                         SEQUENCE LISTING

<110>  E.I. duPont de Nemours and Company, Inc.
       Li, Yougen
       Bostick, Michael
       Zhu, Quinn
 
<120>  MUTANT DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED

<130>  CL4783

<150>  US 61/377,248
<151>  2010-08-26

<160>  111   

<170>  PatentIn version 3.5

<210>  1
<211>  1064
<212>  DNA
<213>  Isochrysis galbana (GenBank Accession No. AF390174)


<220>
<221>  CDS
<222>  (2)..(793)
<223>  delta-9 elongase (IgD9e)

<400>  1
g atg gcc ctc gca aac gac gcg gga gag cgc atc tgg gcg gct gtg acc       49
  Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr         
  1               5                   10                  15              

gac ccg gaa atc ctc att ggc acc ttc tcg tac ttg cta ctc aaa ccg         97
Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro           
            20                  25                  30                    

ctg ctc cgc aat tcc ggg ctg gtg gat gag aag aag ggc gca tac agg        145
Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg           
        35                  40                  45                        

acg tcc atg atc tgg tac aac gtt ctg ctg gcg ctc ttc tct gcg ctg        193
Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu           
    50                  55                  60                            

agc ttc tac gtg acg gcg acc gcc ctc ggc tgg gac tat ggt acg ggc        241
Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly           
65                  70                  75                  80            

gcg tgg ctg cgc agg caa acc ggc gac aca ccg cag ccg ctc ttc cag        289
Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln           
                85                  90                  95                

tgc ccg tcc ccg gtt tgg gac tcg aag ctc ttc aca tgg acc gcc aag        337
Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys           
            100                 105                 110                   

gca ttc tat tac tcc aag tac gtg gag tac ctc gac acg gcc tgg ctg        385
Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu           
        115                 120                 125                       

gtg ctc aag ggc aag agg gtc tcc ttt ctc cag gcc ttc cac cac ttt        433
Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe           
    130                 135                 140                           

ggc gcg ccg tgg gat gtg tac ctc ggc att cgg ctg cac aac gag ggc        481
Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly           
145                 150                 155                 160           

gta tgg atc ttc atg ttt ttc aac tcg ttc att cac acc atc atg tac        529
Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr           
                165                 170                 175               

acc tac tac ggc ctc acc gcc gcc ggg tat aag ttc aag gcc aag ccg        577
Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro           
            180                 185                 190                   

ctc atc acc gcg atg cag atc tgc cag ttc gtg ggc ggc ttc ctg ttg        625
Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu           
        195                 200                 205                       

gtc tgg gac tac atc aac gtc ccc tgc ttc aac tcg gac aaa ggg aag        673
Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys           
    210                 215                 220                           

ttg ttc agc tgg gct ttc aac tat gca tac gtc ggc tcg gtc ttc ttg        721
Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu           
225                 230                 235                 240           

ctc ttc tgc cac ttt ttc tac cag gac aac ttg gca acg aag aaa tcg        769
Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser           
                245                 250                 255               

gcc aag gcg ggc aag cag ctc tag gcctcgagcc ggctcgcggg ttcaaggagg       823
Ala Lys Ala Gly Lys Gln Leu                                               
            260                                                           

gcgacacggg ggtgggacgt ttgcatggag atggattgtg gatgtcctta cgccttactc      883

atcaatgtcc tcccatctct cccctctaga ccttctacta gccatctaga agggcagctc      943

agagacggat accgttcccc ctccccttcc ttttcgtctt tgctttgcca ttgtttgttt     1003

gtctctattt tttaaactat tgacgctaac gcgttacgct cgcaaaaaaa aaaaaaaaaa     1063

a                                                                     1064


<210>  2
<211>  263
<212>  PRT
<213>  Isochrysis galbana (GenBank Accession No. AF390174)

<400>  2

Met Ala Leu Ala Asn Asp Ala Gly Glu Arg Ile Trp Ala Ala Val Thr 
1               5                   10                  15      


Asp Pro Glu Ile Leu Ile Gly Thr Phe Ser Tyr Leu Leu Leu Lys Pro 
            20                  25                  30          


Leu Leu Arg Asn Ser Gly Leu Val Asp Glu Lys Lys Gly Ala Tyr Arg 
        35                  40                  45              


Thr Ser Met Ile Trp Tyr Asn Val Leu Leu Ala Leu Phe Ser Ala Leu 
    50                  55                  60                  


Ser Phe Tyr Val Thr Ala Thr Ala Leu Gly Trp Asp Tyr Gly Thr Gly 
65                  70                  75                  80  


Ala Trp Leu Arg Arg Gln Thr Gly Asp Thr Pro Gln Pro Leu Phe Gln 
                85                  90                  95      


Cys Pro Ser Pro Val Trp Asp Ser Lys Leu Phe Thr Trp Thr Ala Lys 
            100                 105                 110         


Ala Phe Tyr Tyr Ser Lys Tyr Val Glu Tyr Leu Asp Thr Ala Trp Leu 
        115                 120                 125             


Val Leu Lys Gly Lys Arg Val Ser Phe Leu Gln Ala Phe His His Phe 
    130                 135                 140                 


Gly Ala Pro Trp Asp Val Tyr Leu Gly Ile Arg Leu His Asn Glu Gly 
145                 150                 155                 160 


Val Trp Ile Phe Met Phe Phe Asn Ser Phe Ile His Thr Ile Met Tyr 
                165                 170                 175     


Thr Tyr Tyr Gly Leu Thr Ala Ala Gly Tyr Lys Phe Lys Ala Lys Pro 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Cys Gln Phe Val Gly Gly Phe Leu Leu 
        195                 200                 205             


Val Trp Asp Tyr Ile Asn Val Pro Cys Phe Asn Ser Asp Lys Gly Lys 
    210                 215                 220                 


Leu Phe Ser Trp Ala Phe Asn Tyr Ala Tyr Val Gly Ser Val Phe Leu 
225                 230                 235                 240 


Leu Phe Cys His Phe Phe Tyr Gln Asp Asn Leu Ala Thr Lys Lys Ser 
                245                 250                 255     


Ala Lys Ala Gly Lys Gln Leu 
            260             


<210>  3
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(792)

<400>  3
atg gct gcg gtg ata gag gtc gcc aac gag ttt gta gcc atc acg gca         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gaa acg ctc ccc aaa gtt gac tat caa cga cta tgg cga gac att tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

agt tgt gag cta ctg tat ttc tcc att gcc ttc gtg atc ttg aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acg ttg ggc gag ttg agc gac agc gga aaa aag att ttg aga gtg ttg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aat ctc ttc atg tcc gtg ttc tcc ttg gtg tct ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctt tgc atg ggc tat gcc att tat acc gtg ggc cta tac tct aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac agg gct ttc gac aac tcg ttg ttc cgc ttt gca aca aag gtg        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tac agt aag ttt ttg gag tac atc gac tct ttt tat ctt ccg        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag ccg ctg tct ttc ctg caa ttc ttc cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gcc ccc atg gac atg tgg ctc ttt gtc caa tat tct ggg gaa tct att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttt ttg aat ggg ttc att cac ttt gtt atg tac ggg        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tac tgg act cgg ctg atg aag ttc aat ttc cca atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ttg att acc gcg atg cag atc acg cag ttc aac gtt ggt ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aaa gat att ccc tgc tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ttg gcc tgg atc ttc aat tac tgg tat gtt ggg act gtc ttg ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctg ttc att aat ttc ttc gtc aaa tcc tat gtg ttc cca aag ccg aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gca gat aaa aag gtc caa tag                                        792
Thr Ala Asp Lys Lys Val Gln                                               
            260                                                           


<210>  4
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  4

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  5
<211>  792
<212>  DNA
<213>  Eutreptiella sp. CCMP389


<220>
<221>  CDS
<222>  (1)..(792)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(792)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(792)

<400>  5
atg gct gcc gtc atc gag gtg gcc aac gag ttc gtc gct atc act gcc         48
Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala           
1               5                   10                  15                

gag acc ctt ccc aag gtg gac tat cag cga ctc tgg cga gac atc tac         96
Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr           
            20                  25                  30                    

tcc tgc gag ctc ctg tac ttc tcc att gct ttc gtc atc ctc aag ttt        144
Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe           
        35                  40                  45                        

acc ctt ggc gag ctc tcg gat tct ggc aaa aag att ctg cga gtg ctg        192
Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu           
    50                  55                  60                            

ttc aag tgg tac aac ctc ttc atg tcc gtc ttt tcg ctg gtg tcc ttc        240
Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe           
65                  70                  75                  80            

ctc tgt atg ggt tac gcc atc tac acc gtt gga ctg tac tcc aac gaa        288
Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu           
                85                  90                  95                

tgc gac aga gct ttc gac aac agc ttg ttc cga ttt gcc acc aag gtc        336
Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val           
            100                 105                 110                   

ttc tac tat tcc aag ttt ctg gag tac atc gac tct ttc tac ctt ccc        384
Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro           
        115                 120                 125                       

ctc atg gcc aag cct ctg tcc ttt ctg cag ttc ttt cat cac ttg gga        432
Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly           
    130                 135                 140                           

gct cct atg gac atg tgg ctc ttc gtg cag tac tct ggc gaa tcc att        480
Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile           
145                 150                 155                 160           

tgg atc ttt gtg ttc ctg aac gga ttc att cac ttt gtc atg tac ggc        528
Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly           
                165                 170                 175               

tac tat tgg aca cgg ctg atg aag ttc aac ttt ccc atg ccc aag cag        576
Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln           
            180                 185                 190                   

ctc att acc gca atg cag atc acc cag ttc aac gtt ggc ttc tac ctc        624
Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu           
        195                 200                 205                       

gtg tgg tgg tac aag gac att ccc tgt tac cga aag gat ccc atg cga        672
Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg           
    210                 215                 220                           

atg ctg gcc tgg atc ttc aac tac tgg tac gtc ggt acc gtt ctt ctg        720
Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu           
225                 230                 235                 240           

ctc ttc atc aac ttc ttt gtc aag tcc tac gtg ttt ccc aag cct aag        768
Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys           
                245                 250                 255               

act gcc gac aaa aag gtc cag tag                                        792
Thr Ala Asp Lys Lys Val Gln                                               
            260                                                           


<210>  6
<211>  263
<212>  PRT
<213>  Eutreptiella sp. CCMP389

<400>  6

Met Ala Ala Val Ile Glu Val Ala Asn Glu Phe Val Ala Ile Thr Ala 
1               5                   10                  15      


Glu Thr Leu Pro Lys Val Asp Tyr Gln Arg Leu Trp Arg Asp Ile Tyr 
            20                  25                  30          


Ser Cys Glu Leu Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe 
        35                  40                  45              


Thr Leu Gly Glu Leu Ser Asp Ser Gly Lys Lys Ile Leu Arg Val Leu 
    50                  55                  60                  


Phe Lys Trp Tyr Asn Leu Phe Met Ser Val Phe Ser Leu Val Ser Phe 
65                  70                  75                  80  


Leu Cys Met Gly Tyr Ala Ile Tyr Thr Val Gly Leu Tyr Ser Asn Glu 
                85                  90                  95      


Cys Asp Arg Ala Phe Asp Asn Ser Leu Phe Arg Phe Ala Thr Lys Val 
            100                 105                 110         


Phe Tyr Tyr Ser Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro 
        115                 120                 125             


Leu Met Ala Lys Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly 
    130                 135                 140                 


Ala Pro Met Asp Met Trp Leu Phe Val Gln Tyr Ser Gly Glu Ser Ile 
145                 150                 155                 160 


Trp Ile Phe Val Phe Leu Asn Gly Phe Ile His Phe Val Met Tyr Gly 
                165                 170                 175     


Tyr Tyr Trp Thr Arg Leu Met Lys Phe Asn Phe Pro Met Pro Lys Gln 
            180                 185                 190         


Leu Ile Thr Ala Met Gln Ile Thr Gln Phe Asn Val Gly Phe Tyr Leu 
        195                 200                 205             


Val Trp Trp Tyr Lys Asp Ile Pro Cys Tyr Arg Lys Asp Pro Met Arg 
    210                 215                 220                 


Met Leu Ala Trp Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu 
225                 230                 235                 240 


Leu Phe Ile Asn Phe Phe Val Lys Ser Tyr Val Phe Pro Lys Pro Lys 
                245                 250                 255     


Thr Ala Asp Lys Lys Val Gln 
            260             


<210>  7
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(777)

<400>  7
atg gag gtg gtg aat gaa ata gtc tca att ggg cag gaa gtt tta ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aaa gtt gat tat gcc caa ctc tgg agt gat gcc agt cac tgt gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctt tac ttg tcc atc gca ttt gtc atc ttg aag ttc act ctt ggc ccc        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctt ggt cca aaa ggt cag tct cgt atg aag ttt gtt ttc acc aat tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctt ctc atg tcc att tat tcg ttg gga tca ttc ctc tca atg gca        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tat gcc atg tac acc atc ggt gtt atg tct gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttt gac aac aac gtc ttc agg atc acc acg cag ttg ttc tat ttg agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctg gag tat att gac tcc ttc tat ttg cca ctg atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg caa ttc ttc cat cat ttg ggg gca ccg atg gat        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tat aat tac cga aat gaa gct gtt tgg att ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ttg aat ggt ttc atc cac tgg atc atg tac ggt tat tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

aga ttg atc aag ctg aag ttc ccc atg cca aaa tcc ctg att aca tca        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att caa ttc aat gtt ggt ttc tac att gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

agg aac att ccc tgt tat cgc caa gat ggg atg agg atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttc ttc aat tac ttt tat gtt ggc aca gtc ttg tgt ttg ttc ttg aat        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tat gtg caa acg tat atc gtc agg aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  8
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  8

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  9
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2007/061742
<311>  2006-11-16
<312>  2007-05-31
<313>  (1)..(777)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(777)

<400>  9
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  10
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  10

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  11
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  delta-9 elongase

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,794,701
<311>  2008-04-15
<312>  2010-09-14
<313>  (1)..(774)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2008/128241
<311>  2008-04-16
<312>  2008-10-23
<313>  (1)..(774)

<400>  11
atg gaa gca gcc aaa gaa ttg gtt tcc atc gtc caa gag gag ctc ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tat gcc cag ctt tgg cag gat gcc agc agc tgt gag gtc         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctt tac ctc tcg gtg gca ttc gtg gcg atc aag ttc atg ctg cgc cca        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctg aag cgc cag gcc acc ttg aag aag ctg ttc aca gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttc ctc atg tcg atc tat tcc ttt ggc tcc ttc ctg gcc atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tat gcc cta tca gta act ggc atc ctc tcc ggc gac tgt gag acg gcg        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aac gat gtg ttc agg atc aca act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gta gag tac atc gac tcc ttc tac ctt ccc ctt atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

cca ctg tcg ttc ctt cag ttc ttc cat cat ttg ggg gcc ccc att gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg cta ttc tac aaa tac cgc aac gaa gga gtc tgg atc ttt gtc        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ttg aat ggg ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cgg ctc atc aag ctg aac ttc ccc atg ccc aag aac ctg atc acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc atc cag ttc aat gtc ggg ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgc aat gtg cca tgc tac cgc cag gat ggg atg cgc atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggg acg gtc ttg ctg ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttt tac gtg cag acg tac atc cgg aag ccg agg aag aac cga ggg aag        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag                                                                774
Lys Glu                                                                   
                                                                          


