SEQUENCE LISTING

<110>  Neo VirnaTech S.L.
 
<120>  METHODS AND REAGENTS FOR OBTAINING TRANSCRIPTIONALLY ACTIVE
       VIRUS-LIKE PARTICLES AND RECOMBINANT VIRIONS

<130>  P3924PC00

<150>  EP10382192.2
<151>  2010-07-14

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  96
<212>  DNA
<213>  IBDV

<400>  1
ggatacgatc ggtctgaccc cgggggagtc acccggggac aggtcttcaa ggccttgttc       60

caggatggaa ctcctccttc tacaacgcta tcattg                                 96


<210>  2
<211>  92
<212>  DNA
<213>  IBDV

<400>  2
ggctcctggg agtctcccga caccacccgc gcaggtgtgg acaccaattc ggccttacaa       60

catcccaaat tggatccgtt cgcgggtccc ct                                     92


<210>  3
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence of recognition by the promoter of the T7 phage
       RNA polymerase 

<400>  3
taatacgact cactata                                                      17


<210>  4
<211>  67
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Transcription terminator of the T7 phage RNA polymerase

<400>  4
aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat       60

ccggatc                                                                 67


<210>  5
<211>  159
<212>  DNA
<213>  Hepatitis delta virus

<400>  5
gggtcggcat ggcatctcca cctcctcgcg gtccgacctg ggcatccgaa ggaggacgtc       60

gtccactcgg atggctaagg gagagctcgg atccggctgc taacaaagcc cgaaaggaag      120

ctgagttggc tgctgccacc gctgagcaat aactagcat                             159


<210>  6
<211>  436
<212>  DNA
<213>  IBDV

<400>  6
atggtcagta gagatcagac aaacgatcgc agcgatgaca aacctgcaag atcaaaccca       60

acagattgtt ccgttcatac ggagccttct gatgccaaca accggaccgg cgtccattgt      120

gcatgcagag aagagccggt tggcatcaga agaacaaatc ctaagggcag ctacgtcgat      180

ctacggggct ccaggacagg cagagccacc ccaagctttc atagacgaag ttgccaaagt      240

ctatgaaatc aaccatggac gtggcccaaa ccaagaacag atgaaagatc tgctcttgac      300

tgcgatggag atgaagcatc gcaatcccag gcgggctcta ccaaagccca agccaaaacc      360

caatgctcca acacagagac ctcctggtcg gctgggccgc tggatcagga ccgtctctga      420

tgaggacctt gagtga                                                      436


<210>  7
<211>  365
<212>  DNA
<213>  IBDV

<400>  7
atgagtgacg ttttcaatag tccacaggcg cgaagcacga tctcagcagc gttcggcata       60

aagcctactg ctggacaaga cgtggaagaa ctcttgatcc ctaaagtttg ggtgccacct      120

gaggatccgc ttgccagccc tagtcgactt ccgtgtacac ccccaagtac ccagaagtca      180

agaacccaca gaccgcctcc aaccccgttg ttgggctcca cctgcccgcc aagagagcca      240

ccggtgtcca ggccgctctt ctcggagcag gaacgagcag accaatgggg atggaggccc      300

caacacggtc caagaacgcc gtgaaaatgg ccaaacggcg gcaacgccaa aaggagagcc      360

gctaa                                                                  365


<210>  8
<211>  899
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cas1 chimeric gene sequence

<400>  8
gcggccgcat cgatatatga attctaatac gactcactat aggatacgat cggtctgacc       60

ccgggggagt cacccgggga caggtcttca aggccttgtt ccaggatgga actcctcctt      120

ctacaacgct atcattgatg gtcagtagag atcagacaaa cgatcgcagc gatgacaaac      180

ctgcaagatc aaacccaaca gattgttccg ttcatacgga gccttctgat gccaacaacc      240

ggaccggcgt ccattgtgca tgcagagaag agccggttgg catcagaaga acaaatccta      300

agggcagcta cgtcgatcta cggggctcca ggacaggcag agccacccca agctttcata      360

gacgaagttg ccaaagtcta tgaaatcaac catggacgtg gcccaaacca agaacagatg      420

aaagatctgc tcttgactgc gatggagatg aagcatcgca atcccaggcg ggctctacca      480

aagcccaagc caaaacccaa tgctccaaca cagagacctc ctggtcggct gggccgctgg      540

atcaggaccg tctctgatga ggaccttgag tgaggctcct gggagtctcc cgacaccacc      600

cgcgcaggtg tggacaccaa ttcggcctta caacatccca aattggatcc gttcgcgggt      660

cccctgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg      720

acgtcgtcca ctcggatggc taagggagag ctcggatccg gctgctaaca aagcccgaaa      780

ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc      840

taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat cgcggccgc       899


<210>  9
<211>  3622
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pCas1 plasmid sequence

