SEQUENCE LISTING 
<110> ZYRUS Beteiligungsgesellsohaf% inbH & Co. Patente 1 KG 
<120> Whole cell biocatalyst 
<130> to be issued 
<160> 4 
<170> PatentIn version 3.5 
<210> 1 
<211> 2790 
<212> DNA 
<213> artificial 
<220> 
<223> construct comprising autotransporter domain and NADH oxidase 
<400> 1 
atggttaaat taaaatttgg tgtttttttt acagttttac tatcttcagc atatgcacat 60 
ggaacacctc aaaatattac tgatttgctc gagaaagtca cagttgttgg ttgtacacat 120 
gccggaacct ttgcgattaa acaaatcttg gccgaacacc ctgatgccga agtgaccgtc 180 
tacgaacgta acgatgtcat ttcatttctc tcttgtggaa tcgcccttta cctgggggga 240 
aaagtcgctg atccgcaagg cctcttttat tcaagtcctg aagaactcca aaaattaggc 300 
gctaatgtcc aaatgaatca caatgtttta gcgatcgatc ctgatcaaaa gacagtgacc 360 
gttgaggact taaccagtca tgcacaaacg actgagtcat acgataaact agtcatgacg 420 
tctggttctt ggccaattgt ccccaagatt ccgggcatcg atagcgatcg cgttaagctc 480 
tgcaaaaact gggcacatge gcaagctcta atcgaagatg ctaaggaagc caagcggatt 540 
accgttattg gtgccggcta tattggtgct gaactagcag aagcctactc cactactggt 600 
catgacgtaa ccttaattga tgcgatggac cgggttatgc ccaagtactt tgatgctgat 660 
tttacggatg tcattgaaca agattatcgg gatcacggtg tccaacttgc cttaagtgaa 720 
acggttgaaa gctttactga tagtgcaact gggttgacca ttaagactga taagaacagc 780 
tatgaaacgg atctcgctat tttatgcatt ggctttagac caaataccga cctgctgaaa 840 
ggcaaagtcg atatggcacc aaacggcgcg attattacgg atgactacat gcgttcttct 900 
aaccctgata ttttcgccgc tggtgacagt gctgctgtgc actacaaccc aacccatcag 960 
aatgcttaca ttcccttagc aaotaacgcg gtgcgtcaag gtatcctagt cggtaaaaac 1020 
ctagttaagc cgaccgtcaa gtatatggga acacaatcat cttctggttt ggcactctat 1080 
gatcggacga tcgtctcaac tggtttaacg ctagcagctg caaaacaaca aggggtgaac 1140 
gctgaacaag tgattgttga agataattat cgcccagagt ttatgccgtc aactgaaccc 1200 
gttttgatgt cattagtctt tgaccccgac acacaccgga tcttaggtgg tgcgttaatg 1260 
agtaatacg atgtttcaca atcggccaac accctttctg tttgcatcca aaacgaaaat 1320 
acaattgatg acttagcgat ggttgatatg ctcttccagc ctaactttga tcgaccattc 1380 
aactacctaa acatcttagc gcaagctgct caggcgaaag ttgcccaatc agttaacgct 1440 
Page 1 
ggtaccctta atcctacaaa agaaagtgca ggtaatactc ttaccgtgtc aaattatact 1500 
gggacaccgg gaagtgttat ttctcttggt ggtgtgcttg aaggagataa ttcacttacg 1560 
gaccgtctgg tggtgaaagg taatacctct ggtcaaagtg acatcgttta tgtcaatgaa 1620 
gatggcagtg gtggtcagac gagagatggt attaatatta tttctgtaga gggaaattct 1680 
gatgcagaat tctctctgaa gaaccgcgta gttgccggag cttatgatta cacactgcag 1740 
aaaggaaacg agagtgggac agataataag ggatggtatt taaccagtca tcttcccaca 1800 
tctgataccc ggcaatacag accggagaac ggaagttatg ctaccaatat ggcactggct 1860 
aactcactgt tcctcatgga tttgaatgag cgtaagcaat tcagggccat gagtgataat 1920 
acacagcctg agtctgcatc cgtgtggatg aagatcactg gaggaataag ctctggtaag 1980 
cttaatgacg ggcaaaataa aacaacaacc aatcagttta tcaatcagct cgggggggat 2040 
atttataaat tccatgctga acaactgggt gattttacct tagggattat gggaggatac 2100 
gcgaatgcaa aaggtaaaac gataaattac acgagcaaca aagctgccag aaacacactg 2160 
gatggttatt ctgtcggggt atacggtacg tggtatcaga atggggaaaa tgcaacaggg 2220 
ctctttgctg aaacttggat gcaatataac tggtttaatg catcagtgaa aggtgacgga 2280 
ctggaagaag aaaaatataa tctgaatggt ttaaccgctt ctgcaggtgg gggatataac 2340 
ctgaatgtgc acacatggac atcacctgaa ggaataacag gtgaattctg gttacagcct 2400 
catttgcagg ctgtctggat gggggttaca ccggatacac atcaggagga taacggaacg 2460 
gtggtgcagg gagcagggaa aaataatatt cagacaaaag caggtattcg tgcatcctgg 2520 
aaggtgaaaa gcaccctgga taaggatacc gggcggaggt tccgtccgta tatagaggca 2580 
aactggatcc ataacactca tgaatttggt gttaaaatga gtgatgacag ccagttgttg 2640 
tcaggtagcc gaaatcaggg agagataaag acaggtattg aaggggtgat tactcaaaac 2700 
ttgtcagtga atggcggagt cgcatatcag gcaggaggtc acgggagcaa tgccatctcc 2760 
ggagcactgg ggataaaata cagcttctga 2790 
<210> 2 
<211>929 
<212> PRT 
<213> artificial 
<220> 
<223> construct comprising autotransporter domain and NADH oxidase 
<400> 2 
Met Val Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
  1         5                  10              15 
Ala Tyr Ala His Gly Thr Pro Gln Asn Ile Thr Asp Leu Leu Glu Lys 
         20               25               30 
Val Thr Val Val Gly Cys Thr His Ala Gly Thr Phe Ala Ile Lys Gln 
      35              40               45 

