                         SEQUENCE LISTING

<110>  Biopharm Gesellschaft zur biotechnologischen Entwicklung 
       von Pharmaka mbH
 
<120>  Drug delivery devices and growth factor formulations for 
       accelerated wound healing

<130>  48756P EP

<160>  4     

<170>  PatentIn version 3.3

<210>  1
<211>  2703
<212>  DNA
<213>  homo sapiens


<220>
<221>  CDS
<222>  (640)..(2142)
<223>  GDF-5 precursor

<400>  1
ccatggcctc gaaagggcag cggtgatttt tttcacataa atatatcgca cttaaatgag     60

tttagacagc atgacatcag agagtaatta aattggtttg ggttggaatt ccgtttccaa    120

ttcctgagtt caggtttgta aaagattttt ctgagcacct gcaggcctgt gagtgtgtgt    180

gtgtgtgtgt gtgtgtgtgt gtgtgtgtga agtattttca ctggaaagga ttcaaaacta    240

gggggaaaaa aaaactggag cacacaggca gcattacgcc attcttcctt cttggaaaaa    300

tccctcagcc ttatacaagc ctccttcaag ccctcagtca gttgtgcagg agaaaggggg    360

cggttggctt tctcctttca agaacgagtt attttcagct gctgactgga gacggtgcac    420

gtctggatac gagagcattt ccactatggg actggataca aacacacacc cggcagactt    480

caagagtctc agactgagga gaaagccttt ccttctgctg ctactgctgc tgccgctgct    540

tttgaaagtc cactcctttc atggtttttc ctgccaaacc agaggcacct ttgctgctgc    600

cgctgttctc tttggtgtca ttcagcggct ggccagagg atg aga ctc ccc aaa       654
                                           Met Arg Leu Pro Lys          
                                           1               5            

ctc ctc act ttc ttg ctt tgg tac ctg gct tgg ctg gac ctg gaa ttc      702
Leu Leu Thr Phe Leu Leu Trp Tyr Leu Ala Trp Leu Asp Leu Glu Phe         
                10                  15                  20              

atc tgc act gtg ttg ggt gcc cct gac ttg ggc cag aga ccc cag ggg      750
Ile Cys Thr Val Leu Gly Ala Pro Asp Leu Gly Gln Arg Pro Gln Gly         
            25                  30                  35                  

acc agg cca gga ttg gcc aaa gca gag gcc aag gag agg ccc ccc ctg      798
Thr Arg Pro Gly Leu Ala Lys Ala Glu Ala Lys Glu Arg Pro Pro Leu         
        40                  45                  50                      

gcc cgg aac gtc ttc agg cca ggg ggt cac agc tat ggt ggg ggg gcc      846
Ala Arg Asn Val Phe Arg Pro Gly Gly His Ser Tyr Gly Gly Gly Ala         
    55                  60                  65                          

acc aat gcc aat gcc agg gca aag gga ggc acc ggg cag aca gga ggc      894
Thr Asn Ala Asn Ala Arg Ala Lys Gly Gly Thr Gly Gln Thr Gly Gly         
70                  75                  80                  85          

ctg aca cag ccc aag aag gat gaa ccc aaa aag ctg ccc ccc aga ccg      942
Leu Thr Gln Pro Lys Lys Asp Glu Pro Lys Lys Leu Pro Pro Arg Pro         
                90                  95                  100             

ggc ggc cct gaa ccc aag cca gga cac cct ccc caa aca agg cag gct      990
Gly Gly Pro Glu Pro Lys Pro Gly His Pro Pro Gln Thr Arg Gln Ala         
            105                 110                 115                 

aca gcc cgg act gtg acc cca aaa gga cag ctt ccc gga ggc aag gca     1038
Thr Ala Arg Thr Val Thr Pro Lys Gly Gln Leu Pro Gly Gly Lys Ala         
        120                 125                 130                     

ccc cca aaa gca gga tct gtc ccc agc tcc ttc ctg ctg aag aag gcc     1086
Pro Pro Lys Ala Gly Ser Val Pro Ser Ser Phe Leu Leu Lys Lys Ala         
    135                 140                 145                         

agg gag ccc ggg ccc cca cga gag ccc aag gag ccg ttt cgc cca ccc     1134
Arg Glu Pro Gly Pro Pro Arg Glu Pro Lys Glu Pro Phe Arg Pro Pro         
150                 155                 160                 165         

ccc atc aca ccc cac gag tac atg ctc tcg ctg tac agg acg ctg tcc     1182
Pro Ile Thr Pro His Glu Tyr Met Leu Ser Leu Tyr Arg Thr Leu Ser         
                170                 175                 180             