<400>  9
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tccaatgcgc gcggccgcat cgatatatga attctaatac gactcactat      480

aggatacgat cggtctgacc ccgggggagt cacccgggga caggtcttca aggccttgtt      540

ccaggatgga actcctcctt ctacaacgct atcattgatg gtcagtagag atcagacaaa      600

cgatcgcagc gatgacaaac ctgcaagatc aaacccaaca gattgttccg ttcatacgga      660

gccttctgat gccaacaacc ggaccggcgt ccattgtgca tgcagagaag agccggttgg      720

catcagaaga acaaatccta agggcagcta cgtcgatcta cggggctcca ggacaggcag      780

agccacccca agctttcata gacgaagttg ccaaagtcta tgaaatcaac catggacgtg      840

gcccaaacca agaacagatg aaagatctgc tcttgactgc gatggagatg aagcatcgca      900

atcccaggcg ggctctacca aagcccaagc caaaacccaa tgctccaaca cagagacctc      960

ctggtcggct gggccgctgg atcaggaccg tctctgatga ggaccttgag tgaggctcct     1020

gggagtctcc cgacaccacc cgcgcaggtg tggacaccaa ttcggcctta caacatccca     1080

aattggatcc gttcgcgggt cccctgggtc ggcatggcat ctccacctcc tcgcggtccg     1140

acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag ctcggatccg     1200

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta     1260

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     1320

atatccggat cgcggccgcg cgcatcggat cccgggcccg tcgactgcag aggcctgcat     1380

gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca     1440

attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg     1500

agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg     1560

tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc     1620

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     1680

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     1740

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     1800

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     1860

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     1920

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     1980

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     2040

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     2100

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     2160

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     2220

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     2280

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     2340

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     2400

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     2460

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     2520

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     2580

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     2640

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     2700

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     2760

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     2820

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     2880

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     2940

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     3000

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     3060

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     3120

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     3180

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     3240

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     3300

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     3360

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     3420

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     3480

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     3540

aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg     3600

cgtatcacga ggccctttcg tc                                              3622


<210>  10
<211>  3622
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pHA-Cas1 plasmid sequence

<400>  10
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tccaatgcgc gcggccgcat cgatatatga attctaatac gactcactat      480

aggatacgat cggtctgacc ccgggggagt cacccgggga caggtcttca aggccttgtt      540

ccaggatgga actcctcctt ctacaacgct atcattgatg gtcagtagag atcagacaaa      600

cgatcgcagc gatgacaaac ctgcaagatc aaacccaaca gattgttccg ttcatacgga      660

gccttctgat gccaacaacc ggaccggcgt ccattgtgca tgcagagaag agccggttgg      720

catcagaaga acaaatccta agggcagcta cgtcgatcta cggggctcca ggacaggcag      780

agccacccca agctttcata gacgaagttg ccaaagtcta tgaaatcaac catggacgtg      840

gcccaaacca agaacagatg aaagatctgc tcttgactgc gatggagatg aagcatcgca      900

atcccaggcg ggctctacca aagcccaagc caaaacccaa tgctccaaca cagagacctc      960

ctggtcggct gggccgctgg atcaggaccg tctctgatga ggaccttgag tgaggctcct     1020

gggagtctcc cgacaccacc cgcgcaggtg tggacaccaa ttcggcctta caacatccca     1080

aattggatcc gttcgcgggt cccctgggtc ggcatggcat ctccacctcc tcgcggtccg     1140

acctgggcat ccgaaggagg acgtcgtcca ctcggatggc taagggagag ctcggatccg     1200

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta     1260

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     1320

atatccggat cgcggccgcg cgcatcggat cccgggcccg tcgactgcag aggcctgcat     1380

gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca     1440

attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg     1500

agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg     1560

tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc     1620

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     1680

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     1740

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     1800

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     1860

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     1920

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     1980

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     2040

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     2100

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     2160

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     2220

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     2280

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     2340

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     2400

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     2460

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     2520

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     2580

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     2640

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     2700

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     2760

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     2820

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     2880

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     2940

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     3000

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     3060

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     3120

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     3180

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     3240

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     3300

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     3360

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     3420

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     3480

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     3540

aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg     3600

cgtatcacga ggccctttcg tc                                              3622


<210>  11
<211>  2653
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pFastBac-Poly vector fragment

<400>  11
gcgtccattc cggacgacac cctggagaag cacactctca ggtcagagac ctcgacctac       60

