctg cag ttc ttt cac cat ctc gga gct ccc atc gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aag tat cga aac gaa ggc gtc tgg atc ttt gtt        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctc atc aag ctg aac ttc cct atg ccc aag aac ctc att acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg caa att atc cag ttc aac gtc gga ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cga aac gtg ccc tgc tac cgg cag gac ggt atg cga atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggc acg gtg ctg ctt ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtc cag acc tac att cgg aag cct cga aag aac cga ggc aaa        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag ggc gcc ggt ccc gct cga cct gcc gga ctt cct ccc gct acc        816
Lys Glu Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr           
            260                 265                 270                   

tac tac gac tct ctg gcc gtc atg gga tcc atg gtc aag cga ccc gct        864
Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Met Val Lys Arg Pro Ala           
        275                 280                 285                       

ctg cct ctc acc gtg gac ggt gtc acc tac gac gtt tct gcc tgg ctc        912
Leu Pro Leu Thr Val Asp Gly Val Thr Tyr Asp Val Ser Ala Trp Leu           
    290                 295                 300                           

aac cac cat ccc gga ggt gcc gac att atc gag aac tac cga ggt cgg        960
Asn His His Pro Gly Gly Ala Asp Ile Ile Glu Asn Tyr Arg Gly Arg           
305                 310                 315                 320           

gat gct acc gac gtc ttc atg gtt atg cac tcc gag aac gcc gtg tcc       1008
Asp Ala Thr Asp Val Phe Met Val Met His Ser Glu Asn Ala Val Ser           
                325                 330                 335               

aaa ctc aga cga atg ccc atc atg gaa cct tcc tct ccc ctg act cca       1056
Lys Leu Arg Arg Met Pro Ile Met Glu Pro Ser Ser Pro Leu Thr Pro           
            340                 345                 350                   

aca cct ccc aag cca aac tcc gac gaa cct cag gag gat ttc cga aag       1104
Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro Gln Glu Asp Phe Arg Lys           
        355                 360                 365                       

ctg cga gac gag ctc att gct gca ggc atg ttc gat gcc tct ccc atg       1152
Leu Arg Asp Glu Leu Ile Ala Ala Gly Met Phe Asp Ala Ser Pro Met           
    370                 375                 380                           

tgg tac gct tac aag acc ctg tcg act ctc gga ctg ggt gtc ctt gcc       1200
Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu Gly Leu Gly Val Leu Ala           
385                 390                 395                 400           

gtg ctg ttg atg acc cag tgg cac tgg tac ctg gtt ggt gct atc gtc       1248
Val Leu Leu Met Thr Gln Trp His Trp Tyr Leu Val Gly Ala Ile Val           
                405                 410                 415               

ctc ggc att cac ttt caa cag atg gga tgg ctc tcg cac gac att tgc       1296
Leu Gly Ile His Phe Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys           
            420                 425                 430                   

cat cac cag ctg ttc aag gac cga tcc atc aac aat gcc att ggc ctg       1344
His His Gln Leu Phe Lys Asp Arg Ser Ile Asn Asn Ala Ile Gly Leu           
        435                 440                 445                       

ctc ttc gga aac gtg ctt cag ggc ttt tct gtc act tgg tgg aag gac       1392
Leu Phe Gly Asn Val Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp           
    450                 455                 460                           

cga cac aac gct cat cac tcc gcc acc aac gtg cag ggt cac gat ccc       1440
Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro           
465                 470                 475                 480           

gac atc gac aac ctg cct ctc ctg gcg tgg tcc aag gag gac gtc gag       1488
Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Lys Glu Asp Val Glu           
                485                 490                 495               

cga gct ggc ccg ttt tct cga cgg atg atc aag tac caa cag tat tac       1536
Arg Ala Gly Pro Phe Ser Arg Arg Met Ile Lys Tyr Gln Gln Tyr Tyr           
            500                 505                 510                   

ttc ttt ttc atc tgt gcc ctt ctg cga ttc atc tgg tgc ttt cag tcc       1584
Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser           
        515                 520                 525                       

att cat act gcc acg ggt ctc aag gat cga agc aat cag tac tat cga       1632
Ile His Thr Ala Thr Gly Leu Lys Asp Arg Ser Asn Gln Tyr Tyr Arg           
    530                 535                 540                           

aga cag tac gag aag gag tcc gtc ggt ctg gca ctc cac tgg ggt ctc       1680
Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu Ala Leu His Trp Gly Leu           
545                 550                 555                 560           

aag gcc ttg ttc tac tat ttc tac atg ccc tcg ttt ctc acc gga ctc       1728
Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro Ser Phe Leu Thr Gly Leu           
                565                 570                 575               

atg gtg ttc ttt gtc tcc gag ctg ctt ggt ggc ttc gga att gcc atc       1776
Met Val Phe Phe Val Ser Glu Leu Leu Gly Gly Phe Gly Ile Ala Ile           
            580                 585                 590                   

gtt gtc ttc atg aac cac tac cct ctg gag aag att cag gac tcc gtg       1824
Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gln Asp Ser Val           
        595                 600                 605                       

tgg gat ggt cat ggc ttc tgt gct gga cag att cac gag acc atg aac       1872
Trp Asp Gly His Gly Phe Cys Ala Gly Gln Ile His Glu Thr Met Asn           
    610                 615                 620                           

gtt cag cga ggc ctc gtc aca gac tgg ttt ttc ggt ggc ctc aac tac       1920
Val Gln Arg Gly Leu Val Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr           
625                 630                 635                 640           

cag atc gaa cat cac ctg tgg cct act ctt ccc aga cac aac ctc acc       1968
Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr           
                645                 650                 655               

gct gcc tcc atc aaa gtg gag cag ctg tgc aag aag cac aac ctg ccc       2016
Ala Ala Ser Ile Lys Val Glu Gln Leu Cys Lys Lys His Asn Leu Pro           
            660                 665                 670                   

tac cga tct cct ccc atg ctc gaa ggt gtc ggc att ctt atc tcc tac       2064
Tyr Arg Ser Pro Pro Met Leu Glu Gly Val Gly Ile Leu Ile Ser Tyr           
        675                 680                 685                       

ctg ggc acc ttc gct cga atg gtt gcc aag gca gac aag gcc taa           2109
Leu Gly Thr Phe Ala Arg Met Val Ala Lys Ala Asp Lys Ala                   
    690                 695                 700                           


<210>  14
<211>  702
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu Gly Ala Gly Pro Ala Arg Pro Ala Gly Leu Pro Pro Ala Thr 
            260                 265                 270         


Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Met Val Lys Arg Pro Ala 
        275                 280                 285             


Leu Pro Leu Thr Val Asp Gly Val Thr Tyr Asp Val Ser Ala Trp Leu 
    290                 295                 300                 


Asn His His Pro Gly Gly Ala Asp Ile Ile Glu Asn Tyr Arg Gly Arg 
305                 310                 315                 320 


Asp Ala Thr Asp Val Phe Met Val Met His Ser Glu Asn Ala Val Ser 
                325                 330                 335     


Lys Leu Arg Arg Met Pro Ile Met Glu Pro Ser Ser Pro Leu Thr Pro 
            340                 345                 350         


Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro Gln Glu Asp Phe Arg Lys 
        355                 360                 365             


Leu Arg Asp Glu Leu Ile Ala Ala Gly Met Phe Asp Ala Ser Pro Met 
    370                 375                 380                 


Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu Gly Leu Gly Val Leu Ala 
385                 390                 395                 400 


Val Leu Leu Met Thr Gln Trp His Trp Tyr Leu Val Gly Ala Ile Val 
                405                 410                 415     


Leu Gly Ile His Phe Gln Gln Met Gly Trp Leu Ser His Asp Ile Cys 
            420                 425                 430         


His His Gln Leu Phe Lys Asp Arg Ser Ile Asn Asn Ala Ile Gly Leu 
        435                 440                 445             


Leu Phe Gly Asn Val Leu Gln Gly Phe Ser Val Thr Trp Trp Lys Asp 
    450                 455                 460                 


Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly His Asp Pro 
465                 470                 475                 480 


Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Lys Glu Asp Val Glu 
                485                 490                 495     


Arg Ala Gly Pro Phe Ser Arg Arg Met Ile Lys Tyr Gln Gln Tyr Tyr 
            500                 505                 510         


Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe Ile Trp Cys Phe Gln Ser 
        515                 520                 525             


Ile His Thr Ala Thr Gly Leu Lys Asp Arg Ser Asn Gln Tyr Tyr Arg 
    530                 535                 540                 


Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu Ala Leu His Trp Gly Leu 
545                 550                 555                 560 


Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro Ser Phe Leu Thr Gly Leu 
                565                 570                 575     


Met Val Phe Phe Val Ser Glu Leu Leu Gly Gly Phe Gly Ile Ala Ile 
            580                 585                 590         


Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gln Asp Ser Val 
        595                 600                 605             


Trp Asp Gly His Gly Phe Cys Ala Gly Gln Ile His Glu Thr Met Asn 
    610                 615                 620                 


Val Gln Arg Gly Leu Val Thr Asp Trp Phe Phe Gly Gly Leu Asn Tyr 
625                 630                 635                 640 


Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His Asn Leu Thr 
                645                 650                 655     


Ala Ala Ser Ile Lys Val Glu Gln Leu Cys Lys Lys His Asn Leu Pro 
            660                 665                 670         


Tyr Arg Ser Pro Pro Met Leu Glu Gly Val Gly Ile Leu Ile Ser Tyr 
        675                 680                 685             


Leu Gly Thr Phe Ala Arg Met Val Ala Lys Ala Asp Lys Ala 
    690                 695                 700         


<210>  15
<211>  2127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multizyme: E389D9eS/EgD8M gene fusion


<220>
<221>  CDS
<222>  (1)..(2127)
<223>  Multizyme: E389D9eS/EgD8M gene fusion

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US-2008-0254191-A1
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2127)

<300>
<302>  MULTIZYMES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  WO 2008/124048
<311>  2008-04-03
<312>  2008-10-16
<313>  (1)..(2127)

<400>  15
atg gct gcc gtc atc gag gtg gcc aac gag ttc gtc gct atc act gcc         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gag acc ctt ccc aag gtg gac tat cag cga ctc tgg cga gac atc tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

tcc tgc gag ctc ctg tac ttc tcc att gct ttc gtc atc ctc aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acc ctt ggc gag ctc tcg gat tct ggc aaa aag att ctg cga gtg ctg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aac ctc ttc atg tcc gtc ttt tcg ctg gtg tcc ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctc tgt atg ggt tac gcc atc tac acc gtt gga ctg tac tcc aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac aga gct ttc gac aac agc ttg ttc cga ttt gcc acc aag gtc        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tat tcc aag ttt ctg gag tac atc gac tct ttc tac ctt ccc        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag cct ctg tcc ttt ctg cag ttc ttt cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gct cct atg gac atg tgg ctc ttc gtg cag tac tct ggc gaa tcc att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttc ctg aac gga ttc att cac ttt gtc atg tac ggc        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tat tgg aca cgg ctg atg aag ttc aac ttt ccc atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ctc att acc gca atg cag atc acc cag ttc aac gtt ggc ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aag gac att ccc tgt tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ctg gcc tgg atc ttc aac tac tgg tac gtc ggt acc gtt ctt ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctc ttc atc aac ttc ttt gtc aag tcc tac gtg ttt ccc aag cct aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gcc gac aaa aag gtc cag ggc gcc ggt ccc gct cga cct gcc gga        816
Thr Ala Asp Lys Lys Val Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly           
            260                 265                 270                   

ctt cct ccc gct acc tac tac gac tct ctg gcc gtc atg gga tcc gtg        864
Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val           
        275                 280                 285                       

aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag gtg tac        912
Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr           
    290                 295                 300                           

gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa atc att        960
Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile           
305                 310                 315                 320           

gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt atg cac       1008
Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met His           
                325                 330                 335               

tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc aac cag       1056
Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln           
            340                 345                 350                   

gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag gag gat       1104
Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp           
        355                 360                 365                       

ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt gac gcc       1152
Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala           
    370                 375                 380                           

tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt ctt ggc       1200
Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly           
385                 390                 395                 400           

gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc att ggt       1248
Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly           
                405                 410                 415               

gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg tct cat       1296
Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser His           
            420                 425                 430                   

gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat aac gtc       1344
Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val           
        435                 440                 445                       

ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg acc tgg       1392
Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp           
    450                 455                 460                           

tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt cag ggt       1440
Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly           
465                 470                 475                 480           

cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc gag gac       1488
His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp           
                485                 490                 495               

gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag ttc caa       1536
Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln           
            500                 505                 510                   

cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc tgg tgt       1584
Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys           
        515                 520                 525                       

ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac aac cag       1632
Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln           
    530                 535                 540                           

ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct ctg cac       1680
Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His           
545                 550                 555                 560           

tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc atc ctg       1728
Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu           
                565                 570                 575               

acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc ttc gga       1776
Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly           
            580                 585                 590                   

att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag atc ggt       1824
Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly           
        595                 600                 605                       

gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc cat gag       1872
Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His Glu           
    610                 615                 620                           

acc atg aac att cga cga ggc atc att act gac tgg ttc ttt gga ggc       1920
Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly           
625                 630                 635                 640           

ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct cga cac       1968
Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His           
                645                 650                 655               

aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag aag cac       2016
Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His           
            660                 665                 670                   

aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc atc ctg       2064
Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu           
        675                 680                 685                       

ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag ccc ggt       2112
Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly           
    690                 695                 700                           

gcc aag gct cag taa                                                   2127
Ala Lys Ala Gln                                                           
705                                                                       


<210>  16
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln Gly Ala Gly Pro Ala Arg Pro Ala Gly 
            260                 265                 270         


Leu Pro Pro Ala Thr Tyr Tyr Asp Ser Leu Ala Val Met Gly Ser Val 
        275                 280                 285             


Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys Val Tyr 
    290                 295                 300                 


Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu Ile Ile 
305                 310                 315                 320 


Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val Met His 
                325                 330                 335     


Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile Asn Gln 
            340                 345                 350         


Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln Glu Asp 
        355                 360                 365             


Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe Asp Ala 
    370                 375                 380                 


Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly Leu Gly 
385                 390                 395                 400 


Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe Ile Gly 
                405                 410                 415     


Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu Ser His 
            420                 425                 430         


Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn Asn Val 
        435                 440                 445             


Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val Thr Trp 
    450                 455                 460                 


Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val Gln Gly 
465                 470                 475                 480 


His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser Glu Asp 
                485                 490                 495     


Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln Phe Gln 
            500                 505                 510         


Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile Trp Cys 
        515                 520                 525             


Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp Asn Gln 
    530                 535                 540                 


Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala Leu His 
545                 550                 555                 560 


Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser Ile Leu 
                565                 570                 575     


Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly Phe Gly 
            580                 585                 590         


Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys Ile Gly 
        595                 600                 605             


Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile His Glu 
    610                 615                 620                 


Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe Gly Gly 
625                 630                 635                 640 


Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro Arg His 
                645                 650                 655     


Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln Lys His 
            660                 665                 670         


Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val Ile Leu 
        675                 680                 685             


Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln Pro Gly 
    690                 695                 700                 


Ala Lys Ala Gln 
705             


<210>  17
<211>  945
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(945)
<223>  LPAAT1

<300>
<302>  A MORTIERELLA ALPINA LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE FOR 
       IMPROVEMENT OF LONG CHAIN OMEGA-3 AND OMEGA-6 POLYUNSATURATED 
       FATTY ACID BIOSYNTHESIS IN OLEAGINOUS ORGANISMS
<310>  US 7,879,591
<311>  2009-06-16
<312>  2011-02-01
<313>  (1)..(945)

<400>  17
atg tcc ata ggt tct tcc aat cct gtc ctg ctg gca gcg atc ccc ttc         48
Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro Phe           
1               5                   10                  15                

gtc tac ctc ttc gtc ctc cct cgt gtc ctc gcc ttc ctc cct caa aag         96
Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln Lys           
            20                  25                  30                    

gcc cag ttc ctc gca aaa tgc atc gtg gtc ttg atc gcc acc ctt atc        144
Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu Ile           
        35                  40                  45                        

atg tcc gtc gca ggc tgc ttc att tcc atc gtc tgt gcg ctc ctc gat        192
Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu Asp           
    50                  55                  60                            

aaa cgc tat gtg atc aac tac gtc gtc tca aga ctc ttc tca ttc ctc        240
Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe Leu           
65                  70                  75                  80            

gct gca aga ccc tgc ggt gtc acc tac aag atc gtc ggc gag gaa cat        288
Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu His           
                85                  90                  95                

ctg gac aag tac ccc gcc att gtc gtc tgc aac cac cag agc tcc atg        336
Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser Met           
            100                 105                 110                   

gac atg atg gtc ctg gga cgc gtc ttc cca aag cac tgt gtc gtc atg        384
Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val Met           
        115                 120                 125                       

gca aag aag gaa ctt ctt tac ttt ccg ttc ctg ggc atg ttt atg aag        432
Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met Lys           
    130                 135                 140                           

ctg agt aac gcc atc ttc att gac cgc aag aac cac aag aag gcg atc        480
Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala Ile           
145                 150                 155                 160           

gag tcc acc acc caa gct gtc gcc gac atg aag aag cac aac tct gga        528
Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser Gly           
                165                 170                 175               

atc tgg att ttc ccc gaa gga aca cgt tcc cgc ttg gac aag gcc gat        576
Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala Asp           
            180                 185                 190                   

ctc ttg ccc ttc aag aag gga gcc ttc cac ctc gcc att caa gcc caa        624
Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala Gln           
        195                 200                 205                       

ctc ccg atc ctc ccc atc atc tcg caa gga tac tca cac atc tac gat        672
Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr Asp           
    210                 215                 220                           

tcg tca aaa cgc tac ttc ccc ggt gga gag ctc gag atc aga gtc ctg        720
Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val Leu           
225                 230                 235                 240           

gaa cct atc ccc acc acg gga ttg acc aca gac gat gtg aac gac ctg        768
Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp Leu           
                245                 250                 255               

atg gac aag act cgc aac ctg atg ctg aag cac ctc aag gag atg gat        816
Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met Asp           
            260                 265                 270                   

tct caa tac tcc tcc tcc acc gct gaa aac gga tcg acc cat att gac        864
Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile Asp           
        275                 280                 285                       

gcc gat atc gca aag tca act gcc aca tcg atc gga aac acg gac gat        912
Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp Asp           
    290                 295                 300                           

gct atc aca aag agg agg aca cca aaa gag tag                            945
Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu                                   
305                 310                                                   


<210>  18
<211>  314
<212>  PRT
<213>  Mortierella alpina

<400>  18

Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro Phe 
1               5                   10                  15      


Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln Lys 
            20                  25                  30          


Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu Ile 
        35                  40                  45              


Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu Asp 
    50                  55                  60                  


Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe Leu 
65                  70                  75                  80  


Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu His 
                85                  90                  95      


Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser Met 
            100                 105                 110         


Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val Met 
        115                 120                 125             


Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met Lys 
    130                 135                 140                 


Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala Ile 
145                 150                 155                 160 


Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser Gly 
                165                 170                 175     


Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala Asp 
            180                 185                 190         


Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala Gln 
        195                 200                 205             


Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr Asp 
    210                 215                 220                 


Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val Leu 
225                 230                 235                 240 


Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp Leu 
                245                 250                 255     


Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met Asp 
            260                 265                 270         


Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile Asp 
        275                 280                 285             


Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp Asp 
    290                 295                 300                 


Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu 
305                 310                 


<210>  19
<211>  955
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (3)..(947)
<223>  synthetic LPAAT1 (codon-optimized for Yarrowia lipolytica)

<300>
<302>  A MORTIERELLA ALPINA LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE FOR 
       IMPROVEMENT OF LONG CHAIN OMEGA-3 AND OMEGA-6 POLYUNSATURATED 
       FATTY ACID BIOSYNTHESIS IN OLEAGINOUS ORGANISMS
<310>  US 7,879,591
<311>  2009-06-16
<312>  2011-02-01
<313>  (1)..(955)

<400>  19
ac atg tct att ggt tcg tcc aac ccc gtg ctc ttg gct gcg att ccc          47
   Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro            
   1               5                   10                  15             

ttc gtc tac ctg ttt gtc ctc cca cga gtc ctg gct ttc ctg cct cag         95
Phe Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln           
                20                  25                  30                

aag gct cag ttc ctg gcc aaa tgt att gtg gtc ctg att gcc acg ctt        143
Lys Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu           
            35                  40                  45                    

atc atg tcc gtt gca ggc tgc ttc atc tcg atc gtg tgc gct ctt ctg        191
Ile Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu           
        50                  55                  60                        

gac aag aga tac gtc atc aat tac gtt gtg tcg cga ttg ttc tcc ttc        239
Asp Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe           
    65                  70                  75                            

ctt gcc gct cga ccg tgt ggt gtg acc tat aag att gtt ggt gag gaa        287
Leu Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu           
80                  85                  90                  95            

cac ctc gat aag tac cct gct atc gtg gtc tgt aac cat caa tcc tct        335
His Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser           
                100                 105                 110               

atg gat atg atg gtt ttg gga cga gtt ttt cca aag cac tgc gtt gtc        383
Met Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val           
            115                 120                 125                   

atg gcg aag aag gaa ctc ctg tac ttt ccc ttt ttg gga atg ttt atg        431
Met Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met           
        130                 135                 140                       

aaa ctg agc aac gct atc ttc atc gac cgg aag aac cac aag aaa gcc        479
Lys Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala           
    145                 150                 155                           

atc gag tct acc acc caa gcc gtg gcg gac atg aag aag cac aac tct        527
Ile Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser           
160                 165                 170                 175           

gga atc tgg att ttc cca gag ggc acc cgg tct aga ctg gac aag gca        575
Gly Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala           
                180                 185                 190               

gac ctg ctg ccc ttc aag aaa ggt gcc ttt cat ctt gca att cag gcc        623
Asp Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala           
            195                 200                 205                   

cag ctc cct att ctc ccc att atc tcg cag ggc tat tcc cat atc tac        671
Gln Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr           
        210                 215                 220                       

gac tct tcg aag cgg tac ttc ccc ggt gga gag ctc gag atc aga gtc        719
Asp Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val           
    225                 230                 235                           

ctg gag ccc att cct aca act ggc ctc act act gat gat gtg aac gac        767
Leu Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp           
240                 245                 250                 255           

ctg atg gac aag aca cga aac ctt atg ctc aag cac ttg aag gag atg        815
Leu Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met           
                260                 265                 270               

gat tcc cag tat tcg tcg agc act gct gaa aat gga tcc acg cac atc        863
Asp Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile           
            275                 280                 285                   

gac gcc gat att gcc aag tct aca gcc acc agc att ggc aac act gac        911
Asp Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp           
        290                 295                 300                       

gac gca att aca aaa cgt cgt acc cct aag gaa taa gcggccgc               955
Asp Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu                               
    305                 310                                               


<210>  20
<211>  314
<212>  PRT
<213>  Mortierella alpina

<400>  20

Met Ser Ile Gly Ser Ser Asn Pro Val Leu Leu Ala Ala Ile Pro Phe 
1               5                   10                  15      


Val Tyr Leu Phe Val Leu Pro Arg Val Leu Ala Phe Leu Pro Gln Lys 
            20                  25                  30          


Ala Gln Phe Leu Ala Lys Cys Ile Val Val Leu Ile Ala Thr Leu Ile 
        35                  40                  45              


Met Ser Val Ala Gly Cys Phe Ile Ser Ile Val Cys Ala Leu Leu Asp 
    50                  55                  60                  


Lys Arg Tyr Val Ile Asn Tyr Val Val Ser Arg Leu Phe Ser Phe Leu 
65                  70                  75                  80  


Ala Ala Arg Pro Cys Gly Val Thr Tyr Lys Ile Val Gly Glu Glu His 
                85                  90                  95      


Leu Asp Lys Tyr Pro Ala Ile Val Val Cys Asn His Gln Ser Ser Met 
            100                 105                 110         


Asp Met Met Val Leu Gly Arg Val Phe Pro Lys His Cys Val Val Met 
        115                 120                 125             


Ala Lys Lys Glu Leu Leu Tyr Phe Pro Phe Leu Gly Met Phe Met Lys 
    130                 135                 140                 


Leu Ser Asn Ala Ile Phe Ile Asp Arg Lys Asn His Lys Lys Ala Ile 
145                 150                 155                 160 


Glu Ser Thr Thr Gln Ala Val Ala Asp Met Lys Lys His Asn Ser Gly 
                165                 170                 175     


Ile Trp Ile Phe Pro Glu Gly Thr Arg Ser Arg Leu Asp Lys Ala Asp 
            180                 185                 190         


Leu Leu Pro Phe Lys Lys Gly Ala Phe His Leu Ala Ile Gln Ala Gln 
        195                 200                 205             


Leu Pro Ile Leu Pro Ile Ile Ser Gln Gly Tyr Ser His Ile Tyr Asp 
    210                 215                 220                 


Ser Ser Lys Arg Tyr Phe Pro Gly Gly Glu Leu Glu Ile Arg Val Leu 
225                 230                 235                 240 


Glu Pro Ile Pro Thr Thr Gly Leu Thr Thr Asp Asp Val Asn Asp Leu 
                245                 250                 255     


Met Asp Lys Thr Arg Asn Leu Met Leu Lys His Leu Lys Glu Met Asp 
            260                 265                 270         


Ser Gln Tyr Ser Ser Ser Thr Ala Glu Asn Gly Ser Thr His Ile Asp 
        275                 280                 285             


Ala Asp Ile Ala Lys Ser Thr Ala Thr Ser Ile Gly Asn Thr Asp Asp 
    290                 295                 300                 


Ala Ile Thr Lys Arg Arg Thr Pro Lys Glu 
305                 310                 


<210>  21
<211>  849
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(849)

<300>
<302>  A MORTIERELLA ALPINA LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE 
       HOMOLOG FOR ALTERATION OF POLYUNSATURATED FATTY ACIDS AND OIL 
       CONTENT IN OLEAGINOUS ORGANISMS
<310>  US7189559
<311>  2005-10-14
<312>  2007-03-13
<313>  (1)..(849)

<400>  21
atg tcc gtt gca tcc aag ctc gtc ttc tac gtc cgc gcc gcc atc gcc         48
Met Ser Val Ala Ser Lys Leu Val Phe Tyr Val Arg Ala Ala Ile Ala           
1               5                   10                  15                

gtg gtc atc ttt gcc gcc tgt gcc acc tac ggc gtg ctg gcg tcc acc         96
Val Val Ile Phe Ala Ala Cys Ala Thr Tyr Gly Val Leu Ala Ser Thr           
            20                  25                  30                    

att ctc acc gcc atc ggc aag cag ggc ctg gcc caa tgg acc gtt gcc        144
Ile Leu Thr Ala Ile Gly Lys Gln Gly Leu Ala Gln Trp Thr Val Ala           
        35                  40                  45                        

aga gcc ttc tac tac tcg gtg cgc atc ttc ctg ggt atc agc atc aag        192
Arg Ala Phe Tyr Tyr Ser Val Arg Ile Phe Leu Gly Ile Ser Ile Lys           
    50                  55                  60                            

ctg cgt agc cgg cag gtg acc gga acc gcc ggt ctg gat gcc tcc aag        240
Leu Arg Ser Arg Gln Val Thr Gly Thr Ala Gly Leu Asp Ala Ser Lys           
65                  70                  75                  80            

atc cag gtc gcc aac acc acc aag ccc att gac gac atc acc aaa cac        288
Ile Gln Val Ala Asn Thr Thr Lys Pro Ile Asp Asp Ile Thr Lys His           
                85                  90                  95                

ctg ccc cga cca tgc att ctg att tcc aac cac cag aac gaa atg gac        336
Leu Pro Arg Pro Cys Ile Leu Ile Ser Asn His Gln Asn Glu Met Asp           
            100                 105                 110                   

att ctg gtg ctc ggt cgc atc ttc ccc cag tac tgc tcc gtc acc gcc        384
Ile Leu Val Leu Gly Arg Ile Phe Pro Gln Tyr Cys Ser Val Thr Ala           
        115                 120                 125                       

aaa aag gcc ctc aag tgg tac cct ctg ctg ggc cag ttc atg gcg ctg        432
Lys Lys Ala Leu Lys Trp Tyr Pro Leu Leu Gly Gln Phe Met Ala Leu           
    130                 135                 140                           

tcc ggc acc atc ttc ctg gac cga aag gac cga acc aag tcc gtg cag        480
Ser Gly Thr Ile Phe Leu Asp Arg Lys Asp Arg Thr Lys Ser Val Gln           
145                 150                 155                 160           

acc ctc ggc ggc gcc gtc aag acc atc cag agc ggc aac gga ggc aag        528
Thr Leu Gly Gly Ala Val Lys Thr Ile Gln Ser Gly Asn Gly Gly Lys           
                165                 170                 175               

ggc cag agc gtc ttc atg ttc ccc gag gga acc cga tcc tac tcc aag        576
Gly Gln Ser Val Phe Met Phe Pro Glu Gly Thr Arg Ser Tyr Ser Lys           
            180                 185                 190                   

gac gtc ggc atc atg ccc ttc aag aag ggc tgt ttc cac ctg gcg gtc        624
Asp Val Gly Ile Met Pro Phe Lys Lys Gly Cys Phe His Leu Ala Val           
        195                 200                 205                       

cag tcg ggc gct ccc att gtc ccc gtg gtg gtc cag aac acc tcc cga        672
Gln Ser Gly Ala Pro Ile Val Pro Val Val Val Gln Asn Thr Ser Arg           
    210                 215                 220                           

atg ttt tct ttc ggc cga ggc aag ctg gac gcc gga gag atc ctt gtc        720
Met Phe Ser Phe Gly Arg Gly Lys Leu Asp Ala Gly Glu Ile Leu Val           
225                 230                 235                 240           

gac gtc ctg agc ccc att gag acc aag ggt ctg gac gcc agc aac gtc        768
Asp Val Leu Ser Pro Ile Glu Thr Lys Gly Leu Asp Ala Ser Asn Val           
                245                 250                 255               

gac gct ctc atg gcc acc act tat aag gcc atg tgc gag act gcc gac        816
Asp Ala Leu Met Ala Thr Thr Tyr Lys Ala Met Cys Glu Thr Ala Asp           
            260                 265                 270                   

cag att ggc tac gct ggc cag aag act cag tag                            849
Gln Ile Gly Tyr Ala Gly Gln Lys Thr Gln                                   
        275                 280                                           


<210>  22
<211>  282
<212>  PRT
<213>  Yarrowia lipolytica

<400>  22

Met Ser Val Ala Ser Lys Leu Val Phe Tyr Val Arg Ala Ala Ile Ala 
1               5                   10                  15      


Val Val Ile Phe Ala Ala Cys Ala Thr Tyr Gly Val Leu Ala Ser Thr 
            20                  25                  30          


Ile Leu Thr Ala Ile Gly Lys Gln Gly Leu Ala Gln Trp Thr Val Ala 
        35                  40                  45              


Arg Ala Phe Tyr Tyr Ser Val Arg Ile Phe Leu Gly Ile Ser Ile Lys 
    50                  55                  60                  


Leu Arg Ser Arg Gln Val Thr Gly Thr Ala Gly Leu Asp Ala Ser Lys 
65                  70                  75                  80  


Ile Gln Val Ala Asn Thr Thr Lys Pro Ile Asp Asp Ile Thr Lys His 
                85                  90                  95      


Leu Pro Arg Pro Cys Ile Leu Ile Ser Asn His Gln Asn Glu Met Asp 
            100                 105                 110         


Ile Leu Val Leu Gly Arg Ile Phe Pro Gln Tyr Cys Ser Val Thr Ala 
        115                 120                 125             


Lys Lys Ala Leu Lys Trp Tyr Pro Leu Leu Gly Gln Phe Met Ala Leu 
    130                 135                 140                 


Ser Gly Thr Ile Phe Leu Asp Arg Lys Asp Arg Thr Lys Ser Val Gln 
145                 150                 155                 160 


Thr Leu Gly Gly Ala Val Lys Thr Ile Gln Ser Gly Asn Gly Gly Lys 
                165                 170                 175     


Gly Gln Ser Val Phe Met Phe Pro Glu Gly Thr Arg Ser Tyr Ser Lys 
            180                 185                 190         


Asp Val Gly Ile Met Pro Phe Lys Lys Gly Cys Phe His Leu Ala Val 
        195                 200                 205             


Gln Ser Gly Ala Pro Ile Val Pro Val Val Val Gln Asn Thr Ser Arg 
    210                 215                 220                 


Met Phe Ser Phe Gly Arg Gly Lys Leu Asp Ala Gly Glu Ile Leu Val 
225                 230                 235                 240 


Asp Val Leu Ser Pro Ile Glu Thr Lys Gly Leu Asp Ala Ser Asn Val 
                245                 250                 255     


Asp Ala Leu Met Ala Thr Thr Tyr Lys Ala Met Cys Glu Thr Ala Asp 
            260                 265                 270         


Gln Ile Gly Tyr Ala Gly Gln Lys Thr Gln 
        275                 280         


<210>  23
<211>  303
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<222>  (1)..(303)
<223>  Slc1p; GenBank Accession No.  NP_010231

<400>  23

Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu Val 
1               5                   10                  15      


Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser Ile 
            20                  25                  30          


Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr Ala 
        35                  40                  45              


Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val Lys 
    50                  55                  60                  


Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile Ala 
65                  70                  75                  80  


Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe Pro 
                85                  90                  95      


Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro Phe 
            100                 105                 110         


Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg Ser 
        115                 120                 125             


Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn Val 
    130                 135                 140                 


Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg Ser 
145                 150                 155                 160 


Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe His 
                165                 170                 175     


Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser Asn 
            180                 185                 190         


Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly Cys 
        195                 200                 205             


Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr Lys 
    210                 215                 220                 


Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val Asp 
225                 230                 235                 240 


Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr Leu 
                245                 250                 255     


Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys Val 
            260                 265                 270         


Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser Asn 
        275                 280                 285             


Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His 
    290                 295                 300             


<210>  24
<211>  926
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (4)..(915)

<400>  24
aac atg tct gtt att gga cga ttt ctt tac tac ctg aga tcg gtg ctc         48
    Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu           
    1               5                   10                  15            

gtc gtt ttg gcc ctc gct gga tgt ggc ttc tat ggc gtg att gcc tct         96
Val Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser           
                20                  25                  30                

atc ctg tgt act ctc atc ggc aag cag cat ctc gcg caa tgg att acc        144
Ile Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr           
            35                  40                  45                    

gcc cga tgc ttt tac cac gtg atg aaa ctg atg ctg gga ttg gac gtc        192
Ala Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val           
        50                  55                  60                        

aaa gtc gtg ggt gaa gag aac ctg gct aag aag ccc tat atc atg atc        240
Lys Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile           
    65                  70                  75                            

gct aac cac cag tcc acc ctc gat atc ttt atg ctc ggc aga atc ttc        288
Ala Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe           
80                  85                  90                  95            

cct ccc gga tgc acc gtt acc gca aag aag agc ctt aag tac gtc ccc        336
Pro Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro           
                100                 105                 110               

ttc ctg ggc tgg ttt atg gcg ctt tcc ggt aca tac ttc ctc gac cgt        384
Phe Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg           
            115                 120                 125                   

tcc aag cgg caa gag gct att gat acc ctg aac aaa gga ctg gag aac        432
Ser Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn           
        130                 135                 140                       

gtc aaa aag aat aag cga gcc ctc tgg gtt ttt ccc gaa ggt acc cgg        480
Val Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg           
    145                 150                 155                           

tct tac acg tcg gag ctt acc atg ctg ccg ttc aag aag ggc gcc ttt        528
Ser Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe           
160                 165                 170                 175           

cat ttg gcc cag cag gga aag atc cct atc gtc cca gtg gtt gtg tct        576
His Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser           
                180                 185                 190               

aac act agc acg ctc gtc agc cct aag tac ggt gtg ttc aac cga ggc        624
Asn Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly           
            195                 200                 205                   

tgt atg att gtc cga att ctg aag ccc atc tcg act gag aac ctg aca        672
Cys Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr           
        210                 215                 220                       

aaa gat aag att ggc gag ttc gct gag aaa gtg cga gat cag atg gtt        720
Lys Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val           
    225                 230                 235                           

gac aca ctg aag gaa atc ggt tat tcc ccc gca atc aac gac acc acc        768
Asp Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr           
240                 245                 250                 255           

ctt cct ccg cag gca att gag tac gcc gca ttg cag cat gac aag aag        816
Leu Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys           
                260                 265                 270               

gtg aac aaa aag att aag aac gaa cct gtt cca tcg gtg tcc att tct        864
Val Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser           
            275                 280                 285                   

aat gat gtg aat act cac aac gaa gga tcg tct gtc aag aag atg cac        912
Asn Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His           
        290                 295                 300                       

tag gcggccgcat g                                                       926


<210>  25
<211>  303
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  25

Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu Val 
1               5                   10                  15      


Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser Ile 
            20                  25                  30          


Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr Ala 
        35                  40                  45              


Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val Lys 
    50                  55                  60                  


Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile Ala 
65                  70                  75                  80  


Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe Pro 
                85                  90                  95      


Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro Phe 
            100                 105                 110         


Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg Ser 
        115                 120                 125             


Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn Val 
    130                 135                 140                 


Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg Ser 
145                 150                 155                 160 


Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe His 
                165                 170                 175     


Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser Asn 
            180                 185                 190         


Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly Cys 
        195                 200                 205             


Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr Lys 
    210                 215                 220                 


Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val Asp 
225                 230                 235                 240 


Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr Leu 
                245                 250                 255     


Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys Val 
            260                 265                 270         


Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser Asn 
        275                 280                 285             


Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His 
    290                 295                 300             


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif


<220>
<221>  misc_feature
<222>  (3)..(6)
<223>  Xaa can be any naturally occurring amino acid

<400>  26

Asn His Xaa Xaa Xaa Xaa Asp 
1               5           


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1-acyl-sn-glycerol-3-phosphate acyltransferase motif

<400>  27

Glu Gly Thr Arg 
1               


<210>  28
<211>  1947
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1947)
<223>  phospholipid:diacylglycerol acyltransferase

<300>
<302>  ACYLTRANSFERASES FOR ALTERATION OF POLYUNSATURATED FATTY ACIDS 
       AND OIL CONTENT IN OLEAGINOUS YEASTS
<310>  US 7,901,928
<311>  2009-03-11
<312>  2011-03-08
<313>  (1)..(1947)

<400>  28
atg aca caa cct gtg aat cgg aag gcg act gtc gag cgg gtc gag cca         48
Met Thr Gln Pro Val Asn Arg Lys Ala Thr Val Glu Arg Val Glu Pro           
1               5                   10                  15                

gca gtg gag gtg gct gac tcc gag tcc gag gcc aag acc gac gtc cac         96
Ala Val Glu Val Ala Asp Ser Glu Ser Glu Ala Lys Thr Asp Val His           
            20                  25                  30                    

gtt cac cac cat cat cac cac cac aag cga aaa tcc gtc aag ggc aag        144
Val His His His His His His His Lys Arg Lys Ser Val Lys Gly Lys           
        35                  40                  45                        

att ctc aac ttc ttc acc cga agt cga cgt atc acc ttc gtc ctc ggc        192
Ile Leu Asn Phe Phe Thr Arg Ser Arg Arg Ile Thr Phe Val Leu Gly           
    50                  55                  60                            

gcc gtg gtc ggt gtg ata gcc gcg gga tac tac gct gcg cca ccg gag        240
Ala Val Val Gly Val Ile Ala Ala Gly Tyr Tyr Ala Ala Pro Pro Glu           
65                  70                  75                  80            

ctc agc att gat atc gat gct ctt ctc ggc gac ttg ccc tcg ttc gac        288
Leu Ser Ile Asp Ile Asp Ala Leu Leu Gly Asp Leu Pro Ser Phe Asp           
                85                  90                  95                

ttt gac gct cta tct ctc gac aac ttg tcc atg gac agt gtg tcg gac        336
Phe Asp Ala Leu Ser Leu Asp Asn Leu Ser Met Asp Ser Val Ser Asp           
            100                 105                 110                   

ttt gta caa gac atg aaa tcg cgg ttt ccg acc aag att ctg cag gag        384
Phe Val Gln Asp Met Lys Ser Arg Phe Pro Thr Lys Ile Leu Gln Glu           
        115                 120                 125                       

gcg gcc aag atc gag aag cac cag aaa agc gaa cag aag gct gcc cct        432
Ala Ala Lys Ile Glu Lys His Gln Lys Ser Glu Gln Lys Ala Ala Pro           
    130                 135                 140                           

ttt gct gtg ggc aag gct atg aag agc gag gga ctc aac gcc aag tac        480
Phe Ala Val Gly Lys Ala Met Lys Ser Glu Gly Leu Asn Ala Lys Tyr           
145                 150                 155                 160           

ccg gtg gtg ctg gtg ccc ggc gtc atc tcc acg gga ctg gag agc tgg        528
Pro Val Val Leu Val Pro Gly Val Ile Ser Thr Gly Leu Glu Ser Trp           
                165                 170                 175               

tcc ctg gag gga acc gag gag tgt ccc acc gag tcg cac ttc aga aag        576
Ser Leu Glu Gly Thr Glu Glu Cys Pro Thr Glu Ser His Phe Arg Lys           
            180                 185                 190                   

cga atg tgg ggc tcc tgg tac atg atc cga gtc atg ctg ctg gac aag        624
Arg Met Trp Gly Ser Trp Tyr Met Ile Arg Val Met Leu Leu Asp Lys           
        195                 200                 205                       

tac tgc tgg ctg cag aac ctg atg ctg gac aca gag acc ggt cta gac        672
Tyr Cys Trp Leu Gln Asn Leu Met Leu Asp Thr Glu Thr Gly Leu Asp           
    210                 215                 220                           

cct ccc cat ttc aag ctg cga gcc gcc cag gga ttt gcc tcc gcc gac        720
Pro Pro His Phe Lys Leu Arg Ala Ala Gln Gly Phe Ala Ser Ala Asp           
225                 230                 235                 240           

ttc ttt atg gca ggc tac tgg ctg tgg aac aag ctg ctc gag aac ctg        768
Phe Phe Met Ala Gly Tyr Trp Leu Trp Asn Lys Leu Leu Glu Asn Leu           
                245                 250                 255               

gct gtt att gga tac gat acg gat aca atg tct gct gcg gcg tac gac        816
Ala Val Ile Gly Tyr Asp Thr Asp Thr Met Ser Ala Ala Ala Tyr Asp           
            260                 265                 270                   

tgg aga ctg tcc tac cct gat ttg gag cac cga gac gga tac ttc tcc        864
Trp Arg Leu Ser Tyr Pro Asp Leu Glu His Arg Asp Gly Tyr Phe Ser           
        275                 280                 285                       

aag ctc aaa gct tca atc gaa gag act aag cgt atg aca ggt gag aag        912
Lys Leu Lys Ala Ser Ile Glu Glu Thr Lys Arg Met Thr Gly Glu Lys           
    290                 295                 300                           

aca gtt ctg acg ggc cat tcc atg ggc tcc cag gtc atc ttc tac ttc        960
Thr Val Leu Thr Gly His Ser Met Gly Ser Gln Val Ile Phe Tyr Phe           
305                 310                 315                 320           

atg aag tgg gct gag gcc gag gga tat gga gga gga ggt ccc aac tgg       1008
Met Lys Trp Ala Glu Ala Glu Gly Tyr Gly Gly Gly Gly Pro Asn Trp           
                325                 330                 335               

gtc aat gac cat att gaa tcc ttt gtc gac att tcc ggc tcc atg ctg       1056
Val Asn Asp His Ile Glu Ser Phe Val Asp Ile Ser Gly Ser Met Leu           
            340                 345                 350                   

ggt act ccc aag acc ctg gtt gct ctt ctg tct gga gaa atg aag gat       1104
Gly Thr Pro Lys Thr Leu Val Ala Leu Leu Ser Gly Glu Met Lys Asp           
        355                 360                 365                       

acc gtg cag ctg aac gcg atg gct gtg tat gga ctg gag cag ttc ttc       1152
Thr Val Gln Leu Asn Ala Met Ala Val Tyr Gly Leu Glu Gln Phe Phe           
    370                 375                 380                           

tct cga cga gag cga gcc gat ctg ctg cga aca tgg gga gga att gct       1200
Ser Arg Arg Glu Arg Ala Asp Leu Leu Arg Thr Trp Gly Gly Ile Ala           
385                 390                 395                 400           

tcc atg att ccc aag ggt ggt aag gct atc tgg ggt gat cat tct gga       1248
Ser Met Ile Pro Lys Gly Gly Lys Ala Ile Trp Gly Asp His Ser Gly           
                405                 410                 415               

gcc cct gat gac gag ccc ggc cag aat gtc acc ttt ggc aac ttc atc       1296
Ala Pro Asp Asp Glu Pro Gly Gln Asn Val Thr Phe Gly Asn Phe Ile           
            420                 425                 430                   

aag ttc aag gag tcc ttg acc gag tac tct gct aag aac ctc acc atg       1344
Lys Phe Lys Glu Ser Leu Thr Glu Tyr Ser Ala Lys Asn Leu Thr Met           
        435                 440                 445                       

gat gaa acc gtt gac ttc ctg tat tct cag tct ccc gag tgg ttt gtg       1392
Asp Glu Thr Val Asp Phe Leu Tyr Ser Gln Ser Pro Glu Trp Phe Val           
    450                 455                 460                           

aac cga acc gag ggt gct tac tcc ttt gga att gcc aag act cga aag       1440
Asn Arg Thr Glu Gly Ala Tyr Ser Phe Gly Ile Ala Lys Thr Arg Lys           
465                 470                 475                 480           

cag gtt gag cag aat gag aag cga cct tct acc tgg agc aac cct ctg       1488
Gln Val Glu Gln Asn Glu Lys Arg Pro Ser Thr Trp Ser Asn Pro Leu           
                485                 490                 495               

gaa gct gct ctc ccc aat gcc ccc gat ctc aag atc tac tgc ttc tat       1536
Glu Ala Ala Leu Pro Asn Ala Pro Asp Leu Lys Ile Tyr Cys Phe Tyr           
            500                 505                 510                   

gga gtc ggt aag gat acc gag cga gcc tac tac tac cag gat gag ccc       1584
Gly Val Gly Lys Asp Thr Glu Arg Ala Tyr Tyr Tyr Gln Asp Glu Pro           
        515                 520                 525                       

aat ccc gag cag acc aac ttg aac gtc agt atc gct gga aac gac cct       1632
Asn Pro Glu Gln Thr Asn Leu Asn Val Ser Ile Ala Gly Asn Asp Pro           
    530                 535                 540                           

gat ggt gtg ctt atg ggt cag ggc gat gga acc gtc tcc ctt gtg acc       1680
Asp Gly Val Leu Met Gly Gln Gly Asp Gly Thr Val Ser Leu Val Thr           
545                 550                 555                 560           

cat acc atg tgt cac cga tgg aag gac gag aat tcc aag ttc aac cct       1728
His Thr Met Cys His Arg Trp Lys Asp Glu Asn Ser Lys Phe Asn Pro           
                565                 570                 575               

ggt aac gcc cag gtc aag gtt gtg gag atg ttg cac cag cct gat cga       1776
Gly Asn Ala Gln Val Lys Val Val Glu Met Leu His Gln Pro Asp Arg           
            580                 585                 590                   

ctt gat att cga ggc ggt gct cag act gcc gag cat gtg gac att ctg       1824
Leu Asp Ile Arg Gly Gly Ala Gln Thr Ala Glu His Val Asp Ile Leu           
        595                 600                 605                       

ggg cgt tct gag ttg aac gag atg gtt ctg aag gtg gct agt gga aag       1872
Gly Arg Ser Glu Leu Asn Glu Met Val Leu Lys Val Ala Ser Gly Lys           
    610                 615                 620                           

gga aat gag att gaa gag aga gtc atc tcc aac att gat gag tgg gtg       1920
Gly Asn Glu Ile Glu Glu Arg Val Ile Ser Asn Ile Asp Glu Trp Val           
625                 630                 635                 640           

tgg aag att gat ctc ggc agc aat tag                                   1947
Trp Lys Ile Asp Leu Gly Ser Asn                                           
                645                                                       


<210>  29
<211>  648
<212>  PRT
<213>  Yarrowia lipolytica

<400>  29

Met Thr Gln Pro Val Asn Arg Lys Ala Thr Val Glu Arg Val Glu Pro 
1               5                   10                  15      


Ala Val Glu Val Ala Asp Ser Glu Ser Glu Ala Lys Thr Asp Val His 
            20                  25                  30          


Val His His His His His His His Lys Arg Lys Ser Val Lys Gly Lys 
        35                  40                  45              


Ile Leu Asn Phe Phe Thr Arg Ser Arg Arg Ile Thr Phe Val Leu Gly 
    50                  55                  60                  


Ala Val Val Gly Val Ile Ala Ala Gly Tyr Tyr Ala Ala Pro Pro Glu 
65                  70                  75                  80  


Leu Ser Ile Asp Ile Asp Ala Leu Leu Gly Asp Leu Pro Ser Phe Asp 
                85                  90                  95      


Phe Asp Ala Leu Ser Leu Asp Asn Leu Ser Met Asp Ser Val Ser Asp 
            100                 105                 110         


Phe Val Gln Asp Met Lys Ser Arg Phe Pro Thr Lys Ile Leu Gln Glu 
        115                 120                 125             


Ala Ala Lys Ile Glu Lys His Gln Lys Ser Glu Gln Lys Ala Ala Pro 
    130                 135                 140                 


Phe Ala Val Gly Lys Ala Met Lys Ser Glu Gly Leu Asn Ala Lys Tyr 
145                 150                 155                 160 


Pro Val Val Leu Val Pro Gly Val Ile Ser Thr Gly Leu Glu Ser Trp 
                165                 170                 175     


Ser Leu Glu Gly Thr Glu Glu Cys Pro Thr Glu Ser His Phe Arg Lys 
            180                 185                 190         


Arg Met Trp Gly Ser Trp Tyr Met Ile Arg Val Met Leu Leu Asp Lys 
        195                 200                 205             


Tyr Cys Trp Leu Gln Asn Leu Met Leu Asp Thr Glu Thr Gly Leu Asp 
    210                 215                 220                 


Pro Pro His Phe Lys Leu Arg Ala Ala Gln Gly Phe Ala Ser Ala Asp 
225                 230                 235                 240 


Phe Phe Met Ala Gly Tyr Trp Leu Trp Asn Lys Leu Leu Glu Asn Leu 
                245                 250                 255     


Ala Val Ile Gly Tyr Asp Thr Asp Thr Met Ser Ala Ala Ala Tyr Asp 
            260                 265                 270         


Trp Arg Leu Ser Tyr Pro Asp Leu Glu His Arg Asp Gly Tyr Phe Ser 
        275                 280                 285             


Lys Leu Lys Ala Ser Ile Glu Glu Thr Lys Arg Met Thr Gly Glu Lys 
    290                 295                 300                 


Thr Val Leu Thr Gly His Ser Met Gly Ser Gln Val Ile Phe Tyr Phe 
305                 310                 315                 320 


Met Lys Trp Ala Glu Ala Glu Gly Tyr Gly Gly Gly Gly Pro Asn Trp 
                325                 330                 335     


Val Asn Asp His Ile Glu Ser Phe Val Asp Ile Ser Gly Ser Met Leu 
            340                 345                 350         


Gly Thr Pro Lys Thr Leu Val Ala Leu Leu Ser Gly Glu Met Lys Asp 
        355                 360                 365             


Thr Val Gln Leu Asn Ala Met Ala Val Tyr Gly Leu Glu Gln Phe Phe 
    370                 375                 380                 


Ser Arg Arg Glu Arg Ala Asp Leu Leu Arg Thr Trp Gly Gly Ile Ala 
385                 390                 395                 400 


Ser Met Ile Pro Lys Gly Gly Lys Ala Ile Trp Gly Asp His Ser Gly 
                405                 410                 415     


Ala Pro Asp Asp Glu Pro Gly Gln Asn Val Thr Phe Gly Asn Phe Ile 
            420                 425                 430         


Lys Phe Lys Glu Ser Leu Thr Glu Tyr Ser Ala Lys Asn Leu Thr Met 
        435                 440                 445             


Asp Glu Thr Val Asp Phe Leu Tyr Ser Gln Ser Pro Glu Trp Phe Val 
    450                 455                 460                 


Asn Arg Thr Glu Gly Ala Tyr Ser Phe Gly Ile Ala Lys Thr Arg Lys 
465                 470                 475                 480 


Gln Val Glu Gln Asn Glu Lys Arg Pro Ser Thr Trp Ser Asn Pro Leu 
                485                 490                 495     


Glu Ala Ala Leu Pro Asn Ala Pro Asp Leu Lys Ile Tyr Cys Phe Tyr 
            500                 505                 510         


Gly Val Gly Lys Asp Thr Glu Arg Ala Tyr Tyr Tyr Gln Asp Glu Pro 
        515                 520                 525             


Asn Pro Glu Gln Thr Asn Leu Asn Val Ser Ile Ala Gly Asn Asp Pro 
    530                 535                 540                 


Asp Gly Val Leu Met Gly Gln Gly Asp Gly Thr Val Ser Leu Val Thr 
545                 550                 555                 560 


His Thr Met Cys His Arg Trp Lys Asp Glu Asn Ser Lys Phe Asn Pro 
                565                 570                 575     


Gly Asn Ala Gln Val Lys Val Val Glu Met Leu His Gln Pro Asp Arg 
            580                 585                 590         


Leu Asp Ile Arg Gly Gly Ala Gln Thr Ala Glu His Val Asp Ile Leu 
        595                 600                 605             


Gly Arg Ser Glu Leu Asn Glu Met Val Leu Lys Val Ala Ser Gly Lys 
    610                 615                 620                 


Gly Asn Glu Ile Glu Glu Arg Val Ile Ser Asn Ile Asp Glu Trp Val 
625                 630                 635                 640 


Trp Lys Ile Asp Leu Gly Ser Asn 
                645             


<210>  30
<211>  661
<212>  PRT
<213>  Saccharomyces cerevisiae


<220>
<221>  MISC_FEATURE
<222>  (1)..(661)
<223>  GenBank Accession No. P40345

<400>  30

Met Gly Thr Leu Phe Arg Arg Asn Val Gln Asn Gln Lys Ser Asp Ser 
1               5                   10                  15      


Asp Glu Asn Asn Lys Gly Gly Ser Val His Asn Lys Arg Glu Ser Arg 
            20                  25                  30          


Asn His Ile His His Gln Gln Gly Leu Gly His Lys Arg Arg Arg Gly 
        35                  40                  45              


Ile Ser Gly Ser Ala Lys Arg Asn Glu Arg Gly Lys Asp Phe Asp Arg 
    50                  55                  60                  


Lys Arg Asp Gly Asn Gly Arg Lys Arg Trp Arg Asp Ser Arg Arg Leu 
65                  70                  75                  80  


Ile Phe Ile Leu Gly Ala Phe Leu Gly Val Leu Leu Pro Phe Ser Phe 
                85                  90                  95      


Gly Ala Tyr His Val His Asn Ser Asp Ser Asp Leu Phe Asp Asn Phe 
            100                 105                 110         


Val Asn Phe Asp Ser Leu Lys Val Tyr Leu Asp Asp Trp Lys Asp Val 
        115                 120                 125             


Leu Pro Gln Gly Ile Ser Ser Phe Ile Asp Asp Ile Gln Ala Gly Asn 
    130                 135                 140                 


Tyr Ser Thr Ser Ser Leu Asp Asp Leu Ser Glu Asn Phe Ala Val Gly 
145                 150                 155                 160 


Lys Gln Leu Leu Arg Asp Tyr Asn Ile Glu Ala Lys His Pro Val Val 
                165                 170                 175     


Met Val Pro Gly Val Ile Ser Thr Gly Ile Glu Ser Trp Gly Val Ile 
            180                 185                 190         


Gly Asp Asp Glu Cys Asp Ser Ser Ala His Phe Arg Lys Arg Leu Trp 
        195                 200                 205             


Gly Ser Phe Tyr Met Leu Arg Thr Met Val Met Asp Lys Val Cys Trp 
    210                 215                 220                 


Leu Lys His Val Met Leu Asp Pro Glu Thr Gly Leu Asp Pro Pro Asn 
225                 230                 235                 240 


Phe Thr Leu Arg Ala Ala Gln Gly Phe Glu Ser Thr Asp Tyr Phe Ile 
                245                 250                 255     


Ala Gly Tyr Trp Ile Trp Asn Lys Val Phe Gln Asn Leu Gly Val Ile 
            260                 265                 270         


Gly Tyr Glu Pro Asn Lys Met Thr Ser Ala Ala Tyr Asp Trp Arg Leu 
        275                 280                 285             


Ala Tyr Leu Asp Leu Glu Arg Arg Asp Arg Tyr Phe Thr Lys Leu Lys 
    290                 295                 300                 


Glu Gln Ile Glu Leu Phe His Gln Leu Ser Gly Glu Lys Val Cys Leu 
305                 310                 315                 320 


Ile Gly His Ser Met Gly Ser Gln Ile Ile Phe Tyr Phe Met Lys Trp 
                325                 330                 335     


Val Glu Ala Glu Gly Pro Leu Tyr Gly Asn Gly Gly Arg Gly Trp Val 
            340                 345                 350         


Asn Glu His Ile Asp Ser Phe Ile Asn Ala Ala Gly Thr Leu Leu Gly 
        355                 360                 365             


Ala Pro Lys Ala Val Pro Ala Leu Ile Ser Gly Glu Met Lys Asp Thr 
    370                 375                 380                 


Ile Gln Leu Asn Thr Leu Ala Met Tyr Gly Leu Glu Lys Phe Phe Ser 
385                 390                 395                 400 


Arg Ile Glu Arg Val Lys Met Leu Gln Thr Trp Gly Gly Ile Pro Ser 
                405                 410                 415     


Met Leu Pro Lys Gly Glu Glu Val Ile Trp Gly Asp Met Lys Ser Ser 
            420                 425                 430         


Ser Glu Asp Ala Leu Asn Asn Asn Thr Asp Thr Tyr Gly Asn Phe Ile 
        435                 440                 445             


Arg Phe Glu Arg Asn Thr Ser Asp Ala Phe Asn Lys Asn Leu Thr Met 
    450                 455                 460                 


Lys Asp Ala Ile Asn Met Thr Leu Ser Ile Ser Pro Glu Trp Leu Gln 
465                 470                 475                 480 


Arg Arg Val His Glu Gln Tyr Ser Phe Gly Tyr Ser Lys Asn Glu Glu 
                485                 490                 495     


Glu Leu Arg Lys Asn Glu Leu His His Lys His Trp Ser Asn Pro Met 
            500                 505                 510         


Glu Val Pro Leu Pro Glu Ala Pro His Met Lys Ile Tyr Cys Ile Tyr 
        515                 520                 525             


Gly Val Asn Asn Pro Thr Glu Arg Ala Tyr Val Tyr Lys Glu Glu Asp 
    530                 535                 540                 


Asp Ser Ser Ala Leu Asn Leu Thr Ile Asp Tyr Glu Ser Lys Gln Pro 
545                 550                 555                 560 


Val Phe Leu Thr Glu Gly Asp Gly Thr Val Pro Leu Val Ala His Ser 
                565                 570                 575     


Met Cys His Lys Trp Ala Gln Gly Ala Ser Pro Tyr Asn Pro Ala Gly 
            580                 585                 590         


Ile Asn Val Thr Ile Val Glu Met Lys His Gln Pro Asp Arg Phe Asp 
        595                 600                 605             


Ile Arg Gly Gly Ala Lys Ser Ala Glu His Val Asp Ile Leu Gly Ser 
    610                 615                 620                 


Ala Glu Leu Asn Asp Tyr Ile Leu Lys Ile Ala Ser Gly Asn Gly Asp 
625                 630                 635                 640 


Leu Val Glu Pro Arg Gln Leu Ser Asn Leu Ser Gln Trp Val Ser Gln 
                645                 650                 655     


Met Pro Phe Pro Met 
            660     


<210>  31
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(777)

<400>  31
atg gag gtg gtg aat gaa ata gtc tca att ggg cag gaa gtt tta ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aaa gtt gat tat gcc caa ctc tgg agt gat gcc agt cac tgt gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctt tac ttg tcc atc gca ttt gtc atc ttg aag ttc act ctt ggc ccc        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctt ggt cca aaa ggt cag tct cgt atg aag ttt gtt ttc acc aat tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctt ctc atg tcc att tat tcg ttg gga tca ttc ctc tca atg gca        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tat gcc atg tac acc atc ggt gtt atg tct gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttt gac aac aac gtc ttc agg atc acc acg cag ttg ttc tat ttg agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctg gag tat att gac tcc ttc tat ttg cca ctg atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg caa ttc ttc cat cat ttg ggg gca ccg atg gat        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tat aat tac cga aat gaa gct gtt tgg att ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ttg aat ggt ttc atc cac tgg atc atg tac ggt tat tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

aga ttg atc aag ctg aag ttc ccc atg cca aaa tcc ctg att aca tca        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att caa ttc aat gtt ggt ttc tac att gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

agg aac att ccc tgt tat cgc caa gat ggg atg agg atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttc ttc aat tac ttt tat gtt ggc aca gtc ttg tgt ttg ttc ttg aat        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tat gtg caa acg tat atc gtc agg aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  32
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  32

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  33
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,794,701
<311>  2008-04-15
<312>  2010-09-14
<313>  (1)..(774)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2008/128241
<311>  2008-04-16
<312>  2008-10-23
<313>  (1)..(774)

<400>  33
atg gaa gca gcc aaa gaa ttg gtt tcc atc gtc caa gag gag ctc ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tat gcc cag ctt tgg cag gat gcc agc agc tgt gag gtc         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctt tac ctc tcg gtg gca ttc gtg gcg atc aag ttc atg ctg cgc cca        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctg aag cgc cag gcc acc ttg aag aag ctg ttc aca gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttc ctc atg tcg atc tat tcc ttt ggc tcc ttc ctg gcc atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tat gcc cta tca gta act ggc atc ctc tcc ggc gac tgt gag acg gcg        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aac gat gtg ttc agg atc aca act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gta gag tac atc gac tcc ttc tac ctt ccc ctt atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

cca ctg tcg ttc ctt cag ttc ttc cat cat ttg ggg gcc ccc att gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg cta ttc tac aaa tac cgc aac gaa gga gtc tgg atc ttt gtc        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ttg aat ggg ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cgg ctc atc aag ctg aac ttc ccc atg ccc aag aac ctg atc acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc atc cag ttc aat gtc ggg ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgc aat gtg cca tgc tac cgc cag gat ggg atg cgc atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggg acg gtc ttg ctg ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttt tac gtg cag acg tac atc cgg aag ccg agg aag aac cga ggg aag        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag                                                                774
Lys Glu                                                                   
                                                                          


<210>  34
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  34

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  35
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,794,701
<311>  2008-04-15
<312>  2010-09-14
<313>  (1)..(774)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2008/128241
<311>  2008-04-16
<312>  2008-10-23
<313>  (1)..(774)

<400>  35
atg gag gct gcc aag gag ctg gtc tcc atc gtc cag gag gaa ctt ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tac gcc cag ctc tgg cag gac gcc tcc tct tgc gag gtt         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcg gtc gct ttc gtg gcc atc aag ttc atg ctt cga cct        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctc aag cga caa gcc acc ctc aaa aag ctg ttc acc gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttt ctc atg tcc atc tac tcg ttc ggc tcc ttc ctg gcg atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tac gct ctc tct gtc act ggt att ctt tcc ggc gat tgt gag act gcc        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aat gac gtg ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gtc gag tac atc gac tcc ttc tac ctt ccc ctc atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

ccc ttg tcg ttt ctg cag ttc ttt cac cat ctc gga gct ccc atc gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aag tat cga aac gaa ggc gtc tgg atc ttt gtt        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctc atc aag ctg aac ttc cct atg ccc aag aac ctc att acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg caa att atc cag ttc aac gtc gga ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cga aac gtg ccc tgc tac cgg cag gac ggt atg cga atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggc acg gtg ctg ctt ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtc cag acc tac att cgg aag cct cga aag aac cga ggc aaa        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag                                                                774
Lys Glu                                                                   
                                                                          


<210>  36
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  36

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  37
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(792)

<400>  37
atg gct gcg gtg ata gag gtc gcc aac gag ttt gta gcc atc acg gca         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gaa acg ctc ccc aaa gtt gac tat caa cga cta tgg cga gac att tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

agt tgt gag cta ctg tat ttc tcc att gcc ttc gtg atc ttg aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acg ttg ggc gag ttg agc gac agc gga aaa aag att ttg aga gtg ttg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aat ctc ttc atg tcc gtg ttc tcc ttg gtg tct ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctt tgc atg ggc tat gcc att tat acc gtg ggc cta tac tct aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac agg gct ttc gac aac tcg ttg ttc cgc ttt gca aca aag gtg        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tac agt aag ttt ttg gag tac atc gac tct ttt tat ctt ccg        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag ccg ctg tct ttc ctg caa ttc ttc cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gcc ccc atg gac atg tgg ctc ttt gtc caa tat tct ggg gaa tct att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttt ttg aat ggg ttc att cac ttt gtt atg tac ggg        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tac tgg act cgg ctg atg aag ttc aat ttc cca atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ttg att acc gcg atg cag atc acg cag ttc aac gtt ggt ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aaa gat att ccc tgc tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ttg gcc tgg atc ttc aat tac tgg tat gtt ggg act gtc ttg ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctg ttc att aat ttc ttc gtc aaa tcc tat gtg ttc cca aag ccg aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gca gat aaa aag gtc caa tag                                        792
Thr Ala Asp Lys Lys Val Gln                                               
            260                                                           


<210>  38
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  38

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  39
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(792)

<400>  39
atg gct gcc gtc atc gag gtg gcc aac gag ttc gtc gct atc act gcc         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gag acc ctt ccc aag gtg gac tat cag cga ctc tgg cga gac atc tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

tcc tgc gag ctc ctg tac ttc tcc att gct ttc gtc atc ctc aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acc ctt ggc gag ctc tcg gat tct ggc aaa aag att ctg cga gtg ctg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aac ctc ttc atg tcc gtc ttt tcg ctg gtg tcc ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctc tgt atg ggt tac gcc atc tac acc gtt gga ctg tac tcc aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac aga gct ttc gac aac agc ttg ttc cga ttt gcc acc aag gtc        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tat tcc aag ttt ctg gag tac atc gac tct ttc tac ctt ccc        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag cct ctg tcc ttt ctg cag ttc ttt cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gct cct atg gac atg tgg ctc ttc gtg cag tac tct ggc gaa tcc att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttc ctg aac gga ttc att cac ttt gtc atg tac ggc        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tat tgg aca cgg ctg atg aag ttc aac ttt ccc atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ctc att acc gca atg cag atc acc cag ttc aac gtt ggc ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aag gac att ccc tgt tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ctg gcc tgg atc ttc aac tac tgg tac gtc ggt acc gtt ctt ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctc ttc atc aac ttc ttt gtc aag tcc tac gtg ttt ccc aag cct aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gcc gac aaa aag gtc cag tag                                        792
Thr Ala Asp Lys Lys Val Gln                                               
            260                                                           


<210>  40
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  40

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  41
<211>  1064
<212>  DNA
<213>  Isochrysis galbana (GenBank Accession No. AF390174)


<220>
<221>  CDS
<222>  (2)..(793)
<223>  delta-9 elongase (IgD9e)

<400>  41
g atg gcc ctc gca aac gac gcg gga gag cgc atc tgg gcg gct gtg acc       49
  Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr         
  1               5                   10                  15              

gac ccg gaa atc ctc att ggc acc ttc tcg tac ttg cta ctc aaa ccg         97
Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro           
            20                  25                  30                    

ctg ctc cgc aat tcc ggg ctg gtg gat gag aag aag ggc gca tac agg        145
Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg           
        35                  40                  45                        

acg tcc atg atc tgg tac aac gtt ctg ctg gcg ctc ttc tct gcg ctg        193
Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu           
    50                  55                  60                            

agc ttc tac gtg acg gcg acc gcc ctc ggc tgg gac tat ggt acg ggc        241
Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly           
65                  70                  75                  80            

gcg tgg ctg cgc agg caa acc ggc gac aca ccg cag ccg ctc ttc cag        289
Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln           
                85                  90                  95                

tgc ccg tcc ccg gtt tgg gac tcg aag ctc ttc aca tgg acc gcc aag        337
Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys           
            100                 105                 110                   

gca ttc tat tac tcc aag tac gtg gag tac ctc gac acg gcc tgg ctg        385
Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu           
        115                 120                 125                       

gtg ctc aag ggc aag agg gtc tcc ttt ctc cag gcc ttc cac cac ttt        433
Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe           
    130                 135                 140                           

ggc gcg ccg tgg gat gtg tac ctc ggc att cgg ctg cac aac gag ggc        481
Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly           
145                 150                 155                 160           

gta tgg atc ttc atg ttt ttc aac tcg ttc att cac acc atc atg tac        529
Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr           
                165                 170                 175               

acc tac tac ggc ctc acc gcc gcc ggg tat aag ttc aag gcc aag ccg        577
Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro           
            180                 185                 190                   

ctc atc acc gcg atg cag atc tgc cag ttc gtg ggc ggc ttc ctg ttg        625
Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu           
        195                 200                 205                       

gtc tgg gac tac atc aac gtc ccc tgc ttc aac tcg gac aaa ggg aag        673
Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys           
    210                 215                 220                           

ttg ttc agc tgg gct ttc aac tat gca tac gtc ggc tcg gtc ttc ttg        721
Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu           
225                 230                 235                 240           

ctc ttc tgc cac ttt ttc tac cag gac aac ttg gca acg aag aaa tcg        769
Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser           
                245                 250                 255               

gcc aag gcg ggc aag cag ctc tag gcctcgagcc ggctcgcggg ttcaaggagg       823
Ala Lys Ala Gly Lys Gln Leu                                               
            260                                                           

gcgacacggg ggtgggacgt ttgcatggag atggattgtg gatgtcctta cgccttactc      883

atcaatgtcc tcccatctct cccctctaga ccttctacta gccatctaga agggcagctc      943

agagacggat accgttcccc ctccccttcc ttttcgtctt tgctttgcca ttgtttgttt     1003

gtctctattt tttaaactat tgacgctaac gcgttacgct cgcaaaaaaa aaaaaaaaaa     1063

a                                                                     1064


<210>  42
<211>  263
<212>  PRT
<213>  Isochrysis galbana (GenBank Accession No. AF390174)

<400>  42

Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr 
1               5                   10                  15      


Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro 
            20                  25                  30          


Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg 
        35                  40                  45              


Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu 
    50                  55                  60                  


Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly 
65                  70                  75                  80  


Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln 
                85                  90                  95      


Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys 
            100                 105                 110         


Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu 
        115                 120                 125             


Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe 
    130                 135                 140                 


Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly 
145                 150                 155                 160 


Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr 
                165                 170                 175     


Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu 
        195                 200                 205             


Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys 
    210                 215                 220                 


Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu 
225                 230                 235                 240 


Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser 
                245                 250                 255     


Ala Lys Ala Gly Lys Gln Leu 
            260             


<210>  43
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  43
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  44
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  44

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  45
<211>  1101
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1101)
<223>  cholinephosphate cytidylyltransferase; GenBank Accession No. 
       XM_502978

