                         SEQUENCE LISTING

<110>  Ludwig-Maximilians-Universitt Mnchen
 
<120>  Identification of T cell target antigens

<130>  S2021 PCT

<150>  EP 10 00 8233.8
<151>  2010-08-06

<160>  112   

<170>  PatentIn version 3.3

<210>  1
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp1"

<220>  
<221>  variation
<222>  2
<223>  /replace="g"

<220>  
<221>  variation
<222>  3
<223>  /replace="t"

<400>  1
tcctttgtct cctgggagca                                                 20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp2"

<400>  2
cctgaagtcg cccagactcc                                                 20


<210>  3
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp3"

<220>  
<221>  variation
<222>  6
<223>  /replace="g"

<220>  
<221>  variation
<222>  19
<223>  /replace="t"

<400>  3
gtcatccaga acccaagaca cc                                              22


<210>  4
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp4"

<220>  
<221>  variation
<222>  3
<223>  /replace="t"

<220>  
<221>  variation
<222>  11
<223>  /replace="c"

<220>  
<221>  variation
<222>  14
<223>  /replace="c"

<400>  4
ggatatctgt aagagtggaa cctc                                            24


<210>  5
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp5

<220>  
<221>  variation
<222>  21
<223>  /replace="c"

<400>  5
atgtactggt atcgacaaga tc                                              22


<210>  6
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp6"

<400>  6
cactgtggaa ggaacatcaa acc                                             23


<210>  7
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp7"

<220>  
<221>  variation
<222>  11
<223>  /replace="g"

<400>  7
tctccactct caagatccag c                                               21


<210>  8
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp8"

<220>  
<221>  variation
<222>  4
<223>  /replace="g"

<220>  
<221>  variation
<222>  10
<223>  /replace="g"

<220>  
<221>  variation
<222>  13
<223>  /replace="t"

<400>  8
cagaatgtaa atctcaggtg tgatcc                                          26


<210>  9
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp9"

<220>  
<221>  variation
<222>  7
<223>  /replace="t"

<220>  
<221>  variation
<222>  12
<223>  /replace="g"

<220>  
<221>  variation
<222>  14
<223>  /replace="t"

<400>  9
ccagacacca aaacacctgg tca                                             23


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer C alpha out"

<400>  10
gcagacagac ttgtcactgg                                                 20


<210>  11
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer C beta out"

<400>  11
tggtcgggga agaagcctgt g                                               21


<210>  12
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer C alpha in"

<400>  12
agtctctcag ctggtacacg                                                 20


<210>  13
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer UP"

<400>  13
acagcacgac ttccaagact ca                                              22


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer C beta in"

<400>  14
tctgatggct caaacacagc                                                 20


<210>  15
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-1(14)-for-out"

<220>  
<221>  variation
<222>  3
<223>  /replace="g"

<400>  15
agcagcctca ctggagttg                                                  19


<210>  16
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-1(235)-for-out"

<400>  16
ctgaggtgca actactcatc                                                 20


<210>  17
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-2-for-out"
<220>  
<221>  variation
<222>  3
<223>  /replace="g"

<400>  17
caatgttcca gagggagcc                                                  19


<210>  18
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-3,25-for-out"

<220>  
<221>  variation
<222>  4
<223>  /replace="g"

<220>  
<221>  variation
<222>  8
<223>  /replace="t"

<220>  
<221>  variation
<222>  10
<223>  /replace="t"

<400>  18
gaaaatgcca ccatgaactg c                                               21


<210>  19
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-4,20-for-out"

<220>  
<221>  variation
<222>  1
<223>  /replace="t"

<400>  19
atgctaagac cacccagcc                                                  19


<210>  20
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-5-for-out"

<400>  20
agatagaaca gaattccgag g                                               21


<210>  21
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-6,14-for-out"

<220>  
<221>  variation
<222>  1
<223>  /replace="g"

<220>  
<221>  variation
<222>  2
<223>  /replace="t"

<400>  21
actgcacata tgacaccagt g                                               21


<210>  22
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-7-for-out"

<400>  22
cacgtaccag acatctggg                                                  19


<210>  23
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-8,21-for-out"

<220>  
<221>  variation
<222>  7
<223>  /replace="t"

<220>  
<221>  variation
<222>  16
<223>  /replace="g"

<400>  23
cctgagcgtc caggaagg                                                   18


<210>  24
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-9-for-out"

<400>  24
gtgcaactat tcctattctg g                                               21


<210>  25
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-10,24-for-out"

<220>  
<221>  variation
<222>  2
<223>  /replace="g"

<220>  
<221>  variation
<222>  13
<223>  /replace="t"

<400>  25
actggagcag agccctcag                                                  19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-11-for-out"

<400>  26
tcttcagagg gagctgtgg                                                  19


<210>  27
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-12-for-out"