aatttgactg tgggggacac agggtcaggg ctaattgtct ttttccctgg attccctggc      120

tcaattgtgg gtgctcacta cacactgcag ggcaatggga actacaagtt cgatcagatg      180

ctcctgactg cccagaacct accggccagt tacaactact gcaggctagt gagtcggagt      240

ctcacagtga ggtcaagcac acttcctggt ggcgtttatg cactaaacgg caccataaac      300

gccgtgacct tccaaggaag cctgagtgaa ctgacagatg ttagctacaa tgggttgatg      360

tctgcaacag ccaacatcaa cgacaaaatt gggaacgtcc tagtagggga aggggtcacc      420

gtcctcagct tacccacatc atatgatctt gggtatgtga ggcttggtga ccccattccc      480

gcaatagggc ttgacccaaa aatggtagcc acatgtgaca gcagtgacag gcccagagtc      540

tacaccataa ctgcagccga tgattaccaa ttctcatcac agtaccaacc aggtggggta      600

acaatcacac tgttctcagc caacattgat gccatcacaa gcctcagcgt tgggggagag      660

ctcgtgtttc gaacaagcgt ccacggcctt gtactgggcg ccaccatcta cctcataggc      720

tttgatggga caacggtaat caccagggct gtggccgcaa acaatgggct gacgaccggc      780

accgacaacc ttatgccatt caatcttgtg attccaacaa acgagataac ccagccaatc      840

acatccatca aactggagat agtgacctcc aaaagtggtg gtcaggcagg ggatcagatg      900

tcatggtcgg caagagggag cctagcagtg acgatccatg gtggcaacta tccaggggcc      960

ctccgtcccg tcacgctagt ggcctacgaa agagtggcaa caggatccgt cgttacggtc     1020

gctggggtga gcaacttcga gctgatccca aatcctgaac tagcaaagaa cctggttaca     1080

gaatacggcc gatttgaccc aggagccatg aactacacaa aattgatact gagtgagagg     1140

gaccgtcttg gcatcaagac cgtctggcca acaagggagt acactgactt tcgtgaatac     1200

ttcatggagg tggccgacct caactctccc ctgaagattg caggagcatt cggcttcaaa     1260

gacataatcc gggccataag gaggatagct gtgccggtgg tctccacatt gttcccacct     1320

gccgctcccc tagcccatgc aattggggaa ggtgtagact acctgctggg cgatgaggcc     1380

caggccgctt caggaactgc tcgagccgcg tcaggaaaag caagagctgc ctcaggccgc     1440

ataaggcagc tgactctcgc cgccgacaag gggtacgagg tagtcgcgaa tctattccag     1500

gtgccccaga atcccgtagt cgacgggatt cttgcttcac ctggggtact ccgcggtgca     1560

cacaacctcg actgcgtgtt aagagagggt gccacgctat tccctgtggt tattacgaca     1620

gtggaagacg ccatgacacc caaagcattg aacagcaaaa tgtttgctgt cattgaaggc     1680

gtgcgagaag acctccaacc tccatctcaa agaggatcct tcatacgaac tctctctgga     1740

cacagagtct atggatatgc tccagatggg gtacttccac tggagactgg gagagactac     1800

accgttgtcc caatagatga tgtctgggac gacagcatta tgctgtccaa agatcccata     1860

cctcctattg tgggaaacag tggaaatcta gccatagctt acatggatgt gtttcgaccc     1920

aaagtcccaa tccatgtggc tatgacggga gccctcaatg cttgtggcga gattgagaaa     1980

gtaagcttta gaagcaccaa gctcgctact gcacaccgac ttggccttag gttggctggt     2040

cccggagcat tcgatgtaaa caccgggccc aactgggcaa cgttcatcaa acgtttccct     2100

cacaatccac gcgactggga caggctcccc tacctcaacc taccatacct tccacccaat     2160

gcaggacgcc agtaccacct tgccatggct gcatcagagt tcaaagagac ccccgaactc     2220

gagagtgccg tcagagcaat ggaagcagca gccaacgtgg acccactatt ccaatctgca     2280

ctcagtgtgt tcatgtggct ggaagagaat gggattgtga ctgacatggc caacttcgca     2340

ctcagcgacc cgaacgccca tcggatgcga aattttcttg caaacgcacc acaagcaggc     2400

agcaagtcgc aaagggccaa gtacgggaca gcaggctacg gagtggaggc tcggggcccc     2460

acaccagagg aagcacagag ggaaaaagac acacggatct caaagaagat ggagaccatg     2520

ggcatctact ttgcaacacc agaatgggta gcactcaatg ggcaccgagg gccaagccca     2580

ggccaggtaa agtactggca gaacaaacga gaaataccgg acccaaacga ggactatcta     2640

gactacgtgc atg                                                        2653


<210>  12
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer used to amplify the GPT gene together with the
       E/L vaccinia promoter flanked by ClaI targets

<400>  12
gcgcatcgat catttcgcgg gatcgagatc c                                      31


<210>  13
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer used to amplify the GPT gene together with the
       E/L vaccinia promoter flanked by ClaI targets

<400>  13
gcgcatcgat agaaaaatta gcgaccggag attg                                   34


<210>  14
<211>  642
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence of the GPT gene preceded by the E/L vaccinia promoter
       flanked by ClaI targets

<400>  14
gcgcatcgat catttcgcgg gatcgagatc catgtcgatc gacgatcact aattccaaac       60

ccacccgctt tttatagtaa gtttttcacc cataaataat aaatacaata attaatttct      120

cgtaaaagta gaaaatatat tctaatttat tgcacggtaa ggaagtatga gcgaaaaata      180

catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc      240

ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac cgggtgcgtt      300

actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca      360

cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat      420

cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc      480

aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg gtgatgacta      540

tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt      600

cgtcccgcca atctccggtc gctaattttt ctatcgatgc gc                         642


<210>  15
<211>  8610
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pT-ReGS A plasmid sequence

<400>  15
cgatatatga attctaatac gactcactat aggatacgat cggtctgacc ccgggggagt       60

