                         SEQUENCE LISTING

<110>  The Secretary of State for Environment, Food and Rural 
       Affairs acting through the Animal Health and Veterinary 
       Laboratories Agency
       Jones, Gareth J
       Vordermeier, Hanns M
 
<120>  Diagnostic Reagents

<130>  RT/P1821GB00

<150>  GB1012072.3
<151>  2010-07-19

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  1

Met Trp Ala Ser Ala Gln Asn Ile Ser Gly Ala Gly Trp Ser Gly Met 
1               5                   10                  15      


Ala Glu Ala Thr 
            20  


<210>  2
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  2

Met Thr Gln Met Asn Gln Ala Phe Arg Asn Ile Val Asn Met Leu His 
1               5                   10                  15      


Gly Val Arg Asp 
            20  


<210>  3
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  3

Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Val Arg 
1               5                   10                  15      


Asp Ala Asn Asn 
            20  


<210>  4
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  4

Met Ala Gln Met Asn Gln Ala Phe Arg Asn Ile Val Asn Met Leu His 
1               5                   10                  15      


Gly Val Arg Asp 
            20  


<210>  5
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  5

Glu Ala Glu His Gln Ala Ile Ile Arg Asp Val Leu Thr Ala Ser Asp 
1               5                   10                  15      


Phe Trp Gly Gly 
            20  


<210>  6
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  6

Leu Gly Arg Asn Phe Gln Val Ile Tyr Glu Gln Ala Asn Ala His Gly 
1               5                   10                  15      


Gln Lys Val Gln 
            20  


<210>  7
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  7

Arg Asp Val Leu Ala Ala Gly Asp Phe Trp Gly Gly Ala Gly Ser Val 
1               5                   10                  15      


Ala Cys Gln Glu 
            20  


<210>  8
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  8

Gln Ala Asn Leu Gly Glu Ala Ala Gly Thr Tyr Val Ala Ala Asp Ala 
1               5                   10                  15      


Ala Ala Ala Ser 
            20  


<210>  9
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  9

Asp Val Asp Ala His Gly Ala Met Ile Arg Ala Gln Ala Gly Ser Leu 
1               5                   10                  15      


Glu Ala Glu His 
            20  


<210>  10
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  10

Ser Ala Glu Leu Pro Asp Trp Leu Ala Ala Asn Arg Gly Leu Ala Pro 
1               5                   10                  15      


Gly Gly His Ala 
            20  


<210>  11
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  11

Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15      


<210>  12
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  12

Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser 
1               5                   10                  15      


<210>  13
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  13

Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
1               5                   10                  15      


<210>  14
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  14

Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala 
1               5                   10                  15      


<210>  15
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  15

Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
1               5                   10                  15      


<210>  16
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  16

Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln 
1               5                   10                  15      


<210>  17
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  17

Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
1               5                   10                  15      


<210>  18
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  18

Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu 
1               5                   10                  15      


<210>  19
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  19

Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
1               5                   10                  15      


<210>  20
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  20

Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly 
1               5                   10                  15      


<210>  21
<211>  15
<212>  PRT
<213>  Mycobacterium bovis

<400>  21

Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala 
1               5                   10                  15  


<210>  22
<211>  18
<212>  PRT
<213>  Mycobacterium bovis

<400>  22

Met Ala Glu Met Lys Thr Asp Ala Ala Thr Leu Ala Gln Glu Ala Gly 
1               5                   10                  15      


Asn Phe 
        


<210>  23
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  23

Gln Glu Ala Gly Asn Phe Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln 
1               5                   10                  15      


<210>  24
<211>  18
<212>  PRT
<213>  Mycobacterium bovis

<400>  24

Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln Ile Asp Gln Val Glu Ser 
1               5                   10                  15      


Thr Ala 
        


<210>  25
<211>  17
<212>  PRT
<213>  Mycobacterium bovis

<400>  25

Ile Asp Gln Val Glu Ser Thr Ala Gly Ser Leu Gln Gly Gln Trp Arg 
1               5                   10                  15      


Gly 
    


<210>  26
<211>  18
<212>  PRT
<213>  Mycobacterium bovis

<400>  26

Gly Ser Leu Gln Gly Gln Trp Arg Gly Ala Ala Gly Thr Ala Ala Gln 
1               5                   10                  15      


Ala Ala 
        


<210>  27
<211>  18
<212>  PRT
<213>  Mycobacterium bovis

<400>  27

Ala Gly Thr Ala Ala Gln Ala Ala Val Val Arg Phe Gln Glu Ala Ala 
1               5                   10                  15      