<210>  12
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  12

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  13
<211>  774
<212>  DNA
<213>  Euglena anabaena UTEX 373


<220>
<221>  CDS
<222>  (1)..(774)
<223>  synthetic delta-9 elongase (codon-optimized for Yarrowia 
       lipolytica)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,794,701
<311>  2008-04-15
<312>  2010-09-14
<313>  (1)..(774)

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  WO 2008/128241
<311>  2008-04-16
<312>  2008-10-23
<313>  (1)..(774)

<400>  13
atg gag gct gcc aag gag ctg gtc tcc atc gtc cag gag gaa ctt ccc         48
Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro           
1               5                   10                  15                

aag gtg gac tac gcc cag ctc tgg cag gac gcc tcc tct tgc gag gtt         96
Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcg gtc gct ttc gtg gcc atc aag ttc atg ctt cga cct        144
Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro           
        35                  40                  45                        

ctg gac ctc aag cga caa gcc acc ctc aaa aag ctg ttc acc gca tac        192
Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr           
    50                  55                  60                            

aac ttt ctc atg tcc atc tac tcg ttc ggc tcc ttc ctg gcg atg gcc        240
Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala           
65                  70                  75                  80            

tac gct ctc tct gtc act ggt att ctt tcc ggc gat tgt gag act gcc        288
Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala           
                85                  90                  95                

ttc aac aat gac gtg ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc gtc gag tac atc gac tcc ttc tac ctt ccc ctc atg gac aag        384
Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys           
        115                 120                 125                       

ccc ttg tcg ttt ctg cag ttc ttt cac cat ctc gga gct ccc atc gac        432
Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aag tat cga aac gaa ggc gtc tgg atc ttt gtt        480
Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggt tac tat tgg acg        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctc atc aag ctg aac ttc cct atg ccc aag aac ctc att acc tcc        576
Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser           
            180                 185                 190                   

atg caa att atc cag ttc aac gtc gga ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cga aac gtg ccc tgc tac cgg cag gac ggt atg cga atg ttt gcc tgg        672
Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp           
    210                 215                 220                           

atc ttc aac tac tgg tat gtc ggc acg gtg ctg ctt ctg ttc ctc aac        720
Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtc cag acc tac att cgg aag cct cga aag aac cga ggc aaa        768
Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys           
                245                 250                 255               

aag gag                                                                774
Lys Glu                                                                   
                                                                          


<210>  14
<211>  258
<212>  PRT
<213>  Euglena anabaena UTEX 373

<400>  14

Met Glu Ala Ala Lys Glu Leu Val Ser Ile Val Gln Glu Glu Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Gln Asp Ala Ser Ser Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Val Ala Phe Val Ala Ile Lys Phe Met Leu Arg Pro 
        35                  40                  45              


Leu Asp Leu Lys Arg Gln Ala Thr Leu Lys Lys Leu Phe Thr Ala Tyr 
    50                  55                  60                  


Asn Phe Leu Met Ser Ile Tyr Ser Phe Gly Ser Phe Leu Ala Met Ala 
65                  70                  75                  80  


Tyr Ala Leu Ser Val Thr Gly Ile Leu Ser Gly Asp Cys Glu Thr Ala 
                85                  90                  95      


Phe Asn Asn Asp Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Val Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Asp Lys 
        115                 120                 125             


Pro Leu Ser Phe Leu Gln Phe Phe His His Leu Gly Ala Pro Ile Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Lys Tyr Arg Asn Glu Gly Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Asn Phe Pro Met Pro Lys Asn Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Val Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Ala Trp 
    210                 215                 220                 


Ile Phe Asn Tyr Trp Tyr Val Gly Thr Val Leu Leu Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Arg Lys Pro Arg Lys Asn Arg Gly Lys 
                245                 250                 255     


Lys Glu 
        


<210>  15
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = L or F

<220>
<221>  misc_feature
<222>  (5)..(8)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  US 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(13)

<400>  15

Tyr Asn Xaa Xaa Xaa Xaa Xaa Xaa Ser Xaa Xaa Ser Phe 
1               5                   10              


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X = E or D

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  X = T or S

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(15)

<400>  16

Phe Tyr Xaa Ser Lys Xaa Xaa Xaa Tyr Xaa Asp Xaa Xaa Xaa Leu 
1               5                   10                  15  


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  X = Q or H

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(9)

<400>  17

Leu Xaa Xaa Phe His His Xaa Gly Ala 
1               5                   


<210>  18
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(12)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  X = K or R or N

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(14)

<400>  18

Met Tyr Xaa Tyr Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe 
1               5                   10                  


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X = I or L or M

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(8)

<400>  19

Lys Xaa Leu Xaa Thr Xaa Xaa Gln 
1               5               


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(7)

<400>  20

Trp Xaa Phe Asn Tyr Xaa Tyr 
1               5           


<210>  21
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  delta-9 elongase motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<300>
<302>  DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY 
       ACIDS
<310>  U.S. 7,645,604
<311>  2006-11-16
<312>  2010-01-12
<313>  (1)..(9)

<400>  21

Tyr Xaa Gly Xaa Val Xaa Xaa Leu Phe 
1               5                   


<210>  22
<211>  258
<212>  PRT
<213>  Euglena gracilis


<220>
<221>  MISC_FEATURE
<222>  (1)..(258)
<223>  mutant delta-9 elongase; EgD9eS-mutant consensus

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa = Ser [S] (synthetic codon-optimized) or (Ala [A] or Asp [D] 
       or Gly [G] or Ile [I] or Lys [K] or Gln [Q]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa =  Gln [Q] (synthetic codon-optimized) or Lys [K] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa =  Ala [A] (synthetic codon-optimized) or (Asp [D] or Thr [T]
       or Val [V]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa =  Val [V] (synthetic codon-optimized) or Phe [F] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (35)..(35)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or (Phe [F] or Gly [G]
       or Met [M]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (58)..(58)
<223>  Xaa =  Lys [K] (synthetic codon-optimized) or Arg [R] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa =  Tyr [Y] (synthetic codon-optimized) or Cys [C] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (98)..(98)
<223>  Xaa =  Asp [D] (synthetic codon-optimized) or Gly [G] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (107)..(107)
<223>  Xaa =  Gln [Q] (synthetic codon-optimized) or Glu [E] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (108)..(108)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or Gly [G] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (127)..(127)
<223>  Xaa =  Gly [G] (synthetic codon-optimized) or Leu [L] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (130)..(130)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or Met [M] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (132)..(132)
<223>  Xaa =  Trp [W] (synthetic codon-optimized) or Thr [T] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (143)..(143)
<223>  Xaa =  Met [M] (synthetic codon-optimized) or (Asn [N] or Trp 
       [W]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (161)..(161)
<223>  Xaa =  Leu [L] (synthetic codon-optimized) or (Thr [T] or Tyr 
       [Y]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (168)..(168)
<223>  Xaa =  Trp [W] (synthetic codon-optimized) or Gly [G] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (179)..(179)
<223>  Xaa =  Ile [I] (synthetic codon-optimized) or (Met [M] or Arg 
       [R]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (236)..(236)
<223>  Xaa =  Cys [C] (synthetic codon-optimized) or Asn [N] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (243)..(243)
<223>  Xaa =  Val [V] (synthetic codon-optimized) or Ala [A] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (244)..(244)
<223>  Xaa =  Gln [Q] (synthetic codon-optimized) or Asn [N] (mutant)

<220>
<221>  MISC_FEATURE
<222>  (254)..(254)
<223>  Xaa =  Ala [A] (synthetic codon-optimized) or (Trp [W] or Tyr 
       [Y]) (mutant)

<220>
<221>  MISC_FEATURE
<222>  (257)..(257)
<223>  Xaa =  Ile [I] (synthetic codon-optimized) or Thr [T] (mutant)

<400>  22

Met Glu Val Val Asn Glu Ile Val Xaa Ile Gly Xaa Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Xaa Gln Leu Trp Ser Asp Ala Ser His Cys Glu Xaa 
            20                  25                  30          


Leu Tyr Xaa Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Xaa Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Xaa Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Xaa Asn Asn Val Phe Arg Ile Thr Thr Xaa Xaa Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Xaa Lys 
        115                 120                 125             


Pro Xaa Thr Xaa Leu Gln Phe Phe His His Leu Gly Ala Pro Xaa Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Xaa Leu Asn Gly Phe Ile His Xaa Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Xaa Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Xaa Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Xaa Xaa Thr Tyr Ile Val Arg Lys His Lys Gly Xaa Lys Lys 
                245                 250                 255     


Xaa Gln 
        


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant His box


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  23

Gln Xaa Xaa His His 
1               5   


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His box


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  24

His Xaa Xaa His His 
1               5   


<210>  25
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZUFmEgD9E

<400>  25
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZUFm_6980_012208f

<400>  26
gctctggtac catggaggtc                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pZUFm_40_012208r

<400>  27
acagaaccgg gcactcactt                                                   20


<210>  28
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35F"

<400>  28
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ttc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat ggg atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  29
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  29

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Phe Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  30
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZUFmEgD9eS-L35F

<400>  30
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acttctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tgggatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  31
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-K58R/I257T"

<400>  31
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg agg ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Arg Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tcg atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg caa ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

act cag tga                                                            777
Thr Gln                                                                   
                                                                          


<210>  32
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  32

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Arg Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Thr Gln 
        


<210>  33
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-K58R/I257T

<400>  33
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaggtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctcgatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc aattctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagac tcagtgagc                                       7769


<210>  34
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L130M/V243A1"

<400>  34
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct atg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aat tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gcg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  35
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  35

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  36
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-L130M/V243A1

<400>  36
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctatg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaat     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgcgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  37
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-D98G"

<400>  37
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc ggc aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Gly Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  38
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  38

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Gly Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  39
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-D98G

<400>  39
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cggcaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  40
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L130M/V243A2"

<400>  40
atg gag gtc gtg aac gaa att gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ctc tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct atg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aat tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gcg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  41
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  41

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Leu Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Met Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Ala Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  42
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9eS-L130M/V243A2

<400>  42
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat tgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acctctccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctatg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaat     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgcgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  43
<211>  289
<212>  PRT
<213>  Ciona intestinalis

<400>  43

Met Asp Val Leu His Arg Phe Leu Gly Phe Tyr Glu Trp Thr Leu Thr 
1               5                   10                  15      


Phe Ala Asp Pro Arg Val Ala Lys Trp Pro Leu Ile Glu Asn Pro Leu 
            20                  25                  30          


Pro Thr Ile Ala Ile Val Leu Leu Tyr Leu Ala Phe Val Leu Tyr Ile 
        35                  40                  45              


Gly Pro Arg Phe Met Arg Lys Arg Ala Pro Val Asp Phe Gly Leu Phe 
    50                  55                  60                  


Leu Pro Gly Tyr Asn Phe Ala Leu Val Ala Leu Asn Tyr Tyr Ile Leu 
65                  70                  75                  80  


Gln Glu Val Val Thr Gly Ser Tyr Gly Ala Gly Tyr Asp Leu Val Cys 
                85                  90                  95      


Thr Pro Leu Arg Ser Asp Ser Tyr Asp Pro Asn Glu Met Lys Val Ala 
            100                 105                 110         


Asn Ala Val Trp Trp Tyr Tyr Val Ser Lys Ile Ile Glu Leu Phe Asp 
        115                 120                 125             


Thr Val Leu Phe Thr Leu Arg Lys Arg Asp Arg Gln Val Thr Phe Leu 
    130                 135                 140                 


His Val Tyr His His Ser Thr Met Pro Leu Leu Trp Trp Ile Gly Ala 
145                 150                 155                 160 


Lys Trp Val Pro Gly Gly Gln Ser Phe Val Gly Ile Ile Leu Asn Ser 
                165                 170                 175     


Ser Val His Val Ile Met Tyr Thr Tyr Tyr Gly Leu Ser Ala Leu Gly 
            180                 185                 190         


Pro His Met Gln Lys Phe Leu Trp Trp Lys Lys Tyr Ile Thr Met Leu 
        195                 200                 205             


Gln Leu Val Gln Phe Val Leu Ala Ile Tyr His Thr Ala Arg Ser Leu 
    210                 215                 220                 


Tyr Val Lys Cys Pro Ser Pro Val Trp Met His Trp Ala Leu Ile Leu 
225                 230                 235                 240 


Tyr Ala Phe Ser Phe Ile Leu Leu Phe Ser Asn Phe Tyr Met His Ala 
                245                 250                 255     


Tyr Ile Lys Lys Ser Arg Lys Gly Lys Glu Asn Gly Ser Arg Gly Lys 
            260                 265                 270         


Gly Gly Val Ser Asn Gly Lys Glu Lys Leu His Ala Asn Gly Lys Thr 
        275                 280                 285             


Asp 
    


<210>  44
<211>  295
<212>  PRT
<213>  Oncorhynchus mykiss

<400>  44

Met Glu Thr Phe Asn Tyr Lys Leu Asn Met Tyr Ile Asp Ser Trp Met 
1               5                   10                  15      


Gly Pro Arg Asp Glu Arg Val Gln Gly Trp Leu Leu Leu Asp Asn Tyr 
            20                  25                  30          


Pro Pro Thr Phe Ala Leu Thr Val Met Tyr Leu Leu Ile Val Trp Met 
        35                  40                  45              


Gly Pro Lys Tyr Met Arg His Arg Gln Pro Val Ser Cys Arg Gly Leu 
    50                  55                  60                  


Leu Leu Val Tyr Asn Leu Gly Leu Thr Ile Leu Ser Phe Tyr Met Phe 
65                  70                  75                  80  


Tyr Glu Met Val Ser Ala Val Trp His Gly Asp Tyr Asn Phe Phe Cys 
                85                  90                  95      


Gln Asp Thr His Ser Ala Gly Glu Thr Asp Thr Lys Ile Ile Asn Val 
            100                 105                 110         


Leu Trp Trp Tyr Tyr Phe Ser Lys Leu Ile Glu Phe Met Asp Thr Phe 
        115                 120                 125             


Phe Phe Ile Leu Arg Lys Asn Asn His Gln Ile Thr Phe Leu His Ile 
    130                 135                 140                 


Tyr His His Ala Ser Met Leu Asn Ile Trp Trp Phe Val Met Asn Trp 
145                 150                 155                 160 


Val Pro Cys Gly His Ser Tyr Phe Gly Ala Ser Leu Asn Ser Phe Ile 
                165                 170                 175     


His Val Leu Met Tyr Ser Tyr Tyr Gly Leu Ser Ala Val Pro Ala Leu 
            180                 185                 190         


Arg Pro Tyr Leu Trp Trp Lys Lys Tyr Ile Thr Gln Val Gln Leu Ile 
        195                 200                 205             


Gln Phe Phe Leu Thr Met Ser Gln Thr Ile Cys Ala Val Ile Trp Pro 
    210                 215                 220                 


Cys Asp Phe Pro Arg Gly Trp Leu Tyr Phe Gln Ile Phe Tyr Val Ile 
225                 230                 235                 240 


Thr Leu Ile Ala Leu Phe Ser Asn Phe Tyr Ile Gln Thr Tyr Lys Lys 
                245                 250                 255     