cacccgggga caggtcttca aggccttgtt ccaggatgga actcctcctt ctacaacgct      120

atcattgatg gtcagtagag atcagacaaa cgatcgcagc gatgacaaac ctgcaagatc      180

aaacccaaca gattgttccg ttcatacgga gccttctgat gccaacaacc ggaccggcgt      240

ccattccgga cgacaccctg gagaagcaca ctctcaggtc agagacctcg acctacaatt      300

tgactgtggg ggacacaggg tcagggctaa ttgtcttttt ccctggattc cctggctcaa      360

ttgtgggtgc tcactacaca ctgcagggca atgggaacta caagttcgat cagatgctcc      420

tgactgccca gaacctaccg gccagttaca actactgcag gctagtgagt cggagtctca      480

cagtgaggtc aagcacactt cctggtggcg tttatgcact aaacggcacc ataaacgccg      540

tgaccttcca aggaagcctg agtgaactga cagatgttag ctacaatggg ttgatgtctg      600

caacagccaa catcaacgac aaaattggga acgtcctagt aggggaaggg gtcaccgtcc      660

tcagcttacc cacatcatat gatcttgggt atgtgaggct tggtgacccc attcccgcaa      720

tagggcttga cccaaaaatg gtagccacat gtgacagcag tgacaggccc agagtctaca      780

ccataactgc agccgatgat taccaattct catcacagta ccaaccaggt ggggtaacaa      840

tcacactgtt ctcagccaac attgatgcca tcacaagcct cagcgttggg ggagagctcg      900

tgtttcgaac aagcgtccac ggccttgtac tgggcgccac catctacctc ataggctttg      960

atgggacaac ggtaatcacc agggctgtgg ccgcaaacaa tgggctgacg accggcaccg     1020

acaaccttat gccattcaat cttgtgattc caacaaacga gataacccag ccaatcacat     1080

ccatcaaact ggagatagtg acctccaaaa gtggtggtca ggcaggggat cagatgtcat     1140

ggtcggcaag agggagccta gcagtgacga tccatggtgg caactatcca ggggccctcc     1200

gtcccgtcac gctagtggcc tacgaaagag tggcaacagg atccgtcgtt acggtcgctg     1260

gggtgagcaa cttcgagctg atcccaaatc ctgaactagc aaagaacctg gttacagaat     1320

acggccgatt tgacccagga gccatgaact acacaaaatt gatactgagt gagagggacc     1380

gtcttggcat caagaccgtc tggccaacaa gggagtacac tgactttcgt gaatacttca     1440

tggaggtggc cgacctcaac tctcccctga agattgcagg agcattcggc ttcaaagaca     1500

taatccgggc cataaggagg atagctgtgc cggtggtctc cacattgttc ccacctgccg     1560

ctcccctagc ccatgcaatt ggggaaggtg tagactacct gctgggcgat gaggcccagg     1620

ccgcttcagg aactgctcga gccgcgtcag gaaaagcaag agctgcctca ggccgcataa     1680

ggcagctgac tctcgccgcc gacaaggggt acgaggtagt cgcgaatcta ttccaggtgc     1740

cccagaatcc cgtagtcgac gggattcttg cttcacctgg ggtactccgc ggtgcacaca     1800

acctcgactg cgtgttaaga gagggtgcca cgctattccc tgtggttatt acgacagtgg     1860

aagacgccat gacacccaaa gcattgaaca gcaaaatgtt tgctgtcatt gaaggcgtgc     1920

gagaagacct ccaacctcca tctcaaagag gatccttcat acgaactctc tctggacaca     1980

gagtctatgg atatgctcca gatggggtac ttccactgga gactgggaga gactacaccg     2040

ttgtcccaat agatgatgtc tgggacgaca gcattatgct gtccaaagat cccatacctc     2100

ctattgtggg aaacagtgga aatctagcca tagcttacat ggatgtgttt cgacccaaag     2160

tcccaatcca tgtggctatg acgggagccc tcaatgcttg tggcgagatt gagaaagtaa     2220

gctttagaag caccaagctc gctactgcac accgacttgg ccttaggttg gctggtcccg     2280

gagcattcga tgtaaacacc gggcccaact gggcaacgtt catcaaacgt ttccctcaca     2340

atccacgcga ctgggacagg ctcccctacc tcaacctacc ataccttcca cccaatgcag     2400

gacgccagta ccaccttgcc atggctgcat cagagttcaa agagaccccc gaactcgaga     2460

gtgccgtcag agcaatggaa gcagcagcca acgtggaccc actattccaa tctgcactca     2520

gtgtgttcat gtggctggaa gagaatggga ttgtgactga catggccaac ttcgcactca     2580

gcgacccgaa cgcccatcgg atgcgaaatt ttcttgcaaa cgcaccacaa gcaggcagca     2640

agtcgcaaag ggccaagtac gggacagcag gctacggagt ggaggctcgg ggccccacac     2700

cagaggaagc acagagggaa aaagacacac ggatctcaaa gaagatggag accatgggca     2760

tctactttgc aacaccagaa tgggtagcac tcaatgggca ccgagggcca agcccaggcc     2820

aggtaaagta ctggcagaac aaacgagaaa taccggaccc aaacgaggac tatctagact     2880

acgtgcatgc agagaagagc cggttggcat cagaagaaca aatcctaagg gcagctacgt     2940

cgatctacgg ggctccagga caggcagagc caccccaagc tttcatagac gaagttgcca     3000

aagtctatga aatcaaccat ggacgtggcc caaaccaaga acagatgaaa gatctgctct     3060

tgactgcgat ggagatgaag catcgcaatc ccaggcgggc tctaccaaag cccaagccaa     3120

aacccaatgc tccaacacag agacctcctg gtcggctggg ccgctggatc aggaccgtct     3180

ctgatgagga ccttgagtga ggctcctggg agtctcccga caccacccgc gcaggtgtgg     3240

acaccaattc ggccttacaa catcccaaat tggatccgtt cgcgggtccc ctgggtcggc     3300

atggcatctc cacctcctcg cggtccgacc tgggcatccg aaggaggacg tcgtccactc     3360

ggatggctaa gggagagctc ggatccggct gctaacaaag cccgaaagga agctgagttg     3420

gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg     3480

aggggttttt tgctgaaagg aggaactata tccggatcgc ggccgcccaa ttccgactcc     3540

ggaaccaatt actgataatg tagaagatca tacagacacc gtcacataca ctagtgatag     3600

cattaataca gtaagtgcat catctggaga atccacaaca gacgagactc cggaaccaat     3660

tactgataaa gaagatcata cagttacaga cactgtctca tacactacag taagtacatc     3720

atctggaatt gtcactacta aatcaaccac cgatgatgcg gatctttatg atacgtacaa     3780

tgataatgat acagtaccac caactactgt aggcggtagt acaacctcta ttagcaatta     3840

taaaaccaag gactttgtag aaatatttgg tattaccgca ttaattatat tgtcggccgt     3900

ggcaattttc tgtattacat attatatata taataaacgt tcacgtaaat acaaaacaga     3960

gaacaaagtc tagatttttg acttacataa atgtctggga tagtaaaatc tatcatattg     4020

agcggaccat ctggtttagg aaagacagcc atagccaaaa gactatggga atatatttgg     4080

atttgtggtg tcccatacca ctagatttcc tcgtcctatg gaacgagaag gtgttgatta     4140

ccattacgtt aacagagagg ccatctggaa gggaatagcc gccggaaact ttctagaaca     4200

tactgagttt ttaggaaata tttacggaac ttctaaaaca gctgtgaata cagcggctat     4260