<400>  45
atg gcc aaa agc aaa cga cgg tcg gag gct gtg gaa gag cac gtg acc         48
Met Ala Lys Ser Lys Arg Arg Ser Glu Ala Val Glu Glu His Val Thr           
1               5                   10                  15                

ggc tcg gac gag ggc ttg acc gat act tcg ggt cac gtg agc cct gcc         96
Gly Ser Asp Glu Gly Leu Thr Asp Thr Ser Gly His Val Ser Pro Ala           
            20                  25                  30                    

gcc aag aag cag aag aac tcg gag att cat ttc acc acc cag gct gcc        144
Ala Lys Lys Gln Lys Asn Ser Glu Ile His Phe Thr Thr Gln Ala Ala           
        35                  40                  45                        

cag cag ttg gat cgg gag cgc aag gag gag tat ctg gac tcg ctg atc        192
Gln Gln Leu Asp Arg Glu Arg Lys Glu Glu Tyr Leu Asp Ser Leu Ile           
    50                  55                  60                            

gac aac aag gac tat ctc aag tac cgt cct cga ggc tgg aag ctc aac        240
Asp Asn Lys Asp Tyr Leu Lys Tyr Arg Pro Arg Gly Trp Lys Leu Asn           
65                  70                  75                  80            

aac ccg cct acc gac cga cct gtg cga atc tac gcc gat gga gtg ttt        288
Asn Pro Pro Thr Asp Arg Pro Val Arg Ile Tyr Ala Asp Gly Val Phe           
                85                  90                  95                

gat ttg ttc cat ctg gga cac atg cgt cag ctg gag cag tcc aag aag        336
Asp Leu Phe His Leu Gly His Met Arg Gln Leu Glu Gln Ser Lys Lys           
            100                 105                 110                   

gcc ttc ccc aac gca gtg ttg att gtg ggc att ccc agc gac aag gag        384
Ala Phe Pro Asn Ala Val Leu Ile Val Gly Ile Pro Ser Asp Lys Glu           
        115                 120                 125                       

acc cac aag cgg aag gga ttg acc gtg ctg agt gac gtc cag cgg tac        432
Thr His Lys Arg Lys Gly Leu Thr Val Leu Ser Asp Val Gln Arg Tyr           
    130                 135                 140                           

gag acg gtg cga cac tgc aag tgg gtg gac gag gtg gtg gag gat gct        480
Glu Thr Val Arg His Cys Lys Trp Val Asp Glu Val Val Glu Asp Ala           
145                 150                 155                 160           

ccc tgg tgt gtc acc atg gac ttt ctg gaa aaa cac aaa atc gac tac        528
Pro Trp Cys Val Thr Met Asp Phe Leu Glu Lys His Lys Ile Asp Tyr           
                165                 170                 175               

gtg gcc cat gac gat ctg ccc tac gct tcc ggc aac gac gat gat atc        576
Val Ala His Asp Asp Leu Pro Tyr Ala Ser Gly Asn Asp Asp Asp Ile           
            180                 185                 190                   

tac aag ccc atc aag gag aag ggc atg ttt ctg gcc acc cag cga acc        624
Tyr Lys Pro Ile Lys Glu Lys Gly Met Phe Leu Ala Thr Gln Arg Thr           
        195                 200                 205                       

gag ggc att tcc acc tcg gac atc atc acc aag att atc cga gac tac        672
Glu Gly Ile Ser Thr Ser Asp Ile Ile Thr Lys Ile Ile Arg Asp Tyr           
    210                 215                 220                           

gac aag tat tta atg cga aac ttt gcc cgg ggt gct aac cga aag gat        720
Asp Lys Tyr Leu Met Arg Asn Phe Ala Arg Gly Ala Asn Arg Lys Asp           
225                 230                 235                 240           

ctc aac gtc tcg tgg ctc aag aag aac gag ctg gac ttc aag cgt cat        768
Leu Asn Val Ser Trp Leu Lys Lys Asn Glu Leu Asp Phe Lys Arg His           
                245                 250                 255               

gtg gcc gag ttc cga aac tcg ttc aag cga aag aag gtc ggt aag gat        816
Val Ala Glu Phe Arg Asn Ser Phe Lys Arg Lys Lys Val Gly Lys Asp           
            260                 265                 270                   

ctc tac ggc gag att cgc ggt ctg ctg cag aat gtg ctc att tgg aac        864
Leu Tyr Gly Glu Ile Arg Gly Leu Leu Gln Asn Val Leu Ile Trp Asn           
        275                 280                 285                       

ggc gac aac tcc ggc act tcc act ccc cag cga aag acg ctg cag acc        912
Gly Asp Asn Ser Gly Thr Ser Thr Pro Gln Arg Lys Thr Leu Gln Thr           
    290                 295                 300                           

aac gcc aag aag atg tac atg aac gtg ctc aag act ctg cag gct cct        960
Asn Ala Lys Lys Met Tyr Met Asn Val Leu Lys Thr Leu Gln Ala Pro           
305                 310                 315                 320           

gac gct gtt gac gtg gac tcc tcg gag aac gtg tct gag aac gtc act       1008
Asp Ala Val Asp Val Asp Ser Ser Glu Asn Val Ser Glu Asn Val Thr           
                325                 330                 335               

gat gag gag gag gaa gac gac gac gag gtt gat gag gac gaa gaa gcc       1056
Asp Glu Glu Glu Glu Asp Asp Asp Glu Val Asp Glu Asp Glu Glu Ala           
            340                 345                 350                   

gac gac gac gac gaa gac gac gaa gac gag gaa gac gac gag tag           1101
Asp Asp Asp Asp Glu Asp Asp Glu Asp Glu Glu Asp Asp Glu                   
        355                 360                 365                       


<210>  46
<211>  366
<212>  PRT
<213>  Yarrowia lipolytica

<400>  46

Met Ala Lys Ser Lys Arg Arg Ser Glu Ala Val Glu Glu His Val Thr 
1               5                   10                  15      


Gly Ser Asp Glu Gly Leu Thr Asp Thr Ser Gly His Val Ser Pro Ala 
            20                  25                  30          


Ala Lys Lys Gln Lys Asn Ser Glu Ile His Phe Thr Thr Gln Ala Ala 
        35                  40                  45              


Gln Gln Leu Asp Arg Glu Arg Lys Glu Glu Tyr Leu Asp Ser Leu Ile 
    50                  55                  60                  


Asp Asn Lys Asp Tyr Leu Lys Tyr Arg Pro Arg Gly Trp Lys Leu Asn 
65                  70                  75                  80  


Asn Pro Pro Thr Asp Arg Pro Val Arg Ile Tyr Ala Asp Gly Val Phe 
                85                  90                  95      


Asp Leu Phe His Leu Gly His Met Arg Gln Leu Glu Gln Ser Lys Lys 
            100                 105                 110         


Ala Phe Pro Asn Ala Val Leu Ile Val Gly Ile Pro Ser Asp Lys Glu 
        115                 120                 125             


Thr His Lys Arg Lys Gly Leu Thr Val Leu Ser Asp Val Gln Arg Tyr 
    130                 135                 140                 


Glu Thr Val Arg His Cys Lys Trp Val Asp Glu Val Val Glu Asp Ala 
145                 150                 155                 160 


Pro Trp Cys Val Thr Met Asp Phe Leu Glu Lys His Lys Ile Asp Tyr 
                165                 170                 175     


Val Ala His Asp Asp Leu Pro Tyr Ala Ser Gly Asn Asp Asp Asp Ile 
            180                 185                 190         


Tyr Lys Pro Ile Lys Glu Lys Gly Met Phe Leu Ala Thr Gln Arg Thr 
        195                 200                 205             


Glu Gly Ile Ser Thr Ser Asp Ile Ile Thr Lys Ile Ile Arg Asp Tyr 
    210                 215                 220                 


Asp Lys Tyr Leu Met Arg Asn Phe Ala Arg Gly Ala Asn Arg Lys Asp 
225                 230                 235                 240 


Leu Asn Val Ser Trp Leu Lys Lys Asn Glu Leu Asp Phe Lys Arg His 
                245                 250                 255     


Val Ala Glu Phe Arg Asn Ser Phe Lys Arg Lys Lys Val Gly Lys Asp 
            260                 265                 270         


Leu Tyr Gly Glu Ile Arg Gly Leu Leu Gln Asn Val Leu Ile Trp Asn 
        275                 280                 285             


Gly Asp Asn Ser Gly Thr Ser Thr Pro Gln Arg Lys Thr Leu Gln Thr 
    290                 295                 300                 


Asn Ala Lys Lys Met Tyr Met Asn Val Leu Lys Thr Leu Gln Ala Pro 
305                 310                 315                 320 


Asp Ala Val Asp Val Asp Ser Ser Glu Asn Val Ser Glu Asn Val Thr 
                325                 330                 335     


Asp Glu Glu Glu Glu Asp Asp Asp Glu Val Asp Glu Asp Glu Glu Ala 
            340                 345                 350         


Asp Asp Asp Asp Glu Asp Asp Glu Asp Glu Glu Asp Asp Glu 
        355                 360                 365     


<210>  47
<211>  1185
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1185)
<223>  diacylglycerol cholinephosphotransferase (YlCPT1)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  WO 2006/052870
<311>  2005-11-03
<312>  2006-05-18
<313>  (1)..(1185)

<300>
<302>  HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF
<310>  US 2006-0115881-A1
<311>  2005-11-02
<312>  2006-06-01
<313>  (1)..(1185)

<400>  47
atg ggc gta ttc att aaa cag gag cag ctt ccg gct ctc aag aag tac         48
Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr           
1               5                   10                  15                

aag tac tcc gcc gag gat cac tcg ttc atc tcc aac aac att ctg cgc         96
Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg           
            20                  25                  30                    

ccc ttc tgg cga cag ttt gtc aaa atc ttc cct ctg tgg atg gcc ccc        144
Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro           
        35                  40                  45                        

aac atg gtg act ctg ttg ggc ttc ttc ttt gtc att gtg aac ttc atc        192
Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile           
    50                  55                  60                            

acc atg ctc att gtt gat ccc acc cac gac cgc gag cct ccc aga tgg        240
Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp           
65                  70                  75                  80            

gtc tac ctc acc tac gct ctg ggt ctg ttc ctt tac cag aca ttt gat        288
Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp           
                85                  90                  95                

gcc tgt gac gga tcc cat gcc cga cga act ggc cag agt gga ccc ctt        336
Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu           
            100                 105                 110                   

gga gag ctg ttt gac cac tgt gtc gac gcc atg aat acc tct ctg att        384
Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile           
        115                 120                 125                       

ctc acg gtg gtg gtg tcc acc acc cat atg gga tat aac atg aag ctg        432
Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu           
    130                 135                 140                           

ctg att gtg cag att gcc gct ctc gga aac ttc tac ctg tcg acc tgg        480
Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp           
145                 150                 155                 160           

gag acc tac cat acc gga act ctg tac ctt tct ggc ttc tct ggt cct        528
Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro           
                165                 170                 175               

gtt gaa ggt atc ttg att ctg gtg gct ctt ttc gtc ctc acc ttc ttc        576
Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe           
            180                 185                 190                   

act ggt ccc aac gtg tac gct ctg acc gtc tac gag gct ctt ccc gaa        624
Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu           
        195                 200                 205                       

tcc atc act tcg ctg ctg cct gcc agc ttc ctg gac gtc acc atc acc        672
Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr           
    210                 215                 220                           

cag atc tac att gga ttc gga gtg ctg ggc atg gtg ttc aac atc tac        720
Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr           
225                 230                 235                 240           

ggc gcc tgc gga aac gtg atc aag tac tac aac aac aag ggc aag agc        768
Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser           
                245                 250                 255               

gct ctc ccc gcc att ctc gga atc gcc ccc ttt ggc atc ttc tac gtc        816
Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val           
            260                 265                 270                   

ggc gtc ttt gcc tgg gcc cat gtt gct cct ctg ctt ctc tcc aag tac        864
Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr           
        275                 280                 285                       

gcc atc gtc tat ctg ttt gcc att ggg gct gcc ttt gcc atg caa gtc        912
Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val           
    290                 295                 300                           

ggc cag atg att ctt gcc cat ctc gtg ctt gct ccc ttc ccc cac tgg        960
Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp           
305                 310                 315                 320           

aac gtg ctg ctc ttc ttc ccc ttt gtg gga ctg gca gtg cac tac att       1008
Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile           
                325                 330                 335               

gca ccc gtg ttt ggc tgg gac gcc gat atc gtg tcg gtt aac act ctc       1056
Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu           
            340                 345                 350                   

ttc acc tgt ttt ggc gcc acc ctc tcc att tac gcc ttc ttt gtg ctt       1104
Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu           
        355                 360                 365                       

gag atc atc gac gag atc acc aac tac ctc gat atc tgg tgt ctg cga       1152
Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg           
    370                 375                 380                           

atc aag tac cct cag gag aag aag act gag taa                           1185
Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu                                   
385                 390                                                   


<210>  48
<211>  394
<212>  PRT
<213>  Yarrowia lipolytica

<400>  48

Met Gly Val Phe Ile Lys Gln Glu Gln Leu Pro Ala Leu Lys Lys Tyr 
1               5                   10                  15      


Lys Tyr Ser Ala Glu Asp His Ser Phe Ile Ser Asn Asn Ile Leu Arg 
            20                  25                  30          


Pro Phe Trp Arg Gln Phe Val Lys Ile Phe Pro Leu Trp Met Ala Pro 
        35                  40                  45              


Asn Met Val Thr Leu Leu Gly Phe Phe Phe Val Ile Val Asn Phe Ile 
    50                  55                  60                  


Thr Met Leu Ile Val Asp Pro Thr His Asp Arg Glu Pro Pro Arg Trp 
65                  70                  75                  80  


Val Tyr Leu Thr Tyr Ala Leu Gly Leu Phe Leu Tyr Gln Thr Phe Asp 
                85                  90                  95      


Ala Cys Asp Gly Ser His Ala Arg Arg Thr Gly Gln Ser Gly Pro Leu 
            100                 105                 110         


Gly Glu Leu Phe Asp His Cys Val Asp Ala Met Asn Thr Ser Leu Ile 
        115                 120                 125             


Leu Thr Val Val Val Ser Thr Thr His Met Gly Tyr Asn Met Lys Leu 
    130                 135                 140                 


Leu Ile Val Gln Ile Ala Ala Leu Gly Asn Phe Tyr Leu Ser Thr Trp 
145                 150                 155                 160 


Glu Thr Tyr His Thr Gly Thr Leu Tyr Leu Ser Gly Phe Ser Gly Pro 
                165                 170                 175     


Val Glu Gly Ile Leu Ile Leu Val Ala Leu Phe Val Leu Thr Phe Phe 
            180                 185                 190         


Thr Gly Pro Asn Val Tyr Ala Leu Thr Val Tyr Glu Ala Leu Pro Glu 
        195                 200                 205             


Ser Ile Thr Ser Leu Leu Pro Ala Ser Phe Leu Asp Val Thr Ile Thr 
    210                 215                 220                 


Gln Ile Tyr Ile Gly Phe Gly Val Leu Gly Met Val Phe Asn Ile Tyr 
225                 230                 235                 240 


Gly Ala Cys Gly Asn Val Ile Lys Tyr Tyr Asn Asn Lys Gly Lys Ser 
                245                 250                 255     


Ala Leu Pro Ala Ile Leu Gly Ile Ala Pro Phe Gly Ile Phe Tyr Val 
            260                 265                 270         


Gly Val Phe Ala Trp Ala His Val Ala Pro Leu Leu Leu Ser Lys Tyr 
        275                 280                 285             


Ala Ile Val Tyr Leu Phe Ala Ile Gly Ala Ala Phe Ala Met Gln Val 
    290                 295                 300                 


Gly Gln Met Ile Leu Ala His Leu Val Leu Ala Pro Phe Pro His Trp 
305                 310                 315                 320 


Asn Val Leu Leu Phe Phe Pro Phe Val Gly Leu Ala Val His Tyr Ile 
                325                 330                 335     


Ala Pro Val Phe Gly Trp Asp Ala Asp Ile Val Ser Val Asn Thr Leu 
            340                 345                 350         


Phe Thr Cys Phe Gly Ala Thr Leu Ser Ile Tyr Ala Phe Phe Val Leu 
        355                 360                 365             


Glu Ile Ile Asp Glu Ile Thr Asn Tyr Leu Asp Ile Trp Cys Leu Arg 
    370                 375                 380                 


Ile Lys Tyr Pro Gln Glu Lys Lys Thr Glu 
385                 390                 


<210>  49
<211>  1518
<212>  DNA
<213>  Rhizobium leguminosarum bv. viciae 3841


<220>
<221>  CDS
<222>  (1)..(1518)
<223>  synthetic malonyl-CoA synthetase (codon-optimized for Yarrowia 
       lipolytica)

<400>  49
atg gtc tcc aac cac ctg ttc gac gcc atg cga gct gcc gct ccc gga         48
Met Val Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly           
1               5                   10                  15                

gac gca cct ttc att cga atc gac aac gct cgg acc tgg act tac gat         96
Asp Ala Pro Phe Ile Arg Ile Asp Asn Ala Arg Thr Trp Thr Tyr Asp           
            20                  25                  30                    

gac gcc att gct ctt tcc ggt cga atc gct gga gct atg gac gca ctc        144
Asp Ala Ile Ala Leu Ser Gly Arg Ile Ala Gly Ala Met Asp Ala Leu           
        35                  40                  45                        

ggc att cga ccc gga gac aga gtt gcc gtg cag gtc gag aag tct gcc        192
Gly Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala           
    50                  55                  60                            

gag gcg ttg att ctc tac ctg gcc tgt ctt cga acc gga gct gtc tac        240
Glu Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Thr Gly Ala Val Tyr           
65                  70                  75                  80            

ctg cct ctc aac act gcc tac acc ctg gcc gag ctc gac tac ttc atc        288
Leu Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile           
                85                  90                  95                

ggc gat gcc gaa ccg cgt ctg gtg gtc gtt gct ccc gca gct cga ggt        336
Gly Asp Ala Glu Pro Arg Leu Val Val Val Ala Pro Ala Ala Arg Gly           
            100                 105                 110                   

ggc gtg gag aca att gcc aag cga cac ggt gct atc gtc gaa acc ctc        384
Gly Val Glu Thr Ile Ala Lys Arg His Gly Ala Ile Val Glu Thr Leu           
        115                 120                 125                       

gac gcc gat gga cga ggc tcc ttg ctg gac ctt gct aga gat gag cct        432
Asp Ala Asp Gly Arg Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro           
    130                 135                 140                           

gcc gac ttt gtc gat gct tcg cga tct gcc gac gat ctg gct gct att        480
Ala Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile           
145                 150                 155                 160           

ctc tac act tcc ggt aca acc gga cga tcg aag ggt gcc atg ctt act        528
Leu Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr           
                165                 170                 175               

cat ggc aat ctg ctc tcc aac gct ctc acc ttg cga gac tat tgg aga        576
His Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Tyr Trp Arg           
            180                 185                 190                   

gtt acc gca gac gat cga ctc atc cat gcc ttg cca atc ttt cac act        624
Val Thr Ala Asp Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr           
        195                 200                 205                       

cat ggt ctg ttc gtt gct acg aac gtc aca ctg ctt gca gga gcc tcg        672
His Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser           
    210                 215                 220                           

atg ttt ctg ctc tcc aag ttc gat gcc gac gag gtc gtt tct ctc atg        720
Met Phe Leu Leu Ser Lys Phe Asp Ala Asp Glu Val Val Ser Leu Met           
225                 230                 235                 240           

cca cag gcc acc atg ctt atg ggc gtg ccc aca ttc tac gtt cga ttg        768
Pro Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu           
                245                 250                 255               

ctg cag agt cct cga ctc gag aag ggt gct gtg gcc agc atc aga ctg        816
Leu Gln Ser Pro Arg Leu Glu Lys Gly Ala Val Ala Ser Ile Arg Leu           
            260                 265                 270                   

ttc att tct gga tca gct ccc ttg ctt gcc gaa acc cac gcc gag ttt        864
Phe Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Ala Glu Phe           
        275                 280                 285                       

cat gct cgt act ggt cac gcc att ctc gag cga tac ggc atg acg gaa        912
His Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu           
    290                 295                 300                           

acc aac atg aat act tcc aac ccc tac gag ggc aag cgt att gcc gga        960
Thr Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly           
305                 310                 315                 320           

acc gtt ggt ttt cct ctg ccc gac gtc act gtg cga gtc acc gat ccc       1008
Thr Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro           
                325                 330                 335               

gcc acc ggt ctc gtt ctt cca cct gaa gag act ggc atg atc gag atc       1056
Ala Thr Gly Leu Val Leu Pro Pro Glu Glu Thr Gly Met Ile Glu Ile           
            340                 345                 350                   

aag gga ccc aac gtc ttc aag ggc tat tgg cga atg ccc gaa aag acc       1104
Lys Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr           
        355                 360                 365                       

gct gcc gag ttt acc gca gac ggt ttc ttt atc tct gga gat ctc ggc       1152
Ala Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly           
    370                 375                 380                           

aag atc gac cga gaa ggt tac gtt cac att gtg gga cga ggc aag gac       1200
Lys Ile Asp Arg Glu Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp           
385                 390                 395                 400           

ctg gtc att tcc ggt ggc tac aac atc tat ccc aaa gag gtc gaa ggc       1248
Leu Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly           
                405                 410                 415               

gag atc gac cag atc gag ggt gtg gtc gag tct gct gtc att ggt gtt       1296
Glu Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val           
            420                 425                 430                   

cct cat ccc gat ttc gga gaa ggt gtc acc gct gtt gtc gtg tgc aaa       1344
Pro His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Cys Lys           
        435                 440                 445                       

cct ggt gcc gtt ctc gac gaa aag acc atc gtg tct gct ctg cag gac       1392
Pro Gly Ala Val Leu Asp Glu Lys Thr Ile Val Ser Ala Leu Gln Asp           
    450                 455                 460                           

cgt ctt gcc cga tac aag caa ccc aag cgg att atc ttt gcc gac gat       1440
Arg Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Asp Asp           
465                 470                 475                 480           

ctg cct cga aac act atg gga aag gtt cag aag aac att ctt cga cag       1488
Leu Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln           
                485                 490                 495               

caa tac gcc gat ctc tac acc aga cga taa                               1518
Gln Tyr Ala Asp Leu Tyr Thr Arg Arg                                       
            500                 505                                       


<210>  50
<211>  505
<212>  PRT
<213>  Rhizobium leguminosarum bv. viciae 3841

<400>  50

Met Val Ser Asn His Leu Phe Asp Ala Met Arg Ala Ala Ala Pro Gly 
1               5                   10                  15      


Asp Ala Pro Phe Ile Arg Ile Asp Asn Ala Arg Thr Trp Thr Tyr Asp 
            20                  25                  30          


Asp Ala Ile Ala Leu Ser Gly Arg Ile Ala Gly Ala Met Asp Ala Leu 
        35                  40                  45              


Gly Ile Arg Pro Gly Asp Arg Val Ala Val Gln Val Glu Lys Ser Ala 
    50                  55                  60                  


Glu Ala Leu Ile Leu Tyr Leu Ala Cys Leu Arg Thr Gly Ala Val Tyr 
65                  70                  75                  80  


Leu Pro Leu Asn Thr Ala Tyr Thr Leu Ala Glu Leu Asp Tyr Phe Ile 
                85                  90                  95      


Gly Asp Ala Glu Pro Arg Leu Val Val Val Ala Pro Ala Ala Arg Gly 
            100                 105                 110         


Gly Val Glu Thr Ile Ala Lys Arg His Gly Ala Ile Val Glu Thr Leu 
        115                 120                 125             


Asp Ala Asp Gly Arg Gly Ser Leu Leu Asp Leu Ala Arg Asp Glu Pro 
    130                 135                 140                 


Ala Asp Phe Val Asp Ala Ser Arg Ser Ala Asp Asp Leu Ala Ala Ile 
145                 150                 155                 160 


Leu Tyr Thr Ser Gly Thr Thr Gly Arg Ser Lys Gly Ala Met Leu Thr 
                165                 170                 175     


His Gly Asn Leu Leu Ser Asn Ala Leu Thr Leu Arg Asp Tyr Trp Arg 
            180                 185                 190         


Val Thr Ala Asp Asp Arg Leu Ile His Ala Leu Pro Ile Phe His Thr 
        195                 200                 205             


His Gly Leu Phe Val Ala Thr Asn Val Thr Leu Leu Ala Gly Ala Ser 
    210                 215                 220                 


Met Phe Leu Leu Ser Lys Phe Asp Ala Asp Glu Val Val Ser Leu Met 
225                 230                 235                 240 


Pro Gln Ala Thr Met Leu Met Gly Val Pro Thr Phe Tyr Val Arg Leu 
                245                 250                 255     


Leu Gln Ser Pro Arg Leu Glu Lys Gly Ala Val Ala Ser Ile Arg Leu 
            260                 265                 270         


Phe Ile Ser Gly Ser Ala Pro Leu Leu Ala Glu Thr His Ala Glu Phe 
        275                 280                 285             


His Ala Arg Thr Gly His Ala Ile Leu Glu Arg Tyr Gly Met Thr Glu 
    290                 295                 300                 


Thr Asn Met Asn Thr Ser Asn Pro Tyr Glu Gly Lys Arg Ile Ala Gly 
305                 310                 315                 320 


Thr Val Gly Phe Pro Leu Pro Asp Val Thr Val Arg Val Thr Asp Pro 
                325                 330                 335     


Ala Thr Gly Leu Val Leu Pro Pro Glu Glu Thr Gly Met Ile Glu Ile 
            340                 345                 350         


Lys Gly Pro Asn Val Phe Lys Gly Tyr Trp Arg Met Pro Glu Lys Thr 
        355                 360                 365             


Ala Ala Glu Phe Thr Ala Asp Gly Phe Phe Ile Ser Gly Asp Leu Gly 
    370                 375                 380                 


Lys Ile Asp Arg Glu Gly Tyr Val His Ile Val Gly Arg Gly Lys Asp 
385                 390                 395                 400 


Leu Val Ile Ser Gly Gly Tyr Asn Ile Tyr Pro Lys Glu Val Glu Gly 
                405                 410                 415     


Glu Ile Asp Gln Ile Glu Gly Val Val Glu Ser Ala Val Ile Gly Val 
            420                 425                 430         


Pro His Pro Asp Phe Gly Glu Gly Val Thr Ala Val Val Val Cys Lys 
        435                 440                 445             


Pro Gly Ala Val Leu Asp Glu Lys Thr Ile Val Ser Ala Leu Gln Asp 
    450                 455                 460                 


Arg Leu Ala Arg Tyr Lys Gln Pro Lys Arg Ile Ile Phe Ala Asp Asp 
465                 470                 475                 480 


Leu Pro Arg Asn Thr Met Gly Lys Val Gln Lys Asn Ile Leu Arg Gln 
                485                 490                 495     


Gln Tyr Ala Asp Leu Tyr Thr Arg Arg 
            500                 505 


<210>  51
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex1p;  GenBank Accession No. CAG82178

<400>  51

Met Thr Ser Lys Ser Asp Tyr Ser Gly Lys Asp Lys Ile Glu Leu Asp 
1               5                   10                  15      


Pro Val Phe Ala Lys Ser Ile Asp Leu Leu Pro Asn Thr Gln Val Val 
            20                  25                  30          


Ile Asp Ile Gln Leu Asn Pro Lys Ile Ala His Thr Ile His Leu Glu 
        35                  40                  45              


Pro Val Thr Val Ala Asp Trp Glu Ile Val Glu Leu His Ala Ala Tyr 
    50                  55                  60                  


Leu Glu Ser Arg Met Ile Asn Gln Val Arg Ala Val Ser Pro Asn Gln 
65                  70                  75                  80  


Pro Val Thr Val Tyr Pro Ser Ser Thr Thr Ser Ala Thr Leu Lys Val 
                85                  90                  95      


Ile Arg Ile Glu Pro Asp Leu Gly Ala Ala Gly Phe Ala Lys Leu Ser 
            100                 105                 110         


Pro Asp Ser Glu Val Val Val Ala Pro Lys Gln Arg Lys Lys Glu Glu 
        115                 120                 125             


Lys Gln Val Lys Lys Arg Ser Gly Ser Ala Arg Ser Thr Gly Ser Gln 
    130                 135                 140                 


Lys Arg Lys Gly Gly Arg Gly Pro His Ala Leu Arg Arg Ala Ile Ser 
145                 150                 155                 160 


Glu Asp Phe Asp Gly His Leu Arg Leu Glu Val Ser Leu Asp Val Ser 
                165                 170                 175     


Gln Leu Pro Pro Glu Phe His Gln Leu Lys Asn Val Ser Ile Lys Val 
            180                 185                 190         


Ile Thr Pro Pro Asn Leu Ala Ser Pro Gln Gln Ala Ala Ser Ile Ala 
        195                 200                 205             


Val Glu Glu Lys Ser Glu Glu Ser Leu Ser Gln Asn Lys Pro Pro Ser 
    210                 215                 220                 


Ser Glu Pro Lys Val Glu Val Pro Pro Asp Ile Ile Asn Pro Ala Ser 
225                 230                 235                 240 


Glu Ile Val Ala Thr Leu Val Asn Asp Thr Thr Ser Pro Thr Gly His 
                245                 250                 255     


Ala Lys Leu Ser Tyr Ala Leu Ala Asp Ala Leu Gly Ile Pro Ser Ser 
            260                 265                 270         


Val Gly His Val Ile Arg Phe Glu Ser Ala Ser Lys Pro Leu Ser Gln 
        275                 280                 285             


Lys Pro Gly Ala Leu Val Ile His Arg Phe Ile Thr Lys Thr Val Gly 
    290                 295                 300                 


Ala Ala Glu Gln Lys Ser Leu Arg Leu Lys Gly Glu Lys Asn Ala Asp 
305                 310                 315                 320 


Asp Gly Val Ser Ala Asp Asp Gln Phe Ser Leu Leu Glu Glu Leu Lys 
                325                 330                 335     


Lys Leu Gln Met Leu Glu Gly Pro Ile Thr Asn Phe Gln Arg Leu Pro 
            340                 345                 350         


Pro Ile Pro Glu Leu Leu Pro Leu Gly Gly Val Ile Gly Leu Gln Asn 
        355                 360                 365             


Ser Glu Gly Trp Ile Gln Gly Gly Tyr Leu Gly Glu Glu Pro Ile Pro 
    370                 375                 380                 


Phe Val Ser Gly Ser Glu Ile Leu Arg Ser Glu Ser Ser Leu Ser Pro 
385                 390                 395                 400 


Ser Asn Ile Glu Ser Glu Asp Lys Arg Val Val Gly Leu Asp Asn Met 
                405                 410                 415     


Leu Asn Lys Ile Asn Glu Val Leu Ser Arg Asp Ser Ile Gly Cys Leu 
            420                 425                 430         


Val Tyr Gly Ser Arg Gly Ser Gly Lys Ser Ala Val Leu Asn His Ile 
        435                 440                 445             


Lys Lys Glu Cys Lys Val Ser His Thr His Thr Val Ser Ile Ala Cys 
    450                 455                 460                 


Gly Leu Ile Ala Gln Asp Arg Val Gln Ala Val Arg Glu Ile Leu Thr 
465                 470                 475                 480 


Lys Ala Phe Leu Glu Ala Ser Trp Phe Ser Pro Ser Val Leu Phe Leu 
                485                 490                 495     


Asp Asp Ile Asp Ala Leu Met Pro Ala Glu Val Glu His Ala Asp Ser 
            500                 505                 510         


Ser Arg Thr Arg Gln Leu Thr Gln Leu Phe Leu Glu Leu Ala Leu Pro 
        515                 520                 525             


Ile Met Lys Ser Arg His Val Ser Val Val Ala Ser Ala Gln Ala Lys 
    530                 535                 540                 


Glu Ser Leu His Met Asn Leu Val Thr Gly His Val Phe Glu Glu Leu 
545                 550                 555                 560 


Phe His Leu Lys Ser Pro Asp Lys Glu Ala Arg Leu Ala Ile Leu Ser 
                565                 570                 575     


Glu Ala Val Lys Leu Met Asp Gln Asn Val Ser Phe Ser Gln Asn Asp 
            580                 585                 590         


Val Leu Glu Ile Ala Ser Gln Val Asp Gly Tyr Leu Pro Gly Asp Leu 
        595                 600                 605             


Trp Thr Leu Ser Glu Arg Ala Gln His Glu Met Ala Leu Arg Gln Ile 
    610                 615                 620                 


Glu Ile Gly Leu Glu Asn Pro Ser Ile Gln Leu Ala Asp Phe Met Lys 
625                 630                 635                 640 


Ala Leu Glu Asp Phe Val Pro Ser Ser Leu Arg Gly Val Lys Leu Gln 
                645                 650                 655     


Lys Ser Asn Val Lys Trp Asn Asp Ile Gly Gly Leu Lys Glu Thr Lys 
            660                 665                 670         


Ala Val Leu Leu Glu Thr Leu Glu Trp Pro Thr Lys Tyr Ala Pro Ile 
        675                 680                 685             


Phe Ala Ser Cys Pro Leu Arg Leu Arg Ser Gly Leu Leu Leu Tyr Gly 
    690                 695                 700                 


Tyr Pro Gly Cys Gly Lys Thr Tyr Leu Ala Ser Ala Val Ala Ala Gln 
705                 710                 715                 720 


Cys Gly Leu Asn Phe Ile Ser Ile Lys Gly Pro Glu Ile Leu Asn Lys 
                725                 730                 735     


Tyr Ile Gly Ala Ser Glu Gln Ser Val Arg Glu Leu Phe Glu Arg Ala 
            740                 745                 750         


Gln Ala Ala Lys Pro Cys Ile Leu Phe Phe Asp Glu Phe Asp Ser Ile 
        755                 760                 765             


Ala Pro Lys Arg Gly His Asp Ser Thr Gly Val Thr Asp Arg Val Val 
    770                 775                 780                 


Asn Gln Met Leu Thr Gln Met Asp Gly Ala Glu Gly Leu Asp Gly Val 
785                 790                 795                 800 


Tyr Val Leu Ala Ala Thr Ser Arg Pro Asp Leu Ile Asp Pro Ala Leu 
                805                 810                 815     


Leu Arg Pro Gly Arg Leu Asp Lys Met Leu Ile Cys Asp Leu Pro Ser 
            820                 825                 830         


Tyr Glu Asp Arg Leu Asp Ile Leu Arg Ala Ile Val Asp Gly Lys Met 
        835                 840                 845             


His Leu Asp Gly Glu Val Glu Leu Glu Tyr Val Ala Ser Arg Thr Asp 
    850                 855                 860                 


Gly Phe Ser Gly Ala Asp Leu Gln Ala Val Met Phe Asn Ala Tyr Leu 
865                 870                 875                 880 


Glu Ala Ile His Glu Val Val Asp Val Ala Asp Asp Thr Ala Ala Asp 
                885                 890                 895     


Thr Pro Ala Leu Glu Asp Lys Arg Leu Glu Phe Phe Gln Thr Thr Leu 
            900                 905                 910         


Gly Asp Ala Lys Lys Asp Pro Ala Ala Val Gln Asn Glu Val Met Asn 
        915                 920                 925             


Ala Arg Ala Ala Val Ala Glu Lys Ala Arg Val Thr Ala Lys Leu Glu 
    930                 935                 940                 


Ala Leu Phe Lys Gly Met Ser Val Gly Val Asp Asn Asp Asp Asp Lys 
945                 950                 955                 960 


Pro Arg Lys Lys Ala Val Val Val Ile Lys Pro Gln His Met Asn Lys 
                965                 970                 975     


Ser Leu Asp Glu Thr Ser Pro Ser Ile Ser Lys Lys Glu Leu Leu Lys 
            980                 985                 990         


Leu Lys Gly Ile Tyr Ser Gln Phe  Val Ser Gly Arg Ser  Gly Asp Met 
        995                 1000                 1005             


Pro Pro  Gly Thr Ala Ser Thr  Asp Val Gly Gly Arg  Ala Thr Leu 
    1010                 1015                 1020             


Ala 
    


<210>  52
<211>  381
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(381)
<223>  YlPex2p; GenBank Accession No. CAG77647

<400>  52

Met Ser Ser Val Leu Arg Leu Phe Lys Ile Gly Ala Pro Val Pro Asn 
1               5                   10                  15      


Val Arg Val His Gln Leu Asp Ala Ser Leu Leu Asp Ala Glu Leu Val 
            20                  25                  30          


Asp Leu Leu Lys Asn Gln Leu Phe Lys Gly Phe Thr Asn Phe His Pro 
        35                  40                  45              


Glu Phe Arg Asp Lys Tyr Glu Ser Glu Leu Val Leu Ala Leu Lys Leu 
    50                  55                  60                  


Ile Leu Phe Lys Leu Thr Val Trp Asp His Ala Ile Thr Tyr Gly Gly 
65                  70                  75                  80  


Lys Leu Gln Asn Leu Lys Phe Ile Asp Ser Arg His Ser Ser Lys Leu 
                85                  90                  95      


Gln Ile Gln Pro Ser Val Ile Gln Lys Leu Gly Tyr Gly Ile Leu Val 
            100                 105                 110         


Val Gly Gly Gly Tyr Leu Trp Ser Lys Ile Glu Gly Tyr Leu Leu Ala 
        115                 120                 125             


Arg Ser Glu Asp Asp Val Ala Thr Asp Gly Thr Ser Val Arg Gly Ala 
    130                 135                 140                 


Ser Ala Ala Arg Gly Ala Leu Lys Val Ala Asn Phe Ala Ser Leu Leu 
145                 150                 155                 160 


Tyr Ser Ala Ala Thr Leu Gly Asn Phe Val Ala Phe Leu Tyr Thr Gly 
                165                 170                 175     


Arg Tyr Ala Thr Val Ile Met Arg Leu Leu Arg Ile Arg Leu Val Pro 
            180                 185                 190         


Ser Gln Arg Thr Ser Ser Arg Gln Val Ser Tyr Glu Phe Gln Asn Arg 
        195                 200                 205             


Gln Leu Val Trp Asn Ala Phe Thr Glu Phe Leu Ile Phe Ile Leu Pro 
    210                 215                 220                 


Leu Leu Gln Leu Pro Lys Leu Lys Arg Arg Ile Glu Arg Lys Leu Gln 
225                 230                 235                 240 


Ser Leu Asn Val Thr Arg Val Gly Asn Val Glu Glu Ala Ser Glu Gly 
                245                 250                 255     


Glu Leu Ala His Leu Pro Gln Lys Thr Cys Ala Ile Cys Phe Arg Asp 
            260                 265                 270         


Glu Glu Glu Gln Glu Gly Gly Gly Gly Ala Ser His Tyr Ser Thr Asp 
        275                 280                 285             


Val Thr Asn Pro Tyr Gln Ala Asp Cys Gly His Val Tyr Cys Tyr Val 
    290                 295                 300                 


Cys Leu Val Thr Lys Leu Ala Gln Gly Asp Gly Asp Gly Trp Asn Cys 
305                 310                 315                 320 


Tyr Arg Cys Ala Lys Gln Val Gln Lys Met Lys Pro Trp Val Asp Val 
                325                 330                 335     


Asp Glu Ala Ala Val Val Gly Ala Ala Glu Met His Glu Lys Val Asp 
            340                 345                 350         


Val Ile Glu His Ala Glu Asp Asn Glu Gln Glu Glu Glu Glu Phe Asp 
        355                 360                 365             


Asp Asp Asp Glu Asp Ser Asn Phe Gln Leu Met Lys Asp 
    370                 375                 380     


<210>  53
<211>  431
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(431)
<223>  YlPex3p; GenBank Accession No. CAG78565

<400>  53

Met Asp Phe Phe Arg Arg His Gln Lys Lys Val Leu Ala Leu Val Gly 
1               5                   10                  15      


Val Ala Leu Ser Ser Tyr Leu Phe Ile Asp Tyr Val Lys Lys Lys Phe 
            20                  25                  30          


Phe Glu Ile Gln Gly Arg Leu Ser Ser Glu Arg Thr Ala Lys Gln Asn 
        35                  40                  45              


Leu Arg Arg Arg Phe Glu Gln Asn Gln Gln Asp Ala Asp Phe Thr Ile 
    50                  55                  60                  


Met Ala Leu Leu Ser Ser Leu Thr Thr Pro Val Met Glu Arg Tyr Pro 
65                  70                  75                  80  


Val Asp Gln Ile Lys Ala Glu Leu Gln Ser Lys Arg Arg Pro Thr Asp 
                85                  90                  95      


Arg Val Leu Ala Leu Glu Ser Ser Thr Ser Ser Ser Ala Thr Ala Gln 
            100                 105                 110         


Thr Val Pro Thr Met Thr Ser Gly Ala Thr Glu Glu Gly Glu Lys Ser 
        115                 120                 125             


Lys Thr Gln Leu Trp Gln Asp Leu Lys Arg Thr Thr Ile Ser Arg Ala 
    130                 135                 140                 


Phe Ser Leu Val Tyr Ala Asp Ala Leu Leu Ile Phe Phe Thr Arg Leu 
145                 150                 155                 160 


Gln Leu Asn Ile Leu Gly Arg Arg Asn Tyr Val Asn Ser Val Val Ala 
                165                 170                 175     


Leu Ala Gln Gln Gly Arg Glu Gly Asn Ala Glu Gly Arg Val Ala Pro 
            180                 185                 190         


Ser Phe Gly Asp Leu Ala Asp Met Gly Tyr Phe Gly Asp Leu Ser Gly 
        195                 200                 205             


Ser Ser Ser Phe Gly Glu Thr Ile Val Asp Pro Asp Leu Asp Glu Gln 
    210                 215                 220                 


Tyr Leu Thr Phe Ser Trp Trp Leu Leu Asn Glu Gly Trp Val Ser Leu 
225                 230                 235                 240 


Ser Glu Arg Val Glu Glu Ala Val Arg Arg Val Trp Asp Pro Val Ser 
                245                 250                 255     


Pro Lys Ala Glu Leu Gly Phe Asp Glu Leu Ser Glu Leu Ile Gly Arg 
            260                 265                 270         


Thr Gln Met Leu Ile Asp Arg Pro Leu Asn Pro Ser Ser Pro Leu Asn 
        275                 280                 285             


Phe Leu Ser Gln Leu Leu Pro Pro Arg Glu Gln Glu Glu Tyr Val Leu 
    290                 295                 300                 


Ala Gln Asn Pro Ser Asp Thr Ala Ala Pro Ile Val Gly Pro Thr Leu 
305                 310                 315                 320 


Arg Arg Leu Leu Asp Glu Thr Ala Asp Phe Ile Glu Ser Pro Asn Ala 
                325                 330                 335     


Ala Glu Val Ile Glu Arg Leu Val His Ser Gly Leu Ser Val Phe Met 
            340                 345                 350         


Asp Lys Leu Ala Val Thr Phe Gly Ala Thr Pro Ala Asp Ser Gly Ser 
        355                 360                 365             


Pro Tyr Pro Val Val Leu Pro Thr Ala Lys Val Lys Leu Pro Ser Ile 
    370                 375                 380                 


Leu Ala Asn Met Ala Arg Gln Ala Gly Gly Met Ala Gln Gly Ser Pro 
385                 390                 395                 400 


Gly Val Glu Asn Glu Tyr Ile Asp Val Met Asn Gln Val Gln Glu Leu 
                405                 410                 415     


Thr Ser Phe Ser Ala Val Val Tyr Ser Ser Phe Asp Trp Ala Leu 
            420                 425                 430     


<210>  54
<211>  395
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(395)
<223>  YlPex3Bp; GenBank Accession No. CAG83356

<400>  54

Met Leu Gln Ser Leu Asn Arg Asn Lys Lys Arg Leu Ala Val Ser Thr 
1               5                   10                  15      


Gly Leu Ile Ala Val Ala Tyr Val Val Ile Ser Tyr Thr Thr Lys Arg 
            20                  25                  30          


Leu Ile Glu Lys Gln Glu Gln Lys Leu Glu Glu Glu Arg Ala Lys Glu 
        35                  40                  45              


Arg Leu Lys Gln Leu Phe Ala Gln Thr Gln Asn Glu Ala Ala Phe His 
    50                  55                  60                  


Thr Ala Ser Val Leu Pro Gln Leu Cys Glu Gln Ile Met Glu Phe Val 
65                  70                  75                  80  


Ala Val Glu Lys Ile Ala Glu Gln Leu Gln Asn Met Arg Ala Glu Lys 
                85                  90                  95      


Arg Lys Lys Gln Asn Met Asp Asp Asp Lys His Ser Val Leu Ser Leu 
            100                 105                 110         


Gly Thr Glu Thr Thr Ala Ser Met Ala Asp Gly Gln Lys Met Ser Lys 
        115                 120                 125             


Ile Gln Leu Trp Asp Glu Leu Lys Ile Glu Ser Leu Thr Arg Ile Val 
    130                 135                 140                 


Thr Leu Ile Tyr Cys Val Ser Leu Leu Asn Tyr Leu Ile Arg Leu Gln 
145                 150                 155                 160 


Thr Asn Ile Val Gly Arg Lys Arg Tyr Gln Asn Glu Ala Gly Pro Ala 
                165                 170                 175     


Gly Ala Thr Tyr Asp Met Ser Leu Glu Gln Cys Tyr Thr Trp Leu Leu 
            180                 185                 190         


Thr Arg Gly Trp Lys Ser Val Val Asp Asn Val Arg Arg Ser Val Gln 
        195                 200                 205             


Gln Val Phe Thr Gly Val Asn Pro Arg Gln Asn Leu Ser Leu Asp Glu 
    210                 215                 220                 


Phe Ala Thr Leu Leu Lys Arg Val Gln Thr Leu Val Asn Ser Pro Pro 
225                 230                 235                 240 


Tyr Ser Thr Thr Pro Asn Thr Phe Leu Thr Ser Leu Leu Pro Pro Arg 
                245                 250                 255     


Glu Leu Glu Gln Leu Arg Leu Glu Lys Glu Lys Gln Ser Leu Ser Pro 
            260                 265                 270         


Asn Tyr Thr Tyr Gly Ser Pro Leu Lys Asp Leu Val Phe Glu Ser Ala 
        275                 280                 285             


Gln His Ile Gln Ser Pro Gln Gly Met Ser Ser Phe Arg Ala Ile Ile 
    290                 295                 300                 


Asp Gln Ser Phe Lys Val Phe Leu Glu Lys Val Asn Glu Ser Gln Tyr 
305                 310                 315                 320 


Val Asn Pro Pro Ser Thr Gly Gly Lys Arg Ile Ala Val Gly Ala Leu 
                325                 330                 335     


Gln Pro Pro Ile Ile Ser Gly Gly Pro Lys Lys Val Lys Leu Ala Ser 
            340                 345                 350         


Leu Leu Ser Val Ala Thr Arg Gln Ser Ser Val Ile Ser His Ala Gln 
        355                 360                 365             


Pro Asn Pro Tyr Val Asp Ala Ile Asn Ser Val Ala Glu Tyr Asn Gly 
    370                 375                 380                 


Leu Cys Ala Val Ile Tyr Ser Ser Phe Glu Gln 
385                 390                 395 


<210>  55
<211>  153
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(153)
<223>  YlPex4p; GenBank Accession No. CAG79130

<400>  55

Met Ala Ser Gln Lys Arg Leu Ile Lys Glu Leu Ala Ala Tyr Lys Lys 
1               5                   10                  15      


Asp Pro Asn Pro Cys Leu Ala Ser Leu Thr Ala Asp Gly Asp Ser Leu 
            20                  25                  30          


Tyr Lys Trp Thr Ala Val Met Arg Gly Thr Glu Gly Thr Ala Tyr Glu 
        35                  40                  45              


Asn Gly Leu Trp Gln Val Glu Ile Asn Ile Pro Glu Asn Tyr Pro Leu 
    50                  55                  60                  


Gln Pro Pro Thr Met Phe Phe Arg Thr Lys Ile Cys His Pro Asn Ile 
65                  70                  75                  80  


His Phe Glu Thr Gly Glu Val Cys Ile Asp Val Leu Lys Thr Gln Trp 
                85                  90                  95      


Ser Pro Ala Trp Thr Ile Ser Ser Ala Cys Thr Ala Val Ser Ala Met 
            100                 105                 110         


Leu Ser Leu Pro Glu Pro Asp Ser Pro Leu Asn Ile Asp Ala Ala Asn 
        115                 120                 125             


Leu Val Arg Cys Gly Asp Glu Ser Ala Met Glu Gly Leu Val Arg Tyr 
    130                 135                 140                 


Tyr Val Asn Lys Tyr Ala Ser Gly Asn 
145                 150             


<210>  56
<211>  598
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(598)
<223>  YlPex5p; GenBank Accession No. CAG78803

<400>  56

Met Ser Phe Met Arg Gly Gly Ser Glu Cys Ser Thr Gly Arg Asn Pro 
1               5                   10                  15      


Leu Ser Gln Phe Thr Lys His Thr Ala Glu Asp Arg Ser Leu Gln His 
            20                  25                  30          


Asp Arg Val Ala Gly Pro Ser Gly Gly Arg Val Gly Gly Met Arg Ser 
        35                  40                  45              


Asn Thr Gly Glu Met Ser Gln Gln Asp Arg Glu Met Met Ala Arg Phe 
    50                  55                  60                  


Gly Ala Ala Gly Pro Glu Gln Ser Ser Phe Asn Tyr Glu Gln Met Arg 
65                  70                  75                  80  


His Glu Leu His Asn Met Gly Ala Gln Gly Gly Gln Ile Pro Gln Val 
                85                  90                  95      


Pro Ser Gln Gln Gly Ala Ala Asn Gly Gly Gln Trp Ala Arg Asp Phe 
            100                 105                 110         


Gly Gly Gln Gln Thr Ala Pro Gly Ala Ala Pro Gln Asp Ala Lys Asn 
        115                 120                 125             


Trp Asn Ala Glu Phe Gln Arg Gly Gly Ser Pro Ala Glu Ala Met Gln 
    130                 135                 140                 


Gln Gln Gly Pro Gly Pro Met Gln Gly Gly Met Gly Met Gly Gly Met 
145                 150                 155                 160 


Pro Met Tyr Gly Met Ala Arg Pro Met Tyr Ser Gly Met Ser Ala Asn 
                165                 170                 175     


Met Ala Pro Gln Phe Gln Pro Gln Gln Ala Asn Ala Arg Val Val Glu 
            180                 185                 190         


Leu Asp Glu Gln Asn Trp Glu Glu Gln Phe Lys Gln Met Asp Ser Ala 
        195                 200                 205             


Val Gly Lys Gly Lys Glu Val Glu Glu Gln Thr Ala Glu Thr Ala Thr 
    210                 215                 220                 


Ala Thr Glu Thr Val Thr Glu Thr Glu Thr Thr Thr Glu Asp Lys Pro 
225                 230                 235                 240 


Met Asp Ile Lys Asn Met Asp Phe Glu Asn Ile Trp Lys Asn Leu Gln 
                245                 250                 255     


Val Asn Val Leu Asp Asn Met Asp Glu Trp Leu Glu Glu Thr Asn Ser 
            260                 265                 270         


Pro Ala Trp Glu Arg Asp Phe His Glu Tyr Thr His Asn Arg Pro Glu 
        275                 280                 285             


Phe Ala Asp Tyr Gln Phe Glu Glu Asn Asn Gln Phe Met Glu His Pro 
    290                 295                 300                 


Asp Pro Phe Lys Ile Gly Val Glu Leu Met Glu Thr Gly Gly Arg Leu 
305                 310                 315                 320 


Ser Glu Ala Ala Leu Ala Phe Glu Ala Ala Val Gln Lys Asn Thr Glu 
                325                 330                 335     


His Ala Glu Ala Trp Gly Arg Leu Gly Ala Cys Gln Ala Gln Asn Glu 
            340                 345                 350         


Lys Glu Asp Pro Ala Ile Arg Ala Leu Glu Arg Cys Ile Lys Leu Glu 
        355                 360                 365             


Pro Gly Asn Leu Ser Ala Leu Met Asn Leu Ser Val Ser Tyr Thr Asn 
    370                 375                 380                 


Glu Gly Tyr Glu Asn Ala Ala Tyr Ala Thr Leu Glu Arg Trp Leu Ala 
385                 390                 395                 400 


Thr Lys Tyr Pro Glu Val Val Asp Gln Ala Arg Asn Gln Glu Pro Arg 
                405                 410                 415     


Leu Gly Asn Glu Asp Lys Phe Gln Leu His Ser Arg Val Thr Glu Leu 
            420                 425                 430         


Phe Ile Arg Ala Ala Gln Leu Ser Pro Asp Gly Ala Asn Ile Asp Ala 
        435                 440                 445             


Asp Val Gln Val Gly Leu Gly Val Leu Phe Tyr Gly Asn Glu Glu Tyr 
    450                 455                 460                 


Asp Lys Ala Ile Asp Cys Phe Asn Ala Ala Ile Ala Val Arg Pro Asp 
465                 470                 475                 480 


Asp Ala Leu Leu Trp Asn Arg Leu Gly Ala Thr Leu Ala Asn Ser His 
                485                 490                 495     


Arg Ser Glu Glu Ala Ile Asp Ala Tyr Tyr Lys Ala Leu Glu Leu Arg 
            500                 505                 510         


Pro Ser Phe Val Arg Ala Arg Tyr Asn Leu Gly Val Ser Cys Ile Asn 
        515                 520                 525             


Ile Gly Cys Tyr Lys Glu Ala Ala Gln Tyr Leu Leu Gly Ala Leu Ser 
    530                 535                 540                 


Met His Lys Val Glu Gly Val Gln Asp Asp Val Leu Ala Asn Gln Ser 
545                 550                 555                 560 


Thr Asn Leu Tyr Asp Thr Leu Lys Arg Val Phe Leu Gly Met Asp Arg 
                565                 570                 575     


Arg Asp Leu Val Ala Lys Val Gly Asn Gly Met Asp Val Asn Gln Phe 
            580                 585                 590         


Arg Asn Glu Phe Glu Phe 
        595             


<210>  57
<211>  1024
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(1024)
<223>  YlPex6p; GenBank Accession No. CAG82306

<400>  57

Met Pro Ser Ile Ser His Lys Pro Ile Thr Ala Lys Leu Val Ala Ala 
1               5                   10                  15      


Pro Asp Ala Thr Lys Leu Glu Leu Ser Ser Tyr Leu Tyr Gln Gln Leu 
            20                  25                  30          


Phe Ser Asp Lys Pro Ala Glu Pro Tyr Val Ala Phe Glu Ala Pro Gly 
        35                  40                  45              


Ile Lys Trp Ala Leu Tyr Pro Ala Ser Glu Asp Arg Ser Leu Pro Gln 
    50                  55                  60                  


Tyr Thr Cys Lys Ala Asp Ile Arg His Val Ala Gly Ser Leu Lys Lys 
65                  70                  75                  80  


Phe Met Pro Val Val Leu Lys Arg Val Asn Pro Val Thr Ile Glu His 
                85                  90                  95      


Ala Ile Val Thr Val Pro Ala Ser Gln Tyr Glu Thr Leu Asn Thr Pro 
            100                 105                 110         


Glu Gln Val Leu Lys Ala Leu Glu Pro Gln Leu Asp Lys Asp Arg Pro 
        115                 120                 125             


Val Ile Arg Gln Gly Asp Val Leu Leu Asn Gly Cys Arg Val Arg Leu 
    130                 135                 140                 


Cys Glu Pro Val Asn Gln Gly Lys Val Val Lys Gly Thr Thr Lys Leu 
145                 150                 155                 160 


Thr Val Ala Lys Glu Gln Glu Thr Ile Gln Pro Ala Asp Glu Ala Ala 
                165                 170                 175     


Asp Val Ala Phe Asp Ile Ala Glu Phe Leu Asp Phe Asp Thr Ser Val 
            180                 185                 190         


Ala Lys Thr Arg Glu Ser Thr Asn Leu Gln Val Ala Pro Leu Glu Gly 
        195                 200                 205             


Ala Ile Pro Thr Pro Leu Ser Asp Arg Phe Asp Asp Cys Glu Ser Arg 
    210                 215                 220                 


Gly Phe Val Lys Ser Glu Thr Met Ser Lys Leu Gly Val Phe Ser Gly 
225                 230                 235                 240 


Asp Ile Val Ser Ile Lys Thr Lys Asn Gly Ala Glu Arg Val Leu Arg 
                245                 250                 255     


Leu Phe Ala Tyr Pro Glu Pro Asn Thr Val Lys Tyr Asp Val Val Tyr 
            260                 265                 270         


Val Ser Pro Ile Leu Tyr His Asn Ile Gly Asp Lys Glu Ile Glu Val 
        275                 280                 285             


Thr Pro Asn Gly Glu Thr His Lys Ser Val Gly Glu Ala Leu Asp Ser 
    290                 295                 300                 


Val Leu Glu Ala Ala Glu Glu Val Lys Leu Ala Arg Val Leu Gly Pro 
305                 310                 315                 320 


Thr Thr Thr Asp Arg Thr Phe Gln Thr Ala Tyr His Ala Gly Leu Gln 
                325                 330                 335     


Ala Tyr Phe Lys Pro Val Lys Arg Ala Val Arg Val Gly Asp Leu Ile 
            340                 345                 350         


Pro Ile Pro Phe Asp Ser Ile Leu Ala Arg Thr Ile Gly Glu Asp Pro 
        355                 360                 365             


Glu Met Ser His Ile Pro Leu Glu Ala Leu Ala Val Lys Pro Asp Ser 
    370                 375                 380                 


Val Ala Trp Phe Gln Val Thr Ser Leu Asn Gly Ser Glu Asp Pro Ala 
385                 390                 395                 400 


Ser Lys Gln Tyr Leu Val Asp Ser Ser Gln Thr Lys Leu Ile Glu Gly 
                405                 410                 415     


Gly Thr Thr Ser Ser Ala Val Ile Pro Thr Ser Val Pro Trp Arg Glu 
            420                 425                 430         


Tyr Leu Gly Leu Asp Thr Leu Pro Lys Phe Gly Ser Glu Phe Ala Tyr 
        435                 440                 445             


Ala Asp Lys Ile Arg Asn Leu Val Gln Ile Ser Thr Ser Ala Leu Ser 
    450                 455                 460                 


His Ala Lys Leu Asn Thr Ser Val Leu Leu His Ser Ala Lys Arg Gly 
465                 470                 475                 480 


Val Gly Lys Ser Thr Val Leu Arg Ser Val Ala Ala Gln Cys Gly Ile 
                485                 490                 495     


Ser Val Phe Glu Ile Ser Cys Phe Gly Leu Ile Gly Asp Asn Glu Ala 
            500                 505                 510         


Gln Thr Leu Gly Thr Leu Arg Ala Lys Leu Asp Arg Ala Tyr Gly Cys 
        515                 520                 525             


Ser Pro Cys Val Val Val Leu Gln His Leu Glu Ser Ile Ala Lys Lys 
    530                 535                 540                 


Ser Asp Gln Asp Gly Lys Asp Glu Gly Ile Val Ser Lys Leu Val Asp 
545                 550                 555                 560 


Val Leu Ala Asp Tyr Ser Gly His Gly Val Leu Leu Ala Ala Thr Ser 
                565                 570                 575     


Asn Asp Pro Asp Lys Ile Ser Glu Ala Ile Arg Ser Arg Phe Gln Phe 
            580                 585                 590         


Glu Ile Glu Ile Gly Val Pro Ser Glu Pro Gln Arg Arg Gln Ile Phe 
        595                 600                 605             


Ser His Leu Thr Lys Ser Gly Pro Gly Gly Asp Ser Ile Arg Asn Ala 
    610                 615                 620                 


Pro Ile Ser Leu Arg Ser Asp Val Ser Val Glu Asn Leu Ala Leu Gln 
625                 630                 635                 640 


Ser Ala Gly Leu Thr Pro Pro Asp Leu Thr Ala Ile Val Gln Thr Thr 
                645                 650                 655     


Arg Leu Arg Ala Ile Asp Arg Leu Asn Lys Leu Thr Lys Asp Ser Asp 
            660                 665                 670         


Thr Thr Leu Asp Asp Leu Leu Thr Leu Ser His Gly Thr Leu Gln Leu 
        675                 680                 685             


Thr Pro Ser Asp Phe Asp Asp Ala Ile Ala Asp Ala Arg Gln Lys Tyr 
    690                 695                 700                 


Ser Asp Ser Ile Gly Ala Pro Arg Ile Pro Asn Val Gly Trp Asp Asp 
705                 710                 715                 720 


Val Gly Gly Met Glu Gly Val Lys Lys Asp Ile Leu Asp Thr Ile Glu 
                725                 730                 735     


Thr Pro Leu Lys Tyr Pro His Trp Phe Ser Asp Gly Val Lys Lys Arg 
            740                 745                 750         


Ser Gly Ile Leu Phe Tyr Gly Pro Pro Gly Thr Gly Lys Thr Leu Leu 
        755                 760                 765             


Ala Lys Ala Ile Ala Thr Thr Phe Ser Leu Asn Phe Phe Ser Val Lys 
    770                 775                 780                 


Gly Pro Glu Leu Leu Asn Met Tyr Ile Gly Glu Ser Glu Ala Asn Val 
785                 790                 795                 800 


Arg Arg Val Phe Gln Lys Ala Arg Asp Ala Lys Pro Cys Val Val Phe 
                805                 810                 815     


Phe Asp Glu Leu Asp Ser Val Ala Pro Gln Arg Gly Asn Gln Gly Asp 
            820                 825                 830         


Ser Gly Gly Val Met Asp Arg Ile Val Ser Gln Leu Leu Ala Glu Leu 
        835                 840                 845             


Asp Gly Met Ser Thr Ala Gly Gly Glu Gly Val Phe Val Val Gly Ala 
    850                 855                 860                 


Thr Asn Arg Pro Asp Leu Leu Asp Glu Ala Leu Leu Arg Pro Gly Arg 
865                 870                 875                 880 


Phe Asp Lys Met Leu Tyr Leu Gly Ile Ser Asp Thr His Glu Lys Gln 
                885                 890                 895     


Gln Thr Ile Met Glu Ala Leu Thr Arg Lys Phe Arg Leu Ala Ala Asp 
            900                 905                 910         


Val Ser Leu Glu Ala Ile Ser Lys Arg Cys Pro Phe Thr Phe Thr Gly 
        915                 920                 925             


Ala Asp Phe Tyr Ala Leu Cys Ser Asp Ala Met Leu Asn Ala Met Thr 
    930                 935                 940                 


Arg Thr Ala Asn Glu Val Asp Ala Lys Ile Lys Leu Leu Asn Lys Asn 
945                 950                 955                 960 


Arg Glu Glu Ala Gly Glu Glu Pro Val Ser Ile Arg Trp Trp Phe Asp 
                965                 970                 975     


His Glu Ala Thr Lys Ser Asp Ile Glu Val Glu Val Ala Gln Gln Asp 
            980                 985                 990         


Phe Glu Lys Ala Lys Asp Glu Leu  Ser Pro Ser Val Ser  Ala Glu Glu 
        995                 1000                 1005             


Leu Gln  His Tyr Leu Lys Leu  Arg Gln Gln Phe Glu  Gly Gly Lys 
    1010                 1015                 1020             


Lys 
    


<210>  58
<211>  356
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(356)
<223>  YlPex7p; GenBank Accession No. CAG78389

<400>  58

Met Leu Gly Phe Lys Thr Gln Gly Phe Asn Gly Tyr Ala Ala Asn Tyr 
1               5                   10                  15      


Ser Pro Phe Phe Asn Asp Lys Ile Ala Val Gly Thr Ala Ala Asn Tyr 
            20                  25                  30          


Gly Leu Val Gly Asn Gly Lys Leu Phe Ile Leu Gly Ile Ser Pro Glu 
        35                  40                  45              


Gly Arg Met Val Cys Glu Gly Gln Phe Asp Thr Gln Asp Gly Ile Phe 
    50                  55                  60                  


Asp Val Ala Trp Ser Glu Gln His Glu Asn His Val Ala Thr Ala Cys 
65                  70                  75                  80  


Gly Asp Gly Ser Val Lys Leu Phe Asp Ile Lys Ala Gly Ala Phe Pro 
                85                  90                  95      


Leu Val Ser Phe Lys Glu His Thr Arg Glu Val Phe Ser Val Asn Trp 
            100                 105                 110         


Asn Met Ala Asn Lys Ala Leu Phe Cys Thr Ser Ser Trp Asp Ser Thr 
        115                 120                 125             


Ile Lys Ile Trp Thr Pro Glu Arg Thr Asn Ser Ile Met Thr Leu Gly 
    130                 135                 140                 


Gln Pro Ala Pro Ala Gln Gly Thr Asn Ala Ser Ala His Ile Gly Arg 
145                 150                 155                 160 


Gln Thr Ala Pro Asn Gln Ala Ala Ala Gln Glu Cys Ile Tyr Ser Ala 
                165                 170                 175     


Lys Phe Ser Pro His Thr Asp Ser Ile Ile Ala Ser Ala His Ser Thr 
            180                 185                 190         


Gly Met Val Lys Val Trp Asp Thr Arg Ala Pro Gln Pro Leu Gln Gln 
        195                 200                 205             


Gln Phe Ser Thr Gln Gln Thr Glu Ser Gly Gly Pro Pro Glu Val Leu 
    210                 215                 220                 


Ser Leu Asp Trp Asn Lys Tyr Arg Pro Thr Val Ile Ala Thr Gly Gly 
225                 230                 235                 240 


Val Asp Arg Ser Val Gln Val Tyr Asp Ile Arg Met Thr Gln Pro Ala 
                245                 250                 255     


Ala Asn Gln Pro Val Gln Pro Leu Ser Leu Ile Leu Gly His Arg Leu 
            260                 265                 270         


Pro Val Arg Gly Val Ser Trp Ser Pro His His Ala Asp Leu Leu Leu 
        275                 280                 285             


Ser Cys Ser Tyr Asp Met Thr Ala Arg Val Trp Arg Asp Ala Ser Thr 
    290                 295                 300                 


Gly Gly Asn Tyr Leu Ala Arg Gln Arg Gly Gly Thr Glu Val Lys Cys 
305                 310                 315                 320 


Met Asp Arg His Thr Glu Phe Val Ile Gly Gly Asp Trp Ser Leu Trp 
                325                 330                 335     


Gly Asp Pro Gly Trp Ile Thr Thr Val Gly Trp Asp Gln Met Val Tyr 
            340                 345                 350         


Val Trp His Ala 
        355     


<210>  59
<211>  671
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(671)
<223>  YlPex8p; GenBank Accession No. CAG80447

<400>  59

Met Asn Lys Tyr Leu Val Pro Pro Pro Gln Ala Asn Arg Thr Val Thr 
1               5                   10                  15      


Asn Leu Asp Leu Leu Ile Asn Asn Leu Arg Gly Ser Ser Thr Pro Gly 
            20                  25                  30          


Ala Ala Glu Val Asp Thr Arg Asp Ile Leu Gln Arg Ile Val Phe Ile 
        35                  40                  45              


Leu Pro Thr Ile Lys Asn Pro Leu Asn Leu Asp Leu Val Ile Lys Glu 
    50                  55                  60                  


Ile Ile Asn Ser Pro Arg Leu Leu Pro Pro Leu Ile Asp Leu His Asp 
65                  70                  75                  80  


Tyr Gln Gln Leu Thr Asp Ala Phe Arg Ala Thr Ile Lys Arg Lys Ala 
                85                  90                  95      


Leu Val Thr Asp Pro Thr Ile Ser Phe Glu Ala Trp Leu Glu Thr Cys 
            100                 105                 110         


Phe Gln Val Ile Thr Arg Phe Ala Gly Pro Gly Trp Lys Lys Leu Pro 
        115                 120                 125             


Leu Leu Ala Gly Leu Ile Leu Ala Asp Tyr Asp Ile Ser Ala Asp Gly 
    130                 135                 140                 


Pro Thr Leu Glu Arg Lys Pro Gly Phe Pro Ser Lys Leu Lys His Leu 
145                 150                 155                 160 


Leu Lys Arg Glu Phe Val Thr Thr Phe Asp Gln Cys Leu Ser Ile Asp 
                165                 170                 175     


Thr Arg Asn Arg Ser Asp Ala Thr Lys Trp Val Pro Val Leu Ala Cys 
            180                 185                 190         


Ile Ser Ile Ala Gln Val Tyr Ser Leu Leu Gly Asp Val Ala Ile Asn 
        195                 200                 205             


Tyr Arg Arg Phe Leu Gln Val Gly Leu Asp Leu Ile Phe Ser Asn Tyr 
    210                 215                 220                 


Gly Leu Glu Met Gly Thr Ala Leu Ala Arg Leu His Ala Glu Ser Gly 
225                 230                 235                 240 


Gly Asp Ala Thr Thr Ala Gly Gly Leu Ile Gly Lys Lys Leu Lys Glu 
                245                 250                 255     


Pro Val Val Ala Leu Leu Asn Thr Phe Ala His Ile Ala Ser Ser Cys 
            260                 265                 270         


Ile Val His Val Asp Ile Asp Tyr Ile Asp Arg Ile Gln Asn Lys Ile 
        275                 280                 285             


Ile Leu Val Cys Glu Asn Gln Ala Glu Thr Trp Arg Ile Leu Thr Ile 
    290                 295                 300                 


Glu Ser Pro Thr Val Met His His Gln Glu Ser Val Gln Tyr Leu Lys 
305                 310                 315                 320 


Trp Glu Leu Phe Thr Leu Cys Ile Ile Met Gln Gly Ile Ala Asn Met 
                325                 330                 335     


Leu Leu Thr Gln Lys Met Asn Gln Phe Met Tyr Leu Gln Leu Ala Tyr 
            340                 345                 350         


Lys Gln Leu Gln Ala Leu His Ser Ile Tyr Phe Ile Val Asp Gln Met 
        355                 360                 365             


Gly Ser Gln Phe Ala Ala Tyr Asp Tyr Val Phe Phe Ser Ala Ile Asp 
    370                 375                 380                 


Val Leu Leu Ser Glu Tyr Ala Pro Tyr Ile Lys Asn Arg Gly Thr Ile 
385                 390                 395                 400 


Pro Pro Asn Lys Glu Phe Val Ala Glu Arg Leu Ala Ala Asn Leu Ala 
                405                 410                 415     


Gly Thr Ser Asn Val Gly Ser His Leu Pro Ile Asp Arg Ser Arg Val 
            420                 425                 430         


Leu Phe Ala Leu Asn Tyr Tyr Glu Gln Leu Val Thr Val Cys His Asp 
        435                 440                 445             


Ser Cys Val Glu Thr Ile Ile Tyr Pro Met Ala Arg Ser Phe Leu Tyr 
    450                 455                 460                 


Pro Thr Ser Asp Ile Gln Gln Leu Lys Pro Leu Val Glu Ala Ala His 
465                 470                 475                 480 


Ser Val Ile Leu Ala Gly Leu Ala Val Pro Thr Asn Ala Val Val Asn 
                485                 490                 495     


Ala Lys Leu Ile Pro Glu Tyr Met Gly Gly Val Leu Pro Leu Phe Pro 
            500                 505                 510         


Gly Val Phe Ser Trp Asn Gln Phe Val Leu Ala Ile Gln Ser Ile Val 
        515                 520                 525             


Asn Thr Val Ser Pro Pro Ser Glu Val Phe Lys Thr Asn Gln Lys Leu 
    530                 535                 540                 


Phe Arg Leu Val Leu Asp Ser Leu Met Lys Lys Cys Arg Asp Thr Pro 
545                 550                 555                 560 


Val Gly Ile Pro Val Pro His Ser Val Thr Val Ser Gln Glu Gln Glu 
                565                 570                 575     


Asp Ile Pro Pro Thr Gln Arg Ala Val Val Met Leu Ala Leu Ile Asn 
            580                 585                 590         


Ser Leu Pro Tyr Val Asp Ile Arg Ser Phe Glu Leu Trp Leu Gln Glu 
        595                 600                 605             


Thr Trp Asn Met Ile Glu Ala Thr Pro Met Leu Ala Glu Asn Ala Pro 
    610                 615                 620                 


Asn Lys Glu Leu Ala His Ala Glu His Glu Phe Leu Val Leu Glu Met 
625                 630                 635                 640 


Trp Lys Met Ile Ser Gly Asn Ile Asp Gln Arg Leu Asn Asp Val Ala 
                645                 650                 655     


Ile Arg Trp Trp Tyr Lys Lys Asn Ala Arg Val His Gly Thr Leu 
            660                 665                 670     


<210>  60
<211>  377
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(377)
<223>  YlPex10p; GenBank Accession No. CAG81606

<400>  60

Met Trp Gly Ser Ser His Ala Phe Ala Gly Glu Ser Asp Leu Thr Leu 
1               5                   10                  15      


Gln Leu His Thr Arg Ser Asn Met Ser Asp Asn Thr Thr Ile Lys Lys 
            20                  25                  30          


Pro Ile Arg Pro Lys Pro Ile Arg Thr Glu Arg Leu Pro Tyr Ala Gly 
        35                  40                  45              


Ala Ala Glu Ile Ile Arg Ala Asn Gln Lys Asp His Tyr Phe Glu Ser 
    50                  55                  60                  


Val Leu Glu Gln His Leu Val Thr Phe Leu Gln Lys Trp Lys Gly Val 
65                  70                  75                  80  


Arg Phe Ile His Gln Tyr Lys Glu Glu Leu Glu Thr Ala Ser Lys Phe 
                85                  90                  95      


Ala Tyr Leu Gly Leu Cys Thr Leu Val Gly Ser Lys Thr Leu Gly Glu 
            100                 105                 110         


Glu Tyr Thr Asn Leu Met Tyr Thr Ile Arg Asp Arg Thr Ala Leu Pro 
        115                 120                 125             


Gly Val Val Arg Arg Phe Gly Tyr Val Leu Ser Asn Thr Leu Phe Pro 
    130                 135                 140                 


Tyr Leu Phe Val Arg Tyr Met Gly Lys Leu Arg Ala Lys Leu Met Arg 
145                 150                 155                 160 


Glu Tyr Pro His Leu Val Glu Tyr Asp Glu Asp Glu Pro Val Pro Ser 
                165                 170                 175     


Pro Glu Thr Trp Lys Glu Arg Val Ile Lys Thr Phe Val Asn Lys Phe 
            180                 185                 190         


Asp Lys Phe Thr Ala Leu Glu Gly Phe Thr Ala Ile His Leu Ala Ile 
        195                 200                 205             


Phe Tyr Val Tyr Gly Ser Tyr Tyr Gln Leu Ser Lys Arg Ile Trp Gly 
    210                 215                 220                 


Met Arg Tyr Val Phe Gly His Arg Leu Asp Lys Asn Glu Pro Arg Ile 
225                 230                 235                 240 


Gly Tyr Glu Met Leu Gly Leu Leu Ile Phe Ala Arg Phe Ala Thr Ser 
                245                 250                 255     


Phe Val Gln Thr Gly Arg Glu Tyr Leu Gly Ala Leu Leu Glu Lys Ser 
            260                 265                 270         


Val Glu Lys Glu Ala Gly Glu Lys Glu Asp Glu Lys Glu Ala Val Val 
        275                 280                 285             


Pro Lys Lys Lys Ser Ser Ile Pro Phe Ile Glu Asp Thr Glu Gly Glu 
    290                 295                 300                 


Thr Glu Asp Lys Ile Asp Leu Glu Asp Pro Arg Gln Leu Lys Phe Ile 
305                 310                 315                 320 


Pro Glu Ala Ser Arg Ala Cys Thr Leu Cys Leu Ser Tyr Ile Ser Ala 
                325                 330                 335     


Pro Ala Cys Thr Pro Cys Gly His Phe Phe Cys Trp Asp Cys Ile Ser 
            340                 345                 350         


Glu Trp Val Arg Glu Lys Pro Glu Cys Pro Leu Cys Arg Gln Gly Val 
        355                 360                 365             


