                         SEQUENCE LISTING

<110>  HITACHI CHEMICAL CO., LTD
       HITACHI CHEMICAL RESEARCH CENTER, INC
       Yamamoto, Cindy
 
<120>  DETECTION OF NUCLEIC ACIDS BY AGGLUTINATION

<130>  HITACHI.100WO

<150>  61/364,758
<151>  2010-07-15

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Forward Primer

<400>  1
cgtccatcta tttgccaggt                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Reverse Primer

<400>  2
atttcggata aagcgtggtg                                                   20


<210>  3
<211>  28
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Forward 2'O-methylated RNA primer

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Adenine at position 7 is a 2' O-methyl RNA adenine

<400>  3
aaaaaaatca ctctggagga tacgttgc                                          28


<210>  4
<211>  27
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Reverse 2'O-methylated RNA primer

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Adenine at position 7 is a 2' O-methyl RNA adenine

<400>  4
aaaaaaatta ccgttctcca ccattcc                                           27


<210>  5
<211>  20
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Capture probe

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Position 20 is conjugated to biotin moeity

<400>  5
tttttttttt tttttttttt                                                   20


<210>  6
<211>  28
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Forward "No methyl" primer

<400>  6
aaaaaaatca ctctggagga tacgttgc                                          28


<210>  7
<211>  27
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Reverse "No Methyl" primer

<400>  7
aaaaaaatta ccgttctcca ccattcc                                           27


<210>  8
<211>  21
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Forward "no handle" primer

<400>  8
tcactctgga ggatacgttg c                                                 21


<210>  9
<211>  20
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Reverse "no handle" primer

<400>  9
ttaccgttct ccaccattcc                                                   20


<210>  10
<211>  28
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Forward "Single Stranded Ends" primer

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Adenine at position 7 is a 2' O-methyl RNA adenine

<400>  10
aaaaaaatca ctctggagga tacgttgc                                          28


<210>  11
<211>  28
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Reverse "Single Stranded Ends" primer

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Adenine at position 7 is a 2' O-methyl RNA adenine

<400>  11
aaaaaamatt accgttctcc accattcc                                          28


<210>  12
<211>  20
<212>  DNA
<213>  Listeria monocytogenes


<220>
<221>  misc_feature
<223>  Capture probe

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Position 20 is coupled to a biotin moeity

<400>  12
tttttttttt tttttttttt                                                   20


<210>  13
<211>  73
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Let-7d padlock probe

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The 5' end of the probe is phosphorylated

<400>  13
ctactacctc ttttatttcc tcaatgctgc tgctgtacta ctagtgattt acttggatgt       60

ctaactatgc aaa                                                          73


<210>  14
<211>  70
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Let-7d mutant padlock probe

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  The 5' end of the probe is phosphorylated

<400>  14
ctagtacctc ttttatttcc tcaatgctgc tgtactacta gtgatttact tggatgtcta       60

actatggaac                                                              70


<210>  15
<211>  22
<212>  RNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Let-7d target ribonucleic acid

<400>  15
agagguagua gguugcauag uu                                                22


<210>  16
<211>  16
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Let-7d capture probe

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Position 16 is coupled to a biotin moeity

<400>  16
ctactacctc taaaaa                                                       16