Asn Lys 
        


<210>  28
<211>  18
<212>  PRT
<213>  Mycobacterium bovis

<400>  28

Val Val Arg Phe Gln Glu Ala Ala Asn Lys Gln Lys Gln Glu Leu Asp 
1               5                   10                  15      


Glu Ile 
        


<210>  29
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  29

Gln Lys Gln Glu Leu Asp Glu Ile Ser Thr Asn Ile Arg Gln Ala Gly 
1               5                   10                  15      


Val Gln Tyr Ser 
            20  


<210>  30
<211>  18
<212>  PRT
<213>  Mycobacterium bovis

<400>  30

Asn Ile Arg Gln Ala Gly Val Gln Tyr Ser Arg Ala Asp Glu Glu Gln 
1               5                   10                  15      


Gln Gln 
        


<210>  31
<211>  16
<212>  PRT
<213>  Mycobacterium bovis

<400>  31

Arg Ala Asp Glu Glu Gln Gln Gln Ala Leu Ser Ser Gln Met Gly Phe 
1               5                   10                  15      


<210>  32
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  32

Met Thr Glu Asn Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala 
1               5                   10                  15      


Ser His His Asp 
            20  


<210>  33
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  33

Pro Glu Arg Leu Gly Val Leu Ala Ser His His Asp Asn Ala Ala Val 
1               5                   10                  15      


Asp Ala Ser Ser 
            20  


<210>  34
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  34

Ser His His Asp Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala 
1               5                   10                  15      


Ala Ala Gly Leu 
            20  


<210>  35
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  35

Asp Ala Ser Ser Gly Val Glu Ala Ala Ala Gly Leu Gly Glu Ser Val 
1               5                   10                  15      


Ala Ile Thr His 
            20  


<210>  36
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  36

Ala Ala Gly Leu Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys 
1               5                   10                  15      


Ser Gln Phe Asn 
            20  


<210>  37
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  37

Ala Ile Thr His Gly Pro Tyr Cys Ser Gln Phe Asn Asp Thr Leu Asn 
1               5                   10                  15      


Val Tyr Leu Thr 
            20  


<210>  38
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  38

Ser Gln Phe Asn Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala 
1               5                   10                  15      


Leu Gly Ser Ser 
            20  


<210>  39
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  39

Val Tyr Leu Thr Ala His Asn Ala Leu Gly Ser Ser Leu His Thr Ala 
1               5                   10                  15      


Gly Val Asp Leu 
            20  


<210>  40
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  40

Leu Gly Ser Ser Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu 
1               5                   10                  15      


Arg Ile Ala Ala 
            20  


<210>  41
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  41

Gly Val Asp Leu Ala Lys Ser Leu Arg Ile Ala Ala Lys Ile Tyr Ser 
1               5                   10                  15      


Glu Ala Asp Glu 
            20  


<210>  42
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  42

Arg Ile Ala Ala Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys 
1               5                   10                  15      


Ala Ile Asp Gly 
            20  


<210>  43
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  43

Ala Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys Ala Ile Asp 
1               5                   10                  15      


Gly Leu Phe Tyr 
            20  


<210>  44
<211>  95
<212>  PRT
<213>  Mycobacterium bovis

<400>  44

Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser 
1               5                   10                  15      


Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly 
            20                  25                  30          


Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser 
        35                  40                  45              


Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu 
    50                  55                  60                  


Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly 
65                  70                  75                  80  


Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala 
                85                  90                  95  


<210>  45
<211>  100
<212>  PRT
<213>  Mycobacterium bovis

<400>  45

Met Ala Glu Met Lys Thr Asp Ala Ala Thr Leu Ala Gln Glu Ala Gly 
1               5                   10                  15      


Asn Phe Glu Arg Ile Ser Gly Asp Leu Lys Thr Gln Ile Asp Gln Val 
            20                  25                  30          


Glu Ser Thr Ala Gly Ser Leu Gln Gly Gln Trp Arg Gly Ala Ala Gly 
        35                  40                  45              


Thr Ala Ala Gln Ala Ala Val Val Arg Phe Gln Glu Ala Ala Asn Lys 
    50                  55                  60                  


Gln Lys Gln Glu Leu Asp Glu Ile Ser Thr Asn Ile Arg Gln Ala Gly 
65                  70                  75                  80  


Val Gln Tyr Ser Arg Ala Asp Glu Glu Gln Gln Gln Ala Leu Ser Ser 
                85                  90                  95      


Gln Met Gly Phe 
            100 


<210>  46
<211>  103
<212>  PRT
<213>  Mycobacterium bovis

<400>  46

Met Thr Glu Asn Leu Thr Val Gln Pro Glu Arg Leu Gly Val Leu Ala 
1               5                   10                  15      