<400>  27
ggtggagaag gaggatgtg                                                  19


<210>  28
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-13,19,26-for-out"

<220>  
<221>  variation
<222>  1
<223>  /replace="g"

<220>  
<221>  variation
<222>  4
<223>  /replace="g"

<400>  28
caactggagc agagtcctc                                                  19


<210>  29
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-15-for-out"

<400>  29
cctgagtgtc cgagaggg                                                   18


<210>  30
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-16-for-out"

<400>  30
atgcacctat tcagtctctg g                                               21


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-17-for-out"

<400>  31
tgatagtcca gaaaggaggg                                                 20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-18-for-out"

<400>  32
gtcactgcat gttcaggagg                                                 20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-22,31-for-out"

<220>  
<221>  variation
<222>  5
<223>  /replace="t"

<400>  33
ccctaccctt ttctggtatg                                                 20


<210>  34
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-23,30-for-out"

<220>  
<221>  variation
<222>  5
<223>  /replace="g"

<220>  
<221>  variation
<222>  8
<223>  /replace="t"

<400>  34
ggcaagaccc tgggaaagg                                                  19


<210>  35
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-27-for-out"

<400>  35
ctgttcctga gcatgcagg                                                  19


<210>  36
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-28-for-out"

<400>  36
agacaaggtg gtacaaagcc                                                 20


<210>  37
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-29-for-out"

<400>  37
caaccagtgc agagtcctc                                                  19


<210>  38
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-32-for-out"

<400>  38
gcatgtacaa gaaggagagg                                                 20


<210>  39
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp1+"

<220>  
<221>  variation
<222>  24
<223>  /replace="t"

<400>  39
acagcacgac ttccaagact cacctttgtc tcctgggagc a                         41


<210>  40
<211>  42
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp2+"

<400>  40
acagcacgac ttccaagact cacctgatgt cgcccagact cc                        42


<210>  41
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp3+"

<220>  
<221>  variation
<222>  28
<223>  /replace="g"

<220>  
<221>  variation
<222>  41
<223>  /replace="t"

<400>  41
acagcacgac ttccaagact cagtcatcca gaacccaaga cacc                      44


<210>  42
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp4+"

<220>  
<221>  variation
<222>  25
<223>  /replace="t"

<220>  
<221>  variation
<222>  33
<223>  /replace="c"

<220>  
<221>  variation
<222>  36
<223>  /replace="c"

<400>  42
acagcacgac ttccaagact caggatatct gtaagagtgg aacctc                    46


<210>  43
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp5+"

<220>  
<221>  variation
<222>  43
<223>  /replace="t"

<400>  43
acagcacgac ttccaagact caatgtactg gtatcgacaa gacc                      44


<210>  44
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp6+"

<400>  44
acagcacgac ttccaagact cacactgtgg aaggaacatc aaacc                     45


<210>  45
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp7+"

<220>  
<221>  variation
<222>  33
<223>  /replace="g"

<400>  45
acagcacgac ttccaagact catctccact ctcaagatcc agc                       43


<210>  46
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp8+"

<220>  
<221>  variation
<222>  26
<223>  /replace="g"

<220>  
<221>  variation
<222>  32
<223>  /replace="g"

<220>  
<221>  variation
<222>  35
<223>  /replace="t"

<400>  46
acagcacgac ttccaagact cacagaatgt aaatctcagg tgtgatcc                  48


<210>  47
<211>  45
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Vp9+"

<220>  
<221>  variation
<222>  29
<223>  /replace="t"

<220>  
<221>  variation
<222>  34
<223>  /replace="g"

<220>  
<221>  variation
<222>  36
<223>  /replace="t"

<400>  47
acagcacgac ttccaagact catcagacac caaaacacct ggtca                     45


<210>  48
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-4/1-for-in"

<400>  48
acagaagaca gaaagtccag c                                               21


<210>  49
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-4/2-for-in"

<400>  49
gtccagtacc ttgatcctgc                                                 20


<210>  50
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-6-for-in"

<400>  50
gcaaaatgca acagaaggtc g                                               21


<210>  51
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-8/1-for-in"

<400>  51
cagtgcctca aactacttcc                                                 20


<210>  52
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-8/2-for-in"

<400>  52
gcctcagact acttcatttg g                                               21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-14-for-in"

<400>  53
acagaatgca acggagaatc g                                               21


<210>  54
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-24-for-in"

<400>  54
ccttcagcaa cttaaggtgg                                                 20


<210>  55
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-28-for-in"

<400>  55
tctctggttg tccacgagg                                                  19


<210>  56
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-2/1-for-in"

<400>  56
tggaaggttt acagcacagc                                                 20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-2/2-for-in"

<400>  57
tggaaggttt acagcacagg                                                 20


<210>  58
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-5-for-in"

<400>  58
cagcatactt acagtggtac c                                               21