His Leu Val Ser Gln Lys Lys Glu Tyr His Gln Asn Gly Ser Val Ala 
            260                 265                 270         


Ser Leu Asn Gly His Val Asn Gly Val Thr Pro Thr Glu Thr Ile Thr 
        275                 280                 285             


His Arg Lys Val Arg Gly Asp 
    290                 295 


<210>  45
<211>  290
<212>  PRT
<213>  Marchantia polymorpha

<400>  45

Met Glu Ala Tyr Glu Met Val Asp Ser Phe Val Ser Lys Thr Val Phe 
1               5                   10                  15      


Glu Thr Leu Gln Arg Leu Arg Gly Gly Val Val Leu Thr Glu Ser Ala 
            20                  25                  30          


Ile Thr Lys Gly Leu Pro Cys Val Asp Ser Pro Thr Pro Ile Val Leu 
        35                  40                  45              


Gly Leu Ser Ser Tyr Leu Thr Phe Val Phe Leu Gly Leu Ile Val Ile 
    50                  55                  60                  


Lys Ser Leu Asp Leu Lys Pro Arg Ser Lys Glu Pro Ala Ile Leu Asn 
65                  70                  75                  80  


Leu Phe Val Ile Phe His Asn Phe Val Cys Phe Ala Leu Ser Leu Tyr 
                85                  90                  95      


Met Cys Val Gly Ile Val Arg Gln Ala Ile Leu Asn Arg Tyr Ser Leu 
            100                 105                 110         


Trp Gly Asn Ala Tyr Asn Pro Lys Glu Val Gln Met Gly His Leu Leu 
        115                 120                 125             


Tyr Ile Phe Tyr Met Ser Lys Tyr Ile Glu Phe Met Asp Thr Val Ile 
    130                 135                 140                 


Met Ile Leu Lys Arg Asn Thr Arg Gln Ile Thr Val Leu His Val Tyr 
145                 150                 155                 160 


His His Ala Ser Ile Ser Phe Ile Trp Trp Ile Ile Ala Tyr His Ala 
                165                 170                 175     


Pro Gly Gly Glu Ala Tyr Phe Ser Ala Ala Leu Asn Ser Gly Val His 
            180                 185                 190         


Val Leu Met Tyr Leu Tyr Tyr Leu Leu Ala Ala Thr Leu Gly Lys Asn 
        195                 200                 205             


Glu Lys Ala Arg Arg Lys Tyr Leu Trp Trp Gly Lys Tyr Leu Thr Gln 
    210                 215                 220                 


Leu Gln Met Phe Gln Phe Val Leu Asn Met Ile Gln Ala Tyr Tyr Asp 
225                 230                 235                 240 


Ile Lys Asn Asn Ser Pro Tyr Pro Gln Phe Leu Ile Gln Ile Leu Phe 
                245                 250                 255     


Tyr Tyr Met Ile Ser Leu Leu Ala Leu Phe Gly Asn Phe Tyr Val His 
            260                 265                 270         


Lys Tyr Val Ser Ala Pro Ala Lys Pro Ala Lys Ile Lys Ser Lys Lys 
        275                 280                 285             


Ala Glu 
    290 


<210>  46
<211>  290
<212>  PRT
<213>  Physcomitrella patens

<400>  46

Met Glu Val Val Glu Arg Phe Tyr Gly Glu Leu Asp Gly Lys Val Ser 
1               5                   10                  15      


Gln Gly Val Asn Ala Leu Leu Gly Ser Phe Gly Val Glu Leu Thr Asp 
            20                  25                  30          


Thr Pro Thr Thr Lys Gly Leu Pro Leu Val Asp Ser Pro Thr Pro Ile 
        35                  40                  45              


Val Leu Gly Val Ser Val Tyr Leu Thr Ile Val Ile Gly Gly Leu Leu 
    50                  55                  60                  


Trp Ile Lys Ala Arg Asp Leu Lys Pro Arg Ala Ser Glu Pro Phe Leu 
65                  70                  75                  80  


Leu Gln Ala Leu Val Leu Val His Asn Leu Phe Cys Phe Ala Leu Ser 
                85                  90                  95      


Leu Tyr Met Cys Val Gly Ile Ala Tyr Gln Ala Ile Thr Trp Arg Tyr 
            100                 105                 110         


Ser Leu Trp Gly Asn Ala Tyr Asn Pro Lys His Lys Glu Met Ala Ile 
        115                 120                 125             


Leu Val Tyr Leu Phe Tyr Met Ser Lys Tyr Val Glu Phe Met Asp Thr 
    130                 135                 140                 


Val Ile Met Ile Leu Lys Arg Ser Thr Arg Gln Ile Ser Phe Leu His 
145                 150                 155                 160 


Val Tyr His His Ser Ser Ile Ser Leu Ile Trp Trp Ala Ile Ala His 
                165                 170                 175     


His Ala Pro Gly Gly Glu Ala Tyr Trp Ser Ala Ala Leu Asn Ser Gly 
            180                 185                 190         


Val His Val Leu Met Tyr Ala Tyr Tyr Phe Leu Ala Ala Cys Leu Arg 
        195                 200                 205             


Ser Ser Pro Lys Leu Lys Asn Lys Tyr Leu Phe Trp Gly Arg Tyr Leu 
    210                 215                 220                 


Thr Gln Phe Gln Met Phe Gln Phe Met Leu Asn Leu Val Gln Ala Tyr 
225                 230                 235                 240 


Tyr Asp Met Lys Thr Asn Ala Pro Tyr Pro Gln Trp Leu Ile Lys Ile 
                245                 250                 255     


Leu Phe Tyr Tyr Met Ile Ser Leu Leu Phe Leu Phe Gly Asn Phe Tyr 
            260                 265                 270         


Val Gln Lys Tyr Ile Lys Pro Ser Asp Gly Lys Gln Lys Gly Ala Lys 
        275                 280                 285             


Thr Glu 
    290 


<210>  47
<211>  348
<212>  PRT
<213>  Marchantia polymorpha

<400>  47

Met Ala Thr Lys Ser Gly Ser Gly Leu Leu Glu Trp Ile Ala Val Ala 
1               5                   10                  15      


Ala Lys Met Lys Gln Ala Arg Ser Ser Pro Glu Gly Glu Ile Val Gly 
            20                  25                  30          


Gly Asn Arg Met Gly Ser Gly Asn Gly Ala Glu Trp Thr Thr Ser Leu 
        35                  40                  45              


Ile His Ala Phe Leu Asn Ala Thr Asn Gly Lys Ser Gly Gly Ala Ser 
    50                  55                  60                  


Lys Val Arg Pro Leu Glu Glu Arg Ile Gly Glu Ala Val Phe Arg Val 
65                  70                  75                  80  


Leu Glu Asp Val Val Gly Val Asp Ile Arg Lys Pro Asn Pro Val Thr 
                85                  90                  95      


Lys Asp Leu Pro Met Val Glu Ser Pro Val Pro Val Leu Ala Cys Ile 
            100                 105                 110         


Ser Leu Tyr Leu Leu Val Val Trp Leu Trp Ser Ser His Ile Lys Ala 
        115                 120                 125             


Ser Gly Gln Lys Pro Arg Lys Glu Asp Pro Leu Ala Leu Arg Cys Leu 
    130                 135                 140                 


Val Ile Ala His Asn Leu Phe Leu Cys Cys Leu Ser Leu Phe Met Cys 
145                 150                 155                 160 


Val Gly Leu Ile Ala Ala Ala Arg His Tyr Gly Tyr Ser Val Trp Gly 
                165                 170                 175     


Asn Tyr Tyr Arg Glu Arg Glu Pro Ala Met Asn Leu Leu Ile Tyr Val 
            180                 185                 190         


Phe Tyr Met Ser Lys Leu Tyr Glu Phe Met Asp Thr Ala Ile Met Leu 
        195                 200                 205             


Phe Arg Arg Asn Leu Arg Gln Val Thr Tyr Leu His Val Tyr His His 
    210                 215                 220                 


Ala Ser Ile Ala Met Ile Trp Trp Ile Ile Cys Tyr Arg Phe Pro Gly 
225                 230                 235                 240 


Ala Asp Ser Tyr Phe Ser Ala Ala Phe Asn Ser Cys Ile His Val Ala 
                245                 250                 255     


Met Tyr Leu Tyr Tyr Leu Leu Ala Ala Thr Val Ala Arg Asp Glu Lys 
            260                 265                 270         


Arg Arg Arg Lys Tyr Leu Phe Trp Gly Lys Tyr Leu Thr Ile Ile Gln 
        275                 280                 285             


Met Leu Gln Phe Leu Ser Phe Ile Gly Gln Ala Ile Tyr Ala Met Trp 
    290                 295                 300                 


Lys Phe Glu Tyr Tyr Pro Lys Gly Phe Gly Arg Met Leu Phe Phe Tyr 
305                 310                 315                 320 


Ser Val Ser Leu Leu Ala Phe Phe Gly Asn Phe Phe Val Lys Lys Tyr 
                325                 330                 335     


Ser Asn Ala Ser Gln Pro Lys Thr Val Lys Val Glu 
            340                 345             


<210>  48
<211>  292
<212>  PRT
<213>  Ostreococcus tauri

<400>  48

Met Ser Gly Leu Arg Ala Pro Asn Phe Leu His Arg Phe Trp Thr Lys 
1               5                   10                  15      


Trp Asp Tyr Ala Ile Ser Lys Val Val Phe Thr Cys Ala Asp Ser Phe 
            20                  25                  30          


Gln Trp Asp Ile Gly Pro Val Ser Ser Ser Thr Ala His Leu Pro Ala 
        35                  40                  45              


Ile Glu Ser Pro Thr Pro Leu Val Thr Ser Leu Leu Phe Tyr Leu Val 
    50                  55                  60                  


Thr Val Phe Leu Trp Tyr Gly Arg Leu Thr Arg Ser Ser Asp Lys Lys 
65                  70                  75                  80  


Ile Arg Glu Pro Thr Trp Leu Arg Arg Phe Ile Ile Cys His Asn Ala 
                85                  90                  95      


Phe Leu Ile Val Leu Ser Leu Tyr Met Cys Leu Gly Cys Val Ala Gln 
            100                 105                 110         


Ala Tyr Gln Asn Gly Tyr Thr Leu Trp Gly Asn Glu Phe Lys Ala Thr 
        115                 120                 125             


Glu Thr Gln Leu Ala Leu Tyr Ile Tyr Ile Phe Tyr Val Ser Lys Ile 
    130                 135                 140                 


Tyr Glu Phe Val Asp Thr Tyr Ile Met Leu Leu Lys Asn Asn Leu Arg 
145                 150                 155                 160 


Gln Val Ser Phe Leu His Ile Tyr His His Ser Thr Ile Ser Phe Ile 
                165                 170                 175     


Trp Trp Ile Ile Ala Arg Arg Ala Pro Gly Gly Asp Ala Tyr Phe Ser 
            180                 185                 190         


Ala Ala Leu Asn Ser Trp Val His Val Cys Met Tyr Thr Tyr Tyr Leu 
        195                 200                 205             


Leu Ser Thr Leu Ile Gly Lys Glu Asp Pro Lys Arg Ser Asn Tyr Leu 
    210                 215                 220                 


Trp Trp Gly Arg His Leu Thr Gln Met Gln Met Leu Gln Phe Phe Phe 
225                 230                 235                 240 


Asn Val Leu Gln Ala Leu Tyr Cys Ala Ser Phe Ser Thr Tyr Pro Lys 
                245                 250                 255     


Phe Leu Ser Lys Ile Leu Leu Val Tyr Met Met Ser Leu Leu Gly Leu 
            260                 265                 270         


Phe Gly His Phe Tyr Tyr Ser Lys His Ile Ala Ala Ala Lys Leu Gln 
        275                 280                 285             


Lys Lys Gln Gln 
    290         


<210>  49
<211>  277
<212>  PRT
<213>  Pavlova sp.

<400>  49

Met Met Leu Ala Ala Gly Tyr Leu Leu Val Leu Ser Ala Ala Arg Gln 
1               5                   10                  15      


Ser Phe Gln Gln Asp Ile Asp Asn Pro Asn Gly Ala Tyr Ser Thr Ser 
            20                  25                  30          


Trp Thr Gly Leu Pro Ile Val Met Ser Val Val Tyr Leu Ser Gly Val 
        35                  40                  45              


Phe Gly Leu Thr Lys Tyr Phe Glu Asn Arg Lys Pro Met Thr Gly Leu 
    50                  55                  60                  


Lys Asp Tyr Met Phe Thr Tyr Asn Leu Tyr Gln Val Ile Ile Asn Val 
65                  70                  75                  80  


Trp Cys Val Val Ala Phe Leu Leu Glu Val Arg Arg Ala Gly Met Ser 
                85                  90                  95      


Leu Ile Gly Asn Lys Val Asp Leu Gly Pro Asn Ser Phe Arg Leu Gly 
            100                 105                 110         


Phe Val Thr Trp Val His Tyr Asn Asn Lys Tyr Val Glu Leu Leu Asp 
        115                 120                 125             


Thr Leu Trp Met Val Leu Arg Lys Lys Thr Gln Gln Val Ser Phe Leu 
    130                 135                 140                 


His Val Tyr His His Val Leu Leu Met Trp Ala Trp Phe Val Val Val 
145                 150                 155                 160 


Lys Leu Gly Asn Gly Gly Asp Ala Tyr Phe Gly Gly Leu Met Asn Ser 
                165                 170                 175     


Ile Ile His Val Met Met Tyr Ser Tyr Tyr Thr Met Ala Leu Leu Gly 
            180                 185                 190         


Trp Ser Cys Pro Trp Lys Arg Tyr Leu Thr Gln Ala Gln Leu Val Gln 
        195                 200                 205             


Phe Cys Ile Cys Leu Ala His Ser Thr Trp Ala Ala Val Thr Gly Ala 
    210                 215                 220                 


Tyr Pro Trp Arg Ile Cys Leu Val Glu Val Trp Val Met Val Ser Met 
225                 230                 235                 240 


Leu Val Leu Phe Thr Arg Phe Tyr Arg Gln Ala Tyr Ala Lys Glu Ala 
                245                 250                 255     


Lys Ala Lys Glu Ala Lys Lys Leu Ala Gln Glu Ala Ser Gln Ala Lys 
            260                 265                 270         


Ala Val Lys Ala Glu 
        275         


<210>  50
<211>  302
<212>  PRT
<213>  Rebecca salina

<400>  50

Met Lys Ala Ala Ala Gly Lys Val Gln Gln Glu Ala Glu Arg Leu Thr 
1               5                   10                  15      


Ala Gly Leu Trp Leu Pro Met Met Leu Ala Ala Gly Tyr Leu Leu Val 
            20                  25                  30          


Leu Ser Ala Asn Arg Ala Ser Phe Tyr Glu Asn Ile Asn Asn Glu Lys 
        35                  40                  45              


Gly Ala Tyr Ser Thr Ser Trp Phe Ser Leu Pro Cys Val Met Thr Ala 
    50                  55                  60                  


Val Tyr Leu Gly Gly Val Phe Gly Leu Thr Lys Tyr Phe Glu Gly Arg 
65                  70                  75                  80  