Ser His His Asp Asn Ala Ala Val Asp Ala Ser Ser Gly Val Glu Ala 
            20                  25                  30          


Ala Ala Gly Leu Gly Glu Ser Val Ala Ile Thr His Gly Pro Tyr Cys 
        35                  40                  45              


Ser Gln Phe Asn Asp Thr Leu Asn Val Tyr Leu Thr Ala His Asn Ala 
    50                  55                  60                  


Leu Gly Ser Ser Leu His Thr Ala Gly Val Asp Leu Ala Lys Ser Leu 
65                  70                  75                  80  


Arg Ile Ala Ala Lys Ile Tyr Ser Glu Ala Asp Glu Ala Trp Arg Lys 
                85                  90                  95      


Ala Ile Asp Gly Leu Phe Thr 
            100             


<210>  47
<211>  220
<212>  PRT
<213>  Mycobacterium bovis

<400>  47

Met Ile Asn Val Gln Ala Lys Pro Ala Ala Ala Ala Ser Leu Ala Ala 
1               5                   10                  15      


Ile Ala Ile Ala Phe Leu Ala Gly Cys Ser Ser Thr Lys Pro Val Ser 
            20                  25                  30          


Gln Asp Thr Ser Pro Lys Pro Ala Thr Ser Pro Ala Ala Pro Val Thr 
        35                  40                  45              


Thr Ala Ala Met Ala Asp Pro Ala Ala Asp Leu Ile Gly Arg Gly Cys 
    50                  55                  60                  


Ala Gln Tyr Ala Ala Gln Asn Pro Thr Gly Pro Gly Ser Val Ala Gly 
65                  70                  75                  80  


Met Ala Gln Asp Pro Val Ala Thr Ala Ala Ser Asn Asn Pro Met Leu 
                85                  90                  95      


Ser Thr Leu Thr Ser Ala Leu Ser Gly Lys Leu Asn Pro Asp Val Asn 
            100                 105                 110         


Leu Val Asp Thr Leu Asn Gly Gly Glu Tyr Thr Val Phe Ala Pro Thr 
        115                 120                 125             


Asn Ala Ala Phe Asp Lys Leu Pro Ala Ala Thr Ile Asp Gln Leu Lys 
    130                 135                 140                 


Thr Asp Ala Lys Leu Leu Ser Ser Ile Leu Thr Tyr His Val Ile Ala 
145                 150                 155                 160 


Gly Gln Ala Ser Pro Ser Arg Ile Asp Gly Thr His Gln Thr Leu Gln 
                165                 170                 175     


Gly Ala Asp Leu Thr Val Ile Gly Ala Arg Asp Asp Leu Met Val Asn 
            180                 185                 190         


Asn Ala Gly Leu Val Cys Gly Gly Val His Thr Ala Asn Ala Thr Val 
        195                 200                 205             


Tyr Met Ile Asp Thr Val Leu Met Pro Pro Ala Gln 
    210                 215                 220 


<210>  48
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  48

Gly Leu Val Cys Gly Gly Val His Thr Ala Asn Ala Thr Val Tyr Met 
1               5                   10                  15      


Ile Asp Thr Val 
            20  


<210>  49
<211>  193
<212>  PRT
<213>  Mycobacterium bovis

<400>  49

Met Lys Val Lys Asn Thr Ile Ala Ala Thr Ser Phe Ala Ala Ala Gly 
1               5                   10                  15      


Leu Ala Ala Leu Ala Val Ala Val Ser Pro Pro Ala Ala Ala Gly Asp 
            20                  25                  30          


Leu Val Gly Pro Gly Cys Ala Glu Tyr Ala Ala Ala Asn Pro Thr Gly 
        35                  40                  45              


Pro Ala Ser Val Gln Gly Met Ser Gln Asp Pro Val Ala Val Ala Ala 
    50                  55                  60                  


Ser Asn Asn Pro Glu Leu Thr Thr Leu Thr Ala Ala Leu Ser Gly Gln 
65                  70                  75                  80  


Leu Asn Pro Gln Val Asn Leu Val Asp Thr Leu Asn Ser Gly Gln Tyr 
                85                  90                  95      


Thr Val Phe Ala Pro Thr Asn Ala Ala Phe Ser Lys Leu Pro Ala Ser 
            100                 105                 110         


Thr Ile Asp Glu Leu Lys Thr Asn Ser Ser Leu Leu Thr Ser Ile Leu 
        115                 120                 125             


Thr Tyr His Val Val Ala Gly Gln Thr Ser Pro Ala Asn Val Val Gly 
    130                 135                 140                 


Thr Arg Gln Thr Leu Gln Gly Ala Ser Val Thr Val Thr Gly Gln Gly 
145                 150                 155                 160 