taataatcgt atttgtgtga tggatctaaa catcgacggt gttagaagtc ttaaaaatac     4320

gtacctaatg ccttactcgg tgtatataag acctacctct cttaaaatgg ttgagaccaa     4380

gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac     4440

ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca     4500

ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt     4560

ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat     4620

ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc     4680

ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag     4740

ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt     4800

gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg     4860

cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa     4920

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa     4980

gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct     5040

tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg     5100

tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg     5160

ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt     5220

atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga     5280

cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga     5340

attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac     5400

gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg     5460

ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac     5520

gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct     5580

agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct     5640

gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg     5700

gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat     5760

ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg     5820

tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat     5880

tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct     5940

catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa     6000

gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa     6060

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc     6120

gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta     6180

gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct     6240

gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg     6300

atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag     6360

cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc     6420

cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg     6480

agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt     6540

tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg     6600

gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca     6660

catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg     6720

agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc     6780

ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag     6840

ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag     6900

ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg     6960

tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt     7020

tcgacgattg ttcatgatgg caagatttat atatctggag gttacaacaa tagtagtgta     7080

gttaatgtaa tatcgaatct agtccttagc tataatccga tatatgatga atggaccaaa     7140

ttatcatcat taaacattcc tagaattaat cccgctctat ggtcagcgca taataaatta     7200

tatgtaggag gaggaatatc tgatgatgtt cgaactaata catctgaaac atacgataaa     7260

gaaaaagatt gttggacatt ggataatggt cacgtgttac cacgcaatta tataatgtat     7320

aaatgcgaac cgattaaaca taaatatcca ttggaaaaaa cacagtacac gaatgatttt     7380

ctaaagtatt tggaaagttt tataggtagt tgatagaaca aaatacataa ttttgtaaaa     7440

ataaatcact ttttatacta atatgacacg attaccaata cttttgttac taatatcatt     7500

agtatacgct acaccttttc ctcagacatc taaaaaaata ggtgatgatg caactctatc     7560

atgtaatcga aataatacaa atgactacgt tgttatgagt gcttggtata aggagcccaa     7620

ttccattatt cttttagctg ctaaaagcga cgtcttgtat tttgataatt ataccaagga     7680

taaaatatct tacgactctc catacgatga tctagttaca actatcacaa ttaaatcatt     7740

gactgctaga gatgccggta cttatgtatg tgcattcttt atgacatcaa ctacaaatga     7800

cactgataaa gtagattatg aagaatactc cacagagttg attgtaaata cagatagtga     7860

atcgactata gacataatac tatctggatc tacacattca ccggaaacta gttctaagaa     7920

acctgattat atagataatt ctaattgctc gtcggtattc gaaatcggag ctgcggccgc     7980

atcgatagaa aaattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg     8040

gctgttcaat ccaggtatct tgcgggatat caacaacata gtcatcaccc agcggacgac     8100

cagccggttt tgcgaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa     8160

ccgcagtacc accggtatcc accaggtcat caataacgat gaagccttcg ccatcgcctt     8220

ctgcgcgttt cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa     8280

cggtatcgac atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc     8340

cacggcttac ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt     8400

tacgtgcatg gatctgcaac atgtcccagg tgacgatgta tttttcgctc atacttcctt     8460

accgtgcaat aaattagaat atattttcta cttttacgag aaattaatta ttgtatttat     8520

tatttatggg tgaaaaactt actataaaaa gcgggtgggt ttggaattag tgatcgtcga     8580

tcgacatgga tctcgatccc gcgaaatgat                                      8610


<210>  16
<211>  830
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Cas2 chimeric gene sequence