gat gct gac aga aag gga ggc aac agc agc gtg aag ttg gag gct ggc     1230
Asp Ala Asp Arg Lys Gly Gly Asn Ser Ser Val Lys Leu Glu Ala Gly         
            185                 190                 195                 

ctg gcc aac acc atc acc agc ttt att gac aaa ggg caa gat gac cga     1278
Leu Ala Asn Thr Ile Thr Ser Phe Ile Asp Lys Gly Gln Asp Asp Arg         
        200                 205                 210                     

ggt ccc gtg gtc agg aag cag agg tac gtg ttt gac att agt gcc ctg     1326
Gly Pro Val Val Arg Lys Gln Arg Tyr Val Phe Asp Ile Ser Ala Leu         
    215                 220                 225                         

gag aag gat ggg ctg ctg ggg gcc gag ctg cgg atc ttg cgg aag aag     1374
Glu Lys Asp Gly Leu Leu Gly Ala Glu Leu Arg Ile Leu Arg Lys Lys         
230                 235                 240                 245         

ccc tcg gac acg gcc aag cca gcg gcc ccc gga ggc ggg cgg gct gcc     1422
Pro Ser Asp Thr Ala Lys Pro Ala Ala Pro Gly Gly Gly Arg Ala Ala         
                250                 255                 260             

cag ctg aag ctg tcc agc tgc ccc agc ggc cgg cag ccg gcc tcc ttg     1470
Gln Leu Lys Leu Ser Ser Cys Pro Ser Gly Arg Gln Pro Ala Ser Leu         
            265                 270                 275                 

ctg gat gtg cgc tcc gtg cca ggc ctg gac gga tct ggc tgg gag gtg     1518
Leu Asp Val Arg Ser Val Pro Gly Leu Asp Gly Ser Gly Trp Glu Val         
        280                 285                 290                     

ttc gac atc tgg aag ctc ttc cga aac ttt aag aac tcg gcc cag ctg     1566
Phe Asp Ile Trp Lys Leu Phe Arg Asn Phe Lys Asn Ser Ala Gln Leu         
    295                 300                 305                         

tgc ctg gag ctg gag gcc tgg gaa cgg ggc agg gcc gtg gac ctc cgt     1614
Cys Leu Glu Leu Glu Ala Trp Glu Arg Gly Arg Ala Val Asp Leu Arg         
310                 315                 320                 325         

ggc ctg ggc ttc gac cgc gcc gcc cgg cag gtc cac gag aag gcc ctg     1662
Gly Leu Gly Phe Asp Arg Ala Ala Arg Gln Val His Glu Lys Ala Leu         
                330                 335                 340             

ttc ctg gtg ttt ggc cgc acc aag aaa cgg gac ctg ttc ttt aat gag     1710
Phe Leu Val Phe Gly Arg Thr Lys Lys Arg Asp Leu Phe Phe Asn Glu         
            345                 350                 355                 

att aag gcc cgc tct ggc cag gac gat aag acc gtg tat gag tac ctg     1758
Ile Lys Ala Arg Ser Gly Gln Asp Asp Lys Thr Val Tyr Glu Tyr Leu         
        360                 365                 370                     

ttc agc cag cgg cga aaa cgg cgg gcc cca ctg gcc act cgc cag ggc     1806
Phe Ser Gln Arg Arg Lys Arg Arg Ala Pro Leu Ala Thr Arg Gln Gly         
    375                 380                 385                         

aag cga ccc agc aag aac ctt aag gct cgc tgc agt cgg aag gca ctg     1854
Lys Arg Pro Ser Lys Asn Leu Lys Ala Arg Cys Ser Arg Lys Ala Leu         
390                 395                 400                 405         

cat gtc aac ttc aag gac atg ggc tgg gac gac tgg atc atc gca ccc     1902
His Val Asn Phe Lys Asp Met Gly Trp Asp Asp Trp Ile Ile Ala Pro         
                410                 415                 420             

ctt gag tac gag gct ttc cac tgc gag ggg ctg tgc gag ttc cca ttg     1950
Leu Glu Tyr Glu Ala Phe His Cys Glu Gly Leu Cys Glu Phe Pro Leu         
            425                 430                 435                 

cgc tcc cac ctg gag ccc acg aat cat gca gtc atc cag acc ctg atg     1998
Arg Ser His Leu Glu Pro Thr Asn His Ala Val Ile Gln Thr Leu Met         
        440                 445                 450                     

aac tcc atg gac ccc gag tcc aca cca ccc acc tgc tgt gtg ccc acg     2046
Asn Ser Met Asp Pro Glu Ser Thr Pro Pro Thr Cys Cys Val Pro Thr         
    455                 460                 465                         