Arg Glu Gln Asn Leu Leu Pro Ile Arg 
    370                 375         


<210>  61
<211>  408
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(408)
<223>  YlPex12p; GenBank Accession No. CAG81532

<400>  61

Met Asp Tyr Phe Ser Ser Leu Asn Ala Ser Gln Leu Asp Pro Asp Val 
1               5                   10                  15      


Pro Thr Leu Phe Glu Leu Leu Ser Ala Lys Gln Leu Glu Gly Leu Ile 
            20                  25                  30          


Ala Pro Ser Val Arg Tyr Ile Leu Ala Phe Tyr Ala Gln Arg His Pro 
        35                  40                  45              


Arg Tyr Leu Leu Arg Ile Val Asn Arg Tyr Asp Glu Leu Tyr Ala Leu 
    50                  55                  60                  


Phe Met Gly Leu Val Glu Tyr Tyr Asn Leu Lys Thr Trp Asn Ala Ser 
65                  70                  75                  80  


Phe Thr Glu Lys Phe Tyr Gly Leu Lys Arg Thr Gln Ile Leu Thr Asn 
                85                  90                  95      


Pro Ala Leu Arg Thr Arg Gln Ala Val Pro Asp Leu Val Glu Ala Glu 
            100                 105                 110         


Lys Arg Leu Ser Lys Lys Lys Ile Trp Gly Ser Leu Phe Phe Leu Ile 
        115                 120                 125             


Val Val Pro Tyr Val Lys Glu Lys Leu Asp Ala Arg Tyr Glu Arg Leu 
    130                 135                 140                 


Lys Gly Arg Tyr Leu Ala Arg Asp Ile Asn Glu Glu Arg Ile Glu Ile 
145                 150                 155                 160 


Lys Arg Thr Gly Thr Ala Gln Gln Ile Ala Val Phe Glu Phe Asp Tyr 
                165                 170                 175     


Trp Leu Leu Lys Leu Tyr Pro Ile Val Thr Met Gly Cys Thr Thr Ala 
            180                 185                 190         


Thr Leu Ala Phe His Met Leu Phe Leu Phe Ser Val Thr Arg Ala Tyr 
        195                 200                 205             


Ser Ile Asp Asp Phe Leu Leu Asn Ile Gln Phe Ser Arg Met Thr Arg 
    210                 215                 220                 


Tyr Asp Tyr Gln Met Glu Thr Gln Arg Asp Ser Arg Asn Ala Ala Asn 
225                 230                 235                 240 


Val Ala His Thr Met Lys Ser Ile Ser Glu Tyr Pro Val Ala Glu Arg 
                245                 250                 255     


Val Met Leu Leu Leu Thr Thr Lys Ala Gly Ala Asn Ala Met Arg Ser 
            260                 265                 270         


Ala Ala Leu Ser Gly Leu Ser Tyr Val Leu Pro Thr Ser Ile Phe Ala 
        275                 280                 285             


Leu Lys Phe Leu Glu Trp Trp Tyr Ala Ser Asp Phe Ala Arg Gln Leu 
    290                 295                 300                 


Asn Gln Lys Arg Arg Gly Asp Leu Glu Asp Asn Leu Pro Val Pro Asp 
305                 310                 315                 320 


Lys Val Lys Gly Ala Asp Lys Leu Ala Glu Ser Val Ala Lys Trp Lys 
                325                 330                 335     


Glu Asp Thr Ser Lys Cys Pro Leu Cys Ser Lys Glu Leu Val Asn Pro 
            340                 345                 350         


Thr Val Ile Glu Ser Gly Tyr Val Phe Cys Tyr Thr Cys Ile Tyr Arg 
        355                 360                 365             


His Leu Glu Asp Gly Asp Glu Glu Thr Gly Gly Arg Cys Pro Val Thr 
    370                 375                 380                 


Gly Gln Lys Leu Leu Gly Cys Arg Trp Gln Asp Asp Val Trp Gln Val 
385                 390                 395                 400 


Thr Gly Leu Arg Arg Leu Met Val 
                405             


<210>  62
<211>  412
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(412)
<223>  YlPex13p; GenBank Accession No. CAG81789

<400>  62

Met Ser Val Pro Arg Pro Lys Pro Trp Glu Gly Ala Ser Gly Ser Ser 
1               5                   10                  15      


Ala Ala Thr Ala Thr Pro Ala Ala Thr Ala Thr Pro Ala Ser Thr Asp 
            20                  25                  30          


Ala Val Ser Ser Ser Ala Gly Ser Ala Thr Gly Ala Pro Glu Leu Pro 
        35                  40                  45              


Ser Arg Pro Ser Ala Met Gly Ser Thr Ser Asn Ala Leu Ser Ser Pro 
    50                  55                  60                  


Met Gly Ser Ser Met Asn Ser Gly Tyr Gly Gly Met Asn Ser Gly Tyr 
65                  70                  75                  80  


Gly Gly Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Ser Tyr Gly 
                85                  90                  95      


Met Gly Ser Ser Tyr Gly Ser Gly Tyr Gly Ser Gly Leu Gly Gly Tyr 
            100                 105                 110         


Gly Ser Tyr Gly Gly Met Gly Gly Met Gly Gly Met Tyr Gly Ser Arg 
        115                 120                 125             


Tyr Gly Gly Tyr Gly Ser Tyr Gly Gly Met Gly Gly Tyr Gly Gly Tyr 
    130                 135                 140                 


Gly Gly Met Gly Gly Gly Pro Met Gly Gln Asn Gly Leu Ala Gly Gly 
145                 150                 155                 160 


Thr Gln Ala Thr Phe Gln Leu Ile Glu Ser Ile Val Gly Ala Val Gly 
                165                 170                 175     


Gly Phe Ala Gln Met Leu Glu Ser Thr Tyr Met Ala Thr Gln Ser Ser 
            180                 185                 190         


Phe Phe Ala Met Val Ser Val Ala Glu Gln Phe Gly Asn Leu Lys Asn 
        195                 200                 205             


Thr Leu Gly Ser Leu Leu Gly Ile Tyr Ala Ile Met Arg Trp Ala Arg 
    210                 215                 220                 


Arg Leu Val Ala Lys Leu Ser Gly Gln Pro Val Thr Gly Ala Asn Gly 
225                 230                 235                 240 


Ile Thr Pro Ala Gly Phe Ala Lys Phe Glu Ala Thr Gly Gly Ala Ala 
                245                 250                 255     


Gly Pro Gly Arg Gly Pro Arg Pro Ser Tyr Lys Pro Leu Leu Phe Phe 
            260                 265                 270         


Leu Thr Ala Val Phe Gly Leu Pro Tyr Leu Leu Gly Arg Leu Ile Lys 
        275                 280                 285             


Ala Leu Ala Ala Lys Gln Glu Gly Met Tyr Asp Glu His Gly Asn Leu 
    290                 295                 300                 


Leu Pro Gly Ala Gln Met Gly Met Gly Gly Pro Gly Met Glu Gly Gly 
305                 310                 315                 320 


Ala Glu Ile Asp Pro Ser Lys Leu Glu Phe Cys Arg Ala Asn Phe Asp 
                325                 330                 335     


Phe Val Pro Glu Asn Pro Gln Leu Glu Leu Glu Leu Arg Lys Gly Asp 
            340                 345                 350         


Leu Val Ala Val Leu Ala Lys Thr Asp Pro Met Gly Asn Pro Ser Gln 
        355                 360                 365             


Trp Trp Arg Val Arg Thr Arg Asp Gly Arg Ser Gly Tyr Val Pro Ala 
    370                 375                 380                 


Asn Tyr Leu Glu Val Ile Pro Arg Pro Ala Val Glu Ala Pro Lys Lys 
385                 390                 395                 400 


Val Glu Glu Ile Gly Ala Ser Ala Val Pro Val Asn 
                405                 410         


<210>  63
<211>  380
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(380)
<223>  YlPex14p; GenBank Accession No. CAG79323

<400>  63

Met Ile Pro Ser Cys Leu Ser Thr Gln His Met Ala Pro Arg Glu Asp 
1               5                   10                  15      


Leu Val Gln Ser Ala Val Ala Phe Leu Asn Asp Pro Gln Ala Ala Thr 
            20                  25                  30          


Ala Pro Leu Ala Lys Arg Ile Glu Phe Leu Glu Ser Lys Asp Met Thr 
        35                  40                  45              


Pro Glu Glu Ile Glu Glu Ala Leu Lys Arg Ala Gly Ser Gly Ser Ala 
    50                  55                  60                  


Gln Ser His Pro Gly Ser Val Val Ser His Gly Gly Ala Ala Pro Thr 
65                  70                  75                  80  


Val Pro Ala Ser Tyr Ala Phe Gln Ser Ala Pro Pro Leu Pro Glu Arg 
                85                  90                  95      


Asp Trp Lys Asp Val Phe Ile Met Ala Thr Val Thr Val Gly Val Gly 
            100                 105                 110         


Phe Gly Leu Tyr Thr Val Ala Lys Arg Tyr Leu Met Pro Leu Ile Leu 
        115                 120                 125             


Pro Pro Thr Pro Pro Ser Leu Glu Ala Asp Lys Glu Ala Leu Glu Ala 
    130                 135                 140                 


Glu Phe Ala Arg Val Gln Gly Leu Leu Asp Gln Val Gln Gln Asp Thr 
145                 150                 155                 160 


Glu Glu Val Lys Asn Ser Gln Val Glu Val Ala Lys Arg Val Thr Asp 
                165                 170                 175     


Ala Leu Lys Gly Val Glu Glu Thr Ile Asp Gln Leu Lys Ser Gln Thr 
            180                 185                 190         


Lys Lys Arg Asp Asp Glu Met Lys Leu Val Thr Ala Glu Val Glu Arg 
        195                 200                 205             


Ile Arg Asp Arg Leu Pro Lys Asn Ile Asp Lys Leu Lys Asp Ser Gln 
    210                 215                 220                 


Glu Gln Gly Leu Ala Asp Ile Gln Ser Glu Leu Lys Ser Leu Lys Gln 
225                 230                 235                 240 


Leu Leu Ser Thr Arg Thr Ala Ala Ser Ser Gly Pro Lys Leu Pro Pro 
                245                 250                 255     


Ile Pro Pro Pro Ser Ser Tyr Leu Thr Arg Lys Ala Ser Pro Ala Val 
            260                 265                 270         


Pro Ala Ala Ala Pro Ala Pro Val Thr Pro Gly Ser Pro Val His Asn 
        275                 280                 285             


Val Ser Ser Ser Ser Thr Val Pro Ala Asp Arg Asp Asp Phe Ile Pro 
    290                 295                 300                 


Thr Pro Ala Gly Ala Val Pro Met Ile Pro Gln Pro Ala Ser Met Ser 
305                 310                 315                 320 


Ser Ser Ser Thr Ser Thr Val Pro Asn Ser Ala Ile Ser Ser Ala Pro 
                325                 330                 335     


Ser Pro Ile Gln Glu Pro Glu Pro Phe Val Pro Glu Pro Gly Asn Ser 
            340                 345                 350         


Ala Val Lys Lys Pro Ala Pro Lys Ala Ser Ile Pro Ala Trp Gln Leu 
        355                 360                 365             


Ala Ala Leu Glu Lys Glu Lys Glu Lys Glu Lys Glu 
    370                 375                 380 


<210>  64
<211>  391
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(391)
<223>  YlPex16p; GenBank Accession No. CAG79622

<400>  64

Met Thr Asp Lys Leu Val Lys Val Met Gln Lys Lys Lys Ser Ala Pro 
1               5                   10                  15      


Gln Thr Trp Leu Asp Ser Tyr Asp Lys Phe Leu Val Arg Asn Ala Ala 
            20                  25                  30          


Ser Ile Gly Ser Ile Glu Ser Thr Leu Arg Thr Val Ser Tyr Val Leu 
        35                  40                  45              


Pro Gly Arg Phe Asn Asp Val Glu Ile Ala Thr Glu Thr Leu Tyr Ala 
    50                  55                  60                  


Val Leu Asn Val Leu Gly Leu Tyr His Asp Thr Ile Ile Ala Arg Ala 
65                  70                  75                  80  


Val Ala Ala Ser Pro Asn Ala Ala Ala Val Tyr Arg Pro Ser Pro His 
                85                  90                  95      


Asn Arg Tyr Thr Asp Trp Phe Ile Lys Asn Arg Lys Gly Tyr Lys Tyr 
            100                 105                 110         


Ala Ser Arg Ala Val Thr Phe Val Lys Phe Gly Glu Leu Val Ala Glu 
        115                 120                 125             


Met Val Ala Lys Lys Asn Gly Gly Glu Met Ala Arg Trp Lys Cys Ile 
    130                 135                 140                 


Ile Gly Ile Glu Gly Ile Lys Ala Gly Leu Arg Ile Tyr Met Leu Gly 
145                 150                 155                 160 


Ser Thr Leu Tyr Gln Pro Leu Cys Thr Thr Pro Tyr Pro Asp Arg Glu 
                165                 170                 175     


Val Thr Gly Glu Leu Leu Glu Thr Ile Cys Arg Asp Glu Gly Glu Leu 
            180                 185                 190         


Asp Ile Glu Lys Gly Leu Met Asp Pro Gln Trp Lys Met Pro Arg Thr 
        195                 200                 205             


Gly Arg Thr Ile Pro Glu Ile Ala Pro Thr Asn Val Glu Gly Tyr Leu 
    210                 215                 220                 


Leu Thr Lys Val Leu Arg Ser Glu Asp Val Asp Arg Pro Tyr Asn Leu 
225                 230                 235                 240 


Leu Ser Arg Leu Asp Asn Trp Gly Val Val Ala Glu Leu Leu Ser Ile 
                245                 250                 255     


Leu Arg Pro Leu Ile Tyr Ala Cys Leu Leu Phe Arg Gln His Val Asn 
            260                 265                 270         


Lys Thr Val Pro Ala Ser Thr Lys Ser Lys Phe Pro Phe Leu Asn Ser 
        275                 280                 285             


Pro Trp Ala Pro Trp Ile Ile Gly Leu Val Ile Glu Ala Leu Ser Arg 
    290                 295                 300                 


Lys Met Met Gly Ser Trp Leu Leu Arg Gln Arg Gln Ser Gly Lys Thr 
305                 310                 315                 320 


Pro Thr Ala Leu Asp Gln Met Glu Val Lys Gly Arg Thr Asn Leu Leu 
                325                 330                 335     


Gly Trp Trp Leu Phe Arg Gly Glu Phe Tyr Gln Ala Tyr Thr Arg Pro 
            340                 345                 350         


Leu Leu Tyr Ser Ile Val Ala Arg Leu Glu Lys Ile Pro Gly Leu Gly 
        355                 360                 365             


Leu Phe Gly Ala Leu Ile Ser Asp Tyr Leu Tyr Leu Phe Asp Arg Tyr 
    370                 375                 380                 


Tyr Phe Thr Ala Ser Thr Leu 
385                 390     


<210>  65
<211>  225
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(225)
<223>  YlPex17p; GenBank Accession No. CAG84025

<400>  65

Met Ser Ala Phe Pro Glu Pro Ser Ser Phe Glu Ile Glu Phe Ala Lys 
1               5                   10                  15      


Gln Met Asn Arg Pro Arg Thr Val Gln Phe Lys Gln Leu Val Ala Val 
            20                  25                  30          


Leu Tyr Ile Phe Gly Gly Thr Ser Ala Leu Ile Tyr Ile Ile Ser Lys 
        35                  40                  45              


Thr Ile Leu Asn Pro Leu Phe Glu Glu Leu Thr Phe Ala Arg Ser Glu 
    50                  55                  60                  


Tyr Ala Ile His Ala Arg Arg Leu Met Glu Gln Leu Asn Ala Lys Leu 
65                  70                  75                  80  


Ser Ser Met Ala Ser Tyr Ile Pro Pro Val Arg Ala Leu Gln Gly Gln 
                85                  90                  95      


Arg Phe Val Asp Ala Gln Thr Gln Thr Glu Asp Glu Glu Gly Glu Asp 
            100                 105                 110         


Ile Pro Asn Pro Ser Leu Gly Lys Ser Ser His Val Ser Phe Gly Glu 
        115                 120                 125             


Ser Pro Met Gln Leu Lys Leu Ala Glu Lys Glu Lys Gln Gln Lys Leu 
    130                 135                 140                 


Ile Asp Asp Ser Val Asp Asn Leu Glu Arg Leu Ala Asp Ser Leu Lys 
145                 150                 155                 160 


His Ala Gly Glu Val Ser Asp Leu Ser Ala Leu Ser Gly Phe Lys Tyr 
                165                 170                 175     


Gln Val Glu Glu Leu Thr Asn Tyr Ser Asp Gln Leu Ala Met Ser Gly 
            180                 185                 190         


Tyr Ser Met Met Lys Ser Gly Leu Pro Gly His Glu Thr Ala Met Ser 
        195                 200                 205             


Glu Thr Lys Lys Glu Ile Arg Ser Leu Lys Gly Ser Val Leu Ser Val 
    210                 215                 220                 


Arg 
225 


<210>  66
<211>  324
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(324)
<223>  YlPex19p; GenBank Accession No. AAK84827

<400>  66

Met Ser His Glu Glu Asp Leu Asp Asp Leu Asp Asp Phe Leu Asp Glu 
1               5                   10                  15      


Phe Asp Glu Gln Val Leu Ser Lys Pro Pro Gly Ala Gln Lys Asp Ala 
            20                  25                  30          


Thr Pro Thr Thr Ser Thr Ala Pro Thr Thr Ala Glu Ala Lys Pro Asp 
        35                  40                  45              


Ala Thr Lys Lys Ser Thr Glu Thr Ser Gly Thr Asp Ser Lys Thr Glu 
    50                  55                  60                  


Gly Ala Asp Thr Ala Asp Lys Asn Ala Ala Thr Asp Ser Ala Glu Ala 
65                  70                  75                  80  


Gly Ala Glu Lys Val Ser Leu Pro Asn Leu Glu Asp Gln Leu Ala Gly 
                85                  90                  95      


Leu Lys Met Asp Asp Phe Leu Lys Asp Ile Glu Ala Asp Pro Glu Ser 
            100                 105                 110         


Lys Ala Gln Phe Glu Ser Leu Leu Lys Glu Ile Asn Asn Val Thr Ser 
        115                 120                 125             


Ala Thr Ala Ser Glu Lys Ala Gln Gln Pro Lys Ser Phe Lys Glu Thr 
    130                 135                 140                 


Ile Ser Ala Thr Ala Asp Arg Leu Asn Gln Ser Asn Gln Glu Met Gly 
145                 150                 155                 160 


Asp Met Pro Leu Gly Asp Asp Met Leu Ala Gly Leu Met Glu Gln Leu 
                165                 170                 175     


Ser Gly Ala Gly Gly Phe Gly Glu Gly Gly Glu Gly Asp Phe Gly Asp 
            180                 185                 190         


Met Leu Gly Gly Ile Met Arg Gln Leu Ala Ser Lys Glu Val Leu Tyr 
        195                 200                 205             


Gln Pro Leu Lys Glu Met His Asp Asn Tyr Pro Lys Trp Trp Asp Glu 
    210                 215                 220                 


His Gly Ser Lys Val Thr Glu Glu Lys Glu Arg Asp Arg Leu Lys Leu 
225                 230                 235                 240 


Gln Gln Asp Ile Val Gly Lys Ile Cys Ala Lys Phe Glu Asp Pro Ser 
                245                 250                 255     


Tyr Ser Asp Asp Ser Glu Ala Asp Arg Ala Val Ile Thr Gln Leu Met 
            260                 265                 270         


Asp Glu Met Gln Glu Thr Gly Ala Pro Pro Asp Glu Ile Met Ser Asn 
        275                 280                 285             


Val Ala Asp Gly Ser Ile Pro Gly Gly Leu Asp Gly Leu Gly Leu Gly 
    290                 295                 300                 


Gly Leu Gly Gly Gly Lys Met Pro Glu Met Pro Glu Asn Met Pro Glu 
305                 310                 315                 320 


Cys Asn Gln Gln 
                


<210>  67
<211>  417
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(417)
<223>  YlPex20p; GenBank Accession No. CAG79226

<400>  67

Met Ala Ser Cys Gly Pro Ser Asn Ala Leu Gln Asn Leu Ser Lys His 
1               5                   10                  15      


Ala Ser Ala Asp Arg Ser Leu Gln His Asp Arg Met Ala Pro Gly Gly 
            20                  25                  30          


Ala Pro Gly Ala Gln Arg Gln Gln Phe Arg Ser Gln Thr Gln Gly Gly 
        35                  40                  45              


Gln Leu Asn Asn Glu Phe Gln Gln Phe Ala Gln Ala Gly Pro Ala His 
    50                  55                  60                  


Asn Ser Phe Glu Gln Ser Gln Met Gly Pro His Phe Gly Gln Gln His 
65                  70                  75                  80  


Phe Gly Gln Pro His Gln Pro Gln Met Gly Gln His Ala Pro Met Ala 
                85                  90                  95      


His Gly Gln Gln Ser Asp Trp Ala Gln Ser Phe Ser Gln Leu Asn Leu 
            100                 105                 110         


Gly Pro Gln Thr Gly Pro Gln His Thr Gln Gln Ser Asn Trp Gly Gln 
        115                 120                 125             


Asp Phe Met Arg Gln Ser Pro Gln Ser His Gln Val Gln Pro Gln Met 
    130                 135                 140                 


Ala Asn Gly Val Met Gly Ser Met Ser Gly Met Ser Ser Phe Gly Pro 
145                 150                 155                 160 


Met Tyr Ser Asn Ser Gln Leu Met Asn Ser Thr Tyr Gly Leu Gln Thr 
                165                 170                 175     


Glu His Gln Gln Thr His Lys Thr Glu Thr Lys Ser Ser Gln Asp Ala 
            180                 185                 190         


Ala Phe Glu Ala Ala Phe Gly Ala Val Glu Glu Ser Ile Thr Lys Thr 
        195                 200                 205             


Ser Asp Lys Gly Lys Glu Val Glu Lys Asp Pro Met Glu Gln Thr Tyr 
    210                 215                 220                 


Arg Tyr Asp Gln Ala Asp Ala Leu Asn Arg Gln Ala Glu His Ile Ser 
225                 230                 235                 240 


Asp Asn Ile Ser Arg Glu Glu Val Asp Ile Lys Thr Asp Glu Asn Gly 
                245                 250                 255     


Glu Phe Ala Ser Ile Ala Arg Gln Ile Ala Ser Ser Leu Glu Glu Ala 
            260                 265                 270         


Asp Lys Ser Lys Phe Glu Lys Ser Thr Phe Met Asn Leu Met Arg Arg 
        275                 280                 285             


Ile Gly Asn His Glu Val Thr Leu Asp Gly Asp Lys Leu Val Asn Lys 
    290                 295                 300                 


Glu Gly Glu Asp Ile Arg Glu Glu Val Arg Asp Glu Leu Leu Arg Glu 
305                 310                 315                 320 


Gly Ala Ser Gln Glu Asn Gly Phe Gln Ser Glu Ala Gln Gln Thr Ala 
                325                 330                 335     


Pro Leu Pro Val His His Glu Ala Pro Pro Pro Glu Gln Ile His Pro 
            340                 345                 350         


His Thr Glu Thr Gly Asp Lys Gln Leu Glu Asp Pro Met Val Tyr Ile 
        355                 360                 365             


Glu Gln Glu Ala Ala Arg Arg Ala Ala Glu Ser Gly Arg Thr Val Glu 
    370                 375                 380                 


Glu Glu Lys Leu Asn Phe Tyr Ser Pro Phe Glu Tyr Ala Gln Lys Leu 
385                 390                 395                 400 


Gly Pro Gln Gly Val Ala Lys Gln Ser Asn Trp Glu Glu Asp Tyr Asp 
                405                 410                 415     


Phe 
    


<210>  68
<211>  195
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(195)
<223>  YlPex22p; GenBank Accession No. CAG77876

<400>  68

Val Pro Arg Cys Thr Ser His Pro Cys Asn Leu Thr Leu His Leu Pro 
1               5                   10                  15      


Val Thr Thr Met Ala Pro Arg Lys Thr Arg Leu Pro Ala Val Ile Gly 
            20                  25                  30          


Ala Ala Ala Ala Ala Ala Ala Val Ala Tyr Leu Val Tyr Ser Phe Val 
        35                  40                  45              


Ala Lys Ser Asn Ser Asp Gln Asp Thr Phe Asp Ser Ser Val Gln Ser 
    50                  55                  60                  


Ser Ser Lys Ser Ser Thr Lys Ser Pro Lys Ser Thr Ala Thr Asn Ser 
65                  70                  75                  80  


Lys Ile Thr Val Val Val Ser Gln Glu Leu Val Gln Ser Gln Leu Val 
                85                  90                  95      


Asp Phe Lys His Leu Met Ser Val His Pro Asn Leu Val Val Ile Val 
            100                 105                 110         


Pro Pro Met Val Ala Asn Lys Phe His Arg Ala Leu Lys Ser Ser Val 
        115                 120                 125             


Gly His Asp His Gly Val Lys Val Ile Arg Cys Asp Thr Asp Val Gly 
    130                 135                 140                 


Val Ile His Val Ile Lys His Ile Arg Pro Asp Leu Ala Leu Ile Ala 
145                 150                 155                 160 


Asp Gly Val Gly Asp Asn Ile Gln Gly Glu Ile Lys Arg Phe Val Gly 
                165                 170                 175     


Ser Ser Glu Ala Leu Ser Gly Asp Val Asn Leu Ala Ala Glu Arg Leu 
            180                 185                 190         


Thr Gly Leu 
        195 


<210>  69
<211>  386
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<222>  (1)..(386)
<223>  YlPex26p; GenBank Accession No. NC_006072, antisense translation 
       of nucleotides 117230-118387

<400>  69

Met Pro Pro Ala Met Pro Gln Met Thr Thr Ser Thr Leu Leu Thr Asp 
1               5                   10                  15      


Ser Val Thr Ser Ala Val Asn Gln Ala Ala Thr Pro Lys Val Asp Gln 
            20                  25                  30          


Met Tyr Gln Thr Phe Gly Glu Ser Ala Arg Glu Phe Val Asn Lys Asn 
        35                  40                  45              


Phe Tyr Asn Ser Tyr Glu Leu Ile Arg Pro Phe Phe Asp Glu Ile Thr 
    50                  55                  60                  


Ala Lys Gly Ala Gln Gln Asn Gly Ser Thr Val Leu Asp Ala Glu Asn 
65                  70                  75                  80  


Pro His Asn Ile Pro Leu Ser Leu Trp Ile Lys Val Trp Ser Leu Tyr 
                85                  90                  95      


Leu Ala Ile Leu Asp Ala Ser Cys Lys Gln Ala Gly Glu Ala Leu Leu 
            100                 105                 110         


Asn Ser Thr Gly Asp Leu Ser Gly Ser Asp Ser Gly Glu Trp Asn Gln 
        115                 120                 125             


Thr Arg Lys Leu Leu Ala Arg Lys Leu Thr Ser Gly Ser Val Trp Asp 
    130                 135                 140                 


Glu Leu Val Thr Ala Ser Gly Gly Thr Gly Asn Ile His Pro Thr Ile 
145                 150                 155                 160 


Leu Ala Leu Leu Ala Ser Leu Ser Ile Arg His Asp Thr Asp Ala Lys 
                165                 170                 175     


Leu Met Ala Asp Asn Leu Glu Lys Phe Ile Val Thr Tyr Asn Asp Asn 
            180                 185                 190         


Gly Ser Asp Asp Val Lys Thr Lys Thr Ala Phe Tyr Lys Val Leu Asp 
        195                 200                 205             


Leu Tyr Leu Leu Arg Val Leu Pro Asp Leu Gly Gln Trp Asp Val Ala 
    210                 215                 220                 


His Ser Phe Val Asn Asn Thr Asn Leu Phe Ser His Glu Gln Lys Lys 
225                 230                 235                 240 


Glu Met Thr His Lys Leu Asp Gln Ser Gln Lys His Ala Glu Gln Glu 
                245                 250                 255     


His Lys Arg Leu Leu Glu Glu Ala Gln Glu Lys Glu Lys Ser Asp Ala 
            260                 265                 270         


Lys Glu Lys Glu Arg Glu Glu Arg Val Ser Arg Asp Thr Gln Ser Arg 
        275                 280                 285             


Glu Ile Lys Ser Pro Ile Val Asp Ser Ser Thr Ser Ser Arg Asp Val 
    290                 295                 300                 


Thr Arg Asp Thr Thr Arg Glu Leu Ser Lys Ser Ser Arg Gln Pro Arg 
305                 310                 315                 320 


Thr Leu Ser Gln Ile Ile Ser Thr Ser Leu Lys Ser Gln Phe Asp Gly 
                325                 330                 335     


Asn Ala Ile Phe Arg Thr Leu Ala Leu Ile Val Ile Val Ser Leu Ser 
            340                 345                 350         


Ala Ala Asn Pro Leu Ile Arg Lys Arg Val Val Asp Thr Leu Lys Met 
        355                 360                 365             


Leu Trp Ile Lys Ile Leu Gln Thr Leu Ser Met Gly Phe Lys Val Ser 
    370                 375                 380                 


Tyr Leu 
385     


<210>  70
<211>  10
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is a, c, g, or t

<300>
<302>  CODON-OPTIMIZED GENES FOR THE PRODUCTION OF POLYUNSATURATED FATTY
        ACIDS IN OLEAGINOUS YEAST
<310>  US 7,838,651
<311>  2008-01-23
<312>  2010-11-23
<313>  (1)..(10)

<400>  70
mammatgnhs                                                              10


<210>  71
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant His box


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  71

Gln Xaa Xaa His His 
1               5   


<210>  72
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His box


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  72

His Xaa Xaa His His 
1               5   


<210>  73
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = L or F

<220>
<221>  misc_feature
<222>  (5)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(13)

<400>  73

Tyr Asn Xaa Xaa Xaa Xaa Xaa Xaa Ser Xaa Xaa Ser Phe 
1               5                   10              


<210>  74
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X = E or D

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  X = T or S

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(15)

<400>  74

Phe Tyr Xaa Ser Lys Xaa Xaa Xaa Tyr Xaa Asp Xaa Xaa Xaa Leu 
1               5                   10                  15  


<210>  75
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  X = Q or H

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(9)

<400>  75

Leu Xaa Xaa Phe His His Xaa Gly Ala 
1               5                   


<210>  76
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(12)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  X = K or R or N

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(14)

<400>  76

Met Tyr Xaa Tyr Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe 
1               5                   10                  


<210>  77
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = I or L or M

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(8)

<400>  77

Lys Xaa Leu Xaa Thr Xaa Xaa Gln 
1               5               


<210>  78
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(7)

<400>  78

Trp Xaa Phe Asn Tyr Xaa Tyr 
1               5           


<210>  79
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(9)

<400>  79

Tyr Xaa Gly Xaa Val Xaa Xaa Leu Phe 
1               5                   


<210>  80
<211>  1449
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1449)
<223>  delta-9 desaturase; GenBank Accession No. XM_501496

<400>  80
atg gtg aaa aac gtg gac caa gtg gat ctc tcg cag gtc gac acc att         48
Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile           
1               5                   10                  15                

gcc tcc ggc cga gat gtc aac tac aag gtc aag tac acc tcc ggc gtt         96
Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val           
            20                  25                  30                    

aag atg agc cag ggc gcc tac gac gac aag ggc cgc cac att tcc gag        144
Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu           
        35                  40                  45                        

cag ccc ttc acc tgg gcc aac tgg cac cag cac atc aac tgg ctc aac        192
Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn           
    50                  55                  60                            

ttc att ctg gtg att gcg ctg cct ctg tcg tcc ttt gct gcc gct ccc        240
Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro           
65                  70                  75                  80            

ttc gtc tcc ttc aac tgg aag acc gcc gcg ttt gct gtc ggc tat tac        288
Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr           
                85                  90                  95                

atg tgc acc ggt ctc ggt atc acc gcc ggc tac cac cga atg tgg gcc        336
Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala           
            100                 105                 110                   

cat cga gcc tac aag gcc gct ctg ccc gtt cga atc atc ctt gct ctg        384
His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu           
        115                 120                 125                       

ttt gga gga gga gct gtc gag ggc tcc atc cga tgg tgg gcc tcg tct        432
Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser           
    130                 135                 140                           

cac cga gtc cac cac cga tgg acc gac tcc aac aag gac cct tac gac        480
His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp           
145                 150                 155                 160           

gcc cga aag gga ttc tgg ttc tcc cac ttt ggc tgg atg ctg ctt gtg        528
Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val           
                165                 170                 175               

ccc aac ccc aag aac aag ggc cga act gac att tct gac ctc aac aac        576
Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn           
            180                 185                 190                   

gac tgg gtt gtc cga ctc cag cac aag tac tac gtt tac gtt ctc gtc        624
Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val           
        195                 200                 205                       

ttc atg gcc att gtt ctg ccc acc ctc gtc tgt ggc ttt ggc tgg ggc        672
Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly           
    210                 215                 220                           

gac tgg aag gga ggt ctt gtc tac gcc ggt atc atg cga tac acc ttt        720
Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe           
225                 230                 235                 240           

gtg cag cag gtg act ttc tgt gtc aac tcc ctt gcc cac tgg att gga        768
Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly           
                245                 250                 255               

gag cag ccc ttc gac gac cga cga act ccc cga gac cac gct ctt acc        816
Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr           
            260                 265                 270                   

gcc ctg gtc acc ttt gga gag ggc tac cac aac ttc cac cac gag ttc        864
Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe           
        275                 280                 285                       

ccc tcg gac tac cga aac gcc ctc atc tgg tac cag tac gac ccc acc        912
Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr           
    290                 295                 300                           

aag tgg ctc atc tgg acc ctc aag cag gtt ggt ctc gcc tgg gac ctc        960
Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu           
305                 310                 315                 320           

cag acc ttc tcc cag aac gcc atc gag cag ggt ctc gtg cag cag cga       1008
Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg           
                325                 330                 335               

cag aag aag ctg gac aag tgg cga aac aac ctc aac tgg ggt atc ccc       1056
Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro           
            340                 345                 350                   

att gag cag ctg cct gtc att gag ttt gag gag ttc caa gag cag gcc       1104
Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala           
        355                 360                 365                       

aag acc cga gat ctg gtt ctc att tct ggc att gtc cac gac gtg tct       1152
Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser           
    370                 375                 380                           

gcc ttt gtc gag cac cac cct ggt gga aag gcc ctc att atg agc gcc       1200
Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala           
385                 390                 395                 400           

gtc ggc aag gac ggt acc gct gtc ttc aac gga ggt gtc tac cga cac       1248
Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His           
                405                 410                 415               

tcc aac gct ggc cac aac ctg ctt gcc acc atg cga gtt tcg gtc att       1296
Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile           
            420                 425                 430                   

cga ggc ggc atg gag gtt gag gtg tgg aag act gcc cag aac gaa aag       1344
Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys           
        435                 440                 445                       

aag gac cag aac att gtc tcc gat gag agt gga aac cga atc cac cga       1392
Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg           
    450                 455                 460                           

gct ggt ctc cag gcc acc cgg gtc gag aac ccc ggt atg tct ggc atg       1440
Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met           
465                 470                 475                 480           

gct gct tag                                                           1449
Ala Ala                                                                   
                                                                          


<210>  81
<211>  482
<212>  PRT
<213>  Yarrowia lipolytica

<400>  81

Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile 
1               5                   10                  15      


Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val 
            20                  25                  30          


Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu 
        35                  40                  45              


Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn 
    50                  55                  60                  


Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro 
65                  70                  75                  80  


Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr 
                85                  90                  95      


Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala 
            100                 105                 110         


His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu 
        115                 120                 125             


Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser 
    130                 135                 140                 


His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp 
145                 150                 155                 160 


Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val 
                165                 170                 175     


Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn 
            180                 185                 190         


Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val 
        195                 200                 205             


Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly 
    210                 215                 220                 


Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe 
225                 230                 235                 240 


Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly 
                245                 250                 255     


Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr 
            260                 265                 270         


Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe 
        275                 280                 285             


Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr 
    290                 295                 300                 


Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu 
305                 310                 315                 320 


Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg 
                325                 330                 335     


Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro 
            340                 345                 350         


Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala 
        355                 360                 365             


Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser 
    370                 375                 380                 


Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala 
385                 390                 395                 400 


Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His 
                405                 410                 415     


Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile 
            420                 425                 430         


Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys 
        435                 440                 445             


Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg 
    450                 455                 460                 


Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met 
465                 470                 475                 480 


Ala Ala 
        


<210>  82
<211>  4313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUM

<400>  82
taatcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc       60

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga      120

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg      180

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg      240

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg      300

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga      360

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg      420

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag      480

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc      540

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg      600

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt      660

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc      720

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc      780

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg      840

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca      900

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc      960

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     1020

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     1080

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     1140

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     1200

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     1260

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     1320

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     1380

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     1440

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     1500

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     1560

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     1620

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     1680

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     1740

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     1800

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     1860

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     1920

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     1980

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     2040

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     2100

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     2160

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     2220

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     2280

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     2340

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     2400

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     2460

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     2520

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     2580

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc     2640

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc     2700

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc     2760

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat     2820

tgggtaccgg gccccccctc gaggtcgacg agtatctgtc tgactcgtca ttgccgcctt     2880

tggagtacga ctccaactat gagtgtgctt ggatcacttt gacgatacat tcttcgttgg     2940

aggctgtggg tctgacagct gcgttttcgg cgcggttggc cgacaacaat atcagctgca     3000

acgtcattgc tggctttcat catgatcaca tttttgtcgg caaaggcgac gcccagagag     3060

ccattgacgt tctttctaat ttggaccgat agccgtatag tccagtctat ctataagttc     3120

aactaactcg taactattac cataacatat acttcactgc cccagataag gttccgataa     3180

aaagttctgc agactaaatt tatttcagtc tcctcttcac caccaaaatg ccctcctacg     3240

aagctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg     3300

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga     3360

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca     3420

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg     3480

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca     3540

acgcccacgg tgtacccgga accggaatcg attgctggcc tgcgagctgg tgcgtacgag     3600

gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc ccagtacaag     3660

gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat gctggccgag     3720

ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat tgagcttgcc     3780

cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa gggcgactct     3840

gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga cgctctcgga     3900

cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat aattgtcggc     3960

cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata ccagaaggct     4020

ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata tgtaatttaa     4080

ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg atggtcagac     4140

gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat gatctgtcca     4200

atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct aatacgttga     4260

actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt aat            4313


<210>  83
<211>  13565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL3-9DP9N

<400>  83
gtacggattg tgtatgtccc tgtacctgca tcttgatgga gagagctccg gaaagcggat       60

caggagctgt ccaattttaa ttttataaca tggaaacgag tccttggagc tagaagacca      120

ttttttcaac tgccctatcg actatattta tctactccaa aaccgactgc ttcccaagaa      180

tcttcagcca aggcttccaa agtaacccct cgcttcccga cacttaattg aaaccttaga      240

tgcagtcact gcgagtgaag tggactctaa catctccaac atagcgacga tattgcgagg      300

gtttgaatat aactaagatg catgatccat tacatttgta gaaatatcat aaacaacgaa      360

gcacatagac agaatgctgt tggttgttac atctgaagcc gaggtaccga tgtcattttc      420

agctgtcact gcagagacag gggtatgtca catttgaaga tcatacaacc gacgtttatg      480

aaaaccagag atatagagaa tgtattgacg gttgtggcta tgtcataagt gcagtgaagt      540

gcagtgatta taggtatagt acacttactg tagctacaag tacatactgc tacagtaata      600

ctcatgtatg caaaccgtat tctgtgtcta cagaaggcga tacggaagag tcaatctctt      660

atgtagagcc atttctataa tcgaaggggc cttgtaattt ccaaacgagt aattgagtaa      720

ttgaagagca tcgtagacat tacttatcat gtattgtgag agggaggaga tgcagctgta      780

gctactgcac atactgtact cgcccatgca gggataatgc atagcgagac ttggcagtag      840

gtgacagttg ctagctgcta cttgtagtcg ggtgggtgat agcatggcgc gccagctgca      900

ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc      960

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     1020

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     1080

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     1140

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     1200

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     1260

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     1320

ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     1380

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     1440

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     1500

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     1560

ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     1620

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     1680

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     1740

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     1800

atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta     1860

aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat     1920

ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac     1980

tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg     2040

ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag     2100

tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt     2160

aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt     2220

gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt     2280

tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt     2340

cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct     2400

tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt     2460

ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac     2520

cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa     2580

actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa     2640

ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca     2700

aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct     2760

ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga     2820

atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc     2880

tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt     2940

aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa     3000

ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt     3060

gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa     3120

agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag     3180

ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt     3240

tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg     3300

agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc     3360

cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg     3420

gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct     3480

gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg     3540

gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgcagga     3600

atagacatct tcaataggag cattaatacc tgtgggatca ctgatgtaaa cttctcccag     3660

agtatgtgaa taaccagcgg gccatccaac aaagaagtcg ttccagtgag tgactcggta     3720

catccgtctt tcggggttga tggtaagtcc gtcgtctcct tgcttaaaga acagagcgtc     3780

cacgtagtct gcaaaagcct tgtttccaag tcgaggctgc ccatagttga ttagcgttgg     3840

atcatatcca agattcttca ggttgatgcc catgaataga gcagtgacag ctcctagaga     3900

gtggccagtt acgatcaatt tgtagtcagt gttgtttcca aggaagtcga ccagacgatc     3960

ctgtacgttc accatagtct ctctgtatgc cttctgaaag ccatcatgaa cttggcagcc     4020

aggacaattg atactggcag aagggtttgt ggagtttatg tcagtagtgt taagaggagg     4080

gatactggtc atgtagggtt gttggatcgt ttggatgtca gtaatagcgt ctgcaatgga     4140

gaaagtgcct cggaaaacaa tatacttttc ctttttggtg tgatcgtggg ccaaaaatcc     4200

agtaactgaa gtcgagaaga aatttcctcc aaactggtag tcaagagtca catcgggaaa     4260

atgagcgcaa gagtttccac aggtaaaatc gctctgcagg gcaaatgggc caggggctct     4320

gacacaatag gccacgttag atagccatcc gtacttgaga acaaagtcgt atgtctcctg     4380

ggtgatagga gccgttaatt aagttgcgac acatgtcttg atagtatctt gaattctctc     4440

tcttgagctt ttccataaca agttcttctg cctccaggaa gtccatgggt ggtttgatca     4500

tggttttggt gtagtggtag tgcagtggtg gtattgtgac tggggatgta gttgagaata     4560

agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt caacgtatta     4620

gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca     4680

tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc     4740

atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat     4800

atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt     4860

atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg ccgacaatta     4920

tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt ccgagagcgt     4980

ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct     5040

taggtcggtt ctgggcaatg aagccaacca caaactcggg gtcggatcgg gcaagctcaa     5100

tggtctgctt ggagtactcg ccagtggcca gagagccctt gcaagacagc tcggccagca     5160

tgagcagacc tctggccagc ttctcgttgg gagaggggac taggaactcc ttgtactggg     5220

agttctcgta gtcagagacg tcctccttct tctgttcaga gacagtttcc tcggcaccag     5280

ctcgcaggcc agcaatgatt ccggttccgg gtacaccgtg ggcgttggtg atatcggacc     5340

actcggcgat tcggtgacac cggtactggt gcttgacagt gttgccaata tctgcgaact     5400

ttctgtcctc gaacaggaag aaaccgtgct taagagcaag ttccttgagg gggagcacag     5460

tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt tttgatcatg cacacataag     5520

gtccgacctt atcggcaagc tcaatgagct ccttggtggt ggtaacatcc agagaagcac     5580

acaggttggt tttcttggct gccacgagct tgagcactcg agcggcaaag gcggacttgt     5640

ggacgttagc tcgagcttcg taggagggca ttttggtggt gaagaggaga ctgaaataaa     5700

tttagtctgc agaacttttt atcggaacct tatctggggc agtgaagtat atgttatggt     5760

aatagttacg agttagttga acttatagat agactggact atacggctat cggtccaaat     5820

tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat     5880

gaaagccagc aatgacgttg cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag     5940

ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa agtgatccaa gcacactcat     6000

agttggagtc gtactccaaa ggcggcaatg acgagtcaga cagatactcg tcgacctttt     6060

ccttgggaac caccaccgtc agcccttctg actcacgtat tgtagccacc gacacaggca     6120

acagtccgtg gatagcagaa tatgtcttgt cggtccattt ctcaccaact ttaggcgtca     6180

agtgaatgtt gcagaagaag tatgtgcctt cattgagaat cggtgttgct gatttcaata     6240

aagtcttgag atcagtttgg ccagtcatgt tgtggggggt aattggattg agttatcgcc     6300

tacagtctgt acaggtatac tcgctgccca ctttatactt tttgattccg ctgcacttga     6360

agcaatgtcg tttaccaaaa gtgagaatgc tccacagaac acaccccagg gtatggttga     6420

gcaaaaaata aacactccga tacggggaat cgaaccccgg tctccacggt tctcaagaag     6480

tattcttgat gagagcgtat cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt     6540

tggcgctcat tgttgcgtta tgcagcgtac accacaatat tggaagctta ttagcctttc     6600

tattttttcg tttgcaaggc ttaacaacat tgctgtggag agggatgggg atatggaggc     6660

cgctggaggg agtcggagag gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa     6720

cgcacctagg accctttggc acgccgaaat gtgccacttt tcagtctagt aacgccttac     6780

ctacgtcatt ccatgcgtgc atgtttgcgc cttttttccc ttgcccttga tcgccacaca     6840

gtacagtgca ctgtacagtg gaggttttgg gggggtctta gatgggagct aaaagcggcc     6900

tagcggtaca ctagtgggat tgtatggagt ggcatggagc ctaggtggag cctgacagga     6960

cgcacgaccg gctagcccgt gacagacgat gggtggctcc tgttgtccac cgcgtacaaa     7020

tgtttgggcc aaagtcttgt cagccttgct tgcgaaccta attcccaatt ttgtcacttc     7080

gcacccccat tgatcgagcc ctaacccctg cccatcaggc aatccaatta agctcgcatt     7140

gtctgccttg tttagtttgg ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca     7200

agcattatat ataaggctcg tctctccctc ccaaccacac tcactttttt gcccgtcttc     7260

ccttgctaac acaaaagtca agaacacaaa caaccacccc aaccccctta cacacaagac     7320

atatctacag caatggccat ggccaaaagc aaacgacggt cggaggctgt ggaagagcac     7380

gtgaccggct cggacgaggg cttgaccgat acttcgggtc acgtgagccc tgccgccaag     7440

aagcagaaga actcggagat tcatttcacc acccaggctg cccagcagtt ggatcgggag     7500

cgcaaggagg agtatctgga ctcgctgatc gacaacaagg actatctcaa gtaccgtcct     7560

cgaggctgga agctcaacaa cccgcctacc gaccgacctg tgcgaatcta cgccgatgga     7620

gtgtttgatt tgttccatct gggacacatg cgtcagctgg agcagtccaa gaaggccttc     7680

cccaacgcag tgttgattgt gggcattccc agcgacaagg agacccacaa gcggaaggga     7740

ttgaccgtgc tgagtgacgt ccagcggtac gagacggtgc gacactgcaa gtgggtggac     7800

gaggtggtgg aggatgctcc ctggtgtgtc accatggact ttctggaaaa acacaaaatc     7860

gactacgtgg cccatgacga tctgccctac gcttccggca acgacgatga tatctacaag     7920

cccatcaagg agaagggcat gtttctggcc acccagcgaa ccgagggcat ttccacctcg     7980

gacatcatca ccaagattat ccgagactac gacaagtatt taatgcgaaa ctttgcccgg     8040

ggtgctaacc gaaaggatct caacgtctcg tggctcaaga agaacgagct ggacttcaag     8100

cgtcatgtgg ccgagttccg aaactcgttc aagcgaaaga aggtcggtaa ggatctctac     8160

ggcgagattc gcggtctgct gcagaatgtg ctcatttgga acggcgacaa ctccggcact     8220

tccactcccc agcgaaagac gctgcagacc aacgccaaga agatgtacat gaacgtgctc     8280

aagactctgc aggctcctga cgctgttgac gtggactcct cggagaacgt gtctgagaac     8340

gtcactgatg aggaggagga agacgacgac gaggttgatg aggacgaaga agccgacgac     8400

gacgacgaag acgacgaaga cgaggaagac gacgagtagg cggccgcatt gatgattgga     8460

aacacacaca tgggttatat ctaggtgaga gttagttgga cagttatata ttaaatcagc     8520

tatgccaacg gtaacttcat tcatgtcaac gaggaaccag tgactgcaag taatatagaa     8580

tttgaccacc ttgccattct cttgcactcc tttactatat ctcatttatt tcttatatac     8640

aaatcacttc ttcttcccag catcgagctc ggaaacctca tgagcaataa catcgtggat     8700

ctcgtcaata gagggctttt tggactcctt gctgttggcc accttgtcct tgctgtttaa     8760

acacgcagta ggatgtcctg cacgggtctt tttgtggggt gtggagaaag gggtgcttgg     8820

agatggaagc cggtagaacc gggctgcttg tgcttggaga tggaagccgg tagaaccggg     8880

ctgcttgggg ggatttgggg ccgctgggct ccaaagaggg gtaggcattt cgttggggtt     8940

acgtaattgc ggcatttggg tcctgcgcgc atgtcccatt ggtcagaatt agtccggata     9000

ggagacttat cagccaatca cagcgccgga tccacctgta ggttgggttg ggtgggagca     9060

cccctccaca gagtagagtc aaacagcagc agcaacatga tagttggggg tgtgcgtgtt     9120

aaaggaaaaa aaagaagctt gggttatatt cccgctctat ttagaggttg cgggatagac     9180

gccgacggag ggcaatggcg ctatggaacc ttgcggatat ccatacgccg cggcggactg     9240

cgtccgaacc agctccagca gcgttttttc cgggccattg agccgactgc gaccccgcca     9300

acgtgtcttg gcccacgcac tcatgtcatg ttggtgttgg gaggccactt tttaagtagc     9360

acaaggcacc tagctcgcag caaggtgtcc gaaccaaaga agcggctgca gtggtgcaaa     9420

cggggcggaa acggcgggaa aaagccacgg gggcacgaat tgaggcacgc cctcgaattt     9480

gagacgagtc acggccccat tcgcccgcgc aatggctcgc caacgcccgg tcttttgcac     9540

cacatcaggt taccccaagc caaacctttg tgttaaaaag cttaacatat tataccgaac     9600

gtaggtttgg gcgggcttgc tccgtctgtc caaggcaaca tttatataag ggtctgcatc     9660

gccggctcaa ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca     9720

cacatcaaca tggccatcaa agtcggtatt aacggattcg ggcgaatcgg acgaattgtg     9780

agtaccatag aaggtgatgg aaacatgacc caacagaaac agatgacaag tgtcatcgac     9840

ccaccagagc ccaattgagc tcatactaac agtcgacaac ctgtcgaacc aattgatgac     9900

tccccgacaa tgtactaaca caggtcctgc ccatggtgaa aaacgtggac caagtggatc     9960

tctcgcaggt cgacaccatt gcctccggcc gagatgtcaa ctacaaggtc aagtacacct    10020

ccggcgttaa gatgagccag ggcgcctacg acgacaaggg ccgccacatt tccgagcagc    10080

ccttcacctg ggccaactgg caccagcaca tcaactggct caacttcatt ctggtgattg    10140

cgctgcctct gtcgtccttt gctgccgctc ccttcgtctc cttcaactgg aagaccgccg    10200

cgtttgctgt cggctattac atgtgcaccg gtctcggtat caccgccggc taccaccgaa    10260

tgtgggccca tcgagcctac aaggccgctc tgcccgttcg aatcatcctt gctctgtttg    10320

gaggaggagc tgtcgagggc tccatccgat ggtgggcctc gtctcaccga gtccaccacc    10380

gatggaccga ctccaacaag gacccttacg acgcccgaaa gggattctgg ttctcccact    10440

ttggctggat gctgcttgtg cccaacccca agaacaaggg ccgaactgac atttctgacc    10500

tcaacaacga ctgggttgtc cgactccagc acaagtacta cgtttacgtt ctcgtcttca    10560

tggccattgt tctgcccacc ctcgtctgtg gctttggctg gggcgactgg aagggaggtc    10620

ttgtctacgc cggtatcatg cgatacacct ttgtgcagca ggtgactttc tgtgtcaact    10680

cccttgccca ctggattgga gagcagccct tcgacgaccg acgaactccc cgagaccacg    10740

ctcttaccgc cctggtcacc tttggagagg gctaccacaa cttccaccac gagttcccct    10800

cggactaccg aaacgccctc atctggtacc agtacgaccc caccaagtgg ctcatctgga    10860

ccctcaagca ggttggtctc gcctgggacc tccagacctt ctcccagaac gccatcgagc    10920

agggtctcgt gcagcagcga cagaagaagc tggacaagtg gcgaaacaac ctcaactggg    10980

gtatccccat tgagcagctg cctgtcattg agtttgagga gttccaagag caggccaaga    11040

cccgagatct ggttctcatt tctggcattg tccacgacgt gtctgccttt gtcgagcacc    11100

accctggtgg aaaggccctc attatgagcg ccgtcggcaa ggacggtacc gctgtcttca    11160

acggaggtgt ctaccgacac tccaacgctg gccacaacct gcttgccacc atgcgagttt    11220

cggtcattcg aggcggcatg gaggttgagg tgtggaagac tgcccagaac gaaaagaagg    11280

accagaacat tgtctccgat gagagtggaa accgaatcca ccgagctggt ctccaggcca    11340

cccgggtcga gaaccccggt atgtctggca tggctgctta ggcggccgca tgagaagata    11400

aatatataaa tacattgaga tattaaatgc gctagattag agagcctcat actgctcgga    11460

gagaagccaa gacgagtact caaaggggat tacaccatcc atatccacag acacaagctg    11520

gggaaaggtt ctatatacac tttccggaat accgtagttt ccgatgttat caatgggggc    11580

agccaggatt tcaggcactt cggtgtctcg gggtgaaatg gcgttcttgg cctccatcaa    11640

gtcgtaccat gtcttcattt gcctgtcaaa gtaaaacaga agcagatgaa gaatgaactt    11700

gaagtgaagg aatttaaata gttggagcaa gggagaaatg tagagtgtga aagactcact    11760

atggtccggg cttatctcga ccaatagcca aagtctggag tttctgagag aaaaaggcaa    11820

gatacgtatg taacaaagcg acgcatggta caataatacc ggaggcatgt atcatagaga    11880

gttagtggtt cgatgatggc actggtgcct ggtatgactt tatacggctg actacatatt    11940

tgtcctcaga catacaatta cagtcaagca cttacccttg gacatctgta ggtacccccc    12000

ggccaagacg atctcagcgt gtcgtatgtc ggattggcgt agctccctcg ctcgtcaatt    12060

ggctcccatc tactttcttc tgcttggcta cacccagcat gtctgctatg gctcgttttc    12120

gtgccttatc tatcctccca gtattaccaa ctctaaatga catgatgtga ttgggtctac    12180

actttcatat cagagataag gagtagcaca gttgcataaa aagcccaact ctaatcagct    12240

tcttcctttc ttgtaattag tacaaaggtg attagcgaaa tctggaagct tagttggccc    12300

taaaaaaatc aaaaaaagca aaaaacgaaa aacgaaaaac cacagttttg agaacaggga    12360

ggtaacgaag gatcgtatat atatatatat atatatatac ccacggatcc cgagaccggc    12420

ctttgattct tccctacaac caaccattct caccacccta attcacaacc atggaggtcg    12480

tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat gctcagctct    12540

ggtctgatgc ctcgcactgc gaggtgctgt acctctccat cgccttcgtc atcctgaagt    12600

tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg ttcaccaact    12660

acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc tacgccatgt    12720

acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat gtcttccgaa    12780

tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc ttctatctgc    12840

ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga gctcctatgg    12900

acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg ctgctcaacg    12960

gcttcattca ctggatcatg tacggctact attggacccg actgatcaag ctcaagttcc    13020

ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt ggcttctaca    13080

tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga atgtttggct    13140

ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac ttctacgtgc    13200

agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtgagcg gccgcaagtg    13260

tggatgggga agtgagtgcc cggttctgtg tgcacaattg gcaatccaag atggatggat    13320

tcaacacagg gatatagcga gctacgtggt ggtgcgagga tatagcaacg gatatttatg    13380

tttgacactt gagaatgtac gatacaagca ctgtccaagt acaatactaa acatactgta    13440

catactcata ctcgtacccg gcaacggttt cacttgagtg cagtggctag tgctcttact    13500

cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta    13560

gttgc                                                                13565


<210>  84
<211>  9632
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pY187

<400>  84
ggccgcatga gaagataaat atataaatac attgagatat taaatgcgct agattagaga       60

gcctcatact gctcggagag aagccaagac gagtactcaa aggggattac accatccata      120

tccacagaca caagctgggg aaaggttcta tatacacttt ccggaatacc gtagtttccg      180

atgttatcaa tgggggcagc caggatttca ggcacttcgg tgtctcgggg tgaaatggcg      240

ttcttggcct ccatcaagtc gtaccatgtc ttcatttgcc tgtcaaagta aaacagaagc      300

agatgaagaa tgaacttgaa gtgaaggaat ttaaatgtaa cgaaactgaa atttgaccag      360

atattgtgtc cgcggtggag ctccagcttt tgttcccttt agtgagggtt aatttcgagc      420

ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaagcttc      480

cacacaacgt acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct      540

aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc      600

agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt      660

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag      720

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca      780

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt      840

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc      900

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct      960

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     1020

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     1080

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     1140

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     1200

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     1260

actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct     1320

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     1380

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     1440

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     1500

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     1560

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     1620

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     1680

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     1740

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     1800

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     1860

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca     1920

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     1980

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     2040

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     2100

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     2160

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg     2220

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     2280

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     2340

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     2400

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     2460

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     2520

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     2580

tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca     2640

gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct     2700

ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt     2760

tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac     2820

gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct     2880

ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt     2940

ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac     3000

aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag     3060

gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc     3120

gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg     3180

acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggtac     3240

cgggccctcc ttcacttcaa gttcattctt catctgcttc tgttttactt tgacaggcaa     3300

atgaagacat ggtacgactt gatggaggcc aagaacgcca tttcaccccg agacaccgaa     3360

gtgcctgaaa tcctggctgc ccccattgat aacatcggaa actacggtat tccggaaagt     3420

gtatatagaa cctttcccca gcttgtgtct gtggatatgg atggtgtaat cccctttgag     3480

tactcgtctt ggcttctctc cgagcagtat gaggctctct aatctagcgc atttaatatc     3540

tcaatgtatt tatatattta tcttctcatg cggccgccta ctgagtcttc tggccagcgt     3600

agccaatctg gtcggcagtc tcgcacatgg ccttataagt ggtggccatg agagcgtcga     3660

cgttgctggc gtccagaccc ttggtctcaa tggggctcag gacgtcgaca aggatctctc     3720

cggcgtccag cttgcctcgg ccgaaagaaa acattcggga ggtgttctgg accaccacgg     3780

ggacaatggg agcgcccgac tggaccgcca ggtggaaaca gcccttcttg aagggcatga     3840

tgccgacgtc cttggagtag gatcgggttc cctcggggaa catgaagacg ctctggccct     3900

tgcctccgtt gccgctctgg atggtcttga cggcgccgcc gagggtctgc acggacttgg     3960

ttcggtcctt tcggtccagg aagatggtgc cggacagcgc catgaactgg cccagcagag     4020

ggtaccactt gagggccttt ttggcggtga cggagcagta ctgggggaag atgcgaccga     4080

gcaccagaat gtccatttcg ttctggtggt tggaaatcag aatgcatggt cggggcaggt     4140

gtttggtgat gtcgtcaatg ggcttggtgg tgttggcgac ctggatcttg gaggcatcca     4200

gaccggcggt tccggtcacc tgccggctac gcagcttgat gctgataccc aggaagatgc     4260

gcaccgagta gtagaaggct ctggcaacgg tccattgggc caggccctgc ttgccgatgg     4320

cggtgagaat ggtggacgcc agcacgccgt aggtggcaca ggcggcaaag atgaccacgg     4380

cgatggcggc gcggacgtag aagacgagct tggatgcaac ggacatgagc tgggttagtt     4440

tgtgtagaga gtgtgtgttg ctagcgactt tcggattgtg tcattacaca aaacgcgtcg     4500

tctcgacact gatcttgtcg tggatactca cggctcggaa ttctgtgatg tgtagtttag     4560

atttcgaatc tgtggggaaa gaaaggaaaa aagagactgg caaccgattg ggagagccac     4620

tgtttatata taccctagac aagccccccg cttgtaagat gttggtcaat gtaaaccagt     4680

attaaggttg gcaagtgcag gagaagcaag gtgtgggtac cgagcaatgg aaatgtgcgg     4740

aaggcaaaaa aatgaggcca cggcctattg tcggggctat atccaggggg cgattgaagt     4800

acactaacat gacatgtgtc cacagaccct caatctggcc tgatgagcca aatccatacg     4860

cgctttcgca gctctaaagg ctataacaag tcacaccacc ctgctcgacc tcagcgccct     4920

cactttttgt taagacaaac tgtacacgct gttccagcgt tttctgcctg cacctggtgg     4980

gacatttggt gcaacctaaa gtgctcggaa cctctgtggt gtccagatca gcgcagcagt     5040

tccgaggtag ttttgaggcc cttagatgat gcaatggtgt cagtcgctgg atcacgagtc     5100

ttaatggcag tattcgttct tatttgtgcc attgagcccc gttatcctcg tatcttctac     5160

cccccatccc atccctttgt tggtgcaacc ctacccattt attgttgggt gcagcccaac     5220

cgacgtggag agcttggctt ggccatataa aaaggccccc ccctagtggc aatggcagaa     5280

agtcagctgt gagttgttga atttgtcatc taggcggcct ggccgtcttc tgcatgctac     5340

ttgggtgtaa tattgggatc tgttcggaaa tcaacggatg ctcaatcgat ttcgacagta     5400

attaattaag tcatacacaa gtcagctttc ttcgagcctc atataagtat aagtagttca     5460

acgtattagc actgtaccca gcatctccgt atcgagaaac acaacaacat gccccattgg     5520

acagatcatg cggatacaca ggttgtgcag tatcatacat actcgatcag acaggtcgtc     5580

tgaccatcat acaagctgaa caagcgctcc atacttgcac gctctctata tacacagtta     5640

aattacatat ccatagtcta acctctaaca gttaatcttc tggtaagcct cccagccagc     5700

cttctggtat cgcttggcct cctcaatagg atctcggttc tggccgtaca gacctcggcc     5760

gacaattatg atatccgttc cggtagacat gacatcctca acagttcggt actgctgtcc     5820

gagagcgtct cccttgtcgt caagacccac cccgggggtc agaataagcc agtcctcaga     5880

gtcgccctta ggtcggttct gggcaatgaa gccaaccaca aactcggggt cggatcgggc     5940

aagctcaatg gtctgcttgg agtactcgcc agtggccaga gagcccttgc aagacagctc     6000

ggccagcatg agcagacctc tggccagctt ctcgttggga gaggggacta ggaactcctt     6060

gtactgggag ttctcgtagt cagagacgtc ctccttcttc tgttcagaga cagtttcctc     6120

ggcaccagct cgcaggccag caatgattcc ggttccgggt acaccgtggg cgttggtgat     6180

atcggaccac tcggcgattc ggtgacaccg gtactggtgc ttgacagtgt tgccaatatc     6240

tgcgaacttt ctgtcctcga acaggaagaa accgtgctta agagcaagtt ccttgagggg     6300

gagcacagtg ccggcgtagg tgaagtcgtc aatgatgtcg atatgggttt tgatcatgca     6360

cacataaggt ccgaccttat cggcaagctc aatgagctcc ttggtggtgg taacatccag     6420

agaagcacac aggttggttt tcttggctgc cacgagcttg agcactcgag cggcaaaggc     6480

ggacttgtgg acgttagctc gagcttcgta ggagggcatt ttggtggtga agaggagact     6540

gaaataaatt tagtctgcag aactttttat cggaacctta tctggggcag tgaagtatat     6600

gttatggtaa tagttacgag ttagttgaac ttatagatag actggactat acggctatcg     6660

gtccaaatta gaaagaacgt caatggctct ctgggcgtcg cctttgccga caaaaatgtg     6720

atcatgatga aagccagcaa tgacgttgca gctgatattg ttgtcggcca accgcgccga     6780

aaacgcagct gtcagaccca cagcctccaa cgaagaatgt atcgtcaaag tgatccaagc     6840

acactcatag ttggagtcgt actccaaagg cggcaatgac gagtcagaca gatactcgtc     6900

gaccgtacga tagttagtag acaacaatcg atagttggag caagggagaa atgtagagtg     6960

tgaaagactc actatggtcc gggcttatct cgaccaatag ccaaagtctg gagtttctga     7020

gagaaaaagg caagatacgt atgtaacaaa gcgacgcatg gtacaataat accggaggca     7080

tgtatcatag agagttagtg gttcgatgat ggcactggtg cctggtatga ctttatacgg     7140

ctgactacat atttgtcctc agacatacaa ttacagtcaa gcacttaccc ttggacatct     7200

gtaggtaccc cccggccaag acgatctcag cgtgtcgtat gtcggattgg cgtagctccc     7260

tcgctcgtca attggctccc atctactttc ttctgcttgg ctacacccag catgtctgct     7320

atggctcgtt ttcgtgcctt atctatcctc ccagtattac caactctaaa tgacatgatg     7380

tgattgggtc tacactttca tatcagagat aaggagtagc acagttgcat aaaaagccca     7440

actctaatca gcttcttcct ttcttgtaat tagtacaaag gtgattagcg aaatctggaa     7500

gcttagttgg ccctaaaaaa atcaaaaaaa gcaaaaaacg aaaaacgaaa aaccacagtt     7560

ttgagaacag ggaggtaacg aaggatcgta tatatatata tatatatata tacccacgga     7620

tcccgagacc ggcctttgat tcttccctac aaccaaccat tctcaccacc ctaattcaca     7680

accatgacac aacctgtgaa tcggaaggcg actgtcgagc gggtcgagcc agcagtggag     7740

gtggctgact ccgagtccga ggccaagacc gacgtccacg ttcaccacca tcatcaccac     7800

cacaagcgaa aatccgtcaa gggcaagatt ctcaacttct tcacccgaag tcgacgtatc     7860

accttcgtcc tcggcgccgt ggtcggtgtg atagccgcgg gatactacgc tgcgccaccg     7920

gagctcagca ttgatatcga tgctcttctc ggcgacttgc cctcgttcga ctttgacgct     7980

ctatctctcg acaacttgtc catggacagt gtgtcggact ttgtacaaga catgaaatcg     8040

cggtttccga ccaagattct gcaggaggcg gccaagatcg agaagcacca gaaaagcgaa     8100

cagaaggctg ccccttttgc tgtgggcaag gctatgaaga gcgagggact caacgccaag     8160

tacccggtgg tgctggtgcc cggcgtcatc tccacgggac tggagagctg gtccctggag     8220

ggaaccgagg agtgtcccac cgagtcgcac ttcagaaagc gaatgtgggg ctcctggtac     8280

atgatccgag tcatgctgct ggacaagtac tgctggctgc agaacctgat gctggacaca     8340

gagaccggtc tagaccctcc ccatttcaag ctgcgagccg cccagggatt tgcctccgcc     8400

gacttcttta tggcaggcta ctggctgtgg aacaagctgc tcgagaacct ggctgttatt     8460

ggatacgata cggatacaat gtctgctgcg gcgtacgact ggagactgtc ctaccctgat     8520

ttggagcacc gagacggata cttctccaag ctcaaagctt caatcgaaga gactaagcgt     8580

atgacaggtg agaagacagt tctgacgggc cattccatgg gctcccaggt catcttctac     8640

ttcatgaagt gggctgaggc cgagggatat ggaggaggag gtcccaactg ggtcaatgac     8700

catattgaat cctttgtcga catttccggc tccatgctgg gtactcccaa gaccctggtt     8760

gctcttctgt ctggagaaat gaaggatacc gtgcagctga acgcgatggc tgtgtatgga     8820

ctggagcagt tcttctctcg acgagagcga gccgatctgc tgcgaacatg gggaggaatt     8880

gcttccatga ttcccaaggg tggtaaggct atctggggtg atcattctgg agcccctgat     8940

gacgagcccg gccagaatgt cacctttggc aacttcatca agttcaagga gtccttgacc     9000

gagtactctg ctaagaacct caccatggat gaaaccgttg acttcctgta ttctcagtct     9060

cccgagtggt ttgtgaaccg aaccgagggt gcttactcct ttggaattgc caagactcga     9120

aagcaggttg agcagaatga gaagcgacct tctacctgga gcaaccctct ggaagctgct     9180

ctccccaatg cccccgatct caagatctac tgcttctatg gagtcggtaa ggataccgag     9240

cgagcctact actaccagga tgagcccaat cccgagcaga ccaacttgaa cgtcagtatc     9300

gctggaaacg accctgatgg tgtgcttatg ggtcagggcg atggaaccgt ctcccttgtg     9360

acccatacca tgtgtcaccg atggaaggac gagaattcca agttcaaccc tggtaacgcc     9420

caggtcaagg ttgtggagat gttgcaccag cctgatcgac ttgatattcg aggcggtgct     9480

cagactgccg agcatgtgga cattctgggg cgttctgagt tgaacgagat ggttctgaag     9540

gtggctagtg gaaagggaaa tgagattgaa gagagagtca tctccaacat tgatgagtgg     9600

gtgtggaaga ttgatctcgg cagcaattag gc                                   9632


<210>  85
<211>  15262
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZK16-ML8N