<400>  16
gcggccgcat cgatatatga attctaatac gactcactat aggatacgat gggtctgacc       60

ctctgggagt cacgaattaa cgtggctact aggggcgata cccgccgctg gccgccacgt      120

tagtggctcc tcttcttgat gattctgcca ccatgagtga cgttttcaat agtccacagg      180

cgcgaagcac gatctcagca gcgttcggca taaagcctac tgctggacaa gacgtggaag      240

aactcttgat ccctaaagtt tgggtgccac ctgaggatcc gcttgccagc cctagtcgac      300

ttccgtgtac acccccaagt acccagaagt caagaaccca cagaccgcct ccaaccccgt      360

tgttgggctc cacctgcccg ccaagagagc caccggtgtc caggccgctc ttctcggagc      420

aggaacgagc agaccaatgg ggatggaggc cccaacacgg tccaagaacg ccgtgaaaat      480

ggccaaacgg cggcaacgcc aaaaggagag ccgctaacag ccatgatggg aaccactcaa      540

gaagaggaca ctaatcccag accccgtatc cccggccttc gcctgcgggg gcccccgggt      600

cggcatggca tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc      660

actcggatgg ctaagggaga gctcggatcc ggctgctaac aaagcccgaa aggaagctga      720

gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt      780

cttgaggggt tttttgctga aaggaggaac tatatccgga tcgcggccgc                 830


<210>  17
<211>  3549
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pCas2 plasmid gene sequence

<400>  17
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tccaatgcgc gcggccgcat cgatatatga attctaatac gactcactat      480

aggatacgat gggtctgacc ctctgggagt cacgaattaa cgtggctact aggggcgata      540

cccgccgctg gccgccacgt tagtggctcc tcttcttgat gattctgcca ccatgagtga      600

cgttttcaat agtccacagg cgcgaagcac gatctcagca gcgttcggca taaagcctac      660

tgctggacaa gacgtggaag aactcttgat ccctaaagtt tgggtgccac ctgaggatcc      720

gcttgccagc cctagtcgac ttccgtgtac acccccaagt acccagaagt caagaaccca      780

cagaccgcct ccaaccccgt tgttgggctc cacctgcccg ccaagagagc caccggtgtc      840

caggccgctc ttctcggagc aggaacgagc agaccaatgg ggatggaggc cccaacacgg      900

tccaagaacg ccgtgaaaat ggccaaacgg cggcaacgcc aaaaggagag ccgctaacag      960

ccatgatggg aaccactcaa gaagaggaca ctaatcccag accccgtatc cccggccttc     1020

gcctgcgggg gcccccgggt cggcatggca tctccacctc ctcgcggtcc gacctgggca     1080

tccgaaggag gacgtcgtcc actcggatgg ctaagggaga gctcggatcc ggctgctaac     1140

aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc     1200

cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga     1260

tcgcggccgc atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt     1320

aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca     1380

tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat     1440

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt     1500

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct     1560

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     1620

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     1680

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     1740

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     1800

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     1860

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     1920

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     1980

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     2040

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     2100

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     2160

acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     2220

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     2280

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     2340

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     2400

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     2460

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     2520

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta     2580

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     2640

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     2700

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     2760

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt     2820

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     2880

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     2940

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     3000

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     3060

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg     3120

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     3180

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     3240

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     3300

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     3360

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     3420

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     3480

acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc     3540

cctttcgtc                                                             3549


<210>  18
<211>  5276
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pHA_Cas2 plasmid sequence

<400>  18
ggccgcatcg atatatgaat tctaatacga ctcactatag gatacgatgg gtctgaccct       60