<210>  59
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-10-for-in"

<400>  59
tcactgtgta ctgcaactcc                                                 20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-12-for-in"

<400>  60
tacaagcaac caccaagtgg                                                 20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-22-for-in"

<400>  61
aggctgatga caagggaagc                                                 20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-31-for-in"

<400>  62
gtggaatacc ccagcaaacc                                                 20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-7-for-in"

<400>  63
ctccagatga aagactctgc                                                 20


<210>  64
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-13-for-in"

<400>  64
ttaagcgcca cgactgtcg                                                  19


<210>  65
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-17-for-in"

<400>  65
ctgtgcttat gagaacactg c                                               21


<210>  66
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-18-for-in"

<400>  66
ccttacactg gtacagatgg                                                 20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-21-for-in"

<400>  67
tgctgaaggt cctacattcc                                                 20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-23-for-in"

<400>  68
gtggaagact taatgcctcg                                                 20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-32-for-in"

<400>  69
tcaccacgta ctgcaattcc                                                 20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-3-for-in"

<400>  70
ttcaggtaga ggccttgtcc                                                 20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-11-for-in"

<400>  71
agggacgata caacatgacc                                                 20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-15-for-in"

<400>  72
cctccaccta cttatactgg                                                 20


<210>  73
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-19-for-in"

<400>  73
cctgcacatc acagcctcc                                                  19


<210>  74
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-25-for-in"

<400>  74
agactgactg ctcagtttgg                                                 20


<210>  75
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-26-for-in"

<400>  75
cctgcatatc acagcctcc                                                  19


<210>  76
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-29-for-in"

<400>  76
actgcagttc ctccaaggc                                                  19


<210>  77
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-1/235-for-in"

<400>  77
aaggcatcaa cggttttgag g                                               21


<210>  78
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-1/14-for-in"

<400>  78
ctgaggaaac cctctgtgc                                                  19


<210>  79
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-9-for-in"

<400>  79
atctttccac ctgaagaaac c                                               21


<210>  80
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-16-for-in"

<400>  80
tccttccacc tgaagaaacc                                                 20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-20-for-in"

<400>  81
acgtggtacc aacagtttcc                                                 20


<210>  82
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-27-for-in"

<400>  82
acttcagaca gactgtattg g                                               21


<210>  83
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer V 
       alpha-30-for-in"

<400>  83
ctcttcaccc tgtattcagc                                                 20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer pcDNA-for-1"

<400>  84
cactgcttac tggcttatcg                                                 20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer pcDNA-rev-1"

<400>  85
actagaaggc acagtcgagg                                                 20


<210>  86
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNA-for-2-TOPO(Mimo 1)"

<400>  86
cacccgactc actataggga gacc                                            24


<210>  87
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNA-2nd-for-TOPO(Mimo 2,3)"

<400>  87
cacctccggc gcgccaccat g                                               21


<210>  88
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNA-rev-2(Mimo 1)"

<400>  88
ctgatcagcg ggtttaaact c                                               21


<210>  89
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNA-rev-3(Mimo 2, 3)"

<400>  89
tggtgatggt gatgatgacc                                                 20


<210>  90
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer pcDNA-2nd-for"

<400>  90
tccggcgcgc caccatg                                                    17


<210>  91
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNA-2nd-rev-10"

<400>  91
ctagactcga gcggccgc                                                   18


<210>  92
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       JM22-Valpha-for"

<400>  92
tgtgtcgaca tggtcctgaa attctccgtg                                      30


<210>  93
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       JM22-VJalpha-rev"

<400>  93
ttgggatccc gctcctgcac agaggtagtg gc                                   32


<210>  94
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       JM22-Jalpha-for"

<400>  94
gagggatccc aaggaaatct catctttgga aaa                                  33


<210>  95
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer Calpha 
       PvuII-rev"

<400>  95
agcactgttg ctcttgaagt c                                               21


<210>  96
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       JM22-Vbeta-Kpn-for"

<400>  96
catgtactgg taccgacagg                                                 20


<210>  97
<211>  52
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       JM22-VJbeta-Sca-rev"

<400>  97
ccgaagtact gctcgtagga gctcctcgaa ctactggcac agagatagaa ag             52


<210>  98
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer hCD8a-Not-for"

<400>  98
ataagaatgc ggccgcatgg ccttaccagt gaccgc                               36


<210>  99
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       hCD8a-EcoRI-rev"

<400>  99
ggaattctta gacgtatcac gccgaaag                                        28


<210>  100
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer hCD8b-Mlu-Mut"

<400>  100
tcgacgcgta tgcgaccgcg gctgtgg                                         27


<210>  101
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer hCD8b-Xho-rev"

<400>  101
ccgctcgagt tatttgtaaa attgtttcat gaaac                                35


<210>  102
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer sGFP-Eco-for"