Lys Pro Met Gln Gly Leu Lys Asp Tyr Met Phe Thr Tyr Asn Leu Tyr 
                85                  90                  95      


Gln Val Ile Ile Asn Val Trp Cys Ile Ala Ala Phe Val Val Glu Val 
            100                 105                 110         


Arg Arg Ala Gly Met Ser Ala Val Gly Asn Lys Val Asp Leu Gly Pro 
        115                 120                 125             


Asn Ser Phe Arg Leu Gly Phe Val Thr Trp Val His Tyr Asn Asn Lys 
    130                 135                 140                 


Tyr Val Glu Leu Leu Asp Thr Leu Trp Met Val Leu Arg Lys Lys Thr 
145                 150                 155                 160 


Gln Gln Val Ser Phe Leu His Val Tyr His His Val Leu Leu Ile Trp 
                165                 170                 175     


Ala Trp Phe Cys Val Val Lys Phe Cys Asn Gly Gly Asp Ala Tyr Phe 
            180                 185                 190         


Gly Gly Met Leu Asn Ser Ile Ile His Val Met Met Tyr Ser Tyr Tyr 
        195                 200                 205             


Thr Met Ala Leu Leu Gly Trp Ser Cys Pro Trp Lys Arg Tyr Leu Thr 
    210                 215                 220                 


Gln Ala Gln Leu Val Gln Phe Cys Ile Cys Leu Ala His Ala Thr Trp 
225                 230                 235                 240 


Ala Ala Ala Thr Gly Val Tyr Pro Phe His Ile Cys Leu Val Glu Ile 
                245                 250                 255     


Trp Val Met Val Ser Met Leu Tyr Leu Phe Thr Lys Phe Tyr Asn Ser 
            260                 265                 270         


Ala Tyr Lys Gly Ala Ala Lys Gly Ala Ala Ala Ser Ser Asn Gly Ala 
        275                 280                 285             


Ala Ala Pro Ser Gly Ala Lys Pro Lys Ser Ile Lys Ala Asn 
    290                 295                 300         


<210>  51
<211>  300
<212>  PRT
<213>  Ostreococcus tauri

<400>  51

Met Ser Ala Ser Gly Ala Leu Leu Pro Ala Ile Ala Ser Ala Ala Tyr 
1               5                   10                  15      


Ala Tyr Ala Thr Tyr Ala Tyr Ala Phe Glu Trp Ser His Ala Asn Gly 
            20                  25                  30          


Ile Asp Asn Val Asp Ala Arg Glu Trp Ile Gly Ala Leu Ser Leu Arg 
        35                  40                  45              


Leu Pro Ala Ile Ala Thr Thr Met Tyr Leu Leu Phe Cys Leu Val Gly 
    50                  55                  60                  


Pro Arg Leu Met Ala Lys Arg Glu Ala Phe Asp Pro Lys Gly Phe Met 
65                  70                  75                  80  


Leu Ala Tyr Asn Ala Tyr Gln Thr Ala Phe Asn Val Val Val Leu Gly 
                85                  90                  95      


Met Phe Ala Arg Glu Ile Ser Gly Leu Gly Gln Pro Val Trp Gly Ser 
            100                 105                 110         


Thr Met Pro Trp Ser Asp Arg Lys Ser Phe Lys Ile Leu Leu Gly Val 
        115                 120                 125             


Trp Leu His Tyr Asn Asn Lys Tyr Leu Glu Leu Leu Asp Thr Val Phe 
    130                 135                 140                 


Met Val Ala Arg Lys Lys Thr Lys Gln Leu Ser Phe Leu His Val Tyr 
145                 150                 155                 160 


His His Ala Leu Leu Ile Trp Ala Trp Trp Leu Val Cys His Leu Met 
                165                 170                 175     


Ala Thr Asn Asp Cys Ile Asp Ala Tyr Phe Gly Ala Ala Cys Asn Ser 
            180                 185                 190         


Phe Ile His Ile Val Met Tyr Ser Tyr Tyr Leu Met Ser Ala Leu Gly 
        195                 200                 205             


Ile Arg Cys Pro Trp Lys Arg Tyr Ile Thr Gln Ala Gln Met Leu Gln 
    210                 215                 220                 


Phe Val Ile Val Phe Ala His Ala Val Phe Val Leu Arg Gln Lys His 
225                 230                 235                 240 


Cys Pro Val Thr Leu Pro Trp Ala Gln Met Phe Val Met Thr Asn Met 
                245                 250                 255     


Leu Val Leu Phe Gly Asn Phe Tyr Leu Lys Ala Tyr Ser Asn Lys Ser 
            260                 265                 270         


Arg Gly Asp Gly Ala Ser Ser Val Lys Pro Ala Glu Thr Thr Arg Ala 
        275                 280                 285             


Pro Ser Val Arg Arg Thr Arg Ser Arg Lys Ile Asp 
    290                 295                 300 


<210>  52
<211>  358
<212>  PRT
<213>  Thalassiosira pseudonana

<400>  52

Met Cys Ser Ser Pro Pro Ser Gln Ser Lys Thr Thr Ser Leu Leu Ala 
1               5                   10                  15      


Arg Tyr Thr Thr Ala Ala Leu Leu Leu Leu Thr Leu Thr Thr Trp Cys 
            20                  25                  30          


His Phe Ala Phe Pro Ala Ala Thr Ala Thr Pro Gly Leu Thr Ala Glu 
        35                  40                  45              


Met His Ser Tyr Lys Val Pro Leu Gly Leu Thr Val Phe Tyr Leu Leu 
    50                  55                  60                  


Ser Leu Pro Ser Leu Lys Tyr Val Thr Asp Asn Tyr Leu Ala Lys Lys 
65                  70                  75                  80  


Tyr Asp Met Lys Ser Leu Leu Thr Glu Ser Met Val Leu Tyr Asn Val 
                85                  90                  95      


Ala Gln Val Leu Leu Asn Gly Trp Thr Val Tyr Ala Ile Val Asp Ala 
            100                 105                 110         


Val Met Asn Arg Asp His Pro Phe Ile Gly Ser Arg Ser Leu Val Gly 
        115                 120                 125             


Ala Ala Leu His Ser Gly Ser Ser Tyr Ala Val Trp Val His Tyr Cys 
    130                 135                 140                 


Asp Lys Tyr Leu Glu Phe Phe Asp Thr Tyr Phe Met Val Leu Arg Gly 
145                 150                 155                 160 


Lys Met Asp Gln Val Ser Phe Leu His Ile Tyr His His Thr Thr Ile 
                165                 170                 175     


Ala Trp Ala Trp Trp Ile Ala Leu Arg Phe Ser Pro Gly Gly Asp Ile 
            180                 185                 190         


Tyr Phe Gly Ala Leu Leu Asn Ser Ile Ile His Val Leu Met Tyr Ser 
        195                 200                 205             


Tyr Tyr Ala Leu Ala Leu Leu Lys Val Ser Cys Pro Trp Lys Arg Tyr 
    210                 215                 220                 


Leu Thr Gln Ala Gln Leu Leu Gln Phe Thr Ser Val Val Val Tyr Thr 
225                 230                 235                 240 


Gly Cys Thr Gly Tyr Thr His Tyr Tyr His Thr Lys His Gly Ala Asp 
                245                 250                 255     


Glu Thr Gln Pro Ser Leu Gly Thr Tyr Tyr Phe Cys Cys Gly Val Gln 
            260                 265                 270         


Val Phe Glu Met Val Ser Leu Phe Val Leu Phe Ser Ile Phe Tyr Lys 
        275                 280                 285             


Arg Ser Tyr Ser Lys Lys Asn Lys Ser Gly Gly Lys Asp Ser Lys Lys 
    290                 295                 300                 


Asn Asp Asp Gly Asn Asn Glu Asp Gln Cys His Lys Ala Met Lys Asp 
305                 310                 315                 320 


Ile Ser Glu Gly Ala Lys Glu Val Val Gly His Ala Ala Lys Asp Ala 
                325                 330                 335     


Gly Lys Leu Val Ala Thr Ala Ser Lys Ala Val Lys Arg Lys Gly Thr 
            340                 345                 350         


Arg Val Thr Gly Ala Met 
        355             


<210>  53
<211>  272
<212>  PRT
<213>  Thalassiosira pseudonana

<400>  53

Met Asp Ala Tyr Asn Ala Ala Met Asp Lys Ile Gly Ala Ala Ile Ile 
1               5                   10                  15      


Asp Trp Ser Asp Pro Asp Gly Lys Phe Arg Ala Asp Arg Glu Asp Trp 
            20                  25                  30          


Trp Leu Cys Asp Phe Arg Ser Ala Ile Thr Ile Ala Leu Ile Tyr Ile 
        35                  40                  45              


Ala Phe Val Ile Leu Gly Ser Ala Val Met Gln Ser Leu Pro Ala Met 
    50                  55                  60                  


Asp Pro Tyr Pro Ile Lys Phe Leu Tyr Asn Val Ser Gln Ile Phe Leu 
65                  70                  75                  80  


Cys Ala Tyr Met Thr Val Glu Ala Gly Phe Leu Ala Tyr Arg Asn Gly 
                85                  90                  95      


Tyr Thr Val Met Pro Cys Asn His Phe Asn Val Asn Asp Pro Pro Val 
            100                 105                 110         


Ala Asn Leu Leu Trp Leu Phe Tyr Ile Ser Lys Val Trp Asp Phe Trp 
        115                 120                 125             


Asp Thr Ile Phe Ile Val Leu Gly Lys Lys Trp Arg Gln Leu Ser Phe 
    130                 135                 140                 


Leu His Val Tyr His His Thr Thr Ile Phe Leu Phe Tyr Trp Leu Asn 
145                 150                 155                 160 


Ala Asn Val Leu Tyr Asp Gly Asp Ile Phe Leu Thr Ile Leu Leu Asn 
                165                 170                 175     


Gly Phe Ile His Thr Val Met Tyr Thr Tyr Tyr Phe Ile Cys Met His 
            180                 185                 190         


Thr Lys Asp Ser Lys Thr Gly Lys Ser Leu Pro Ile Trp Trp Lys Ser 
        195                 200                 205             


Ser Leu Thr Ala Phe Gln Leu Leu Gln Phe Thr Ile Met Met Ser Gln 
    210                 215                 220                 


Ala Thr Tyr Leu Val Phe His Gly Cys Asp Lys Val Ser Leu Arg Ile 
225                 230                 235                 240 


Thr Ile Val Tyr Phe Val Ser Leu Leu Ser Leu Phe Phe Leu Phe Ala 
                245                 250                 255     


Gln Phe Phe Val Gln Ser Tyr Met Ala Pro Lys Lys Lys Lys Ser Ala 
            260                 265                 270         


<210>  54
<211>  318
<212>  PRT
<213>  Mortierella alpina

<400>  54

Met Glu Ser Ile Ala Pro Phe Leu Pro Ser Lys Met Pro Gln Asp Leu 
1               5                   10                  15      


Phe Met Asp Leu Ala Thr Ala Ile Gly Val Arg Ala Ala Pro Tyr Val 
            20                  25                  30          


Asp Pro Leu Glu Ala Ala Leu Val Ala Gln Ala Glu Lys Tyr Ile Pro 
        35                  40                  45              


Thr Ile Val His His Thr Arg Gly Phe Leu Val Ala Val Glu Ser Pro 
    50                  55                  60                  


Leu Ala Arg Glu Leu Pro Leu Met Asn Pro Phe His Val Leu Leu Ile 
65                  70                  75                  80  


Val Leu Ala Tyr Leu Val Thr Val Phe Val Gly Met Gln Ile Met Lys 
                85                  90                  95      


Asn Phe Glu Arg Phe Glu Val Lys Thr Phe Ser Leu Leu His Asn Phe 
            100                 105                 110         


Cys Leu Val Ser Ile Ser Ala Tyr Met Cys Gly Gly Ile Leu Tyr Glu 
        115                 120                 125             


Ala Tyr Gln Ala Asn Tyr Gly Leu Phe Glu Asn Ala Ala Asp His Thr 
    130                 135                 140                 


Phe Lys Gly Leu Pro Met Ala Lys Met Ile Trp Leu Phe Tyr Phe Ser 
145                 150                 155                 160 


Lys Ile Met Glu Phe Val Asp Thr Met Ile Met Val Leu Lys Lys Asn 
                165                 170                 175     


Asn Arg Gln Ile Ser Phe Leu His Val Tyr His His Ser Ser Ile Phe 
            180                 185                 190         


Thr Ile Trp Trp Leu Val Thr Phe Val Ala Pro Asn Gly Glu Ala Tyr 
        195                 200                 205             


Phe Ser Ala Ala Leu Asn Ser Phe Ile His Val Ile Met Tyr Gly Tyr 
    210                 215                 220                 


Tyr Phe Leu Ser Ala Leu Gly Phe Lys Gln Val Ser Phe Ile Lys Phe 
225                 230                 235                 240 


Tyr Ile Thr Arg Ser Gln Met Thr Gln Phe Cys Met Met Ser Val Gln 
                245                 250                 255     


Ser Ser Trp Asp Met Tyr Ala Met Lys Val Leu Gly Arg Pro Gly Tyr 
            260                 265                 270         


Pro Phe Phe Ile Thr Ala Leu Leu Trp Phe Tyr Met Trp Thr Met Leu 
        275                 280                 285             


Gly Leu Phe Tyr Asn Phe Tyr Arg Lys Asn Ala Lys Leu Ala Lys Gln 
    290                 295                 300                 


Ala Lys Ala Asp Ala Ala Lys Glu Lys Ala Arg Lys Leu Gln 
305                 310                 315             


<210>  55
<211>  271
<212>  PRT
<213>  Thraustochytrium sp.