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Ile Leu Ala Giu His Pro Asp Ala Gln Val Thr Val Tyr Glu Arg Asn 
    50              55               60 
Asp Val Ile Ser Phe Leu Ser Cys Gly Ile Ala Leu Tyr Leu Gly Gly 
65               70               75                80 
Lys Val Ala Asp Pro Gln Gly Leu Phe Tyr Ser Ser Pro Glu Glu Leu 
            85              90                95 
Gln Lys Leu Gly Ala Asn Val Gln Met Asn His Asn Val Leu Ala Ile 
        100               105              110 
Asp Pro Asp Gln Lys Thr Val Thr Val Glu Asp Leu Thr Ser His Ala 
      115              120              125 
Gln Thr Thr Glu Ser Tyr Asp Lys Leu Val Met Thr Ser Gly Ser Trp 
   130              135                140 
Pro Ile Val Pro Lys Ile Pro Gly Ile Asp Ser Asp Arg Val Lys Leu 
145            150                 155              160 
Cys Lys Asn Trp Ala His Ala Gln Ala Leu Ile Glu Asp Ala Lys Glu 
              165            170              175 
Ala Lys Arg Ile Thr Val Ile Gly Ala Gly Tyr Ile Gly Ala Glu Leu 
          180             185              190 
Ala Glu Ala Tyr Ser Thr Thr Gly His Asp Val Thr Leu Ile Asp Ala
      195              200               205 
Met Asp Arg Val Met Pro Lys Tyr Phe Asp Ala Asp Phe Thr Asp Val 
   210              215               220 
Ile Glu Gln Asp Tyr Arg Asp His Gly Val Gln Leu Ala Leu Ser Glu
225             230               235             240 
Thr Val Glu Ser Phe Thr Asp Ser Ala Thr Gly Leu Thr Ile Lys Thr 
             245               250               255 
Asp Lys Asn Ser Tyr Glu Thr Asp Leu Ala Ile Leu Cys Ile Gly Phe 
          260              265              270 
Arg Pro Asn Thr Asp Leu Leu Lys Gly Lys Val Asp Met Ala Pro Asn 
       275             280               285 
Gly Ala Ile Ile Thr Asp Asp Tyr Met Arg Ser Ser Asn Pro Asp Ile 
   290              295              300 
Phe Als Ala Gly Asp Ser Ala Ala Val His Tyr Asn Pro Thr His Gln 
305             310              315              320 

