SEQUENCE LISTING

<110>  Pieris AG 

<120>  Binding proteins for hepcidin

<130>  PIE14047PCT

<140>  New PCT application
<141>

<150>  US 61/374,199
<151>  2010-08-16

<160>  35    

<170>  PatentIn version 3.5


<210>  1
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 


<400>  1

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Glu Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Phe Leu Ala Lys Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Thr Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         
                                 
        

<210>  2
<211>   178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 


<400>  13

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Thr Ala Gly Asn Ser Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Gln Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Arg Val Phe Phe Glu Gly Lys Lys Cys Arg Tyr Val Ile 
65                  70                  75                  80  
Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 
                85                  90                  95      
Ile Lys Ser Ala Pro Gly Gly Thr Ser Ile Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Leu Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


        
<210>  3
<211>  186
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 


<400>  3

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Val Ala Gly Asn Gly Leu Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Leu Lys Met His Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Arg Val Leu Phe Val Arg Lys Lys Cys Arg Tyr Tyr Ile 
65                  70                  75                  80  
Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Arg 
                85                  90                  95      
Ile Lys Ser Glu Pro Gly Arg Thr Ser Phe Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Met Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         

        

<210>  4
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 


<400>  4

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Val Ala Gly Asn Glu Met Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Leu Lys Met Leu Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Arg Val Met Phe Glu Tyr Lys Lys Cys Val Tyr Leu Ile 
65                  70                  75                  80  
Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Thr 
                85                  90                  95      
Ile Lys Ser Val Pro Gly Leu Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Arg Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


<210>  5
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 


<400>  5

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Ala Ala Gly Asn Ser Leu Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Arg Val Asn Phe Gly Gly Lys Lys Cys Ser Tyr Leu Ile 
65                  70                  75                  80  
Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ser 
                85                  90                  95      
Ile Lys Ser Arg Pro Gly Ala Thr Ser Val Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Leu Val Thr Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


<210>  6
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 


<400>  6

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Leu Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Arg Val Gln Phe Gly Glu Lys Lys Cys Gly Tyr Gly Ile 
65                  70                  75                  80  
Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ser 
                85                  90                  95      
Ile Lys Ser Val Pro Gly Gly Thr Ser Arg Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         

        
<210>  7
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  7

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Arg Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Gln Lys Met Phe Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Gly Val Asp Phe Arg Thr Lys Lys Cys Leu Tyr Ser Ile 
65                  70                  75                  80  
Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Val
                85                  90                  95      
Ile Lys Ser Gln Pro Gly Trp Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Thr Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


<210>  8
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  8

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Pro Leu Ala Glu Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 
                85                  90                  95      
Ile Lys Ser Gly Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Val Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


<210>  9
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  9

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Ser Leu Ala Lys Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Gly Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly S         


<210>  10
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  10

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Arg Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Phe Leu Ala Lys Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Pro Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly Phe         
      

<210>  11
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  11

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Phe Leu Ala Lys Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Gly Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly          


<210>  12
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  12

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Phe Leu Ala Glu Glu Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Lys Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly          


<210>  13
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  13

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Pro Leu Ala Glu Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe Thr Leu Gly Gly 
                85                  90                  95      
Ile Lys Ser Gly Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Val Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


<210>  14
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL 

<400>  14

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Glu Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Phe Leu Ala Lys Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Thr Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly         


<210>  15
<211>  243
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein of hNGAL with ABD domain and STREP tag 

<400>  15

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Met Lys Met Trp Ala Thr Ile Tyr Glu Leu Glu Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ile Val Met Phe Leu Ala Lys Lys Cys Glu Tyr Leu Phe 
65                  70                  75                  80  
Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Ser Pro Gly Arg Thr Ser Gly Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val Trp Gln 
        115                 120                 125             
Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly Ser Ala Gly Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Ly  
            180                 185                 190         
Val Leu Ala Asn Arg Glu Leu Asp Lys Tyr Gly Val Ser Asp Tyr Tyr 
        195                 200                 205             
Lys Asn Leu Ile Asn Asn Ala Lys Thr Val Glu Gly Val Lys Ala Leu 
    210                 215                 220                 
Ile Asp Glu Ile Leu Ala Ala Leu Pro Ser Ala Trp Ser His Pro Gln 
225                 230                 235                 240 
Phe Glu Lys          


<210>  16
<211>  88
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 36,  40, 41, 49, 52

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (68)..(69)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (70)..(70)
<223>  k is g or t