<400>  55

Met Met Glu Pro Leu Asp Arg Tyr Arg Ala Leu Ala Glu Leu Ala Ala 
1               5                   10                  15      


Arg Tyr Ala Ser Ser Ala Ala Phe Lys Trp Gln Val Thr Tyr Asp Ala 
            20                  25                  30          


Lys Asp Ser Phe Val Gly Pro Leu Gly Ile Arg Glu Pro Leu Gly Leu 
        35                  40                  45              


Leu Val Gly Ser Val Val Leu Tyr Leu Ser Leu Gln Ala Val Val Tyr 
    50                  55                  60                  


Ala Leu Arg Asn Tyr Leu Gly Gly Leu Met Ala Leu Arg Ser Val His 
65                  70                  75                  80  


Asn Leu Gly Leu Cys Leu Phe Ser Gly Ala Val Trp Ile Tyr Thr Ser 
                85                  90                  95      


Tyr Leu Met Ile Gln Asp Gly His Phe Arg Ser Leu Glu Ala Ala Thr 
            100                 105                 110         


Cys Glu Pro Leu Lys His Pro His Phe Gln Leu Ile Ser Leu Leu Phe 
        115                 120                 125             


Ala Leu Ser Lys Ile Trp Glu Trp Phe Asp Thr Val Leu Leu Ile Val 
    130                 135                 140                 


Lys Gly Asn Lys Leu Arg Phe Leu His Val Leu His His Ala Thr Thr 
145                 150                 155                 160 


Phe Trp Leu Tyr Ala Ile Asp His Ile Phe Leu Ser Ser Ile Lys Tyr 
                165                 170                 175     


Gly Val Ala Val Asn Ala Phe Ile His Thr Val Met Tyr Ala His Tyr 
            180                 185                 190         


Phe Arg Pro Phe Pro Lys Gly Leu Arg Pro Leu Ile Thr Gln Leu Gln 
        195                 200                 205             


Ile Val Gln Phe Ile Phe Ser Ile Gly Ile His Thr Ala Ile Tyr Trp 
    210                 215                 220                 


His Tyr Asp Cys Glu Pro Leu Val His Thr His Phe Trp Glu Tyr Val 
225                 230                 235                 240 


Thr Pro Tyr Leu Phe Val Val Pro Phe Leu Ile Leu Phe Leu Asn Phe 
                245                 250                 255     


Tyr Leu Gln Gln Tyr Val Leu Ala Pro Ala Lys Thr Lys Lys Ala 
            260                 265                 270     


<210>  56
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EgD9E_102_053008f


<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  n is a, c, g, or t

<400>  56
gtgctgtacn nktccatcgc ctt                                               23


<210>  57
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer EgD9E_760_053008r

<400>  57
tcccttgtgc tttcggacga tgta                                              24


<210>  58
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  58
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  59
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  59

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  60
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9ES-L35G

<400>  60
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  61
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35M/Q107E"

<400>  61
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac atg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Met Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act gag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Glu Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg atc aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  62
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  62

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Met Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Glu Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Ile Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  63
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9ES-L35M/Q107E

<400>  63
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acatgtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactga gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgatcaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  64
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_9D_122709f

<400>  64
gaaatcgtcg acattggcca gg                                                22


<210>  65
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_84C_122709r

<400>  65
gacaccaatg gtacacatgg cgtaggc                                           27


<210>  66
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_84C_122709f

<400>  66
gcctacgcca tgtgtaccat tggtgtc                                           27


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_9D_122709r

<400>  67
cctggccaat gtcgacgatt tc                                                22


<210>  68
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_161T_122709f

<400>  68
gatctttgtg accctcaacg gcttc                                             25


<210>  69
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_179R_122709r

<400>  69
gaacttgagc tttcgcagtc gggtc                                             25


<210>  70
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_179R_122709f

<400>  70
gacccgactg cgaaagctca agttc                                             25


<210>  71
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_161T_122709r

<400>  71
gaagccgttg agggtcacaa agatc                                             25


<210>  72
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_244N_122709f

<400>  72
cttctacgtg aacacctaca tc                                                22


<210>  73
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_21V_010710r

<400>  73
gaccagagct gcacatagtc gac                                               23


<210>  74
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_21V_010710f

<400>  74
gtcgactatg tgcagctctg gtc                                               23


<210>  75
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_244N_122709r

<400>  75
gatgtaggtg ttcacgtaga ag                                                22


<210>  76
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_32F_010710f

<400>  76
gcactgcgag tttctgtacc tc                                                22


<210>  77
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_108G_010710r

<400>  77
gaggtagaaa ccctgagtgg tg                                                22


<210>  78
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_108G_010710f

<400>  78
caccactcag ggtttctacc tc                                                22


<210>  79
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_32F_010710r

<400>  79
gaggtacaga aactcgcagt gc                                                22


<210>  80
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_132T_010710f

<400>  80
gcctctgacc accttgcagt tc                                                22


<210>  81
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_143N_010710r

<400>  81
gccacatgtc gttaggagct cc                                                22


<210>  82
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_143N_010710f

<400>  82
ggagctccta acgacatgtg gc                                                22


<210>  83
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_132T_010710r

<400>  83
gaactgcaag gtggtcagag gc                                                22


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_236N_010710f

<400>  84
gtactgtcct gaacctgttc ctc                                               23


<210>  85
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Eg_236N_010710r

<400>  85
gaggaacagg ttcaggacag tac                                               23


<210>  86
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-A21V/L35G/L108G/I179R"

<400>  86
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gtg cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ggt ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Gly Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  87
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  87

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Gly Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  88
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZuFmEgD9ES-A21V/L35G/L108G/I179R

<400>  88
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gtgcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gggtttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  89
<211>  4313
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKUM

<400>  89
taatcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc       60

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga      120

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg      180

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg      240

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg      300

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga      360

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg      420

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag      480

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc      540

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg      600

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt      660

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc      720

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc      780

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg      840

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca      900

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc      960

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     1020

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     1080

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     1140

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     1200

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     1260

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     1320

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     1380

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     1440

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     1500

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     1560

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     1620

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     1680

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     1740

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     1800

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     1860

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     1920

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     1980

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     2040

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     2100

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     2160

cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     2220

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     2280

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     2340

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     2400

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     2460

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     2520

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     2580

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc     2640

cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc     2700

agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc     2760

agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat     2820

tgggtaccgg gccccccctc gaggtcgacg agtatctgtc tgactcgtca ttgccgcctt     2880

tggagtacga ctccaactat gagtgtgctt ggatcacttt gacgatacat tcttcgttgg     2940

aggctgtggg tctgacagct gcgttttcgg cgcggttggc cgacaacaat atcagctgca     3000

acgtcattgc tggctttcat catgatcaca tttttgtcgg caaaggcgac gcccagagag     3060

ccattgacgt tctttctaat ttggaccgat agccgtatag tccagtctat ctataagttc     3120

aactaactcg taactattac cataacatat acttcactgc cccagataag gttccgataa     3180

aaagttctgc agactaaatt tatttcagtc tcctcttcac caccaaaatg ccctcctacg     3240

aagctcgagt gctcaagctc gtggcagcca agaaaaccaa cctgtgtgct tctctggatg     3300

ttaccaccac caaggagctc attgagcttg ccgataaggt cggaccttat gtgtgcatga     3360

tcaaaaccca tatcgacatc attgacgact tcacctacgc cggcactgtg ctccccctca     3420

aggaacttgc tcttaagcac ggtttcttcc tgttcgagga cagaaagttc gcagatattg     3480

gcaacactgt caagcaccag taccggtgtc accgaatcgc cgagtggtcc gatatcacca     3540

acgcccacgg tgtacccgga accggaatcg attgctggcc tgcgagctgg tgcgtacgag     3600

gaaactgtct ctgaacagaa gaaggaggac gtctctgact acgagaactc ccagtacaag     3660

gagttcctag tcccctctcc caacgagaag ctggccagag gtctgctcat gctggccgag     3720

ctgtcttgca agggctctct ggccactggc gagtactcca agcagaccat tgagcttgcc     3780

cgatccgacc ccgagtttgt ggttggcttc attgcccaga accgacctaa gggcgactct     3840

gaggactggc ttattctgac ccccggggtg ggtcttgacg acaagggaga cgctctcgga     3900

cagcagtacc gaactgttga ggatgtcatg tctaccggaa cggatatcat aattgtcggc     3960

cgaggtctgt acggccagaa ccgagatcct attgaggagg ccaagcgata ccagaaggct     4020

ggctgggagg cttaccagaa gattaactgt tagaggttag actatggata tgtaatttaa     4080

ctgtgtatat agagagcgtg caagtatgga gcgcttgttc agcttgtatg atggtcagac     4140

gacctgtctg atcgagtatg tatgatactg cacaacctgt gtatccgcat gatctgtcca     4200

atggggcatg ttgttgtgtt tctcgatacg gagatgctgg gtacagtgct aatacgttga     4260

actacttata cttatatgag gctcgaagaa agctgacttg tgtatgactt aat            4313


<210>  90
<211>  13565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pZKL3-9DP9N

<400>  90
gtacggattg tgtatgtccc tgtacctgca tcttgatgga gagagctccg gaaagcggat       60

caggagctgt ccaattttaa ttttataaca tggaaacgag tccttggagc tagaagacca      120

ttttttcaac tgccctatcg actatattta tctactccaa aaccgactgc ttcccaagaa      180

tcttcagcca aggcttccaa agtaacccct cgcttcccga cacttaattg aaaccttaga      240

tgcagtcact gcgagtgaag tggactctaa catctccaac atagcgacga tattgcgagg      300

gtttgaatat aactaagatg catgatccat tacatttgta gaaatatcat aaacaacgaa      360

gcacatagac agaatgctgt tggttgttac atctgaagcc gaggtaccga tgtcattttc      420

agctgtcact gcagagacag gggtatgtca catttgaaga tcatacaacc gacgtttatg      480

aaaaccagag atatagagaa tgtattgacg gttgtggcta tgtcataagt gcagtgaagt      540

gcagtgatta taggtatagt acacttactg tagctacaag tacatactgc tacagtaata      600

ctcatgtatg caaaccgtat tctgtgtcta cagaaggcga tacggaagag tcaatctctt      660

atgtagagcc atttctataa tcgaaggggc cttgtaattt ccaaacgagt aattgagtaa      720

ttgaagagca tcgtagacat tacttatcat gtattgtgag agggaggaga tgcagctgta      780

gctactgcac atactgtact cgcccatgca gggataatgc atagcgagac ttggcagtag      840

gtgacagttg ctagctgcta cttgtagtcg ggtgggtgat agcatggcgc gccagctgca      900

ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc      960

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     1020

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     1080

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     1140

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     1200

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     1260

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     1320

ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     1380

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     1440

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     1500

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     1560

ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     1620

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     1680

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     1740

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     1800

atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta     1860

aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat     1920

ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac     1980

tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg     2040

ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag     2100

tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt     2160

aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt     2220

gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt     2280

tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt     2340

cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct     2400

tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt     2460

ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac     2520

cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa     2580

actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa     2640

ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca     2700

aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct     2760

ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga     2820

atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc     2880

tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt     2940

aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa     3000

ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt     3060

gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa     3120

agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag     3180

ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt     3240

tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg     3300

agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc     3360

cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg     3420

gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct     3480

gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg     3540

gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgcagga     3600

atagacatct tcaataggag cattaatacc tgtgggatca ctgatgtaaa cttctcccag     3660

agtatgtgaa taaccagcgg gccatccaac aaagaagtcg ttccagtgag tgactcggta     3720

catccgtctt tcggggttga tggtaagtcc gtcgtctcct tgcttaaaga acagagcgtc     3780

cacgtagtct gcaaaagcct tgtttccaag tcgaggctgc ccatagttga ttagcgttgg     3840

atcatatcca agattcttca ggttgatgcc catgaataga gcagtgacag ctcctagaga     3900

gtggccagtt acgatcaatt tgtagtcagt gttgtttcca aggaagtcga ccagacgatc     3960

ctgtacgttc accatagtct ctctgtatgc cttctgaaag ccatcatgaa cttggcagcc     4020

aggacaattg atactggcag aagggtttgt ggagtttatg tcagtagtgt taagaggagg     4080

gatactggtc atgtagggtt gttggatcgt ttggatgtca gtaatagcgt ctgcaatgga     4140

gaaagtgcct cggaaaacaa tatacttttc ctttttggtg tgatcgtggg ccaaaaatcc     4200

agtaactgaa gtcgagaaga aatttcctcc aaactggtag tcaagagtca catcgggaaa     4260

atgagcgcaa gagtttccac aggtaaaatc gctctgcagg gcaaatgggc caggggctct     4320

gacacaatag gccacgttag atagccatcc gtacttgaga acaaagtcgt atgtctcctg     4380

ggtgatagga gccgttaatt aagttgcgac acatgtcttg atagtatctt gaattctctc     4440

tcttgagctt ttccataaca agttcttctg cctccaggaa gtccatgggt ggtttgatca     4500

tggttttggt gtagtggtag tgcagtggtg gtattgtgac tggggatgta gttgagaata     4560

agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt caacgtatta     4620

gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt ggacagatca     4680

tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg tctgaccatc     4740

atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt taaattacat     4800

atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca gccttctggt     4860

atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg ccgacaatta     4920

tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt ccgagagcgt     4980

ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca gagtcgccct     5040

taggtcggtt ctgggcaatg aagccaacca caaactcggg gtcggatcgg gcaagctcaa     5100

tggtctgctt ggagtactcg ccagtggcca gagagccctt gcaagacagc tcggccagca     5160

tgagcagacc tctggccagc ttctcgttgg gagaggggac taggaactcc ttgtactggg     5220

agttctcgta gtcagagacg tcctccttct tctgttcaga gacagtttcc tcggcaccag     5280

ctcgcaggcc agcaatgatt ccggttccgg gtacaccgtg ggcgttggtg atatcggacc     5340

actcggcgat tcggtgacac cggtactggt gcttgacagt gttgccaata tctgcgaact     5400

ttctgtcctc gaacaggaag aaaccgtgct taagagcaag ttccttgagg gggagcacag     5460

tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt tttgatcatg cacacataag     5520

gtccgacctt atcggcaagc tcaatgagct ccttggtggt ggtaacatcc agagaagcac     5580

acaggttggt tttcttggct gccacgagct tgagcactcg agcggcaaag gcggacttgt     5640

ggacgttagc tcgagcttcg taggagggca ttttggtggt gaagaggaga ctgaaataaa     5700

tttagtctgc agaacttttt atcggaacct tatctggggc agtgaagtat atgttatggt     5760

aatagttacg agttagttga acttatagat agactggact atacggctat cggtccaaat     5820

tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc gacaaaaatg tgatcatgat     5880

gaaagccagc aatgacgttg cagctgatat tgttgtcggc caaccgcgcc gaaaacgcag     5940

ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa agtgatccaa gcacactcat     6000