<400>  85
gtacgcattc ttgacttgct ccccagttgc cctttttggt cataactcag ccccgactct       60

ctccccaaag aggagatgga cttacaatag atacgagaca acagatattt tcggccacct      120

ggtgcaattg gtctgtttta ttacccccaa taatatccac agcgtccgaa tcagctcgac      180

aagctctttc tcagcatact gatcttaccc catacccccc agacagcggt gccatatcca      240

tacccgcact acctgagaca gtaaactcag ggcaaggagc ggtcttggtc catcgcgacg      300

ctgtaagctt tgcctttgcg ttttgtgatc caccacacgg cagtcaaggt ctcggttgaa      360

agatgggtct ggcaatgtgg attgctgaat cattgacata taaatacgag tgatcctgcc      420

atttcccaat ctttagaaat catcttttct tcacacatac aaaaaacaca catcacaatg      480

cagttctccg ccgccgccgt ccttgccctc gccgccttcg tctctgccca ggccgcctcc      540

gctgcttctg aagctggtgg tgccgttgtc tccggtgcct ccggtgccgc tgccgccgct      600

tcttctgctg tcattggctc cgagtctcag gccgcttctt ctgctgcctc ttctgctgct      660

gagtctgctt cttccgctgc ctctgagtcc gcctcctccg cttccgcctc cgagtccaag      720

gcctctgagt ccgcttccaa gtccgcctcc aaggcttctg agtctgcttc caaggccgag      780

tcttctgctg ccaaggcttc ctccgctgct ggttctgctt cttccgccgc tgcctctgcc      840

accaagtctg gtgatgcctc cggctcttcc aagccttccg gctctgcctc ctctaccggt      900

gacaagtccg gtgctggtat tgttgccccc gctgttggtg ccatcgttgt cggtgctctt      960

gctcagctca tctaagcggt ctttgtacaa ctacccactt aatatttata ttatgatata     1020

gactgcaata ctattgcatg tgtaatgctt cgtttgaaag tcgtagtccg gccattcatg     1080

gcagtgtatg gcagtattgc aaccaattgg cttaataaca tatacaaagt ggcaattctg     1140

tgttcgaacc atcacacaac ccaacatatt tgacattgct acaagtattc gacttgttct     1200

tcggccgcac gacatgcatt gctagcagca cacttgcaga agcttggcca atggttaggt     1260

tgacctttaa ccagtaaaac gcaatatagg ttgccaatta acaaacaaca cagcccagaa     1320

ctggtgtaaa gggcttgggt tttgtgcaat caactcattg actcacctcc cccaatgctc     1380

catgaaccca tgaatccata gtacctccat cccaattcag agaggctcag aaacacgcaa     1440

ttggcaaaag taaacaaagg tctcgaaacc ttaaggggtg cagtgttgca aagttaaaat     1500

tacactgttg gatggtgttc cactcaaact gtggatggat cagacggttg cactaaacca     1560

agattcaata tgctctacaa gtagcgtgcc atgaggtgta taatatcaca catgatcaga     1620

gttaaggcct ggaaaaaaag tgtccgcggc tatgtaacac tgctgaatac agtgtgtgcc     1680

gtttccacgg actggaatga atgcactcaa cacgttggta gctaacttcc ccaactattg     1740

acttcacttt actgtagcta ctctaagtac gataatgaca tcatgaatca ccaactttat     1800

cttccccacc gaaagtaaga gatgaaagag aggctaacag cttttgtaag aaaatcacaa     1860

aatctactta ccgtattaca aaagtacaag tatcagtaac caggcgcgcc agctgcatta     1920

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     1980

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     2040

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     2100

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     2160

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     2220

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     2280

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     2340

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     2400

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     2460

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     2520

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     2580

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     2640

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     2700

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     2760

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     2820

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     2880

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     2940

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     3000

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     3060

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     3120

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     3180

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc     3240

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     3300

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     3360

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     3420

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     3480

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc     3540

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     3600

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     3660

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     3720

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     3780

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     3840

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     3900

tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag     3960

cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca     4020

ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag     4080

tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg     4140

gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt     4200

tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag     4260

agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc     4320

gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc     4380

gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa     4440

gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca     4500

aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc     4560

agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca tgctatgtac     4620

actgtgtgag tgtagagggc aatggtggtg acaactgagc tactatacac acacagcttg     4680

tactggtata agttgtgagc tacagtacaa gtaatactac aaaattcagt atgtacaagt     4740

atgccggtac ggcagtagca tgtatcaaag agagacagaa agaaggctgg aaagcgaagg     4800

ccagctgagc gtgggaaccg cagtacgtgg aagtgccaaa cctgcgacag aatgacatgg     4860

tcttgcactt tttaccggta ccggtacagt acgataccgg tacgcacttg ctacagtaga     4920

tcatagacca ccagaataac cctgcattag cgccacgttg ccaagccctc aactttcttt     4980

gcagctacac cttttacacc ttttaggctg ccaggaagag cataaatgaa ttgtacttgg     5040

gggcacagaa tttgggttga agtgttcctg ggagagaatg tgcttggctg tatagttggg     5100

tggctctcct tcatagtaga tgtgaggtta cagcacttgt agattgtgta cgcgttctgt     5160

ttaaggtggt accagtatta tgggttgacg tgaaagaaag ggaggaggca ggggtacagc     5220

aagagtgtgg gcgcacactc acacatgaag caagacctgt ttcgtcattt ctatttgttt     5280

aacaaggacg acataatata cttaggacag catgggccga ccggcaggaa taatctgcaa     5340

acacaatcgt attgtggcgt cccctctcga tcacgatagt tgctaagttt agcagtctga     5400

gagtttggct gtgacgcaag accgagagag agacagagag ttgtcccagt ttgagtactc     5460

gtaccgtact acagagtaaa tcgaaatcga gcgctcactt tctgcacttt gtgaaggcgc     5520

agcattcaac atttgtgtcc agcccctttc cgtttgaagc gacttgacca cgaaatgttg     5580

gaataaagct gtgttacata cgagacctac aagtagagtg tcggatatgc caactgaaca     5640

ggttagatga aagttactca acaacaggag agcggacacc aacgtgcgaa aatgtacgga     5700

tgaacccatt gtctgtccgc atagcaatct gttttcgcca gcagtgcttg tgtcgttgga     5760

ttgctgccat tgtactattt gtactttgtt ccattaacac tcgcatcttg ctcaccgatt     5820

agcagcactc ctgttgcctc actgggcttg tctgtttcag ggtggaatgc atgagcaatg     5880

cgatcgtcaa ggggaggttg aaagcgcgta tggaataact gtggttctat agctgagggg     5940

aggatatcga ggacagccac ggttcagtgg gggatttcgg gagctactca gtaacatgta     6000

ctcttgtccg aacgccctgc tgatggcaaa tatgacaaaa cgcaacaatg tggagcaaca     6060

tttccatttc gagagatatt acatgggttg cattggggcc tgggcatccc gtgcaataac     6120

cgagaccatg tgaaggtgca cgatcgacac ttatccagcc agtcatacgt ggtgtcgcaa     6180

gctaagcaac aatgggattc acgaatctat gtgggggaac gtgcacaggg gaaatgaaac     6240

aaaacggagg agggggtctt tgaaaatgta gagaaatata acaaaaaatc acaaaaacgc     6300

accaaaaata tgccaaaatc gtcaacagtg gaaatcacgc gcccctggct cgacttccca     6360

ctgacatacc ccccctcaac caatatcata tccaccctag ccaatcgctt aattaagttg     6420

cgacacatgt cttgatagta tcttgaattc tctctcttga gcttttccat aacaagttct     6480

tctgcctcca ggaagtccat gggtggtttg atcatggttt tggtgtagtg gtagtgcagt     6540

ggtggtattg tgactgggga tgtagttgag aataagtcat acacaagtca gctttcttcg     6600

agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     6660

agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     6720

atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     6780

ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     6840

atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     6900

cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     6960

tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     7020

ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     7080

accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     7140

gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     7200

ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     7260

ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     7320

ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     7380

tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     7440

tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     7500

atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     7560

agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     7620

agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     7680

ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     7740

accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     7800

agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     7860

gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg     7920

atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa     7980

gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc     8040

aatgacgagt cagacagata ctcgtcgacc ttttccttgg gaaccaccac cgtcagccct     8100

tctgactcac gtattgtagc caccgacaca ggcaacagtc cgtggatagc agaatatgtc     8160

ttgtcggtcc atttctcacc aactttaggc gtcaagtgaa tgttgcagaa gaagtatgtg     8220

ccttcattga gaatcggtgt tgctgatttc aataaagtct tgagatcagt ttggccagtc     8280

atgttgtggg gggtaattgg attgagttat cgcctacagt ctgtacaggt atactcgctg     8340

cccactttat actttttgat tccgctgcac ttgaagcaat gtcgtttacc aaaagtgaga     8400

atgctccaca gaacacaccc cagggtatgg ttgagcaaaa aataaacact ccgatacggg     8460

gaatcgaacc ccggtctcca cggttctcaa gaagtattct tgatgagagc gtatcgatag     8520

ttggagcaag ggagaaatgt agagtgtgaa agactcacta tggtccgggc ttatctcgac     8580

caatagccaa agtctggagt ttctgagaga aaaaggcaag atacgtatgt aacaaagcga     8640

cgcatggtac aataataccg gaggcatgta tcatagagag ttagtggttc gatgatggca     8700

ctggtgcctg gtatgacttt atacggctga ctacatattt gtcctcagac atacaattac     8760

agtcaagcac ttacccttgg acatctgtag gtaccccccg gccaagacga tctcagcgtg     8820

tcgtatgtcg gattggcgta gctccctcgc tcgtcaattg gctcccatct actttcttct     8880

gcttggctac acccagcatg tctgctatgg ctcgttttcg tgccttatct atcctcccag     8940

tattaccaac tctaaatgac atgatgtgat tgggtctaca ctttcatatc agagataagg     9000

agtagcacag ttgcataaaa agcccaactc taatcagctt cttcctttct tgtaattagt     9060

acaaaggtga ttagcgaaat ctggaagctt agttggccct aaaaaaatca aaaaaagcaa     9120

aaaacgaaaa acgaaaaacc acagttttga gaacagggag gtaacgaagg atcgtatata     9180

tatatatata tatatatacc cacggatccc gagaccggcc tttgattctt ccctacaacc     9240

aaccattctc accaccctaa ttcacaacca tgtctattgg ttcgtccaac cccgtgctct     9300

tggctgcgat tcccttcgtc tacctgtttg tcctcccacg agtcctggct ttcctgcctc     9360

agaaggctca gttcctggcc aaatgtattg tggtcctgat tgccacgctt atcatgtccg     9420

ttgcaggctg cttcatctcg atcgtgtgcg ctcttctgga caagagatac gtcatcaatt     9480

acgttgtgtc gcgattgttc tccttccttg ccgctcgacc gtgtggtgtg acctataaga     9540

ttgttggtga ggaacacctc gataagtacc ctgctatcgt ggtctgtaac catcaatcct     9600

ctatggatat gatggttttg ggacgagttt ttccaaagca ctgcgttgtc atggcgaaga     9660

aggaactcct gtactttccc tttttgggaa tgtttatgaa actgagcaac gctatcttca     9720

tcgaccggaa gaaccacaag aaagccatcg agtctaccac ccaagccgtg gcggacatga     9780

agaagcacaa ctctggaatc tggattttcc cagagggcac ccggtctaga ctggacaagg     9840

cagacctgct gcccttcaag aaaggtgcct ttcatcttgc aattcaggcc cagctcccta     9900

ttctccccat tatctcgcag ggctattccc atatctacga ctcttcgaag cggtacttcc     9960

ccggtggaga gctcgagatc agagtcctgg agcccattcc tacaactggc ctcactactg    10020

atgatgtgaa cgacctgatg gacaagacac gaaaccttat gctcaagcac ttgaaggaga    10080

tggattccca gtattcgtcg agcactgctg aaaatggatc cacgcacatc gacgccgata    10140

ttgccaagtc tacagccacc agcattggca acactgacga cgcaattaca aaacgtcgta    10200

cccctaagga ataagcggcc gcattgatga ttggaaacac acacatgggt tatatctagg    10260

tgagagttag ttggacagtt atatattaaa tcagctatgc caacggtaac ttcattcatg    10320

tcaacgagga accagtgact gcaagtaata tagaatttga ccaccttgcc attctcttgc    10380

actcctttac tatatctcat ttatttctta tatacaaatc acttcttctt cccagcatcg    10440

agctcggaaa cctcatgagc aataacatcg tggatctcgt caatagaggg ctttttggac    10500

tccttgctgt tggccacctt gtccttgctg tttaaaccat catctaaggg cctcaaaact    10560

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10620

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10680

aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10740

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10800

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10860

tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10920

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10980

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    11040

ttcgaaatct aaactacaca tcacaccatg gtctccaacc acctgttcga cgccatgcga    11100

gctgccgctc ccggagacgc acctttcatt cgaatcgaca acgctcggac ctggacttac    11160

gatgacgcca ttgctctttc cggtcgaatc gctggagcta tggacgcact cggcattcga    11220

cccggagaca gagttgccgt gcaggtcgag aagtctgccg aggcgttgat tctctacctg    11280

gcctgtcttc gaaccggagc tgtctacctg cctctcaaca ctgcctacac cctggccgag    11340

ctcgactact tcatcggcga tgccgaaccg cgtctggtgg tcgttgctcc cgcagctcga    11400

ggtggcgtgg agacaattgc caagcgacac ggtgctatcg tcgaaaccct cgacgccgat    11460

ggacgaggct ccttgctgga ccttgctaga gatgagcctg ccgactttgt cgatgcttcg    11520

cgatctgccg acgatctggc tgctattctc tacacttccg gtacaaccgg acgatcgaag    11580

ggtgccatgc ttactcatgg caatctgctc tccaacgctc tcaccttgcg agactattgg    11640

agagttaccg cagacgatcg actcatccat gccttgccaa tctttcacac tcatggtctg    11700

ttcgttgcta cgaacgtcac actgcttgca ggagcctcga tgtttctgct ctccaagttc    11760

gatgccgacg aggtcgtttc tctcatgcca caggccacca tgcttatggg cgtgcccaca    11820

ttctacgttc gattgctgca gagtcctcga ctcgagaagg gtgctgtggc cagcatcaga    11880

ctgttcattt ctggatcagc tcccttgctt gccgaaaccc acgccgagtt tcatgctcgt    11940

actggtcacg ccattctcga gcgatacggc atgacggaaa ccaacatgaa tacttccaac    12000

ccctacgagg gcaagcgtat tgccggaacc gttggttttc ctctgcccga cgtcactgtg    12060

cgagtcaccg atcccgccac cggtctcgtt cttccacctg aagagactgg catgatcgag    12120

atcaagggac ccaacgtctt caagggctat tggcgaatgc ccgaaaagac cgctgccgag    12180

tttaccgcag acggtttctt tatctctgga gatctcggca agatcgaccg agaaggttac    12240

gttcacattg tgggacgagg caaggacctg gtcatttccg gtggctacaa catctatccc    12300

aaagaggtcg aaggcgagat cgaccagatc gagggtgtgg tcgagtctgc tgtcattggt    12360

gttcctcatc ccgatttcgg agaaggtgtc accgctgttg tcgtgtgcaa acctggtgcc    12420

gttctcgacg aaaagaccat cgtgtctgct ctgcaggacc gtcttgcccg atacaagcaa    12480

cccaagcgga ttatctttgc cgacgatctg cctcgaaaca ctatgggaaa ggttcagaag    12540

aacattcttc gacagcaata cgccgatctc tacaccagac gataagcggc cgcatgagaa    12600

gataaatata taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct    12660

cggagagaag ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa    12720

gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg    12780

gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca    12840

tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga    12900

acttgaagtg aaggaattta aatagttgga gcaagggaga aatgtagagt gtgaaagact    12960

cactatggtc cgggcttatc tcgaccaata gccaaagtct ggagtttctg agagaaaaag    13020

gcaagatacg tatgtaacaa agcgacgcat ggtacaataa taccggaggc atgtatcata    13080

gagagttagt ggttcgatga tggcactggt gcctggtatg actttatacg gctgactaca    13140

tatttgtcct cagacataca attacagtca agcacttacc cttggacatc tgtaggtacc    13200

ccccggccaa gacgatctca gcgtgtcgta tgtcggattg gcgtagctcc ctcgctcgtc    13260

aattggctcc catctacttt cttctgcttg gctacaccca gcatgtctgc tatggctcgt    13320

tttcgtgcct tatctatcct cccagtatta ccaactctaa atgacatgat gtgattgggt    13380

ctacactttc atatcagaga taaggagtag cacagttgca taaaaagccc aactctaatc    13440

agcttcttcc tttcttgtaa ttagtacaaa ggtgattagc gaaatctgga agcttagttg    13500

gccctaaaaa aatcaaaaaa agcaaaaaac gaaaaacgaa aaaccacagt tttgagaaca    13560

gggaggtaac gaaggatcgt atatatatat atatatatat atacccacgg atcccgagac    13620

cggcctttga ttcttcccta caaccaacca ttctcaccac cctaattcac aaccatggtg    13680

aaggcttctc gacaggctct gcccctcgtc atcgacggaa aggtgtacga cgtctccgct    13740

tgggtgaact tccaccctgg tggagctgaa atcattgaga actaccaggg acgagatgct    13800

actgacgcct tcatggttat gcactctcag gaagccttcg acaagctcaa gcgaatgccc    13860

aagatcaacc aggcttccga gctgcctccc caggctgccg tcaacgaagc tcaggaggat    13920

ttccgaaagc tccgagaaga gctgatcgcc actggcatgt ttgacgcctc tcccctctgg    13980

tactcgtaca agatcttgac caccctgggt cttggcgtgc ttgccttctt catgctggtc    14040

cagtaccacc tgtacttcat tggtgctctc gtgctcggta tgcactacca gcaaatggga    14100

tggctgtctc atgacatctg ccaccaccag accttcaaga accgaaactg gaataacgtc    14160

ctgggtctgg tctttggcaa cggactccag ggcttctccg tgacctggtg gaaggacaga    14220

cacaacgccc atcattctgc taccaacgtt cagggtcacg atcccgacat tgataacctg    14280

cctctgctcg cctggtccga ggacgatgtc actcgagctt ctcccatctc ccgaaagctc    14340

attcagttcc aacagtacta tttcctggtc atctgtattc tcctgcgatt catctggtgt    14400

ttccagtctg tgctgaccgt tcgatccctc aaggaccgag acaaccagtt ctaccgatct    14460

cagtacaaga aagaggccat tggactcgct ctgcactgga ctctcaagac cctgttccac    14520

ctcttcttta tgccctccat cctgacctcg atgctggtgt tctttgtttc cgagctcgtc    14580

ggtggcttcg gaattgccat cgtggtcttc atgaaccact accctctgga gaagatcggt    14640

gattccgtct gggacggaca tggcttctct gtgggtcaga tccatgagac catgaacatt    14700

cgacgaggca tcattactga ctggttcttt ggaggcctga actaccagat cgagcaccat    14760

ctctggccca ccctgcctcg acacaacctc actgccgttt cctaccaggt ggaacagctg    14820

tgccagaagc acaacctccc ctaccgaaac cctctgcccc atgaaggtct cgtcatcctg    14880

ctccgatacc tgtcccagtt cgctcgaatg gccgagaagc agcccggtgc caaggctcag    14940

taagcggccg caagtgtgga tggggaagtg agtgcccggt tctgtgtgca caattggcaa    15000

tccaagatgg atggattcaa cacagggata tagcgagcta cgtggtggtg cgaggatata    15060

gcaacggata tttatgtttg acacttgaga atgtacgata caagcactgt ccaagtacaa    15120

tactaaacat actgtacata ctcatactcg tacccgggca acggtttcac ttgagtgcag    15180

tggctagtgc tcttactcgt acagtgtgca atactgcgta tcatagtctt tgatgtatat    15240

cgtattcatt catgttagtt gc                                             15262


<210>  86
<211>  15181
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZK16-MyL8N

<400>  86
aaaccatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc tggacaccac       60

agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc agaaaacgct      120

ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg agcagggtgg      180

tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct catcaggcca      240

gattgagggt ctgtggacac atgtcatgtt agtgtacttc aatcgccccc tggatatagc      300

cccgacaata ggccgtggcc tcattttttt gccttccgca catttccatt gctcggtacc      360

cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga ccaacatctt      420

acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc ggttgccagt      480

ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca caccatggtc      540

tccaaccacc tgttcgacgc catgcgagct gccgctcccg gagacgcacc tttcattcga      600

atcgacaacg ctcggacctg gacttacgat gacgccattg ctctttccgg tcgaatcgct      660

ggagctatgg acgcactcgg cattcgaccc ggagacagag ttgccgtgca ggtcgagaag      720

tctgccgagg cgttgattct ctacctggcc tgtcttcgaa ccggagctgt ctacctgcct      780

ctcaacactg cctacaccct ggccgagctc gactacttca tcggcgatgc cgaaccgcgt      840

ctggtggtcg ttgctcccgc agctcgaggt ggcgtggaga caattgccaa gcgacacggt      900

gctatcgtcg aaaccctcga cgccgatgga cgaggctcct tgctggacct tgctagagat      960

gagcctgccg actttgtcga tgcttcgcga tctgccgacg atctggctgc tattctctac     1020

acttccggta caaccggacg atcgaagggt gccatgctta ctcatggcaa tctgctctcc     1080

aacgctctca ccttgcgaga ctattggaga gttaccgcag acgatcgact catccatgcc     1140

ttgccaatct ttcacactca tggtctgttc gttgctacga acgtcacact gcttgcagga     1200

gcctcgatgt ttctgctctc caagttcgat gccgacgagg tcgtttctct catgccacag     1260

gccaccatgc ttatgggcgt gcccacattc tacgttcgat tgctgcagag tcctcgactc     1320

gagaagggtg ctgtggccag catcagactg ttcatttctg gatcagctcc cttgcttgcc     1380

gaaacccacg ccgagtttca tgctcgtact ggtcacgcca ttctcgagcg atacggcatg     1440

acggaaacca acatgaatac ttccaacccc tacgagggca agcgtattgc cggaaccgtt     1500

ggttttcctc tgcccgacgt cactgtgcga gtcaccgatc ccgccaccgg tctcgttctt     1560

ccacctgaag agactggcat gatcgagatc aagggaccca acgtcttcaa gggctattgg     1620

cgaatgcccg aaaagaccgc tgccgagttt accgcagacg gtttctttat ctctggagat     1680

ctcggcaaga tcgaccgaga aggttacgtt cacattgtgg gacgaggcaa ggacctggtc     1740

atttccggtg gctacaacat ctatcccaaa gaggtcgaag gcgagatcga ccagatcgag     1800

ggtgtggtcg agtctgctgt cattggtgtt cctcatcccg atttcggaga aggtgtcacc     1860

gctgttgtcg tgtgcaaacc tggtgccgtt ctcgacgaaa agaccatcgt gtctgctctg     1920

caggaccgtc ttgcccgata caagcaaccc aagcggatta tctttgccga cgatctgcct     1980

cgaaacacta tgggaaaggt tcagaagaac attcttcgac agcaatacgc cgatctctac     2040

accagacgat aagcggccgc atgagaagat aaatatataa atacattgag atattaaatg     2100

cgctagatta gagagcctca tactgctcgg agagaagcca agacgagtac tcaaagggga     2160

ttacaccatc catatccaca gacacaagct ggggaaaggt tctatataca ctttccggaa     2220

taccgtagtt tccgatgtta tcaatggggg cagccaggat ttcaggcact tcggtgtctc     2280

ggggtgaaat ggcgttcttg gcctccatca agtcgtacca tgtcttcatt tgcctgtcaa     2340

agtaaaacag aagcagatga agaatgaact tgaagtgaag gaatttaaat agttggagca     2400

agggagaaat gtagagtgtg aaagactcac tatggtccgg gcttatctcg accaatagcc     2460

aaagtctgga gtttctgaga gaaaaaggca agatacgtat gtaacaaagc gacgcatggt     2520

acaataatac cggaggcatg tatcatagag agttagtggt tcgatgatgg cactggtgcc     2580

tggtatgact ttatacggct gactacatat ttgtcctcag acatacaatt acagtcaagc     2640

acttaccctt ggacatctgt aggtaccccc cggccaagac gatctcagcg tgtcgtatgt     2700

cggattggcg tagctccctc gctcgtcaat tggctcccat ctactttctt ctgcttggct     2760

acacccagca tgtctgctat ggctcgtttt cgtgccttat ctatcctccc agtattacca     2820

actctaaatg acatgatgtg attgggtcta cactttcata tcagagataa ggagtagcac     2880

agttgcataa aaagcccaac tctaatcagc ttcttccttt cttgtaatta gtacaaaggt     2940

gattagcgaa atctggaagc ttagttggcc ctaaaaaaat caaaaaaagc aaaaaacgaa     3000

aaacgaaaaa ccacagtttt gagaacaggg aggtaacgaa ggatcgtata tatatatata     3060

tatatatata cccacggatc ccgagaccgg cctttgattc ttccctacaa ccaaccattc     3120

tcaccaccct aattcacaac catggtgaag gcttctcgac aggctctgcc cctcgtcatc     3180

gacggaaagg tgtacgacgt ctccgcttgg gtgaacttcc accctggtgg agctgaaatc     3240

attgagaact accagggacg agatgctact gacgccttca tggttatgca ctctcaggaa     3300

gccttcgaca agctcaagcg aatgcccaag atcaaccagg cttccgagct gcctccccag     3360

gctgccgtca acgaagctca ggaggatttc cgaaagctcc gagaagagct gatcgccact     3420

ggcatgtttg acgcctctcc cctctggtac tcgtacaaga tcttgaccac cctgggtctt     3480

ggcgtgcttg ccttcttcat gctggtccag taccacctgt acttcattgg tgctctcgtg     3540

ctcggtatgc actaccagca aatgggatgg ctgtctcatg acatctgcca ccaccagacc     3600

ttcaagaacc gaaactggaa taacgtcctg ggtctggtct ttggcaacgg actccagggc     3660

ttctccgtga cctggtggaa ggacagacac aacgcccatc attctgctac caacgttcag     3720

ggtcacgatc ccgacattga taacctgcct ctgctcgcct ggtccgagga cgatgtcact     3780

cgagcttctc ccatctcccg aaagctcatt cagttccaac agtactattt cctggtcatc     3840

tgtattctcc tgcgattcat ctggtgtttc cagtctgtgc tgaccgttcg atccctcaag     3900

gaccgagaca accagttcta ccgatctcag tacaagaaag aggccattgg actcgctctg     3960

cactggactc tcaagaccct gttccacctc ttctttatgc cctccatcct gacctcgatg     4020

ctggtgttct ttgtttccga gctcgtcggt ggcttcggaa ttgccatcgt ggtcttcatg     4080

aaccactacc ctctggagaa gatcggtgat tccgtctggg acggacatgg cttctctgtg     4140

ggtcagatcc atgagaccat gaacattcga cgaggcatca ttactgactg gttctttgga     4200

ggcctgaact accagatcga gcaccatctc tggcccaccc tgcctcgaca caacctcact     4260

gccgtttcct accaggtgga acagctgtgc cagaagcaca acctccccta ccgaaaccct     4320

ctgccccatg aaggtctcgt catcctgctc cgatacctgt cccagttcgc tcgaatggcc     4380

gagaagcagc ccggtgccaa ggctcagtaa gcggccgcaa gtgtggatgg ggaagtgagt     4440

gcccggttct gtgtgcacaa ttggcaatcc aagatggatg gattcaacac agggatatag     4500

cgagctacgt ggtggtgcga ggatatagca acggatattt atgtttgaca cttgagaatg     4560

tacgatacaa gcactgtcca agtacaatac taaacatact gtacatactc atactcgtac     4620

ccgggcaacg gtttcacttg agtgcagtgg ctagtgctct tactcgtaca gtgtgcaata     4680

ctgcgtatca tagtctttga tgtatatcgt attcattcat gttagttgcg tacgcattct     4740

tgacttgctc cccagttgcc ctttttggtc ataactcagc cccgactctc tccccaaaga     4800

ggagatggac ttacaataga tacgagacaa cagatatttt cggccacctg gtgcaattgg     4860

tctgttttat tacccccaat aatatccaca gcgtccgaat cagctcgaca agctctttct     4920

cagcatactg atcttacccc atacccccca gacagcggtg ccatatccat acccgcacta     4980

cctgagacag taaactcagg gcaaggagcg gtcttggtcc atcgcgacgc tgtaagcttt     5040

gcctttgcgt tttgtgatcc accacacggc agtcaaggtc tcggttgaaa gatgggtctg     5100

gcaatgtgga ttgctgaatc attgacatat aaatacgagt gatcctgcca tttcccaatc     5160

tttagaaatc atcttttctt cacacataca aaaaacacac atcacaatgc agttctccgc     5220

cgccgccgtc cttgccctcg ccgccttcgt ctctgcccag gccgcctccg ctgcttctga     5280

agctggtggt gccgttgtct ccggtgcctc cggtgccgct gccgccgctt cttctgctgt     5340

cattggctcc gagtctcagg ccgcttcttc tgctgcctct tctgctgctg agtctgcttc     5400

ttccgctgcc tctgagtccg cctcctccgc ttccgcctcc gagtccaagg cctctgagtc     5460

cgcttccaag tccgcctcca aggcttctga gtctgcttcc aaggccgagt cttctgctgc     5520

caaggcttcc tccgctgctg gttctgcttc ttccgccgct gcctctgcca ccaagtctgg     5580

tgatgcctcc ggctcttcca agccttccgg ctctgcctcc tctaccggtg acaagtccgg     5640

tgctggtatt gttgcccccg ctgttggtgc catcgttgtc ggtgctcttg ctcagctcat     5700

ctaagcggtc tttgtacaac tacccactta atatttatat tatgatatag actgcaatac     5760

tattgcatgt gtaatgcttc gtttgaaagt cgtagtccgg ccattcatgg cagtgtatgg     5820

cagtattgca accaattggc ttaataacat atacaaagtg gcaattctgt gttcgaacca     5880

tcacacaacc caacatattt gacattgcta caagtattcg acttgttctt cggccgcacg     5940

acatgcattg ctagcagcac acttgcagaa gcttggccaa tggttaggtt gacctttaac     6000

cagtaaaacg caatataggt tgccaattaa caaacaacac agcccagaac tggtgtaaag     6060

ggcttgggtt ttgtgcaatc aactcattga ctcacctccc ccaatgctcc atgaacccat     6120

gaatccatag tacctccatc ccaattcaga gaggctcaga aacacgcaat tggcaaaagt     6180

aaacaaaggt ctcgaaacct taaggggtgc agtgttgcaa agttaaaatt acactgttgg     6240

atggtgttcc actcaaactg tggatggatc agacggttgc actaaaccaa gattcaatat     6300

gctctacaag tagcgtgcca tgaggtgtat aatatcacac atgatcagag ttaaggcctg     6360

gaaaaaaagt gtccgcggct atgtaacact gctgaataca gtgtgtgccg tttccacgga     6420

ctggaatgaa tgcactcaac acgttggtag ctaacttccc caactattga cttcacttta     6480

ctgtagctac tctaagtacg ataatgacat catgaatcac caactttatc ttccccaccg     6540

aaagtaagag atgaaagaga ggctaacagc ttttgtaaga aaatcacaaa atctacttac     6600

cgtattacaa aagtacaagt atcagtaacc aggcgcgcca gctgcattaa tgaatcggcc     6660

aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact     6720

cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac     6780

ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa     6840

aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg     6900

acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa     6960

gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc     7020

ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac     7080

gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac     7140

cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg     7200

taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt     7260

atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa     7320

cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct     7380

cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga     7440

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg     7500

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct     7560

tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt     7620

aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc     7680

tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg     7740

gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag     7800

atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt     7860

tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag     7920

ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt     7980

ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca     8040

tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg     8100

ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat     8160

ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta     8220

tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca     8280

gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct     8340

taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat     8400

cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa     8460

agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt     8520

gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa     8580

ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa     8640

ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt     8700

tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     8760

tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag     8820

tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     8880

tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga     8940

ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     9000

gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     9060

cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc     9120

cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt     9180

gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag     9240

ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta     9300

atacgactca ctatagggcg aattgggccc gacgtcgcat gctatgtaca ctgtgtgagt     9360

gtagagggca atggtggtga caactgagct actatacaca cacagcttgt actggtataa     9420

gttgtgagct acagtacaag taatactaca aaattcagta tgtacaagta tgccggtacg     9480

gcagtagcat gtatcaaaga gagacagaaa gaaggctgga aagcgaaggc cagctgagcg     9540

tgggaaccgc agtacgtgga agtgccaaac ctgcgacaga atgacatggt cttgcacttt     9600

ttaccggtac cggtacagta cgataccggt acgcacttgc tacagtagat catagaccac     9660

cagaataacc ctgcattagc gccacgttgc caagccctca actttctttg cagctacacc     9720

ttttacacct tttaggctgc caggaagagc ataaatgaat tgtacttggg ggcacagaat     9780

ttgggttgaa gtgttcctgg gagagaatgt gcttggctgt atagttgggt ggctctcctt     9840

catagtagat gtgaggttac agcacttgta gattgtgtac gcgttctgtt taaggtggta     9900

ccagtattat gggttgacgt gaaagaaagg gaggaggcag gggtacagca agagtgtggg     9960

cgcacactca cacatgaagc aagacctgtt tcgtcatttc tatttgttta acaaggacga    10020

cataatatac ttaggacagc atgggccgac cggcaggaat aatctgcaaa cacaatcgta    10080

ttgtggcgtc ccctctcgat cacgatagtt gctaagttta gcagtctgag agtttggctg    10140

tgacgcaaga ccgagagaga gacagagagt tgtcccagtt tgagtactcg taccgtacta    10200

cagagtaaat cgaaatcgag cgctcacttt ctgcactttg tgaaggcgca gcattcaaca    10260

tttgtgtcca gcccctttcc gtttgaagcg acttgaccac gaaatgttgg aataaagctg    10320

tgttacatac gagacctaca agtagagtgt cggatatgcc aactgaacag gttagatgaa    10380

agttactcaa caacaggaga gcggacacca acgtgcgaaa atgtacggat gaacccattg    10440

tctgtccgca tagcaatctg ttttcgccag cagtgcttgt gtcgttggat tgctgccatt    10500

gtactatttg tactttgttc cattaacact cgcatcttgc tcaccgatta gcagcactcc    10560

tgttgcctca ctgggcttgt ctgtttcagg gtggaatgca tgagcaatgc gatcgtcaag    10620

gggaggttga aagcgcgtat ggaataactg tggttctata gctgagggga ggatatcgag    10680

gacagccacg gttcagtggg ggatttcggg agctactcag taacatgtac tcttgtccga    10740

acgccctgct gatggcaaat atgacaaaac gcaacaatgt ggagcaacat ttccatttcg    10800

agagatatta catgggttgc attggggcct gggcatcccg tgcaataacc gagaccatgt    10860

gaaggtgcac gatcgacact tatccagcca gtcatacgtg gtgtcgcaag ctaagcaaca    10920

atgggattca cgaatctatg tgggggaacg tgcacagggg aaatgaaaca aaacggagga    10980

gggggtcttt gaaaatgtag agaaatataa caaaaaatca caaaaacgca ccaaaaatat    11040

gccaaaatcg tcaacagtgg aaatcacgcg cccctggctc gacttcccac tgacataccc    11100

cccctcaacc aatatcatat ccaccctagc caatcgctta attaagttgc gacacatgtc    11160

ttgatagtat cttgaattct ctctcttgag cttttccata acaagttctt ctgcctccag    11220

gaagtccatg ggtggtttga tcatggtttt ggtgtagtgg tagtgcagtg gtggtattgt    11280

gactggggat gtagttgaga ataagtcata cacaagtcag ctttcttcga gcctcatata    11340

agtataagta gttcaacgta ttagcactgt acccagcatc tccgtatcga gaaacacaac    11400

aacatgcccc attggacaga tcatgcggat acacaggttg tgcagtatca tacatactcg    11460

atcagacagg tcgtctgacc atcatacaag ctgaacaagc gctccatact tgcacgctct    11520

ctatatacac agttaaatta catatccata gtctaacctc taacagttaa tcttctggta    11580

agcctcccag ccagccttct ggtatcgctt ggcctcctca ataggatctc ggttctggcc    11640

gtacagacct cggccgacaa ttatgatatc cgttccggta gacatgacat cctcaacagt    11700

tcggtactgc tgtccgagag cgtctccctt gtcgtcaaga cccaccccgg gggtcagaat    11760

aagccagtcc tcagagtcgc ccttaggtcg gttctgggca atgaagccaa ccacaaactc    11820

ggggtcggat cgggcaagct caatggtctg cttggagtac tcgccagtgg ccagagagcc    11880

cttgcaagac agctcggcca gcatgagcag acctctggcc agcttctcgt tgggagaggg    11940

gactaggaac tccttgtact gggagttctc gtagtcagag acgtcctcct tcttctgttc    12000

agagacagtt tcctcggcac cagctcgcag gccagcaatg attccggttc cgggtacacc    12060

gtgggcgttg gtgatatcgg accactcggc gattcggtga caccggtact ggtgcttgac    12120

agtgttgcca atatctgcga actttctgtc ctcgaacagg aagaaaccgt gcttaagagc    12180

aagttccttg agggggagca cagtgccggc gtaggtgaag tcgtcaatga tgtcgatatg    12240

ggttttgatc atgcacacat aaggtccgac cttatcggca agctcaatga gctccttggt    12300

ggtggtaaca tccagagaag cacacaggtt ggttttcttg gctgccacga gcttgagcac    12360

tcgagcggca aaggcggact tgtggacgtt agctcgagct tcgtaggagg gcattttggt    12420

ggtgaagagg agactgaaat aaatttagtc tgcagaactt tttatcggaa ccttatctgg    12480

ggcagtgaag tatatgttat ggtaatagtt acgagttagt tgaacttata gatagactgg    12540

actatacggc tatcggtcca aattagaaag aacgtcaatg gctctctggg cgtcgccttt    12600

gccgacaaaa atgtgatcat gatgaaagcc agcaatgacg ttgcagctga tattgttgtc    12660

ggccaaccgc gccgaaaacg cagctgtcag acccacagcc tccaacgaag aatgtatcgt    12720

caaagtgatc caagcacact catagttgga gtcgtactcc aaaggcggca atgacgagtc    12780

agacagatac tcgtcgacct tttccttggg aaccaccacc gtcagccctt ctgactcacg    12840

tattgtagcc accgacacag gcaacagtcc gtggatagca gaatatgtct tgtcggtcca    12900

tttctcacca actttaggcg tcaagtgaat gttgcagaag aagtatgtgc cttcattgag    12960

aatcggtgtt gctgatttca ataaagtctt gagatcagtt tggccagtca tgttgtgggg    13020

ggtaattgga ttgagttatc gcctacagtc tgtacaggta tactcgctgc ccactttata    13080

ctttttgatt ccgctgcact tgaagcaatg tcgtttacca aaagtgagaa tgctccacag    13140

aacacacccc agggtatggt tgagcaaaaa ataaacactc cgatacgggg aatcgaaccc    13200

cggtctccac ggttctcaag aagtattctt gatgagagcg tatcgatagt tggagcaagg    13260

gagaaatgta gagtgtgaaa gactcactat ggtccgggct tatctcgacc aatagccaaa    13320

gtctggagtt tctgagagaa aaaggcaaga tacgtatgta acaaagcgac gcatggtaca    13380

ataataccgg aggcatgtat catagagagt tagtggttcg atgatggcac tggtgcctgg    13440

tatgacttta tacggctgac tacatatttg tcctcagaca tacaattaca gtcaagcact    13500

tacccttgga catctgtagg taccccccgg ccaagacgat ctcagcgtgt cgtatgtcgg    13560

attggcgtag ctccctcgct cgtcaattgg ctcccatcta ctttcttctg cttggctaca    13620

cccagcatgt ctgctatggc tcgttttcgt gccttatcta tcctcccagt attaccaact    13680

ctaaatgaca tgatgtgatt gggtctacac tttcatatca gagataagga gtagcacagt    13740

tgcataaaaa gcccaactct aatcagcttc ttcctttctt gtaattagta caaaggtgat    13800

tagcgaaatc tggaagctta gttggcccta aaaaaatcaa aaaaagcaaa aaacgaaaaa    13860

cgaaaaacca cagttttgag aacagggagg taacgaagga tcgtatatat atatatatat    13920

atatataccc acggatcccg agaccggcct ttgattcttc cctacaacca accattctca    13980

ccaccctaat tcacaaccat gtccgttgca tccaagctcg tcttctacgt ccgcgccgcc    14040

atcgccgtgg tcatctttgc cgcctgtgcc acctacggcg tgctggcgtc caccattctc    14100

accgccatcg gcaagcaggg cctggcccaa tggaccgttg ccagagcctt ctactactcg    14160

gtgcgcatct tcctgggtat cagcatcaag ctgcgtagcc ggcaggtgac cggaaccgcc    14220

ggtctggatg cctccaagat ccaggtcgcc aacaccacca agcccattga cgacatcacc    14280

aaacacctgc cccgaccatg cattctgatt tccaaccacc agaacgaaat ggacattctg    14340

gtgctcggtc gcatcttccc ccagtactgc tccgtcaccg ccaaaaaggc cctcaagtgg    14400

taccctctgc tgggccagtt catggcgctg tccggcacca tcttcctgga ccgaaaggac    14460

cgaaccaagt ccgtgcagac cctcggcggc gccgtcaaga ccatccagag cggcaacgga    14520

ggcaagggcc agagcgtctt catgttcccc gagggaaccc gatcctactc caaggacgtc    14580

ggcatcatgc ccttcaagaa gggctgtttc cacctggcgg tccagtcggg cgctcccatt    14640

gtccccgtgg tggtccagaa cacctcccga atgttttctt tcggccgagg caagctggac    14700

gccggagaga tccttgtcga cgtcctgagc cccattgaga ccaagggtct ggacgccagc    14760

aacgtcgacg ctctcatggc caccacttat aaggccatgt gcgagactgc cgaccagatt    14820

ggctacgctg gccagaagac tcagtaggcg gccgcatgag aagataaata tataaataca    14880

ttgagatatt aaatgcgcta gattagagag cctcatactg ctcggagaga agccaagacg    14940

agtactcaaa ggggattaca ccatccatat ccacagacac aagctgggga aaggttctat    15000

atacactttc cggaataccg tagtttccga tgttatcaat gggggcagcc aggatttcag    15060

gcacttcggt gtctcggggt gaaatggcgt tcttggcctc catcaagtcg taccatgtct    15120

tcatttgcct gtcaaagtaa aacagaagca gatgaagaat gaacttgaag tgaaggaatt    15180

t                                                                    15181


<210>  87
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant EgD8S-23 delta-8 desaturase (also "EgD8M")


<220>
<221>  CDS
<222>  (2)..(1270)

<300>
<302>  MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS
        AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
<310>  US 7709239
<311>  2006-12-07
<312>  2010-05-04
<313>  (1)..(1272)

<400>  87
c atg gtg aag gct tct cga cag gct ctg ccc ctc gtc atc gac gga aag       49
  Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys         
  1               5                   10                  15              

gtg tac gac gtc tcc gct tgg gtg aac ttc cac cct ggt gga gct gaa         97
Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu           
            20                  25                  30                    

atc att gag aac tac cag gga cga gat gct act gac gcc ttc atg gtt        145
Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val           
        35                  40                  45                        

atg cac tct cag gaa gcc ttc gac aag ctc aag cga atg ccc aag atc        193
Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile           
    50                  55                  60                            

aac cag gct tcc gag ctg cct ccc cag gct gcc gtc aac gaa gct cag        241
Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln           
65                  70                  75                  80            

gag gat ttc cga aag ctc cga gaa gag ctg atc gcc act ggc atg ttt        289
Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe           
                85                  90                  95                

gac gcc tct ccc ctc tgg tac tcg tac aag atc ttg acc acc ctg ggt        337
Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly           
            100                 105                 110                   

ctt ggc gtg ctt gcc ttc ttc atg ctg gtc cag tac cac ctg tac ttc        385
Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe           
        115                 120                 125                       

att ggt gct ctc gtg ctc ggt atg cac tac cag caa atg gga tgg ctg        433
Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu           
    130                 135                 140                           

tct cat gac atc tgc cac cac cag acc ttc aag aac cga aac tgg aat        481
Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn           
145                 150                 155                 160           

aac gtc ctg ggt ctg gtc ttt ggc aac gga ctc cag ggc ttc tcc gtg        529
Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val           
                165                 170                 175               

acc tgg tgg aag gac aga cac aac gcc cat cat tct gct acc aac gtt        577
Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val           
            180                 185                 190                   

cag ggt cac gat ccc gac att gat aac ctg cct ctg ctc gcc tgg tcc        625
Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser           
        195                 200                 205                       

gag gac gat gtc act cga gct tct ccc atc tcc cga aag ctc att cag        673
Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln           
    210                 215                 220                           

ttc caa cag tac tat ttc ctg gtc atc tgt att ctc ctg cga ttc atc        721
Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile           
225                 230                 235                 240           

tgg tgt ttc cag tct gtg ctg acc gtt cga tcc ctc aag gac cga gac        769
Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp           
                245                 250                 255               

aac cag ttc tac cga tct cag tac aag aaa gag gcc att gga ctc gct        817
Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala           
            260                 265                 270                   

ctg cac tgg act ctc aag acc ctg ttc cac ctc ttc ttt atg ccc tcc        865
Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser           
        275                 280                 285                       

atc ctg acc tcg atg ctg gtg ttc ttt gtt tcc gag ctc gtc ggt ggc        913
Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly           
    290                 295                 300                           

ttc gga att gcc atc gtg gtc ttc atg aac cac tac cct ctg gag aag        961
Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys           
305                 310                 315                 320           

atc ggt gat tcc gtc tgg gac gga cat ggc ttc tct gtg ggt cag atc       1009
Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile           
                325                 330                 335               

cat gag acc atg aac att cga cga ggc atc att act gac tgg ttc ttt       1057
His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe           
            340                 345                 350                   

gga ggc ctg aac tac cag atc gag cac cat ctc tgg ccc acc ctg cct       1105
Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro           
        355                 360                 365                       

cga cac aac ctc act gcc gtt tcc tac cag gtg gaa cag ctg tgc cag       1153
Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln           
    370                 375                 380                           

aag cac aac ctc ccc tac cga aac cct ctg ccc cat gaa ggt ctc gtc       1201
Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val           
385                 390                 395                 400           

atc ctg ctc cga tac ctg tcc cag ttc gct cga atg gcc gag aag cag       1249
Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln           
                405                 410                 415               

ccc ggt gcc aag gct cag taa gc                                        1272
Pro Gly Ala Lys Ala Gln                                                   
            420                                                           


<210>  88
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  88

Met Val Lys Ala Ser Arg Gln Ala Leu Pro Leu Val Ile Asp Gly Lys 
1               5                   10                  15      


Val Tyr Asp Val Ser Ala Trp Val Asn Phe His Pro Gly Gly Ala Glu 
            20                  25                  30          


Ile Ile Glu Asn Tyr Gln Gly Arg Asp Ala Thr Asp Ala Phe Met Val 
        35                  40                  45              


Met His Ser Gln Glu Ala Phe Asp Lys Leu Lys Arg Met Pro Lys Ile 
    50                  55                  60                  


Asn Gln Ala Ser Glu Leu Pro Pro Gln Ala Ala Val Asn Glu Ala Gln 
65                  70                  75                  80  


Glu Asp Phe Arg Lys Leu Arg Glu Glu Leu Ile Ala Thr Gly Met Phe 
                85                  90                  95      


Asp Ala Ser Pro Leu Trp Tyr Ser Tyr Lys Ile Leu Thr Thr Leu Gly 
            100                 105                 110         


Leu Gly Val Leu Ala Phe Phe Met Leu Val Gln Tyr His Leu Tyr Phe 
        115                 120                 125             


Ile Gly Ala Leu Val Leu Gly Met His Tyr Gln Gln Met Gly Trp Leu 
    130                 135                 140                 


Ser His Asp Ile Cys His His Gln Thr Phe Lys Asn Arg Asn Trp Asn 
145                 150                 155                 160 


Asn Val Leu Gly Leu Val Phe Gly Asn Gly Leu Gln Gly Phe Ser Val 
                165                 170                 175     


Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn Val 
            180                 185                 190         


Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp Ser 
        195                 200                 205             


Glu Asp Asp Val Thr Arg Ala Ser Pro Ile Ser Arg Lys Leu Ile Gln 
    210                 215                 220                 


Phe Gln Gln Tyr Tyr Phe Leu Val Ile Cys Ile Leu Leu Arg Phe Ile 
225                 230                 235                 240 


Trp Cys Phe Gln Ser Val Leu Thr Val Arg Ser Leu Lys Asp Arg Asp 
                245                 250                 255     


Asn Gln Phe Tyr Arg Ser Gln Tyr Lys Lys Glu Ala Ile Gly Leu Ala 
            260                 265                 270         


Leu His Trp Thr Leu Lys Thr Leu Phe His Leu Phe Phe Met Pro Ser 
        275                 280                 285             


Ile Leu Thr Ser Met Leu Val Phe Phe Val Ser Glu Leu Val Gly Gly 
    290                 295                 300                 


Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu Lys 
305                 310                 315                 320 


Ile Gly Asp Ser Val Trp Asp Gly His Gly Phe Ser Val Gly Gln Ile 
                325                 330                 335     


His Glu Thr Met Asn Ile Arg Arg Gly Ile Ile Thr Asp Trp Phe Phe 
            340                 345                 350         


Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu Pro 
        355                 360                 365             


Arg His Asn Leu Thr Ala Val Ser Tyr Gln Val Glu Gln Leu Cys Gln 
    370                 375                 380                 


Lys His Asn Leu Pro Tyr Arg Asn Pro Leu Pro His Glu Gly Leu Val 
385                 390                 395                 400 


Ile Leu Leu Arg Tyr Leu Ser Gln Phe Ala Arg Met Ala Glu Lys Gln 
                405                 410                 415     


Pro Gly Ala Lys Ala Gln 
            420         


<210>  89
<211>  15105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZK16-ML3

<400>  89
gtacgcattc ttgacttgct ccccagttgc cctttttggt cataactcag ccccgactct       60

ctccccaaag aggagatgga cttacaatag atacgagaca acagatattt tcggccacct      120

ggtgcaattg gtctgtttta ttacccccaa taatatccac agcgtccgaa tcagctcgac      180

aagctctttc tcagcatact gatcttaccc catacccccc agacagcggt gccatatcca      240

tacccgcact acctgagaca gtaaactcag ggcaaggagc ggtcttggtc catcgcgacg      300

ctgtaagctt tgcctttgcg ttttgtgatc caccacacgg cagtcaaggt ctcggttgaa      360

agatgggtct ggcaatgtgg attgctgaat cattgacata taaatacgag tgatcctgcc      420

atttcccaat ctttagaaat catcttttct tcacacatac aaaaaacaca catcacaatg      480

cagttctccg ccgccgccgt ccttgccctc gccgccttcg tctctgccca ggccgcctcc      540

gctgcttctg aagctggtgg tgccgttgtc tccggtgcct ccggtgccgc tgccgccgct      600

tcttctgctg tcattggctc cgagtctcag gccgcttctt ctgctgcctc ttctgctgct      660

gagtctgctt cttccgctgc ctctgagtcc gcctcctccg cttccgcctc cgagtccaag      720

gcctctgagt ccgcttccaa gtccgcctcc aaggcttctg agtctgcttc caaggccgag      780

tcttctgctg ccaaggcttc ctccgctgct ggttctgctt cttccgccgc tgcctctgcc      840

accaagtctg gtgatgcctc cggctcttcc aagccttccg gctctgcctc ctctaccggt      900

gacaagtccg gtgctggtat tgttgccccc gctgttggtg ccatcgttgt cggtgctctt      960

gctcagctca tctaagcggt ctttgtacaa ctacccactt aatatttata ttatgatata     1020

gactgcaata ctattgcatg tgtaatgctt cgtttgaaag tcgtagtccg gccattcatg     1080

gcagtgtatg gcagtattgc aaccaattgg cttaataaca tatacaaagt ggcaattctg     1140

tgttcgaacc atcacacaac ccaacatatt tgacattgct acaagtattc gacttgttct     1200

tcggccgcac gacatgcatt gctagcagca cacttgcaga agcttggcca atggttaggt     1260

tgacctttaa ccagtaaaac gcaatatagg ttgccaatta acaaacaaca cagcccagaa     1320

ctggtgtaaa gggcttgggt tttgtgcaat caactcattg actcacctcc cccaatgctc     1380

catgaaccca tgaatccata gtacctccat cccaattcag agaggctcag aaacacgcaa     1440

ttggcaaaag taaacaaagg tctcgaaacc ttaaggggtg cagtgttgca aagttaaaat     1500

tacactgttg gatggtgttc cactcaaact gtggatggat cagacggttg cactaaacca     1560

agattcaata tgctctacaa gtagcgtgcc atgaggtgta taatatcaca catgatcaga     1620

gttaaggcct ggaaaaaaag tgtccgcggc tatgtaacac tgctgaatac agtgtgtgcc     1680

gtttccacgg actggaatga atgcactcaa cacgttggta gctaacttcc ccaactattg     1740

acttcacttt actgtagcta ctctaagtac gataatgaca tcatgaatca ccaactttat     1800

cttccccacc gaaagtaaga gatgaaagag aggctaacag cttttgtaag aaaatcacaa     1860

aatctactta ccgtattaca aaagtacaag tatcagtaac caggcgcgcc agctgcatta     1920

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     1980

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     2040

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     2100

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     2160

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     2220

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     2280

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     2340

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     2400

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     2460

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     2520

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     2580

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     2640

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     2700

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     2760

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     2820

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     2880

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     2940

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     3000

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     3060

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     3120

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     3180

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc     3240

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     3300

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     3360

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     3420

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     3480

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc     3540

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     3600

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     3660

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     3720

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     3780

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     3840

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     3900

tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag     3960

cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca     4020

ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag     4080

tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg     4140

gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt     4200

tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag     4260

agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc     4320

gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc     4380

gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa     4440

gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca     4500

aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc     4560

agtgaattgt aatacgactc actatagggc gaattgggcc cgacgtcgca tgctatgtac     4620

actgtgtgag tgtagagggc aatggtggtg acaactgagc tactatacac acacagcttg     4680

tactggtata agttgtgagc tacagtacaa gtaatactac aaaattcagt atgtacaagt     4740

atgccggtac ggcagtagca tgtatcaaag agagacagaa agaaggctgg aaagcgaagg     4800

ccagctgagc gtgggaaccg cagtacgtgg aagtgccaaa cctgcgacag aatgacatgg     4860

tcttgcactt tttaccggta ccggtacagt acgataccgg tacgcacttg ctacagtaga     4920

tcatagacca ccagaataac cctgcattag cgccacgttg ccaagccctc aactttcttt     4980

gcagctacac cttttacacc ttttaggctg ccaggaagag cataaatgaa ttgtacttgg     5040

gggcacagaa tttgggttga agtgttcctg ggagagaatg tgcttggctg tatagttggg     5100

tggctctcct tcatagtaga tgtgaggtta cagcacttgt agattgtgta cgcgttctgt     5160

ttaaggtggt accagtatta tgggttgacg tgaaagaaag ggaggaggca ggggtacagc     5220

aagagtgtgg gcgcacactc acacatgaag caagacctgt ttcgtcattt ctatttgttt     5280

aacaaggacg acataatata cttaggacag catgggccga ccggcaggaa taatctgcaa     5340

acacaatcgt attgtggcgt cccctctcga tcacgatagt tgctaagttt agcagtctga     5400

gagtttggct gtgacgcaag accgagagag agacagagag ttgtcccagt ttgagtactc     5460

gtaccgtact acagagtaaa tcgaaatcga gcgctcactt tctgcacttt gtgaaggcgc     5520

agcattcaac atttgtgtcc agcccctttc cgtttgaagc gacttgacca cgaaatgttg     5580

gaataaagct gtgttacata cgagacctac aagtagagtg tcggatatgc caactgaaca     5640

ggttagatga aagttactca acaacaggag agcggacacc aacgtgcgaa aatgtacgga     5700

tgaacccatt gtctgtccgc atagcaatct gttttcgcca gcagtgcttg tgtcgttgga     5760

ttgctgccat tgtactattt gtactttgtt ccattaacac tcgcatcttg ctcaccgatt     5820

agcagcactc ctgttgcctc actgggcttg tctgtttcag ggtggaatgc atgagcaatg     5880

cgatcgtcaa ggggaggttg aaagcgcgta tggaataact gtggttctat agctgagggg     5940

aggatatcga ggacagccac ggttcagtgg gggatttcgg gagctactca gtaacatgta     6000

ctcttgtccg aacgccctgc tgatggcaaa tatgacaaaa cgcaacaatg tggagcaaca     6060

tttccatttc gagagatatt acatgggttg cattggggcc tgggcatccc gtgcaataac     6120

cgagaccatg tgaaggtgca cgatcgacac ttatccagcc agtcatacgt ggtgtcgcaa     6180

gctaagcaac aatgggattc acgaatctat gtgggggaac gtgcacaggg gaaatgaaac     6240

aaaacggagg agggggtctt tgaaaatgta gagaaatata acaaaaaatc acaaaaacgc     6300

accaaaaata tgccaaaatc gtcaacagtg gaaatcacgc gcccctggct cgacttccca     6360

ctgacatacc ccccctcaac caatatcata tccaccctag ccaatcgctt aattaagttg     6420

cgacacatgt cttgatagta tcttgaattc tctctcttga gcttttccat aacaagttct     6480

tctgcctcca ggaagtccat gggtggtttg atcatggttt tggtgtagtg gtagtgcagt     6540

ggtggtattg tgactgggga tgtagttgag aataagtcat acacaagtca gctttcttcg     6600

agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     6660

agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     6720

atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     6780

ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     6840

atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     6900

cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     6960

tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     7020

ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     7080

accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     7140

gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     7200

ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     7260

ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     7320

ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     7380

tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     7440

tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     7500

atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     7560

agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     7620

agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     7680

ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     7740

accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     7800

agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     7860

gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg     7920

atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa     7980

gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc     8040

aatgacgagt cagacagata ctcgtcgacc ttttccttgg gaaccaccac cgtcagccct     8100

tctgactcac gtattgtagc caccgacaca ggcaacagtc cgtggatagc agaatatgtc     8160

ttgtcggtcc atttctcacc aactttaggc gtcaagtgaa tgttgcagaa gaagtatgtg     8220

ccttcattga gaatcggtgt tgctgatttc aataaagtct tgagatcagt ttggccagtc     8280

atgttgtggg gggtaattgg attgagttat cgcctacagt ctgtacaggt atactcgctg     8340

cccactttat actttttgat tccgctgcac ttgaagcaat gtcgtttacc aaaagtgaga     8400

atgctccaca gaacacaccc cagggtatgg ttgagcaaaa aataaacact ccgatacggg     8460

gaatcgaacc ccggtctcca cggttctcaa gaagtattct tgatgagagc gtatcgatag     8520

ttggagcaag ggagaaatgt agagtgtgaa agactcacta tggtccgggc ttatctcgac     8580

caatagccaa agtctggagt ttctgagaga aaaaggcaag atacgtatgt aacaaagcga     8640

cgcatggtac aataataccg gaggcatgta tcatagagag ttagtggttc gatgatggca     8700

ctggtgcctg gtatgacttt atacggctga ctacatattt gtcctcagac atacaattac     8760

agtcaagcac ttacccttgg acatctgtag gtaccccccg gccaagacga tctcagcgtg     8820

tcgtatgtcg gattggcgta gctccctcgc tcgtcaattg gctcccatct actttcttct     8880

gcttggctac acccagcatg tctgctatgg ctcgttttcg tgccttatct atcctcccag     8940

tattaccaac tctaaatgac atgatgtgat tgggtctaca ctttcatatc agagataagg     9000

agtagcacag ttgcataaaa agcccaactc taatcagctt cttcctttct tgtaattagt     9060

acaaaggtga ttagcgaaat ctggaagctt agttggccct aaaaaaatca aaaaaagcaa     9120

aaaacgaaaa acgaaaaacc acagttttga gaacagggag gtaacgaagg atcgtatata     9180

tatatatata tatatatacc cacggatccc gagaccggcc tttgattctt ccctacaacc     9240

aaccattctc accaccctaa ttcacaacca tgtctattgg ttcgtccaac cccgtgctct     9300

tggctgcgat tcccttcgtc tacctgtttg tcctcccacg agtcctggct ttcctgcctc     9360

agaaggctca gttcctggcc aaatgtattg tggtcctgat tgccacgctt atcatgtccg     9420

ttgcaggctg cttcatctcg atcgtgtgcg ctcttctgga caagagatac gtcatcaatt     9480

acgttgtgtc gcgattgttc tccttccttg ccgctcgacc gtgtggtgtg acctataaga     9540

ttgttggtga ggaacacctc gataagtacc ctgctatcgt ggtctgtaac catcaatcct     9600

ctatggatat gatggttttg ggacgagttt ttccaaagca ctgcgttgtc atggcgaaga     9660

aggaactcct gtactttccc tttttgggaa tgtttatgaa actgagcaac gctatcttca     9720

tcgaccggaa gaaccacaag aaagccatcg agtctaccac ccaagccgtg gcggacatga     9780

agaagcacaa ctctggaatc tggattttcc cagagggcac ccggtctaga ctggacaagg     9840

cagacctgct gcccttcaag aaaggtgcct ttcatcttgc aattcaggcc cagctcccta     9900

ttctccccat tatctcgcag ggctattccc atatctacga ctcttcgaag cggtacttcc     9960

ccggtggaga gctcgagatc agagtcctgg agcccattcc tacaactggc ctcactactg    10020

atgatgtgaa cgacctgatg gacaagacac gaaaccttat gctcaagcac ttgaaggaga    10080

tggattccca gtattcgtcg agcactgctg aaaatggatc cacgcacatc gacgccgata    10140

ttgccaagtc tacagccacc agcattggca acactgacga cgcaattaca aaacgtcgta    10200

cccctaagga ataagcggcc gcattgatga ttggaaacac acacatgggt tatatctagg    10260

tgagagttag ttggacagtt atatattaaa tcagctatgc caacggtaac ttcattcatg    10320

tcaacgagga accagtgact gcaagtaata tagaatttga ccaccttgcc attctcttgc    10380

actcctttac tatatctcat ttatttctta tatacaaatc acttcttctt cccagcatcg    10440

agctcggaaa cctcatgagc aataacatcg tggatctcgt caatagaggg ctttttggac    10500

tccttgctgt tggccacctt gtccttgctg tttaaaccat catctaaggg cctcaaaact    10560

acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10620

caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10680

aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10740

cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10800

gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10860

tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10920

accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10980

tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    11040

ttcgaaatct aaactacaca tcacaccatg gtctccaacc acctgttcga cgccatgcga    11100

gctgccgctc ccggagacgc acctttcatt cgaatcgaca acgctcggac ctggacttac    11160

gatgacgcca ttgctctttc cggtcgaatc gctggagcta tggacgcact cggcattcga    11220

cccggagaca gagttgccgt gcaggtcgag aagtctgccg aggcgttgat tctctacctg    11280

gcctgtcttc gaaccggagc tgtctacctg cctctcaaca ctgcctacac cctggccgag    11340

ctcgactact tcatcggcga tgccgaaccg cgtctggtgg tcgttgctcc cgcagctcga    11400

ggtggcgtgg agacaattgc caagcgacac ggtgctatcg tcgaaaccct cgacgccgat    11460

ggacgaggct ccttgctgga ccttgctaga gatgagcctg ccgactttgt cgatgcttcg    11520

cgatctgccg acgatctggc tgctattctc tacacttccg gtacaaccgg acgatcgaag    11580

ggtgccatgc ttactcatgg caatctgctc tccaacgctc tcaccttgcg agactattgg    11640

agagttaccg cagacgatcg actcatccat gccttgccaa tctttcacac tcatggtctg    11700

ttcgttgcta cgaacgtcac actgcttgca ggagcctcga tgtttctgct ctccaagttc    11760

gatgccgacg aggtcgtttc tctcatgcca caggccacca tgcttatggg cgtgcccaca    11820

ttctacgttc gattgctgca gagtcctcga ctcgagaagg gtgctgtggc cagcatcaga    11880

ctgttcattt ctggatcagc tcccttgctt gccgaaaccc acgccgagtt tcatgctcgt    11940

actggtcacg ccattctcga gcgatacggc atgacggaaa ccaacatgaa tacttccaac    12000

ccctacgagg gcaagcgtat tgccggaacc gttggttttc ctctgcccga cgtcactgtg    12060

cgagtcaccg atcccgccac cggtctcgtt cttccacctg aagagactgg catgatcgag    12120

atcaagggac ccaacgtctt caagggctat tggcgaatgc ccgaaaagac cgctgccgag    12180

tttaccgcag acggtttctt tatctctgga gatctcggca agatcgaccg agaaggttac    12240

gttcacattg tgggacgagg caaggacctg gtcatttccg gtggctacaa catctatccc    12300

aaagaggtcg aaggcgagat cgaccagatc gagggtgtgg tcgagtctgc tgtcattggt    12360

gttcctcatc ccgatttcgg agaaggtgtc accgctgttg tcgtgtgcaa acctggtgcc    12420

gttctcgacg aaaagaccat cgtgtctgct ctgcaggacc gtcttgcccg atacaagcaa    12480

cccaagcgga ttatctttgc cgacgatctg cctcgaaaca ctatgggaaa ggttcagaag    12540

aacattcttc gacagcaata cgccgatctc tacaccagac gataagcggc cgcatgagaa    12600

gataaatata taaatacatt gagatattaa atgcgctaga ttagagagcc tcatactgct    12660

cggagagaag ccaagacgag tactcaaagg ggattacacc atccatatcc acagacacaa    12720

gctggggaaa ggttctatat acactttccg gaataccgta gtttccgatg ttatcaatgg    12780

gggcagccag gatttcaggc acttcggtgt ctcggggtga aatggcgttc ttggcctcca    12840

tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa cagaagcaga tgaagaatga    12900

acttgaagtg aaggaattta aatgtagcta acggtagcag gcgaactact ggtacatacc    12960

tcccccggaa tatgtacagg cataatgcgt atctgtggga catgtggtcg ttgcgccatt    13020

atgtaagcag cgtgtactcc tctgactgtc catatggttt gctccatctc accctcatcg    13080

ttttcattgt tcacaggcgg ccacaaaaaa actgtcttct ctccttctct cttcgcctta    13140

gtctactcgg accagtttta gtttagcttg gcgccactgg ataaatgaga cctcaggcct    13200

tgtgatgagg aggtcactta tgaagcatgt taggaggtgc ttgtatggat agagaagcac    13260

ccaaaataat aagaataata ataaaacagg gggcgttgtc atttcatatc gtgttttcac    13320

catcaataca cctccaaaca atgcccttca tgtggccagc cccaatattg tcctgtagtt    13380

caactctatg cagctcgtat cttattgagc aagtaaaact ctgtcagccg atattgcccg    13440

acccgcgaca agggtcaaca aggtggtgta aggccttcgc agaagtcaaa actgtgccaa    13500

acaaacatct agagtctctt tggtgtttct cgcatatatt twatcggctg tcttacgtat    13560

ttgcgcctcg gtaccggact aatttcggat catccccaat acgctttttc ttcgcagctg    13620

tcaacagtgt ccatgatcta tccacctaaa tgggtcatat gaggcgtata atttcgtggt    13680

gctgataata attcccatat atttgacaca aaacttcccc ccctagacat acatctcaca    13740

atctcacttc ttgtgcttct gtcacacatc tcctccagct gacttcaact cacacctctg    13800

ccccagttgg tctacagcgg tataaggttt ctccgcatag aggtgcacca ctcctcccga    13860

tacttgtttg tgtgacttgt gggtcacgac atatatatct acacacattg cgccaccctt    13920

tggttcttcc agcacaacaa aaacacgaca cgctaaccat ggagtctgga cccatgcctg    13980

ctggcattcc cttccctgag tactatgact tctttatgga ctggaagact cccctggcca    14040

tcgctgccac ctacactgct gccgtcggtc tcttcaaccc caaggttggc aaggtctccc    14100

gagtggttgc caagtcggct aacgcaaagc ctgccgagcg aacccagtcc ggagctgcca    14160

tgactgcctt cgtctttgtg cacaacctca ttctgtgtgt ctactctggc atcaccttct    14220

actacatgtt tcctgctatg gtcaagaact tccgaaccca cacactgcac gaagcctact    14280

gcgacacgga tcagtccctc tggaacaacg cacttggcta ctggggttac ctcttctacc    14340

tgtccaagtt ctacgaggtc attgacacca tcatcatcat cctgaaggga cgacggtcct    14400

cgctgcttca gacctaccac catgctggag ccatgattac catgtggtct ggcatcaact    14460

accaagccac tcccatttgg atctttgtgg tcttcaactc cttcattcac accatcatgt    14520

actgttacta tgccttcacc tctatcggat tccatcctcc tggcaaaaag tacctgactt    14580

cgatgcagat tactcagttt ctggtcggta tcaccattgc cgtgtcctac ctcttcgttc    14640

ctggctgcat ccgaacaccc ggtgctcaga tggctgtctg gatcaacgtc ggctacctgt    14700

ttcccttgac ctatctgttc gtggactttg ccaagcgaac ctactccaag cgatctgcca    14760

ttgccgctca gaaaaaggct cagtaagcgg ccgcaagtgt ggatggggaa gtgagtgccc    14820

ggttctgtgt gcacaattgg caatccaaga tggatggatt caacacaggg atatagcgag    14880

ctacgtggtg gtgcgaggat atagcaacgg atatttatgt ttgacacttg agaatgtacg    14940

atacaagcac tgtccaagta caatactaaa catactgtac atactcatac tcgtacccgg    15000

gcaacggttt cacttgagtg cagtggctag tgctcttact cgtacagtgt gcaatactgc    15060

gtatcatagt ctttgatgta tatcgtattc attcatgtta gttgc                    15105


<210>  90
<211>  828
<212>  DNA
<213>  Mortierella alpina


<220>
<221>  CDS
<222>  (1)..(828)
<223>  synthetic C16/18 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  A MORTIERELLA ALPINA C16/18 FATTY ACID ELONGASE
<310>  U.S. 7,470,532
<311>  2005-10-19
<312>  2008-12-30
<313>  (1)..(828)

<400>  90
atg gag tct gga ccc atg cct gct ggc att ccc ttc cct gag tac tat         48
Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr           
1               5                   10                  15                

gac ttc ttt atg gac tgg aag act ccc ctg gcc atc gct gcc acc tac         96
Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr           
            20                  25                  30                    

act gct gcc gtc ggt ctc ttc aac ccc aag gtt ggc aag gtc tcc cga        144
Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg           
        35                  40                  45                        

gtg gtt gcc aag tcg gct aac gca aag cct gcc gag cga acc cag tcc        192
Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser           
    50                  55                  60                            

gga gct gcc atg act gcc ttc gtc ttt gtg cac aac ctc att ctg tgt        240
Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys           
65                  70                  75                  80            

gtc tac tct ggc atc acc ttc tac tac atg ttt cct gct atg gtc aag        288
Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys           
                85                  90                  95                

aac ttc cga acc cac aca ctg cac gaa gcc tac tgc gac acg gat cag        336
Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln           
            100                 105                 110                   

tcc ctc tgg aac aac gca ctt ggc tac tgg ggt tac ctc ttc tac ctg        384
Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu           
        115                 120                 125                       

tcc aag ttc tac gag gtc att gac acc atc atc atc atc ctg aag gga        432
Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly           
    130                 135                 140                           

cga cgg tcc tcg ctg ctt cag acc tac cac cat gct gga gcc atg att        480
Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile           
145                 150                 155                 160           

acc atg tgg tct ggc atc aac tac caa gcc act ccc att tgg atc ttt        528
Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe           
                165                 170                 175               

gtg gtc ttc aac tcc ttc att cac acc atc atg tac tgt tac tat gcc        576
Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala           
            180                 185                 190                   

ttc acc tct atc gga ttc cat cct cct ggc aaa aag tac ctg act tcg        624
Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser           
        195                 200                 205                       

atg cag att act cag ttt ctg gtc ggt atc acc att gcc gtg tcc tac        672
Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr           
    210                 215                 220                           

ctc ttc gtt cct ggc tgc atc cga aca ccc ggt gct cag atg gct gtc        720
Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val           
225                 230                 235                 240           

tgg atc aac gtc ggc tac ctg ttt ccc ttg acc tat ctg ttc gtg gac        768
Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp           
                245                 250                 255               

ttt gcc aag cga acc tac tcc aag cga tct gcc att gcc gct cag aaa        816
Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys           
            260                 265                 270                   

aag gct cag taa                                                        828
Lys Ala Gln                                                               
        275                                                               


<210>  91
<211>  275
<212>  PRT
<213>  Mortierella alpina

<400>  91

Met Glu Ser Gly Pro Met Pro Ala Gly Ile Pro Phe Pro Glu Tyr Tyr 
1               5                   10                  15      


Asp Phe Phe Met Asp Trp Lys Thr Pro Leu Ala Ile Ala Ala Thr Tyr 
            20                  25                  30          


Thr Ala Ala Val Gly Leu Phe Asn Pro Lys Val Gly Lys Val Ser Arg 
        35                  40                  45              


Val Val Ala Lys Ser Ala Asn Ala Lys Pro Ala Glu Arg Thr Gln Ser 
    50                  55                  60                  


Gly Ala Ala Met Thr Ala Phe Val Phe Val His Asn Leu Ile Leu Cys 
65                  70                  75                  80  


Val Tyr Ser Gly Ile Thr Phe Tyr Tyr Met Phe Pro Ala Met Val Lys 
                85                  90                  95      


Asn Phe Arg Thr His Thr Leu His Glu Ala Tyr Cys Asp Thr Asp Gln 
            100                 105                 110         


Ser Leu Trp Asn Asn Ala Leu Gly Tyr Trp Gly Tyr Leu Phe Tyr Leu 
        115                 120                 125             


Ser Lys Phe Tyr Glu Val Ile Asp Thr Ile Ile Ile Ile Leu Lys Gly 
    130                 135                 140                 


Arg Arg Ser Ser Leu Leu Gln Thr Tyr His His Ala Gly Ala Met Ile 
145                 150                 155                 160 


Thr Met Trp Ser Gly Ile Asn Tyr Gln Ala Thr Pro Ile Trp Ile Phe 
                165                 170                 175     


Val Val Phe Asn Ser Phe Ile His Thr Ile Met Tyr Cys Tyr Tyr Ala 
            180                 185                 190         


Phe Thr Ser Ile Gly Phe His Pro Pro Gly Lys Lys Tyr Leu Thr Ser 
        195                 200                 205             


Met Gln Ile Thr Gln Phe Leu Val Gly Ile Thr Ile Ala Val Ser Tyr 
    210                 215                 220                 


Leu Phe Val Pro Gly Cys Ile Arg Thr Pro Gly Ala Gln Met Ala Val 
225                 230                 235                 240 


Trp Ile Asn Val Gly Tyr Leu Phe Pro Leu Thr Tyr Leu Phe Val Asp 
                245                 250                 255     


Phe Ala Lys Arg Thr Tyr Ser Lys Arg Ser Ala Ile Ala Ala Gln Lys 
            260                 265                 270         


Lys Ala Gln 
        275 


<210>  92
<211>  13237
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKMP-ML9DP