<400>  16

gaagtggtat gtggtaggtn nkgcagggaa tnnknnkctc agagaagaca aagacccgnn     60
kaagatgnnk gccaccatct atgagctg                                        88


<210>  17
<211>  79
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 68, 70, 72, 73, 77, 79, 81

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t
<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(37)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (47)..(48)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (53)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (55)..(55)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  k is g or t

<400>  17

caagagctac aatgtcaccn nkgtcnnktt tnnknnkaag aagtgtnnkt acnnkatcnn     60
kacttttgtt ccaggttcc                                                  79


<210>  18
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 96, 100, 103, 106

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  k is g or t

<400>  18

ggcgagttca cgctgggcnn kattaagagt nnkcctggan nkacgagtnn kctcgtccga     60
gtggtgag                                                              68

<210>  19
<211>  68
<212>  DNA
<213>  Artificial

<220>
<223>  NNK oligomer for positions 125, 127, 132, 134

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  k is g or t

<220>
<221>  misc_feature
<222>  (46)..(47)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  k is g or t

<400>  19

gctatggtgt tcttcaagnn kgttnnkcaa aacagggagn nkttcnnkat caccctctac     60
gggagaac                                                              68


<210>  20
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  20

ggtgacattg tagctcttgt cttctttcag ctcatagatg gtggc                     45

<210>  21
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  21

gcccagcgtg aactcgcctg gctgggaacc tggaacaaaa gt                        42

<210>  22
<211>  54
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  22

cttgaagaac accatagcat gctggttgta gttggtgctc accactcgga cgag           54

<210>  23
<211>  64
<212>  DNA
<213>  Artificial

<220>
<223>  PCR-Primer with fixed nucleotide sequences corresponding to the 
       non-coding strand

<400>  23

ggagaagcgg atgaagttct cctttagttc cgaagtcagc tccttggttc tcccgtagag     60
ggtg                                                                  64


<210>  24
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  5' flanking PCR-Oligo biotinylated

<400>  24
ccaggacaac caattccatg ggaagtggta tgtggtaggt                           40


<210>  25
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  3' flanking PCR-Oligo biotinylated

<400>  25

ttcagggagg cccagagatt tggagaagcg gatgaagttc                           40

<210>  26
<211>  4746
<212>  DNA
<213>  Artificial

<220>
<223>  Phage display vector phNGAL102 with CamR used as backbone for 
       NNK-Library