agttggagtc gtactccaaa ggcggcaatg acgagtcaga cagatactcg tcgacctttt     6060

ccttgggaac caccaccgtc agcccttctg actcacgtat tgtagccacc gacacaggca     6120

acagtccgtg gatagcagaa tatgtcttgt cggtccattt ctcaccaact ttaggcgtca     6180

agtgaatgtt gcagaagaag tatgtgcctt cattgagaat cggtgttgct gatttcaata     6240

aagtcttgag atcagtttgg ccagtcatgt tgtggggggt aattggattg agttatcgcc     6300

tacagtctgt acaggtatac tcgctgccca ctttatactt tttgattccg ctgcacttga     6360

agcaatgtcg tttaccaaaa gtgagaatgc tccacagaac acaccccagg gtatggttga     6420

gcaaaaaata aacactccga tacggggaat cgaaccccgg tctccacggt tctcaagaag     6480

tattcttgat gagagcgtat cgatggttaa tgctgctgtg tgctgtgtgt gtgtgttgtt     6540

tggcgctcat tgttgcgtta tgcagcgtac accacaatat tggaagctta ttagcctttc     6600

tattttttcg tttgcaaggc ttaacaacat tgctgtggag agggatgggg atatggaggc     6660

cgctggaggg agtcggagag gcgttttgga gcggcttggc ctggcgccca gctcgcgaaa     6720

cgcacctagg accctttggc acgccgaaat gtgccacttt tcagtctagt aacgccttac     6780

ctacgtcatt ccatgcgtgc atgtttgcgc cttttttccc ttgcccttga tcgccacaca     6840

gtacagtgca ctgtacagtg gaggttttgg gggggtctta gatgggagct aaaagcggcc     6900

tagcggtaca ctagtgggat tgtatggagt ggcatggagc ctaggtggag cctgacagga     6960

cgcacgaccg gctagcccgt gacagacgat gggtggctcc tgttgtccac cgcgtacaaa     7020

tgtttgggcc aaagtcttgt cagccttgct tgcgaaccta attcccaatt ttgtcacttc     7080

gcacccccat tgatcgagcc ctaacccctg cccatcaggc aatccaatta agctcgcatt     7140

gtctgccttg tttagtttgg ctcctgcccg tttcggcgtc cacttgcaca aacacaaaca     7200

agcattatat ataaggctcg tctctccctc ccaaccacac tcactttttt gcccgtcttc     7260

ccttgctaac acaaaagtca agaacacaaa caaccacccc aaccccctta cacacaagac     7320

atatctacag caatggccat ggccaaaagc aaacgacggt cggaggctgt ggaagagcac     7380

gtgaccggct cggacgaggg cttgaccgat acttcgggtc acgtgagccc tgccgccaag     7440

aagcagaaga actcggagat tcatttcacc acccaggctg cccagcagtt ggatcgggag     7500

cgcaaggagg agtatctgga ctcgctgatc gacaacaagg actatctcaa gtaccgtcct     7560

cgaggctgga agctcaacaa cccgcctacc gaccgacctg tgcgaatcta cgccgatgga     7620

gtgtttgatt tgttccatct gggacacatg cgtcagctgg agcagtccaa gaaggccttc     7680

cccaacgcag tgttgattgt gggcattccc agcgacaagg agacccacaa gcggaaggga     7740

ttgaccgtgc tgagtgacgt ccagcggtac gagacggtgc gacactgcaa gtgggtggac     7800

gaggtggtgg aggatgctcc ctggtgtgtc accatggact ttctggaaaa acacaaaatc     7860

gactacgtgg cccatgacga tctgccctac gcttccggca acgacgatga tatctacaag     7920

cccatcaagg agaagggcat gtttctggcc acccagcgaa ccgagggcat ttccacctcg     7980

gacatcatca ccaagattat ccgagactac gacaagtatt taatgcgaaa ctttgcccgg     8040

ggtgctaacc gaaaggatct caacgtctcg tggctcaaga agaacgagct ggacttcaag     8100

cgtcatgtgg ccgagttccg aaactcgttc aagcgaaaga aggtcggtaa ggatctctac     8160

ggcgagattc gcggtctgct gcagaatgtg ctcatttgga acggcgacaa ctccggcact     8220

tccactcccc agcgaaagac gctgcagacc aacgccaaga agatgtacat gaacgtgctc     8280

aagactctgc aggctcctga cgctgttgac gtggactcct cggagaacgt gtctgagaac     8340

gtcactgatg aggaggagga agacgacgac gaggttgatg aggacgaaga agccgacgac     8400

gacgacgaag acgacgaaga cgaggaagac gacgagtagg cggccgcatt gatgattgga     8460

aacacacaca tgggttatat ctaggtgaga gttagttgga cagttatata ttaaatcagc     8520

tatgccaacg gtaacttcat tcatgtcaac gaggaaccag tgactgcaag taatatagaa     8580

tttgaccacc ttgccattct cttgcactcc tttactatat ctcatttatt tcttatatac     8640

aaatcacttc ttcttcccag catcgagctc ggaaacctca tgagcaataa catcgtggat     8700

ctcgtcaata gagggctttt tggactcctt gctgttggcc accttgtcct tgctgtttaa     8760

acacgcagta ggatgtcctg cacgggtctt tttgtggggt gtggagaaag gggtgcttgg     8820

agatggaagc cggtagaacc gggctgcttg tgcttggaga tggaagccgg tagaaccggg     8880

ctgcttgggg ggatttgggg ccgctgggct ccaaagaggg gtaggcattt cgttggggtt     8940

acgtaattgc ggcatttggg tcctgcgcgc atgtcccatt ggtcagaatt agtccggata     9000

ggagacttat cagccaatca cagcgccgga tccacctgta ggttgggttg ggtgggagca     9060

cccctccaca gagtagagtc aaacagcagc agcaacatga tagttggggg tgtgcgtgtt     9120

aaaggaaaaa aaagaagctt gggttatatt cccgctctat ttagaggttg cgggatagac     9180

gccgacggag ggcaatggcg ctatggaacc ttgcggatat ccatacgccg cggcggactg     9240

cgtccgaacc agctccagca gcgttttttc cgggccattg agccgactgc gaccccgcca     9300

acgtgtcttg gcccacgcac tcatgtcatg ttggtgttgg gaggccactt tttaagtagc     9360

acaaggcacc tagctcgcag caaggtgtcc gaaccaaaga agcggctgca gtggtgcaaa     9420

cggggcggaa acggcgggaa aaagccacgg gggcacgaat tgaggcacgc cctcgaattt     9480

gagacgagtc acggccccat tcgcccgcgc aatggctcgc caacgcccgg tcttttgcac     9540

cacatcaggt taccccaagc caaacctttg tgttaaaaag cttaacatat tataccgaac     9600

gtaggtttgg gcgggcttgc tccgtctgtc caaggcaaca tttatataag ggtctgcatc     9660

gccggctcaa ttgaatcttt tttcttcttc tcttctctat attcattctt gaattaaaca     9720

cacatcaaca tggccatcaa agtcggtatt aacggattcg ggcgaatcgg acgaattgtg     9780

agtaccatag aaggtgatgg aaacatgacc caacagaaac agatgacaag tgtcatcgac     9840

ccaccagagc ccaattgagc tcatactaac agtcgacaac ctgtcgaacc aattgatgac     9900

tccccgacaa tgtactaaca caggtcctgc ccatggtgaa aaacgtggac caagtggatc     9960

tctcgcaggt cgacaccatt gcctccggcc gagatgtcaa ctacaaggtc aagtacacct    10020

ccggcgttaa gatgagccag ggcgcctacg acgacaaggg ccgccacatt tccgagcagc    10080

ccttcacctg ggccaactgg caccagcaca tcaactggct caacttcatt ctggtgattg    10140

cgctgcctct gtcgtccttt gctgccgctc ccttcgtctc cttcaactgg aagaccgccg    10200

cgtttgctgt cggctattac atgtgcaccg gtctcggtat caccgccggc taccaccgaa    10260

tgtgggccca tcgagcctac aaggccgctc tgcccgttcg aatcatcctt gctctgtttg    10320

gaggaggagc tgtcgagggc tccatccgat ggtgggcctc gtctcaccga gtccaccacc    10380

gatggaccga ctccaacaag gacccttacg acgcccgaaa gggattctgg ttctcccact    10440

ttggctggat gctgcttgtg cccaacccca agaacaaggg ccgaactgac atttctgacc    10500

tcaacaacga ctgggttgtc cgactccagc acaagtacta cgtttacgtt ctcgtcttca    10560

tggccattgt tctgcccacc ctcgtctgtg gctttggctg gggcgactgg aagggaggtc    10620

ttgtctacgc cggtatcatg cgatacacct ttgtgcagca ggtgactttc tgtgtcaact    10680

cccttgccca ctggattgga gagcagccct tcgacgaccg acgaactccc cgagaccacg    10740

ctcttaccgc cctggtcacc tttggagagg gctaccacaa cttccaccac gagttcccct    10800

cggactaccg aaacgccctc atctggtacc agtacgaccc caccaagtgg ctcatctgga    10860

ccctcaagca ggttggtctc gcctgggacc tccagacctt ctcccagaac gccatcgagc    10920

agggtctcgt gcagcagcga cagaagaagc tggacaagtg gcgaaacaac ctcaactggg    10980

gtatccccat tgagcagctg cctgtcattg agtttgagga gttccaagag caggccaaga    11040

cccgagatct ggttctcatt tctggcattg tccacgacgt gtctgccttt gtcgagcacc    11100

accctggtgg aaaggccctc attatgagcg ccgtcggcaa ggacggtacc gctgtcttca    11160

acggaggtgt ctaccgacac tccaacgctg gccacaacct gcttgccacc atgcgagttt    11220

cggtcattcg aggcggcatg gaggttgagg tgtggaagac tgcccagaac gaaaagaagg    11280

accagaacat tgtctccgat gagagtggaa accgaatcca ccgagctggt ctccaggcca    11340

cccgggtcga gaaccccggt atgtctggca tggctgctta ggcggccgca tgagaagata    11400

aatatataaa tacattgaga tattaaatgc gctagattag agagcctcat actgctcgga    11460

gagaagccaa gacgagtact caaaggggat tacaccatcc atatccacag acacaagctg    11520

gggaaaggtt ctatatacac tttccggaat accgtagttt ccgatgttat caatgggggc    11580

agccaggatt tcaggcactt cggtgtctcg gggtgaaatg gcgttcttgg cctccatcaa    11640

gtcgtaccat gtcttcattt gcctgtcaaa gtaaaacaga agcagatgaa gaatgaactt    11700

gaagtgaagg aatttaaata gttggagcaa gggagaaatg tagagtgtga aagactcact    11760

atggtccggg cttatctcga ccaatagcca aagtctggag tttctgagag aaaaaggcaa    11820

gatacgtatg taacaaagcg acgcatggta caataatacc ggaggcatgt atcatagaga    11880

gttagtggtt cgatgatggc actggtgcct ggtatgactt tatacggctg actacatatt    11940

tgtcctcaga catacaatta cagtcaagca cttacccttg gacatctgta ggtacccccc    12000

ggccaagacg atctcagcgt gtcgtatgtc ggattggcgt agctccctcg ctcgtcaatt    12060

ggctcccatc tactttcttc tgcttggcta cacccagcat gtctgctatg gctcgttttc    12120

gtgccttatc tatcctccca gtattaccaa ctctaaatga catgatgtga ttgggtctac    12180

actttcatat cagagataag gagtagcaca gttgcataaa aagcccaact ctaatcagct    12240

tcttcctttc ttgtaattag tacaaaggtg attagcgaaa tctggaagct tagttggccc    12300

taaaaaaatc aaaaaaagca aaaaacgaaa aacgaaaaac cacagttttg agaacaggga    12360

ggtaacgaag gatcgtatat atatatatat atatatatac ccacggatcc cgagaccggc    12420

ctttgattct tccctacaac caaccattct caccacccta attcacaacc atggaggtcg    12480

tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat gctcagctct    12540

ggtctgatgc ctcgcactgc gaggtgctgt acctctccat cgccttcgtc atcctgaagt    12600

tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg ttcaccaact    12660

acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc tacgccatgt    12720

acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat gtcttccgaa    12780

tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc ttctatctgc    12840

ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga gctcctatgg    12900

acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg ctgctcaacg    12960

gcttcattca ctggatcatg tacggctact attggacccg actgatcaag ctcaagttcc    13020

ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt ggcttctaca    13080

tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga atgtttggct    13140

ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac ttctacgtgc    13200

agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtgagcg gccgcaagtg    13260

tggatgggga agtgagtgcc cggttctgtg tgcacaattg gcaatccaag atggatggat    13320

tcaacacagg gatatagcga gctacgtggt ggtgcgagga tatagcaacg gatatttatg    13380

tttgacactt gagaatgtac gatacaagca ctgtccaagt acaatactaa acatactgta    13440

catactcata ctcgtacccg gcaacggttt cacttgagtg cagtggctag tgctcttact    13500

cgtacagtgt gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta    13560

gttgc                                                                13565


<210>  91
<211>  1449
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1449)
<223>  delta-9 desaturase; GenBank Accession No. XM_501496

<400>  91
atg gtg aaa aac gtg gac caa gtg gat ctc tcg cag gtc gac acc att         48
Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile           
1               5                   10                  15                

gcc tcc ggc cga gat gtc aac tac aag gtc aag tac acc tcc ggc gtt         96
Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val           
            20                  25                  30                    

aag atg agc cag ggc gcc tac gac gac aag ggc cgc cac att tcc gag        144
Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu           
        35                  40                  45                        

cag ccc ttc acc tgg gcc aac tgg cac cag cac atc aac tgg ctc aac        192
Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn           
    50                  55                  60                            

ttc att ctg gtg att gcg ctg cct ctg tcg tcc ttt gct gcc gct ccc        240
Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro           
65                  70                  75                  80            

ttc gtc tcc ttc aac tgg aag acc gcc gcg ttt gct gtc ggc tat tac        288
Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr           
                85                  90                  95                

atg tgc acc ggt ctc ggt atc acc gcc ggc tac cac cga atg tgg gcc        336
Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala           
            100                 105                 110                   

cat cga gcc tac aag gcc gct ctg ccc gtt cga atc atc ctt gct ctg        384
His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu           
        115                 120                 125                       

ttt gga gga gga gct gtc gag ggc tcc atc cga tgg tgg gcc tcg tct        432
Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser           
    130                 135                 140                           

cac cga gtc cac cac cga tgg acc gac tcc aac aag gac cct tac gac        480
His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp           
145                 150                 155                 160           

gcc cga aag gga ttc tgg ttc tcc cac ttt ggc tgg atg ctg ctt gtg        528
Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val           
                165                 170                 175               

ccc aac ccc aag aac aag ggc cga act gac att tct gac ctc aac aac        576
Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn           
            180                 185                 190                   

gac tgg gtt gtc cga ctc cag cac aag tac tac gtt tac gtt ctc gtc        624
Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val           
        195                 200                 205                       

ttc atg gcc att gtt ctg ccc acc ctc gtc tgt ggc ttt ggc tgg ggc        672
Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly           
    210                 215                 220                           

gac tgg aag gga ggt ctt gtc tac gcc ggt atc atg cga tac acc ttt        720
Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe           
225                 230                 235                 240           

gtg cag cag gtg act ttc tgt gtc aac tcc ctt gcc cac tgg att gga        768
Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly           
                245                 250                 255               

gag cag ccc ttc gac gac cga cga act ccc cga gac cac gct ctt acc        816
Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr           
            260                 265                 270                   

gcc ctg gtc acc ttt gga gag ggc tac cac aac ttc cac cac gag ttc        864
Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe           
        275                 280                 285                       

ccc tcg gac tac cga aac gcc ctc atc tgg tac cag tac gac ccc acc        912
Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr           
    290                 295                 300                           

aag tgg ctc atc tgg acc ctc aag cag gtt ggt ctc gcc tgg gac ctc        960
Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu           
305                 310                 315                 320           

cag acc ttc tcc cag aac gcc atc gag cag ggt ctc gtg cag cag cga       1008
Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg           
                325                 330                 335               

cag aag aag ctg gac aag tgg cga aac aac ctc aac tgg ggt atc ccc       1056
Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro           
            340                 345                 350                   

att gag cag ctg cct gtc att gag ttt gag gag ttc caa gag cag gcc       1104
Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala           
        355                 360                 365                       

aag acc cga gat ctg gtt ctc att tct ggc att gtc cac gac gtg tct       1152
Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser           
    370                 375                 380                           

gcc ttt gtc gag cac cac cct ggt gga aag gcc ctc att atg agc gcc       1200
Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala           
385                 390                 395                 400           

gtc ggc aag gac ggt acc gct gtc ttc aac gga ggt gtc tac cga cac       1248
Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His           
                405                 410                 415               

tcc aac gct ggc cac aac ctg ctt gcc acc atg cga gtt tcg gtc att       1296
Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile           
            420                 425                 430                   

cga ggc ggc atg gag gtt gag gtg tgg aag act gcc cag aac gaa aag       1344
Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys           
        435                 440                 445                       

aag gac cag aac att gtc tcc gat gag agt gga aac cga atc cac cga       1392
Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg           
    450                 455                 460                           

gct ggt ctc cag gcc acc cgg gtc gag aac ccc ggt atg tct ggc atg       1440
Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met           
465                 470                 475                 480           

gct gct tag                                                           1449
Ala Ala                                                                   
                                                                          