<400>  92
gtacgactga tgtgtatgta agctcaatga gcagcgtgct cctcggtcta ctatatggcg       60

acatatctct tcgcttctgt ttacccttta catacggtac agctcttaca atgacattta      120

tccactggcg tcgatccata aaccacacga accctgtttt gttagtcacc atgaccgggg      180

cgtgtcgtgt tacgttccac cgtttcacct cagccggtta cgattcaact tgccgcgtca      240

ttctgcgttg ctagcggagc gagtacgagt aactagactt tcgataagct gaatgacttc      300

agggtgcatg agaggcgcag atcggtattt tcggacttgt tcttctagaa gactggtttg      360

agagcaatcg cggaagagtt gggaaccgct ggagagcaga atgggttagt acagagacta      420

tctgatctcc cccgcttgtg tctagcccca ctttatactc tatttggcag ttgttgcttg      480

ttattcaagc acagcatgtc tgctcgtatc actttggaga catccctaac ctctaaacct      540

ttatagatgc cttaccttct gagcttgcag agagcattcc acagcgaacc cagttgacat      600

tatcagttga catcttacgc tcacaccaca ttacgcatct cacacacaat cacacgcaca      660

agtacacacg caagcacatc atacaatggt caaggcgcgc cagctgcatt aatgaatcgg      720

ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga      780

ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat      840

acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca      900

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc      960

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     1020

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     1080

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc     1140

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     1200

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     1260

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     1320

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     1380

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     1440

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     1500

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     1560

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat     1620

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga     1680

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg     1740

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga     1800

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc     1860

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac     1920

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc     1980

agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc     2040

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc     2100

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt     2160

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc     2220

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg     2280

tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag     2340

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat     2400

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc     2460

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa     2520

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta     2580

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa     2640

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg     2700

aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat     2760

tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga     2820

aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc     2880

agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac     2940

cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc     3000

gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg     3060

gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag     3120

ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc     3180

gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg     3240

gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta     3300

agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg     3360

taatacgact cactataggg cgaattgggc ccgacgtcgc atgctaggag agtctgtcgt     3420

tcgcgagtgg cgcgccaatg tcatgagtta cacctgattc aaatagtgaa taataatata     3480

acagccacaa atgaaagatg tatccacggg tagatatggc tttaattact gatgattgaa     3540

tgattatctg agtgctacag ttgtaacgag tacgagttat tacctgctac tattgtactc     3600

ctaaatcaaa gtactcgtac atgcaatgaa ttacttgtac ttgcgccaat cagatctatc     3660

ggttgtattg tatataatac tagtctattc ttttgcttac cagatggtgt aacctccatc     3720

gacaatgaga tcgtggccgg tcatgtagct ggaggcgtcg gaagccatga agacaatagg     3780

gcctccaaac tcctcgggct cagccattcg tcggaaagga attcgtcgct cccagtcgtt     3840

tcggagctct ccgtcagtct cgatgatgtg acgggtcaga ggagtaagaa tgtagccggg     3900

agacagagtg ttgactcgga tgttgtactg ggcccactcg gcagccagag acttggccat     3960

gtggatgaca cctgccttgg acatgttgta gggggtctgg ggctgggggt cgttgacaat     4020

ggagccactc atggatgcaa ccagaatcag agatccagga gactcgtcca ggacaagcga     4080

cttggccacc tcggtagcga agttgtagga tcccatgagg ttgacgtcca gcagtcgctt     4140

gacctggggg ttgggataat caaaggcagt catattctcg cagaatccgg cacagttaat     4200

taagttgcga cacatgtctt gatagtatct tgaattctct ctcttgagct tttccataac     4260

aagttcttct gcctccagga agtccatcgg tggtttgatc atggttttgg tgtagtggta     4320

gtgcagtggt ggtattgtga ctggggatgt agttgagaat aagtcataca caagtcagct     4380

ttcttcgagc ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc     4440

cgtatcgaga aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg     4500

cagtatcata catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc     4560

tccatacttg cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta     4620

acagttaatc ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat     4680

aggatctcgg ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga     4740

catgacatcc tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc     4800

caccccgggg gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat     4860

gaagccaacc acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc     4920

gccagtggcc agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag     4980

cttctcgttg ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac     5040

gtcctccttc ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat     5100

tccggttccg ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca     5160

ccggtactgg tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa     5220

gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc     5280

gtcaatgatg tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag     5340

ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc     5400

tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc     5460

gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt     5520

tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg     5580

aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc     5640

tctctgggcg tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt     5700

gcagctgata ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc     5760

caacgaagaa tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa     5820

aggcggcaat gacgagtcag acagatactc gtcgaccttt tccttgggaa ccaccaccgt     5880

cagcccttct gactcacgta ttgtagccac cgacacaggc aacagtccgt ggatagcaga     5940

atatgtcttg tcggtccatt tctcaccaac tttaggcgtc aagtgaatgt tgcagaagaa     6000

gtatgtgcct tcattgagaa tcggtgttgc tgatttcaat aaagtcttga gatcagtttg     6060

gccagtcatg ttgtgggggg taattggatt gagttatcgc ctacagtctg tacaggtata     6120

ctcgctgccc actttatact ttttgattcc gctgcacttg aagcaatgtc gtttaccaaa     6180

agtgagaatg ctccacagaa cacaccccag ggtatggttg agcaaaaaat aaacactccg     6240

atacggggaa tcgaaccccg gtctccacgg ttctcaagaa gtattcttga tgagagcgta     6300

tcgatggtta atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca ttgttgcgtt     6360

atgcagcgta caccacaata ttggaagctt attagccttt ctattttttc gtttgcaagg     6420

cttaacaaca ttgctgtgga gagggatggg gatatggagg ccgctggagg gagtcggaga     6480

ggcgttttgg agcggcttgg cctggcgccc agctcgcgaa acgcacctag gaccctttgg     6540

cacgccgaaa tgtgccactt ttcagtctag taacgcctta cctacgtcat tccatgcgtg     6600

catgtttgcg ccttttttcc cttgcccttg atcgccacac agtacagtgc actgtacagt     6660

ggaggttttg ggggggtctt agatgggagc taaaagcggc ctagcggtac actagtggga     6720

ttgtatggag tggcatggag cctaggtgga gcctgacagg acgcacgacc ggctagcccg     6780

tgacagacga tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc caaagtcttg     6840

tcagccttgc ttgcgaacct aattcccaat tttgtcactt cgcaccccca ttgatcgagc     6900

cctaacccct gcccatcagg caatccaatt aagctcgcat tgtctgcctt gtttagtttg     6960

gctcctgccc gtttcggcgt ccacttgcac aaacacaaac aagcattata tataaggctc     7020

gtctctccct cccaaccaca ctcacttttt tgcccgtctt cccttgctaa cacaaaagtc     7080

aagaacacaa acaaccaccc caaccccctt acacacaaga catatctaca gcaatggcca     7140

tggccaaaag caaacgacgg tcggaggctg tggaagagca cgtgaccggc tcggacgagg     7200

gcttgaccga tacttcgggt cacgtgagcc ctgccgccaa gaagcagaag aactcggaga     7260

ttcatttcac cacccaggct gcccagcagt tggatcggga gcgcaaggag gagtatctgg     7320

actcgctgat cgacaacaag gactatctca agtaccgtcc tcgaggctgg aagctcaaca     7380

acccgcctac cgaccgacct gtgcgaatct acgccgatgg agtgtttgat ttgttccatc     7440

tgggacacat gcgtcagctg gagcagtcca agaaggcctt ccccaacgca gtgttgattg     7500

tgggcattcc cagcgacaag gagacccaca agcggaaggg attgaccgtg ctgagtgacg     7560

tccagcggta cgagacggtg cgacactgca agtgggtgga cgaggtggtg gaggatgctc     7620

cctggtgtgt caccatggac tttctggaaa aacacaaaat cgactacgtg gcccatgacg     7680

atctgcccta cgcttccggc aacgacgatg atatctacaa gcccatcaag gagaagggca     7740

tgtttctggc cacccagcga accgagggca tttccacctc ggacatcatc accaagatta     7800

tccgagacta cgacaagtat ttaatgcgaa actttgcccg gggtgctaac cgaaaggatc     7860

tcaacgtctc gtggctcaag aagaacgagc tggacttcaa gcgtcatgtg gccgagttcc     7920

gaaactcgtt caagcgaaag aaggtcggta aggatctcta cggcgagatt cgcggtctgc     7980

tgcagaatgt gctcatttgg aacggcgaca actccggcac ttccactccc cagcgaaaga     8040

cgctgcagac caacgccaag aagatgtaca tgaacgtgct caagactctg caggctcctg     8100

acgctgttga cgtggactcc tcggagaacg tgtctgagaa cgtcactgat gaggaggagg     8160

aagacgacga cgaggttgat gaggacgaag aagccgacga cgacgacgaa gacgacgaag     8220

acgaggaaga cgacgagtag gcggccgcat tgatgattgg aaacacacac atgggttata     8280

tctaggtgag agttagttgg acagttatat attaaatcag ctatgccaac ggtaacttca     8340

ttcatgtcaa cgaggaacca gtgactgcaa gtaatataga atttgaccac cttgccattc     8400

tcttgcactc ctttactata tctcatttat ttcttatata caaatcactt cttcttccca     8460

gcatcgagct cggaaacctc atgagcaata acatcgtgga tctcgtcaat agagggcttt     8520

ttggactcct tgctgttggc caccttgtcc ttgctgttta aaccacgcca actgatatcc     8580

ccttacgtta ccccctcatc acctggtgag gcgaaactgt aaggtgaaag ctaaaaatga     8640

catctcagct gcacgaagga ccggggctta aaagacgggc tggtgcttgt gatttaaaac     8700

tggacaaatc tcagcttgct tgaaattttg gtctccaact gtttccgagc gaatcgcaca     8760

caaaccgggc ttctctctgc agaccacgcc cccgaaactc tttctcccac caccaccaac     8820

actccctttc cattcccaca ccgttcctct ctcgtgcgtc atccttgcgc aatcatcttc     8880

gtctgcgaca tattgtacga catacagtac cacggaacgt ttcagaccgt cgacgtgaac     8940

acatcttagg aacagcaacc tgagctacag aaatctatct ataggcggat aaaaaaacgc     9000

acccactgct cgtcctcctt gctcctcgaa accgactcct ctacacacgt caaatccgag     9060

gttgaaatct tccccacatt tggcagccaa accagcacat cccagcaacc tcgcacagcg     9120

ccgaaatcga cctgtcgact tggccacaaa aaaaagcacc ggctctgcaa cagttctcac     9180

gaccaattac gtacaagtac gaaatcgttc gtggaccgtg actgataagc tcccactttt     9240

tcttctaaca acaggcaaca gacaagtcac acaaaacaaa agccatggtg aaaaacgtgg     9300

accaagtgga tctctcgcag gtcgacacca ttgcctccgg ccgagatgtc aactacaagg     9360

tcaagtacac ctccggcgtt aagatgagcc agggcgccta cgacgacaag ggccgccaca     9420

tttccgagca gcccttcacc tgggccaact ggcaccagca catcaactgg ctcaacttca     9480

ttctggtgat tgcgctgcct ctgtcgtcct ttgctgccgc tcccttcgtc tccttcaact     9540

ggaagaccgc cgcgtttgct gtcggctatt acatgtgcac cggtctcggt atcaccgccg     9600

gctaccaccg aatgtgggcc catcgagcct acaaggccgc tctgcccgtt cgaatcatcc     9660

ttgctctgtt tggaggagga gctgtcgagg gctccatccg atggtgggcc tcgtctcacc     9720

gagtccacca ccgatggacc gactccaaca aggaccctta cgacgcccga aagggattct     9780

ggttctccca ctttggctgg atgctgcttg tgcccaaccc caagaacaag ggccgaactg     9840

acatttctga cctcaacaac gactgggttg tccgactcca gcacaagtac tacgtttacg     9900

ttctcgtctt catggccatt gttctgccca ccctcgtctg tggctttggc tggggcgact     9960

ggaagggagg tcttgtctac gccggtatca tgcgatacac ctttgtgcag caggtgactt    10020

tctgtgtcaa ctcccttgcc cactggattg gagagcagcc cttcgacgac cgacgaactc    10080

cccgagacca cgctcttacc gccctggtca cctttggaga gggctaccac aacttccacc    10140

acgagttccc ctcggactac cgaaacgccc tcatctggta ccagtacgac cccaccaagt    10200

ggctcatctg gaccctcaag caggttggtc tcgcctggga cctccagacc ttctcccaga    10260

acgccatcga gcagggtctc gtgcagcagc gacagaagaa gctggacaag tggcgaaaca    10320

acctcaactg gggtatcccc attgagcagc tgcctgtcat tgagtttgag gagttccaag    10380

agcaggccaa gacccgagat ctggttctca tttctggcat tgtccacgac gtgtctgcct    10440

ttgtcgagca ccaccctggt ggaaaggccc tcattatgag cgccgtcggc aaggacggta    10500

ccgctgtctt caacggaggt gtctaccgac actccaacgc tggccacaac ctgcttgcca    10560

ccatgcgagt ttcggtcatt cgaggcggca tggaggttga ggtgtggaag actgcccaga    10620

acgaaaagaa ggaccagaac attgtctccg atgagagtgg aaaccgaatc caccgagctg    10680

gtctccaggc cacccgggtc gagaaccccg gtatgtctgg catggctgct taggcggccg    10740

catgagaaga taaatatata aatacattga gatattaaat gcgctagatt agagagcctc    10800

atactgctcg gagagaagcc aagacgagta ctcaaagggg attacaccat ccatatccac    10860

agacacaagc tggggaaagg ttctatatac actttccgga ataccgtagt ttccgatgtt    10920

atcaatgggg gcagccagga tttcaggcac ttcggtgtct cggggtgaaa tggcgttctt    10980

ggcctccatc aagtcgtacc atgtcttcat ttgcctgtca aagtaaaaca gaagcagatg    11040

aagaatgaac ttgaagtgaa ggaatttaaa ttttcgagat tttacagata tttctcgcag    11100

tttttcacgt ccccttgtcc ttgtcctatt gtttcaaata aactctcgtc tactgatttc    11160

acatggaacc tttgctattt cggggataac cccctttgcc attgcacgat ggacgtggca    11220

aaagaaagat cgccctgcgg ggatacttat catgtggtca catgctgtga ttagaaataa    11280

agaaaaaggt gcttttttgg cgctgtgatt aacatctcgt ctgccgtgct ctactagtcg    11340

caatagcaaa aactcgctta atagtgtgca tagtgcgggg tagcaggata ctgaactaca    11400

gtacgatttg cttgctactg cttgtagcaa ttacctttac tgtagggacc acacctcctg    11460

gtttcaatgt ctttcctcgc ctcgacaaag caaaactgtc acccaatcac accttgttca    11520

tattcattag tgcatccgtt aaccttgaca tgacacttct catactagtg atagggctgt    11580

agttgagaca agttgattca cacggataca tacaaagcct cagagagcaa atgttatata    11640

ctcagggacc gaccaatcaa aaaaacacac tcctaataac caccatttcc atctacgcgt    11700

actcactctg tcagctgccc cacattgccc aatgcacaat gcacaatgat gtgtgcaaac    11760

aacgcaatca aaagtctatg gatgctgacc aaactctgat caccaagttg cgaacatgaa    11820

aaagaagacc tgtgtatata taagtaaggg ggagagccct aactagatct ttcgaaaacc    11880

ccccgacctt caccttccac aaccatgatc atcttatacg ttttggccgt tgcggtctcc    11940

ttcctcatct tcaagagagt cacctacacc atggcttcta ttggttcgtc caaccccgtg    12000

ctcttggctg cgattccctt cgtctacctg tttgtcctcc cacgagtcct ggctttcctg    12060

cctcagaagg ctcagttcct ggccaaatgt attgtggtcc tgattgccac gcttatcatg    12120

tccgttgcag gctgcttcat ctcgatcgtg tgcgctcttc tggacaagag atacgtcatc    12180

aattacgttg tgtcgcgatt gttctccttc cttgccgctc gaccgtgtgg tgtgacctat    12240

aagattgttg gtgaggaaca cctcgataag taccctgcta tcgtggtctg taaccatcaa    12300

tcctctatgg atatgatggt tttgggacga gtttttccaa agcactgcgt tgtcatggcg    12360

aagaaggaac tcctgtactt tccctttttg ggaatgttta tgaaactgag caacgctatc    12420

ttcatcgacc ggaagaacca caagaaagcc atcgagtcta ccacccaagc cgtggcggac    12480

atgaagaagc acaactctgg aatctggatt ttcccagagg gcacccggtc tagactggac    12540

aaggcagacc tgctgccctt caagaaaggt gcctttcatc ttgcaattca ggcccagctc    12600

cctattctcc ccattatctc gcagggctat tcccatatct acgactcttc gaagcggtac    12660

ttccccggtg gagagctcga gatcagagtc ctggagccca ttcctacaac tggcctcact    12720

actgatgatg tgaacgacct gatggacaag acacgaaacc ttatgctcaa gcacttgaag    12780

gagatggatt cccagtattc gtcgagcact gctgaaaatg gatccacgca catcgacgcc    12840

gatattgcca agtctacagc caccagcatt ggcaacactg acgacgcaat tacaaaacgt    12900

cgtaccccta aggaataagc ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt    12960

gtgcacaatt ggcaatccaa gatggatgga ttcaacacag ggatatagcg agctacgtgg    13020

tggtgcgagg atatagcaac ggatatttat gtttgacact tgagaatgta cgatacaagc    13080

actgtccaag tacaatacta aacatactgt acatactcat actcgtaccc gggcaacggt    13140

ttcacttgag tgcagtggct agtgctctta ctcgtacagt gtgcaatact gcgtatcata    13200

gtctttgatg tatatcgtat tcattcatgt tagttgc                             13237


<210>  93
<211>  899
<212>  DNA
<213>  Yarrowia lipolytica

<400>  93
tttcgagatt ttacagatat ttctcgcagt ttttcacgtc cccttgtcct tgtcctattg       60

tttcaaataa actctcgtct actgatttca catggaacct ttgctatttc ggggataacc      120

ccctttgcca ttgcacgatg gacgtggcaa aagaaagatc gccctgcggg gatacttatc      180

atgtggtcac atgctgtgat tagaaataaa gaaaaaggtg cttttttggc gctgtgatta      240

acatctcgtc tgccgtgctc tactagtcgc aatagcaaaa actcgcttaa tagtgtgcat      300

agtgcggggt agcaggatac tgaactacag tacgatttgc ttgctactgc ttgtagcaat      360

tacctttact gtagggacca cacctcctgg tttcaatgtc tttcctcgcc tcgacaaagc      420

aaaactgtca cccaatcaca ccttgttcat attcattagt gcatccgtta accttgacat      480

gacacttctc atactagtga tagggctgta gttgagacaa gttgattcac acggatacat      540

acaaagcctc agagagcaaa tgttatatac tcagggaccg accaatcaaa aaaacacact      600

cctaataacc accatttcca tctacgcgta ctcactctgt cagctgcccc acattgccca      660

atgcacaatg cacaatgatg tgtgcaaaca acgcaatcaa aagtctatgg atgctgacca      720

aactctgatc accaagttgc gaacatgaaa aagaagacct gtgtatatat aagtaagggg      780

gagagcccta actagatctt tcgaaaaccc cccgaccttc accttccaca accatgatca      840

tcttatacgt tttggccgtt gcggtctcct tcctcatctt caagagagtc acctacacc       899


<210>  94
<211>  722
<212>  DNA
<213>  Yarrowia lipolytica

<400>  94
ccacgccaac tgatatcccc ttacgttacc ccctcatcac ctggtgaggc gaaactgtaa       60

ggtgaaagct aaaaatgaca tctcagctgc acgaaggacc ggggcttaaa agacgggctg      120

gtgcttgtga tttaaaactg gacaaatctc agcttgcttg aaattttggt ctccaactgt      180

ttccgagcga atcgcacaca aaccgggctt ctctctgcag accacgcccc cgaaactctt      240

tctcccacca ccaccaacac tccctttcca ttcccacacc gttcctctct cgtgcgtcat      300

ccttgcgcaa tcatcttcgt ctgcgacata ttgtacgaca tacagtacca cggaacgttt      360

cagaccgtcg acgtgaacac atcttaggaa cagcaacctg agctacagaa atctatctat      420

aggcggataa aaaaacgcac ccactgctcg tcctccttgc tcctcgaaac cgactcctct      480

acacacgtca aatccgaggt tgaaatcttc cccacatttg gcagccaaac cagcacatcc      540

cagcaacctc gcacagcgcc gaaatcgacc tgtcgacttg gccacaaaaa aaagcaccgg      600

ctctgcaaca gttctcacga ccaattacgt acaagtacga aatcgttcgt ggaccgtgac      660

tgataagctc ccactttttc ttctaacaac aggcaacaga caagtcacac aaaacaaaag      720

cc                                                                     722


<210>  95
<211>  13320
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKMP-ML9DCB

<400>  95
cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt tggcgctcat tgttgcgtta       60

tgcagcgtac accacaatat tggaagctta ttagcctttc tattttttcg tttgcaaggc      120

ttaacaacat tgctgtggag agggatgggg atatggaggc cgctggaggg agtcggagag      180

gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa cgcacctagg accctttggc      240

acgccgaaat gtgccacttt tcagtctagt aacgccttac ctacgtcatt ccatgcgtgc      300

atgtttgcgc cttttttccc ttgcccttga tcgccacaca gtacagtgca ctgtacagtg      360

gaggttttgg gggggtctta gatgggagct aaaagcggcc tagcggtaca ctagtgggat      420

tgtatggagt ggcatggagc ctaggtggag cctgacagga cgcacgaccg gctagcccgt      480

gacagacgat gggtggctcc tgttgtccac cgcgtacaaa tgtttgggcc aaagtcttgt      540

cagccttgct tgcgaaccta attcccaatt ttgtcacttc gcacccccat tgatcgagcc      600

ctaacccctg cccatcaggc aatccaatta agctcgcatt gtctgccttg tttagtttgg      660

ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca agcattatat ataaggctcg      720

tctctccctc ccaaccacac tcactttttt gcccgtcttc ccttgctaac acaaaagtca      780

agaacacaaa caaccacccc aaccccctta cacacaagac atatctacag caatggccat      840

gggcgtattc attaaacagg agcagcttcc ggctctcaag aagtacaagt actccgccga      900

ggatcactcg ttcatctcca acaacattct gcgccccttc tggcgacagt ttgtcaaaat      960

cttccctctg tggatggccc ccaacatggt gactctgctg ggcttcttct ttgtcattgt     1020

gaacttcatc accatgctca ttgttgatcc cacccacgac cgcgagcctc ccagatgggt     1080

ctacctcacc tacgctctgg gtctgttcct ttaccagaca tttgatgcct gtgacggatc     1140

ccatgcccga cgaactggcc agagtggacc ccttggagag ctgtttgacc actgtgtcga     1200

cgccatgaat acctctctga ttctcacggt ggtggtgtcc accacccata tgggatataa     1260

catgaagcta ctgattgtgc agattgccgc tctcggaaac ttctacctgt cgacctggga     1320

gacctaccat accggaactc tgtacctttc tggcttctct ggtcctgttg aaggtatctt     1380

gattctggtg gctcttttcg tcctcacctt cttcactggt cccaacgtgt acgctctgac     1440

cgtctacgag gctcttcccg agtccatcac ttcgctgctg cctgccagct tcctggacgt     1500

caccatcacc cagatctaca ttggattcgg agtgctgggc atggtgttca acatctacgg     1560

cgcctgcgga aacgtgatca agtactacaa caacaagggc aagagcgctc tccccgccat     1620

tctcggaatc gccccctttg gcatcttcta cgtcggcgtc tttgcctggg cccatgttgc     1680

tcctctgctt ctctccaagt acgccatcgt ctatctgttt gccattgggg ctgcctttgc     1740

catgcaagtc ggccagatga ttcttgccca tctcgtgctt gctccctttc cccactggaa     1800

cgtgctgctc ttcttcccct ttgtgggact ggcagtgcac tacattgcac ccgtgtttgg     1860

ctgggacgcc gatatcgtgt cggttaacac tctcttcacc tgttttggcg ccaccctctc     1920

catttacgcc ttctttgtgc ttgagatcat cgacgagatc accaactacc tcgatatctg     1980

gtgtctgcga atcaagtacc ctcaggagaa gaagaccgaa taagcggccg catgtacata     2040

caagattatt tatagaaatg aatcgcgatc gaacaaagag tacgagtgta cgagtagggg     2100

atgatgataa aagtggaaga agttccgcat ctttggattt atcaacgtgt aggacgatac     2160

ttcctgtaaa aatgcaatgt ctttaccata ggttctgctg tagatgttat taactaccat     2220

taacatgtct acttgtacag ttgcagacca gttggagtat agaatggtac acttaccaaa     2280

aagtgttgat ggttgtaact acgatatata aaactgttga cgggatctgc gtacactgtt     2340

taaaccacgc caactgatat ccccttacgt taccccctca tcacctggtg aggcgaaact     2400

gtaaggtgaa agctaaaaat gacatctcag ctgcacgaag gaccggggct taaaagacgg     2460

gctggtgctt gtgatttaaa actggacaaa tctcagcttg cttgaaattt tggtctccaa     2520

ctgtttccga gcgaatcgca cacaaaccgg gcttctctct gcagaccacg cccccgaaac     2580

tctttctccc accaccacca acactccctt tccattccca caccgttcct ctctcgtgcg     2640

tcatccttgc gcaatcatct tcgtctgcga catattgtac gacatacagt accacggaac     2700

gtttcagacc gtcgacgtga acacatctta ggaacagcaa cctgagctac agaaatctat     2760

ctataggcgg ataaaaaaac gcacccactg ctcgtcctcc ttgctcctcg aaaccgactc     2820

ctctacacac gtcaaatccg aggttgaaat cttccccaca tttggcagcc aaaccagcac     2880

atcccagcaa cctcgcacag cgccgaaatc gacctgtcga cttggccaca aaaaaaagca     2940

ccggctctgc aacagttctc acgaccaatt acgtacaagt acgaaatcgt tcgtggaccg     3000

tgactgataa gctcccactt tttcttctaa caacaggcaa cagacaagtc acacaaaaca     3060

aaagccatgg tgaaaaacgt ggaccaagtg gatctctcgc aggtcgacac cattgcctcc     3120

ggccgagatg tcaactacaa ggtcaagtac acctccggcg ttaagatgag ccagggcgcc     3180

tacgacgaca agggccgcca catttccgag cagcccttca cctgggccaa ctggcaccag     3240

cacatcaact ggctcaactt cattctggtg attgcgctgc ctctgtcgtc ctttgctgcc     3300

gctcccttcg tctccttcaa ctggaagacc gccgcgtttg ctgtcggcta ttacatgtgc     3360

accggtctcg gtatcaccgc cggctaccac cgaatgtggg cccatcgagc ctacaaggcc     3420

gctctgcccg ttcgaatcat ccttgctctg tttggaggag gagctgtcga gggctccatc     3480

cgatggtggg cctcgtctca ccgagtccac caccgatgga ccgactccaa caaggaccct     3540

tacgacgccc gaaagggatt ctggttctcc cactttggct ggatgctgct tgtgcccaac     3600

cccaagaaca agggccgaac tgacatttct gacctcaaca acgactgggt tgtccgactc     3660

cagcacaagt actacgttta cgttctcgtc ttcatggcca ttgttctgcc caccctcgtc     3720

tgtggctttg gctggggcga ctggaaggga ggtcttgtct acgccggtat catgcgatac     3780

acctttgtgc agcaggtgac tttctgtgtc aactcccttg cccactggat tggagagcag     3840

cccttcgacg accgacgaac tccccgagac cacgctctta ccgccctggt cacctttgga     3900

gagggctacc acaacttcca ccacgagttc ccctcggact accgaaacgc cctcatctgg     3960

taccagtacg accccaccaa gtggctcatc tggaccctca agcaggttgg tctcgcctgg     4020

gacctccaga ccttctccca gaacgccatc gagcagggtc tcgtgcagca gcgacagaag     4080

aagctggaca agtggcgaaa caacctcaac tggggtatcc ccattgagca gctgcctgtc     4140

attgagtttg aggagttcca agagcaggcc aagacccgag atctggttct catttctggc     4200

attgtccacg acgtgtctgc ctttgtcgag caccaccctg gtggaaaggc cctcattatg     4260

agcgccgtcg gcaaggacgg taccgctgtc ttcaacggag gtgtctaccg acactccaac     4320

gctggccaca acctgcttgc caccatgcga gtttcggtca ttcgaggcgg catggaggtt     4380

gaggtgtgga agactgccca gaacgaaaag aaggaccaga acattgtctc cgatgagagt     4440

ggaaaccgaa tccaccgagc tggtctccag gccacccggg tcgagaaccc cggtatgtct     4500

ggcatggctg cttaggcggc cgcatgagaa gataaatata taaatacatt gagatattaa     4560

atgcgctaga ttagagagcc tcatactgct cggagagaag ccaagacgag tactcaaagg     4620

ggattacacc atccatatcc acagacacaa gctggggaaa ggttctatat acactttccg     4680

gaataccgta gtttccgatg ttatcaatgg gggcagccag gatttcaggc acttcggtgt     4740

ctcggggtga aatggcgttc ttggcctcca tcaagtcgta ccatgtcttc atttgcctgt     4800

caaagtaaaa cagaagcaga tgaagaatga acttgaagtg aaggaattta aattttcgag     4860

attttacaga tatttctcgc agtttttcac gtccccttgt ccttgtccta ttgtttcaaa     4920

taaactctcg tctactgatt tcacatggaa cctttgctat ttcggggata accccctttg     4980

ccattgcacg atggacgtgg caaaagaaag atcgccctgc ggggatactt atcatgtggt     5040

cacatgctgt gattagaaat aaagaaaaag gtgctttttt ggcgctgtga ttaacatctc     5100

gtctgccgtg ctctactagt cgcaatagca aaaactcgct taatagtgtg catagtgcgg     5160

ggtagcagga tactgaacta cagtacgatt tgcttgctac tgcttgtagc aattaccttt     5220

actgtaggga ccacacctcc tggtttcaat gtctttcctc gcctcgacaa agcaaaactg     5280

tcacccaatc acaccttgtt catattcatt agtgcatccg ttaaccttga catgacactt     5340

ctcatactag tgatagggct gtagttgaga caagttgatt cacacggata catacaaagc     5400

ctcagagagc aaatgttata tactcaggga ccgaccaatc aaaaaaacac actcctaata     5460

accaccattt ccatctacgc gtactcactc tgtcagctgc cccacattgc ccaatgcaca     5520

atgcacaatg atgtgtgcaa acaacgcaat caaaagtcta tggatgctga ccaaactctg     5580

atcaccaagt tgcgaacatg aaaaagaaga cctgtgtata tataagtaag ggggagagcc     5640

ctaactagat ctttcgaaaa ccccccgacc ttcaccttcc acaaccatga tcatcttata     5700

cgttttggcc gttgcggtct ccttcctcat cttcaagaga gtcacctaca ccatggcttc     5760

tattggttcg tccaaccccg tgctcttggc tgcgattccc ttcgtctacc tgtttgtcct     5820

cccacgagtc ctggctttcc tgcctcagaa ggctcagttc ctggccaaat gtattgtggt     5880

cctgattgcc acgcttatca tgtccgttgc aggctgcttc atctcgatcg tgtgcgctct     5940

tctggacaag agatacgtca tcaattacgt tgtgtcgcga ttgttctcct tccttgccgc     6000

tcgaccgtgt ggtgtgacct ataagattgt tggtgaggaa cacctcgata agtaccctgc     6060

tatcgtggtc tgtaaccatc aatcctctat ggatatgatg gttttgggac gagtttttcc     6120

aaagcactgc gttgtcatgg cgaagaagga actcctgtac tttccctttt tgggaatgtt     6180

tatgaaactg agcaacgcta tcttcatcga ccggaagaac cacaagaaag ccatcgagtc     6240

taccacccaa gccgtggcgg acatgaagaa gcacaactct ggaatctgga ttttcccaga     6300

gggcacccgg tctagactgg acaaggcaga cctgctgccc ttcaagaaag gtgcctttca     6360

tcttgcaatt caggcccagc tccctattct ccccattatc tcgcagggct attcccatat     6420

ctacgactct tcgaagcggt acttccccgg tggagagctc gagatcagag tcctggagcc     6480

cattcctaca actggcctca ctactgatga tgtgaacgac ctgatggaca agacacgaaa     6540

ccttatgctc aagcacttga aggagatgga ttcccagtat tcgtcgagca ctgctgaaaa     6600

tggatccacg cacatcgacg ccgatattgc caagtctaca gccaccagca ttggcaacac     6660

tgacgacgca attacaaaac gtcgtacccc taaggaataa gcggccgcaa gtgtggatgg     6720

ggaagtgagt gcccggttct gtgtgcacaa ttggcaatcc aagatggatg gattcaacac     6780

agggatatag cgagctacgt ggtggtgcga ggatatagca acggatattt atgtttgaca     6840

cttgagaatg tacgatacaa gcactgtcca agtacaatac taaacatact gtacatactc     6900

atactcgtac ccgggcaacg gtttcacttg agtgcagtgg ctagtgctct tactcgtaca     6960

gtgtgcaata ctgcgtatca tagtctttga tgtatatcgt attcattcat gttagttgcg     7020

tacgactgat gtgtatgtaa gctcaatgag cagcgtgctc ctcggtctac tatatggcga     7080

catatctctt cgcttctgtt taccctttac atacggtaca gctcttacaa tgacatttat     7140

ccactggcgt cgatccataa accacacgaa ccctgttttg ttagtcacca tgaccggggc     7200

gtgtcgtgtt acgttccacc gtttcacctc agccggttac gattcaactt gccgcgtcat     7260

tctgcgttgc tagcggagcg agtacgagta actagacttt cgataagctg aatgacttca     7320

gggtgcatga gaggcgcaga tcggtatttt cggacttgtt cttctagaag actggtttga     7380

gagcaatcgc ggaagagttg ggaaccgctg gagagcagaa tgggttagta cagagactat     7440

ctgatctccc ccgcttgtgt ctagccccac tttatactct atttggcagt tgttgcttgt     7500

tattcaagca cagcatgtct gctcgtatca ctttggagac atccctaacc tctaaacctt     7560

tatagatgcc ttaccttctg agcttgcaga gagcattcca cagcgaaccc agttgacatt     7620

atcagttgac atcttacgct cacaccacat tacgcatctc acacacaatc acacgcacaa     7680

gtacacacgc aagcacatca tacaatggtc aaggcgcgcc agctgcatta atgaatcggc     7740

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac     7800

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     7860

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     7920

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     7980

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     8040

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     8100

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     8160

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     8220

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     8280

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     8340

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga     8400

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     8460

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     8520

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     8580

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc     8640

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     8700

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     8760

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     8820

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     8880

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     8940

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     9000

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     9060

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     9120

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     9180

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     9240

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     9300

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     9360

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     9420

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     9480

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     9540

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat     9600

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa     9660

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga     9720

aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt     9780

ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa     9840

atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca     9900

gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc     9960

gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg    10020

aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg    10080

ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg    10140

gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg    10200

ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg    10260

tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa    10320

gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt    10380

aatacgactc actatagggc gaattgggcc cgacgtcgca tgctaggaga gtctgtcgtt    10440

cgcgagtggc gcgccaatgt catgagttac acctgattca aatagtgaat aataatataa    10500

cagccacaaa tgaaagatgt atccacgggt agatatggct ttaattactg atgattgaat    10560

gattatctga gtgctacagt tgtaacgagt acgagttatt acctgctact attgtactcc    10620

taaatcaaag tactcgtaca tgcaatgaat tacttgtact tgcgccaatc agatctatcg    10680

gttgtattgt atataatact agtctattct tttgcttacc agatggtgta acctccatcg    10740

acaatgagat cgtggccggt catgtagctg gaggcgtcgg aagccatgaa gacaataggg    10800

cctccaaact cctcgggctc agccattcgt cggaaaggaa ttcgtcgctc ccagtcgttt    10860

cggagctctc cgtcagtctc gatgatgtga cgggtcagag gagtaagaat gtagccggga    10920

gacagagtgt tgactcggat gttgtactgg gcccactcgg cagccagaga cttggccatg    10980

tggatgacac ctgccttgga catgttgtag ggggtctggg gctgggggtc gttgacaatg    11040

gagccactca tggatgcaac cagaatcaga gatccaggag actcgtccag gacaagcgac    11100

ttggccacct cggtagcgaa gttgtaggat cccatgaggt tgacgtccag cagtcgcttg    11160

acctgggggt tgggataatc aaaggcagtc atattctcgc agaatccggc acagttaatt    11220

aagttgcgac acatgtcttg atagtatctt gaattctctc tcttgagctt ttccataaca    11280

agttcttctg cctccaggaa gtccatcggt ggtttgatca tggttttggt gtagtggtag    11340

tgcagtggtg gtattgtgac tggggatgta gttgagaata agtcatacac aagtcagctt    11400

tcttcgagcc tcatataagt ataagtagtt caacgtatta gcactgtacc cagcatctcc    11460

gtatcgagaa acacaacaac atgccccatt ggacagatca tgcggataca caggttgtgc    11520

agtatcatac atactcgatc agacaggtcg tctgaccatc atacaagctg aacaagcgct    11580

ccatacttgc acgctctcta tatacacagt taaattacat atccatagtc taacctctaa    11640

cagttaatct tctggtaagc ctcccagcca gccttctggt atcgcttggc ctcctcaata    11700

ggatctcggt tctggccgta cagacctcgg ccgacaatta tgatatccgt tccggtagac    11760

atgacatcct caacagttcg gtactgctgt ccgagagcgt ctcccttgtc gtcaagaccc    11820

accccggggg tcagaataag ccagtcctca gagtcgccct taggtcggtt ctgggcaatg    11880

aagccaacca caaactcggg gtcggatcgg gcaagctcaa tggtctgctt ggagtactcg    11940

ccagtggcca gagagccctt gcaagacagc tcggccagca tgagcagacc tctggccagc    12000

ttctcgttgg gagaggggac taggaactcc ttgtactggg agttctcgta gtcagagacg    12060

tcctccttct tctgttcaga gacagtttcc tcggcaccag ctcgcaggcc agcaatgatt    12120

ccggttccgg gtacaccgtg ggcgttggtg atatcggacc actcggcgat tcggtgacac    12180

cggtactggt gcttgacagt gttgccaata tctgcgaact ttctgtcctc gaacaggaag    12240

aaaccgtgct taagagcaag ttccttgagg gggagcacag tgccggcgta ggtgaagtcg    12300

tcaatgatgt cgatatgggt tttgatcatg cacacataag gtccgacctt atcggcaagc    12360

tcaatgagct ccttggtggt ggtaacatcc agagaagcac acaggttggt tttcttggct    12420

gccacgagct tgagcactcg agcggcaaag gcggacttgt ggacgttagc tcgagcttcg    12480

taggagggca ttttggtggt gaagaggaga ctgaaataaa tttagtctgc agaacttttt    12540

atcggaacct tatctggggc agtgaagtat atgttatggt aatagttacg agttagttga    12600

acttatagat agactggact atacggctat cggtccaaat tagaaagaac gtcaatggct    12660

ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat gaaagccagc aatgacgttg    12720

cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag ctgtcagacc cacagcctcc    12780

aacgaagaat gtatcgtcaa agtgatccaa gcacactcat agttggagtc gtactccaaa    12840

ggcggcaatg acgagtcaga cagatactcg tcgacctttt ccttgggaac caccaccgtc    12900

agcccttctg actcacgtat tgtagccacc gacacaggca acagtccgtg gatagcagaa    12960

tatgtcttgt cggtccattt ctcaccaact ttaggcgtca agtgaatgtt gcagaagaag    13020

tatgtgcctt cattgagaat cggtgttgct gatttcaata aagtcttgag atcagtttgg    13080

ccagtcatgt tgtggggggt aattggattg agttatcgcc tacagtctgt acaggtatac    13140

tcgctgccca ctttatactt tttgattccg ctgcacttga agcaatgtcg tttaccaaaa    13200

gtgagaatgc tccacagaac acaccccagg gtatggttga gcaaaaaata aacactccga    13260

tacggggaat cgaaccccgg tctccacggt tctcaagaag tattcttgat gagagcgtat    13320


<210>  96
<211>  13975
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKSL-5S5A5

<400>  96
cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt tggcgctcat tgttgcgtta       60

tgcagcgtac accacaatat tggaagctta ttagcctttc tattttttcg tttgcaaggc      120

ttaacaacat tgctgtggag agggatgggg atatggaggc cgctggaggg agtcggagag      180

gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa cgcacctagg accctttggc      240

acgccgaaat gtgccacttt tcagtctagt aacgccttac ctacgtcatt ccatgcgtgc      300

atgtttgcgc cttttttccc ttgcccttga tcgccacaca gtacagtgca ctgtacagtg      360

gaggttttgg gggggtctta gatgggagct aaaagcggcc tagcggtaca ctagtgggat      420

tgtatggagt ggcatggagc ctaggtggag cctgacagga cgcacgaccg gctagcccgt      480

gacagacgat gggtggctcc tgttgtccac cgcgtacaaa tgtttgggcc aaagtcttgt      540

cagccttgct tgcgaaccta attcccaatt ttgtcacttc gcacccccat tgatcgagcc      600

ctaacccctg cccatcaggc aatccaatta agctcgcatt gtctgccttg tttagtttgg      660

ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca agcattatat ataaggctcg      720

tctctccctc ccaaccacac tcactttttt gcccgtcttc ccttgctaac acaaaagtca      780

agaacacaaa caaccacccc aaccccctta cacacaagac atatctacag caatggccat      840

ggctctcagt cttaccacag aacagctgtt agaacgccct gatttggttg cgattgatgg      900

catcctctac gaccttgaag ggcttgccaa agttcatcca ggatccgatt tgattctcgc      960

ttctggtgcc tctgatgcct cccctctctt ttattcaatg catccatacg tcaaaccgga     1020

gaactccaaa ttgcttcaac agttcgtccg agggaagcat gaccgcacct cgaaggacat     1080

tgtctacacg tatgattctc ccttcgcaca agacgttaag cggacaatgc gcgaggtgat     1140

gaaagggagg aactggtacg caacccctgg cttctggctg cgcaccgttg ggatcatcgc     1200

cgtgacggcc ttttgcgagt ggcactgggc taccacgggg atggtgctgt ggggcctgtt     1260

gactggattc atgcacatgc agatcggctt atccatccag catgatgcgt cccacggggc     1320

catcagcaag aagccttggg tcaacgccct cttcgcctac ggcattgacg tcatcggatc     1380

gtcccggtgg atttggctgc agtcgcacat catgcggcac cacacctaca ccaaccagca     1440

cggcctcgac ctggatgcgg agtcggcaga gccgttcctg gtgttccaca actaccccgc     1500

cgcaaacacc gcccgaaagt ggttccaccg cttccaggct tggtacatgt accttgtgct     1560

gggggcatac ggggtatcgc tggtgtacaa cccgctctac attttccgga tgcagcacaa     1620

tgacaccatc ccagagtctg tcacggccat gcgggaaaat ggctttctgc ggcgctaccg     1680

cacacttgca ttcgtgatgc gagctttctt catcttccgg accgcattct tgccctggta     1740

cctcactggg acctcattgc tgatcaccat tcctctggtg cccaccgcaa ctggtgcctt     1800

cttgacgttc ttcttcattt tgtcccacaa ttttgatggc tccgaacgga tccccgacaa     1860

gaactgcaag gttaagcgat ctgagaagga cgttgaggct gaccaaattg actggtatcg     1920

ggcgcaggtg gagacgtcct ccacatacgg tggccccatc gccatgttct tcactggcgg     1980

tctcaatttc cagatcgagc accacctctt tccccggatg tcgtcttggc actacccctt     2040

cgtccagcag gcggtccggg agtgttgcga acgacatgga gtgcgatatg ttttctaccc     2100

taccatcgtc ggcaacatca tctccaccct gaagtacatg cataaggtgg gtgtcgtcca     2160

ctgcgtgaag gacgcacagg attcctaagc ggccgcattg atgattggaa acacacacat     2220

gggttatatc taggtgagag ttagttggac agttatatat taaatcagct atgccaacgg     2280

taacttcatt catgtcaacg aggaaccagt gactgcaagt aatatagaat ttgaccacct     2340

tgccattctc ttgcactcct ttactatatc tcatttattt cttatataca aatcacttct     2400

tcttcccagc atcgagctcg gaaacctcat gagcaataac atcgtggatc tcgtcaatag     2460

agggcttttt ggactccttg ctgttggcca ccttgtcctt gctgtctggc tcattctgtt     2520

tcaacgcctt ttaattaact ctcctgcagc ttcctcagcg agacactgct cctgtctgga     2580

cccgagctaa ggctctgttc gacaaacacg ttctgcgaat tggcgagtaa tttcattaat     2640

gcaaatagac gtgtatttga aaaggaggag atgatggtac cagtaattta cggtgtttga     2700

atagacaaat tatatatata aaattaacct gatgaatgag tgtatgaatg gattgcttaa     2760

tataccgagg gagagccggc attatcaata tttattgtcc taagaagcta aaatatgctg     2820

ttccgttgat agcgatgtct gtttgagagt caatggcaga acgcggagga gtattgttag     2880

ttggtgatcg gtttactcga catcgagtag ggggcgagac agaagatatc ccgaatccat     2940

tgcattgttt attaggatgt tcacaacaca actccctcta gactctgggg atgtgcgtta     3000

gaagaatgac ctggagcaag agtgttcaga ttgatccgtt caaattttca agattactgt     3060

tggggttgtt tttgaatcca cattagctgg acgactattg catctgagcg ctcggaacgt     3120

ctccctttcc cttcagcttt atacgagtcg attccataag cgcgacctct cgaattgcct     3180

tcggttgtga agccataggc aaaggtgtgg ctatggaatg catgcgacgt cgggcccaat     3240

tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg     3300

gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg     3360

cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc     3420

gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     3480

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     3540

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc     3600

gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta     3660

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     3720

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg     3780

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa     3840

aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct     3900

ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc     3960

gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac     4020

aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt     4080

tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag     4140

aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg     4200

aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa     4260

tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc     4320

aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag     4380

tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa     4440

ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc     4500

taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg     4560

agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa     4620

caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa     4680

tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg     4740

gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag     4800

cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg     4860

caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt     4920

ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt     4980

aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac     5040

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     5100

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     5160

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     5220

gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga     5280

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     5340

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     5400

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     5460

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     5520

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     5580

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     5640

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     5700

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     5760

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     5820

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca     5880

aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcgcgcc gaggtttcca     5940

acgagataac atcgtggcag ctgccaccat gaccgatatg cctgaaataa gtgaattgac     6000

catcagaagt tctgcattca aatagaacta tatcatattc ggctcagttt tttcaataat     6060

agtccaatcc ctaagtctcc tatccaaaat ggttcctgac cagccacatc catgatcatg     6120

actgcgtgac aggaacagtc attccgtgga tgaacgactt tacgctcagg tactgtaaat     6180

atctgtaaag ggcagacaac caaccaattg agtaacctgt gagacttgaa acgtaagatg     6240

acttcacaca caaagtcact tgactcaacc gtggctctca attgcacaaa atcactctgc     6300

actaatctat tgcaggagtc aggctatgaa caactagacg acagctactt atgtgttata     6360

tagaggaata ttaaaaaatc taagaataat cataaagtta caaaataatt atcagatttc     6420

gagccacagg tcacccctaa catgtgttat tgcacaccca caatcctcag cttgatgtca     6480

tttaattctt ccagccacca tctctctctc caaccctaat ggcaaacttt attttggtgg     6540

agcgatgact cttactcaac tgcagcatac ttaagcacaa ttgttcccca gcctgatacg     6600

acacaccatc cattgtcaag cttcaccaca tacaacaaca cagcgtacgc aactaacatg     6660

aatgaatacg atatacatca aagactatga tacgcagtat tgcacactgt acgagtaaga     6720

gcactagcca ctgcactcaa gtgaaaccgt tgcccgggta cgagtatgag tatgtacagt     6780

atgtttagta ttgtacttgg acagtgcttg tatcgtacat tctcaagtgt caaacataaa     6840

tatccgttgc tatatcctcg caccaccacg tagctcgcta tatccctgtg ttgaatccat     6900

ccatcttgga ttgccaattg tgcacacaga accgggcact cacttcccca tccacacttg     6960

cggccgctta ggaatcctga gcgtccttga cacagtgaac cacaccgact ttgtgcatgt     7020

acttgagggt ggaaatgatg ttgcccacaa tggtagggta gaagacgtac cgaactccgt     7080

gtcgttcgca acactctcgg acagcttgct gcacgaaggg atagtgccaa gacgacattc     7140

gaggaaagag gtgatgctcg atctggaagt tgagaccgcc agtaaagaac atggcaatgg     7200

gtccaccgta ggtggaagag gtctccacct gagctctgta ccagtcgatc tgatcggctt     7260

caacgtcctt ctcggagctc ttgaccttgc agttcttgtc ggggattcgc tccgagccat     7320

cgaagttgtg agacaagatg aaaaagaagg tgaggaaggc accggtagca gtgggcacca     7380

gaggaatggt gatgagcagg gaggttccag tgagatacca gggcaagaag gcggttcgaa     7440

agatgaagaa agctcgcata acgaatgcaa gggttcggta ccgtcgcaga aagccgttct     7500

ctcgcatggc tgtgacagac tcgggaatgg tgtcgttgtg ctgcattcgg aagatgtaga     7560

gagggttgta caccagcgaa acgccgtagg ctccaagcac gaggtacatg taccaggcct     7620

ggaatcggtg aaaccacttt cgagcagtgt tggcagcagg gtagttgtgg aacacaagga     7680

atggttctgc ggactcggca tccaggtcga gaccatgctg attggtgtag gtgtgatgtc     7740

gcatgatgtg agactgcagc cagatccatc tggacgatcc aatgacgtcg atgccgtagg     7800

caaagagagc gttgacccag ggctttttgc tgatggcacc atgagaggca tcgtgctgaa     7860

tggacaggcc gatctgcatg tgcatgaatc cagtcaagag accccacagc accattccgg     7920

tagtagccca gtgccactcg caaaaggcgg tgacagcaat gatgccaacg gttcgcagcc     7980

agaatccagg tgtggcatac cagttccgac ctttcatgac ctctcgcata gttcgcttga     8040

cgtcctgtgc aaagggagag tcgtaggtgt agacaatgtc cttggaggtt cggtcgtgct     8100

tgcctcgcac gaactgttga agcagcttcg agttctcggg cttgacgtaa gggtgcatgg     8160

agtagaacag aggagaagca tcggaggcac cagaagcgag gatcaagtcg gatccgggat     8220

ggaccttggc aagaccttcc agatcgtaga gaatgccgtc gatggcaacc aggtcgggtc     8280

gctcgagcag ctgctcggta gtaagggaga gagccatgga gagctgggtt agtttgtgta     8340

gagagtgtgt gttgctagcg actttcggat tgtgtcatta cacaaaacgc gtcgtctcga     8400

cactgatctt gtcgtggata ctcacggctc ggacatcgtc gccgacgatg acaccggact     8460

ttcgcttaag gacgtcagta acaggcattg tgtgatgtgt agtttagatt tcgaatctgt     8520

ggggaaagaa aggaaaaaag agactggcaa ccgattggga gagccactgt ttatatatac     8580

cctagacaag ccccccgctt gtaagatgtt ggtcaatgta aaccagtatt aaggttggca     8640

agtgcaggag aagcaaggtg tgggtaccga gcaatggaaa tgtgcggaag gcaaaaaaat     8700

gaggccacgg cctattgtcg gggctatatc cagggggcga ttgaagtaca ctaacatgac     8760

atgtgtccac agaccctcaa tctggcctga tgagccaaat ccatacgcgc tttcgcagct     8820

ctaaaggcta taacaagtca caccaccctg ctcgacctca gcgccctcac tttttgttaa     8880

gacaaactgt acacgctgtt ccagcgtttt ctgcctgcac ctggtgggac atttggtgca     8940

acctaaagtg ctcggaacct ctgtggtgtc cagatcagcg cagcagttcc gaggtagttt     9000

tgaggccctt agatgatgca atggtgtcag tcgctggatc acgagtctta atggcagtat     9060

tcgttcttat ttgtgccatt gagccccgtt atcctcgtat cttctacccc ccatcccatc     9120

cctttgttgg tgcaacccta cccatttatt gttgggtgca gcccaaccga cgtggagagc     9180

ttggcttggc catataaaaa ggcccccccc tagtggcaat ggcagaaagt cagctgtgag     9240

ttgttgaatt tgtcatctag gcggcctggc cgtcttctcc ggggcaattg gggctgtttt     9300

ttgggacaca aatacgccgc caacccggtc tctcctgaat tctgcagatg ggctgcagga     9360

attccgtcgt cgcctgagtc gactccaact tttcacactg agcgtaaaat gtggagaaga     9420

aatcggcact aaaaagtcag gtagactgga aaatgcgcca tgaaatgaat atctcttgct     9480

acagtaatgc ccagcatcga ggggtattgt gtcaccaaca ctatagtggc agctgaagcg     9540

ctcgtgattg tagtatgagt ctttattggt gatgggaaga gttcactcaa tattctcgtt     9600

actgccaaaa caccacggta atcggccaga caccatggat gtagatcacc aagcctgtga     9660

atgttattcg agctaaaatg cacatggttg gtgaaaggag tagttgctgt cgaattccgt     9720

cgtcgcctga gtcatcattt atttaccagt tggccacaaa cccttgacga tctcgtatgt     9780

cccctccgac atactcccgg ccggctgggg tacgttcgat agcgctatcg gcatcgacaa     9840

ggtttgggtc cctagccgat accgcactac ctgagtcaca atcttcggag gtttagtctt     9900

ccacatagca cgggcaaaag tgcgtatata tacaagagcg tttgccagcc acagattttc     9960

actccacaca ccacatcaca catacaacca cacacatcca caatggaacc cgaaactaag    10020

aagaccaaga ctgactccaa gaagattgtt cttctcggcg gcgacttctg tggccccgag    10080

gtgattgccg aggccgtcaa ggtgctcaag tctgttgctg aggcctccgg caccgagttt    10140

gtgtttgagg accgactcat tggaggagct gccattgaga aggagggcga gcccatcacc    10200

gacgctactc tcgacatctg ccgaaaggct gactctatta tgctcggtgc tgtcggaggc    10260

gctgccaaca ccgtatggac cactcccgac ggacgaaccg acgtgcgacc cgagcagggt    10320

ctcctcaagc tgcgaaagga cctgaacctg tacgccaacc tgcgaccctg ccagctgctg    10380

tcgcccaagc tcgccgatct ctcccccatc cgaaacgttg agggcaccga cttcatcatt    10440

gtccgagagc tcgtcggagg tatctacttt ggagagcgaa aggaggatga cggatctggc    10500

gtcgcttccg acaccgagac ctactccgtt cctgaggttg agcgaattgc ccgaatggcc    10560

gccttcctgg cccttcagca caacccccct cttcccgtgt ggtctcttga caaggccaac    10620

gtgctggcct cctctcgact ttggcgaaag actgtcactc gagtcctcaa ggacgaattc    10680

ccccagctcg agctcaacca ccagctgatc gactcggccg ccatgatcct catcaagcag    10740

ccctccaaga tgaatggtat catcatcacc accaacatgt ttggcgatat catctccgac    10800

gaggcctccg tcatccccgg ttctctgggt ctgctgccct ccgcctctct ggcttctctg    10860

cccgacacca acgaggcgtt cggtctgtac gagccctgtc acggatctgc ccccgatctc    10920

ggcaagcaga aggtcaaccc cattgccacc attctgtctg ccgccatgat gctcaagttc    10980

tctcttaaca tgaagcccgc cggtgacgct gttgaggctg ccgtcaagga gtccgtcgag    11040

gctggtatca ctaccgccga tatcggaggc tcttcctcca cctccgaggt cggagacttg    11100

ttgccaacaa ggtcaaggag ctgctcaaga aggagtaagt cgtttctacg acgcattgat    11160

ggaaggagca aactgacgcg cctgcgggtt ggtctaccgg cagggtccgc tagtgtataa    11220

gactctataa aaagggccct gccctgctaa tgaaatgatg atttataatt taccggtgta    11280

gcaaccttga ctagaagaag cagattgggt gtgtttgtag tggaggacag tggtacgttt    11340

tggaaacagt cttcttgaaa gtgtcttgtc tacagtatat tcactcataa cctcaatagc    11400

caagggtgta gtcggtttat taaaggaagg gagttgtggc tgatgtggat agatatcttt    11460

aagctggcga ctgcacccaa cgagtgtggt ggtagcttgt ttaaacagag tgtgaaagac    11520

tcactatggt ccgggcttat ctcgaccaat agccaaagtc tggagtttct gagagaaaaa    11580

ggcaagatac gtatgtaaca aagcgacgca tggtacaata ataccggagg catgtatcat    11640

agagagttag tggttcgatg atggcactgg tgcctggtat gactttatac ggctgactac    11700

atatttgtcc tcagacatac aattacagtc aagcacttac ccttggacat ctgtaggtac    11760

cccccggcca agacgatctc agcgtgtcgt atgtcggatt ggcgtagctc cctcgctcgt    11820

caattggctc ccatctactt tcttctgctt ggctacaccc agcatgtctg ctatggctcg    11880

ttttcgtgcc ttatctatcc tcccagtatt accaactcta aatgacatga tgtgattggg    11940

tctacacttt catatcagag ataaggagta gcacagttgc ataaaaagcc caactctaat    12000

cagcttcttc ctttcttgta attagtacaa aggtgattag cgaaatctgg aagcttagtt    12060

ggccctaaaa aaatcaaaaa aagcaaaaaa cgaaaaacga aaaaccacag ttttgagaac    12120

agggaggtaa cgaaggatcg tatatatata tatatatata tatacccacg gatcccgaga    12180

ccggcctttg attcttccct acaaccaacc attctcacca ccctaattca caaccatggc    12240

caccatctcc ctgactaccg agcagctcct ggaacacccc gagctcgttg ccatcgacgg    12300

agtcctgtac gatctcttcg gtctggccaa ggtccatgcc ggaggcaacc tcatcgaagc    12360

tgccggtgca tccgacggaa ccgctctgtt ctactccatg catcctggag tcaagccaga    12420

gaactcgaag cttctgcagc aatttgcccg aggcaagcac gaacgaagct ccaaggatcc    12480

cgtgtacacc ttcgactctc cctttgctca ggacgtcaag cagtccgttc gagaggtcat    12540

gaagggtcga aactggtacg ccactcctgg cttctggctg agaaccgcac tcatcatcgc    12600

ttgtactgcc attggcgagt ggtactggat cacaaccgga gcagtgatgt ggggtatctt    12660

tactggatac ttccactcgc agattggctt ggccattcaa cacgatgctt ctcacggagc    12720

catcagcaaa aagccctggg tcaacgcctt tttcgcttat ggcatcgacg ccattggttc    12780

ctctcgttgg atctggctgc agtcccacat tatgcgacat cacacttaca ccaaccagca    12840

tggcctcgac ctggatgctg cctcggcaga gccgttcatc ttgttccact cctatcctgc    12900

taccaacgcc tctcgaaagt ggtaccaccg atttcaggcg tggtacatgt acatcgttct    12960

gggaatgtat ggtgtctcga tggtgtacaa tcccatgtac ctcttcacaa tgcagcacaa    13020

cgacaccatt cccgaggcca cttctctcag accaggcagc tttttcaatc ggcagcgagc    13080

tttcgccgtt tcccttcgac tgctcttcat cttccgaaac gcctttcttc cctggtacat    13140

tgctggtgcc tctcctctgc tcaccattct tctggtgccc acggtcacag gcatcttcct    13200

cacctttgtg ttcgttctgt cccataactt cgagggagcc gaacggaccc cagagaagaa    13260

ctgcaaggcc aaacgagcta aggaaggcaa ggaggtcaga gacgtggaag aggatcgagt    13320

cgactggtac cgagcacagg ccgagactgc tgccacctac ggtggcagcg tgggaatgat    13380

gcttacaggc ggtctcaacc tgcagatcga gcatcacttg tttccccgaa tgtcctcttg    13440

gcactatccc ttcattcaag acaccgttcg ggagtgttgc aagcgacatg gcgtccgtta    13500

cacatactat cctaccattc tcgagaacat catgtccact cttcgataca tgcagaaggt    13560

gggtgttgct cacaccattc aggatgccca ggagttctaa gcggccgcat gtacatacaa    13620

gattatttat agaaatgaat cgcgatcgaa caaagagtac gagtgtacga gtaggggatg    13680

atgataaaag tggaagaagt tccgcatctt tggatttatc aacgtgtagg acgatacttc    13740

ctgtaaaaat gcaatgtctt taccataggt tctgctgtag atgttattaa ctaccattaa    13800

catgtctact tgtacagttg cagaccagtt ggagtataga atggtacact taccaaaaag    13860

tgttgatggt tgtaactacg atatataaaa ctgttgacgg gatccccgct gatatgccta    13920

aggaacaatc aaagaggaag atattaattc agaatgctag tatacagtta gggat         13975


<210>  97
<211>  14619
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZP2-85m98F

<400>  97
cgatggaagc cggtagaacc gggctgcttg tgcttggaga tggaagccgg tagaaccggg       60

ctgcttgggg ggatttgggg ccgctgggct ccaaagaggg gtaggcattt cgttggggtt      120

acgtaattgc ggcatttggg tcctgcgcgc atgtcccatt ggtcagaatt agtccggata      180

ggagacttat cagccaatca cagcgccgga tccacctgta ggttgggttg ggtgggagca      240

cccctccaca gagtagagtc aaacagcagc agcaacatga tagttggggg tgtgcgtgtt      300

aaaggaaaaa aaagaagctt gggttatatt cccgctctat ttagaggttg cgggatagac      360

gccgacggag ggcaatggcg ctatggaacc ttgcggatat ccatacgccg cggcggactg      420

cgtccgaacc agctccagca gcgttttttc cgggccattg agccgactgc gaccccgcca      480

acgtgtcttg gcccacgcac tcatgtcatg ttggtgttgg gaggccactt tttaagtagc      540

acaaggcacc tagctcgcag caaggtgtcc gaaccaaaga agcggctgca gtggtgcaaa      600

cggggcggaa acggcgggaa aaagccacgg gggcacgaat tgaggcacgc cctcgaattt      660

gagacgagtc acggccccat tcgcccgcgc aatggctcgc caacgcccgg tcttttgcac      720

cacatcaggt taccccaagc caaacctttg tgttaaaaag cttaacatat tataccgaac      780

gtaggtttgg gcgggcttgc tccgtctgtc caaggcaaca tttatataag ggtctgcatc      840

gccggctcaa ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca      900

cacatcaacc atggtcaagc gacccgctct gcctctcacc gtggacggtg tcacctacga      960

cgtttctgcc tggctcaacc accatcccgg aggtgccgac attatcgaga actaccgagg     1020

tcgggatgct accgacgtct tcatggttat gcactccgag aacgccgtgt ccaaactcag     1080

acgaatgccc atcatggaac cttcctctcc cctgactcca acacctccca agccaaactc     1140

cgacgaacct caggaggatt tccgaaagct gcgagacgag ctcattgctg caggcatgtt     1200

cgatgcctct cccatgtggt acgcttacaa gaccctgtcg actctcggac tgggtgtcct     1260

tgccgtgctg ttgatgaccc agtggcactg gtacctggtt ggtgctatcg tcctcggcat     1320

tcactttcaa cagatgggat ggctctcgca cgacatttgc catcaccagc tgttcaagga     1380

ccgatccatc aacaatgcca ttggcctgct cttcggaaac gtgcttcagg gcttttctgt     1440

cacttggtgg aaggaccgac acaacgctca tcactccgcc accaacgtgc agggtcacga     1500

tcccgacatc gacaacctgc ctctcctggc gtggtccaag gaggacgtcg agcgagctgg     1560

cccgttttct cgacggatga tcaagtacca acagtattac ttctttttca tctgtgccct     1620

tctgcgattc atctggtgct ttcagtccat tcatactgcc acgggtctca aggatcgaag     1680

caatcagtac tatcgaagac agtacgagaa ggagtccgtc ggtctggcac tccactgggg     1740

tctcaaggcc ttgttctact atttctacat gccctcgttt ctcaccggac tcatggtgtt     1800

ctttgtctcc gagctgcttg gtggcttcgg aattgccatc gttgtcttca tgaaccacta     1860

ccctctggag aagattcagg actccgtgtg ggatggtcat ggcttctgtg ctggacagat     1920

tcacgagacc atgaacgttc agcgaggcct cgtcacagac tggtttttcg gtggcctcaa     1980

ctaccagatc gaacatcacc tgtggcctac tcttcccaga cacaacctca ccgctgcctc     2040

catcaaagtg gagcagctgt gcaagaagca caacctgccc taccgatctc ctcccatgct     2100

cgaaggtgtc ggcattctta tctcctacct gggcaccttc gctcgaatgg ttgccaaggc     2160

agacaaggcc taagcggccg cattgatgat tggaaacaca cacatgggtt atatctaggt     2220

gagagttagt tggacagtta tatattaaat cagctatgcc aacggtaact tcattcatgt     2280

caacgaggaa ccagtgactg caagtaatat agaatttgac caccttgcca ttctcttgca     2340

ctcctttact atatctcatt tatttcttat atacaaatca cttcttcttc ccagcatcga     2400

gctcggaaac ctcatgagca ataacatcgt ggatctcgtc aatagagggc tttttggact     2460

ccttgctgtt ggccaccttg tccttgctgt ttaaacatcg tggttaatgc tgctgtgtgc     2520

tgtgtgtgtg tgttgtttgg cgctcattgt tgcgttatgc agcgtacacc acaatattgg     2580

aagcttatta gcctttctat tttttcgttt gcaaggctta acaacattgc tgtggagagg     2640

gatggggata tggaggccgc tggagggagt cggagaggcg ttttggagcg gcttggcctg     2700

gcgcccagct cgcgaaacgc acctaggacc ctttggcacg ccgaaatgtg ccacttttca     2760

gtctagtaac gccttaccta cgtcattcca tgcgtgcatg tttgcgcctt ttttcccttg     2820

cccttgatcg ccacacagta cagtgcactg tacagtggag gttttggggg ggtcttagat     2880

gggagctaaa agcggcctag cggtacacta gtgggattgt atggagtggc atggagccta     2940

ggtggagcct gacaggacgc acgaccggct agcccgtgac agacgatggg tggctcctgt     3000

tgtccaccgc gtacaaatgt ttgggccaaa gtcttgtcag ccttgcttgc gaacctaatt     3060

cccaattttg tcacttcgca cccccattga tcgagcccta acccctgccc atcaggcaat     3120

ccaattaagc tcgcattgtc tgccttgttt agtttggctc ctgcccgttt cggcgtccac     3180

ttgcacaaac acaaacaagc attatatata aggctcgtct ctccctccca accacactca     3240

cttttttgcc cgtcttccct tgctaacaca aaagtcaaga acacaaacaa ccaccccaac     3300

ccccttacac acaagacata tctacagcaa tggccatggc tctctccctt actaccgagc     3360

agctgctcga gcgacccgac ctggttgcca tcgacggcat tctctacgat ctggaaggtc     3420

ttgccaaggt ccatcccgga tccgacttga tcctcgcttc tggtgcctcc gatgcttctc     3480

ctctgttcta ctccatgcac ccttacgtca agcccgagaa ctcgaagctg cttcaacagt     3540

tcgtgcgagg caagcacgac cgaacctcca aggacattgt ctacacctac gactctccct     3600

ttgcacagga cgtcaagcga actatgcgag aggtcatgaa aggtcggaac tggtatgcca     3660

cacctggatt ctggctgcga accgttggca tcattgctgt caccgccttt tgcgagtggc     3720

actgggctac taccggaatg gtgctgtggg gtctcttgac tggattcatg cacatgcaga     3780

tcggcctgtc cattcagcac gatgcctctc atggtgccat cagcaaaaag ccctgggtca     3840

acgctctctt tgcctacggc atcgacgtca ttggatcgtc cagatggatc tggctgcagt     3900

ctcacatcat gcgacatcac acctacacca atcagcatgg tctcgacctg gatgccgagt     3960

ccgcagaacc attccttgtg ttccacaact accctgctgc caacactgct cgaaagtggt     4020

ttcaccgatt ccaggcctgg tacatgtacc tcgtgcttgg agcctacggc gtttcgctgg     4080

tgtacaaccc tctctacatc ttccgaatgc agcacaacga caccattccc gagtctgtca     4140

cagccatgcg agagaacggc tttctgcgac ggtaccgaac ccttgcattc gttatgcgag     4200

ctttcttcat ctttcgaacc gccttcttgc cctggtatct cactggaacc tccctgctca     4260

tcaccattcc tctggtgccc actgctaccg gtgccttcct caccttcttt ttcatcttgt     4320

ctcacaactt cgatggctcg gagcgaatcc ccgacaagaa ctgcaaggtc aagagctccg     4380

agaaggacgt tgaagccgat cagatcgact ggtacagagc tcaggtggag acctcttcca     4440

cctacggtgg acccattgcc atgttcttta ctggcggtct caacttccag atcgagcatc     4500

acctctttcc tcgaatgtcg tcttggcact atcccttcgt gcagcaagct gtccgagagt     4560

gttgcgaacg acacggagtt cggtacgtct tctaccctac cattgtgggc aacatcattt     4620

ccaccctcaa gtacatgcac aaagtcggtg tggttcactg tgtcaaggac gctcaggatt     4680

cctaagcggc cgcatgagaa gataaatata taaatacatt gagatattaa atgcgctaga     4740

ttagagagcc tcatactgct cggagagaag ccaagacgag tactcaaagg ggattacacc     4800

atccatatcc acagacacaa gctggggaaa ggttctatat acactttccg gaataccgta     4860

gtttccgatg ttatcaatgg gggcagccag gatttcaggc acttcggtgt ctcggggtga     4920

aatggcgttc ttggcctcca tcaagtcgta ccatgtcttc atttgcctgt caaagtaaaa     4980

cagaagcaga tgaagaatga acttgaagtg aaggaattta aatgtaacga aactgaaatt     5040

tgaccagata ttgtgtccgc ggtggagctc cagcttttgt tccctttagt gagggttaat     5100

ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac     5160

aagcttccac acaacgtacg ggcgtcgttg cttgtgtgat ttttgaggac ccatcccttt     5220

ggtatataag tatactctgg ggttaaggtt gcccgtgtag tctaggttat agttttcatg     5280

tgaaataccg agagccgagg gagaataaac gggggtattt ggacttgttt ttttcgcgga     5340

aaagcgtcga atcaaccctg cgggccttgc accatgtcca cgacgtgttt ctcgccccaa     5400

ttcgcccctt gcacgtcaaa attaggcctc catctagacc cctccataac atgtgactgt     5460

ggggaaaagt ataagggaaa ccatgcaacc atagacgacg tgaaagacgg ggaggaacca     5520

atggaggcca aagaaatggg gtagcaacag tccaggagac agacaaggag acaaggagag     5580

ggcgcccgaa agatcggaaa aacaaacatg tccaattggg gcagtgacgg aaacgacacg     5640

gacacttcag tacaatggac cgaccatctc caagccaggg ttattccggt atcaccttgg     5700

ccgtaacctc ccgctggtac ctgatattgt acacgttcac attcaatata ctttcagcta     5760

caataagaga ggctgtttgt cgggcatgtg tgtccgtcgt atggggtgat gtccgagggc     5820

gaaattcgct acaagcttaa ctctggcgct tgtccagtat gaatagacaa gtcaagacca     5880

gtggtgccat gattgacagg gaggtacaag acttcgatac tcgagcatta ctcggacttg     5940

tggcgattga acagacgggc gatcgcttct cccccgtatt gccggcgcgc cagctgcatt     6000

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct     6060

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     6120

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     6180

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     6240

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     6300

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     6360

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     6420

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     6480

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     6540

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     6600

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     6660

acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     6720

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     6780

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     6840

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     6900

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     6960

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     7020

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta     7080

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     7140

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     7200

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     7260

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt     7320

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     7380

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     7440

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     7500

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     7560

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg     7620

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     7680

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     7740

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     7800

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     7860

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     7920

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     7980

atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa     8040

gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc     8100

aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga     8160

gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag     8220

ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt     8280

ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta     8340

gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag     8400

cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg     8460

cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga     8520

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     8580

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     8640

cagtgaattg taatacgact cactataggg cgaattgggc ccgacgtcgc atgcgctgat     8700

gacactttgg tctgaaagag atgcattttg aatcccaaac ttgcagtgcc caagtgacat     8760

acatctccgc gttttggaaa atgttcagaa acagttgatt gtgttggaat ggggaatggg     8820

gaatggaaaa atgactcaag tatcaattcc aaaaacttct ctggctggca gtacctactg     8880

tccatactac tgcattttct ccagtcaggc cactctatac tcgacgacac agtagtaaaa     8940

cccagataat ttcgacataa acaagaaaac agacccaata atatttatat atagtcagcc     9000

gtttgtccag ttcagactgt aatagccgaa aaaaaatcca aagtttctat tctaggaaaa     9060

tatattccaa tatttttaat tcttaatctc atttatttta ttctagcgaa atacatttca     9120

gctacttgag acatgtgata cccacaaatc ggattcggac tcggttgttc agaagagcat     9180

atggcattcg tgctcgcttg ttcacgtatt cttcctgttc catctcttgg ccgacaatca     9240

cacaaaaatg gggttttttt tttaattcta atgattcatt acagcaaaat tgagatatag     9300

cagaccacgt attccataat caccaaggaa gttcttgggc gtcttaatta actcacctgc     9360

aggattgaga ctatgaatgg attcccgtgc ccgtattact ctactaattt gatcttggaa     9420

cgcgaaaata cgtttctagg actccaaaga atctcaactc ttgtccttac taaatatact     9480

acccatagtt gatggtttac ttgaacagag aggacatgtt cacttgaccc aaagtttctc     9540

gcatctcttg gatatttgaa caacggcgtc cactgaccgt cagttatcca gtcacaaaac     9600

ccccacattc atacattccc atgtacgttt acaaagttct caattccatc gtgcaaatca     9660

aaatcacatc tattcattca tcatatataa acccatcatg tctactaaca ctcacaactc     9720

catagaaaac atcgactcag aacacacgct ccatgcggcc gcttactgag ccttggcacc     9780

gggctgcttc tcggccattc gagcgaactg ggacaggtat cggagcagga tgacgagacc     9840

ttcatggggc agagggtttc ggtaggggag gttgtgcttc tggcacagct gttccacctg     9900

gtaggaaacg gcagtgaggt tgtgtcgagg cagggtgggc cagagatggt gctcgatctg     9960

gtagttcagg cctccaaaga accagtcagt aatgatgcct cgtcgaatgt tcatggtctc    10020

atggatctga cccacagaga agccatgtcc gtcccagacg gaatcaccga tcttctccag    10080

agggtagtgg ttcatgaaga ccacgatggc aattccgaag ccaccgacga gctcggaaac    10140

aaagaacacc agcatcgagg tcaggatgga gggcataaag aagaggtgga acagggtctt    10200

gagagtccag tgcagagcga gtccaatggc ctctttcttg tactgagatc ggtagaactg    10260

gttgtctcgg tccttgaggg atcgaacggt cagcacagac tggaaacacc agatgaatcg    10320

caggagaata cagatgacca ggaaatagta ctgttggaac tgaatgagct ttcgggagat    10380

gggagaagct cgagtgacat cgtcctcgga ccaggcgagc agaggcaggt tatcaatgtc    10440

gggatcgtga ccctgaacgt tggtagcaga atgatgggcg ttgtgtctgt ccttccacca    10500

ggtcacggag aagccctgga gtccgttgcc aaagaccaga cccaggacgt tattccagtt    10560

tcggttcttg aaggtctggt ggtggcagat gtcatgagac agccatccca tttgctggta    10620

gtgcataccg agcacgagag caccaatgaa gtacaggtgg tactggacca gcatgaagaa    10680

ggcaagcacg ccaagaccca gggtggtcaa gatcttgtac gagtaccaga ggggagaggc    10740

gtcaaacatg ccagtggcga tcagctcttc tcggagcttt cggaaatcct cctgagcttc    10800

gttgacggca gcctggggag gcagctcgga agcctggttg atcttgggca ttcgcttgag    10860

cttgtcgaag gcttcctgag agtgcataac catgaaggcg tcagtagcat ctcgtccctg    10920

gtagttctca atgatttcag ctccaccagg gtggaagttc acccaagcgg agacgtcgta    10980

cacctttccg tcgatgacga ggggcagagc ctgtcgagaa gccttcacgg atcccatgac    11040

ggccagagag tcgtagtagg tagcgggagg aagtccggca ggtcgagcgg gaccggcgcc    11100

ctgaatcttt ttggctccct tgtgctttcg gacgatgtag gtctgcacgt agaagttgag    11160

gaacagacac aggacagtac caacgtagaa gtagttgaaa aaccagccaa acattctcat    11220

tccatcttgt cggtagcagg gaatgttccg gtacttccag acgatgtaga agccaacgtt    11280

gaactgaatg atctgcatag aagtaatcag ggacttgggc atagggaact tgagcttgat    11340

cagtcgggtc caatagtagc cgtacatgat ccagtgaatg aagccgttga gcagcacaaa    11400

gatccaaacg gcttcgtttc ggtagttgta gaacagccac atgtccatag gagctccgag    11460

atggtgaaag aactgcaacc aggtcagagg cttgcccatg aggggcagat agaaggagtc    11520

aatgtactcg aggaacttgc tgaggtagaa cagctgagtg gtgattcgga agacattgtt    11580

gtcgaaagcc ttctcgcagt tgtcggacat gacaccaatg gtgtacatgg cgtaggccat    11640

agagaggaag gagcccagcg agtagatgga catgagcagg ttgtagttgg tgaacacaaa    11700

cttcattcga gactgaccct tgggtccgag aggaccaagg gtgaacttca ggatgacgaa    11760

ggcgatggag aggtacagca cctcgcagtg cgaggcatca gaccagagct gagcatagtc    11820

gaccttggga agaacctcct ggccaatgga gacgatttcg ttcacgacct ccatggttgt    11880

gaattagggt ggtgagaatg gttggttgta gggaagaatc aaaggccggt ctcgggatcc    11940

gtgggtatat atatatatat atatatatac gatccttcgt tacctccctg ttctcaaaac    12000

tgtggttttt cgtttttcgt tttttgcttt ttttgatttt tttagggcca actaagcttc    12060

cagatttcgc taatcacctt tgtactaatt acaagaaagg aagaagctga ttagagttgg    12120

gctttttatg caactgtgct actccttatc tctgatatga aagtgtagac ccaatcacat    12180

catgtcattt agagttggta atactgggag gatagataag gcacgaaaac gagccatagc    12240

agacatgctg ggtgtagcca agcagaagaa agtagatggg agccaattga cgagcgaggg    12300

agctacgcca atccgacata cgacacgctg agatcgtctt ggccgggggg tacctacaga    12360

tgtccaaggg taagtgcttg actgtaattg tatgtctgag gacaaatatg tagtcagccg    12420

tataaagtca taccaggcac cagtgccatc atcgaaccac taactctcta tgatacatgc    12480

ctccggtatt attgtaccat gcgtcgcttt gttacatacg tatcttgcct ttttctctca    12540

gaaactccag aattctctct cttgagcttt tccataacaa gttcttctgc ctccaggaag    12600

tccatgggtg gtttgatcat ggttttggtg tagtggtagt gcagtggtgg tattgtgact    12660

ggggatgtag ttgagaataa gtcatacaca agtcagcttt cttcgagcct catataagta    12720

taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa cacaacaaca    12780

tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca tactcgatca    12840

gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca cgctctctat    12900

atacacagtt aaattacata tccatagtct aacctctaac agttaatctt ctggtaagcc    12960

tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt ctggccgtac    13020

agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc aacagttcgg    13080

tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt cagaataagc    13140

cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac aaactcgggg    13200

tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag agagcccttg    13260

caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg agaggggact    13320

aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt ctgttcagag    13380

acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg tacaccgtgg    13440

gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg cttgacagtg    13500

ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt aagagcaagt    13560

tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc gatatgggtt    13620

ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc cttggtggtg    13680

gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt gagcactcga    13740

gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat tttggtggtg    13800

aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt atctggggca    13860

gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata gactggacta    13920

tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc gcctttgccg    13980

acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt gttgtcggcc    14040

aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg tatcgtcaaa    14100

gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga cgagtcagac    14160

agatactcgt cgaccttttc cttgggaacc accaccgtca gcccttctga ctcacgtatt    14220

gtagccaccg acacaggcaa cagtccgtgg atagcagaat atgtcttgtc ggtccatttc    14280

tcaccaactt taggcgtcaa gtgaatgttg cagaagaagt atgtgccttc attgagaatc    14340

ggtgttgctg atttcaataa agtcttgaga tcagtttggc cagtcatgtt gtggggggta    14400

attggattga gttatcgcct acagtctgta caggtatact cgctgcccac tttatacttt    14460

ttgattccgc tgcacttgaa gcaatgtcgt ttaccaaaag tgagaatgct ccacagaaca    14520

caccccaggg tatggttgag caaaaaataa acactccgat acggggaatc gaaccccggt    14580

ctccacggtt ctcaagaagt attcttgatg agagcgtat                           14619


<210>  98
<211>  993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  993 bp stuffer fragment