cgg ctg agt ccc atc agc atc ctc ttc att gac tct gcc aac aac gtg     2094
Arg Leu Ser Pro Ile Ser Ile Leu Phe Ile Asp Ser Ala Asn Asn Val         
470                 475                 480                 485         

gtg tat aag cag tat gag gac atg gtc gtg gag tcg tgt ggc tgc agg     2142
Val Tyr Lys Gln Tyr Glu Asp Met Val Val Glu Ser Cys Gly Cys Arg         
                490                 495                 500             

tagcagcact ggccctctgt cttcctgggt ggcacatccc aagagcccct tcctgcactc   2202

ctggaatcac agaggggtca ggaagctgtg gcaggagcat ctacacagct tgggtgaaag   2262

gggattccaa taagcttgct cgctctctga gtgtgacttg ggctaaaggc ccccttttat   2322

ccacaagttc ccctggctga ggattgctgc ccgtctgctg atgtgaccag tggcaggcac   2382

aggtccaggg agacagactc tgaatgggac tgagtcccag gaaacagtgc tttccgatga   2442

gactcagccc accatttctc ctcacctggg ccttctcagc ctctggactc tcctaagcac   2502

ctctcaggag agccacaggt gccactgcct cctcaaatca catttgtgcc tggtgacttc   2562

ctgtccctgg gacagttgag aagctgactg ggcaagagtg ggagagaaga ggagagggct   2622

tggatagagt tgaggagtgt gaggctgtta gactgttaga tttaaatgta tattgatgag   2682

ataaaaagca aaactgtgcc t                                             2703


<210>  2
<211>  501
<212>  PRT
<213>  homo sapiens

<400>  2

Met Arg Leu Pro Lys Leu Leu Thr Phe Leu Leu Trp Tyr Leu Ala Trp 
1               5                   10                  15      


Leu Asp Leu Glu Phe Ile Cys Thr Val Leu Gly Ala Pro Asp Leu Gly 
            20                  25                  30          


Gln Arg Pro Gln Gly Thr Arg Pro Gly Leu Ala Lys Ala Glu Ala Lys 
        35                  40                  45              


Glu Arg Pro Pro Leu Ala Arg Asn Val Phe Arg Pro Gly Gly His Ser 
    50                  55                  60                  


Tyr Gly Gly Gly Ala Thr Asn Ala Asn Ala Arg Ala Lys Gly Gly Thr 
65                  70                  75                  80  


Gly Gln Thr Gly Gly Leu Thr Gln Pro Lys Lys Asp Glu Pro Lys Lys 
                85                  90                  95      


Leu Pro Pro Arg Pro Gly Gly Pro Glu Pro Lys Pro Gly His Pro Pro 
            100                 105                 110         


Gln Thr Arg Gln Ala Thr Ala Arg Thr Val Thr Pro Lys Gly Gln Leu 
        115                 120                 125             


Pro Gly Gly Lys Ala Pro Pro Lys Ala Gly Ser Val Pro Ser Ser Phe 
    130                 135                 140                 


Leu Leu Lys Lys Ala Arg Glu Pro Gly Pro Pro Arg Glu Pro Lys Glu 
145                 150                 155                 160 


Pro Phe Arg Pro Pro Pro Ile Thr Pro His Glu Tyr Met Leu Ser Leu 
                165                 170                 175     


Tyr Arg Thr Leu Ser Asp Ala Asp Arg Lys Gly Gly Asn Ser Ser Val 
            180                 185                 190         


Lys Leu Glu Ala Gly Leu Ala Asn Thr Ile Thr Ser Phe Ile Asp Lys 
        195                 200                 205             


Gly Gln Asp Asp Arg Gly Pro Val Val Arg Lys Gln Arg Tyr Val Phe 
    210                 215                 220                 


Asp Ile Ser Ala Leu Glu Lys Asp Gly Leu Leu Gly Ala Glu Leu Arg 
225                 230                 235                 240 


Ile Leu Arg Lys Lys Pro Ser Asp Thr Ala Lys Pro Ala Ala Pro Gly 
                245                 250                 255     


Gly Gly Arg Ala Ala Gln Leu Lys Leu Ser Ser Cys Pro Ser Gly Arg 
            260                 265                 270         


Gln Pro Ala Ser Leu Leu Asp Val Arg Ser Val Pro Gly Leu Asp Gly 
        275                 280                 285             


Ser Gly Trp Glu Val Phe Asp Ile Trp Lys Leu Phe Arg Asn Phe Lys 
    290                 295                 300                 


Asn Ser Ala Gln Leu Cys Leu Glu Leu Glu Ala Trp Glu Arg Gly Arg 
305                 310                 315                 320 


Ala Val Asp Leu Arg Gly Leu Gly Phe Asp Arg Ala Ala Arg Gln Val 
                325                 330                 335     