<210>  92
<211>  482
<212>  PRT
<213>  Yarrowia lipolytica

<400>  92

Met Val Lys Asn Val Asp Gln Val Asp Leu Ser Gln Val Asp Thr Ile 
1               5                   10                  15      


Ala Ser Gly Arg Asp Val Asn Tyr Lys Val Lys Tyr Thr Ser Gly Val 
            20                  25                  30          


Lys Met Ser Gln Gly Ala Tyr Asp Asp Lys Gly Arg His Ile Ser Glu 
        35                  40                  45              


Gln Pro Phe Thr Trp Ala Asn Trp His Gln His Ile Asn Trp Leu Asn 
    50                  55                  60                  


Phe Ile Leu Val Ile Ala Leu Pro Leu Ser Ser Phe Ala Ala Ala Pro 
65                  70                  75                  80  


Phe Val Ser Phe Asn Trp Lys Thr Ala Ala Phe Ala Val Gly Tyr Tyr 
                85                  90                  95      


Met Cys Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Met Trp Ala 
            100                 105                 110         


His Arg Ala Tyr Lys Ala Ala Leu Pro Val Arg Ile Ile Leu Ala Leu 
        115                 120                 125             


Phe Gly Gly Gly Ala Val Glu Gly Ser Ile Arg Trp Trp Ala Ser Ser 
    130                 135                 140                 


His Arg Val His His Arg Trp Thr Asp Ser Asn Lys Asp Pro Tyr Asp 
145                 150                 155                 160 


Ala Arg Lys Gly Phe Trp Phe Ser His Phe Gly Trp Met Leu Leu Val 
                165                 170                 175     


Pro Asn Pro Lys Asn Lys Gly Arg Thr Asp Ile Ser Asp Leu Asn Asn 
            180                 185                 190         


Asp Trp Val Val Arg Leu Gln His Lys Tyr Tyr Val Tyr Val Leu Val 
        195                 200                 205             


Phe Met Ala Ile Val Leu Pro Thr Leu Val Cys Gly Phe Gly Trp Gly 
    210                 215                 220                 


Asp Trp Lys Gly Gly Leu Val Tyr Ala Gly Ile Met Arg Tyr Thr Phe 
225                 230                 235                 240 


Val Gln Gln Val Thr Phe Cys Val Asn Ser Leu Ala His Trp Ile Gly 
                245                 250                 255     


Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro Arg Asp His Ala Leu Thr 
            260                 265                 270         


Ala Leu Val Thr Phe Gly Glu Gly Tyr His Asn Phe His His Glu Phe 
        275                 280                 285             


Pro Ser Asp Tyr Arg Asn Ala Leu Ile Trp Tyr Gln Tyr Asp Pro Thr 
    290                 295                 300                 


Lys Trp Leu Ile Trp Thr Leu Lys Gln Val Gly Leu Ala Trp Asp Leu 
305                 310                 315                 320 


Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu Val Gln Gln Arg 
                325                 330                 335     


Gln Lys Lys Leu Asp Lys Trp Arg Asn Asn Leu Asn Trp Gly Ile Pro 
            340                 345                 350         


Ile Glu Gln Leu Pro Val Ile Glu Phe Glu Glu Phe Gln Glu Gln Ala 
        355                 360                 365             


Lys Thr Arg Asp Leu Val Leu Ile Ser Gly Ile Val His Asp Val Ser 
    370                 375                 380                 


Ala Phe Val Glu His His Pro Gly Gly Lys Ala Leu Ile Met Ser Ala 
385                 390                 395                 400 


Val Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly Val Tyr Arg His 
                405                 410                 415     


Ser Asn Ala Gly His Asn Leu Leu Ala Thr Met Arg Val Ser Val Ile 
            420                 425                 430         


Arg Gly Gly Met Glu Val Glu Val Trp Lys Thr Ala Gln Asn Glu Lys 
        435                 440                 445             


Lys Asp Gln Asn Ile Val Ser Asp Glu Ser Gly Asn Arg Ile His Arg 
    450                 455                 460                 


Ala Gly Leu Gln Ala Thr Arg Val Glu Asn Pro Gly Met Ser Gly Met 
465                 470                 475                 480 


Ala Ala 
        


<210>  93
<211>  1101
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  CDS
<222>  (1)..(1101)
<223>  choline-phosphate cytidylyl-transferase; GenBank Accession No. 
       XM_502978

<400>  93
atg gcc aaa agc aaa cga cgg tcg gag gct gtg gaa gag cac gtg acc         48
Met Ala Lys Ser Lys Arg Arg Ser Glu Ala Val Glu Glu His Val Thr           
1               5                   10                  15                

ggc tcg gac gag ggc ttg acc gat act tcg ggt cac gtg agc cct gcc         96
Gly Ser Asp Glu Gly Leu Thr Asp Thr Ser Gly His Val Ser Pro Ala           
            20                  25                  30                    

gcc aag aag cag aag aac tcg gag att cat ttc acc acc cag gct gcc        144
Ala Lys Lys Gln Lys Asn Ser Glu Ile His Phe Thr Thr Gln Ala Ala           
        35                  40                  45                        

cag cag ttg gat cgg gag cgc aag gag gag tat ctg gac tcg ctg atc        192
Gln Gln Leu Asp Arg Glu Arg Lys Glu Glu Tyr Leu Asp Ser Leu Ile           
    50                  55                  60                            

gac aac aag gac tat ctc aag tac cgt cct cga ggc tgg aag ctc aac        240
Asp Asn Lys Asp Tyr Leu Lys Tyr Arg Pro Arg Gly Trp Lys Leu Asn           
65                  70                  75                  80            

aac ccg cct acc gac cga cct gtg cga atc tac gcc gat gga gtg ttt        288
Asn Pro Pro Thr Asp Arg Pro Val Arg Ile Tyr Ala Asp Gly Val Phe           
                85                  90                  95                

gat ttg ttc cat ctg gga cac atg cgt cag ctg gag cag tcc aag aag        336
Asp Leu Phe His Leu Gly His Met Arg Gln Leu Glu Gln Ser Lys Lys           
            100                 105                 110                   

gcc ttc ccc aac gca gtg ttg att gtg ggc att ccc agc gac aag gag        384
Ala Phe Pro Asn Ala Val Leu Ile Val Gly Ile Pro Ser Asp Lys Glu           
        115                 120                 125                       

acc cac aag cgg aag gga ttg acc gtg ctg agt gac gtc cag cgg tac        432
Thr His Lys Arg Lys Gly Leu Thr Val Leu Ser Asp Val Gln Arg Tyr           
    130                 135                 140                           

gag acg gtg cga cac tgc aag tgg gtg gac gag gtg gtg gag gat gct        480
Glu Thr Val Arg His Cys Lys Trp Val Asp Glu Val Val Glu Asp Ala           
145                 150                 155                 160           

ccc tgg tgt gtc acc atg gac ttt ctg gaa aaa cac aaa atc gac tac        528
Pro Trp Cys Val Thr Met Asp Phe Leu Glu Lys His Lys Ile Asp Tyr           
                165                 170                 175               

gtg gcc cat gac gat ctg ccc tac gct tcc ggc aac gac gat gat atc        576
Val Ala His Asp Asp Leu Pro Tyr Ala Ser Gly Asn Asp Asp Asp Ile           
            180                 185                 190                   

tac aag ccc atc aag gag aag ggc atg ttt ctg gcc acc cag cga acc        624
Tyr Lys Pro Ile Lys Glu Lys Gly Met Phe Leu Ala Thr Gln Arg Thr           
        195                 200                 205                       

gag ggc att tcc acc tcg gac atc atc acc aag att atc cga gac tac        672
Glu Gly Ile Ser Thr Ser Asp Ile Ile Thr Lys Ile Ile Arg Asp Tyr           
    210                 215                 220                           

gac aag tat tta atg cga aac ttt gcc cgg ggt gct aac cga aag gat        720
Asp Lys Tyr Leu Met Arg Asn Phe Ala Arg Gly Ala Asn Arg Lys Asp           
225                 230                 235                 240           

ctc aac gtc tcg tgg ctc aag aag aac gag ctg gac ttc aag cgt cat        768
Leu Asn Val Ser Trp Leu Lys Lys Asn Glu Leu Asp Phe Lys Arg His           
                245                 250                 255               

gtg gcc gag ttc cga aac tcg ttc aag cga aag aag gtc ggt aag gat        816
Val Ala Glu Phe Arg Asn Ser Phe Lys Arg Lys Lys Val Gly Lys Asp           
            260                 265                 270                   

ctc tac ggc gag att cgc ggt ctg ctg cag aat gtg ctc att tgg aac        864
Leu Tyr Gly Glu Ile Arg Gly Leu Leu Gln Asn Val Leu Ile Trp Asn           
        275                 280                 285                       

ggc gac aac tcc ggc act tcc act ccc cag cga aag acg ctg cag acc        912
Gly Asp Asn Ser Gly Thr Ser Thr Pro Gln Arg Lys Thr Leu Gln Thr           
    290                 295                 300                           

aac gcc aag aag atg tac atg aac gtg ctc aag act ctg cag gct cct        960
Asn Ala Lys Lys Met Tyr Met Asn Val Leu Lys Thr Leu Gln Ala Pro           
305                 310                 315                 320           

gac gct gtt gac gtg gac tcc tcg gag aac gtg tct gag aac gtc act       1008
Asp Ala Val Asp Val Asp Ser Ser Glu Asn Val Ser Glu Asn Val Thr           
                325                 330                 335               

gat gag gag gag gaa gac gac gac gag gtt gat gag gac gaa gaa gcc       1056
Asp Glu Glu Glu Glu Asp Asp Asp Glu Val Asp Glu Asp Glu Glu Ala           
            340                 345                 350                   

gac gac gac gac gaa gac gac gaa gac gag gaa gac gac gag tag           1101
Asp Asp Asp Asp Glu Asp Asp Glu Asp Glu Glu Asp Asp Glu                   
        355                 360                 365                       


<210>  94
<211>  366
<212>  PRT
<213>  Yarrowia lipolytica

<400>  94

Met Ala Lys Ser Lys Arg Arg Ser Glu Ala Val Glu Glu His Val Thr 
1               5                   10                  15      


Gly Ser Asp Glu Gly Leu Thr Asp Thr Ser Gly His Val Ser Pro Ala 
            20                  25                  30          


Ala Lys Lys Gln Lys Asn Ser Glu Ile His Phe Thr Thr Gln Ala Ala 
        35                  40                  45              


Gln Gln Leu Asp Arg Glu Arg Lys Glu Glu Tyr Leu Asp Ser Leu Ile 
    50                  55                  60                  


Asp Asn Lys Asp Tyr Leu Lys Tyr Arg Pro Arg Gly Trp Lys Leu Asn 
65                  70                  75                  80  


Asn Pro Pro Thr Asp Arg Pro Val Arg Ile Tyr Ala Asp Gly Val Phe 
                85                  90                  95      


Asp Leu Phe His Leu Gly His Met Arg Gln Leu Glu Gln Ser Lys Lys 
            100                 105                 110         


Ala Phe Pro Asn Ala Val Leu Ile Val Gly Ile Pro Ser Asp Lys Glu 
        115                 120                 125             


Thr His Lys Arg Lys Gly Leu Thr Val Leu Ser Asp Val Gln Arg Tyr 
    130                 135                 140                 


Glu Thr Val Arg His Cys Lys Trp Val Asp Glu Val Val Glu Asp Ala 
145                 150                 155                 160 


Pro Trp Cys Val Thr Met Asp Phe Leu Glu Lys His Lys Ile Asp Tyr 
                165                 170                 175     


Val Ala His Asp Asp Leu Pro Tyr Ala Ser Gly Asn Asp Asp Asp Ile 
            180                 185                 190         


Tyr Lys Pro Ile Lys Glu Lys Gly Met Phe Leu Ala Thr Gln Arg Thr 
        195                 200                 205             


Glu Gly Ile Ser Thr Ser Asp Ile Ile Thr Lys Ile Ile Arg Asp Tyr 
    210                 215                 220                 


Asp Lys Tyr Leu Met Arg Asn Phe Ala Arg Gly Ala Asn Arg Lys Asp 
225                 230                 235                 240 


Leu Asn Val Ser Trp Leu Lys Lys Asn Glu Leu Asp Phe Lys Arg His 
                245                 250                 255     


Val Ala Glu Phe Arg Asn Ser Phe Lys Arg Lys Lys Val Gly Lys Asp 
            260                 265                 270         


Leu Tyr Gly Glu Ile Arg Gly Leu Leu Gln Asn Val Leu Ile Trp Asn 
        275                 280                 285             


Gly Asp Asn Ser Gly Thr Ser Thr Pro Gln Arg Lys Thr Leu Gln Thr 
    290                 295                 300                 


Asn Ala Lys Lys Met Tyr Met Asn Val Leu Lys Thr Leu Gln Ala Pro 
305                 310                 315                 320 


Asp Ala Val Asp Val Asp Ser Ser Glu Asn Val Ser Glu Asn Val Thr 
                325                 330                 335     


Asp Glu Glu Glu Glu Asp Asp Asp Glu Val Asp Glu Asp Glu Glu Ala 
            340                 345                 350         


Asp Asp Asp Asp Glu Asp Asp Glu Asp Glu Glu Asp Asp Glu 
        355                 360                 365     


<210>  95
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  95
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat       60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acggatccat cgccttcgtc      120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg      180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc      240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat      300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc      360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga      420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg      480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag      540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt      600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga      660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac      720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga         777


<210>  96
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  96
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat       60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acggctccat cgccttcgtc      120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg      180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc      240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat      300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc      360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga      420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg      480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag      540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt      600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga      660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac      720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga         777


<210>  97
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  misc_feature
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G"

<400>  97
atggaggtcg tgaacgaaat cgtctccatt ggccaggagg ttcttcccaa ggtcgactat       60

gctcagctct ggtctgatgc ctcgcactgc gaggtgctgt acggttccat cgccttcgtc      120

atcctgaagt tcacccttgg tcctctcgga cccaagggtc agtctcgaat gaagtttgtg      180

ttcaccaact acaacctgct catgtccatc tactcgctgg gctccttcct ctctatggcc      240

tacgccatgt acaccattgg tgtcatgtcc gacaactgcg agaaggcttt cgacaacaat      300

gtcttccgaa tcaccactca gctgttctac ctcagcaagt tcctcgagta cattgactcc      360

ttctatctgc ccctcatggg caagcctctg acctggttgc agttctttca ccatctcgga      420

gctcctatgg acatgtggct gttctacaac taccgaaacg aagccgtttg gatctttgtg      480

ctgctcaacg gcttcattca ctggatcatg tacggctact attggacccg actgatcaag      540

ctcaagttcc ctatgcccaa gtccctgatt acttctatgc agatcattca gttcaacgtt      600

ggcttctaca tcgtctggaa gtaccggaac attccctgct accgacaaga tggaatgaga      660

atgtttggct ggtttttcaa ctacttctac gttggtactg tcctgtgtct gttcctcaac      720

ttctacgtgc agacctacat cgtccgaaag cacaagggag ccaaaaagat tcagtga         777


<210>  98
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer FBAIN-F

<400>  98
caagcggggg gcttgtctag ggtat                                             25