<400>  102
tcgaattcgc caccatggtg agc                                             23


<210>  103
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer sGFP-Xho-rev"

<400>  103
gactcgagag cttagtgatg gtgatg                                          26


<210>  104
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primerHLA-A0101-lead"

<400>  104
ctcgtcgaca tggccgtcat ggcgccc                                         27


<210>  105
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer HLA-A0101-end"

<400>  105
tacggatcct cacactttac aagctgtgag                                      30


<210>  106
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNArc-MCS-for"

<400>  106
gatccggcgc gcccctgcag ggc                                             23


<210>  107
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       pcDNArc-MCS-rev"

<400>  107
ggccgccctg caggggcgcg ccg                                             23


<210>  108
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       N27-flu-Asc-for"

<400>  108
cgcgccacca tgggcattct tgggtttgtg ttcactctgt gagc                      44


<210>  109
<211>  44
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       N27-flu-Not-rev"

<400>  109
ggccgctcac agagtgaaca caaacccaag aatgcccatg gtgg                      44


<210>  110
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       N27-all-lib-Asc-for"


<220>  
<221>  variation
<222>  22
<223>  /replace="t"

<220>  
<221>  variation
<222>  23
<223>  /replace="t"

<220>  
<221>  variation
<222>  24
<223>  /replace="t"

<220>  
<221>  variation
<222>  25
<223>  /replace="t"

<220>  
<221>  variation
<222>  26
<223>  /replace="t"

<220>  
<221>  variation
<222>  27
<223>  /replace="t"

<220>  
<221>  variation
<222>  28
<223>  /replace="t"

<220>  
<221>  variation
<222>  29
<223>  /replace="t"

<220>  
<221>  variation
<222>  30
<223>  /replace="t"

<220>  
<221>  variation
<222>  31
<223>  /replace="t"

<220>  
<221>  variation
<222>  32
<223>  /replace="t"

<220>  
<221>  variation
<222>  33
<223>  /replace="t"

<220>  
<221>  variation
<222>  34
<223>  /replace="t"

<220>  
<221>  variation
<222>  35
<223>  /replace="t"

<220>  
<221>  variation
<222>  36
<223>  /replace="t"

<220>  
<221>  variation
<222>  37
<223>  /replace="t"

<220>  
<221>  variation
<222>  38
<223>  /replace="t"

<220>  
<221>  variation
<222>  39
<223>  /replace="t"

<220>  
<221>  variation
<222>  40
<223>  /replace="t"

<220>  
<221>  variation
<222>  41
<223>  /replace="t"

<220>  
<221>  variation
<222>  42
<223>  /replace="t"

<220>  
<221>  variation
<222>  43
<223>  /replace="t"

<220>  
<221>  variation
<222>  44
<223>  /replace="t"

<220>  
<221>  variation
<222>  45
<223>  /replace="t"

<220>  
<221>  variation
<222>  46
<223>  /replace="t"

<220>  
<221>  variation
<222>  47
<223>  /replace="t"

<220>  
<221>  variation
<222>  48
<223>  /replace="t"

<400>  110
cagggaaggc gcgccaccat gaagaagaag aagaagaaga agaagaagtg agcggccgct     60

aaactat                                                               67


<210>  111
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer 
       N27-A2-269-lib-Asc-for"


<220>  
<221>  variation
<222>  22
<223>  /replace="t"

<220>  
<221>  variation
<222>  23
<223>  /replace="t"

<220>  
<221>  variation
<222>  24
<223>  /replace="t"

<220>  
<221>  variation
<222>  28
<223>  /replace="t"

<220>  
<221>  variation
<222>  29
<223>  /replace="t"

<220>  
<221>  variation
<222>  30
<223>  /replace="t"

<220>  
<221>  variation
<222>  31
<223>  /replace="t"

<220>  
<221>  variation
<222>  32
<223>  /replace="t"

<220>  
<221>  variation
<222>  33
<223>  /replace="t"

<220>  
<221>  variation
<222>  34
<223>  /replace="t"

<220>  
<221>  variation
<222>  35
<223>  /replace="t"

<220>  
<221>  variation
<222>  36
<223>  /replace="t"

<220>  
<221>  variation
<222>  40
<223>  /replace="t"

<220>  
<221>  variation
<222>  41
<223>  /replace="t"

<220>  
<221>  variation
<222>  42
<223>  /replace="t"

<220>  
<221>  variation
<222>  43
<223>  /replace="t"

<220>  
<221>  variation
<222>  44
<223>  /replace="t"

<220>  
<221>  variation
<222>  45
<223>  /replace="t"

<400>  111
cagggaaggc gcgccaccat gaagatcaag aagaaggtga agaagctatg agcggccgct     60

aaactat                                                               67


<210>  112
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: primer N27-Not-rev"

<400>  112
tagtttagcg gccgctca                                                   18