His Glu Lys Ala Leu Phe Leu Val Phe Gly Arg Thr Lys Lys Arg Asp 
            340                 345                 350         


Leu Phe Phe Asn Glu Ile Lys Ala Arg Ser Gly Gln Asp Asp Lys Thr 
        355                 360                 365             


Val Tyr Glu Tyr Leu Phe Ser Gln Arg Arg Lys Arg Arg Ala Pro Leu 
    370                 375                 380                 


Ala Thr Arg Gln Gly Lys Arg Pro Ser Lys Asn Leu Lys Ala Arg Cys 
385                 390                 395                 400 


Ser Arg Lys Ala Leu His Val Asn Phe Lys Asp Met Gly Trp Asp Asp 
                405                 410                 415     


Trp Ile Ile Ala Pro Leu Glu Tyr Glu Ala Phe His Cys Glu Gly Leu 
            420                 425                 430         


Cys Glu Phe Pro Leu Arg Ser His Leu Glu Pro Thr Asn His Ala Val 
        435                 440                 445             


Ile Gln Thr Leu Met Asn Ser Met Asp Pro Glu Ser Thr Pro Pro Thr 
    450                 455                 460                 


Cys Cys Val Pro Thr Arg Leu Ser Pro Ile Ser Ile Leu Phe Ile Asp 
465                 470                 475                 480 


Ser Ala Asn Asn Val Val Tyr Lys Gln Tyr Glu Asp Met Val Val Glu 
                485                 490                 495     


Ser Cys Gly Cys Arg 
            500     


<210>  3
<211>  360
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(360)
<223>  human mature monomeric GDF-5

<400>  3
gca cca cta gca act cgt cag ggc aag cga ccc agc aag aac ctt aag       48
Ala Pro Leu Ala Thr Arg Gln Gly Lys Arg Pro Ser Lys Asn Leu Lys         
1               5                   10                  15              

gct cgc tgc agt cgg aag gca ctg cat gtc aac ttc aag gac atg ggc       96
Ala Arg Cys Ser Arg Lys Ala Leu His Val Asn Phe Lys Asp Met Gly         
            20                  25                  30                  

tgg gac gac tgg atc atc gca ccc ctt gag tac gag gct ttc cac tgc      144
Trp Asp Asp Trp Ile Ile Ala Pro Leu Glu Tyr Glu Ala Phe His Cys         
        35                  40                  45                      

gag ggg ctg tgc gag ttc cca ttg cgc tcc cac ctg gag ccc acg aat      192
Glu Gly Leu Cys Glu Phe Pro Leu Arg Ser His Leu Glu Pro Thr Asn         
    50                  55                  60                          

cat gca gtc atc cag acc ctg atg aac tcc atg gac ccc gag tcc aca      240
His Ala Val Ile Gln Thr Leu Met Asn Ser Met Asp Pro Glu Ser Thr         
65                  70                  75                  80          

cca ccc acc gcc tgt gtg ccc acg cga ctg agt ccc atc agc atc ctc      288
Pro Pro Thr Ala Cys Val Pro Thr Arg Leu Ser Pro Ile Ser Ile Leu         
                85                  90                  95              

ttc att gac tct gcc aac aac gtg gtg tat aag cag tat gag gac atg      336
Phe Ile Asp Ser Ala Asn Asn Val Val Tyr Lys Gln Tyr Glu Asp Met         
            100                 105                 110                 

gtc gtg gag tcg tgt ggc tgt agg                                      360
Val Val Glu Ser Cys Gly Cys Arg                                         
        115                 120                                         


<210>  4
<211>  120
<212>  PRT
<213>  Homo sapiens

<400>  4

Ala Pro Leu Ala Thr Arg Gln Gly Lys Arg Pro Ser Lys Asn Leu Lys 
1               5                   10                  15      


Ala Arg Cys Ser Arg Lys Ala Leu His Val Asn Phe Lys Asp Met Gly 
            20                  25                  30          


Trp Asp Asp Trp Ile Ile Ala Pro Leu Glu Tyr Glu Ala Phe His Cys 
        35                  40                  45              


Glu Gly Leu Cys Glu Phe Pro Leu Arg Ser His Leu Glu Pro Thr Asn 
    50                  55                  60                  


His Ala Val Ile Gln Thr Leu Met Asn Ser Met Asp Pro Glu Ser Thr 
65                  70                  75                  80  


Pro Pro Thr Ala Cys Val Pro Thr Arg Leu Ser Pro Ile Ser Ile Leu 
                85                  90                  95      


Phe Ile Asp Ser Ala Asn Asn Val Val Tyr Lys Gln Tyr Glu Asp Met 
            100                 105                 110         


Val Val Glu Ser Cys Gly Cys Arg 
        115                 120 