<210>  99
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Y1026

<400>  99
tttgcggccg ctcactgaat ctttttggct cccttgtgct                             40


<210>  100
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G/W132T/I179R"

<400>  100
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc acc ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  101
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  101

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  102
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-L35G/W132T/I179R

<400>  102
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

accaccttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  103
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-S9D/L35G/Y84C/I179R"

<400>  103
atg gag gtc gtg aac gaa atc gtc gac att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Asp Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tgt acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg cag acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  104
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  104

Met Glu Val Val Asn Glu Ile Val Asp Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Gln Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  105
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-S9D/L35G/Y84C/I179R

<400>  105
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtcgacatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt gtaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtgc agacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  106
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-L35G/Y84C/I179R/Q244N"

<400>  106
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gct cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tgt acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc tgg ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg aac acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  107
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  107

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Ala Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Cys Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Trp Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  108
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-L35G/Y84C/I179R/Q244N

<400>  108
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gctcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt gtaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

acctggttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtga acacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


<210>  109
<211>  777
<212>  DNA
<213>  Euglena gracilis


<220>
<221>  CDS
<222>  (1)..(777)
<223>  mutant delta-9 elongase "EgD9eS-A21V/L35G/W132T/I179R/Q244N"

<400>  109
atg gag gtc gtg aac gaa atc gtc tcc att ggc cag gag gtt ctt ccc         48
Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro           
1               5                   10                  15                

aag gtc gac tat gtg cag ctc tgg tct gat gcc tcg cac tgc gag gtg         96
Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val           
            20                  25                  30                    

ctg tac ggg tcc atc gcc ttc gtc atc ctg aag ttc acc ctt ggt cct        144
Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro           
        35                  40                  45                        

ctc gga ccc aag ggt cag tct cga atg aag ttt gtg ttc acc aac tac        192
Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr           
    50                  55                  60                            

aac ctg ctc atg tcc atc tac tcg ctg ggc tcc ttc ctc tct atg gcc        240
Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala           
65                  70                  75                  80            

tac gcc atg tac acc att ggt gtc atg tcc gac aac tgc gag aag gct        288
Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala           
                85                  90                  95                

ttc gac aac aat gtc ttc cga atc acc act cag ctg ttc tac ctc agc        336
Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser           
            100                 105                 110                   

aag ttc ctc gag tac att gac tcc ttc tat ctg ccc ctc atg ggc aag        384
Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys           
        115                 120                 125                       

cct ctg acc acc ttg cag ttc ttt cac cat ctc gga gct cct atg gac        432
Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp           
    130                 135                 140                           

atg tgg ctg ttc tac aac tac cga aac gaa gcc gtt tgg atc ttt gtg        480
Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val           
145                 150                 155                 160           

ctg ctc aac ggc ttc att cac tgg atc atg tac ggc tac tat tgg acc        528
Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr           
                165                 170                 175               

cga ctg cga aag ctc aag ttc cct atg ccc aag tcc ctg att act tct        576
Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser           
            180                 185                 190                   

atg cag atc att cag ttc aac gtt ggc ttc tac atc gtc tgg aag tac        624
Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr           
        195                 200                 205                       

cgg aac att ccc tgc tac cga caa gat gga atg aga atg ttt ggc tgg        672
Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp           
    210                 215                 220                           

ttt ttc aac tac ttc tac gtt ggt act gtc ctg tgt ctg ttc ctc aac        720
Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn           
225                 230                 235                 240           

ttc tac gtg aac acc tac atc gtc cga aag cac aag gga gcc aaa aag        768
Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys           
                245                 250                 255               

att cag tga                                                            777
Ile Gln                                                                   
                                                                          


<210>  110
<211>  258
<212>  PRT
<213>  Euglena gracilis

<400>  110

Met Glu Val Val Asn Glu Ile Val Ser Ile Gly Gln Glu Val Leu Pro 
1               5                   10                  15      


Lys Val Asp Tyr Val Gln Leu Trp Ser Asp Ala Ser His Cys Glu Val 
            20                  25                  30          


Leu Tyr Gly Ser Ile Ala Phe Val Ile Leu Lys Phe Thr Leu Gly Pro 
        35                  40                  45              


Leu Gly Pro Lys Gly Gln Ser Arg Met Lys Phe Val Phe Thr Asn Tyr 
    50                  55                  60                  


Asn Leu Leu Met Ser Ile Tyr Ser Leu Gly Ser Phe Leu Ser Met Ala 
65                  70                  75                  80  


Tyr Ala Met Tyr Thr Ile Gly Val Met Ser Asp Asn Cys Glu Lys Ala 
                85                  90                  95      


Phe Asp Asn Asn Val Phe Arg Ile Thr Thr Gln Leu Phe Tyr Leu Ser 
            100                 105                 110         


Lys Phe Leu Glu Tyr Ile Asp Ser Phe Tyr Leu Pro Leu Met Gly Lys 
        115                 120                 125             


Pro Leu Thr Thr Leu Gln Phe Phe His His Leu Gly Ala Pro Met Asp 
    130                 135                 140                 


Met Trp Leu Phe Tyr Asn Tyr Arg Asn Glu Ala Val Trp Ile Phe Val 
145                 150                 155                 160 


Leu Leu Asn Gly Phe Ile His Trp Ile Met Tyr Gly Tyr Tyr Trp Thr 
                165                 170                 175     


Arg Leu Arg Lys Leu Lys Phe Pro Met Pro Lys Ser Leu Ile Thr Ser 
            180                 185                 190         


Met Gln Ile Ile Gln Phe Asn Val Gly Phe Tyr Ile Val Trp Lys Tyr 
        195                 200                 205             


Arg Asn Ile Pro Cys Tyr Arg Gln Asp Gly Met Arg Met Phe Gly Trp 
    210                 215                 220                 


Phe Phe Asn Tyr Phe Tyr Val Gly Thr Val Leu Cys Leu Phe Leu Asn 
225                 230                 235                 240 


Phe Tyr Val Asn Thr Tyr Ile Val Arg Lys His Lys Gly Ala Lys Lys 
                245                 250                 255     


Ile Gln 
        


<210>  111
<211>  7769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid EgD9eS-A21V/L35G/W132T/I179R/Q244N

<400>  111
ggccgcaagt gtggatgggg aagtgagtgc ccggttctgt gtgcacaatt ggcaatccaa       60

gatggatgga ttcaacacag ggatatagcg agctacgtgg tggtgcgagg atatagcaac      120

ggatatttat gtttgacact tgagaatgta cgatacaagc actgtccaag tacaatacta      180

aacatactgt acatactcat actcgtaccc gggcaacggt ttcacttgag tgcagtggct      240

agtgctctta ctcgtacagt gtgcaatact gcgtatcata gtctttgatg tatatcgtat      300

tcattcatgt tagttgcgta cgagccggaa gcataaagtg taaagcctgg ggtgcctaat      360

gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc      420

tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      480

ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      540

cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      600

gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      660

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      720

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      780

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      840

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg      900

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      960

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1020

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1080

ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc     1140

cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     1200

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     1260

atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga     1320

ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa     1380

gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa     1440

tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc     1500

ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga     1560

taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa     1620

gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt     1680

gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg     1740

ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc     1800

aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg     1860

gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag     1920

cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt     1980

actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt     2040

caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac     2100

gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac     2160

ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag     2220

caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa     2280

tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga     2340

gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc     2400

cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg     2460

ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct     2520

tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc     2580

ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg     2640

atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt     2700

ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg     2760

tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc     2820

tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc     2880

gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg     2940

ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc     3000

ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga     3060

attgggtacc gggccccccc tcgaggtcga tggtgtcgat aagcttgata tcgaattcat     3120

gtcacacaaa ccgatcttcg cctcaaggaa acctaattct acatccgaga gactgccgag     3180

atccagtcta cactgattaa ttttcgggcc aataatttaa aaaaatcgtg ttatataata     3240

ttatatgtat tatatatata catcatgatg atactgacag tcatgtccca ttgctaaata     3300

gacagactcc atctgccgcc tccaactgat gttctcaata tttaaggggt catctcgcat     3360

tgtttaataa taaacagact ccatctaccg cctccaaatg atgttctcaa aatatattgt     3420

atgaacttat ttttattact tagtattatt agacaactta cttgctttat gaaaaacact     3480

tcctatttag gaaacaattt ataatggcag ttcgttcatt taacaattta tgtagaataa     3540

atgttataaa tgcgtatggg aaatcttaaa tatggatagc ataaatgata tctgcattgc     3600

ctaattcgaa atcaacagca acgaaaaaaa tcccttgtac aacataaata gtcatcgaga     3660

aatatcaact atcaaagaac agctattcac acgttactat tgagattatt attggacgag     3720

aatcacacac tcaactgtct ttctctcttc tagaaataca ggtacaagta tgtactattc     3780

tcattgttca tacttctagt catttcatcc cacatattcc ttggatttct ctccaatgaa     3840

tgacattcta tcttgcaaat tcaacaatta taataagata taccaaagta gcggtatagt     3900

ggcaatcaaa aagcttctct ggtgtgcttc tcgtatttat ttttattcta atgatccatt     3960

aaaggtatat atttatttct tgttatataa tccttttgtt tattacatgg gctggataca     4020

taaaggtatt ttgatttaat tttttgctta aattcaatcc cccctcgttc agtgtcaact     4080

gtaatggtag gaaattacca tacttttgaa gaagcaaaaa aaatgaaaga aaaaaaaaat     4140

cgtatttcca ggttagacgt tccgcagaat ctagaatgcg gtatgcggta cattgttctt     4200

cgaacgtaaa agttgcgctc cctgagatat tgtacatttt tgcttttaca agtacaagta     4260

catcgtacaa ctatgtacta ctgttgatgc atccacaaca gtttgttttg tttttttttg     4320

tttttttttt ttctaatgat tcattaccgc tatgtatacc tacttgtact tgtagtaagc     4380

cgggttattg gcgttcaatt aatcatagac ttatgaatct gcacggtgtg cgctgcgagt     4440

tacttttagc ttatgcatgc tacttgggtg taatattggg atctgttcgg aaatcaacgg     4500

atgctcaatc gatttcgaca gtaattaatt aagtcataca caagtcagct ttcttcgagc     4560

ctcatataag tataagtagt tcaacgtatt agcactgtac ccagcatctc cgtatcgaga     4620

aacacaacaa catgccccat tggacagatc atgcggatac acaggttgtg cagtatcata     4680

catactcgat cagacaggtc gtctgaccat catacaagct gaacaagcgc tccatacttg     4740

cacgctctct atatacacag ttaaattaca tatccatagt ctaacctcta acagttaatc     4800

ttctggtaag cctcccagcc agccttctgg tatcgcttgg cctcctcaat aggatctcgg     4860

ttctggccgt acagacctcg gccgacaatt atgatatccg ttccggtaga catgacatcc     4920

tcaacagttc ggtactgctg tccgagagcg tctcccttgt cgtcaagacc caccccgggg     4980

gtcagaataa gccagtcctc agagtcgccc ttaggtcggt tctgggcaat gaagccaacc     5040

acaaactcgg ggtcggatcg ggcaagctca atggtctgct tggagtactc gccagtggcc     5100

agagagccct tgcaagacag ctcggccagc atgagcagac ctctggccag cttctcgttg     5160

ggagagggga ctaggaactc cttgtactgg gagttctcgt agtcagagac gtcctccttc     5220

ttctgttcag agacagtttc ctcggcacca gctcgcaggc cagcaatgat tccggttccg     5280

ggtacaccgt gggcgttggt gatatcggac cactcggcga ttcggtgaca ccggtactgg     5340

tgcttgacag tgttgccaat atctgcgaac tttctgtcct cgaacaggaa gaaaccgtgc     5400

ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc gtcaatgatg     5460

tcgatatggg ttttgatcat gcacacataa ggtccgacct tatcggcaag ctcaatgagc     5520

tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc tgccacgagc     5580

ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc gtaggagggc     5640

attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt tatcggaacc     5700

ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg aacttataga     5760

tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc tctctgggcg     5820

tcgcctttgc cgacaaaaat gtgatcatga tgaaagccag caatgacgtt gcagctgata     5880

ttgttgtcgg ccaaccgcgc cgaaaacgca gctgtcagac ccacagcctc caacgaagaa     5940

tgtatcgtca aagtgatcca agcacactca tagttggagt cgtactccaa aggcggcaat     6000

gacgagtcag acagatactc gtcgacgttt aaacagtgta cgcagatcta ctatagagga     6060

acatttaaat tgccccggag aagacggcca ggccgcctag atgacaaatt caacaactca     6120

cagctgactt tctgccattg ccactagggg ggggcctttt tatatggcca agccaagctc     6180

tccacgtcgg ttgggctgca cccaacaata aatgggtagg gttgcaccaa caaagggatg     6240

ggatgggggg tagaagatac gaggataacg gggctcaatg gcacaaataa gaacgaatac     6300

tgccattaag actcgtgatc cagcgactga caccattgca tcatctaagg gcctcaaaac     6360

tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     6420

ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     6480

caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     6540

gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     6600

tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     6660

ttttgccttc cgcacatttc cattgctcga tacccacacc ttgcttctcc tgcacttgcc     6720

aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     6780

atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     6840

attcgaaatc taaactacac atcacagaat tccgagccgt gagtatccac gacaagatca     6900

gtgtcgagac gacgcgtttt gtgtaatgac acaatccgaa agtcgctagc aacacacact     6960

ctctacacaa actaacccag ctctggtacc atggaggtcg tgaacgaaat cgtctccatt     7020

ggccaggagg ttcttcccaa ggtcgactat gtgcagctct ggtctgatgc ctcgcactgc     7080

gaggtgctgt acgggtccat cgccttcgtc atcctgaagt tcacccttgg tcctctcgga     7140

cccaagggtc agtctcgaat gaagtttgtg ttcaccaact acaacctgct catgtccatc     7200

tactcgctgg gctccttcct ctctatggcc tacgccatgt acaccattgg tgtcatgtcc     7260

gacaactgcg agaaggcttt cgacaacaat gtcttccgaa tcaccactca gctgttctac     7320

ctcagcaagt tcctcgagta cattgactcc ttctatctgc ccctcatggg caagcctctg     7380

accaccttgc agttctttca ccatctcgga gctcctatgg acatgtggct gttctacaac     7440

taccgaaacg aagccgtttg gatctttgtg ctgctcaacg gcttcattca ctggatcatg     7500

tacggctact attggacccg actgcgaaag ctcaagttcc ctatgcccaa gtccctgatt     7560

acttctatgc agatcattca gttcaacgtt ggcttctaca tcgtctggaa gtaccggaac     7620

attccctgct accgacaaga tggaatgaga atgtttggct ggtttttcaa ctacttctac     7680

gttggtactg tcctgtgtct gttcctcaac ttctacgtga acacctacat cgtccgaaag     7740

cacaagggag ccaaaaagat tcagtgagc                                       7769