<400>  26

ccataacgct cggttgccgc cgggcgtttt ttattggcca gatgattaat tcctaatttt     60
tgttgacact ctatcattgg tagagttatt ttaccactcc ctatcagtga tagagaaaag    120
tgaaatgaat agttcgacaa aaatctagat aacgagggca aaaaatgaaa aagacagcta    180
tcgcgattgc agtggctctg gctggcttcg ctaccgtagc gcaggcccag gactccacct    240
cagacctgat cccagcccca cctctgagca aggtccctct gcagcagaac ttccaggaca    300
accaattcca tgggaagtgg tatgtggtag gtctcgcagg gaatgcaatt ctcagagaag    360
acaaagaccc gcaaaagatg tatgccacca tctatgagct gaaagaagac aagagctaca    420
atgtcacctc cgtcctgttt aggaaaaaga agtgtgacta ctggatcagg acttttgttc    480
caggttccca gccaggcgag ttcacgctgg gcaacattaa gagttaccct ggattaacga    540
gttacctcgt ccgagtggtg agcaccaact acaaccagca tgctatggtg ttcttcaaga    600
aagtttctca aaacagggag tacttcaaga tcaccctcta cgggagaacc aaggagctga    660
cttcggaact aaaggagaac ttcatccgct tctccaaatc tctgggcctc cctgaaaacc    720
acatcgtctt ccctgtccca atcgaccagt gtatcgacgg cagcgctggt ggggcctaga    780
ctgttgaaag ttgtttagca aaaccccata cagaaaattc atttactaac gtctggaaag    840
acgacaaaac tttagatcgt tacgctaact atgagggctg tctgtggaat gctacaggcg    900
ttgtagtttg tactggtgac gaaactcagt gttacggtac atgggttcct attgggcttg    960
ctatccctga aaatgagggt ggtggctctg agggtggcgg ttctgagggt ggcggttctg   1020
agggtggcgg tactaaacct cctgagtacg gtgatacacc tattccgggc tatacttata   1080
tcaaccctct cgacggcact tatccgcctg gtactgagca aaaccccgct aatcctaatc   1140
cttctcttga ggagtctcag cctcttaata ctttcatgtt tcagaataat aggttccgaa   1200
ataggcaggg ggcattaact gtttatacgg gcactgttac tcaaggcact gaccccgtta   1260
aaacttatta ccagtacact cctgtatcat caaaagccat gtatgacgct tactggaacg   1320
gtaaattcag agactgcgct ttccattctg gctttaatga ggatccattc gtttgtgaat   1380
atcaaggcca atcgtctgac ctgcctcaac ctcctgtcaa tgctggcggc ggctctggtg   1440
gtggttctgg tggcggctct gagggtggtg gctctgtggg tggcggttct gagggtggcg   1500
gctctgaggg aggcggttcc ggtggtggct ctggttccgg tgattttgat tatgaaaaga   1560
tggcaaacgc taataagggg gctatgaccg aaaatgccga tgaaaacgcg ctacagtctg   1620
acgctaaagg caaacttgat tctgtcgcta ctgattacgg tgctgctatc gatggtttca   1680
ttggtgacgt ttccggcctt gctaatggta atggtgctac tggtgatttt gctggctcta   1740
attcccaaat ggctcaagtc ggtgacggtg ataattcacc tttaatgaat aatttccgtc   1800
aatatttacc ttccctccct caatcggttg aatgtcgccc ttttgtcttt ggcgctggta   1860
aaccatatga attttctatt gattgtgaca aaataaactt attccgtggt gtctttgcgt   1920
ttcttttata tgttgccacc tttatgtatg tattttctac gtttgctaac atactgcgta   1980
ataaggagtc ttaataagct tgacctgtga agtgaaaaat ggcgcacatt gtgcgacatt   2040
ttttttgtct gccgtttacc gctactgcgt cacggatctc cacgcgccct gtagcggcgc   2100
attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct   2160
agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg   2220
tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga   2280
ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt   2340
ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg   2400
aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc   2460
ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat   2520
ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat   2580
aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa   2640
aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga   2700
acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga   2760
tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat   2820
tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg   2880
tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga   2940
aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata   3000
ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga   3060
gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt   3120
ggccaatatg gacaacttct tcgcccccgt tttcactatg ggcaaatatt atacgcaagg   3180
cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca   3240
tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta   3300
ataggaatta atgatgtctc gtttagataa aagtaaagtg attaacagcg cattagagct   3360
gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt   3420
agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc   3480
cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca   3540
agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg   3600
agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca   3660
attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc   3720
agtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga   3780
agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt   3840
atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg   3900
attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag cagcataacc tttttccgtg   3960
atggtaactt cactagttta aaaggatcta ggtgaagatc ctttttgata atctcatgac   4020
caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa   4080
aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc   4140
accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt   4200
aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg   4260
ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc   4320
agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt   4380
accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga   4440
gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct   4500
tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg   4560
cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca   4620
cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa   4680
cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgac   4740
ccgaca                                                              4746


