						SEQUENCE LISTING

<110>  NOTARANGELO, LUIGI
       PESSACH, ITAI
       PAQUES, FREDERIC

<120>  METHOD FOR INCREASING DOUBLE-STRAND BREAK-INDUCED GENE TARGETING

<130>  364916WO59

<150>  US 61/370,010
<150>  2010-08-02

<160>  7   

<170>  PatentIn version 3.5


<210>  1
<211>  163
<212>  PRT
<213>  Chlamydomonas reinhardtii

<400>  1

Met Asn Thr Lys Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly Phe 
1               5                   10                  15      


Val Asp Gly Asp Gly Ser Ile Ile Ala Gln Ile Lys Pro Asn Gln Ser 
            20                  25                  30          


Tyr Lys Phe Lys His Gln Leu Ser Leu Thr Phe Gln Val Thr Gln Lys 
        35                  40                  45              


Thr Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly Val 
    50                  55                  60                  


Gly Tyr Val Arg Asp Arg Gly Ser Val Ser Asp Tyr Ile Leu Ser Glu 
65                  70                  75                  80  


Ile Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys 
                85                  90                  95      


Leu Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln Leu 
            100                 105                 110         


Pro Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp 
        115                 120                 125             


Val Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr 
    130                 135                 140                 


Ser Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Glu Lys Lys Lys 
145                 150                 155                 160 


Ser Ser Pro 


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C1221 target

<400>  2
caaaacgtcg tacgacgttt tg                                              22


<210>  3
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  I-CreI monomer


<220>
<221>  MISC_FEATURE
<222>  (76)..(76)
<223>  allelic variantion: Asp or Asn

<400>  3

Met Ala Asn Thr Lys Tyr Asn Lys Glu Phe Leu Leu Tyr Leu Ala Gly 
1               5                   10                  15      


Phe Val Asp Gly Asp Gly Ser Ile Ile Ala Gln Ile Lys Pro Asn Gln 
            20                  25                  30          


Ser Tyr Lys Phe Lys His Gln Leu Ser Leu Thr Phe Gln Val Thr Gln 
        35                  40                  45              


Lys Thr Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly 
    50                  55                  60                  


Val Gly Tyr Val Arg Asp Arg Gly Ser Val Ser Asp Tyr Ile Leu Ser 
65                  70                  75                  80  


Glu Ile Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu 
                85                  90                  95      


Lys Leu Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln 
            100                 105                 110         


Leu Pro Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr 
        115                 120                 125             


Trp Val Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr 
    130                 135                 140                 


Thr Ser Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Glu Lys Lys 
145                 150                 155                 160 


Lys Ser Ser Pro Ala Ala Asp 
                165         

<210>  4
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RAG1 forward PCR primer

<400>  4
gcaagaggca aagcgatc                                                   18


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RAG1 reverse PCR primer

<400>  5
atgatgatcg ccatactg                                                   18


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RAG1 reverse real time PCR primer

<400>  6
tcacaggatg gtgtgtggg                                                  19


<210>  7
<211>  354
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  SC-OH-RAG-G19S-CLS

<400>  7

Met Ala Asn Thr Lys Tyr Asn Glu Glu Phe Leu Leu Tyr Leu Ala Gly 
1               5                   10                  15      


Phe Val Asp Gly Asp Gly Ser Ile Ile Ala Gln Ile Asn Pro Asn Gln 
            20                  25                  30          


Ser Ser Lys Phe Lys His Arg Leu Arg Leu Thr Phe Tyr Val Thr Gln 
        35                  40                  45              


Lys Thr Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Glu Ile Gly 
    50                  55                  60                  


Val Gly Tyr Val Arg Asp Ser Gly Ser Val Ser Gln Tyr Val Leu Ser 
65                  70                  75                  80  


Glu Ile Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu 
                85                  90                  95      


Glu Leu Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln 
            100                 105                 110         


Leu Pro Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr 
        115                 120                 125             


Trp Val Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr 
    130                 135                 140                 


Thr Ser Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Gly Lys Lys 
145                 150                 155                 160 


Lys Ser Ser Pro Ala Ala Gly Gly Ser Asp Lys Tyr Asn Gln Ala Leu 
                165                 170                 175     


Ser Lys Tyr Asn Gln Ala Leu Ser Lys Tyr Asn Gln Ala Leu Ser Gly 
            180                 185                 190         


Gly Gly Gly Ser Asn Lys Lys Phe Leu Leu Tyr Leu Ala Gly Phe Val 
        195                 200                 205             


Asp Ser Asp Gly Ser Ile Ile Ala Gln Ile Lys Pro Arg Gln Ser Asn 
    210                 215                 220                 


Lys Phe Lys His Gln Leu Ser Leu Thr Phe Ala Val Thr Gln Lys Thr 
225                 230                 235                 240 


Gln Arg Arg Trp Phe Leu Asp Lys Leu Val Asp Arg Ile Gly Val Gly 
                245                 250                 255     


Tyr Val Tyr Asp Ser Gly Ser Val Ser Asp Tyr Arg Leu Ser Glu Ile 
            260                 265                 270         


Lys Pro Leu His Asn Phe Leu Thr Gln Leu Gln Pro Phe Leu Lys Leu 
        275                 280                 285             


Lys Gln Lys Gln Ala Asn Leu Val Leu Lys Ile Ile Glu Gln Leu Pro 
    290                 295                 300                 


Ser Ala Lys Glu Ser Pro Asp Lys Phe Leu Glu Val Cys Thr Trp Val 
305                 310                 315                 320 


Asp Gln Ile Ala Ala Leu Asn Asp Ser Lys Thr Arg Lys Thr Thr Ser 
                325                 330                 335     


Glu Thr Val Arg Ala Val Leu Asp Ser Leu Ser Glu Lys Lys Lys Ser 
            340                 345                 350         


Ser Pro