<400>  98
ctctatccaa tcacacagtc aactccgact accacgctcc ggcgggcagt ggagccttct       60

actctcacca gaatggctct gagcgcgaca tgccaccgcc cgccacgtcc taccgaggat      120

accagcctcc gggatattga aaaaaaagtg taaatattta ttatcgtcaa tcgatcacga      180

attatctgaa cggaatgaat gcagccgtgt gtgagctgtg tagatcatgt aacgtaaacg      240

gatacaatgg ctccatcctt tcttgactcg tttccacttc tgcaattgag tcgtaggagg      300

tgatctcact catgatcatg acttgtgcac taacgttggc tctacagaat agtcagaata      360

gtccagctcg tgccagtcgc gtgatttgaa acgcgaccaa caatgagaat gtttcataag      420

ctgcgctaag ttggtgatgt ctcacattca cattcgtttc atgacgcgat caaacttgtc      480

tacagttacc tattgcgaaa caaaactgcg ggacagtttc ttctatcatc tataaatcat      540

taatttatac aatccgtatc cgacggattc gtttttccca ttctttttgt ctgcgtctcc      600

gtctaagcga gagaagtgaa gaacttgtgg gcgagctcga gcggccgcat tctagatgca      660

gacgcacctt gaccagatca aacggatgac caaccagaac ggcgcagata cctccaaagc      720

caccggcggc gaaggactcg gcctgcgaca ggaacgactt gagagcggaa gggggagcga      780

tggccttaac gccagagtca atttcgggag cgtcactcat tgtgtggggt gtgtggaagg      840

gatagttgag tgcagttagg agatggcaac gtccgagtta taagaagtgt ttagagttgt      900

tcacacggga aggttacctc ggcatgtgaa tatggaggat tcggtatagg ggggtgtagc      960

ggcgggttta gggggcgatt gtgtatatat atg                                   993


<210>  99
<211>  7338
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pYPS234

<400>  99
aaatgagtat ctgtctgact cgtcattgcc gcctttggag tacgactcca actatgagtg       60

tgcttggatc actttgacga tacattcttc gttggaggct gtgggtctga cagctgcgtt      120

ttcggcgcgg ttggccgaca acaatatcag ctgcaacgtc attgctggct ttcatcatga      180

tcacattttt gtcggcaaag gcgacgccca gagagccatt gacgttcttt ctaatttgga      240

ccgatagccg tatagtccag tctatctata agttcaacta actcgtaact attaccataa      300

catatacttc actgccccag ataaggttcc gataaaaagt tctgcagact aaatttattt      360

cagtctcctc ttcaccacca aaatgccctc ctacgaagct cgagctaacg tccacaagtc      420

cgcctttgcc gctcgagtgc tcaagctcgt ggcagccaag aaaaccaacc tgtgtgcttc      480

tctggatgtt accaccacca aggagctcat tgagcttgcc gataaggtcg gaccttatgt      540

gtgcatgatc aaaacccata tcgacatcat tgacgacttc acctacgccg gcactgtgct      600

ccccctcaag gaacttgctc ttaagcacgg tttcttcctg ttcgaggaca gaaagttcgc      660

agatattggc aacactgtca agcaccagta ccggtgtcac cgaatcgccg agtggtccga      720

tatcaccaac gcccacggtg tacccggaac cggaatcatt gctggcctgc gagctggtgc      780

cgaggaaact gtctctgaac agaagaagga ggacgtctct gactacgaga actcccagta      840

caaggagttc ctagtcccct ctcccaacga gaagctggcc agaggtctgc tcatgctggc      900

cgagctgtct tgcaagggct ctctggccac tggcgagtac tccaagcaga ccattgagct      960

tgcccgatcc gaccccgagt ttgtggttgg cttcattgcc cagaaccgac ctaagggcga     1020

ctctgaggac tggcttattc tgacccccgg ggtgggtctt gacgacaagg gagacgctct     1080

cggacagcag taccgaactg ttgaggatgt catgtctacc ggaacggata tcataattgt     1140

cggccgaggt ctgtacggcc agaaccgaga tcctattgag gaggccaagc gataccagaa     1200

ggctggctgg gaggcttacc agaagattaa ctgttagagg ttagactatg gatatgtaat     1260

ttaactgtgt atatagagag cgtgcaagta tggagcgctt gttcagcttg tatgatggtc     1320

agacgacctg tctgatcgag tatgtatgat actgcacaac ctgtgtatcc gcatgatctg     1380

tccaatgggg catgttgttg tgtttctcga tacggagatg ctgggtacag tgctaatacg     1440

ttgaactact tatacttata tgaggctcga agaaagctga cttgtgtatg acttaattaa     1500

cgcggcgcgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     1560

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     1620

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     1680

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     1740

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     1800

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     1860

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     1920

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt     1980

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     2040

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     2100

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     2160

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa     2220

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     2280

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     2340

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     2400

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     2460

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt     2520

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact     2580

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat     2640

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg     2700

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg     2760

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat     2820

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc     2880

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt     2940

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc     3000

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga     3060

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc     3120

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa     3180

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta     3240

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg     3300

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg     3360

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat     3420

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt     3480

tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat     3540

accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta     3600

aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga     3660

atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa     3720

cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga     3780

accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc     3840

taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga     3900

agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg     3960

cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc     4020

aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg     4080

gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca     4140

cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggc     4200

ccgacgtcgc atgcatcaat cgatgactgt cgactcacgt acgggtggtg agaatggttg     4260

gttgtaggga agaatcaaag gccggtctcg ggatccgtgg gtatatatat atatatatat     4320

atatacgatc cttcgttacc tccctgttct caaaactgtg gtttttcgtt tttcgttttt     4380

tgcttttttt gattttttta gggccaacta agcttccaga tttcgctaat cacctttgta     4440

ctaattacaa gaaaggaaga agctgattag agttgggctt tttatgcaac tgtgctactc     4500

cttatctctg atatgaaagt gtagacccaa tcacatcatg tcatttagag ttggtaatac     4560

tgggaggata gataaggcac gaaaacgagc catagcagac atgctgggtg tagccaagca     4620

gaagaaagta gatgggagcc aattgacgag cgagggagct acgccaatcc gacatacgac     4680

acgctgagat cgtcttggcc ggggggtacc tacagatgtc caagggtaag tgcttgactg     4740

taattgtatg tctgaggaca aatatgtagt cagccgtata aagtcatacc aggcaccagt     4800

gccatcatcg aaccactaac tctctatgat acatgcctcc ggtattattg taccatgcgt     4860

cgctttgtta catacgtatc ttgccttttt ctctcagaaa ctccagactt tggctattgg     4920

tcgagataag cccggaccat agtgagtctt tcacactctg tttaaacaag ctaccaccac     4980

actcgttggg tgcagtcgcc agcttaaaga tatctatcca catcagccac aactcccttc     5040

ctttaataaa ccgactacac ccttggctat tgaggttatg agtgaatata ctgtagacaa     5100

gacactttca agaagactgt ttccaaaacg taccactgtc ctccactaca aacacaccca     5160

atctgcttct tctagtcaag gttgctacac cggtaaatta taaatcatca tttcattagc     5220

agggcagggc cctttttata gagtcttata cactagcgga ccctgccggt agaccaaccc     5280

gcaggcgcgt cagtttgctc cttccatcaa tgcgtcgtag actagtctct atccaatcac     5340

acagtcaact ccgactacca cgctccggcg ggcagtggag ccttctactc tcaccagaat     5400

ggctctgagc gcgacatgcc accgcccgcc acgtcctacc gaggatacca gcctccggga     5460

tattgaaaaa aaagtgtaaa tatttattat cgtcaatcga tcacgaatta tctgaacgga     5520

atgaatgcag ccgtgtgtga gctgtgtaga tcatgtaacg taaacggata caatggctcc     5580

atcctttctt gactcgtttc cacttctgca attgagtcgt aggaggtgat ctcactcatg     5640

atcatgactt gtgcactaac gttggctcta cagaatagtc agaatagtcc agctcgtgcc     5700

agtcgcgtga tttgaaacgc gaccaacaat gagaatgttt cataagctgc gctaagttgg     5760

tgatgtctca cattcacatt cgtttcatga cgcgatcaaa cttgtctaca gttacctatt     5820

gcgaaacaaa actgcgggac agtttcttct atcatctata aatcattaat ttatacaatc     5880

cgtatccgac ggattcgttt ttcccattct ttttgtctgc gtctccgtct aagcgagaga     5940

agtgaagaac ttgtgggcga gctcgagcgg ccgcattcta gatgcagacg caccttgacc     6000

agatcaaacg gatgaccaac cagaacggcg cagatacctc caaagccacc ggcggcgaag     6060

gactcggcct gcgacaggaa cgacttgaga gcggaagggg gagcgatggc cttaacgcca     6120

gagtcaattt cgggagcgtc actcattgtg tggggtgtgt ggaagggata gttgagtgca     6180

gttaggagat ggcaacgtcc gagttataag aagtgtttag agttgttcac acgggaaggt     6240

tacctcggca tgtgaatatg gaggattcgg tatagggggg tgtagcggcg ggtttagggg     6300

gcgattgtgt atatatatgg gatcccggtt ctgtgtgcac aattggcaat ccaagatgga     6360

tggattcaac acagggatat agcgagctac gtggtggtgc gaggatatag caacggatat     6420

ttatgtttga cacttgagaa tgtacgatac aagcactgtc caagtacaat actaaacata     6480

ctgtacatac tcatactcgt acccgggcaa cggtttcact tgagtgcagt ggctagtgct     6540

cttactcgta cagtgtgcaa tactgcgtat catagtcttt gatgtatatc gtattcattc     6600

atgttagttg cgtacgctgt gttgttgtat gtggtgaagc ttgacaatgg atggtgtgtc     6660

gtatcaggct ggggaacaat tgtgcttaag tatgctgcag ttgagtaaga gtcatcgctc     6720

caccaaaata aagtttgcca ttagggttgg agagagagat ggtggctgga agaattaaat     6780

gacatcaagc tgaggattgt gggtgtgcaa taacacatgt taggggtgac ctgtggctcg     6840

aaatctgata attattttgt aactttatga ttattcttag attttttaat attcctctat     6900

ataacacata agtagctgtc gtctagttgt tcatagcctg actcctgcaa tagattagtg     6960

cagagtgatt ttgtgcaatt gagagccacg gttgagtcaa gtgactttgt gtgtgaagtc     7020

atcttacgtt tcaagtctca caggttactc aattggttgg ttgtctgccc tttacagata     7080

tttacagtac ctgagcgtaa agtcgttcat ccacggaatg actgttcctg tcacgcagtc     7140

atgatcatgg atgtggctgg tcaggaacca ttttggatag gagacttagg gattggacta     7200

ttattgaaaa aactgagccg aatatgatat agttctattt gaatgcagaa cttctgatgg     7260

tcaattcact tatttcaggc atatcggtca tggtggcagc tgccacgatg ttatctcgtt     7320

ggaaaagatc ttcgattt                                                   7338


<210>  100
<211>  1019
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1019 bp stuffer fragment

<400>  100
actcactctg tcagctgccc cacattgccc aatgcacaat gcacaatgat gtgtgcaaac       60

aacgcaatca aaagtctatg catgctgacc aaactctgat caccaagttg cgaacatgaa      120

aaagaagacc tgtgtatata taagtaaggg ggagagccct aactagatct ttcgaaaacc      180

ccccgacctt caccttccac aaccatgatc atcttatacg ttttggccgt tgcggtctcc      240

ttcctcatct tcaagagagt cacctacacg atgcgaagcc gagagctcgc caagaagtgg      300

cactgtgagg agcctcacaa cctgaatgat ctagatagcg gccgccatct taacggtgtg      360

ttcgttaaga tgacccggga cgaggctgcc tttgccgaga ccgagaagct cattaacgca      420

taaagggatg tatacttgta ctgtagatgg attaataaat tatttatgat gaacaaacat      480

tgactgagaa aggcaccata ttgtagcttc aagtactgta ctggtagaag taagcctgtc      540

gttacccact tatggagatc caatattgtt tgatgcggcc atcggactgc ttggagcgta      600

cagagaattg gtaaaatgtg gtggctgggt tggtttcgat tgaaattatc aagatctctg      660

aggtattctt ggtgttgaag ggatgttctt ggactactgc ttgtaacata gatgggatgt      720

agggatggca tggggtataa tcgagacaac cattgtggag gtaggcaaga gctcttacaa      780

gtaccatacg agtacaacta ctgaggcata atgtctggaa ttcccggtta agtccattac      840

agttgatatc attcaaatcg gacagatatt ctccgaggtg atacatctct gtccccgaga      900

gaagctgatt tggagcacat ccaggttacg gggggagaga aatttatgta atgtgaggat      960

tttttggagc tgaataataa agtcctgttg atctagcttc aattgagtca acatccgcc      1019


<210>  101
<211>  7364
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pYPS233

<400>  101
aaatgagtat ctgtctgact cgtcattgcc gcctttggag tacgactcca actatgagtg       60

tgcttggatc actttgacga tacattcttc gttggaggct gtgggtctga cagctgcgtt      120

ttcggcgcgg ttggccgaca acaatatcag ctgcaacgtc attgctggct ttcatcatga      180

tcacattttt gtcggcaaag gcgacgccca gagagccatt gacgttcttt ctaatttgga      240

ccgatagccg tatagtccag tctatctata agttcaacta actcgtaact attaccataa      300

catatacttc actgccccag ataaggttcc gataaaaagt tctgcagact aaatttattt      360

cagtctcctc ttcaccacca aaatgccctc ctacgaagct cgagctaacg tccacaagtc      420

cgcctttgcc gctcgagtgc tcaagctcgt ggcagccaag aaaaccaacc tgtgtgcttc      480

tctggatgtt accaccacca aggagctcat tgagcttgcc gataaggtcg gaccttatgt      540

gtgcatgatc aaaacccata tcgacatcat tgacgacttc acctacgccg gcactgtgct      600

ccccctcaag gaacttgctc ttaagcacgg tttcttcctg ttcgaggaca gaaagttcgc      660

agatattggc aacactgtca agcaccagta ccggtgtcac cgaatcgccg agtggtccga      720

tatcaccaac gcccacggtg tacccggaac cggaatcatt gctggcctgc gagctggtgc      780

cgaggaaact gtctctgaac agaagaagga ggacgtctct gactacgaga actcccagta      840

caaggagttc ctagtcccct ctcccaacga gaagctggcc agaggtctgc tcatgctggc      900

cgagctgtct tgcaagggct ctctggccac tggcgagtac tccaagcaga ccattgagct      960

tgcccgatcc gaccccgagt ttgtggttgg cttcattgcc cagaaccgac ctaagggcga     1020

ctctgaggac tggcttattc tgacccccgg ggtgggtctt gacgacaagg gagacgctct     1080

cggacagcag taccgaactg ttgaggatgt catgtctacc ggaacggata tcataattgt     1140

cggccgaggt ctgtacggcc agaaccgaga tcctattgag gaggccaagc gataccagaa     1200

ggctggctgg gaggcttacc agaagattaa ctgttagagg ttagactatg gatatgtaat     1260

ttaactgtgt atatagagag cgtgcaagta tggagcgctt gttcagcttg tatgatggtc     1320

agacgacctg tctgatcgag tatgtatgat actgcacaac ctgtgtatcc gcatgatctg     1380

tccaatgggg catgttgttg tgtttctcga tacggagatg ctgggtacag tgctaatacg     1440

ttgaactact tatacttata tgaggctcga agaaagctga cttgtgtatg acttaattaa     1500

cgcggcgcgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     1560

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     1620

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     1680

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     1740

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     1800

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     1860

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     1920

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt     1980

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     2040

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     2100

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     2160

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa     2220

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     2280

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     2340

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     2400

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     2460

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt     2520

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact     2580

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat     2640

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg     2700

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg     2760

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat     2820

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc     2880

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt     2940

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc     3000

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga     3060

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc     3120

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa     3180

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta     3240

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg     3300

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg     3360

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat     3420

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt     3480

tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat     3540

accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta     3600

aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga     3660

atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa     3720

cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga     3780

accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc     3840

taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga     3900

agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg     3960

cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc     4020

aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg     4080

gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca     4140

cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggc     4200

ccgacgtcgc atgcattcca tagccacacc tttgcctatg gcttcacaac cgaaggcaat     4260

tcgagaggtc gcgcttatgg aatcgactcg tataaagctg aagggaaagg gagacgttcc     4320

gagcgctcag atgcaatagt cgtccagcta atgtggattc aaaaacaacc ccaacagtaa     4380

tcttgaaaat ttgaacggat caatctgaac actcttgctc caggtcattc ttctaacgca     4440

catccccaga gtctagaggg agttgtgttg tgaacatcct aataaacaat gcaatggatt     4500

cgggatatct tctgtctcgc cccctactcg atgtcgagta aaccgatcac caactaacaa     4560

tactcctccg cgttctgcca ttgactctca aacagacatc gctatcaacg gaacagcata     4620

ttttagcttc ttaggacaat aaatattgat aatgccggct ctccctcggt atattaagca     4680

atccattcat acactcattc atcaggttaa ttttatatat ataatttgtc tattcaaaca     4740

ccgtaaatta ctggtaccat catctcctcc ttttcaaata cacgtctatt tgcattaatg     4800

aaattactcg ccaattcgca gaacgtgttt gtcgaacaga gccttagctc gggtccagac     4860

aggagcagtg tctcgctgag gaagctgcag gagagttaat taaaaggcgt tgaaacagaa     4920

tgagccagac agcaaggaca aggtggccaa cagcaaggag tccaaaaagc cctctattga     4980

cgagatccac gatgttattg ctcatgaggt ttccgagctc gatgctggga agaagaagtg     5040

atttgtatat aagaaataaa tgagatatag taaaggagtg caagagaatg gcaaggtggt     5100

caaattctat attacttgca gtcactggtt cctcgttgac atgaatgaag ttaccgttgg     5160

catagctgat ttaatatata actgtccaac taactctcac ctagatataa cccatgtgtg     5220

tgtttccacg cgtactcact ctgtcagctg ccccacattg cccaatgcac aatgcacaat     5280

gatgtgtgca aacaacgcaa tcaaaagtct atgcatgctg accaaactct gatcaccaag     5340

ttgcgaacat gaaaaagaag acctgtgtat atataagtaa gggggagagc cctaactaga     5400

tctttcgaaa accccccgac cttcaccttc cacaaccatg atcatcttat acgttttggc     5460

cgttgcggtc tccttcctca tcttcaagag agtcacctac acgatgcgaa gccgagagct     5520

cgccaagaag tggcactgtg aggagcctca caacctgaat gatctagata gcggccgcca     5580

tcttaacggt gtgttcgtta agatgacccg ggacgaggct gcctttgccg agaccgagaa     5640

gctcattaac gcataaaggg atgtatactt gtactgtaga tggattaata aattatttat     5700

gatgaacaaa cattgactga gaaaggcacc atattgtagc ttcaagtact gtactggtag     5760

aagtaagcct gtcgttaccc acttatggag atccaatatt gtttgatgcg gccatcggac     5820

tgcttggagc gtacagagaa ttggtaaaat gtggtggctg ggttggtttc gattgaaatt     5880

atcaagatct ctgaggtatt cttggtgttg aagggatgtt cttggactac tgcttgtaac     5940

atagatggga tgtagggatg gcatggggta taatcgagac aaccattgtg gaggtaggca     6000

agagctctta caagtaccat acgagtacaa ctactgaggc ataatgtctg gaattcccgg     6060

ttaagtccat tacagttgat atcattcaaa tcggacagat attctccgag gtgatacatc     6120

tctgtccccg agagaagctg atttggagca catccaggtt acggggggag agaaatttat     6180

gtaatgtgag gattttttgg agctgaataa taaagtcctg ttgatctagc ttcaattgag     6240

tcaacatccg ccgtcgactg acgtacgggt ggtgagaatg gttggttgta gggaagaatc     6300

aaaggccggt ctcgggatcc gtgggtatat atatatatat atatatatac gatccttcgt     6360

tacctccctg ttctcaaaac tgtggttttt cgtttttcgt tttttgcttt ttttgatttt     6420

tttagggcca actaagcttc cagatttcgc taatcacctt tgtactaatt acaagaaagg     6480

aagaagctga ttagagttgg gctttttatg caactgtgct actccttatc tctgatatga     6540

aagtgtagac ccaatcacat catgtcattt agagttggta atactgggag gatagataag     6600

gcacgaaaac gagccatagc agacatgctg ggtgtagcca agcagaagaa agtagatggg     6660

agccaattga cgagcgaggg agctacgcca atccgacata cgacacgctg agatcgtctt     6720

ggccgggggg tacctacaga tgtccaaggg taagtgcttg actgtaattg tatgtctgag     6780

gacaaatatg tagtcagccg tataaagtca taccaggcac cagtgccatc atcgaaccac     6840

taactctcta tgatacatgc ctccggtatt attgtaccat gcgtcgcttt gttacatacg     6900

tatcttgcct ttttctctca gaaactccag actttggcta ttggtcgaga taagcccgga     6960

ccatagtgag tctttcacac tctgtttaaa caagctacca ccacactcgt tgggtgcagt     7020

cgccagctta aagatatcta tccacatcag ccacaactcc cttcctttaa taaaccgact     7080

acacccttgg ctattgaggt tatgagtgaa tatactgtag acaagacact ttcaagaaga     7140

ctgtttccaa aacgtaccac tgtcctccac tacaaacaca cccaatctgc ttcttctagt     7200

caaggttgct acaccggtaa attataaatc atcatttcat tagcagggca gggccctttt     7260

tatagagtct tatacactag cggaccctgc cggtagacca acccgcaggc gcgtcagttt     7320

gctccttcca tcaatgcgtc gtagactagt tcagatctcg attt                      7364


<210>  102
<211>  9211
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pYPS241

<400>  102
cgatgactgt cgactccaac ttttcacact gagcgtaaaa tgtggagaag aaatcggcac       60

taaaaagtca ggtagactgg aaaatgcgcc atgaaatgaa tatctcttgc tacagtaatg      120

cccagcatcg aggggtattg tgtcaccaac actatagtgg cagctgaagc gctcgtgatt      180

gtagtatgag tctttattgg tgatgggaag agttcactca atattctcgt tactgccaaa      240

acaccacggt aatcggccag acaccatgga tgtagatcac caagcctgtg aatgttattc      300

gagctaaaat gcacatggtt ggtgaaagga gtagttgctg tcgaattccg tcgtcgcctg      360

agtcatcatt tatttaccag ttggccacaa acccttgacg atctcgtatg tcccctccga      420

catactcccg gccggctggg gtacgttcga tagcgctatc ggcatcgaca aggtttgggt      480

ccctagccga taccgcacta cctgagtcac aatcttcgga ggtttagtct tccacatagc      540

acgggcaaaa gtgcgtatat atacaagagc gtttgccagc cacagatttt cactccacac      600

accacatcac acatacaacc acacacatcc acaatggaac ccgaaactaa gaagaccaag      660

actgactcca agaagattgt tcttctcggc ggcgacttct gtggccccga ggtgattgcc      720

gaggccgtca aggtgctcaa gtctgttgct gaggcctccg gcaccgagtt tgtgtttgag      780

gaccgactca ttggaggagc tgccattgag aaggagggcg agcccatcac cgacgctact      840

ctcgacatct gccgaaaggc tgactctatt atgctcggtg ctgtcggagg cgctgccaac      900

accgtatgga ccactcccga cggacgaacc gacgtgcgac ccgagcaggg tctcctcaag      960

ctgcgaaagg acctgaacct gtacgccaac ctgcgaccct gccagctgct gtcgcccaag     1020

ctcgccgatc tctcccccat ccgaaacgtt gagggcaccg acttcatcat tgtccgagag     1080

ctcgtcggag gtatctactt tggagagcga aaggaggatg acggatctgg cgtcgcttcc     1140

gacaccgaga cctactccgt tcctgaggtt gagcgaattg cccgaatggc cgccttcctg     1200

gcccttcagc acaacccccc tcttcccgtg tggtctcttg acaaggccaa cgtgctggcc     1260

tcctctcgac tttggcgaaa gactgtcact cgagtcctca aggacgaatt cccccagctc     1320

gagctcaacc accagctgat cgactcggcc gccatgatcc tcatcaagca gccctccaag     1380

atgaatggta tcatcatcac caccaacatg tttggcgata tcatctccga cgaggcctcc     1440

gtcatccccg gttctctggg tctgctgccc tccgcctctc tggcttctct gcccgacacc     1500

aacgaggcgt tcggtctgta cgagccctgt cacggatctg cccccgatct cggcaagcag     1560

aaggtcaacc ccattgccac cattctgtct gccgccatga tgctcaagtt ctctcttaac     1620

atgaagcccg ccggtgacgc tgttgaggct gccgtcaagg agtccgtcga ggctggtatc     1680

actaccgccg atatcggagg ctcttcctcc acctccgagg tcggagactt gttgccaaca     1740

aggtcaagga gctgctcaag aaggagtaag tcgtttctac gacgcattga tggaaggagc     1800

aaactgacgc gcctgcgggt tggtctaccg gcagggtccg ctagtgtata agactctata     1860

aaaagggccc tgccctgcta atgaaatgat gatttataat ttaccggtgt agcaaccttg     1920

actagaagaa gcagattggg tgtgtttgta gtggaggaca gtggtacgtt ttggaaacag     1980

tcttcttgaa agtgtcttgt ctacagtata ttcactcata acctcaatag ccaagggtgt     2040

agtcggttta ttaaaggaag ggagttgtgg ctgatgtgga tagatatctt taagctggcg     2100

actgcaccca acgagtgtgg tggtagcttg tttaaacggt aggttagtgc ttggtatatg     2160

agttgtaggc atgacaattt ggaaaggggt ggactttggg aatattgtgg gatttcaata     2220

ccttagtttg tacgcaacta acatgaatga atacgatata catcaaagac tatgatacgc     2280

agtattgcac actgtacgag taagagcact agccactgca ctcaagtgaa accgttgccc     2340

gggtacgagt atgagtatgt acagtatgtt tagtattgta cttggacagt gcttgtatcg     2400

tacattctca agtgtcaaac ataaatatcc gttgctatat cctcgcacca ccacgtagct     2460

cgctatatcc ctgtgttgaa tccatccatc ttggattgcc aattgtgcac acagaaccgg     2520

gcactcactt ccccatccac acttgcggcc gcttaggcct tgtctgcctt ggcaaccatt     2580

cgagcgaagg tgcccaggta ggagataaga atgccgacac cttcgagcat gggaggagat     2640

cggtagggca ggttgtgctt cttgcacagc tgctccactt tgatggaggc agcggtgagg     2700

ttgtgtctgg gaagagtagg ccacaggtga tgttcgatct ggtagttgag gccaccgaaa     2760

aaccagtctg tgacgaggcc tcgctgaacg ttcatggtct cgtgaatctg tccagcacag     2820

aagccatgac catcccacac ggagtcctga atcttctcca gagggtagtg gttcatgaag     2880

acaacgatgg caattccgaa gccaccaagc agctcggaga caaagaacac catgagtccg     2940

gtgagaaacg agggcatgta gaaatagtag aacaaggcct tgagacccca gtggagtgcc     3000

agaccgacgg actccttctc gtactgtctt cgatagtact gattgcttcg atccttgaga     3060

cccgtggcag tatgaatgga ctgaaagcac cagatgaatc gcagaagggc acagatgaaa     3120

aagaagtaat actgttggta cttgatcatc cgtcgagaaa acgggccagc tcgctcgacg     3180

tcctccttgg accacgccag gagaggcagg ttgtcgatgt cgggatcgtg accctgcacg     3240

ttggtggcgg agtgatgagc gttgtgtcgg tccttccacc aagtgacaga aaagccctga     3300

agcacgtttc cgaagagcag gccaatggca ttgttgatgg atcggtcctt gaacagctgg     3360

tgatggcaaa tgtcgtgcga gagccatccc atctgttgaa agtgaatgcc gaggacgata     3420

gcaccaacca ggtaccagtg ccactgggtc atcaacagca cggcaaggac acccagtccg     3480

agagtcgaca gggtcttgta agcgtaccac atgggagagg catcgaacat gcctgcagca     3540

atgagctcgt ctcgcagctt tcggaaatcc tcctgaggtt cgtcggagtt tggcttggga     3600

ggtgttggag tcaggggaga ggaaggttcc atgatgggca ttcgtctgag tttggacacg     3660

gcgttctcgg agtgcataac catgaagacg tcggtagcat cccgacctcg gtagttctcg     3720

ataatgtcgg cacctccggg atggtggttg agccaggcag aaacgtcgta ggtgacaccg     3780

tccacggtga gaggcagagc gggtcgcttg accatggttg atgtgtgttt aattcaagaa     3840

tgaatataga gaagagaaga agaaaaaaga ttcaattgag ccaaaccgat ttaaatgagt     3900

atctgtctga ctcgtcattg ccgcctttgg agtacgactc caactatgag tgtgcttgga     3960

tcactttgac gatacattct tcgttggagg ctgtgggtct gacagctgcg ttttcggcgc     4020

ggttggccga caacaatatc agctgcaacg tcattgctgg ctttcatcat gatcacattt     4080

ttgtcggcaa aggcgacgcc cagagagcca ttgacgttct ttctaatttg gaccgatagc     4140

cgtatagtcc agtctatcta taagttcaac taactcgtaa ctattaccat aacatatact     4200

tcactgcccc agataaggtt ccgataaaaa gttctgcaga ctaaatttat ttcagtctcc     4260

tcttcaccac caaaatgccc tcctacgaag ctcgagctaa cgtccacaag tccgcctttg     4320

ccgctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg     4380

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga     4440

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca     4500

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg     4560

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca     4620

acgcccacgg tgtacccgga accggaatca ttgctggcct gcgagctggt gccgaggaaa     4680

ctgtctctga acagaagaag gaggacgtct ctgactacga gaactcccag tacaaggagt     4740

tcctagtccc ctctcccaac gagaagctgg ccagaggtct gctcatgctg gccgagctgt     4800

cttgcaaggg ctctctggcc actggcgagt actccaagca gaccattgag cttgcccgat     4860

ccgaccccga gtttgtggtt ggcttcattg cccagaaccg acctaagggc gactctgagg     4920

actggcttat tctgaccccc ggggtgggtc ttgacgacaa gggagacgct ctcggacagc     4980

agtaccgaac tgttgaggat gtcatgtcta ccggaacgga tatcataatt gtcggccgag     5040

gtctgtacgg ccagaaccga gatcctattg aggaggccaa gcgataccag aaggctggct     5100

gggaggctta ccagaagatt aactgttaga ggttagacta tggatatgta atttaactgt     5160

gtatatagag agcgtgcaag tatggagcgc ttgttcagct tgtatgatgg tcagacgacc     5220

tgtctgatcg agtatgtatg atactgcaca acctgtgtat ccgcatgatc tgtccaatgg     5280

ggcatgttgt tgtgtttctc gatacggaga tgctgggtac agtgctaata cgttgaacta     5340

cttatactta tatgaggctc gaagaaagct gacttgtgta tgacttaatt aacgcggcgc     5400

gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct     5460

cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat     5520

cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga     5580

acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt     5640

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt     5700

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     5760

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     5820

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     5880

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     5940

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     6000

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     6060

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     6120

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     6180

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     6240

tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg     6300

tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta     6360

aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg     6420

aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     6480

tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     6540

gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     6600

agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     6660

aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag     6720

gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     6780

caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     6840

cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     6900

ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     6960

ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac     7020

gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     7080

cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     7140

gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     7200

caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     7260

tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat     7320

acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa     7380

aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc     7440

aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct     7500

cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg     7560

agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact     7620

ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac     7680

cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga     7740

gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga     7800

aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca     7860

ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg     7920

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg     7980

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg     8040

taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccgacgtc     8100

gcatgccact tagtctacag attacgtgtg gtgtactggt acctgtggtg tagcgacagt     8160

attgtacata ctcgtactcc cactctacac acccgccgcc accacttacc ctcgtgagaa     8220

ccccctcaga aactcacaac cgtcacctta gcagaagggt ttagccgctt ggagccgaca     8280

gggatgtcag gcaatacagc tgtcaccgag gggacaccgg ggtgtcaaca acagtgtcat     8340

ttcggtgctt gtggagtgag tgacatcacc gagaaagttg tagtatgtag gggtttttgg     8400

aatggttgtt tcatgatcca ttggtactgc tcggcaaaaa tagcctgatg gaatcacgtt     8460

tcgttctggg gttgacgggc atctcagcta tctccatcca cgagtgtctc agagataaga     8520

cctgcatatc tgttatctgt tttcaaagtg taagtcgtat agagtgccgg gagggggtca     8580

aggatgtgca tggacgtgac tacagtgaga gtttttggag gaagtaaaga ccgagtatcg     8640

accatccaga aaccccatat cttctggaat tccacaaatg gccaagactg gcttctttta     8700

tccacttttt gatactaaaa atgtctaatt tgacctctct tccactaaca aaaacagtct     8760

cgccgttctc caacccgcac caccaactac aagtacagta gctataattt tttggcacca     8820

ctcgcttttc tctttttagg cgaaacttgt ctcaaactta tcatgccata ggcattaatt     8880

tccgggtctt gcagagctac tgcactgggg ggggggggtg tacaacgcac gaccgagtgc     8940

tatgtaatgt acacaatggc cacaaacaca actgttactg atgtaccgat gactcagcac     9000

ctacagtagt ccacgcgttt gacttccggg gctcttcggg cccaattttt ttcttcgtct     9060

gcttcccaaa tgccagattt atgcaaggtg catgtgacgt cccggtccga ggattgctat     9120

aacgggcgaa ataaggacac aaacaactca aaaataatac caaaatatag cttgcaacaa     9180

gggcttcaga ttgatcccat tattggtcca t                                    9211


<210>  103
<211>  1260
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1260)
<223>  synthetic delta-8 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<400>  103
atg gtc aag cga ccc gct ctg cct ctc acc gtg gac ggt gtc acc tac         48
Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr           
1               5                   10                  15                

gac gtt tct gcc tgg ctc aac cac cat ccc gga ggt gcc gac att atc         96
Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile           
            20                  25                  30                    

gag aac tac cga ggt cgg gat gct acc gac gtc ttc atg gtt atg cac        144
Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His           
        35                  40                  45                        

tcc gag aac gcc gtg tcc aaa ctc aga cga atg ccc atc atg gaa cct        192
Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro           
    50                  55                  60                            

tcc tct ccc ctg act cca aca cct ccc aag cca aac tcc gac gaa cct        240
Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro           
65                  70                  75                  80            

cag gag gat ttc cga aag ctg cga gac gag ctc att gct gca ggc atg        288
Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met           
                85                  90                  95                

ttc gat gcc tct ccc atg tgg tac gct tac aag acc ctg tcg act ctc        336
Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu           
            100                 105                 110                   

gga ctg ggt gtc ctt gcc gtg ctg ttg atg acc cag tgg cac tgg tac        384
Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr           
        115                 120                 125                       

ctg gtt ggt gct atc gtc ctc ggc att cac ttt caa cag atg gga tgg        432
Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp           
    130                 135                 140                           

ctc tcg cac gac att tgc cat cac cag ctg ttc aag gac cga tcc atc        480
Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile           
145                 150                 155                 160           

aac aat gcc att ggc ctg ctc ttc gga aac gtg ctt cag ggc ttt tct        528
Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser           
                165                 170                 175               

gtc act tgg tgg aag gac cga cac aac gct cat cac tcc gcc acc aac        576
Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn           
            180                 185                 190                   

gtg cag ggt cac gat ccc gac atc gac aac ctg cct ctc ctg gcg tgg        624
Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp           
        195                 200                 205                       

tcc aag gag gac gtc gag cga gct ggc ccg ttt tct cga cgg atg atc        672
Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile           
    210                 215                 220                           

aag tac caa cag tat tac ttc ttt ttc atc tgt gcc ctt ctg cga ttc        720
Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe           
225                 230                 235                 240           

atc tgg tgc ttt cag tcc att cat act gcc acg ggt ctc aag gat cga        768
Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg           
                245                 250                 255               

agc aat cag tac tat cga aga cag tac gag aag gag tcc gtc ggt ctg        816
Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu           
            260                 265                 270                   

gca ctc cac tgg ggt ctc aag gcc ttg ttc tac tat ttc tac atg ccc        864
Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro           
        275                 280                 285                       

tcg ttt ctc acc gga ctc atg gtg ttc ttt gtc tcc gag ctg ctt ggt        912
Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly           
    290                 295                 300                           

ggc ttc gga att gcc atc gtt gtc ttc atg aac cac tac cct ctg gag        960
Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu           
305                 310                 315                 320           

aag att cag gac tcc gtg tgg gat ggt cat ggc ttc tgt gct gga cag       1008
Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln           
                325                 330                 335               

att cac gag acc atg aac gtt cag cga ggc ctc gtc aca gac tgg ttt       1056
Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe           
            340                 345                 350                   

ttc ggt ggc ctc aac tac cag atc gaa cat cac ctg tgg cct act ctt       1104
Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu           
        355                 360                 365                       

ccc aga cac aac ctc acc gct gcc tcc atc aaa gtg gag cag ctg tgc       1152
Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys           
    370                 375                 380                           

aag aag cac aac ctg ccc tac cga tct cct ccc atg ctc gaa ggt gtc       1200
Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val           
385                 390                 395                 400           

ggc att ctt atc tcc tac ctg ggc acc ttc gct cga atg gtt gcc aag       1248
Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys           
                405                 410                 415               

gca gac aag gcc                                                       1260
Ala Asp Lys Ala                                                           
            420                                                           


<210>  104
<211>  420
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  104

Met Val Lys Arg Pro Ala Leu Pro Leu Thr Val Asp Gly Val Thr Tyr 
1               5                   10                  15      


Asp Val Ser Ala Trp Leu Asn His His Pro Gly Gly Ala Asp Ile Ile 
            20                  25                  30          


Glu Asn Tyr Arg Gly Arg Asp Ala Thr Asp Val Phe Met Val Met His 
        35                  40                  45              


Ser Glu Asn Ala Val Ser Lys Leu Arg Arg Met Pro Ile Met Glu Pro 
    50                  55                  60                  


Ser Ser Pro Leu Thr Pro Thr Pro Pro Lys Pro Asn Ser Asp Glu Pro 
65                  70                  75                  80  


Gln Glu Asp Phe Arg Lys Leu Arg Asp Glu Leu Ile Ala Ala Gly Met 
                85                  90                  95      


Phe Asp Ala Ser Pro Met Trp Tyr Ala Tyr Lys Thr Leu Ser Thr Leu 
            100                 105                 110         


Gly Leu Gly Val Leu Ala Val Leu Leu Met Thr Gln Trp His Trp Tyr 
        115                 120                 125             


Leu Val Gly Ala Ile Val Leu Gly Ile His Phe Gln Gln Met Gly Trp 
    130                 135                 140                 


Leu Ser His Asp Ile Cys His His Gln Leu Phe Lys Asp Arg Ser Ile 
145                 150                 155                 160 


Asn Asn Ala Ile Gly Leu Leu Phe Gly Asn Val Leu Gln Gly Phe Ser 
                165                 170                 175     


Val Thr Trp Trp Lys Asp Arg His Asn Ala His His Ser Ala Thr Asn 
            180                 185                 190         


Val Gln Gly His Asp Pro Asp Ile Asp Asn Leu Pro Leu Leu Ala Trp 
        195                 200                 205             


Ser Lys Glu Asp Val Glu Arg Ala Gly Pro Phe Ser Arg Arg Met Ile 
    210                 215                 220                 


Lys Tyr Gln Gln Tyr Tyr Phe Phe Phe Ile Cys Ala Leu Leu Arg Phe 
225                 230                 235                 240 


Ile Trp Cys Phe Gln Ser Ile His Thr Ala Thr Gly Leu Lys Asp Arg 
                245                 250                 255     


Ser Asn Gln Tyr Tyr Arg Arg Gln Tyr Glu Lys Glu Ser Val Gly Leu 
            260                 265                 270         


Ala Leu His Trp Gly Leu Lys Ala Leu Phe Tyr Tyr Phe Tyr Met Pro 
        275                 280                 285             


Ser Phe Leu Thr Gly Leu Met Val Phe Phe Val Ser Glu Leu Leu Gly 
    290                 295                 300                 


Gly Phe Gly Ile Ala Ile Val Val Phe Met Asn His Tyr Pro Leu Glu 
305                 310                 315                 320 


Lys Ile Gln Asp Ser Val Trp Asp Gly His Gly Phe Cys Ala Gly Gln 
                325                 330                 335     


Ile His Glu Thr Met Asn Val Gln Arg Gly Leu Val Thr Asp Trp Phe 
            340                 345                 350         


Phe Gly Gly Leu Asn Tyr Gln Ile Glu His His Leu Trp Pro Thr Leu 
        355                 360                 365             


Pro Arg His Asn Leu Thr Ala Ala Ser Ile Lys Val Glu Gln Leu Cys 
    370                 375                 380                 


Lys Lys His Asn Leu Pro Tyr Arg Ser Pro Pro Met Leu Glu Gly Val 
385                 390                 395                 400 


Gly Ile Leu Ile Ser Tyr Leu Gly Thr Phe Ala Arg Met Val Ala Lys 
                405                 410                 415     


Ala Asp Lys Ala 
            420 


<210>  105
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  EgD5M (i.e., EgD5R*-34g158g) mutant delta-5 desaturase

<400>  105
atg gcc ctg tct ctc act acc gaa cag ctc ctg gag cga cct gat ctc         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtc gct atc gat ggt atc ctg tac gac ctc gag ggc ctg gcc aag gtg         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ggt ggt gga gac ctc att ctg gcc tct gga gcc tcc gac gcc tct        144
His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

ccc ctc ttc tac tct atg cat ccc tac gtc aag ccc gag aac tcc aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctc ctg cag caa ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg ggt cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag cga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  106
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  106

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  107
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  EgD5M1 (i.e., EgD5R*-34g158g347s) mutant delta-5 desaturase

<400>  107
atg gcc ctg tct ctc act acc gaa cag ctc ctg gag cga cct gat ctc         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtc gct atc gat ggt atc ctg tac gac ctc gag ggc ctg gcc aag gtg         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ggt ggt gga gac ctc att ctg gcc tct gga gcc tcc gac gcc tct        144
His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

ccc ctc ttc tac tct atg cat ccc tac gtc aag ccc gag aac tcc aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctc ctg cag caa ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg ggt cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag agc tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  108
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  108

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  109
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  EgD5S-36s157g mutant delta-5 desaturase

<400>  109
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga tcc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gat ggt tct cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Gly Ser His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  110
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  110

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Gly Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  111
<211>  1365
<212>  DNA
<213>  Euglena anabaena


<220>
<221>  CDS
<222>  (1)..(1365)
<223>  EaD5S-35a158g mutant delta-5 desaturase

<400>  111
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtc cat gcc gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga        144
Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat ggt tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Gly Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gag ttc taa                                           1365
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  112
<211>  454
<212>  PRT
<213>  Euglena anabaena

<400>  112

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Gly Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  113
<211>  13926
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZR5AU-555

<400>  113
cgatccctaa ctgtatacta gcattctgaa ttaatatctt cctctttgat tgttccttag       60

gcatatcagc ggggatcccg tcaacagttt tatatatcgt agttacaacc atcaacactt      120

tttggtaagt gtaccattct atactccaac tggtctgcaa ctgtacaagt agacatgtta      180

atggtagtta ataacatcta cagcagaacc tatggtaaag acattgcatt tttacaggaa      240

gtatcgtcct acacgttgat aaatccaaag atgcggaact tcttccactt ttatcatcat      300

cccctactcg tacactcgta ctctttgttc gatcgcgatt catttctata aataatcttg      360

tatgtacatg cggccgctta gaactcctgg gcatcctgaa tggtgtgagc aacacccacc      420

ttctgcatgt atcgaagagt ggacatgatg ttctcgagaa tggtaggata gtatgtgtaa      480

cggacgccat gtcgcttgca acactcccga acggtgtctt gaatgaaggg atagtgccaa      540

gaggacattc ggggaaacaa gtgatgctcg atctgcaggt tgagaccgcc tgtaagcatc      600

attcccacgc tgccaccgta ggtggcagca gtctcggcct gtgctcggta ccagtcgact      660

cgatcctctt ccacgtctct gacctccttg ccttccttag ctcgtttggc cttgcagttc      720

ttctctgggg tccgttcggc tccctcgaag ttatgggaca gaacgaacac aaaggtgagg      780

aagatgcctg tgaccgtggg caccagaaga atggtgagca gaggagaggc accagcaatg      840

taccagggaa gaaaggcgtt tcggaagatg aagagcagtc gaagggaaac ggcgaaagct      900

cgctgccgat tgaaaaagct gcctggtctg agagaagtgg cctcgggaat ggtgtcgttg      960

tgctgcattg tgaagaggta catgggattg tacaccatcg agacaccata cattcccaga     1020

acgatgtaca tgtaccacgc ctgaaatcgg tggtaccact ttcgagaggc gttggtagca     1080

ggataggagt ggaacaagat gaacggctct gccgaggcag catccaggtc gaggccatgc     1140

tggttggtgt aagtgtgatg tcgcataatg tgggactgca gccagatcca acgagaggaa     1200

ccaatggcgt cgatgccata agcgaaaaag gcgttgaccc agggcttttt gctgatggct     1260

ccgtgagaac catcgtgttg aatggccaag ccaatctgcg agtggaagta tccagtaaag     1320

ataccccaca tcactgctcc ggttgtgatc cagtaccact cgccaatggc agtacaagcg     1380

atgatgagtg cggttctcag ccagaagcca ggagtggcgt accagtttcg acccttcatg     1440

acctctcgaa cggactgctt gacgtcctga gcaaagggag agtcgaaggt gtacacggga     1500

tccttggagc ttcgttcgtg cttgcctcgg gcaaattgct gcagaagctt cgagttctct     1560

ggcttgactc caggatgcat ggagtagaac agagcggttc cgtcggatgc accggcagct     1620

tcgatgaggt tgcctccggc atggaccttg gccagaccga agagatcgta caggactccg     1680

tcgatggcaa cgagctcggg gtgttccagg agctgctcgg tagtcaggga gatggtggcc     1740

atggttgtga attagggtgg tgagaatggt tggttgtagg gaagaatcaa aggccggtct     1800

cgggatccgt gggtatatat atatatatat atatatacga tccttcgtta cctccctgtt     1860

ctcaaaactg tggtttttcg tttttcgttt tttgcttttt ttgatttttt tagggccaac     1920

taagcttcca gatttcgcta atcacctttg tactaattac aagaaaggaa gaagctgatt     1980

agagttgggc tttttatgca actgtgctac tccttatctc tgatatgaaa gtgtagaccc     2040

aatcacatca tgtcatttag agttggtaat actgggagga tagataaggc acgaaaacga     2100

gccatagcag acatgctggg tgtagccaag cagaagaaag tagatgggag ccaattgacg     2160

agcgagggag ctacgccaat ccgacatacg acacgctgag atcgtcttgg ccggggggta     2220

cctacagatg tccaagggta agtgcttgac tgtaattgta tgtctgagga caaatatgta     2280

gtcagccgta taaagtcata ccaggcacca gtgccatcat cgaaccacta actctctatg     2340

atacatgcct ccggtattat tgtaccatgc gtcgctttgt tacatacgta tcttgccttt     2400

ttctctcaga aactccagac tttggctatt ggtcgagata agcccggacc atagtgagtc     2460

tttcacactc tgtttaaaca agctaccacc acactcgttg ggtgcagtcg acgagtatct     2520

gtctgactcg tcattgccgc ctttggagta cgactccaac tatgagtgtg cttggatcac     2580

tttgacgata cattcttcgt tggaggctgt gggtctgaca gctgcgtttt cggcgcggtt     2640

ggccgacaac aatatcagct gcaacgtcat tgctggcttt catcatgatc acatttttgt     2700

cggcaaaggc gacgcccaga gagccattga cgttctttct aatttggacc gatagccgta     2760

tagtccagtc tatctataag ttcaactaac tcgtaactat taccataaca tatacttcac     2820

tgccccagat aaggttccga taaaaagttc tgcagactaa atttatttca gtctcctctt     2880

caccaccaaa atgccctcct acgaagctcg agctaacgtc cacaagtccg cctttgccgc     2940

tcgagtgctc aagctcgtgg cagccaagaa aaccaacctg tgtgcttctc tggatgttac     3000

caccaccaag gagctcattg agcttgccga taaggtcgga ccttatgtgt gcatgatcaa     3060

aacccatatc gacatcattg acgacttcac ctacgccggc actgtgctcc ccctcaagga     3120

acttgctctt aagcacggtt tcttcctgtt cgaggacaga aagttcgcag atattggcaa     3180

cactgtcaag caccagtacc ggtgtcaccg aatcgccgag tggtccgata tcaccaacgc     3240

ccacggtgta cccggaaccg gaatcattgc tggcctgcga gctggtgccg aggaaactgt     3300

ctctgaacag aagaaggagg acgtctctga ctacgagaac tcccagtaca aggagttcct     3360

agtcccctct cccaacgaga agctggccag aggtctgctc atgctggccg agctgtcttg     3420

caagggctct ctggccactg gcgagtactc caagcagacc attgagcttg cccgatccga     3480

ccccgagttt gtggttggct tcattgccca gaaccgacct aagggcgact ctgaggactg     3540

gcttattctg acccccgggg tgggtcttga cgacaaggga gacgctctcg gacagcagta     3600

ccgaactgtt gaggatgtca tgtctaccgg aacggatatc ataattgtcg gccgaggtct     3660

gtacggccag aaccgagatc ctattgagga ggccaagcga taccagaagg ctggctggga     3720

ggcttaccag aagattaact gttagaggtt agactatgga tatgtaattt aactgtgtat     3780

atagagagcg tgcaagtatg gagcgcttgt tcagcttgta tgatggtcag acgacctgtc     3840

tgatcgagta tgtatgatac tgcacaacct gtgtatccgc atgatctgtc caatggggca     3900

tgttgttgtg tttctcgata cggagatgct gggtacagtg ctaatacgtt gaactactta     3960

tacttatatg aggctcgaag aaagctgact tgtgtatgac ttattctcaa ctacatcccc     4020

agtcacaata ccaccactgc actaccacta caccaaaacc atgatcaaac caccgatgga     4080

cttcctggag gcagaagaac ttgttatgga aaagctcaag agagagaatt caggagagac     4140

cgggttggcg gcgtatttgt gtcccaaaaa acagccccaa ttgccccgga gaagacggcc     4200

aggccgccta gatgacaaat tcaacaactc acagctgact ttctgccatt gccactaggg     4260

gggggccttt ttatatggcc aagccaagct ctccacgtcg gttgggctgc acccaacaat     4320

aaatgggtag ggttgcacca acaaagggat gggatggggg gtagaagata cgaggataac     4380

ggggctcaat ggcacaaata agaacgaata ctgccattaa gactcgtgat ccagcgactg     4440

acaccattgc atcatctaag ggcctcaaaa ctacctcgga actgctgcgc tgatctggac     4500

accacagagg ttccgagcac tttaggttgc accaaatgtc ccaccaggtg caggcagaaa     4560

acgctggaac agcgtgtaca gtttgtctta acaaaaagtg agggcgctga ggtcgagcag     4620

ggtggtgtga cttgttatag cctttagagc tgcgaaagcg cgtatggatt tggctcatca     4680

ggccagattg agggtctgtg gacacatgtc atgttagtgt acttcaatcg ccccctggat     4740

atagccccga caataggccg tggcctcatt tttttgcctt ccgcacattt ccattgctcg     4800

gtacccacac cttgcttctc ctgcacttgc caaccttaat actggtttac attgaccaac     4860

atcttacaag cggggggctt gtctagggta tatataaaca gtggctctcc caatcggttg     4920

ccagtctctt ttttcctttc tttccccaca gattcgaaat ctaaactaca catcacacaa     4980

tgcctgttac tgacgtcctt aagcgaaagt ccggtgtcat cgtcggcgac gatgtccgag     5040

ccgtgagtat ccacgacaag atcagtgtcg agacgacgcg ttttgtgtaa tgacacaatc     5100

cgaaagtcgc tagcaacaca cactctctac acaaactaac ccagctctcc atggctctct     5160

cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac ggcattctct     5220

acgatctgga aggtcttgcc aaggtccatc ccggatccga cttgatcctc gcttctggtg     5280

cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc gagaactcga     5340

agctgcttca acagttcgtg cgaggcaagc acgaccgaac ctccaaggac attgtctaca     5400

cctacgactc tccctttgca caggacgtca agcgaactat gcgagaggtc atgaaaggtc     5460

ggaactggta tgccacacct ggattctggc tgcgaaccgt tggcatcatt gctgtcaccg     5520

ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc ttgactggat     5580

tcatgcacat gcagatcggc ctgtccattc agcacgatgg ttctcatggt gccatcagca     5640

aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga tcgtccagat     5700

ggatctggct gcagtctcac atcatgcgac atcacaccta caccaatcag catggtctcg     5760

acctggatgc cgagtccgca gaaccattcc ttgtgttcca caactaccct gctgccaaca     5820

ctgctcgaaa gtggtttcac cgattccagg cctggtacat gtacctcgtg cttggagcct     5880

acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac aacgacacca     5940

ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac cgaacccttg     6000

cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg tatctcactg     6060

gaacctccct gctcatcacc attcctctgg tgcccactgc taccggtgcc ttcctcacct     6120

tctttttcat cttgtctcac aacttcgatg gctcggagcg aatccccgac aagaactgca     6180

aggtcaagag ctccgagaag gacgttgaag ccgatcagat cgactggtac agagctcagg     6240

tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc ggtctcaact     6300

tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc ttcgtgcagc     6360

aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac cctaccattg     6420

tgggcaacat catttccacc ctcaagtaca tgcacaaagt cggtgtggtt cactgtgtca     6480

aggacgctca ggattcctaa gcggccgcaa gtgtggatgg ggaagtgagt gcccggttct     6540

gtgtgcacaa ttggcaatcc aagatggatg gattcaacac agggatatag cgagctacgt     6600

ggtggtgcga ggatatagca acggatattt atgtttgaca cttgagaatg tacgatacaa     6660

gcactgtcca agtacaatac taaacatact gtacatactc atactcgtac ccgggcaacg     6720

gtttcacttg agtgcagtgg ctagtgctct tactcgtaca gtgtgcaata ctgcgtatca     6780

tagtctttga tgtatatcgt attcattcat gttagttgcg tacgacagac ggacaatgac     6840

gagccaggtt cgcccaactg acggaatctc ctatagtctt ctcatcaagc gcataaatat     6900

agtatataaa gaattacatg gtgggccaca gcttggcgat agtatgacgg tctgggctcc     6960

agatcacacc catgagggat gccgtgcgag tagatgtaga ggtggtgctt ccaggttgtc     7020

cccaacaaca atcgctactg gaaaataccg tgaaagtacg tacacccaac tctgtgactc     7080

gcgttgactt tcatctttca atttggtcgt gtcttacggt gcatgtgctg tattggacat     7140

gaagaagggg ttggcaagct gaaccatgcc tgaggtgctg gagtctgata ctgtctagca     7200

gagggcgatc aaaagatcac catccacacg tttgtttcca tattcaatct ccaatttcca     7260

acatctcctt ggctaatagc cactttgaga gatatttggt atcgcgtatt cacttgtctc     7320

atatcccgtg tttctgtttc accaaggctt ggacgatatt atcgcgagaa aaaccgtatt     7380

gaggtattta ttagagtcga gagaggagga acaggtattc ggaagatgga aaagctgtac     7440

agagcgagaa acgatcccat gtcgacgctc ctgatggctt atactgagac atggaatcag     7500

gatcttgccc ttcttcacag ctctacttgc agctacagaa ttgctttttc tcgtatccga     7560

tcataattcc gctgctatga taggcagcca ccgatacaat ataggagaga gtataggtat     7620

tgactctata gacactgtat gatgctgaca aagctgactg agggattcga ctcagcttcg     7680

ccgttgcttg atctccaatt aattatcgta ggcgcgccag ctgcattaat gaatcggcca     7740

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     7800

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     7860

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     7920

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     7980

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     8040

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     8100

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     8160

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     8220

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     8280

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     8340

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac     8400

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     8460

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     8520

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     8580

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     8640

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     8700

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     8760

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     8820

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     8880

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     8940

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     9000

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     9060

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     9120

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     9180

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     9240

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     9300

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     9360

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     9420

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     9480

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     9540

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     9600

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     9660

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa     9720

taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt     9780

gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat     9840

cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt     9900

ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt     9960

ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag    10020

gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg    10080

aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc    10140

gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc    10200

gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg    10260

cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt    10320

tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa    10380

tacgactcac tatagggcga attgggcccg acgtcgcatg cattaccaac gttggcgcgc    10440

cacaacgtca gaaactctct tccatccgat aagccgtgtc taactatacg accctcaaac    10500

aaggtcagcc agaccaactt ttagcactcg caaaacacac acccaaaccc aggccgcgac    10560

gcagccggag cccgtctact tatactgttt cgccgcagac gccaagtgac acaaaaagcg    10620

cacaaaccct cccccccagt ggcggcgcac atccacaaca aacgacccat aaacggcact    10680

cccacacgga aacaaaacgt ttgtcgggtt actcctcacg ccaagacaca ctcacacaca    10740

caacctccca cctttcatca ccactcaact acgggtacag tagtggtaga aaacacagag    10800

tccagagtcc acacagacac cgcacagagt ctgtcacaca agcaccatgc caacaagcag    10860

accgttcatt tcgctgctgt ttggaagctt ctccacgtcg tatcaacgca acaccaccac    10920

acagacctcc gcatcgacag ccaccgtcac caccggcccc acgtcgcaga tgtacaagat    10980

gctcgacaac cactcgatac ctgagcagcc ccagcagccg accctgactc agacacagcc    11040

agcggtgcag acggcgcaga cggcgcagac ggcgcagcag gcaagtggga ctgcggacct    11100

cagcgaatcg ccacacgaca agctgtggat tggaggccga tcaaacgacg gacgggaaaa    11160

gttctaccgg ctggaaccgg ccaagcgaag ggcatccttc gatcgcattt ctctggatca    11220

ggtgtctatt tgatgaggcg ccaaacagtg gccagaccaa gtctggtatc tcaacccctc    11280

aaaattgata accaagtttc tggtggagtt caaaccaagg ggagctacgg agccgttcac    11340

aaggtgtcca cagaggttca aagcacattg caccaagacc gccatcatcc ttaattaaaa    11400

ggcgttgaaa cagaatgagc cagacagcaa ggacaaggtg gccaacagca aggagtccaa    11460

aaagccctct attgacgaga tccacgatgt tattgctcat gaggtttccg agctcgatgc    11520

tgggaagaag aagtgatttg tatataagaa ataaatgaga tatagtaaag gagtgcaaga    11580

gaatggcaag gtggtcaaat tctatattac ttgcagtcac tggttcctcg ttgacatgaa    11640

tgaagttacc gttggcatag ctgatttaat atataactgt ccaactaact ctcacctaga    11700

tataacccat gtgtgtgttt ccaatcatca atgcggccgc ttaggaatcc tgtgcgtcct    11760

tcacgcagtg gacgacaccc accttatgca tgtacttcag ggtggagatg atgttgccga    11820

cgatggtagg gtagaaaaca tatcgcactc catgtcgttc gcaacactcc cggaccgcct    11880

gctggacgaa ggggtagtgc caagacgaca tccggggaaa gaggtggtgc tcgatctgga    11940

aattgagacc gccagtgaag aacatggcga tggggccacc gtatgtggag gacgtctcca    12000

cctgcgcccg ataccagtca atttggtcag cctcaacgtc cttctcagat cgcttaacct    12060

tgcagttctt gtcggggatc cgttcggagc catcaaaatt gtgggacaaa atgaagaaga    12120

acgtcaagaa ggcaccagtt gcggtgggca ccagaggaat ggtgatcagc aatgaggtcc    12180

cagtgaggta ccagggcaag aatgcggtcc ggaagatgaa gaaagctcgc atcacgaatg    12240

caagtgtgcg gtagcgccgc agaaagccat tttcccgcat ggccgtgaca gactctggga    12300

tggtgtcatt gtgctgcatc cggaaaatgt agagcgggtt gtacaccagc gataccccgt    12360

atgcccccag cacaaggtac atgtaccaag cctggaagcg gtggaaccac tttcgggcgg    12420

tgtttgcggc ggggtagttg tggaacacca ggaacggctc tgccgactcc gcatccaggt    12480

cgaggccgtg ctggttggtg taggtgtggt gccgcatgat gtgcgactgc agccaaatcc    12540

accgggacga tccgatgacg tcaatgccgt aggcgaagag ggcgttgacc caaggcttct    12600

tgctgatggc cccgtgaccc gcatcatgct ggatggataa gccgatctgc atgtgcatga    12660

atccagtcaa caggccccac agcaccatcc ccgtggtagc ccagtgccac tcgcaaaagg    12720

ccgtcacggc gatgatccca acggtgcgca gccagaagcc aggggttgcg taccagttcc    12780

tccctttcat cacctcgcgc attgtccgct taacgtcttg tgcgaaggga gaatcatacg    12840

tgtagacaat gtccttcgag gtgcggtcat gcttccctcg gacgaattgc tgcaggagct    12900

tggagttctc gggcttgacg tagggatgca tagagtagaa gaggggagag gcgtcggagg    12960

ctccagaggc cagaatgagg tctccaccac catgcacctt ggccaggccc tcgaggtcgt    13020

acaggatacc atcgatagcg acgagatcag gtcgctccag gagctgttcg gtagtgagag    13080

acagggccat ggccattgct gtagatatgt cttgtgtgta agggggttgg ggtggttgtt    13140

tgtgttcttg acttttgtgt tagcaaggga agacgggcaa aaaagtgagt gtggttggga    13200

gggagagacg agccttatat ataatgcttg tttgtgtttg tgcaagtgga cgccgaaacg    13260

ggcaggagcc aaactaaaca aggcagacaa tgcgagctta attggattgc ctgatgggca    13320

ggggttaggg ctcgatcaat gggggtgcga agtgacaaaa ttgggaatta ggttcgcaag    13380

caaggctgac aagactttgg cccaaacatt tgtacgcggt ggacaacagg agccacccat    13440

cgtctgtcac gggctagccg gtcgtgcgtc ctgtcaggct ccacctaggc tccatgccac    13500

tccatacaat cccactagtg taccgctagg ccgcttttag ctcccatcta agaccccccc    13560

aaaacctcca ctgtacagtg cactgtactg tgtggcgatc aagggcaagg gaaaaaaggc    13620

gcaaacatgc acgcatggaa tgacgtaggt aaggcgttac tagactgaaa agtggcacat    13680

ttcggcgtgc caaagggtcc taggtgcgtt tcgcgagctg ggcgccaggc caagccgctc    13740

caaaacgcct ctccgactcc ctccagcggc ctccatatcc ccatccctct ccacagcaat    13800

gttgttaagc cttgcaaacg aaaaaataga aaggctaata agcttccaat attgtggtgt    13860

acgctgcata acgcaacaat gagcgccaaa caacacacac acacagcaca cagcagcatt    13920

aaccat                                                               13926


<210>  114
<211>  13926
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZR5AU-555m

