
                                SEQUENCE LISTING

<110> Tessie McNEELY
      Hongxia FAN
      Mark Andrew MILLER

<120> STAPHYLOCOCCUS AUREUS SURFACE PROTEIN
  SA1789 AND PROTECTIVE VACCINE BASED THEREON
  

<130> MRL-IFD-00030

<150> 61/363,893          
<151> 2010-07-13  

<150> 61/366,360          
<151> 2010-07-21  

<160> 7

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 489
<212> DNA
<213> Staphylococcus aureus

<400> 1
atggattgga ttttaccaat tgctggaatt atcgctgcga ttgcattctt aattttatgt 60
atcggtatcg tagctgtatt aaattctgtt aagaaaaact tagattatgt tgcaaaaaca 120
cttgacggtg tagaaggtca agttcaaggt attactcgtg aaacaacaga tttacttcat 180
aaagtaaacc gtttaactga ggatatccaa ggtaaagtag atcgtttaaa ctcagttgta 240
gatgctgtta aaggtatcgg tgactcagta caaacgttaa acagctctgt agatcgtgta 300
acaaattcaa ttacacataa tatttctcaa aatgaagata aaatctcaca agttgttcaa 360
tggtcaaatg ttgcaatgga aattgcagac aaatggcaaa atagacacta ccgtcgtgga 420
agtgcaaatt acaaagctaa taatgtagca actgatgcaa atcatagcta tacttctaga 480
gtagataaa                                                         489

<210> 2
<211> 138
<212> PRT
<213> Staphylococcus aureus

<400> 2
Val Leu Asn Ser Val Lys Lys Asn Leu Asp Tyr Val Ala Lys Thr Leu
 1               5                  10                  15      
Asp Gly Val Glu Gly Gln Val Gln Gly Ile Thr Arg Glu Thr Thr Asp
            20                  25                  30          
Leu Leu His Lys Val Asn Arg Leu Thr Glu Asp Ile Gln Gly Lys Val
        35                  40                  45              
Asp Arg Leu Asn Ser Val Val Asp Ala Val Lys Gly Ile Gly Asp Ser
    50                  55                  60                  
Val Gln Thr Leu Asn Ser Ser Val Asp Arg Val Thr Asn Ser Ile Thr
65                  70                  75                  80  
His Asn Ile Ser Gln Asn Glu Asp Lys Ile Ser Gln Val Val Gln Trp
                85                  90                  95      
Ser Asn Val Ala Met Glu Ile Ala Asp Lys Trp Gln Asn Arg His Tyr
            100                 105                 110         
Arg Arg Gly Ser Ala Asn Tyr Lys Ala Asn Asn Val Ala Thr Asp Ala
        115                 120                 125             
Asn His Ser Tyr Thr Ser Arg Val Asp Lys
    130                 135             


<210> 3
<211> 163
<212> PRT
<213> Artificial Sequence

<220> 
<223> SA1789 protein with amino terminal His-tag and
      three additional amino acids at carboxy terminus

<400> 3
Met Gly His His His His His His His His His His Ser Ser Gly His
 1               5                  10                  15      
Ile Glu Gly Arg His Met Val Leu Asn Ser Val Lys Lys Asn Leu Asp
            20                  25                  30          
Tyr Val Ala Lys Thr Leu Asp Gly Val Glu Gly Gln Val Gln Gly Ile
        35                  40                  45              
Thr Arg Glu Thr Thr Asp Leu Leu His Lys Val Asn Arg Leu Thr Glu
    50                  55                  60                  
Asp Ile Gln Gly Lys Val Asp Arg Leu Asn Ser Val Val Asp Ala Val
65                  70                  75                  80  
Lys Gly Ile Gly Asp Ser Val Gln Thr Leu Asn Ser Ser Val Asp Arg
                85                  90                  95      
Val Thr Asn Ser Ile Thr His Asn Ile Ser Gln Asn Glu Asp Lys Ile
            100                 105                 110         
Ser Gln Val Val Gln Trp Ser Asn Val Ala Met Glu Ile Ala Asp Lys
        115                 120                 125             
Trp Gln Asn Arg His Tyr Arg Arg Gly Ser Ala Asn Tyr Lys Ala Asn
    130                 135                 140                 
Asn Val Ala Thr Asp Ala Asn His Ser Tyr Thr Ser Arg Val Asp Lys
145                 150                 155                 160 
Ala Glu Gln
            