ctgggagtca cgaattaacg tggctactag gggcgatacc cgccgctggc cgccacgtta      120

gtggctcctc ttcttgatga ttctgccacc atgagtgacg ttttcaatag tccacaggcg      180

cgaagcacga tctcagcagc gttcggcata aagcctactg ctggacaaga cgtggaagaa      240

ctcttgatcc ctaaagtttg ggtgccacct gaggatccgc ttgccagccc tagtcgactt      300

ccgtgtacac ccccaagtac ccagaagtca agaacccaca gaccgcctcc aaccccgttg      360

ttgggctcca cctgcccgcc aagagagcca ccggtgtcca ggccgctctt ctcggagcag      420

gaacgagcag accaatgggg atggaggccc caacacggtc caagaacgcc gtgaaaatgg      480

ccaaacggcg gcaacgccaa aaggagagcc gctaacagcc atgatgggaa ccactcaaga      540

agaggacact aatcccagac cccgtatccc cggccttcgc ctgcgggggc ccccgggtcg      600

gcatggcatc tccacctcct cgcggtccga cctgggcatc cgaaggagga cgtcgtccac      660

tcggatggct aagggagagc tcggatccgg ctgctaacaa agcccgaaag gaagctgagt      720

tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct      780

tgaggggttt tttgctgaaa ggaggaacta tatccggatc gcggccgcag ctccgatttc      840

gaataccgac gagcaattag aattatctat ataatcaggt ttcttagaac tagtttccgg      900

tgaatgtgta gatccagata gtattatgtc tatagtcgat tcactatctg tatttacaat      960

caactctgtg gagtattctt cataatctac tttatcagtg tcatttgtag ttgatgtcat     1020

aaagaatgca catacataag taccggcatc tctagcagtc aatgatttaa ttgtgatagt     1080

tgtaactaga tcatcgtatg gagagtcgta agatatttta tccttggtat aattatcaaa     1140

atacaagacg tcgcttttag cagctaaaag aataatggaa ttgggctcct tataccaagc     1200

actcataaca acgtagtcat ttgtattatt tcgattacat gatagagttg catcatcacc     1260

tattttttta gatgtctgag gaaaaggtgt agcgtatact aatgatatta gtaacaaaag     1320

tattggtaat cgtgtcatat tagtataaaa agtgatttat ttttacaaaa ttatgtattt     1380

tgttctatca actacctata aaactttcca aatactttag aaaatcattc gtgtactgtg     1440

ttttttccaa tggatattta tgtttaatcg gttcgcattt atacattata taattgcgtg     1500

gtaacacgtg accattatcc aatgtccaac aatctttttc tttatcgtat gtttcagatg     1560

tattagttcg aacatcatca gatattcctc ctcctacata taatttatta tgcgctgacc     1620

atagagcggg attaattcta ggaatgttta atgatgataa tttggtccat tcatcatata     1680

tcggattata gctaaggact agattcgata ttacattaac tacactacta ttgttgtaac     1740

ctccagatat ataaatcttg ccatcatgaa caatcgtcga aattcgtaat catggtcata     1800

gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag     1860

cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg     1920

ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca     1980

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     2040

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     2100

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     2160

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     2220

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     2280

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     2340

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     2400

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     2460

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     2520

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     2580

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     2640

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     2700

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     2760

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     2820

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     2880

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     2940

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     3000

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     3060

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     3120

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     3180

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     3240

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     3300

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     3360

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     3420

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     3480

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     3540

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     3600

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     3660

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     3720

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     3780

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     3840

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     3900

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac     3960

cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc     4020

gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc     4080

ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg     4140

cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca     4200

tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat     4260

tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta     4320

cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt     4380

tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttggtctcaa ccattttaag     4440

agaggtaggt cttatataca ccgagtaagg cattaggtac gtatttttaa gacttctaac     4500

accgtcgatg tttagatcca tcacacaaat acgattatta atagccgctg tattcacagc     4560

tgttttagaa gttccgtaaa tatttcctaa aaactcagta tgttctagaa agtttccggc     4620

ggctattccc ttccagatgg cctctctgtt aacgtaatgg taatcaacac cttctcgttc     4680

cataggacga ggaaatctag tggtatggga caccacaaat ccaaatatat tcccatagtc     4740

ttttggctat ggctgtcttt cctaaaccag atggtccgct caatatgata gattttacta     4800

tcccagacat ttatgtaagt caaaaatcta gactttgttc tctgttttgt atttacgtga     4860

acgtttatta tatatataat atgtaataca gaaaattgcc acggccgaca atataattaa     4920

tgcggtaata ccaaatattt ctacaaagtc cttggtttta taattgctaa tagaggttgt     4980

actaccgcct acagtagttg gtggtactgt atcattatca ttgtacgtat cataaagatc     5040

cgcatcatcg gtggttgatt tagtagtgac aattccagat gatgtactta ctgtagtgta     5100

tgagacagtg tctgtaactg tatgatcttc tttatcagta attggttccg gagtctcgtc     5160

tgttgtggat tctccagatg atgcacttac tgtattaatg ctatcactag tgtatgtgac     5220

ggtgtctgta tgatcttcta cattatcagt aattggttcc ggagtcggaa ttgggc         5276


<210>  19
<211>  2272
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence extracted from the pFastBac-VP1 plasmid by restriction
       with the Sal I enzyme.

<400>  19
tcgactggca aagttcctca gagagaacgg ctacaaagtt ttgcagccgc ggtctctgcc       60