<400>  114
cgatccctaa ctgtatacta gcattctgaa ttaatatctt cctctttgat tgttccttag       60

gcatatcagc ggggatcccg tcaacagttt tatatatcgt agttacaacc atcaacactt      120

tttggtaagt gtaccattct atactccaac tggtctgcaa ctgtacaagt agacatgtta      180

atggtagtta ataacatcta cagcagaacc tatggtaaag acattgcatt tttacaggaa      240

gtatcgtcct acacgttgat aaatccaaag atgcggaact tcttccactt ttatcatcat      300

cccctactcg tacactcgta ctctttgttc gatcgcgatt catttctata aataatcttg      360

tatgtacatg cggccgctta gaactcctgg gcatcctgaa tggtgtgagc aacacccacc      420

ttctgcatgt atcgaagagt ggacatgatg ttctcgagaa tggtaggata gtatgtgtaa      480

cggacgccat gtcgcttgca acactcccga acggtgtctt gaatgaaggg atagtgccaa      540

gaggacattc ggggaaacaa gtgatgctcg atctgcaggt tgagaccgcc tgtaagcatc      600

attcccacgc tgccaccgta ggtggcagca gtctcggcct gtgctcggta ccagtcgact      660

cgatcctctt ccacgtctct gacctccttg ccttccttag ctcgtttggc cttgcagttc      720

ttctctgggg tccgttcggc tccctcgaag ttatgggaca gaacgaacac aaaggtgagg      780

aagatgcctg tgaccgtggg caccagaaga atggtgagca gaggagaggc accagcaatg      840

taccagggaa gaaaggcgtt tcggaagatg aagagcagtc gaagggaaac ggcgaaagct      900

cgctgccgat tgaaaaagct gcctggtctg agagaagtgg cctcgggaat ggtgtcgttg      960

tgctgcattg tgaagaggta catgggattg tacaccatcg agacaccata cattcccaga     1020

acgatgtaca tgtaccacgc ctgaaatcgg tggtaccact ttcgagaggc gttggtagca     1080

ggataggagt ggaacaagat gaacggctct gccgaggcag catccaggtc gaggccatgc     1140

tggttggtgt aagtgtgatg tcgcataatg tgggactgca gccagatcca acgagaggaa     1200

ccaatggcgt cgatgccata agcgaaaaag gcgttgaccc agggcttttt gctgatggct     1260

ccgtgagaac catcgtgttg aatggccaag ccaatctgcg agtggaagta tccagtaaag     1320

ataccccaca tcactgctcc ggttgtgatc cagtaccact cgccaatggc agtacaagcg     1380

atgatgagtg cggttctcag ccagaagcca ggagtggcgt accagtttcg acccttcatg     1440

acctctcgaa cggactgctt gacgtcctga gcaaagggag agtcgaaggt gtacacggga     1500

tccttggagc ttcgttcgtg cttgcctcgg gcaaattgct gcagaagctt cgagttctct     1560

ggcttgactc caggatgcat ggagtagaac agagcggttc cgtcggatgc accggcagct     1620

tcgatgaggt tgcctccggc atggaccttg gccagaccga agagatcgta caggactccg     1680

tcgatggcaa cgagctcggg gtgttccagg agctgctcgg tagtcaggga gatggtggcc     1740

atggttgtga attagggtgg tgagaatggt tggttgtagg gaagaatcaa aggccggtct     1800

cgggatccgt gggtatatat atatatatat atatatacga tccttcgtta cctccctgtt     1860

ctcaaaactg tggtttttcg tttttcgttt tttgcttttt ttgatttttt tagggccaac     1920

taagcttcca gatttcgcta atcacctttg tactaattac aagaaaggaa gaagctgatt     1980

agagttgggc tttttatgca actgtgctac tccttatctc tgatatgaaa gtgtagaccc     2040

aatcacatca tgtcatttag agttggtaat actgggagga tagataaggc acgaaaacga     2100

gccatagcag acatgctggg tgtagccaag cagaagaaag tagatgggag ccaattgacg     2160

agcgagggag ctacgccaat ccgacatacg acacgctgag atcgtcttgg ccggggggta     2220

cctacagatg tccaagggta agtgcttgac tgtaattgta tgtctgagga caaatatgta     2280

gtcagccgta taaagtcata ccaggcacca gtgccatcat cgaaccacta actctctatg     2340

atacatgcct ccggtattat tgtaccatgc gtcgctttgt tacatacgta tcttgccttt     2400

ttctctcaga aactccagac tttggctatt ggtcgagata agcccggacc atagtgagtc     2460

tttcacactc tgtttaaaca agctaccacc acactcgttg ggtgcagtcg acgagtatct     2520

gtctgactcg tcattgccgc ctttggagta cgactccaac tatgagtgtg cttggatcac     2580

tttgacgata cattcttcgt tggaggctgt gggtctgaca gctgcgtttt cggcgcggtt     2640

ggccgacaac aatatcagct gcaacgtcat tgctggcttt catcatgatc acatttttgt     2700

cggcaaaggc gacgcccaga gagccattga cgttctttct aatttggacc gatagccgta     2760

tagtccagtc tatctataag ttcaactaac tcgtaactat taccataaca tatacttcac     2820

tgccccagat aaggttccga taaaaagttc tgcagactaa atttatttca gtctcctctt     2880

caccaccaaa atgccctcct acgaagctcg agctaacgtc cacaagtccg cctttgccgc     2940

tcgagtgctc aagctcgtgg cagccaagaa aaccaacctg tgtgcttctc tggatgttac     3000

caccaccaag gagctcattg agcttgccga taaggtcgga ccttatgtgt gcatgatcaa     3060

aacccatatc gacatcattg acgacttcac ctacgccggc actgtgctcc ccctcaagga     3120

acttgctctt aagcacggtt tcttcctgtt cgaggacaga aagttcgcag atattggcaa     3180

cactgtcaag caccagtacc ggtgtcaccg aatcgccgag tggtccgata tcaccaacgc     3240

ccacggtgta cccggaaccg gaatcattgc tggcctgcga gctggtgccg aggaaactgt     3300

ctctgaacag aagaaggagg acgtctctga ctacgagaac tcccagtaca aggagttcct     3360

agtcccctct cccaacgaga agctggccag aggtctgctc atgctggccg agctgtcttg     3420

caagggctct ctggccactg gcgagtactc caagcagacc attgagcttg cccgatccga     3480

ccccgagttt gtggttggct tcattgccca gaaccgacct aagggcgact ctgaggactg     3540

gcttattctg acccccgggg tgggtcttga cgacaaggga gacgctctcg gacagcagta     3600

ccgaactgtt gaggatgtca tgtctaccgg aacggatatc ataattgtcg gccgaggtct     3660

gtacggccag aaccgagatc ctattgagga ggccaagcga taccagaagg ctggctggga     3720

ggcttaccag aagattaact gttagaggtt agactatgga tatgtaattt aactgtgtat     3780

atagagagcg tgcaagtatg gagcgcttgt tcagcttgta tgatggtcag acgacctgtc     3840

tgatcgagta tgtatgatac tgcacaacct gtgtatccgc atgatctgtc caatggggca     3900

tgttgttgtg tttctcgata cggagatgct gggtacagtg ctaatacgtt gaactactta     3960

tacttatatg aggctcgaag aaagctgact tgtgtatgac ttattctcaa ctacatcccc     4020

agtcacaata ccaccactgc actaccacta caccaaaacc atgatcaaac caccgatgga     4080

cttcctggag gcagaagaac ttgttatgga aaagctcaag agagagaatt caggagagac     4140

cgggttggcg gcgtatttgt gtcccaaaaa acagccccaa ttgccccgga gaagacggcc     4200

aggccgccta gatgacaaat tcaacaactc acagctgact ttctgccatt gccactaggg     4260

gggggccttt ttatatggcc aagccaagct ctccacgtcg gttgggctgc acccaacaat     4320

aaatgggtag ggttgcacca acaaagggat gggatggggg gtagaagata cgaggataac     4380

ggggctcaat ggcacaaata agaacgaata ctgccattaa gactcgtgat ccagcgactg     4440

acaccattgc atcatctaag ggcctcaaaa ctacctcgga actgctgcgc tgatctggac     4500

accacagagg ttccgagcac tttaggttgc accaaatgtc ccaccaggtg caggcagaaa     4560

acgctggaac agcgtgtaca gtttgtctta acaaaaagtg agggcgctga ggtcgagcag     4620

ggtggtgtga cttgttatag cctttagagc tgcgaaagcg cgtatggatt tggctcatca     4680

ggccagattg agggtctgtg gacacatgtc atgttagtgt acttcaatcg ccccctggat     4740

atagccccga caataggccg tggcctcatt tttttgcctt ccgcacattt ccattgctcg     4800

gtacccacac cttgcttctc ctgcacttgc caaccttaat actggtttac attgaccaac     4860

atcttacaag cggggggctt gtctagggta tatataaaca gtggctctcc caatcggttg     4920

ccagtctctt ttttcctttc tttccccaca gattcgaaat ctaaactaca catcacacaa     4980

tgcctgttac tgacgtcctt aagcgaaagt ccggtgtcat cgtcggcgac gatgtccgag     5040

ccgtgagtat ccacgacaag atcagtgtcg agacgacgcg ttttgtgtaa tgacacaatc     5100

cgaaagtcgc tagcaacaca cactctctac acaaactaac ccagctctcc atggctctct     5160

cccttactac cgagcagctg ctcgagcgac ccgacctggt tgccatcgac ggcattctct     5220

acgatctgga aggtcttgcc aaggtccatc ccggatccga cttgatcctc gcttctggtg     5280

cctccgatgc ttctcctctg ttctactcca tgcaccctta cgtcaagccc gagaactcga     5340

agctgcttca acagttcgtg cgaggcaagc acgaccgaac ctccaaggac attgtctaca     5400

cctacgactc tccctttgca caggacgtca agcgaactat gcgagaggtc atgaaaggtc     5460

ggaactggta tgccacacct ggattctggc tgcgaaccgt tggcatcatt gctgtcaccg     5520

ccttttgcga gtggcactgg gctactaccg gaatggtgct gtggggtctc ttgactggat     5580

tcatgcacat gcagatcggc ctgtccattc agcacgatgg ttctcatggt gccatcagca     5640

aaaagccctg ggtcaacgct ctctttgcct acggcatcga cgtcattgga tcgtccagat     5700

ggatctggct gcagtctcac atcatgcgac atcacaccta caccaatcag catggtctcg     5760

acctggatgc cgagtccgca gaaccattcc ttgtgttcca caactaccct gctgccaaca     5820

ctgctcgaaa gtggtttcac cgattccagg cctggtacat gtacctcgtg cttggagcct     5880

acggcgtttc gctggtgtac aaccctctct acatcttccg aatgcagcac aacgacacca     5940

ttcccgagtc tgtcacagcc atgcgagaga acggctttct gcgacggtac cgaacccttg     6000

cattcgttat gcgagctttc ttcatctttc gaaccgcctt cttgccctgg tatctcactg     6060

gaacctccct gctcatcacc attcctctgg tgcccactgc taccggtgcc ttcctcacct     6120

tctttttcat cttgtctcac aacttcgatg gctcggagcg aatccccgac aagaactgca     6180

aggtcaagag ctccgagaag gacgttgaag ccgatcagat cgactggtac agagctcagg     6240

tggagacctc ttccacctac ggtggaccca ttgccatgtt ctttactggc ggtctcaact     6300

tccagatcga gcatcacctc tttcctcgaa tgtcgtcttg gcactatccc ttcgtgcagc     6360

aagctgtccg agagtgttgc gaacgacacg gagttcggta cgtcttctac cctaccattg     6420

tgggcaacat catttccacc ctcaagtaca tgcacaaagt cggtgtggtt cactgtgtca     6480

aggacgctca ggattcctaa gcggccgcaa gtgtggatgg ggaagtgagt gcccggttct     6540

gtgtgcacaa ttggcaatcc aagatggatg gattcaacac agggatatag cgagctacgt     6600

ggtggtgcga ggatatagca acggatattt atgtttgaca cttgagaatg tacgatacaa     6660

gcactgtcca agtacaatac taaacatact gtacatactc atactcgtac ccgggcaacg     6720

gtttcacttg agtgcagtgg ctagtgctct tactcgtaca gtgtgcaata ctgcgtatca     6780

tagtctttga tgtatatcgt attcattcat gttagttgcg tacgacagac ggacaatgac     6840

gagccaggtt cgcccaactg acggaatctc ctatagtctt ctcatcaagc gcataaatat     6900

agtatataaa gaattacatg gtgggccaca gcttggcgat agtatgacgg tctgggctcc     6960

agatcacacc catgagggat gccgtgcgag tagatgtaga ggtggtgctt ccaggttgtc     7020

cccaacaaca atcgctactg gaaaataccg tgaaagtacg tacacccaac tctgtgactc     7080

gcgttgactt tcatctttca atttggtcgt gtcttacggt gcatgtgctg tattggacat     7140

gaagaagggg ttggcaagct gaaccatgcc tgaggtgctg gagtctgata ctgtctagca     7200

gagggcgatc aaaagatcac catccacacg tttgtttcca tattcaatct ccaatttcca     7260

acatctcctt ggctaatagc cactttgaga gatatttggt atcgcgtatt cacttgtctc     7320

atatcccgtg tttctgtttc accaaggctt ggacgatatt atcgcgagaa aaaccgtatt     7380

gaggtattta ttagagtcga gagaggagga acaggtattc ggaagatgga aaagctgtac     7440

agagcgagaa acgatcccat gtcgacgctc ctgatggctt atactgagac atggaatcag     7500

gatcttgccc ttcttcacag ctctacttgc agctacagaa ttgctttttc tcgtatccga     7560

tcataattcc gctgctatga taggcagcca ccgatacaat ataggagaga gtataggtat     7620

tgactctata gacactgtat gatgctgaca aagctgactg agggattcga ctcagcttcg     7680

ccgttgcttg atctccaatt aattatcgta ggcgcgccag ctgcattaat gaatcggcca     7740

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     7800

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     7860

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     7920

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     7980

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     8040

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     8100

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     8160

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     8220

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     8280

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     8340

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac     8400

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     8460

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     8520

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     8580

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     8640

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     8700

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     8760

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     8820

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     8880

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     8940

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     9000

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     9060

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     9120

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     9180

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     9240

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     9300

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     9360

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     9420

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     9480

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     9540

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     9600

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     9660

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa     9720

taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt     9780

gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat     9840

cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt     9900

ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt     9960

ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag    10020

gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg    10080

aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc    10140

gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc    10200

gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg    10260

cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt    10320

tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa    10380

tacgactcac tatagggcga attgggcccg acgtcgcatg cattaccaac gttggcgcgc    10440

cacaacgtca gaaactctct tccatccgat aagccgtgtc taactatacg accctcaaac    10500

aaggtcagcc agaccaactt ttagcactcg caaaacacac acccaaaccc aggccgcgac    10560

gcagccggag cccgtctact tatactgttt cgccgcagac gccaagtgac acaaaaagcg    10620

cacaaaccct cccccccagt ggcggcgcac atccacaaca aacgacccat aaacggcact    10680

cccacacgga aacaaaacgt ttgtcgggtt actcctcacg ccaagacaca ctcacacaca    10740

caacctccca cctttcatca ccactcaact acgggtacag tagtggtaga aaacacagag    10800

tccagagtcc acacagacac cgcacagagt ctgtcacaca agcaccatgc caacaagcag    10860

accgttcatt tcgctgctgt ttggaagctt ctccacgtcg tatcaacgca acaccaccac    10920

acagacctcc gcatcgacag ccaccgtcac caccggcccc acgtcgcaga tgtacaagat    10980

gctcgacaac cactcgatac ctgagcagcc ccagcagccg accctgactc agacacagcc    11040

agcggtgcag acggcgcaga cggcgcagac ggcgcagcag gcaagtggga ctgcggacct    11100

cagcgaatcg ccacacgaca agctgtggat tggaggccga tcaaacgacg gacgggaaaa    11160

gttctaccgg ctggaaccgg ccaagcgaag ggcatccttc gatcgcattt ctctggatca    11220

ggtgtctatt tgatgaggcg ccaaacagtg gccagaccaa gtctggtatc tcaacccctc    11280

aaaattgata accaagtttc tggtggagtt caaaccaagg ggagctacgg agccgttcac    11340

aaggtgtcca cagaggttca aagcacattg caccaagacc gccatcatcc ttaattaaaa    11400

ggcgttgaaa cagaatgagc cagacagcaa ggacaaggtg gccaacagca aggagtccaa    11460

aaagccctct attgacgaga tccacgatgt tattgctcat gaggtttccg agctcgatgc    11520

tgggaagaag aagtgatttg tatataagaa ataaatgaga tatagtaaag gagtgcaaga    11580

gaatggcaag gtggtcaaat tctatattac ttgcagtcac tggttcctcg ttgacatgaa    11640

tgaagttacc gttggcatag ctgatttaat atataactgt ccaactaact ctcacctaga    11700

tataacccat gtgtgtgttt ccaatcatca atgcggccgc ttaggaatcc tgtgcgtcct    11760

tcacgcagtg gacgacaccc accttatgca tgtacttcag ggtggagatg atgttgccga    11820

cgatggtagg gtagaaaaca tatcgcactc catgtcgttc gcaacactcc cggaccgcct    11880

gctggacgaa ggggtagtgc caagacgaca tccggggaaa gaggtggtgc tcgatctgga    11940

aattgagacc gccagtgaag aacatggcga tggggccacc gtatgtggag gacgtctcca    12000

cctgcgcccg ataccagtca atttggtcag cctcaacgtc cttctcagag ctcttaacct    12060

tgcagttctt gtcggggatc cgttcggagc catcaaaatt gtgggacaaa atgaagaaga    12120

acgtcaagaa ggcaccagtt gcggtgggca ccagaggaat ggtgatcagc aatgaggtcc    12180

cagtgaggta ccagggcaag aatgcggtcc ggaagatgaa gaaagctcgc atcacgaatg    12240

caagtgtgcg gtagcgccgc agaaagccat tttcccgcat ggccgtgaca gactctggga    12300

tggtgtcatt gtgctgcatc cggaaaatgt agagcgggtt gtacaccagc gataccccgt    12360

atgcccccag cacaaggtac atgtaccaag cctggaagcg gtggaaccac tttcgggcgg    12420

tgtttgcggc ggggtagttg tggaacacca ggaacggctc tgccgactcc gcatccaggt    12480

cgaggccgtg ctggttggtg taggtgtggt gccgcatgat gtgcgactgc agccaaatcc    12540

accgggacga tccgatgacg tcaatgccgt aggcgaagag ggcgttgacc caaggcttct    12600

tgctgatggc cccgtgaccc gcatcatgct ggatggataa gccgatctgc atgtgcatga    12660

atccagtcaa caggccccac agcaccatcc ccgtggtagc ccagtgccac tcgcaaaagg    12720

ccgtcacggc gatgatccca acggtgcgca gccagaagcc aggggttgcg taccagttcc    12780

tccctttcat cacctcgcgc attgtccgct taacgtcttg tgcgaaggga gaatcatacg    12840

tgtagacaat gtccttcgag gtgcggtcat gcttccctcg gacgaattgc tgcaggagct    12900

tggagttctc gggcttgacg tagggatgca tagagtagaa gaggggagag gcgtcggagg    12960

ctccagaggc cagaatgagg tctccaccac catgcacctt ggccaggccc tcgaggtcgt    13020

acaggatacc atcgatagcg acgagatcag gtcgctccag gagctgttcg gtagtgagag    13080

acagggccat ggccattgct gtagatatgt cttgtgtgta agggggttgg ggtggttgtt    13140

tgtgttcttg acttttgtgt tagcaaggga agacgggcaa aaaagtgagt gtggttggga    13200

gggagagacg agccttatat ataatgcttg tttgtgtttg tgcaagtgga cgccgaaacg    13260

ggcaggagcc aaactaaaca aggcagacaa tgcgagctta attggattgc ctgatgggca    13320

ggggttaggg ctcgatcaat gggggtgcga agtgacaaaa ttgggaatta ggttcgcaag    13380

caaggctgac aagactttgg cccaaacatt tgtacgcggt ggacaacagg agccacccat    13440

cgtctgtcac gggctagccg gtcgtgcgtc ctgtcaggct ccacctaggc tccatgccac    13500

tccatacaat cccactagtg taccgctagg ccgcttttag ctcccatcta agaccccccc    13560

aaaacctcca ctgtacagtg cactgtactg tgtggcgatc aagggcaagg gaaaaaaggc    13620

gcaaacatgc acgcatggaa tgacgtaggt aaggcgttac tagactgaaa agtggcacat    13680

ttcggcgtgc caaagggtcc taggtgcgtt tcgcgagctg ggcgccaggc caagccgctc    13740

caaaacgcct ctccgactcc ctccagcggc ctccatatcc ccatccctct ccacagcaat    13800

gttgttaagc cttgcaaacg aaaaaataga aaggctaata agcttccaat attgtggtgt    13860

acgctgcata acgcaacaat gagcgccaaa caacacacac acacagcaca cagcagcatt    13920

aaccat                                                               13926


<210>  115
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZUFmEgD9eS

<400>  115
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZUFm_6980_012208f

<400>  116
gctctggtac catggaggtc                                                   20


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZUFm_40_012208r

<400>  117
acagaaccgg gcactcactt                                                   20


<210>  118
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35F"

<400>  118
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ttc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat ggg atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  119
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  119

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  120
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZUFmEgD9eS-L35F

<400>  120
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acttctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tgggatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  121
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-K58R/I257T"

<400>  121
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg agg ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Arg Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tcg atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg caa ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

act cag tga                                                            777
Thr Gln                                                                   
                                                                          


<210>  122
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  122

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Arg Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Thr Gln 
        


<210>  123
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-K58R/I257T

<400>  123
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaggtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctcgatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc aattctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagac tcagtgagc                                       7769


<210>  124
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L130M/V243A1"

<400>  124
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct atg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aat tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gcg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  125
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  125

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  126
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-L130M/V243A1

<400>  126
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctatg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaat     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgcgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  127
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-D98G"

<400>  127
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc ggc aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Gly Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  128
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  128

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Gly Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  129
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-D98G

<400>  129
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cggcaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  130
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L130M/V243A2"

<400>  130
atg gag gtc gtg aac gaa att gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct atg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aat tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gcg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  131
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  131

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  132
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-L130M/V243A2

<400>  132
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat tgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctatg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaat     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgcgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  133
<211>  289
<212>  PRT
<213>  Ciona intestinalis

<400>  133

Met Asp Val Leu His Arg Phe Leu Gly Phe Tyr Glu Trp Thr Leu Thr 
1               5                   10                  15      


Phe Ala Asp Pro Arg Val Ala Lys Trp Pro Leu Ile Glu Asn Pro Leu 
            20                  25                  30          


Pro Thr Ile Ala Ile Val Leu Leu Tyr Leu Ala Phe Val Leu Tyr Ile 
        35                  40                  45              


Gly Pro Arg Phe Met Arg Lys Arg Ala Pro Val Asp Phe Gly Leu Phe 
    50                  55                  60                  


Leu Pro Gly Tyr Asn Phe Ala Leu Val Ala Leu Asn Tyr Tyr Ile Leu 
65                  70                  75                  80  


Gln Glu Val Val Thr Gly Ser Tyr Gly Ala Gly Tyr Asp Leu Val Cys 
                85                  90                  95      


Thr Pro Leu Arg Ser Asp Ser Tyr Asp Pro Asn Glu Met Lys Val Ala 
            100                 105                 110         


Asn Ala Val Trp Trp Tyr Tyr Val Ser Lys Ile Ile Glu Leu Phe Asp 
        115                 120                 125             


Thr Val Leu Phe Thr Leu Arg Lys Arg Asp Arg Gln Val Thr Phe Leu 
    130                 135                 140                 


His Val Tyr His His Ser Thr Met Pro Leu Leu Trp Trp Ile Gly Ala 
145                 150                 155                 160 


Lys Trp Val Pro Gly Gly Gln Ser Phe Val Gly Ile Ile Leu Asn Ser 
                165                 170                 175     


Ser Val His Val Ile Met Tyr Thr Tyr Tyr Gly Leu Ser Ala Leu Gly 
            180                 185                 190         


Pro His Met Gln Lys Phe Leu Trp Trp Lys Lys Tyr Ile Thr Met Leu 
        195                 200                 205             


Gln Leu Val Gln Phe Val Leu Ala Ile Tyr His Thr Ala Arg Ser Leu 
    210                 215                 220                 


Tyr Val Lys Cys Pro Ser Pro Val Trp Met His Trp Ala Leu Ile Leu 
225                 230                 235                 240 


Tyr Ala Phe Ser Phe Ile Leu Leu Phe Ser Asn Phe Tyr Met His Ala 
                245                 250                 255     


Tyr Ile Lys Lys Ser Arg Lys Gly Lys Glu Asn Gly Ser Arg Gly Lys 
            260                 265                 270         


Gly Gly Val Ser Asn Gly Lys Glu Lys Leu His Ala Asn Gly Lys Thr 
        275                 280                 285             


Asp 
    


<210>  134
<211>  295
<212>  PRT
<213>  Oncorhynchus mykiss

<400>  134

Met Glu Thr Phe Asn Tyr Lys Leu Asn Met Tyr Ile Asp Ser Trp Met 
1               5                   10                  15      


Gly Pro Arg Asp Glu Arg Val Gln Gly Trp Leu Leu Leu Asp Asn Tyr 
            20                  25                  30          


Pro Pro Thr Phe Ala Leu Thr Val Met Tyr Leu Leu Ile Val Trp Met 
        35                  40                  45              


Gly Pro Lys Tyr Met Arg His Arg Gln Pro Val Ser Cys Arg Gly Leu 
    50                  55                  60                  


Leu Leu Val Tyr Asn Leu Gly Leu Thr Ile Leu Ser Phe Tyr Met Phe 
65                  70                  75                  80  


Tyr Glu Met Val Ser Ala Val Trp His Gly Asp Tyr Asn Phe Phe Cys 
                85                  90                  95      


Gln Asp Thr His Ser Ala Gly Glu Thr Asp Thr Lys Ile Ile Asn Val 
            100                 105                 110         


Leu Trp Trp Tyr Tyr Phe Ser Lys Leu Ile Glu Phe Met Asp Thr Phe 
        115                 120                 125             


Phe Phe Ile Leu Arg Lys Asn Asn His Gln Ile Thr Phe Leu His Ile 
    130                 135                 140                 


Tyr His His Ala Ser Met Leu Asn Ile Trp Trp Phe Val Met Asn Trp 
145                 150                 155                 160 


Val Pro Cys Gly His Ser Tyr Phe Gly Ala Ser Leu Asn Ser Phe Ile 
                165                 170                 175     


His Val Leu Met Tyr Ser Tyr Tyr Gly Leu Ser Ala Val Pro Ala Leu 
            180                 185                 190         


Arg Pro Tyr Leu Trp Trp Lys Lys Tyr Ile Thr Gln Val Gln Leu Ile 
        195                 200                 205             


Gln Phe Phe Leu Thr Met Ser Gln Thr Ile Cys Ala Val Ile Trp Pro 
    210                 215                 220                 


Cys Asp Phe Pro Arg Gly Trp Leu Tyr Phe Gln Ile Phe Tyr Val Ile 
225                 230                 235                 240 


Thr Leu Ile Ala Leu Phe Ser Asn Phe Tyr Ile Gln Thr Tyr Lys Lys 
                245                 250                 255     


His Leu Val Ser Gln Lys Lys Glu Tyr His Gln Asn Gly Ser Val Ala 
            260                 265                 270         


Ser Leu Asn Gly His Val Asn Gly Val Thr Pro Thr Glu Thr Ile Thr 
        275                 280                 285             


His Arg Lys Val Arg Gly Asp 
    290                 295 


<210>  135
<211>  290
<212>  PRT
<213>  Marchantia polymorpha

<400>  135

Met Glu Ala Tyr Glu Met Val Asp Ser Phe Val Ser Lys Thr Val Phe 
1               5                   10                  15      


Glu Thr Leu Gln Arg Leu Arg Gly Gly Val Val Leu Thr Glu Ser Ala 
            20                  25                  30          


Ile Thr Lys Gly Leu Pro Cys Val Asp Ser Pro Thr Pro Ile Val Leu 
        35                  40                  45              


Gly Leu Ser Ser Tyr Leu Thr Phe Val Phe Leu Gly Leu Ile Val Ile 
    50                  55                  60                  


Lys Ser Leu Asp Leu Lys Pro Arg Ser Lys Glu Pro Ala Ile Leu Asn 
65                  70                  75                  80  


Leu Phe Val Ile Phe His Asn Phe Val Cys Phe Ala Leu Ser Leu Tyr 
                85                  90                  95      


Met Cys Val Gly Ile Val Arg Gln Ala Ile Leu Asn Arg Tyr Ser Leu 
            100                 105                 110         


Trp Gly Asn Ala Tyr Asn Pro Lys Glu Val Gln Met Gly His Leu Leu 
        115                 120                 125             


Tyr Ile Phe Tyr Met Ser Lys Tyr Ile Glu Phe Met Asp Thr Val Ile 
    130                 135                 140                 


Met Ile Leu Lys Arg Asn Thr Arg Gln Ile Thr Val Leu His Val Tyr 
145                 150                 155                 160 


His His Ala Ser Ile Ser Phe Ile Trp Trp Ile Ile Ala Tyr His Ala 
                165                 170                 175     


Pro Gly Gly Glu Ala Tyr Phe Ser Ala Ala Leu Asn Ser Gly Val His 
            180                 185                 190         


Val Leu Met Tyr Leu Tyr Tyr Leu Leu Ala Ala Thr Leu Gly Lys Asn 
        195                 200                 205             


Glu Lys Ala Arg Arg Lys Tyr Leu Trp Trp Gly Lys Tyr Leu Thr Gln 
    210                 215                 220                 


Leu Gln Met Phe Gln Phe Val Leu Asn Met Ile Gln Ala Tyr Tyr Asp 
225                 230                 235                 240 


Ile Lys Asn Asn Ser Pro Tyr Pro Gln Phe Leu Ile Gln Ile Leu Phe 
                245                 250                 255     


Tyr Tyr Met Ile Ser Leu Leu Ala Leu Phe Gly Asn Phe Tyr Val His 
            260                 265                 270         


Lys Tyr Val Ser Ala Pro Ala Lys Pro Ala Lys Ile Lys Ser Lys Lys 
        275                 280                 285             


Ala Glu 
    290 


<210>  136
<211>  290
<212>  PRT
<213>  Physcomitrella patens

<400>  136

Met Glu Val Val Glu Arg Phe Tyr Gly Glu Leu Asp Gly Lys Val Ser 
1               5                   10                  15      


Gln Gly Val Asn Ala Leu Leu Gly Ser Phe Gly Val Glu Leu Thr Asp 
            20                  25                  30          


Thr Pro Thr Thr Lys Gly Leu Pro Leu Val Asp Ser Pro Thr Pro Ile 
        35                  40                  45              


Val Leu Gly Val Ser Val Tyr Leu Thr Ile Val Ile Gly Gly Leu Leu 
    50                  55                  60                  


Trp Ile Lys Ala Arg Asp Leu Lys Pro Arg Ala Ser Glu Pro Phe Leu 
65                  70                  75                  80  


Leu Gln Ala Leu Val Leu Val His Asn Leu Phe Cys Phe Ala Leu Ser 
                85                  90                  95      


Leu Tyr Met Cys Val Gly Ile Ala Tyr Gln Ala Ile Thr Trp Arg Tyr 
            100                 105                 110         


Ser Leu Trp Gly Asn Ala Tyr Asn Pro Lys His Lys Glu Met Ala Ile 
        115                 120                 125             


Leu Val Tyr Leu Phe Tyr Met Ser Lys Tyr Val Glu Phe Met Asp Thr 
    130                 135                 140                 


Val Ile Met Ile Leu Lys Arg Ser Thr Arg Gln Ile Ser Phe Leu His 
145                 150                 155                 160 


Val Tyr His His Ser Ser Ile Ser Leu Ile Trp Trp Ala Ile Ala His 
                165                 170                 175     


His Ala Pro Gly Gly Glu Ala Tyr Trp Ser Ala Ala Leu Asn Ser Gly 
            180                 185                 190         


Val His Val Leu Met Tyr Ala Tyr Tyr Phe Leu Ala Ala Cys Leu Arg 
        195                 200                 205             


Ser Ser Pro Lys Leu Lys Asn Lys Tyr Leu Phe Trp Gly Arg Tyr Leu 
    210                 215                 220                 


Thr Gln Phe Gln Met Phe Gln Phe Met Leu Asn Leu Val Gln Ala Tyr 
225                 230                 235                 240 


Tyr Asp Met Lys Thr Asn Ala Pro Tyr Pro Gln Trp Leu Ile Lys Ile 
                245                 250                 255     


Leu Phe Tyr Tyr Met Ile Ser Leu Leu Phe Leu Phe Gly Asn Phe Tyr 
            260                 265                 270         


Val Gln Lys Tyr Ile Lys Pro Ser Asp Gly Lys Gln Lys Gly Ala Lys 
        275                 280                 285             


Thr Glu 
    290 


<210>  137
<211>  348
<212>  PRT
<213>  Marchantia polymorpha

<400>  137

Met Ala Thr Lys Ser Gly Ser Gly Leu Leu Glu Trp Ile Ala Val Ala 
1               5                   10                  15      


Ala Lys Met Lys Gln Ala Arg Ser Ser Pro Glu Gly Glu Ile Val Gly 
            20                  25                  30          


Gly Asn Arg Met Gly Ser Gly Asn Gly Ala Glu Trp Thr Thr Ser Leu 
        35                  40                  45              


Ile His Ala Phe Leu Asn Ala Thr Asn Gly Lys Ser Gly Gly Ala Ser 
    50                  55                  60                  


Lys Val Arg Pro Leu Glu Glu Arg Ile Gly Glu Ala Val Phe Arg Val 
65                  70                  75                  80  


Leu Glu Asp Val Val Gly Val Asp Ile Arg Lys Pro Asn Pro Val Thr 
                85                  90                  95      


Lys Asp Leu Pro Met Val Glu Ser Pro Val Pro Val Leu Ala Cys Ile 
            100                 105                 110         


Ser Leu Tyr Leu Leu Val Val Trp Leu Trp Ser Ser His Ile Lys Ala 
        115                 120                 125             


Ser Gly Gln Lys Pro Arg Lys Glu Asp Pro Leu Ala Leu Arg Cys Leu 
    130                 135                 140                 


Val Ile Ala His Asn Leu Phe Leu Cys Cys Leu Ser Leu Phe Met Cys 
145                 150                 155                 160 


Val Gly Leu Ile Ala Ala Ala Arg His Tyr Gly Tyr Ser Val Trp Gly 
                165                 170                 175     


Asn Tyr Tyr Arg Glu Arg Glu Pro Ala Met Asn Leu Leu Ile Tyr Val 
            180                 185                 190         


Phe Tyr Met Ser Lys Leu Tyr Glu Phe Met Asp Thr Ala Ile Met Leu 
        195                 200                 205             


Phe Arg Arg Asn Leu Arg Gln Val Thr Tyr Leu His Val Tyr His His 
    210                 215                 220                 


Ala Ser Ile Ala Met Ile Trp Trp Ile Ile Cys Tyr Arg Phe Pro Gly 
225                 230                 235                 240 


Ala Asp Ser Tyr Phe Ser Ala Ala Phe Asn Ser Cys Ile His Val Ala 
                245                 250                 255     


Met Tyr Leu Tyr Tyr Leu Leu Ala Ala Thr Val Ala Arg Asp Glu Lys 
            260                 265                 270         


Arg Arg Arg Lys Tyr Leu Phe Trp Gly Lys Tyr Leu Thr Ile Ile Gln 
        275                 280                 285             


Met Leu Gln Phe Leu Ser Phe Ile Gly Gln Ala Ile Tyr Ala Met Trp 
    290                 295                 300                 


Lys Phe Glu Tyr Tyr Pro Lys Gly Phe Gly Arg Met Leu Phe Phe Tyr 
305                 310                 315                 320 


Ser Val Ser Leu Leu Ala Phe Phe Gly Asn Phe Phe Val Lys Lys Tyr 
                325                 330                 335     


Ser Asn Ala Ser Gln Pro Lys Thr Val Lys Val Glu 
            340                 345             


<210>  138
<211>  292
<212>  PRT
<213>  Ostreococcus tauri

<400>  138

Met Ser Gly Leu Arg Ala Pro Asn Phe Leu His Arg Phe Trp Thr Lys 
1               5                   10                  15      


Trp Asp Tyr Ala Ile Ser Lys Val Val Phe Thr Cys Ala Asp Ser Phe 
            20                  25                  30          


Gln Trp Asp Ile Gly Pro Val Ser Ser Ser Thr Ala His Leu Pro Ala 
        35                  40                  45              


Ile Glu Ser Pro Thr Pro Leu Val Thr Ser Leu Leu Phe Tyr Leu Val 
    50                  55                  60                  


Thr Val Phe Leu Trp Tyr Gly Arg Leu Thr Arg Ser Ser Asp Lys Lys 
65                  70                  75                  80  


Ile Arg Glu Pro Thr Trp Leu Arg Arg Phe Ile Ile Cys His Asn Ala 
                85                  90                  95      


Phe Leu Ile Val Leu Ser Leu Tyr Met Cys Leu Gly Cys Val Ala Gln 
            100                 105                 110         


Ala Tyr Gln Asn Gly Tyr Thr Leu Trp Gly Asn Glu Phe Lys Ala Thr 
        115                 120                 125             


Glu Thr Gln Leu Ala Leu Tyr Ile Tyr Ile Phe Tyr Val Ser Lys Ile 
    130                 135                 140                 


Tyr Glu Phe Val Asp Thr Tyr Ile Met Leu Leu Lys Asn Asn Leu Arg 
145                 150                 155                 160 


Gln Val Ser Phe Leu His Ile Tyr His His Ser Thr Ile Ser Phe Ile 
                165                 170                 175     


Trp Trp Ile Ile Ala Arg Arg Ala Pro Gly Gly Asp Ala Tyr Phe Ser 
            180                 185                 190         


Ala Ala Leu Asn Ser Trp Val His Val Cys Met Tyr Thr Tyr Tyr Leu 
        195                 200                 205             


Leu Ser Thr Leu Ile Gly Lys Glu Asp Pro Lys Arg Ser Asn Tyr Leu 
    210                 215                 220                 


Trp Trp Gly Arg His Leu Thr Gln Met Gln Met Leu Gln Phe Phe Phe 
225                 230                 235                 240 


Asn Val Leu Gln Ala Leu Tyr Cys Ala Ser Phe Ser Thr Tyr Pro Lys 
                245                 250                 255     


Phe Leu Ser Lys Ile Leu Leu Val Tyr Met Met Ser Leu Leu Gly Leu 
            260                 265                 270         


Phe Gly His Phe Tyr Tyr Ser Lys His Ile Ala Ala Ala Lys Leu Gln 
        275                 280                 285             


Lys Lys Gln Gln 
    290         


<210>  139
<211>  277
<212>  PRT
<213>  Pavlova sp.

<400>  139

Met Met Leu Ala Ala Gly Tyr Leu Leu Val Leu Ser Ala Ala Arg Gln 
1               5                   10                  15      


Ser Phe Gln Gln Asp Ile Asp Asn Pro Asn Gly Ala Tyr Ser Thr Ser 
            20                  25                  30          


Trp Thr Gly Leu Pro Ile Val Met Ser Val Val Tyr Leu Ser Gly Val 
        35                  40                  45              


Phe Gly Leu Thr Lys Tyr Phe Glu Asn Arg Lys Pro Met Thr Gly Leu 
    50                  55                  60                  


Lys Asp Tyr Met Phe Thr Tyr Asn Leu Tyr Gln Val Ile Ile Asn Val 
65                  70                  75                  80  


Trp Cys Val Val Ala Phe Leu Leu Glu Val Arg Arg Ala Gly Met Ser 
                85                  90                  95      


Leu Ile Gly Asn Lys Val Asp Leu Gly Pro Asn Ser Phe Arg Leu Gly 
            100                 105                 110         


Phe Val Thr Trp Val His Tyr Asn Asn Lys Tyr Val Glu Leu Leu Asp 
        115                 120                 125             


Thr Leu Trp Met Val Leu Arg Lys Lys Thr Gln Gln Val Ser Phe Leu 
    130                 135                 140                 


His Val Tyr His His Val Leu Leu Met Trp Ala Trp Phe Val Val Val 
145                 150                 155                 160 


Lys Leu Gly Asn Gly Gly Asp Ala Tyr Phe Gly Gly Leu Met Asn Ser 
                165                 170                 175     


Ile Ile His Val Met Met Tyr Ser Tyr Tyr Thr Met Ala Leu Leu Gly 
            180                 185                 190         


Trp Ser Cys Pro Trp Lys Arg Tyr Leu Thr Gln Ala Gln Leu Val Gln 
        195                 200                 205             


Phe Cys Ile Cys Leu Ala His Ser Thr Trp Ala Ala Val Thr Gly Ala 
    210                 215                 220                 


Tyr Pro Trp Arg Ile Cys Leu Val Glu Val Trp Val Met Val Ser Met 
225                 230                 235                 240 


Leu Val Leu Phe Thr Arg Phe Tyr Arg Gln Ala Tyr Ala Lys Glu Ala 
                245                 250                 255     


Lys Ala Lys Glu Ala Lys Lys Leu Ala Gln Glu Ala Ser Gln Ala Lys 
            260                 265                 270         


Ala Val Lys Ala Glu 
        275         


<210>  140
<211>  302
<212>  PRT
<213>  Rebecca salina

<400>  140

Met Lys Ala Ala Ala Gly Lys Val Gln Gln Glu Ala Glu Arg Leu Thr 
1               5                   10                  15      


Ala Gly Leu Trp Leu Pro Met Met Leu Ala Ala Gly Tyr Leu Leu Val 
            20                  25                  30          


Leu Ser Ala Asn Arg Ala Ser Phe Tyr Glu Asn Ile Asn Asn Glu Lys 
        35                  40                  45              


Gly Ala Tyr Ser Thr Ser Trp Phe Ser Leu Pro Cys Val Met Thr Ala 
    50                  55                  60                  


Val Tyr Leu Gly Gly Val Phe Gly Leu Thr Lys Tyr Phe Glu Gly Arg 
65                  70                  75                  80  


Lys Pro Met Gln Gly Leu Lys Asp Tyr Met Phe Thr Tyr Asn Leu Tyr 
                85                  90                  95      


Gln Val Ile Ile Asn Val Trp Cys Ile Ala Ala Phe Val Val Glu Val 
            100                 105                 110         


Arg Arg Ala Gly Met Ser Ala Val Gly Asn Lys Val Asp Leu Gly Pro 
        115                 120                 125             


Asn Ser Phe Arg Leu Gly Phe Val Thr Trp Val His Tyr Asn Asn Lys 
    130                 135                 140                 


Tyr Val Glu Leu Leu Asp Thr Leu Trp Met Val Leu Arg Lys Lys Thr 
145                 150                 155                 160 


Gln Gln Val Ser Phe Leu His Val Tyr His His Val Leu Leu Ile Trp 
                165                 170                 175     


Ala Trp Phe Cys Val Val Lys Phe Cys Asn Gly Gly Asp Ala Tyr Phe 
            180                 185                 190         


Gly Gly Met Leu Asn Ser Ile Ile His Val Met Met Tyr Ser Tyr Tyr 
        195                 200                 205             


Thr Met Ala Leu Leu Gly Trp Ser Cys Pro Trp Lys Arg Tyr Leu Thr 
    210                 215                 220                 


Gln Ala Gln Leu Val Gln Phe Cys Ile Cys Leu Ala His Ala Thr Trp 
225                 230                 235                 240 


Ala Ala Ala Thr Gly Val Tyr Pro Phe His Ile Cys Leu Val Glu Ile 
                245                 250                 255     


Trp Val Met Val Ser Met Leu Tyr Leu Phe Thr Lys Phe Tyr Asn Ser 
            260                 265                 270         


Ala Tyr Lys Gly Ala Ala Lys Gly Ala Ala Ala Ser Ser Asn Gly Ala 
        275                 280                 285             


Ala Ala Pro Ser Gly Ala Lys Pro Lys Ser Ile Lys Ala Asn 
    290                 295                 300         


<210>  141
<211>  300
<212>  PRT
<213>  Ostreococcus tauri

<400>  141

Met Ser Ala Ser Gly Ala Leu Leu Pro Ala Ile Ala Ser Ala Ala Tyr 
1               5                   10                  15      


Ala Tyr Ala Thr Tyr Ala Tyr Ala Phe Glu Trp Ser His Ala Asn Gly 
            20                  25                  30          


Ile Asp Asn Val Asp Ala Arg Glu Trp Ile Gly Ala Leu Ser Leu Arg 
        35                  40                  45              


Leu Pro Ala Ile Ala Thr Thr Met Tyr Leu Leu Phe Cys Leu Val Gly 
    50                  55                  60                  


Pro Arg Leu Met Ala Lys Arg Glu Ala Phe Asp Pro Lys Gly Phe Met 
65                  70                  75                  80  


Leu Ala Tyr Asn Ala Tyr Gln Thr Ala Phe Asn Val Val Val Leu Gly 
                85                  90                  95      


Met Phe Ala Arg Glu Ile Ser Gly Leu Gly Gln Pro Val Trp Gly Ser 
            100                 105                 110         


Thr Met Pro Trp Ser Asp Arg Lys Ser Phe Lys Ile Leu Leu Gly Val 
        115                 120                 125             


Trp Leu His Tyr Asn Asn Lys Tyr Leu Glu Leu Leu Asp Thr Val Phe 
    130                 135                 140                 


Met Val Ala Arg Lys Lys Thr Lys Gln Leu Ser Phe Leu His Val Tyr 
145                 150                 155                 160 


His His Ala Leu Leu Ile Trp Ala Trp Trp Leu Val Cys His Leu Met 
                165                 170                 175     


Ala Thr Asn Asp Cys Ile Asp Ala Tyr Phe Gly Ala Ala Cys Asn Ser 
            180                 185                 190         


Phe Ile His Ile Val Met Tyr Ser Tyr Tyr Leu Met Ser Ala Leu Gly 
        195                 200                 205             


Ile Arg Cys Pro Trp Lys Arg Tyr Ile Thr Gln Ala Gln Met Leu Gln 
    210                 215                 220                 


Phe Val Ile Val Phe Ala His Ala Val Phe Val Leu Arg Gln Lys His 
225                 230                 235                 240 


Cys Pro Val Thr Leu Pro Trp Ala Gln Met Phe Val Met Thr Asn Met 
                245                 250                 255     


Leu Val Leu Phe Gly Asn Phe Tyr Leu Lys Ala Tyr Ser Asn Lys Ser 
            260                 265                 270         


Arg Gly Asp Gly Ala Ser Ser Val Lys Pro Ala Glu Thr Thr Arg Ala 
        275                 280                 285             


Pro Ser Val Arg Arg Thr Arg Ser Arg Lys Ile Asp 
    290                 295                 300 


<210>  142
<211>  358
<212>  PRT
<213>  Thalassiosira pseudonana

<400>  142

Met Cys Ser Ser Pro Pro Ser Gln Ser Lys Thr Thr Ser Leu Leu Ala 
1               5                   10                  15      


Arg Tyr Thr Thr Ala Ala Leu Leu Leu Leu Thr Leu Thr Thr Trp Cys 
            20                  25                  30          


His Phe Ala Phe Pro Ala Ala Thr Ala Thr Pro Gly Leu Thr Ala Glu 
        35                  40                  45              


Met His Ser Tyr Lys Val Pro Leu Gly Leu Thr Val Phe Tyr Leu Leu 
    50                  55                  60                  


Ser Leu Pro Ser Leu Lys Tyr Val Thr Asp Asn Tyr Leu Ala Lys Lys 
65                  70                  75                  80  


Tyr Asp Met Lys Ser Leu Leu Thr Glu Ser Met Val Leu Tyr Asn Val 
                85                  90                  95      


Ala Gln Val Leu Leu Asn Gly Trp Thr Val Tyr Ala Ile Val Asp Ala 
            100                 105                 110         


Val Met Asn Arg Asp His Pro Phe Ile Gly Ser Arg Ser Leu Val Gly 
        115                 120                 125             


Ala Ala Leu His Ser Gly Ser Ser Tyr Ala Val Trp Val His Tyr Cys 
    130                 135                 140                 


Asp Lys Tyr Leu Glu Phe Phe Asp Thr Tyr Phe Met Val Leu Arg Gly 
145                 150                 155                 160 


Lys Met Asp Gln Val Ser Phe Leu His Ile Tyr His His Thr Thr Ile 
                165                 170                 175     


Ala Trp Ala Trp Trp Ile Ala Leu Arg Phe Ser Pro Gly Gly Asp Ile 
            180                 185                 190         


Tyr Phe Gly Ala Leu Leu Asn Ser Ile Ile His Val Leu Met Tyr Ser 
        195                 200                 205             


Tyr Tyr Ala Leu Ala Leu Leu Lys Val Ser Cys Pro Trp Lys Arg Tyr 
    210                 215                 220                 


Leu Thr Gln Ala Gln Leu Leu Gln Phe Thr Ser Val Val Val Tyr Thr 
225                 230                 235                 240 


Gly Cys Thr Gly Tyr Thr His Tyr Tyr His Thr Lys His Gly Ala Asp 
                245                 250                 255     


Glu Thr Gln Pro Ser Leu Gly Thr Tyr Tyr Phe Cys Cys Gly Val Gln 
            260                 265                 270         


Val Phe Glu Met Val Ser Leu Phe Val Leu Phe Ser Ile Phe Tyr Lys 
        275                 280                 285             


Arg Ser Tyr Ser Lys Lys Asn Lys Ser Gly Gly Lys Asp Ser Lys Lys 
    290                 295                 300                 


Asn Asp Asp Gly Asn Asn Glu Asp Gln Cys His Lys Ala Met Lys Asp 
305                 310                 315                 320 


Ile Ser Glu Gly Ala Lys Glu Val Val Gly His Ala Ala Lys Asp Ala 
                325                 330                 335     


Gly Lys Leu Val Ala Thr Ala Ser Lys Ala Val Lys Arg Lys Gly Thr 
            340                 345                 350         


Arg Val Thr Gly Ala Met 
        355             


<210>  143
<211>  272
<212>  PRT
<213>  Thalassiosira pseudonana

<400>  143

Met Asp Ala Tyr Asn Ala Ala Met Asp Lys Ile Gly Ala Ala Ile Ile 
1               5                   10                  15      


Asp Trp Ser Asp Pro Asp Gly Lys Phe Arg Ala Asp Arg Glu Asp Trp 
            20                  25                  30          


Trp Leu Cys Asp Phe Arg Ser Ala Ile Thr Ile Ala Leu Ile Tyr Ile 
        35                  40                  45              


Ala Phe Val Ile Leu Gly Ser Ala Val Met Gln Ser Leu Pro Ala Met 
    50                  55                  60                  


Asp Pro Tyr Pro Ile Lys Phe Leu Tyr Asn Val Ser Gln Ile Phe Leu 
65                  70                  75                  80  


Cys Ala Tyr Met Thr Val Glu Ala Gly Phe Leu Ala Tyr Arg Asn Gly 
                85                  90                  95      


Tyr Thr Val Met Pro Cys Asn His Phe Asn Val Asn Asp Pro Pro Val 
            100                 105                 110         


Ala Asn Leu Leu Trp Leu Phe Tyr Ile Ser Lys Val Trp Asp Phe Trp 
        115                 120                 125             


Asp Thr Ile Phe Ile Val Leu Gly Lys Lys Trp Arg Gln Leu Ser Phe 
    130                 135                 140                 


Leu His Val Tyr His His Thr Thr Ile Phe Leu Phe Tyr Trp Leu Asn 
145                 150                 155                 160 


Ala Asn Val Leu Tyr Asp Gly Asp Ile Phe Leu Thr Ile Leu Leu Asn 
                165                 170                 175     


Gly Phe Ile His Thr Val Met Tyr Thr Tyr Tyr Phe Ile Cys Met His 
            180                 185                 190         


Thr Lys Asp Ser Lys Thr Gly Lys Ser Leu Pro Ile Trp Trp Lys Ser 
        195                 200                 205             


Ser Leu Thr Ala Phe Gln Leu Leu Gln Phe Thr Ile Met Met Ser Gln 
    210                 215                 220                 


Ala Thr Tyr Leu Val Phe His Gly Cys Asp Lys Val Ser Leu Arg Ile 
225                 230                 235                 240 


Thr Ile Val Tyr Phe Val Ser Leu Leu Ser Leu Phe Phe Leu Phe Ala 
                245                 250                 255     


Gln Phe Phe Val Gln Ser Tyr Met Ala Pro Lys Lys Lys Lys Ser Ala 
            260                 265                 270         


<210>  144
<211>  318
<212>  PRT
<213>  Mortierella alpina

<400>  144

Met Glu Ser Ile Ala Pro Phe Leu Pro Ser Lys Met Pro Gln Asp Leu 
1               5                   10                  15      


Phe Met Asp Leu Ala Thr Ala Ile Gly Val Arg Ala Ala Pro Tyr Val 
            20                  25                  30          


Asp Pro Leu Glu Ala Ala Leu Val Ala Gln Ala Glu Lys Tyr Ile Pro 
        35                  40                  45              


Thr Ile Val His His Thr Arg Gly Phe Leu Val Ala Val Glu Ser Pro 
    50                  55                  60                  


Leu Ala Arg Glu Leu Pro Leu Met Asn Pro Phe His Val Leu Leu Ile 
65                  70                  75                  80  


Val Leu Ala Tyr Leu Val Thr Val Phe Val Gly Met Gln Ile Met Lys 
                85                  90                  95      


Asn Phe Glu Arg Phe Glu Val Lys Thr Phe Ser Leu Leu His Asn Phe 
            100                 105                 110         


Cys Leu Val Ser Ile Ser Ala Tyr Met Cys Gly Gly Ile Leu Tyr Glu 
        115                 120                 125             


Ala Tyr Gln Ala Asn Tyr Gly Leu Phe Glu Asn Ala Ala Asp His Thr 
    130                 135                 140                 


Phe Lys Gly Leu Pro Met Ala Lys Met Ile Trp Leu Phe Tyr Phe Ser 
145                 150                 155                 160 


Lys Ile Met Glu Phe Val Asp Thr Met Ile Met Val Leu Lys Lys Asn 
                165                 170                 175     


Asn Arg Gln Ile Ser Phe Leu His Val Tyr His His Ser Ser Ile Phe 
            180                 185                 190         


Thr Ile Trp Trp Leu Val Thr Phe Val Ala Pro Asn Gly Glu Ala Tyr 
        195                 200                 205             


Phe Ser Ala Ala Leu Asn Ser Phe Ile His Val Ile Met Tyr Gly Tyr 
    210                 215                 220                 


Tyr Phe Leu Ser Ala Leu Gly Phe Lys Gln Val Ser Phe Ile Lys Phe 
225                 230                 235                 240 


Tyr Ile Thr Arg Ser Gln Met Thr Gln Phe Cys Met Met Ser Val Gln 
                245                 250                 255     


Ser Ser Trp Asp Met Tyr Ala Met Lys Val Leu Gly Arg Pro Gly Tyr 
            260                 265                 270         


Pro Phe Phe Ile Thr Ala Leu Leu Trp Phe Tyr Met Trp Thr Met Leu 
        275                 280                 285             


Gly Leu Phe Tyr Asn Phe Tyr Arg Lys Asn Ala Lys Leu Ala Lys Gln 
    290                 295                 300                 


Ala Lys Ala Asp Ala Ala Lys Glu Lys Ala Arg Lys Leu Gln 
305                 310                 315             


<210>  145
<211>  271
<212>  PRT
<213>  Thraustochytrium sp.

<400>  145

Met Met Glu Pro Leu Asp Arg Tyr Arg Ala Leu Ala Glu Leu Ala Ala 
1               5                   10                  15      


Arg Tyr Ala Ser Ser Ala Ala Phe Lys Trp Gln Val Thr Tyr Asp Ala 
            20                  25                  30          


Lys Asp Ser Phe Val Gly Pro Leu Gly Ile Arg Glu Pro Leu Gly Leu 
        35                  40                  45              


Leu Val Gly Ser Val Val Leu Tyr Leu Ser Leu Gln Ala Val Val Tyr 
    50                  55                  60                  


Ala Leu Arg Asn Tyr Leu Gly Gly Leu Met Ala Leu Arg Ser Val His 
65                  70                  75                  80  


Asn Leu Gly Leu Cys Leu Phe Ser Gly Ala Val Trp Ile Tyr Thr Ser 
                85                  90                  95      


Tyr Leu Met Ile Gln Asp Gly His Phe Arg Ser Leu Glu Ala Ala Thr 
            100                 105                 110         


Cys Glu Pro Leu Lys His Pro His Phe Gln Leu Ile Ser Leu Leu Phe 
        115                 120                 125             


Ala Leu Ser Lys Ile Trp Glu Trp Phe Asp Thr Val Leu Leu Ile Val 
    130                 135                 140                 


Lys Gly Asn Lys Leu Arg Phe Leu His Val Leu His His Ala Thr Thr 
145                 150                 155                 160 


Phe Trp Leu Tyr Ala Ile Asp His Ile Phe Leu Ser Ser Ile Lys Tyr 
                165                 170                 175     


Gly Val Ala Val Asn Ala Phe Ile His Thr Val Met Tyr Ala His Tyr 
            180                 185                 190         


Phe Arg Pro Phe Pro Lys Gly Leu Arg Pro Leu Ile Thr Gln Leu Gln 
        195                 200                 205             


Ile Val Gln Phe Ile Phe Ser Ile Gly Ile His Thr Ala Ile Tyr Trp 
    210                 215                 220                 


His Tyr Asp Cys Glu Pro Leu Val His Thr His Phe Trp Glu Tyr Val 
225                 230                 235                 240 


Thr Pro Tyr Leu Phe Val Val Pro Phe Leu Ile Leu Phe Leu Asn Phe 
                245                 250                 255     


Tyr Leu Gln Gln Tyr Val Leu Ala Pro Ala Lys Thr Lys Lys Ala 
            260                 265                 270     


<210>  146
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EgD9E_102_053008f


<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  n is a, c, g, or t

<400>  146
gtgctgtacn nktccatcgc ctt                                               23


<210>  147
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EgD9E_760_053008r

<400>  147
tcccttgtgc tttcggacga tgta                                              24


<210>  148
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9ES-L35G

<400>  148
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  149
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35M/Q107E"

<400>  149
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac atg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Met Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act gag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Glu Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  150
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  150

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Met Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Glu Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  151
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9ES-L35M/Q107E

<400>  151
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acatgtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactga gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  152
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  152
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat       60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acggatccat cgccttcgtc      120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg      180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc      240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat      300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc      360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga      420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg      480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag      540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt      600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga      660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac      720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga         777


<210>  153
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  153
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat       60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acggctccat cgccttcgtc      120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg      180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc      240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat      300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc      360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga      420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg      480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag      540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt      600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga      660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac      720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga         777


<210>  154
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  154
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat       60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acggttccat cgccttcgtc      120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg      180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc      240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat      300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc      360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga      420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg      480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag      540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt      600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga      660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac      720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga         777


<210>  155
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_9D_122709f

<400>  155
gaaatcgtcg acattggcca gg                                                22


<210>  156
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_84C_122709r

<400>  156
gacaccaatg gtacacatgg cgtaggc                                           27


<210>  157
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_84C_122709f

<400>  157
gcctacgcca tgtgtaccat tggtgtc                                           27


<210>  158
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_9D_122709r

<400>  158
cctggccaat gtcgacgatt tc                                                22


<210>  159
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_161T_122709f

<400>  159
gatctttgtg accctcaacg gcttc                                             25


<210>  160
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_179R_122709r

<400>  160
gaacttgagc tttcgcagtc gggtc                                             25


<210>  161
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_179R_122709f

<400>  161
gacccgactg cgaaagctca agttc                                             25


<210>  162
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_161T_122709r

<400>  162
gaagccgttg agggtcacaa agatc                                             25


<210>  163
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_244N_122709f

<400>  163
cttctacgtg aacacctaca tc                                                22


<210>  164
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_21V_010710r

<400>  164
gaccagagct gcacatagtc gac                                               23


<210>  165
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_21V_010710f

<400>  165
gtcgactatg tgcagctctg gtc                                               23


<210>  166
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_244N_122709r

<400>  166
gatgtaggtg ttcacgtaga ag                                                22


<210>  167
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_32F_010710f

<400>  167
gcactgcgag tttctgtacc tc                                                22


<210>  168
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_108G_010710r

<400>  168
gaggtagaaa ccctgagtgg tg                                                22


<210>  169
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_108G_010710f

<400>  169
caccactcag ggtttctacc tc                                                22


<210>  170
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_32F_010710r

<400>  170
gaggtacaga aactcgcagt gc                                                22


<210>  171
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_132T_010710f

<400>  171
gcctctgacc accttgcagt tc                                                22


<210>  172
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_143N_010710r

<400>  172
gccacatgtc gttaggagct cc                                                22


<210>  173
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_143N_010710f

<400>  173
ggagctccta acgacatgtg gc                                                22


<210>  174
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_132T_010710r

<400>  174
gaactgcaag gtggtcagag gc                                                22


<210>  175
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_236N_010710f

<400>  175
gtactgtcct gaacctgttc ctc                                               23


<210>  176
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_236N_010710r

<400>  176
gaggaacagg ttcaggacag tac                                               23


<210>  177
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-A21V/L35G/L108G/I179R"

<400>  177
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gtg cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ggt ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Gly Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  178
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  178

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Gly Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  179
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9ES-A21V/L35G/L108G/I179R

<400>  179
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gtgcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gggtttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  180
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HXGX motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  180

His Xaa Gly Xaa 
1               


<210>  181
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPGG motif

<400>  181

His Pro Gly Gly 
1               


<210>  182
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HXXXH motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  182

His Xaa Xaa Xaa His 
1               5   


<210>  183
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDASH motif

<400>  183

His Asp Ala Ser His 
1               5   


<210>  184
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,678,560
<311>  2007-05-15
<312>  2010-03-16
<313>  (1)..(1350)

<400>  184
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aat tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc caa gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc act gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag agc tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cgc cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc tga                                                               1350
Ser                                                                       
                                                                          


<210>  185
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  185

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  186
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  186

His Xaa Xaa Xaa His 
1               5   


<210>  187
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  187

His Xaa Xaa Xaa Xaa His 
1               5       


<210>  188
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  188

His Xaa Xaa His His 
1               5   


<210>  189
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  189

His Xaa Xaa Xaa His His 
1               5       


<210>  190
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = Gln [Q] or His [H]

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  190

Xaa Xaa Xaa His His 
1               5   


<210>  191
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-rich motif


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = Gln [Q] or His [H]

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  191

Xaa Xaa Xaa Xaa His His 
1               5       


<210>  192
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase ("EgD5R")

<400>  192
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aat tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc caa gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag aga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cgc cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  193
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  193

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  194
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367

<400>  194
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga       60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct      120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc      180

ggagaattcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga      240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt      300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat      360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct      420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg cgtcccacgg      480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg      540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca      600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc      660

cgccgcaaac accgcccgaa agtggttcca ccgcttccaa gcttggtaca tgtaccttgt      720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca      780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta      840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg      900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc      960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga     1020

caagaactgc aaggttaaga gatctgagaa ggacgttgag gctgaccaaa ttgactggta     1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg     1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc     1200

cttcgtccag caggcggtcc gggagtgttg cgaacgccat ggagtgcgat atgttttcta     1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt     1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag     1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata     1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat     1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta     1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat     1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg     1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct     3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga     4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag     4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg     4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg     4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact     4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta     4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt     4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg     4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt     4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa     4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt     5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc     5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca     5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa     5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc     5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata     5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc     5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt     5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag     5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga     5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga     5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac     5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata     5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg     5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta     5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt     5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag     6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac     6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt     6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc     6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca     6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga     6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg     6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc     6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc     6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac     6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca     6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg     6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg     6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc     6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca     6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa     6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac     6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa     7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta     7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc     7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca     7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac     7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac     7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact     7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg     7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag     7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt     7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg     7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg     7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca     7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt     7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca     7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac     7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga     7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac     8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc     8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc     8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt     8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact     8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc     8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct     8400

agcaacacac actctctaca caaactaacc cagctctc                             8438


<210>  195
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/136671
<311>  2007-05-16
<312>  2007-11-29
<313>  (1)..(1350)

<300>
<302>  DELTA-5 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 2007-0292924-A1
<311>  2007-05-15
<312>  2007-12-20
<313>  (1)..(1350)

<400>  195
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga ggc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc tct cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  196
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  196

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  197
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)
<223>  delta-5 desaturase ("EgD5R*")

<400>  197
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat cca gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aac tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg tcc cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag cga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  198
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  198

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  199
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367-M4

<400>  199
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga       60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct      120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc      180

ggagaactcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga      240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt      300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat      360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct      420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg cgtcccacgg      480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg      540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca      600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc      660

cgccgcaaac accgcccgaa agtggttcca ccgcttccag gcttggtaca tgtaccttgt      720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca      780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta      840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg      900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc      960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga     1020

caagaactgc aaggttaagc gatctgagaa ggacgttgag gctgaccaaa ttgactggta     1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg     1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc     1200

cttcgtccag caggcggtcc gggagtgttg cgaacgacat ggagtgcgat atgttttcta     1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt     1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag     1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata     1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat     1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta     1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat     1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg     1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct     3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga     4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag     4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg     4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg     4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact     4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta     4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt     4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg     4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt     4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa     4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt     5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc     5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca     5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa     5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc     5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata     5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc     5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt     5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag     5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga     5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga     5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac     5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata     5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg     5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta     5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt     5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag     6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac     6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt     6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc     6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca     6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga     6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg     6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc     6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc     6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac     6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca     6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg     6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg     6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc     6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca     6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa     6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac     6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa     7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta     7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc     7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca     7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac     7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac     7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact     7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg     7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag     7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt     7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg     7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg     7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca     7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt     7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca     7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac     7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga     7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac     8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc     8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc     8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt     8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact     8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc     8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct     8400

agcaacacac actctctaca caaactaacc cagctctc                             8438


<210>  200
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 813

<400>  200
cgtcaaaccg gagaactcca aattgcttca a                                      31


<210>  201
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 814

<400>  201
ttgaagcaat ttggagttct ccggtttgac g                                      31


<210>  202
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 815

<400>  202
aactgcaagg ttaagcgatc tgagaaggac g                                      31


<210>  203
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 816

<400>  203
cgtccttctc agatcgctta accttgcagt t                                      31


<210>  204
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367-M2

<400>  204
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga       60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct      120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc      180

ggagaactcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga      240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt      300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat      360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct      420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg cgtcccacgg      480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg      540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca      600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc      660

cgccgcaaac accgcccgaa agtggttcca ccgcttccaa gcttggtaca tgtaccttgt      720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca      780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta      840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg      900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc      960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga     1020

caagaactgc aaggttaagc gatctgagaa ggacgttgag gctgaccaaa ttgactggta     1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg     1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc     1200

cttcgtccag caggcggtcc gggagtgttg cgaacgccat ggagtgcgat atgttttcta     1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt     1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag     1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata     1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat     1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta     1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat     1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg     1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct     3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga     4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag     4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg     4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg     4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact     4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta     4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt     4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg     4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt     4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa     4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt     5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc     5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca     5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa     5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc     5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata     5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc     5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt     5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag     5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga     5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga     5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac     5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata     5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg     5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta     5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt     5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag     6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac     6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt     6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc     6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca     6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga     6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg     6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc     6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc     6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac     6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca     6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg     6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg     6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc     6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca     6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa     6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac     6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa     7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta     7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc     7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca     7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac     7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac     7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact     7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg     7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag     7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt     7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg     7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg     7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca     7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt     7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca     7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac     7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga     7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac     8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc     8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc     8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt     8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact     8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc     8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct     8400

agcaacacac actctctaca caaactaacc cagctctc                             8438


<210>  205
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 829

<400>  205
gttccaccgc ttccaggctt ggtacatgta c                                      31


<210>  206
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 830

<400>  206
gtacatgtac caagcctgga agcggtggaa c                                      31


<210>  207
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 831

<400>  207
ggagtgttgc gaacgacatg gagtgcgata tg                                     32


<210>  208
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YL 832

<400>  208
catatcgcac tccatgtcgt tcgcaacact cc                                     32


<210>  209
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  209
ttatccatcc agcatgattg ctcccacggg gccatcagc                              39


<210>  210
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  210
gctgatggcc ccgtgggagc aatcatgctg gatggataa                              39


<210>  211
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  211
tatccatcca gcatgatgac tcccacgggg ccatcag                                37


<210>  212
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  212
ctgatggccc cgtgggagtc atcatgctgg atggata                                37


<210>  213
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  213
tatccatcca gcatgatgag tcccacgggg ccatcag                                37


<210>  214
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  214
ctgatggccc cgtgggactc atcatgctgg atggata                                37


<210>  215
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  215
ttatccatcc agcatgattt ctcccacggg gccatcagc                              39


<210>  216
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  216
gctgatggcc ccgtgggaga aatcatgctg gatggataa                              39


<210>  217
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  217
ttatccatcc agcatgatgg ctcccacggg gccatcagc                              39


<210>  218
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  218
gctgatggcc ccgtgggagc catcatgctg gatggataa                              39


<210>  219
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  219
tatccatcca gcatgatcac tcccacgggg ccatcag                                37


<210>  220
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  220
ctgatggccc cgtgggagtg atcatgctgg atggata                                37


<210>  221
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  221
tatccatcca gcatgatatc tcccacgggg ccatcag                                37


<210>  222
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  222
ctgatggccc cgtgggagat atcatgctgg atggata                                37


<210>  223
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  223
tatccatcca gcatgataag tcccacgggg ccatcag                                37


<210>  224
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  224
ctgatggccc cgtgggactt atcatgctgg atggata                                37


<210>  225
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  225
tatccatcca gcatgatctg tcccacgggg ccatcag                                37


<210>  226
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  226
ctgatggccc cgtgggacag atcatgctgg atggata                                37


<210>  227
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  227
tatccatcca gcatgatatg tcccacgggg ccatcag                                37


<210>  228
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  228
ctgatggccc cgtgggacat atcatgctgg atggata                                37


<210>  229
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  229
tatccatcca gcatgataac tcccacgggg ccatcag                                37


<210>  230
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  230
ctgatggccc cgtgggagtt atcatgctgg atggata                                37


<210>  231
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  231
tatccatcca gcatgatccc tcccacgggg ccatcag                                37


<210>  232
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  232
ctgatggccc cgtgggaggg atcatgctgg atggata                                37


<210>  233
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  233
tatccatcca gcatgatcaa tcccacgggg ccatcag                                37


<210>  234
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  234
ctgatggccc cgtgggattg atcatgctgg atggata                                37


<210>  235
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  235
tatccatcca gcatgatcga tcccacgggg ccatcag                                37


<210>  236
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  236
ctgatggccc cgtgggatcg atcatgctgg atggata                                37


<210>  237
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  237
ttatccatcc agcatgattc ctcccacggg gccatcagc                              39


<210>  238
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  238
gctgatggcc ccgtgggagg aatcatgctg gatggataa                              39


<210>  239
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  239
tatccatcca gcatgatacc tcccacgggg ccatcag                                37


<210>  240
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  240
ctgatggccc cgtgggaggt atcatgctgg atggata                                37


<210>  241
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  241
tatccatcca gcatgatgtc tcccacgggg ccatcag                                37


<210>  242
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  242
ctgatggccc cgtgggagac atcatgctgg atggata                                37


<210>  243
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  243
tatccatcca gcatgattgg tcccacgggg ccatcag                                37


<210>  244
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  244
ctgatggccc cgtgggacca atcatgctgg atggata                                37


<210>  245
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  245
ttatccatcc agcatgatta ctcccacggg gccatcagc                              39


<210>  246
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  246
gctgatggcc ccgtgggagt aatcatgctg gatggataa                              39


<210>  247
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  247

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Gly Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  248
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  248

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ser Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  249
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  249
tccatccagc atgatgcggc tcacggggcc atcagcaag                              39


<210>  250
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  250
cttgctgatg gccccgtgag ccgcatcatg ctggatgga                              39


<210>  251
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  251
tccatccagc atgatgcgtg ccacggggcc atcagcaag                              39


<210>  252
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  252
cttgctgatg gccccgtggc acgcatcatg ctggatgga                              39


<210>  253
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  253
tccatccagc atgatgcgga ccacggggcc atcagcaag                              39


<210>  254
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  254
cttgctgatg gccccgtggt ccgcatcatg ctggatgga                              39


<210>  255
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  255
tccatccagc atgatgcgga gcacggggcc atcagcaag                              39


<210>  256
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  256
cttgctgatg gccccgtgct ccgcatcatg ctggatgga                              39


<210>  257
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  257
tccatccagc atgatgcgtt ccacggggcc atcagcaag                              39


<210>  258
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  258
cttgctgatg gccccgtgga acgcatcatg ctggatgga                              39


<210>  259
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  259
tccatccagc atgatgcggg tcacggggcc atcagcaag                              39


<210>  260
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  260
cttgctgatg gccccgtgac ccgcatcatg ctggatgga                              39


<210>  261
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  261
tccatccagc atgatgcgca ccacggggcc atcagcaag                              39


<210>  262
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  262
cttgctgatg gccccgtggt gcgcatcatg ctggatgga                              39


<210>  263
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  263
tccatccagc atgatgcgat ccacggggcc atcagcaag                              39


<210>  264
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  264
cttgctgatg gccccgtgga tcgcatcatg ctggatgga                              39


<210>  265
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  265
tccatccagc atgatgcgaa gcacggggcc atcagcaag                              39


<210>  266
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  266
cttgctgatg gccccgtgct tcgcatcatg ctggatgga                              39


<210>  267
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  267
tccatccagc atgatgcgct gcacggggcc atcagcaag                              39


<210>  268
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  268
cttgctgatg gccccgtgca gcgcatcatg ctggatgga                              39


<210>  269
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  269
tccatccagc atgatgcgat gcacggggcc atcagcaag                              39


<210>  270
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  270
cttgctgatg gccccgtgca tcgcatcatg ctggatgga                              39


<210>  271
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  271
tccatccagc atgatgcgaa ccacggggcc atcagcaag                              39


<210>  272
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  272
cttgctgatg gccccgtggt tcgcatcatg ctggatgga                              39


<210>  273
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  273
tccatccagc atgatgcgcc tcacggggcc atcagcaag                              39


<210>  274
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  274
cttgctgatg gccccgtgag gcgcatcatg ctggatgga                              39


<210>  275
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  275
tccatccagc atgatgcgca gcacggggcc atcagcaag                              39


<210>  276
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  276
cttgctgatg gccccgtgct gcgcatcatg ctggatgga                              39


<210>  277
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  277
tccatccagc atgatgcgcg acacggggcc atcagcaag                              39


<210>  278
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  278
cttgctgatg gccccgtgtc gcgcatcatg ctggatgga                              39


<210>  279
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  279
tccatccagc atgatgcgac ccacggggcc atcagcaag                              39


<210>  280
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  280
cttgctgatg gccccgtggg tcgcatcatg ctggatgga                              39


<210>  281
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  281
tccatccagc atgatgcggt ccacggggcc atcagcaag                              39


<210>  282
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  282
cttgctgatg gccccgtgga ccgcatcatg ctggatgga                              39


<210>  283
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  283
tccatccagc atgatgcgtg gcacggggcc atcagcaag                              39


<210>  284
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  284
cttgctgatg gccccgtgcc acgcatcatg ctggatgga                              39


<210>  285
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  285
tccatccagc atgatgcgta ccacggggcc atcagcaag                              39


<210>  286
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  286
cttgctgatg gccccgtggt acgcatcatg ctggatgga                              39


<210>  287
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  287

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ala His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  288
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  288

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  289
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367M4-157g