<210>  27
<211>  4963
<212>  DNA
<213>  Artificial

<220>
<223>  Phage display vector phNGAL108 with AmpR used for Sloning Library

<400>  27

ccatcgaatg gccagatgat taattcctaa tttttgttga cactctatca ttgatagagt     60
tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct    120
agataacgag ggcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc tctggctggc    180
ttcgctaccg tagcgcaggc ccaggactcc acctcagacc tgatcccagc cccacctctg    240
agcaaggtcc ctctgcagca gaacttccag gacaaccaat tccatgggaa gtggtatgtg    300
gtaggtctcg cagggaatgc aattctcaga gaagacaaag acccgcaaaa gatgtatgcc    360
accatctatg agctgaaaga agacaagagc tacaatgtca cctccgtcct gtttaggaaa    420
aagaagtgtg actactggat caggactttt gttccaggtt cccagccagg cgagttcacg    480
ctgggcaaca ttaagagtta ccctggatta acgagttacc tcgtccgagt ggtgagcacc    540
aactacaacc agcatgctat ggtgttcttc aagaaagttt ctcaaaacag ggagtacttc    600
aagatcaccc tctacgggag aaccaaggag ctgacttcgg aactaaagga gaacttcatc    660
cgcttctcca aatctctggg cctccctgaa aaccacatcg tcttccctgt cccaatcgac    720
cagtgtatcg acggcagcgc ttggcgtcac ccgcagttcg gtggggccta gactgttgaa    780
agttgtttag caaaacccca tacagaaaat tcatttacta acgtctggaa agacgacaaa    840
actttagatc gttacgctaa ctatgagggc tgtctgtgga atgctacagg cgttgtagtt    900
tgtactggtg acgaaactca gtgttacggt acatgggttc ctattgggct tgctatccct    960
gaaaatgagg gtggtggctc tgagggtggc ggttctgagg gtggcggttc tgagggtggc   1020
ggtactaaac ctcctgagta cggtgataca cctattccgg gctatactta tatcaaccct   1080
ctcgacggca cttatccgcc tggtactgag caaaaccccg ctaatcctaa tccttctctt   1140
gaggagtctc agcctcttaa tactttcatg tttcagaata ataggttccg aaataggcag   1200
ggggcattaa ctgtttatac gggcactgtt actcaaggca ctgaccccgt taaaacttat   1260
taccagtaca ctcctgtatc atcaaaagcc atgtatgacg cttactggaa cggtaaattc   1320
agagactgcg ctttccattc tggctttaat gaggatccat tcgtttgtga atatcaaggc   1380
caatcgtctg acctgcctca acctcctgtc aatgctggcg gcggctctgg tggtggttct   1440
ggtggcggct ctgagggtgg tggctctgag ggtggcggtt ctgagggtgg cggctctgag   1500
ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg attatgaaaa gatggcaaac   1560
gctaataagg gggctatgac cgaaaatgcc gatgaaaacg cgctacagtc tgacgctaaa   1620
ggcaaacttg attctgtcgc tactgattac ggtgctgcta tcgatggttt cattggtgac   1680
gtttccggcc ttgctaatgg taatggtgct actggtgatt ttgctggctc taattcccaa   1740
atggctcaag tcggtgacgg tgataattca cctttaatga ataatttccg tcaatattta   1800
ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct ttggcgctgg taaaccatat   1860
gaattttcta ttgattgtga caaaataaac ttattccgtg gtgtctttgc gtttctttta   1920
tatgttgcca cctttatgta tgtattttct acgtttgcta acatactgcg taataaggag   1980
tcttaataag cttgacctgt gaagtgaaaa atggcgcaca ttgtgcgaca ttttttttgt   2040
ctgccgttta ccgctactgc gtcacggatc tccacgcgcc ctgtagcggc gcattaagcg   2100
cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg   2160
ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc   2220
taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa   2280
aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc   2340
ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac   2400
tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt   2460
ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc   2520
ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt   2580
tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat   2640
aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt   2700
ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg   2760
ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga   2820
tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc   2880
tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac   2940
actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg   3000
gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca   3060
acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg   3120
gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg   3180
acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg   3240
gcgaactact tactctagct tcccggcaac aattgataga ctggatggag gcggataaag   3300
ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg   3360
gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct   3420
cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac   3480
agatcgctga gataggtgcc tcactgatta agcattggta ggaattaatg atgtctcgtt   3540
tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag   3600
gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc   3660
atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc   3720
atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta   3780
aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac   3840
ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag   3900
gtttttcact agagaatgca ttatatgcac tcagcgcagt ggggcatttt actttaggtt   3960
gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg   4020
atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc   4080
cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg   4140
aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg gtaacttcac tagtttaaaa   4200
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt   4260
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt   4320
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt   4380
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga   4440
taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag   4500
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata   4560
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg   4620
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga   4680
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca   4740
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa   4800
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt   4860
tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac   4920
ggttcctggc cttttgctgg ccttttgctc acatgacccg aca                     4963

     
<210>  28
<211>  25
<212>  PRT
<213>  human

<220>
<223>  Hepcidin-25 

<400>  28

Asp Thr His Phe Pro Ile Cys Ile Phe Cys Cys Gly Cys Cys His Arg 
1               5                   10                  15      
Ser Lys Cys Gly Met Cys Cys Lys Thr
            20                  25               

     
<210>  29
<211>  25
<212>  PRT
<213>  mouse

<220>
<223>  Hepcidin-25 

<400>  29

Asp Thr Asn Phe Pro Ile Cys Ile Phe Cys Cys Lys Cys Cys Asn Asn 
1               5                   10                  15      
Ser Gln Cys Gly Ile Cys Cys Lys Thr
            20                  25               