<210> 4
<211> 163
<212> PRT
<213> Staphylococcus aureus

<220> 

<400> 4
Met Asp Trp Ile Leu Pro Ile Ala Gly Ile Ile Ala Ala Ile Ala Phe
 1               5                  10                  15      
Leu Ile Leu Cys Ile Gly Ile Val Ala Val Leu Asn Ser Val Lys Lys
            20                  25                  30          
Asn Leu Asp Tyr Val Ala Lys Thr Leu Asp Gly Val Glu Gly Gln Val
        35                  40                  45              
Gln Gly Ile Thr Arg Glu Thr Thr Asp Leu Leu His Lys Val Asn Arg
    50                  55                  60                  
Leu Thr Glu Asp Ile Gln Gly Lys Val Asp Arg Leu Asn Ser Val Val
65                  70                  75                  80  
Asp Ala Val Lys Gly Ile Gly Asp Ser Val Gln Thr Leu Asn Ser Ser
                85                  90                  95      
Val Asp Arg Val Thr Asn Ser Ile Thr His Asn Ile Ser Gln Asn Glu
            100                 105                 110         
Asp Lys Ile Ser Gln Val Val Gln Trp Ser Asn Val Ala Met Glu Ile
        115                 120                 125             
Ala Asp Lys Trp Gln Asn Arg His Tyr Arg Arg Gly Ser Ala Asn Tyr
    130                 135                 140                 
Lys Ala Asn Asn Val Ala Thr Asp Ala Asn His Ser Tyr Thr Ser Arg
145                 150                 155                 160 
Val Asp Lys
            