<400>  289
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga       60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct      120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc      180

ggagaactcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga      240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt      300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat      360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct      420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg gctcccacgg      480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg      540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca      600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc      660

cgccgcaaac accgcccgaa agtggttcca ccgcttccag gcttggtaca tgtaccttgt      720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca      780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta      840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg      900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc      960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga     1020

caagaactgc aaggttaagc gatctgagaa ggacgttgag gctgaccaaa ttgactggta     1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg     1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc     1200

cttcgtccag caggcggtcc gggagtgttg cgaacgacat ggagtgcgat atgttttcta     1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt     1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag     1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata     1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat     1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta     1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat     1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg     1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct     3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga     4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag     4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg     4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg     4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact     4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta     4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt     4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg     4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt     4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa     4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt     5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc     5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca     5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa     5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc     5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata     5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc     5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt     5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag     5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga     5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga     5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac     5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata     5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg     5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta     5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt     5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag     6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac     6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt     6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc     6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca     6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga     6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg     6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc     6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc     6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac     6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca     6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg     6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg     6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc     6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca     6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa     6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac     6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa     7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta     7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc     7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca     7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac     7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac     7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact     7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg     7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag     7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt     7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg     7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg     7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca     7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt     7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca     7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac     7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga     7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac     8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc     8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc     8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt     8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact     8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc     8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct     8400

agcaacacac actctctaca caaactaacc cagctctc                             8438


<210>  290
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367M4-158a

<400>  290
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga       60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct      120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc      180

ggagaactcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga      240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt      300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat      360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct      420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg cggctcacgg      480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg      540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca      600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc      660

cgccgcaaac accgcccgaa agtggttcca ccgcttccag gcttggtaca tgtaccttgt      720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca      780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta      840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg      900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc      960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga     1020

caagaactgc aaggttaagc gatctgagaa ggacgttgag gctgaccaaa ttgactggta     1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg     1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc     1200

cttcgtccag caggcggtcc gggagtgttg cgaacgacat ggagtgcgat atgttttcta     1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt     1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag     1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata     1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat     1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta     1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat     1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg     1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct     3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga     4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag     4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg     4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg     4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact     4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta     4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt     4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg     4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt     4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa     4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt     5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc     5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca     5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa     5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc     5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata     5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc     5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt     5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag     5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga     5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga     5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac     5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata     5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg     5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta     5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt     5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag     6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac     6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt     6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc     6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca     6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga     6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg     6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc     6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc     6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac     6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca     6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg     6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg     6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc     6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca     6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa     6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac     6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa     7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta     7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc     7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca     7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac     7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac     7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact     7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg     7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag     7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt     7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg     7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg     7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca     7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt     7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca     7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac     7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga     7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac     8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc     8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc     8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt     8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact     8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc     8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct     8400

agcaacacac actctctaca caaactaacc cagctctc                             8438


<210>  291
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367M4-158g

<400>  291
catggctctc agtcttacca cagaacagct gttagaacgc cctgatttgg ttgcgattga       60

tggcatcctc tacgaccttg aagggcttgc caaagttcat ccaggaggag atttgattct      120

cgcttctggt gcctctgatg cctcccctct cttttattca atgcatccat acgtcaaacc      180

ggagaactcc aaattgcttc aacagttcgt ccgagggaag catgaccgca cctcgaagga      240

cattgtctac acgtatgatt ctcccttcgc acaagacgtt aagcggacaa tgcgcgaggt      300

gatgaaaggg aggaactggt acgcaacccc tggcttctgg ctgcgcaccg ttgggatcat      360

cgccgtgacg gccttttgcg agtggcactg ggctaccacg gggatggtgc tgtggggcct      420

gttgactgga ttcatgcaca tgcagatcgg cttatccatc cagcatgatg cgggtcacgg      480

ggccatcagc aagaagcctt gggtcaacgc cctcttcgcc tacggcattg acgtcatcgg      540

atcgtcccgg tggatttggc tgcagtcgca catcatgcgg caccacacct acaccaacca      600

gcacggcctc gacctggatg cggagtcggc agagccgttc ctggtgttcc acaactaccc      660

cgccgcaaac accgcccgaa agtggttcca ccgcttccag gcttggtaca tgtaccttgt      720

gctgggggca tacggggtat cgctggtgta caacccgctc tacattttcc ggatgcagca      780

caatgacacc atcccagagt ctgtcacggc catgcgggaa aatggctttc tgcggcgcta      840

ccgcacactt gcattcgtga tgcgagcttt cttcatcttc cggaccgcat tcttgccctg      900

gtacctcact gggacctcat tgctgatcac cattcctctg gtgcccaccg caactggtgc      960

cttcttgacg ttcttcttca ttttgtccca caattttgat ggctccgaac ggatccccga     1020

caagaactgc aaggttaagc gatctgagaa ggacgttgag gctgaccaaa ttgactggta     1080

tcgggcgcag gtggagacgt cctccacata cggtggcccc atcgccatgt tcttcactgg     1140

cggtctcaat ttccagatcg agcaccacct ctttccccgg atgtcgtctt ggcactaccc     1200

cttcgtccag caggcggtcc gggagtgttg cgaacgacat ggagtgcgat atgttttcta     1260

ccctaccatc gtcggcaaca tcatctccac cctgaagtac atgcataagg tgggtgtcgt     1320

ccactgcgtg aaggacgcac aggattccta agcggccgca agtgtggatg gggaagtgag     1380

tgcccggttc tgtgtgcaca attggcaatc caagatggat ggattcaaca cagggatata     1440

gcgagctacg tggtggtgcg aggatatagc aacggatatt tatgtttgac acttgagaat     1500

gtacgataca agcactgtcc aagtacaata ctaaacatac tgtacatact catactcgta     1560

cccgggcaac ggtttcactt gagtgcagtg gctagtgctc ttactcgtac agtgtgcaat     1620

actgcgtatc atagtctttg atgtatatcg tattcattca tgttagttgc gtacgagccg     1680

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt     1740

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     1800

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg     1860

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa     1920

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc     1980

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     2520

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     2580

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     2640

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     2700

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     2760

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     2820

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     2880

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     2940

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     3000

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     3060

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     3120

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     3180

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     3240

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     3300

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     3360

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     3420

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     3480

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     3540

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     3600

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     3660

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     3720

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct     3780

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     3840

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     3900

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     3960

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     4020

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     4080

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     4140

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     4200

ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga     4260

agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc     4320

aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc     4380

cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt     4440

cgatggtgtc gataagcttg atatcgaatt catgtcacac aaaccgatct tcgcctcaag     4500

gaaacctaat tctacatccg agagactgcc gagatccagt ctacactgat taattttcgg     4560

gccaataatt taaaaaaatc gtgttatata atattatatg tattatatat atacatcatg     4620

atgatactga cagtcatgtc ccattgctaa atagacagac tccatctgcc gcctccaact     4680

gatgttctca atatttaagg ggtcatctcg cattgtttaa taataaacag actccatcta     4740

ccgcctccaa atgatgttct caaaatatat tgtatgaact tatttttatt acttagtatt     4800

attagacaac ttacttgctt tatgaaaaac acttcctatt taggaaacaa tttataatgg     4860

cagttcgttc atttaacaat ttatgtagaa taaatgttat aaatgcgtat gggaaatctt     4920

aaatatggat agcataaatg atatctgcat tgcctaattc gaaatcaaca gcaacgaaaa     4980

aaatcccttg tacaacataa atagtcatcg agaaatatca actatcaaag aacagctatt     5040

cacacgttac tattgagatt attattggac gagaatcaca cactcaactg tctttctctc     5100

ttctagaaat acaggtacaa gtatgtacta ttctcattgt tcatacttct agtcatttca     5160

tcccacatat tccttggatt tctctccaat gaatgacatt ctatcttgca aattcaacaa     5220

ttataataag atataccaaa gtagcggtat agtggcaatc aaaaagcttc tctggtgtgc     5280

ttctcgtatt tatttttatt ctaatgatcc attaaaggta tatatttatt tcttgttata     5340

taatcctttt gtttattaca tgggctggat acataaaggt attttgattt aattttttgc     5400

ttaaattcaa tcccccctcg ttcagtgtca actgtaatgg taggaaatta ccatactttt     5460

gaagaagcaa aaaaaatgaa agaaaaaaaa aatcgtattt ccaggttaga cgttccgcag     5520

aatctagaat gcggtatgcg gtacattgtt cttcgaacgt aaaagttgcg ctccctgaga     5580

tattgtacat ttttgctttt acaagtacaa gtacatcgta caactatgta ctactgttga     5640

tgcatccaca acagtttgtt ttgttttttt ttgttttttt tttttctaat gattcattac     5700

cgctatgtat acctacttgt acttgtagta agccgggtta ttggcgttca attaatcata     5760

gacttatgaa tctgcacggt gtgcgctgcg agttactttt agcttatgca tgctacttgg     5820

gtgtaatatt gggatctgtt cggaaatcaa cggatgctca atcgatttcg acagtaatta     5880

attaagtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt     5940

attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag     6000

atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac     6060

catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt     6120

acatatccat agtctaacct ctaacagtta atcttctggt aagcctccca gccagccttc     6180

tggtatcgct tggcctcctc aataggatct cggttctggc cgtacagacc tcggccgaca     6240

attatgatat ccgttccggt agacatgaca tcctcaacag ttcggtactg ctgtccgaga     6300

gcgtctccct tgtcgtcaag acccaccccg ggggtcagaa taagccagtc ctcagagtcg     6360

cccttaggtc ggttctgggc aatgaagcca accacaaact cggggtcgga tcgggcaagc     6420

tcaatggtct gcttggagta ctcgccagtg gccagagagc ccttgcaaga cagctcggcc     6480

agcatgagca gacctctggc cagcttctcg ttgggagagg ggactaggaa ctccttgtac     6540

tgggagttct cgtagtcaga gacgtcctcc ttcttctgtt cagagacagt ttcctcggca     6600

ccagctcgca ggccagcaat gattccggtt ccgggtacac cgtgggcgtt ggtgatatcg     6660

gaccactcgg cgattcggtg acaccggtac tggtgcttga cagtgttgcc aatatctgcg     6720

aactttctgt cctcgaacag gaagaaaccg tgcttaagag caagttcctt gagggggagc     6780

acagtgccgg cgtaggtgaa gtcgtcaatg atgtcgatat gggttttgat catgcacaca     6840

taaggtccga ccttatcggc aagctcaatg agctccttgg tggtggtaac atccagagaa     6900

gcacacaggt tggttttctt ggctgccacg agcttgagca ctcgagcggc aaaggcggac     6960

ttgtggacgt tagctcgagc ttcgtaggag ggcattttgg tggtgaagag gagactgaaa     7020

taaatttagt ctgcagaact ttttatcgga accttatctg gggcagtgaa gtatatgtta     7080

tggtaatagt tacgagttag ttgaacttat agatagactg gactatacgg ctatcggtcc     7140

aaattagaaa gaacgtcaat ggctctctgg gcgtcgcctt tgccgacaaa aatgtgatca     7200

tgatgaaagc cagcaatgac gttgcagctg atattgttgt cggccaaccg cgccgaaaac     7260

gcagctgtca gacccacagc ctccaacgaa gaatgtatcg tcaaagtgat ccaagcacac     7320

tcatagttgg agtcgtactc caaaggcggc aatgacgagt cagacagata ctcgtcgact     7380

caggcgacga cggaattcct gcagcccatc tgcagaattc aggagagacc gggttggcgg     7440

cgtatttgtg tcccaaaaaa cagccccaat tgccccggag aagacggcca ggccgcctag     7500

atgacaaatt caacaactca cagctgactt tctgccattg ccactagggg ggggcctttt     7560

tatatggcca agccaagctc tccacgtcgg ttgggctgca cccaacaata aatgggtagg     7620

gttgcaccaa caaagggatg ggatgggggg tagaagatac gaggataacg gggctcaatg     7680

gcacaaataa gaacgaatac tgccattaag actcgtgatc cagcgactga caccattgca     7740

tcatctaagg gcctcaaaac tacctcggaa ctgctgcgct gatctggaca ccacagaggt     7800

tccgagcact ttaggttgca ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca     7860

gcgtgtacag tttgtcttaa caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac     7920

ttgttatagc ctttagagct gcgaaagcgc gtatggattt ggctcatcag gccagattga     7980

gggtctgtgg acacatgtca tgttagtgta cttcaatcgc cccctggata tagccccgac     8040

aataggccgt ggcctcattt ttttgccttc cgcacatttc cattgctcgg tacccacacc     8100

ttgcttctcc tgcacttgcc aaccttaata ctggtttaca ttgaccaaca tcttacaagc     8160

ggggggcttg tctagggtat atataaacag tggctctccc aatcggttgc cagtctcttt     8220

tttcctttct ttccccacag attcgaaatc taaactacac atcacacaat gcctgttact     8280

gacgtcctta agcgaaagtc cggtgtcatc gtcggcgacg atgtccgagc cgtgagtatc     8340

cacgacaaga tcagtgtcga gacgacgcgt tttgtgtaat gacacaatcc gaaagtcgct     8400

agcaacacac actctctaca caaactaacc cagctctc                             8438


<210>  292
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  292
ggcttgccaa agttcatggc ggaggagatt tgattctc                               38


<210>  293
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  293
gagaatcaaa tctcctccgc catgaacttt ggcaagcc                               38


<210>  294
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  294
ggcttgccaa agttcatcac ggaggagatt tgattctc                               38


<210>  295
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  295
gagaatcaaa tctcctccgt gatgaacttt ggcaagcc                               38


<210>  296
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  296
ccaaagttca tccaggatcc gatttgattc tcgcttct                               38


<210>  297
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  297
agaagcgaga atcaaatcgg atcctggatg aactttgg                               38


<210>  298
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  298
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ggt gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aac tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat ggc tcc cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Gly Ser His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag cga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  299
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  299

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Gly Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  300
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  300
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ggt gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aac tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg gct cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ala His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag cga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  301
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  301

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ala His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  302
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  302
atg gct ctc agt ctt acc aca gaa cag ctg tta gaa cgc cct gat ttg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcg att gat ggc atc ctc tac gac ctt gaa ggg ctt gcc aaa gtt         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ggt gga gga gat ttg att ctc gct tct ggt gcc tct gat gcc tcc        144
His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctc ttt tat tca atg cat cca tac gtc aaa ccg gag aac tcc aaa        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ttg ctt caa cag ttc gtc cga ggg aag cat gac cgc acc tcg aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acg tat gat tct ccc ttc gca caa gac gtt aag cgg aca        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cgc gag gtg atg aaa ggg agg aac tgg tac gca acc cct ggc ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cgc acc gtt ggg atc atc gcc gtg acg gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct acc acg ggg atg gtg ctg tgg ggc ctg ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc tta tcc atc cag cat gat gcg ggt cac ggg        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly           
145                 150                 155                 160           

gcc atc agc aag aag cct tgg gtc aac gcc ctc ttc gcc tac ggc att        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc atc gga tcg tcc cgg tgg att tgg ctg cag tcg cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cgg cac cac acc tac acc aac cag cac ggc ctc gac ctg gat gcg gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcg gca gag ccg ttc ctg gtg ttc cac aac tac ccc gcc gca aac acc        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gcc cga aag tgg ttc cac cgc ttc cag gct tgg tac atg tac ctt gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctg ggg gca tac ggg gta tcg ctg gtg tac aac ccg ctc tac att ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cgg atg cag cac aat gac acc atc cca gag tct gtc acg gcc atg cgg        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gaa aat ggc ttt ctg cgg cgc tac cgc aca ctt gca ttc gtg atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttc cgg acc gca ttc ttg ccc tgg tac ctc act ggg        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tca ttg ctg atc acc att cct ctg gtg ccc acc gca act ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ttg acg ttc ttc ttc att ttg tcc cac aat ttt gat ggc tcc gaa       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cgg atc ccc gac aag aac tgc aag gtt aag cga tct gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val           
            340                 345                 350                   

gag gct gac caa att gac tgg tat cgg gcg cag gtg gag acg tcc tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

aca tac ggt ggc ccc atc gcc atg ttc ttc act ggc ggt ctc aat ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cac cac ctc ttt ccc cgg atg tcg tct tgg cac tac ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtc cag cag gcg gtc cgg gag tgt tgc gaa cga cat gga gtg cga       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tat gtt ttc tac cct acc atc gtc ggc aac atc atc tcc acc ctg aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cat aag gtg ggt gtc gtc cac tgc gtg aag gac gca cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  303
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  303

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Gly Gly Gly Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Arg Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  304
<211>  4070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pEgD5M

<400>  304
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccat ggccctgtct ctcactaccg      420

aacagctcct ggagcgacct gatctcgtcg ctatcgatgg tatcctgtac gacctcgagg      480

gcctggccaa ggtgcatggt ggtggagacc tcattctggc ctctggagcc tccgacgcct      540

ctcccctctt ctactctatg catccctacg tcaagcccga gaactccaag ctcctgcagc      600

aattcgtccg agggaagcat gaccgcacct cgaaggacat tgtctacacg tatgattctc      660

ccttcgcaca agacgttaag cggacaatgc gcgaggtgat gaaagggagg aactggtacg      720

caacccctgg cttctggctg cgcaccgttg ggatcatcgc cgtgacggcc ttttgcgagt      780

ggcactgggc taccacgggg atggtgctgt ggggcctgtt gactggattc atgcacatgc      840

agatcggctt atccatccag catgatgcgg gtcacggggc catcagcaag aagccttggg      900

tcaacgccct cttcgcctac ggcattgacg tcatcggatc gtcccggtgg atttggctgc      960

agtcgcacat catgcggcac cacacctaca ccaaccagca cggcctcgac ctggatgcgg     1020

agtcggcaga gccgttcctg gtgttccaca actaccccgc cgcaaacacc gcccgaaagt     1080

ggttccaccg cttccaggct tggtacatgt accttgtgct gggggcatac ggggtatcgc     1140

tggtgtacaa cccgctctac attttccgga tgcagcacaa tgacaccatc ccagagtctg     1200

tcacggccat gcgggaaaat ggctttctgc ggcgctaccg cacacttgca ttcgtgatgc     1260

gagctttctt catcttccgg accgcattct tgccctggta cctcactggg acctcattgc     1320

tgatcaccat tcctctggtg cccaccgcaa ctggtgcctt cttgacgttc ttcttcattt     1380

tgtcccacaa ttttgatggc tccgaacgga tccccgacaa gaactgcaag gttaagcgat     1440

ctgagaagga cgttgaggct gaccaaattg actggtatcg ggcgcaggtg gagacgtcct     1500

ccacatacgg tggccccatc gccatgttct tcactggcgg tctcaatttc cagatcgagc     1560

accacctctt tccccggatg tcgtcttggc actacccctt cgtccagcag gcggtccggg     1620

agtgttgcga acgacatgga gtgcgatatg ttttctaccc taccatcgtc ggcaacatca     1680

tctccaccct gaagtacatg cataaggtgg gtgtcgtcca ctgcgtgaag gacgcacagg     1740

attcctaagc ggccgcaatt cgagctcggt acctcgcgaa tgcatctaga tatcggatcc     1800

cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt     1860

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa     1920

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact     1980

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc     2040

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg     2100

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     2160

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     2220

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     2280

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     2340

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     2400

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     2460

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     2520

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     2580

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     2640

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt     2700

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     2760

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     2820

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     2880

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     2940

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     3000

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     3060

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     3120

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     3180

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     3240

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     3300

tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat     3360

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     3420

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     3480

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     3540

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     3600

accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt     3660

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     3720

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     3780

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     3840

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     3900

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     3960

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat     4020

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc                4070


<210>  305
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367-5M

<400>  305
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgactcag gcgacgacgg aattcctgca gcccatctgc     6060

agaattcagg agagaccggg ttggcggcgt atttgtgtcc caaaaaacag ccccaattgc     6120

cccggagaag acggccaggc cgcctagatg acaaattcaa caactcacag ctgactttct     6180

gccattgcca ctaggggggg gcctttttat atggccaagc caagctctcc acgtcggttg     6240

ggctgcaccc aacaataaat gggtagggtt gcaccaacaa agggatggga tggggggtag     6300

aagatacgag gataacgggg ctcaatggca caaataagaa cgaatactgc cattaagact     6360

cgtgatccag cgactgacac cattgcatca tctaagggcc tcaaaactac ctcggaactg     6420

ctgcgctgat ctggacacca cagaggttcc gagcacttta ggttgcacca aatgtcccac     6480

caggtgcagg cagaaaacgc tggaacagcg tgtacagttt gtcttaacaa aaagtgaggg     6540

cgctgaggtc gagcagggtg gtgtgacttg ttatagcctt tagagctgcg aaagcgcgta     6600

tggatttggc tcatcaggcc agattgaggg tctgtggaca catgtcatgt tagtgtactt     6660

caatcgcccc ctggatatag ccccgacaat aggccgtggc ctcatttttt tgccttccgc     6720

acatttccat tgctcggtac ccacaccttg cttctcctgc acttgccaac cttaatactg     6780

gtttacattg accaacatct tacaagcggg gggcttgtct agggtatata taaacagtgg     6840

ctctcccaat cggttgccag tctctttttt cctttctttc cccacagatt cgaaatctaa     6900

actacacatc acacaatgcc tgttactgac gtccttaagc gaaagtccgg tgtcatcgtc     6960

ggcgacgatg tccgagccgt gagtatccac gacaagatca gtgtcgagac gacgcgtttt     7020

gtgtaatgac acaatccgaa agtcgctagc aacacacact ctctacacaa actaacccag     7080

ctctccatgg ccctgtctct cactaccgaa cagctcctgg agcgacctga tctcgtcgct     7140

atcgatggta tcctgtacga cctcgagggc ctggccaagg tgcatggtgg tggagacctc     7200

attctggcct ctggagcctc cgacgcctct cccctcttct actctatgca tccctacgtc     7260

aagcccgaga actccaagct cctgcagcaa ttcgtccgag ggaagcatga ccgcacctcg     7320

aaggacattg tctacacgta tgattctccc ttcgcacaag acgttaagcg gacaatgcgc     7380

gaggtgatga aagggaggaa ctggtacgca acccctggct tctggctgcg caccgttggg     7440

atcatcgccg tgacggcctt ttgcgagtgg cactgggcta ccacggggat ggtgctgtgg     7500

ggcctgttga ctggattcat gcacatgcag atcggcttat ccatccagca tgatgcgggt     7560

cacggggcca tcagcaagaa gccttgggtc aacgccctct tcgcctacgg cattgacgtc     7620

atcggatcgt cccggtggat ttggctgcag tcgcacatca tgcggcacca cacctacacc     7680

aaccagcacg gcctcgacct ggatgcggag tcggcagagc cgttcctggt gttccacaac     7740

taccccgccg caaacaccgc ccgaaagtgg ttccaccgct tccaggcttg gtacatgtac     7800

cttgtgctgg gggcatacgg ggtatcgctg gtgtacaacc cgctctacat tttccggatg     7860

cagcacaatg acaccatccc agagtctgtc acggccatgc gggaaaatgg ctttctgcgg     7920

cgctaccgca cacttgcatt cgtgatgcga gctttcttca tcttccggac cgcattcttg     7980

ccctggtacc tcactgggac ctcattgctg atcaccattc ctctggtgcc caccgcaact     8040

ggtgccttct tgacgttctt cttcattttg tcccacaatt ttgatggctc cgaacggatc     8100

cccgacaaga actgcaaggt taagcgatct gagaaggacg ttgaggctga ccaaattgac     8160

tggtatcggg cgcaggtgga gacgtcctcc acatacggtg gccccatcgc catgttcttc     8220

actggcggtc tcaatttcca gatcgagcac cacctctttc cccggatgtc gtcttggcac     8280

taccccttcg tccagcaggc ggtccgggag tgttgcgaac gacatggagt gcgatatgtt     8340

ttctacccta ccatcgtcgg caacatcatc tccaccctga agtacatgca taaggtgggt     8400

gtcgtccact gcgtgaagga cgcacaggat tcctaagc                             8438


<210>  306
<211>  4070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pEgD5M1

<400>  306
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccat ggccctgtct ctcactaccg      420

aacagctcct ggagcgacct gatctcgtcg ctatcgatgg tatcctgtac gacctcgagg      480

gcctggccaa ggtgcatggt ggtggagacc tcattctggc ctctggagcc tccgacgcct      540

ctcccctctt ctactctatg catccctacg tcaagcccga gaactccaag ctcctgcagc      600

aattcgtccg agggaagcat gaccgcacct cgaaggacat tgtctacacg tatgattctc      660

ccttcgcaca agacgttaag cggacaatgc gcgaggtgat gaaagggagg aactggtacg      720

caacccctgg cttctggctg cgcaccgttg ggatcatcgc cgtgacggcc ttttgcgagt      780

ggcactgggc taccacgggg atggtgctgt ggggcctgtt gactggattc atgcacatgc      840

agatcggctt atccatccag catgatgcgg gtcacggggc catcagcaag aagccttggg      900

tcaacgccct cttcgcctac ggcattgacg tcatcggatc gtcccggtgg atttggctgc      960

agtcgcacat catgcggcac cacacctaca ccaaccagca cggcctcgac ctggatgcgg     1020

agtcggcaga gccgttcctg gtgttccaca actaccccgc cgcaaacacc gcccgaaagt     1080

ggttccaccg cttccaggct tggtacatgt accttgtgct gggggcatac ggggtatcgc     1140

tggtgtacaa cccgctctac attttccgga tgcagcacaa tgacaccatc ccagagtctg     1200

tcacggccat gcgggaaaat ggctttctgc ggcgctaccg cacacttgca ttcgtgatgc     1260

gagctttctt catcttccgg accgcattct tgccctggta cctcactggg acctcattgc     1320

tgatcaccat tcctctggtg cccaccgcaa ctggtgcctt cttgacgttc ttcttcattt     1380

tgtcccacaa ttttgatggc tccgaacgga tccccgacaa gaactgcaag gttaagagct     1440

ctgagaagga cgttgaggct gaccaaattg actggtatcg ggcgcaggtg gagacgtcct     1500

ccacatacgg tggccccatc gccatgttct tcactggcgg tctcaatttc cagatcgagc     1560

accacctctt tccccggatg tcgtcttggc actacccctt cgtccagcag gcggtccggg     1620

agtgttgcga acgacatgga gtgcgatatg ttttctaccc taccatcgtc ggcaacatca     1680

tctccaccct gaagtacatg cataaggtgg gtgtcgtcca ctgcgtgaag gacgcacagg     1740

attcctaagc ggccgcaatt cgagctcggt acctcgcgaa tgcatctaga tatcggatcc     1800

cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt     1860

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa     1920

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact     1980

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc     2040

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg     2100

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     2160

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     2220

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     2280

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     2340

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     2400

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     2460

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     2520

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     2580

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     2640

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt     2700

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     2760

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     2820

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     2880

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     2940

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     3000

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     3060

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     3120

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     3180

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     3240

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     3300

tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat     3360

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     3420

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     3480

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     3540

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     3600

accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt     3660

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     3720

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     3780

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     3840

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     3900

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     3960

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat     4020

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc                4070


<210>  307
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW367-5M1

<400>  307
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgactcag gcgacgacgg aattcctgca gcccatctgc     6060

agaattcagg agagaccggg ttggcggcgt atttgtgtcc caaaaaacag ccccaattgc     6120

cccggagaag acggccaggc cgcctagatg acaaattcaa caactcacag ctgactttct     6180

gccattgcca ctaggggggg gcctttttat atggccaagc caagctctcc acgtcggttg     6240

ggctgcaccc aacaataaat gggtagggtt gcaccaacaa agggatggga tggggggtag     6300

aagatacgag gataacgggg ctcaatggca caaataagaa cgaatactgc cattaagact     6360

cgtgatccag cgactgacac cattgcatca tctaagggcc tcaaaactac ctcggaactg     6420

ctgcgctgat ctggacacca cagaggttcc gagcacttta ggttgcacca aatgtcccac     6480

caggtgcagg cagaaaacgc tggaacagcg tgtacagttt gtcttaacaa aaagtgaggg     6540

cgctgaggtc gagcagggtg gtgtgacttg ttatagcctt tagagctgcg aaagcgcgta     6600

tggatttggc tcatcaggcc agattgaggg tctgtggaca catgtcatgt tagtgtactt     6660

caatcgcccc ctggatatag ccccgacaat aggccgtggc ctcatttttt tgccttccgc     6720

acatttccat tgctcggtac ccacaccttg cttctcctgc acttgccaac cttaatactg     6780

gtttacattg accaacatct tacaagcggg gggcttgtct agggtatata taaacagtgg     6840

ctctcccaat cggttgccag tctctttttt cctttctttc cccacagatt cgaaatctaa     6900

actacacatc acacaatgcc tgttactgac gtccttaagc gaaagtccgg tgtcatcgtc     6960

ggcgacgatg tccgagccgt gagtatccac gacaagatca gtgtcgagac gacgcgtttt     7020

gtgtaatgac acaatccgaa agtcgctagc aacacacact ctctacacaa actaacccag     7080

ctctccatgg ccctgtctct cactaccgaa cagctcctgg agcgacctga tctcgtcgct     7140

atcgatggta tcctgtacga cctcgagggc ctggccaagg tgcatggtgg tggagacctc     7200

attctggcct ctggagcctc cgacgcctct cccctcttct actctatgca tccctacgtc     7260

aagcccgaga actccaagct cctgcagcaa ttcgtccgag ggaagcatga ccgcacctcg     7320

aaggacattg tctacacgta tgattctccc ttcgcacaag acgttaagcg gacaatgcgc     7380

gaggtgatga aagggaggaa ctggtacgca acccctggct tctggctgcg caccgttggg     7440

atcatcgccg tgacggcctt ttgcgagtgg cactgggcta ccacggggat ggtgctgtgg     7500

ggcctgttga ctggattcat gcacatgcag atcggcttat ccatccagca tgatgcgggt     7560

cacggggcca tcagcaagaa gccttgggtc aacgccctct tcgcctacgg cattgacgtc     7620

atcggatcgt cccggtggat ttggctgcag tcgcacatca tgcggcacca cacctacacc     7680

aaccagcacg gcctcgacct ggatgcggag tcggcagagc cgttcctggt gttccacaac     7740

taccccgccg caaacaccgc ccgaaagtgg ttccaccgct tccaggcttg gtacatgtac     7800

cttgtgctgg gggcatacgg ggtatcgctg gtgtacaacc cgctctacat tttccggatg     7860

cagcacaatg acaccatccc agagtctgtc acggccatgc gggaaaatgg ctttctgcgg     7920

cgctaccgca cacttgcatt cgtgatgcga gctttcttca tcttccggac cgcattcttg     7980

ccctggtacc tcactgggac ctcattgctg atcaccattc ctctggtgcc caccgcaact     8040

ggtgccttct tgacgttctt cttcattttg tcccacaatt ttgatggctc cgaacggatc     8100

cccgacaaga actgcaaggt taagagctct gagaaggacg ttgaggctga ccaaattgac     8160

tggtatcggg cgcaggtgga gacgtcctcc acatacggtg gccccatcgc catgttcttc     8220

actggcggtc tcaatttcca gatcgagcac cacctctttc cccggatgtc gtcttggcac     8280

taccccttcg tccagcaggc ggtccgggag tgttgcgaac gacatggagt gcgatatgtt     8340

ttctacccta ccatcgtcgg caacatcatc tccaccctga agtacatgca taaggtgggt     8400

gtcgtccact gcgtgaagga cgcacaggat tcctaagc                             8438


<210>  308
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  308

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  309
<211>  8438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pDMW369S

<400>  309
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgactcag gcgacgacgg aattcctgca gcccatctgc     6060

agaattcagg agagaccggg ttggcggcgt atttgtgtcc caaaaaacag ccccaattgc     6120

cccggagaag acggccaggc cgcctagatg acaaattcaa caactcacag ctgactttct     6180

gccattgcca ctaggggggg gcctttttat atggccaagc caagctctcc acgtcggttg     6240

ggctgcaccc aacaataaat gggtagggtt gcaccaacaa agggatggga tggggggtag     6300

aagatacgag gataacgggg ctcaatggca caaataagaa cgaatactgc cattaagact     6360

cgtgatccag cgactgacac cattgcatca tctaagggcc tcaaaactac ctcggaactg     6420

ctgcgctgat ctggacacca cagaggttcc gagcacttta ggttgcacca aatgtcccac     6480

caggtgcagg cagaaaacgc tggaacagcg tgtacagttt gtcttaacaa aaagtgaggg     6540

cgctgaggtc gagcagggtg gtgtgacttg ttatagcctt tagagctgcg aaagcgcgta     6600

tggatttggc tcatcaggcc agattgaggg tctgtggaca catgtcatgt tagtgtactt     6660

caatcgcccc ctggatatag ccccgacaat aggccgtggc ctcatttttt tgccttccgc     6720

acatttccat tgctcggtac ccacaccttg cttctcctgc acttgccaac cttaatactg     6780

gtttacattg accaacatct tacaagcggg gggcttgtct agggtatata taaacagtgg     6840

ctctcccaat cggttgccag tctctttttt cctttctttc cccacagatt cgaaatctaa     6900

actacacatc acacaatgcc tgttactgac gtccttaagc gaaagtccgg tgtcatcgtc     6960

ggcgacgatg tccgagccgt gagtatccac gacaagatca gtgtcgagac gacgcgtttt     7020

gtgtaatgac acaatccgaa agtcgctagc aacacacact ctctacacaa actaacccag     7080

ctctccatgg ctctctccct tactaccgag cagctgctcg agcgacccga cctggttgcc     7140

atcgacggca ttctctacga tctggaaggt cttgccaagg tccatcccgg atccgacttg     7200

atcctcgctt ctggtgcctc cgatgcttct cctctgttct actccatgca cccttacgtc     7260

aagcccgaga actcgaagct gcttcaacag ttcgtgcgag gcaagcacga ccgaacctcc     7320

aaggacattg tctacaccta cgactctccc tttgcacagg acgtcaagcg aactatgcga     7380

gaggtcatga aaggtcggaa ctggtatgcc acacctggat tctggctgcg aaccgttggc     7440

atcattgctg tcaccgcctt ttgcgagtgg cactgggcta ctaccggaat ggtgctgtgg     7500

ggtctcttga ctggattcat gcacatgcag atcggcctgt ccattcagca cgatgcctct     7560

catggtgcca tcagcaaaaa gccctgggtc aacgctctct ttgcctacgg catcgacgtc     7620

attggatcgt ccagatggat ctggctgcag tctcacatca tgcgacatca cacctacacc     7680

aatcagcatg gtctcgacct ggatgccgag tccgcagaac cattccttgt gttccacaac     7740

taccctgctg ccaacactgc tcgaaagtgg tttcaccgat tccaggcctg gtacatgtac     7800

ctcgtgcttg gagcctacgg cgtttcgctg gtgtacaacc ctctctacat cttccgaatg     7860

cagcacaacg acaccattcc cgagtctgtc acagccatgc gagagaacgg ctttctgcga     7920

cggtaccgaa cccttgcatt cgttatgcga gctttcttca tctttcgaac cgccttcttg     7980

ccctggtatc tcactggaac ctccctgctc atcaccattc ctctggtgcc cactgctacc     8040

ggtgccttcc tcaccttctt tttcatcttg tctcacaact tcgatggctc ggagcgaatc     8100

cccgacaaga actgcaaggt caagagctcc gagaaggacg ttgaagccga tcagatcgac     8160

tggtacagag ctcaggtgga gacctcttcc acctacggtg gacccattgc catgttcttt     8220

actggcggtc tcaacttcca gatcgagcat cacctctttc ctcgaatgtc gtcttggcac     8280

tatcccttcg tgcagcaagc tgtccgagag tgttgcgaac gacacggagt tcggtacgtc     8340

ttctacccta ccattgtggg caacatcatt tccaccctca agtacatgca caaagtcggt     8400

gtggttcact gtgtcaagga cgctcaggat tcctaagc                             8438


<210>  310
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  310
cctgtccatt cagcacgatt tctctcatgg tgccatcag                              39


<210>  311
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  311
ctgatggcac catgagagaa atcgtgctga atggacagg                              39


<210>  312
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  312
cctgtccatt cagcacgata tgtctcatgg tgccatcag                              39


<210>  313
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  313
ctgatggcac catgagacat atcgtgctga atggacagg                              39


<210>  314
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  314
gtccattcag cacgatggtt ctcatggtgc catcag                                 36


<210>  315
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  315
ctgatggcac catgagaacc atcgtgctga atggac                                 36


<210>  316
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  316
gtccattcag cacgattcct ctcatggtgc c                                      31


<210>  317
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  317
ggcaccatga gaggaatcgt gctgaatgga c                                      31


<210>  318
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  318
cattcagcac gatgccgctc atggtgccat cag                                    33


<210>  319
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  319
ctgatggcac catgagcggc atcgtgctga atg                                    33


<210>  320
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  320
ctgtccattc agcacgatgc caaccatggt gccatcagca aaaag                       45


<210>  321
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  321
ctttttgctg atggcaccat ggttggcatc gtgctgaatg gacag                       45


<210>  322
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  322
cattcagcac gatgccaccc atggtgccat cagca                                  35


<210>  323
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  323
tgctgatggc accatgggtg gcatcgtgct gaatg                                  35


<210>  324
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  324
cattcagcac gatgccggtc atggtgccat cagc                                   34


<210>  325
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  325
gctgatggca ccatgaccgg catcgtgctg aatg                                   34


<210>  326
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  326
ctgtccattc agcacgaggc ctctcatggt g                                      31


<210>  327
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  327
caccatgaga ggcctcgtgc tgaatggaca g                                      31


<210>  328
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  328
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga tcc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gag gcc tct cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Glu Ala Ser His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  329
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  329

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Glu Ala Ser His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  330
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  330
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga tcc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc gct cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ala His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  331
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  331

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Ala His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  332
<211>  1350
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  332
atg gct ctc tcc ctt act acc gag cag ctg ctc gag cga ccc gac ctg         48
Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu           
1               5                   10                  15                

gtt gcc atc gac ggc att ctc tac gat ctg gaa ggt ctt gcc aag gtc         96
Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val           
            20                  25                  30                    

cat ccc gga tcc gac ttg atc ctc gct tct ggt gcc tcc gat gct tct        144
His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser           
        35                  40                  45                        

cct ctg ttc tac tcc atg cac cct tac gtc aag ccc gag aac tcg aag        192
Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys           
    50                  55                  60                            

ctg ctt caa cag ttc gtg cga ggc aag cac gac cga acc tcc aag gac        240
Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp           
65                  70                  75                  80            

att gtc tac acc tac gac tct ccc ttt gca cag gac gtc aag cga act        288
Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr           
                85                  90                  95                

atg cga gag gtc atg aaa ggt cgg aac tgg tat gcc aca cct gga ttc        336
Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe           
            100                 105                 110                   

tgg ctg cga acc gtt ggc atc att gct gtc acc gcc ttt tgc gag tgg        384
Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp           
        115                 120                 125                       

cac tgg gct act acc gga atg gtg ctg tgg ggt ctc ttg act gga ttc        432
His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe           
    130                 135                 140                           

atg cac atg cag atc ggc ctg tcc att cag cac gat gcc ggt cat ggt        480
Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly           
145                 150                 155                 160           

gcc atc agc aaa aag ccc tgg gtc aac gct ctc ttt gcc tac ggc atc        528
Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile           
                165                 170                 175               

gac gtc att gga tcg tcc aga tgg atc tgg ctg cag tct cac atc atg        576
Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met           
            180                 185                 190                   

cga cat cac acc tac acc aat cag cat ggt ctc gac ctg gat gcc gag        624
Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu           
        195                 200                 205                       

tcc gca gaa cca ttc ctt gtg ttc cac aac tac cct gct gcc aac act        672
Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr           
    210                 215                 220                           

gct cga aag tgg ttt cac cga ttc cag gcc tgg tac atg tac ctc gtg        720
Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val           
225                 230                 235                 240           

ctt gga gcc tac ggc gtt tcg ctg gtg tac aac cct ctc tac atc ttc        768
Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe           
                245                 250                 255               

cga atg cag cac aac gac acc att ccc gag tct gtc aca gcc atg cga        816
Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg           
            260                 265                 270                   

gag aac ggc ttt ctg cga cgg tac cga acc ctt gca ttc gtt atg cga        864
Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg           
        275                 280                 285                       

gct ttc ttc atc ttt cga acc gcc ttc ttg ccc tgg tat ctc act gga        912
Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly           
    290                 295                 300                           

acc tcc ctg ctc atc acc att cct ctg gtg ccc act gct acc ggt gcc        960
Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala           
305                 310                 315                 320           

ttc ctc acc ttc ttt ttc atc ttg tct cac aac ttc gat ggc tcg gag       1008
Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu           
                325                 330                 335               

cga atc ccc gac aag aac tgc aag gtc aag agc tcc gag aag gac gtt       1056
Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val           
            340                 345                 350                   

gaa gcc gat cag atc gac tgg tac aga gct cag gtg gag acc tct tcc       1104
Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser           
        355                 360                 365                       

acc tac ggt gga ccc att gcc atg ttc ttt act ggc ggt ctc aac ttc       1152
Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe           
    370                 375                 380                           

cag atc gag cat cac ctc ttt cct cga atg tcg tct tgg cac tat ccc       1200
Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro           
385                 390                 395                 400           

ttc gtg cag caa gct gtc cga gag tgt tgc gaa cga cac gga gtt cgg       1248
Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg           
                405                 410                 415               

tac gtc ttc tac cct acc att gtg ggc aac atc att tcc acc ctc aag       1296
Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys           
            420                 425                 430                   

tac atg cac aaa gtc ggt gtg gtt cac tgt gtc aag gac gct cag gat       1344
Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp           
        435                 440                 445                       

tcc taa                                                               1350
Ser                                                                       
                                                                          


<210>  333
<211>  449
<212>  PRT
<213>  Euglena gracilis

<400>  333

Met Ala Leu Ser Leu Thr Thr Glu Gln Leu Leu Glu Arg Pro Asp Leu 
1               5                   10                  15      


Val Ala Ile Asp Gly Ile Leu Tyr Asp Leu Glu Gly Leu Ala Lys Val 
            20                  25                  30          


His Pro Gly Ser Asp Leu Ile Leu Ala Ser Gly Ala Ser Asp Ala Ser 
        35                  40                  45              


Pro Leu Phe Tyr Ser Met His Pro Tyr Val Lys Pro Glu Asn Ser Lys 
    50                  55                  60                  


Leu Leu Gln Gln Phe Val Arg Gly Lys His Asp Arg Thr Ser Lys Asp 
65                  70                  75                  80  


Ile Val Tyr Thr Tyr Asp Ser Pro Phe Ala Gln Asp Val Lys Arg Thr 
                85                  90                  95      


Met Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly Phe 
            100                 105                 110         


Trp Leu Arg Thr Val Gly Ile Ile Ala Val Thr Ala Phe Cys Glu Trp 
        115                 120                 125             


His Trp Ala Thr Thr Gly Met Val Leu Trp Gly Leu Leu Thr Gly Phe 
    130                 135                 140                 


Met His Met Gln Ile Gly Leu Ser Ile Gln His Asp Ala Gly His Gly 
145                 150                 155                 160 


Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Leu Phe Ala Tyr Gly Ile 
                165                 170                 175     


Asp Val Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile Met 
            180                 185                 190         


Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala Glu 
        195                 200                 205             


Ser Ala Glu Pro Phe Leu Val Phe His Asn Tyr Pro Ala Ala Asn Thr 
    210                 215                 220                 


Ala Arg Lys Trp Phe His Arg Phe Gln Ala Trp Tyr Met Tyr Leu Val 
225                 230                 235                 240 


Leu Gly Ala Tyr Gly Val Ser Leu Val Tyr Asn Pro Leu Tyr Ile Phe 
                245                 250                 255     


Arg Met Gln His Asn Asp Thr Ile Pro Glu Ser Val Thr Ala Met Arg 
            260                 265                 270         


Glu Asn Gly Phe Leu Arg Arg Tyr Arg Thr Leu Ala Phe Val Met Arg 
        275                 280                 285             


Ala Phe Phe Ile Phe Arg Thr Ala Phe Leu Pro Trp Tyr Leu Thr Gly 
    290                 295                 300                 


Thr Ser Leu Leu Ile Thr Ile Pro Leu Val Pro Thr Ala Thr Gly Ala 
305                 310                 315                 320 


Phe Leu Thr Phe Phe Phe Ile Leu Ser His Asn Phe Asp Gly Ser Glu 
                325                 330                 335     


Arg Ile Pro Asp Lys Asn Cys Lys Val Lys Ser Ser Glu Lys Asp Val 
            340                 345                 350         


Glu Ala Asp Gln Ile Asp Trp Tyr Arg Ala Gln Val Glu Thr Ser Ser 
        355                 360                 365             


Thr Tyr Gly Gly Pro Ile Ala Met Phe Phe Thr Gly Gly Leu Asn Phe 
    370                 375                 380                 


Gln Ile Glu His His Leu Phe Pro Arg Met Ser Ser Trp His Tyr Pro 
385                 390                 395                 400 


Phe Val Gln Gln Ala Val Arg Glu Cys Cys Glu Arg His Gly Val Arg 
                405                 410                 415     


Tyr Val Phe Tyr Pro Thr Ile Val Gly Asn Ile Ile Ser Thr Leu Lys 
            420                 425                 430         


Tyr Met His Lys Val Gly Val Val His Cys Val Lys Asp Ala Gln Asp 
        435                 440                 445             


Ser 
    


<210>  334
<211>  454
<212>  PRT
<213>  Euglena anabaena

<400>  334

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  335
<211>  1362
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  delta-5 desaturase

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  WO 2008/137532
<311>  2008-05-01
<312>  2008-11-13
<313>  (1)..(1362)

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  US-2008-0274521-A1
<311>  2008-04-29
<312>  2008-11-16
<313>  (1)..(1362)

<400>  335
atg gcc acc atc tct ttg act act gag caa ctt tta gaa cac cca gaa         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctg gtt gca att gat ggg gtg ttg tac gat ctc ttc gga ctg gcg aaa         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtg cat cca ggt ggc aac ctc att gaa gcc gcc ggt gcc tcc gac gga        144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gcc ctg ttc tac tcc atg cac cct gga gtg aag cca gag aat tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctg ctg cag caa ttt gcc cga ggc aaa cac gaa cga agc tcg aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gac cca gtg tac acc ttt gac agt ccc ttc gcc cag gat gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

agc gtt cgg gag gtc atg aag ggg cgc aac tgg tac gcc acg ccc ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttt tgg ctg cgg acc gcg ctg atc atc gcg tgc act gcc ata ggc gaa        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tat tgg atc act acc ggg gca gtg atg tgg ggc atc ttc acc ggg        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac agc cag att ggg ttg gcg att caa cac gat gcc tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtg aac gcc ttt ttc gcc tac ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att gga tcc tcc cgc tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cgc cac cac acc tac acc aac cag cat ggc ctg gac ctg gac gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gcg gag ccg ttc att ttg ttc cac tcc tac ccg gca aca aat        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcg tca cga aag tgg tac cat cgg ttc cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ttg ggg atg tat ggt gtg tcg atg gtg tac aat ccg atg tac ttg        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc acg atg cag cac aac gac aca atc cca gag gcc acc tct ctt aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aac cgg cag cgc gcc ttc gcc gtt tcc ctc cgc        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

cta ctg ttc atc ttc cgc aac gcc ttc ctc ccc tgg tac atc gcg ggc        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct ccg ctg ctc acc atc ctg ctg gtg cca acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ttg aca ttt gtt ttt gtg ctg tcc cat aac ttt gaa ggc gct gag       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc ccc gaa aag aac tgc aag gcc aaa agg gcc aag gag ggg aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc cgc gat gta gag gag gac cgg gtg gac tgg tac cgg gcg cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag acc gcg gcg acc tac ggg ggc agc gtc ggg atg atg ctg acc       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ttg aac ctg cag atc gag cac cac ttg ttc ccc cgc atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tac ccc ttc atc caa gat acg gtg cgg gaa tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cgc cat ggc gtg cgc tac aca tac tac ccg acc atc ctg gag aat ata       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc acg ctc cgc tac atg cag aag gtg ggc gtg gcc cac aca att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gaa ttc                                               1362
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  336
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  336

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  337
<211>  1362
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(1362)
<223>  synthetic delta-5 desaturase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  WO 2008/137532
<311>  2008-05-01
<312>  2008-11-13
<313>  (1)..(1362)

<300>
<302>  DELTA-5 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY
        ACIDS
<310>  US-2008-0274521-A1
<311>  2008-04-29
<312>  2008-11-06
<313>  (1)..(1362)

<400>  337
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtg cat cca gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga        144
Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat gct tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gag ttc                                               1362
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  338
<211>  454
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  338

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Pro Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  339
<211>  8357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEaD5S-A(S)

<400>  339
catggccacc atctccctga ctaccgagca gctcctggaa caccccgagc tcgttgccat       60

cgacggagtc ctgtacgatc tcttcggtct ggccaaggtc catgccggag gcaacctcat      120

cgaagctgcc ggtgcatccg acggaaccgc tctgttctac tccatgcatc ctggagtcaa      180

gccagagaac tcgaagcttc tgcagcaatt tgcccgaggc aagcacgaac gaagctccaa      240

ggatcccgtg tacaccttcg actctccctt tgctcaggac gtcaagcagt ccgttcgaga      300

ggtcatgaag ggtcgaaact ggtacgccac tcctggcttc tggctgagaa ccgcactcat      360

catcgcttgt actgccattg gcgagtggta ctggatcaca accggagcag tgatgtgggg      420

tatctttact ggatacttcc actcgcagat tggcttggcc attcaacacg atgcttctca      480

cggagccatc agcaaaaagc cctgggtcaa cgcctttttc gcttatggca tcgacgccat      540

tggttcctct cgttggatct ggctgcagtc ccacattatg cgacatcaca cttacaccaa      600

ccagcatggc ctcgacctgg atgctgcctc ggcagagccg ttcatcttgt tccactccta      660

tcctgctacc aacgcctctc gaaagtggta ccaccgattt caggcgtggt acatgtacat      720

cgttctggga atgtatggtg tctcgatggt gtacaatccc atgtacctct tcacaatgca      780

gcacaacgac accattcccg aggccacttc tctcagacca ggcagctttt tcaatcggca      840

gcgagctttc gccgtttccc ttcgactgct cttcatcttc cgaaacgcct ttcttccctg      900

gtacattgct ggtgcctctc ctctgctcac cattcttctg gtgcccacgg tcacaggcat      960

cttcctcacc tttgtgttcg ttctgtccca taacttcgag ggagccgaac ggaccccaga     1020

gaagaactgc aaggccaaac gagctaagga aggcaaggag gtcagagacg tggaagagga     1080

tcgagtcgac tggtaccgag cacaggccga gactgctgcc acctacggtg gcagcgtggg     1140

aatgatgctt acaggcggtc tcaacctgca gatcgagcat cacttgtttc cccgaatgtc     1200

ctcttggcac tatcccttca ttcaagacac cgttcgggag tgttgcaagc gacatggcgt     1260

ccgttacaca tactatccta ccattctcga gaacatcatg tccactcttc gatacatgca     1320

gaaggtgggt gttgctcaca ccattcagga tgcccaggag ttctaagcgg ccgcaagtgt     1380

ggatggggaa gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt     1440

caacacaggg atatagcgag ctacgtggtg gtgcgaggat atagcaacgg atatttatgt     1500

ttgacacttg agaatgtacg atacaagcac tgtccaagta caatactaaa catactgtac     1560

atactcatac tcgtacccgg gcaacggttt cacttgagtg cagtggctag tgctcttact     1620

cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta     1680

gttgcgtacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac     1740

tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc     1800

tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg     1860

cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc     1920

actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt     1980

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     2040

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     2100

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     2160

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     2220

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     2280

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     2340

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     2400

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     2460

acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg     2520

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     2580

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     2640

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     2700

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     2760

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     2820

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     2880

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     2940

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     3000

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     3060

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg     3120

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     3180

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg     3240

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt     3300

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt     3360

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata     3420

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc     3480

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac     3540

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa     3600

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct     3660

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat     3720

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc     3780

cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg     3840

tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc     3900

tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc     3960

gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta     4020

gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta     4080

atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg     4140

atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa     4200

aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct     4260

gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa     4320

agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg     4380

ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg     4440

gccccccctc gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt cacacaaacc     4500

gatcttcgcc tcaaggaaac ctaattctac atccgagaga ctgccgagat ccagtctaca     4560

ctgattaatt ttcgggccaa taatttaaaa aaatcgtgtt atataatatt atatgtatta     4620

tatatataca tcatgatgat actgacagtc atgtcccatt gctaaataga cagactccat     4680

ctgccgcctc caactgatgt tctcaatatt taaggggtca tctcgcattg tttaataata     4740

aacagactcc atctaccgcc tccaaatgat gttctcaaaa tatattgtat gaacttattt     4800

ttattactta gtattattag acaacttact tgctttatga aaaacacttc ctatttagga     4860

aacaatttat aatggcagtt cgttcattta acaatttatg tagaataaat gttataaatg     4920

cgtatgggaa atcttaaata tggatagcat aaatgatatc tgcattgcct aattcgaaat     4980

caacagcaac gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa tatcaactat     5040

caaagaacag ctattcacac gttactattg agattattat tggacgagaa tcacacactc     5100

aactgtcttt ctctcttcta gaaatacagg tacaagtatg tactattctc attgttcata     5160

cttctagtca tttcatccca catattcctt ggatttctct ccaatgaatg acattctatc     5220

ttgcaaattc aacaattata ataagatata ccaaagtagc ggtatagtgg caatcaaaaa     5280

gcttctctgg tgtgcttctc gtatttattt ttattctaat gatccattaa aggtatatat     5340

ttatttcttg ttatataatc cttttgttta ttacatgggc tggatacata aaggtatttt     5400

gatttaattt tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt aatggtagga     5460

aattaccata cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg tatttccagg     5520

ttagacgttc cgcagaatct agaatgcggt atgcggtaca ttgttcttcg aacgtaaaag     5580

ttgcgctccc tgagatattg tacatttttg cttttacaag tacaagtaca tcgtacaact     5640

atgtactact gttgatgcat ccacaacagt ttgttttgtt tttttttgtt tttttttttt     5700

ctaatgattc attaccgcta tgtataccta cttgtacttg tagtaagccg ggttattggc     5760

gttcaattaa tcatagactt atgaatctgc acggtgtgcg ctgcgagtta cttttagctt     5820

atgcatgcta cttgggtgta atattgggat ctgttcggaa atcaacggat gctcaatcga     5880

tttcgacagt aattaattaa gtcatacaca agtcagcttt cttcgagcct catataagta     5940

taagtagttc aacgtattag cactgtaccc agcatctccg tatcgagaaa cacaacaaca     6000

tgccccattg gacagatcat gcggatacac aggttgtgca gtatcataca tactcgatca     6060

gacaggtcgt ctgaccatca tacaagctga acaagcgctc catacttgca cgctctctat     6120

atacacagtt aaattacata tccatagtct aacctctaac agttaatctt ctggtaagcc     6180

tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt ctggccgtac     6240

agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc aacagttcgg     6300

tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt cagaataagc     6360

cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac aaactcgggg     6420

tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag agagcccttg     6480

caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg agaggggact     6540

aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt ctgttcagag     6600

acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg tacaccgtgg     6660

gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg cttgacagtg     6720

ttgccaatat ctgcgaactt tctgtcctcg aacaggaaga aaccgtgctt aagagcaagt     6780

tccttgaggg ggagcacagt gccggcgtag gtgaagtcgt caatgatgtc gatatgggtt     6840

ttgatcatgc acacataagg tccgacctta tcggcaagct caatgagctc cttggtggtg     6900

gtaacatcca gagaagcaca caggttggtt ttcttggctg ccacgagctt gagcactcga     6960

gcggcaaagg cggacttgtg gacgttagct cgagcttcgt aggagggcat tttggtggtg     7020

aagaggagac tgaaataaat ttagtctgca gaacttttta tcggaacctt atctggggca     7080

gtgaagtata tgttatggta atagttacga gttagttgaa cttatagata gactggacta     7140

tacggctatc ggtccaaatt agaaagaacg tcaatggctc tctgggcgtc gcctttgccg     7200

acaaaaatgt gatcatgatg aaagccagca atgacgttgc agctgatatt gttgtcggcc     7260

aaccgcgccg aaaacgcagc tgtcagaccc acagcctcca acgaagaatg tatcgtcaaa     7320

gtgatccaag cacactcata gttggagtcg tactccaaag gcggcaatga cgagtcagac     7380

agatactcgt cgacgtttaa acagtgtacg cagatctact atagaggaac atttaaattg     7440

ccccggagaa gacggccagg ccgcctagat gacaaattca acaactcaca gctgactttc     7500

tgccattgcc actagggggg ggccttttta tatggccaag ccaagctctc cacgtcggtt     7560

gggctgcacc caacaataaa tgggtagggt tgcaccaaca aagggatggg atggggggta     7620

gaagatacga ggataacggg gctcaatggc acaaataaga acgaatactg ccattaagac     7680

tcgtgatcca gcgactgaca ccattgcatc atctaagggc ctcaaaacta cctcggaact     7740

gctgcgctga tctggacacc acagaggttc cgagcacttt aggttgcacc aaatgtccca     7800

ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt tgtcttaaca aaaagtgagg     7860

gcgctgaggt cgagcagggt ggtgtgactt gttatagcct ttagagctgc gaaagcgcgt     7920

atggatttgg ctcatcaggc cagattgagg gtctgtggac acatgtcatg ttagtgtact     7980

tcaatcgccc cctggatata gccccgacaa taggccgtgg cctcattttt ttgccttccg     8040

cacatttcca ttgctcgata cccacacctt gcttctcctg cacttgccaa ccttaatact     8100

ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc tagggtatat ataaacagtg     8160

gctctcccaa tcggttgcca gtctcttttt tcctttcttt ccccacagat tcgaaatcta     8220

aactacacat cacagaattc cgagccgtga gtatccacga caagatcagt gtcgagacga     8280

cgcgttttgt gtaatgacac aatccgaaag tcgctagcaa cacacactct ctacacaaac     8340

taacccagct ctggtac                                                    8357


<210>  340
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  340
ttggccattc aacacgaggc ttctcacgga gc                                     32


<210>  341
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  341
gctccgtgag aagcctcgtg ttgaatggcc aa                                     32


<210>  342
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  342
gattggcttg gccattcaac acgatttctc tcacggagcc atca                        44


<210>  343
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  343
tgatggctcc gtgagagaaa tcgtgttgaa tggccaagcc aatc                        44


<210>  344
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  344
gccattcaac acgatggttc tcacggagcc atc                                    33


<210>  345
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  345
gatggctccg tgagaaccat cgtgttgaat ggc                                    33


<210>  346
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  346
gattggcttg gccattcaac acgatatgtc tcacggagcc atca                        44


<210>  347
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  347
tgatggctcc gtgagacata tcgtgttgaa tggccaagcc aatc                        44


<210>  348
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  348
ggcttggcca ttcaacacga ttcctctcac ggagcca                                37


<210>  349
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  349
tggctccgtg agaggaatcg tgttgaatgg ccaagcc                                37


<210>  350
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  350
gattggcttg gccattcaac acgattactc tcacggagcc atca                        44


<210>  351
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  351
tgatggctcc gtgagagtaa tcgtgttgaa tggccaagcc aatc                        44


<210>  352
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  352
ccattcaaca cgatgctgcc cacggagcca tcagcaa                                37


<210>  353
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  353
ttgctgatgg ctccgtgggc agcatcgtgt tgaatgg                                37


<210>  354
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  354
ggccattcaa cacgatgctt gtcacggagc ca                                     32


<210>  355
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  355
tggctccgtg acaagcatcg tgttgaatgg cc                                     32


<210>  356
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  356
cttggccatt caacacgatg ctggtcacgg agccat                                 36


<210>  357
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  357
atggctccgt gaccagcatc gtgttgaatg gccaag                                 36


<210>  358
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  358
ttggccattc aacacgatgc taaccacgga gccatcagca aaaag                       45


<210>  359
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  359
ctttttgctg atggctccgt ggttagcatc gtgttgaatg gccaa                       45


<210>  360
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  360
ccattcaaca cgatgctacc cacggagcca tcagcaa                                37


<210>  361
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  361
ttgctgatgg ctccgtgggt agcatcgtgt tgaatgg                                37


<210>  362
<211>  1365
<212>  DNA
<213>  Euglena anabaena


<220>
<221>  CDS
<222>  (1)..(1365)

<400>  362
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtc cat gcc gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga        144
Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat tcc tct cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ser Ser His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gag ttc taa                                           1365
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  363
<211>  454
<212>  PRT
<213>  Euglena anabaena

<400>  363

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ser Ser His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  364
<211>  1365
<212>  DNA
<213>  Euglena anabaena


<220>
<221>  CDS
<222>  (1)..(1365)

<400>  364
atg gcc acc atc tcc ctg act acc gag cag ctc ctg gaa cac ccc gag         48
Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu           
1               5                   10                  15                

ctc gtt gcc atc gac gga gtc ctg tac gat ctc ttc ggt ctg gcc aag         96
Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys           
            20                  25                  30                    

gtc cat gcc gga ggc aac ctc atc gaa gct gcc ggt gca tcc gac gga        144
Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly           
        35                  40                  45                        

acc gct ctg ttc tac tcc atg cat cct gga gtc aag cca gag aac tcg        192
Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser           
    50                  55                  60                            

aag ctt ctg cag caa ttt gcc cga ggc aag cac gaa cga agc tcc aag        240
Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys           
65                  70                  75                  80            

gat ccc gtg tac acc ttc gac tct ccc ttt gct cag gac gtc aag cag        288
Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln           
                85                  90                  95                

tcc gtt cga gag gtc atg aag ggt cga aac tgg tac gcc act cct ggc        336
Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly           
            100                 105                 110                   

ttc tgg ctg aga acc gca ctc atc atc gct tgt act gcc att ggc gag        384
Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu           
        115                 120                 125                       

tgg tac tgg atc aca acc gga gca gtg atg tgg ggt atc ttt act gga        432
Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly           
    130                 135                 140                           

tac ttc cac tcg cag att ggc ttg gcc att caa cac gat gct ggt cac        480
Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Gly His           
145                 150                 155                 160           

gga gcc atc agc aaa aag ccc tgg gtc aac gcc ttt ttc gct tat ggc        528
Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly           
                165                 170                 175               

atc gac gcc att ggt tcc tct cgt tgg atc tgg ctg cag tcc cac att        576
Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile           
            180                 185                 190                   

atg cga cat cac act tac acc aac cag cat ggc ctc gac ctg gat gct        624
Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala           
        195                 200                 205                       

gcc tcg gca gag ccg ttc atc ttg ttc cac tcc tat cct gct acc aac        672
Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn           
    210                 215                 220                           

gcc tct cga aag tgg tac cac cga ttt cag gcg tgg tac atg tac atc        720
Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile           
225                 230                 235                 240           

gtt ctg gga atg tat ggt gtc tcg atg gtg tac aat ccc atg tac ctc        768
Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu           
                245                 250                 255               

ttc aca atg cag cac aac gac acc att ccc gag gcc act tct ctc aga        816
Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg           
            260                 265                 270                   

cca ggc agc ttt ttc aat cgg cag cga gct ttc gcc gtt tcc ctt cga        864
Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg           
        275                 280                 285                       

ctg ctc ttc atc ttc cga aac gcc ttt ctt ccc tgg tac att gct ggt        912
Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly           
    290                 295                 300                           

gcc tct cct ctg ctc acc att ctt ctg gtg ccc acg gtc aca ggc atc        960
Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile           
305                 310                 315                 320           

ttc ctc acc ttt gtg ttc gtt ctg tcc cat aac ttc gag gga gcc gaa       1008
Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu           
                325                 330                 335               

cgg acc cca gag aag aac tgc aag gcc aaa cga gct aag gaa ggc aag       1056
Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys           
            340                 345                 350                   

gag gtc aga gac gtg gaa gag gat cga gtc gac tgg tac cga gca cag       1104
Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln           
        355                 360                 365                       

gcc gag act gct gcc acc tac ggt ggc agc gtg gga atg atg ctt aca       1152
Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr           
    370                 375                 380                           

ggc ggt ctc aac ctg cag atc gag cat cac ttg ttt ccc cga atg tcc       1200
Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser           
385                 390                 395                 400           

tct tgg cac tat ccc ttc att caa gac acc gtt cgg gag tgt tgc aag       1248
Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys           
                405                 410                 415               

cga cat ggc gtc cgt tac aca tac tat cct acc att ctc gag aac atc       1296
Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile           
            420                 425                 430                   

atg tcc act ctt cga tac atg cag aag gtg ggt gtt gct cac acc att       1344
Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile           
        435                 440                 445                       

cag gat gcc cag gag ttc taa                                           1365
Gln Asp Ala Gln Glu Phe                                                   
    450                                                                   


<210>  365
<211>  454
<212>  PRT
<213>  Euglena anabaena

<400>  365

Met Ala Thr Ile Ser Leu Thr Thr Glu Gln Leu Leu Glu His Pro Glu 
1               5                   10                  15      


Leu Val Ala Ile Asp Gly Val Leu Tyr Asp Leu Phe Gly Leu Ala Lys 
            20                  25                  30          


Val His Ala Gly Gly Asn Leu Ile Glu Ala Ala Gly Ala Ser Asp Gly 
        35                  40                  45              


Thr Ala Leu Phe Tyr Ser Met His Pro Gly Val Lys Pro Glu Asn Ser 
    50                  55                  60                  


Lys Leu Leu Gln Gln Phe Ala Arg Gly Lys His Glu Arg Ser Ser Lys 
65                  70                  75                  80  


Asp Pro Val Tyr Thr Phe Asp Ser Pro Phe Ala Gln Asp Val Lys Gln 
                85                  90                  95      


Ser Val Arg Glu Val Met Lys Gly Arg Asn Trp Tyr Ala Thr Pro Gly 
            100                 105                 110         


Phe Trp Leu Arg Thr Ala Leu Ile Ile Ala Cys Thr Ala Ile Gly Glu 
        115                 120                 125             


Trp Tyr Trp Ile Thr Thr Gly Ala Val Met Trp Gly Ile Phe Thr Gly 
    130                 135                 140                 


Tyr Phe His Ser Gln Ile Gly Leu Ala Ile Gln His Asp Ala Gly His 
145                 150                 155                 160 


Gly Ala Ile Ser Lys Lys Pro Trp Val Asn Ala Phe Phe Ala Tyr Gly 
                165                 170                 175     


Ile Asp Ala Ile Gly Ser Ser Arg Trp Ile Trp Leu Gln Ser His Ile 
            180                 185                 190         


Met Arg His His Thr Tyr Thr Asn Gln His Gly Leu Asp Leu Asp Ala 
        195                 200                 205             


Ala Ser Ala Glu Pro Phe Ile Leu Phe His Ser Tyr Pro Ala Thr Asn 
    210                 215                 220                 


Ala Ser Arg Lys Trp Tyr His Arg Phe Gln Ala Trp Tyr Met Tyr Ile 
225                 230                 235                 240 


Val Leu Gly Met Tyr Gly Val Ser Met Val Tyr Asn Pro Met Tyr Leu 
                245                 250                 255     


Phe Thr Met Gln His Asn Asp Thr Ile Pro Glu Ala Thr Ser Leu Arg 
            260                 265                 270         


Pro Gly Ser Phe Phe Asn Arg Gln Arg Ala Phe Ala Val Ser Leu Arg 
        275                 280                 285             


Leu Leu Phe Ile Phe Arg Asn Ala Phe Leu Pro Trp Tyr Ile Ala Gly 
    290                 295                 300                 


Ala Ser Pro Leu Leu Thr Ile Leu Leu Val Pro Thr Val Thr Gly Ile 
305                 310                 315                 320 


Phe Leu Thr Phe Val Phe Val Leu Ser His Asn Phe Glu Gly Ala Glu 
                325                 330                 335     


Arg Thr Pro Glu Lys Asn Cys Lys Ala Lys Arg Ala Lys Glu Gly Lys 
            340                 345                 350         


Glu Val Arg Asp Val Glu Glu Asp Arg Val Asp Trp Tyr Arg Ala Gln 
        355                 360                 365             


Ala Glu Thr Ala Ala Thr Tyr Gly Gly Ser Val Gly Met Met Leu Thr 
    370                 375                 380                 


Gly Gly Leu Asn Leu Gln Ile Glu His His Leu Phe Pro Arg Met Ser 
385                 390                 395                 400 


Ser Trp His Tyr Pro Phe Ile Gln Asp Thr Val Arg Glu Cys Cys Lys 
                405                 410                 415     


Arg His Gly Val Arg Tyr Thr Tyr Tyr Pro Thr Ile Leu Glu Asn Ile 
            420                 425                 430         


Met Ser Thr Leu Arg Tyr Met Gln Lys Val Gly Val Ala His Thr Ile 
        435                 440                 445             


Gln Asp Ala Gln Glu Phe 
    450                 


<210>  366
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer FBAIN-F

<400>  366
caagcggggg gcttgtctag ggtat                                             25


<210>  367
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Y1026

<400>  367
tttgcggccg ctcactgaat ctttttggct cccttgtgct                             40


<210>  368
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G/W132T/I179R"

<400>  368
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc acc ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  369
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  369

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  370
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-L35G/W132T/I179R

<400>  370
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

accaccttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  371
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-S9D/L35G/Y84C/I179R"

<400>  371
atg gag gtc gtg aac gaa atc gtc gac att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Asp Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tgt acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  372
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  372

Met Glu Val Val Asn Glu Ile Val Asp Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  373
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-S9D/L35G/Y84C/I179R

<400>  373
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtcgacatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt gtaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  374
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G/Y84C/I179R/Q244N"

<400>  374
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tgt acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg aac acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  375
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  375

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  376
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-L35G/Y84C/I179R/Q244N

<400>  376
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt gtaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtga acacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  377
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-A21V/L35G/W132T/I179R/Q244N"

<400>  377
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gtg cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc acc ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg aac acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  378
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  378

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  379
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-A21V/L35G/W132T/I179R/Q244N

<400>  379
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gtgcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

accaccttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtga acacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  380
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDGNH motif

<400>  380

His Asp Gly Asn His 
1               5   


<210>  381
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDANH motif

<400>  381

His Asp Ala Asn His 
1               5   


<210>  382
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HEFAH motif

<400>  382

His Glu Phe Ala His 
1               5   


<210>  383
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HEFTH motif

<400>  383

His Glu Phe Thr His 
1               5   


<210>  384
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HEMGH motif

<400>  384

His Glu Met Gly His 
1               5   


<210>  385
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HEAGH motif

<400>  385

His Glu Ala Gly His 
1               5   


<210>  386
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HDFGH motif

<400>  386

His Asp Phe Gly His 
1               5   


<210>  387
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDYGH motif

<400>  387

His Asp Tyr Gly His 
1               5   


<210>  388
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDSCH motif

<400>  388

His Asp Ser Cys His 
1               5   


<210>  389
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDACH motif

<400>  389

His Asp Ala Cys His 
1               5   


<210>  390
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDcSH motif

<400>  390

His Asp Cys Ser His 
1               5   


<210>  391
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDdSH motif

<400>  391

His Asp Asp Ser His 
1               5   


<210>  392
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDeSH motif

<400>  392

His Asp Glu Ser His 
1               5   


<210>  393
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDfSH motif

<400>  393

His Asp Phe Ser His 
1               5   


<210>  394
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDhSH motif

<400>  394

His Asp His Ser His 
1               5   


<210>  395
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDiSH motif

<400>  395

His Asp Ile Ser His 
1               5   


<210>  396
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDkSH motif

<400>  396

His Asp Lys Ser His 
1               5   


<210>  397
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDlSH motif

<400>  397

His Asp Leu Ser His 
1               5   


<210>  398
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDmSH motif

<400>  398

His Asp Met Ser His 
1               5   


<210>  399
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDnSH motif

<400>  399

His Asp Asn Ser His 
1               5   


<210>  400
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDpSH motif

<400>  400

His Asp Pro Ser His 
1               5   


<210>  401
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDqSH motif

<400>  401

His Asp Gln Ser His 
1               5   


<210>  402
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDrSH motif

<400>  402

His Asp Arg Ser His 
1               5   


<210>  403
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDtSH motif

<400>  403

His Asp Thr Ser His 
1               5   


<210>  404
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDvSH motif

<400>  404

His Asp Val Ser His 
1               5   


<210>  405
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDwSH motif

<400>  405

His Asp Trp Ser His 
1               5   


<210>  406
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDySH motif

<400>  406

His Asp Tyr Ser His 
1               5   


<210>  407
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAcH motif

<400>  407

His Asp Ala Cys His 
1               5   


<210>  408
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAdH motif

<400>  408

Val His Asp Ala Asp His 
1               5       


<210>  409
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAeH motif

<400>  409

His Asp Ala Glu His 
1               5   


<210>  410
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAfH motif

<400>  410

His Asp Ala Phe His 
1               5   


<210>  411
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAhH motif

<400>  411

His Asp Ala His His 
1               5   


<210>  412
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAiH motif

<400>  412

His Asp Ala Ile His 
1               5   


<210>  413
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAkH motif

<400>  413

His Asp Ala Lys His 
1               5   


<210>  414
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAlH motif

<400>  414

His Asp Ala Leu His 
1               5   


<210>  415
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAmH motif

<400>  415

His Asp Ala Met His 
1               5   


<210>  416
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAnH motif

<400>  416

His Asp Ala Asn His 
1               5   


<210>  417
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDApH motif

<400>  417

His Asp Ala Pro His 
1               5   


<210>  418
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAqH motif

<400>  418

His Asp Ala Gln His 
1               5   


<210>  419
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDArH motif

<400>  419

His Asp Ala Arg His 
1               5   


<210>  420
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAtH motif

<400>  420

His Asp Ala Thr His 
1               5   


<210>  421
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAvH motif

<400>  421

His Asp Ala Val His 
1               5   


<210>  422
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAwH motif

<400>  422

His Asp Ala Trp His 
1               5   


<210>  423
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAyH motif

<400>  423

His Asp Ala Tyr His 
1               5   


<210>  424
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDxxH motif


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  424

His Asp Xaa Xaa His 
1               5   


<210>  425
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HGGG motif

<400>  425

His Gly Gly Gly 
1               


<210>  426
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HHGG motif

<400>  426

His His Gly Gly 
1               


<210>  427
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HPGS motif

<400>  427

His Pro Gly Ser 
1               


<210>  428
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HAGG motif

<400>  428

His Ala Gly Gly 
1               


<210>  429
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDGSH motif

<400>  429

His Asp Gly Ser His 
1               5   


<210>  430
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDSSH motif

<400>  430

His Asp Ser Ser His 
1               5   


<210>  431
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAAH motif

<400>  431

His Asp Ala Ala His 
1               5   


<210>  432
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAGH motif

<400>  432

His Asp Ala Gly His 
1               5   


<210>  433
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HEASH motif

<400>  433

His Glu Ala Ser His 
1               5   


<210>  434
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDXSH motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  434

His Asp Xaa Ser His 
1               5   


<210>  435
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HDAXH motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  435

His Asp Ala Xaa His 
1               5   