     
<210>  30
<211>  25
<212>  PRT
<213>  rat

<220>
<223>  Hepcidin-25  

<400>  30

Asp Thr Asn Phe Pro Ile Cys Leu Phe Cys Cys Lys Cys Cys Lys Asn 
1               5                   10                  15      
Ser Ser Cys Gly Leu Cys Cys Ile Thr
            20                  25               
        

<210>  31
<211>  3745
<212>  DNA
<213>  Artificial

<220>
<223>  Expression vector phNGAL98 with AmpR encoding wild type Lcn2 
       with the C-terminal Strep-tagII

<400>  31

ccatcgaatg gccagatgat taattcctaa tttttgttga cactctatca ttgatagagt     60
tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct    120
agataacgag ggcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc tctggctggc    180
ttcgctaccg tagcgcaggc ccaggactcc acctcagacc tgatcccagc cccacctctg    240
agcaaggtcc ctctgcagca gaacttccag gacaaccaat tccatgggaa gtggtatgtg    300
gtaggtctcg cagggaatgc aattctcaga gaagacaaag acccgcaaaa gatgtatgcc    360
accatctatg agctgaaaga agacaagagc tacaatgtca cctccgtcct gtttaggaaa    420
aagaagtgtg actactggat caggactttt gttccaggtt cccagccagg cgagttcacg    480
ctgggcaaca ttaagagtta ccctggatta acgagttacc tcgtccgagt ggtgagcacc    540
aactacaacc agcatgctat ggtgttcttc aagaaagttt ctcaaaacag ggagtacttc    600
aagatcaccc tctacgggag aaccaaggag ctgacttcgg aactaaagga gaacttcatc    660
cgcttctcca aatctctggg cctccctgaa aaccacatcg tcttccctgt cccaatcgac    720
cagtgtatcg acggcagcgc ttggtctcac ccgcagttcg aaaaataata agcttgacct    780
gtgaagtgaa aaatggcgca cattgtgcga catttttttt gtctgccgtt taccgctact    840
gcgtcacgga tctccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta    900
cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc    960
cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt   1020
tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg   1080
gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca   1140
cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct   1200
attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga   1260
tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcaggtggca   1320
cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata   1380
tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga   1440
gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc   1500
ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg   1560
cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc   1620
ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat   1680
cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact   1740
tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat   1800
tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga   1860
tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc   1920
ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga   1980
tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag   2040
cttcccggca acaattgata gactggatgg aggcggataa agttgcagga ccacttctgc   2100
gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct   2160
ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct   2220
acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg   2280
cctcactgat taagcattgg taggaattaa tgatgtctcg tttagataaa agtaaagtga   2340
ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac   2400
tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg   2460
ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt   2520
tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt   2580
tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt   2640
atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg   2700
cattatatgc actcagcgca gtggggcatt ttactttagg ttgcgtattg gaagatcaag   2760
agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat   2820
tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc   2880
ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaaaagc   2940
agcataacct ttttccgtga tggtaacttc actagtttaa aaggatctag gtgaagatcc   3000
tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag   3060
accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct   3120
gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac   3180
caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc   3240
tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg   3300
ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt   3360
tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt   3420
gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc   3480
tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca   3540
gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata   3600
gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg   3660
ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct   3720
ggccttttgc tcacatgacc cgaca                                         3745


<210>  32
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  32

cccaggactc cacctcagac c                                               21

<210>  33
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  33

actgcgggtg ggaccaagcg ctgccgt                                         27 

<210>  34
<211>  197
<212>  PRT
<213>  Artificial

<220>
<223>  hNGAL with T7 tag encoded by phNGAL 101

<400>  34

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  
Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 
                85                  90                  95      
Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Thr Val Ser Gln 
        115                 120                 125             
Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly Ser Ala Trp Ser His Pro Gln Phe Met Ala Ser Met Thr Gly 
            180                 185                 190         
Gly Gln Gln Met Gly 
        195                              


<210>  35
<211>  178
<212>  PRT
<213>  Artificial

<220>
<223>  wild type hNGAL

<400>  35

Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 
65                  70                  75                  80  
Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      
Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 
        115                 120                 125             
Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp Gly 

1