Page 3 

Asn Ala Tyr Ile Pro Leu Ala Thr Asn Ala Val Arg Gln Gly Ile Leu 
             325              330               335 
Val Gly Lys Asn Leu Val Lys Pro Thr Val Lys Tyr Met Gly Thr Gln
        340                345             350 
Ser Ser Ser Gly Leu Ala Leu Tyr Asp Arg Thr Ile Val Ser Thr Gly 
        355              360              365 
Leu Thr Leu Ala Ala Ala Lys Gln Gln Gly Val Asn Ala Glu Gln Val 
    370             375               380 
Ile Val Glu Asp Asn Tyr Arg Pro Glu Phe Met Pro Ser Thr Glu Pro 
385              390              395              400 
Val Leu Met Ser Leu Val Phe Asp Pro Asp Thr His Arg Ile Leu Gly 
             405              410               415 
Gly Ala Leu Met Ser Lys Tyr Asp Val Ser Gln Ser Ala Asn Thr Leu 
        420                425              430 
Ser Val Cys Ile Gln Asn Gu Asn Thr Ile Asp Asp Leu Ala Met Val 
       435             440              445 
Asp Met Leu Phe Gln Pro Asn Phe Asp Arg Pro Phe Asn Tyr Leu Asn
    450             455                460 
Ile Leu Ala Gln Ala Ala Gln Ala Lys Val Ala Gln Ser Val Asn Ala 
465              470             475               480 
Gly Thr Leu Asn Pro Thr Lys Glu Ser Ala Gly Asn Thr Leu Thr Val 
             485              490              495 
Ser Asn Tyr Thr Gly Thr Pro Gly Ser Val Ile  Ser Leu Gly Gly Val 
          500              505               510 
Leu Glu Gly Asp Asn Ser Leu Thr Asp Arg Leu Val Val Lys Gly Asn 
       515             520                525 
Thr Ser Gly Gln Ser Asp Ile Val Tyr Val Asn Glu Asp Gly Ser Gly 
   530               535              540 
Gly Gln Thr Arg Asp Gly Ile Asn Ile Ile Ser Val Glu Gly Asn Ser 
545               550             555               560 
Asp Ala Glu Phe Ser Leu Lys Asn Arg Val Val Ala Gly Ala Tyr Asp 
            565               570              575 
Tyr Thr Leu Gln Lys Gly Asn Glu Ser Gly Thr Asp Asn Lys Gly Trp 
         580               585               590 

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Tyr Leu Thr Ser His Leu Pro Thr Ser Asp Thr Arg Gln Tyr Arg Pro 
        595             600               605 
Glu Asn Gly Ser Tyr Ala Thr Asn Met Ala Leu Ala Asn Ser Leu Phe 
   610              615              620 
Leu Met Asp Leu Asn Glu Arg Lys Gln Phe Arg Ala Met Ser Asp Asn 
625              630               635             640 
Thr Gln Pro Glu Ser Ala Ser Val Trp Met Lys Ile Thr Gly Gly Ile 
             645              650                655 
Ser Ser Gly Lys Leu Asn Asp Gly Gln Asn Lys Thr Thr Thr Asn Gln 
           660              665             670 
Phe Ile Asn Gln Leu Gly Gly Asp Ile Tyr Lys Phe His Ala Glu Gin 
       675              680              685 
Leu Gly Asp Phe Thr Leu Gly Ile Met Gly Gly Tyr Ala Asn Ala Lys 
   690              695               700 
Gly Lys Thr Ile Asn Tyr Thr Ser Asn Lys Ala Ala Arg Asn Thr Leu 
705              710               715              720 
Asp Gly Tyr Ser Val Gly Val Tyr Gly Thr Trp Tyr Gln Asn Gly Glu 
             725             730               735 
Asn Ala Thr Gly Leu Phe Ala Glu Thr Trp Met Gln Tyr Asn Trp Phe 
         740               745              750 
Asn Ala Ser Val Lys Gly Asp Gly Leu Glu Glu Glu Lys Tyr Asn Leu 
       755              760             765 
Asn Gly Leu Thr Ala Ser Ala Gly Gly Gly Tyr Asn Leu Asn Val His 
   770              775               780 
Thr Trp Thr Ser Pro Glu Gly Ile Thr Gly Glu Phe Trp Leu Gln Pro 
785               790              795              800 
His Leu Gln Ala Val Trp Met Gly Val Thr Pro Asp Thr His Gln Glu 
             805              810              815 
Asp Asn Gly Thr Val Val Gln Gly Ala Gly Lys Asn Asn Ile Gln Thr 
          820             825             830 
Lys Ala Gly Ile Arg Ala Ser Trp Lys Val Lys Ser Thr Leu Asp Lys 
       835              840             845 
Asp Thr Gly Arg Arg Phe Arg Pro Tyr Ile Glu Ala Asn Trp Ile His 
   850              855               860 

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Asn Thr His Glu Phe Gly Val Lys Met Ser Asp Asp Ser Gln Leu Leu 
865              870              875              880 
Ser Gly Ser Arg Asn Gln Gly Glu Ile Lys Thr Gly Ile Gli Gly Val 
             885              890              895 
Ile Thr Gln Asn Leu Ser Val Asn Gly Gly Val Ala Tyr Gln Ala Gly 
           900              905             910 
Gly His Gly Ser Asn Ala Ile Ser Gly Ala Leu Gly Ile Lys Tyr Ser 
      915              920               925 

Phe 

<210> 3 
<211> 28 
<212> DNA 
<213> artificial
<220> 
<223> forward primer used to clone NADH oxidase 
<400> 3 
atctcgagaa agtcacagtt gttggttg 28 
<210> 4 
<211> 25 
<212> DNA 
<213> artificial
<220> 
<223> reverse primer used to clone NADH oxidase
<400> 4 
atggtaccag cgttaactga ttggg 25 

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