Asn Ser Leu Lys Val Gly Asn Ala Asp Val Val Cys Gly Gly Val Ser 
                165                 170                 175     


Thr Ala Asn Ala Thr Val Tyr Met Ile Asp Ser Val Leu Met Pro Pro 
            180                 185                 190         


Ala 
    


<210>  50
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  50

Met Thr Ile Asn Tyr Gln Phe Gly Asp Val Asp Ala His Gly Ala Met 
1               5                   10                  15      


Ile Arg Ala Gln 
            20  


<210>  51
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  51

Asp Val Asp Ala His Gly Ala Met Ile Arg Ala Gln Ala Gly Leu Leu 
1               5                   10                  15      


Glu Ala Glu His 
            20  


<210>  52
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  52

Ile Arg Ala Gln Ala Gly Leu Leu Glu Ala Glu His Gln Ala Ile Val 
1               5                   10                  15      


Arg Asp Val Leu 
            20  


<210>  53
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  53

Glu Ala Glu His Gln Ala Ile Val Arg Asp Val Leu Ala Ala Gly Asp 
1               5                   10                  15      


Phe Trp Gly Gly 
            20  


<210>  54
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  54

Phe Trp Gly Gly Ala Gly Ser Val Ala Cys Gln Glu Phe Ile Thr Gln 
1               5                   10                  15      


Leu Gly Arg Asn 
            20  


<210>  55
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  55

Ala Cys Gln Glu Phe Ile Thr Gln Leu Gly Arg Asn Phe Gln Val Ile 
1               5                   10                  15      


Tyr Glu Gln Ala 
            20  


<210>  56
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  56

Leu Gly Arg Asn Phe Gln Val Ile Tyr Glu Gln Ala Asn Ala His Gly 
1               5                   10                  15      


Gln Lys Val Gln 
            20  


<210>  57
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  57

Tyr Glu Gln Ala Asn Ala His Gly Gln Lys Val Gln Ala Ala Gly Asn 
1               5                   10                  15      


Asn Met Ala Gln 
            20  


<210>  58
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  58

Gln Lys Val Gln Ala Ala Gly Asn Asn Met Ala Gln Thr Asp Ser Ala 
1               5                   10                  15      


Val Gly Ser Ser 
            20  


<210>  59
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  59

Val Gln Ala Ala Gly Asn Asn Met Ala Gln Thr Asp Ser Ala Val Gly 
1               5                   10                  15      


Ser Ser Trp Ala 
            20  


<210>  60
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  60

Met Ser Leu Leu Asp Ala His Ile Pro Gln Leu Ile Ala Ser His Thr 
1               5                   10                  15      


Ala Phe Ala Ala 
            20  


<210>  61
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  61

Pro Gln Leu Ile Ala Ser His Thr Ala Phe Ala Ala Lys Ala Gly Leu 
1               5                   10                  15      


Met Arg His Thr 
            20  


<210>  62
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  62

Ala Phe Ala Ala Lys Ala Gly Leu Met Arg His Thr Ile Gly Gln Ala 
1               5                   10                  15      


Glu Gln Gln Ala 
            20  


<210>  63
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  63

Met Arg His Thr Ile Gly Gln Ala Glu Gln Gln Ala Met Ser Ala Gln 
1               5                   10                  15      


Ala Phe His Gln 
            20  


<210>  64
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  64

Glu Gln Gln Ala Met Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ala 
1               5                   10                  15      


Ala Ala Phe Gln 
            20  


<210>  65
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  65

Ala Phe His Gln Gly Glu Ser Ala Ala Ala Phe Gln Gly Ala His Ala 
1               5                   10                  15      


Arg Phe Val Ala 
            20  


<210>  66
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  66

Ala Ala Phe Gln Gly Ala His Ala Arg Phe Val Ala Ala Ala Ala Lys 
1               5                   10                  15      


Val Asn Thr Leu 
            20  


<210>  67
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  67

Arg Phe Val Ala Ala Ala Ala Lys Val Asn Thr Leu Leu Asp Ile Ala 
1               5                   10                  15      


Gln Ala Asn Leu 
            20  


<210>  68
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  68

Val Asn Thr Leu Leu Asp Ile Ala Gln Ala Asn Leu Gly Glu Ala Ala 
1               5                   10                  15      


Gly Thr Tyr Val 
            20  


<210>  69
<211>  20
<212>  PRT
<213>  Mycobacterium bovis

<400>  69

Glu Ala Ala Gly Thr Tyr Val Ala Ala Asp Ala Ala Ala Ala Ser Ser 
1               5                   10                  15      


Tyr Thr Gly Phe 
            20  