<210> 5
<211> 645
<212> PRT
<213> Staphylococcus aureus

<400> 5
Met Asn Lys Gln Gln Lys Glu Phe Lys Ser Phe Tyr Ser Ile Arg Lys
 1               5                  10                  15      
Ser Ser Leu Gly Val Ala Ser Val Ala Ile Ser Thr Leu Leu Leu Leu
            20                  25                  30          
Met Ser Asn Gly Glu Ala Gln Ala Ala Ala Glu Glu Thr Gly Gly Thr
        35                  40                  45              
Asn Thr Glu Ala Gln Pro Lys Thr Glu Ala Val Ala Ser Pro Thr Thr
    50                  55                  60                  
Thr Ser Glu Lys Ala Pro Glu Thr Lys Pro Val Ala Asn Ala Val Ser
65                  70                  75                  80  
Val Ser Asn Lys Glu Val Glu Ala Pro Thr Ser Glu Thr Lys Glu Ala
                85                  90                  95      
Lys Glu Val Lys Glu Val Lys Ala Pro Lys Glu Thr Lys Glu Val Lys
            100                 105                 110         
Pro Ala Ala Lys Ala Thr Asn Asn Thr Tyr Pro Ile Leu Asn Gln Glu
        115                 120                 125             
Leu Arg Glu Ala Ile Lys Asn Pro Ala Ile Lys Asp Lys Asp His Ser
    130                 135                 140                 
Ala Pro Asn Ser Arg Pro Ile Asp Phe Glu Met Lys Lys Lys Asp Gly
145                 150                 155                 160 
Thr Gln Gln Phe Tyr His Tyr Ala Ser Ser Val Lys Pro Ala Arg Val
                165                 170                 175     
Ile Phe Thr Asp Ser Lys Pro Glu Ile Glu Leu Gly Leu Gln Ser Gly
            180                 185                 190         
Gln Phe Trp Arg Lys Phe Glu Val Tyr Glu Gly Asp Lys Lys Leu Pro
        195                 200                 205             
Ile Lys Leu Val Ser Tyr Asp Thr Val Lys Asp Tyr Ala Tyr Ile Arg
    210                 215                 220                 
Phe Ser Val Ser Asn Gly Thr Lys Ala Val Lys Ile Val Ser Ser Thr
225                 230                 235                 240 
His Phe Asn Asn Lys Glu Glu Lys Tyr Asp Tyr Thr Leu Met Glu Phe
                245                 250                 255     
Ala Gln Pro Ile Tyr Asn Ser Ala Asp Lys Phe Lys Thr Glu Glu Asp
            260                 265                 270         
Tyr Lys Ala Glu Lys Leu Leu Ala Pro Tyr Lys Lys Ala Lys Thr Leu
        275                 280                 285             
Glu Arg Gln Val Tyr Glu Leu Asn Lys Ile Gln Asp Lys Leu Pro Glu
    290                 295                 300                 
Lys Leu Lys Ala Glu Tyr Lys Lys Lys Leu Glu Asp Thr Lys Lys Ala
305                 310                 315                 320 
Leu Asp Glu Gln Val Lys Ser Ala Ile Thr Glu Phe Gln Asn Val Gln
                325                 330                 335     
Pro Thr Asn Glu Lys Met Thr Asp Leu Gln Asp Thr Lys Tyr Val Val
            340                 345                 350         
Tyr Glu Ser Val Glu Asn Asn Glu Ser Met Met Asp Thr Phe Val Lys
        355                 360                 365             
His Pro Ile Lys Thr Gly Met Leu Asn Gly Lys Lys Tyr Met Val Met
    370                 375                 380                 
Glu Thr Thr Asn Asp Asp Tyr Trp Lys Asp Phe Met Val Glu Gly Gln
385                 390                 395                 400 
Arg Val Arg Thr Ile Ser Lys Asp Ala Lys Asn Asn Thr Arg Thr Ile
                405                 410                 415     
Ile Phe Pro Tyr Val Glu Gly Lys Thr Leu Tyr Asp Ala Ile Val Lys
            420                 425                 430         
Val His Val Lys Thr Ile Asp Tyr Asp Gly Gln Tyr His Val Arg Ile
        435                 440                 445             
Val Asp Lys Glu Ala Phe Thr Lys Ala Asn Thr Asp Lys Ser Asn Lys
    450                 455                 460                 
Lys Glu Gln Gln Asp Asn Ser Ala Lys Lys Glu Ala Thr Pro Ala Thr
465                 470                 475                 480 
Pro Ser Lys Pro Thr Pro Ser Pro Val Glu Lys Glu Ser Gln Lys Gln
                485                 490                 495     
Asp Ser Gln Lys Asp Asp Asn Lys Gln Leu Pro Ser Val Glu Lys Glu
            500                 505                 510         
Asn Asp Ala Ser Ser Glu Ser Gly Lys Asp Lys Thr Pro Ala Thr Lys
        515                 520                 525             
Pro Thr Lys Gly Glu Val Glu Ser Ser Ser Thr Thr Pro Thr Lys Val
    530                 535                 540                 
Val Ser Thr Thr Gln Asn Val Ala Lys Pro Thr Thr Ala Ser Ser Lys
545                 550                 555                 560 
Thr Thr Lys Asp Val Val Gln Thr Ser Ala Gly Ser Ser Glu Ala Lys
                565                 570                 575     
Asp Ser Ala Pro Leu Gln Lys Ala Asn Ile Lys Asn Thr Asn Asp Gly
            580                 585                 590         
His Thr Gln Ser Gln Asn Asn Lys Asn Thr Gln Glu Asn Lys Ala Lys
        595                 600                 605             
Ser Leu Pro Gln Thr Gly Glu Glu Ser Asn Lys Asp Met Thr Leu Pro
    610                 615                 620                 
Leu Met Ala Leu Leu Ala Leu Ser Ser Ile Val Ala Phe Val Leu Pro
625                 630                 635                 640 
Arg Lys Arg Lys Asn
                645 


<210> 6
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> SA1789 PCR primer, forward

<400> 6
gggaattcca tatggtatta aattctgtta agaaaaactt ag                    42

<210> 7
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> SA1789 PCR primer, reverse

<400> 7
gggctcagct ttatctactc tagaagtata gcta                             34