cgagaatgag gagtatgaga ccgaccaaat actcccagac ttagcatgga tgcgacagat      120

agaaggggct gttttaaaac ccactctatc tctccctatt ggagatcagg agtacttccc      180

aaagtactac ccaacacatc gccctagcaa ggagaagccc aatgcgtacc caccagacat      240

cgcactactc aagcagatga tttacctgtt tctccaggtt ccagaggcca acgagggcct      300

aaaggatgaa gtaaccctct tgacccaaaa cataagggac aaggcctatg gaagtgggac      360

ctacatggga caagcaaatc gacttgtggc catgaaggag gtcgccactg gaagaaaccc      420

aaacaaggat cctctaaagc ttgggtacac ttttgagagc atcgcgcagc tacttgacat      480

cacactaccg gtaggcccac ccggtgagga tgacaagccc tgggtgccac tcacaagagt      540

gccgtcacgg atgttggtgc tgacgggaga cgtagatggc gactttgagg ttgaagatta      600

ccttcccaaa atcaacctca agtcatcaag tggactacca tatgtaggtc gcaccaaagg      660

agagacaatt ggcgagatga tagctatatc aaaccagttt ctcagagagc tatcaacact      720

gttgaagcaa ggtgcaggga caaaggggtc aaacaagaag aagctactca gcatgttaag      780

tgactattgg tacttatcat gcgggctttt gtttccaaag gctgaaaggt acgacaaaag      840

tacatggctc accaagaccc ggaacatatg gtcagctcca tccccaacac acctcatgat      900

ctccatgatc acctggcccg tgatgtccaa cagcccaaat aacgtgttga acattgaagg      960

gtgtccatca ctctacaaat tcaacccgtt cagaggaggg ttgaacagga tcgtcgagtg     1020

gatattggcc ccggaagaac ccaaggctct tgtatatgcg gacaacatat acattgtcca     1080

ctcaaacacg tggtactcaa ttgacctaga gaagggtgag gcaaactgca ctcgccaaca     1140

catgcaagcc gcaatgtact acatactcac cagagggtgg tcagacaacg gcgacccaat     1200

gttcaatcaa acatgggcca cctttgccat gaacattgcc cctgctctag tggtggactc     1260

atcgtgcctg ataatgaacc tgcaaattaa gacctatggt caaggcagcg ggaatgcagc     1320

cacgttcatc aacaaccacc tcttgagcac gctagtgctt gaccagtgga acttgatgag     1380

acagcccaga ccagacagcg aggagttcaa atcaattgag gacaagctag gtatcaactt     1440

taagattgag aggtccattg atgatatcag gggcaagctg agacagcttg tcctccttgc     1500

acaaccaggg tacctgagtg ggggggttga accagaacaa tccagcccaa ctgttgagct     1560

tgacctacta gggtggtcag ctacatacag caaagatctc gggatctatg tgccggtgct     1620

tgacaaggaa cgcctatttt gttctgctgc gtatcccaag ggagtagaga acaagagtct     1680

caagtccaaa gtcgggatcg agcaggcata caaggtagtc aggtatgagg cgttaaggtt     1740

ggtaggtggt tggaactacc cactcctgaa caaagcctgc aagaataacg caggcgccgc     1800

tcggcggcat ctggaggcca aggggttccc actcgacgag ttcctagccg agtggtctga     1860

gctgtcagag ttcggtgagg ccttcgaagg cttcaatatc aagctgaccg taacatctga     1920

gagcctagcc gaactgaaca agccagtacc ccccaagccc ccaaatgtca acagaccagt     1980

caacactggg ggactcaagg cagtcagcaa cgccctcaag accggtcggt acaggaacga     2040

agccggactg agtggtctcg tccttctagc cacagcaaga agccgtctgc aagatgcagt     2100

taaggccaag gcagaagccg agaaactcca caagtccaag ccagacgacc ccgatgcaga     2160

ctggttcgaa agatcagaaa ctctgtcaga ccttctggag aaagccgaca tcgccagcaa     2220

ggtcgcccac tcagcactcg tggaaacaag cgacgccctt gaagcagttc ag             2272


<210>  20
<211>  8176
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pT-ReGS-B plasmid sequence

<400>  20
cgatatatga attctaatac gactcactat aggatacgat gggtctgacc ctctgggagt       60

cacgaattaa cgtggctact aggggcgata cccgccgctg gccgccacgt tagtggctcc      120

tcttcttgat gattctgcca ccatgagtga cgttttcaat agtccacagg cgcgaagcac      180

gatctcagca gcgttcggca taaagcctac tgctggacaa gacgtggaag aactcttgat      240

ccctaaagtt tgggtgccac ctgaggatcc gcttgccagc cctagtcgac tggcaaagtt      300

cctcagagag aacggctaca aagttttgca gccgcggtct ctgcccgaga atgaggagta      360

tgagaccgac caaatactcc cagacttagc atggatgcga cagatagaag gggctgtttt      420

aaaacccact ctatctctcc ctattggaga tcaggagtac ttcccaaagt actacccaac      480

acatcgccct agcaaggaga agcccaatgc gtacccacca gacatcgcac tactcaagca      540

gatgatttac ctgtttctcc aggttccaga ggccaacgag ggcctaaagg atgaagtaac      600

cctcttgacc caaaacataa gggacaaggc ctatggaagt gggacctaca tgggacaagc      660

aaatcgactt gtggccatga aggaggtcgc cactggaaga aacccaaaca aggatcctct      720

aaagcttggg tacacttttg agagcatcgc gcagctactt gacatcacac taccggtagg      780

cccacccggt gaggatgaca agccctgggt gccactcaca agagtgccgt cacggatgtt      840

ggtgctgacg ggagacgtag atggcgactt tgaggttgaa gattaccttc ccaaaatcaa      900

cctcaagtca tcaagtggac taccatatgt aggtcgcacc aaaggagaga caattggcga      960

gatgatagct atatcaaacc agtttctcag agagctatca acactgttga agcaaggtgc     1020

agggacaaag gggtcaaaca agaagaagct actcagcatg ttaagtgact attggtactt     1080

atcatgcggg cttttgtttc caaaggctga aaggtacgac aaaagtacat ggctcaccaa     1140

gacccggaac atatggtcag ctccatcccc aacacacctc atgatctcca tgatcacctg     1200

gcccgtgatg tccaacagcc caaataacgt gttgaacatt gaagggtgtc catcactcta     1260

caaattcaac ccgttcagag gagggttgaa caggatcgtc gagtggatat tggccccgga     1320

agaacccaag gctcttgtat atgcggacaa catatacatt gtccactcaa acacgtggta     1380

ctcaattgac ctagagaagg gtgaggcaaa ctgcactcgc caacacatgc aagccgcaat     1440

gtactacata ctcaccagag ggtggtcaga caacggcgac ccaatgttca atcaaacatg     1500

ggccaccttt gccatgaaca ttgcccctgc tctagtggtg gactcatcgt gcctgataat     1560

gaacctgcaa attaagacct atggtcaagg cagcgggaat gcagccacgt tcatcaacaa     1620

ccacctcttg agcacgctag tgcttgacca gtggaacttg atgagacagc ccagaccaga     1680

cagcgaggag ttcaaatcaa ttgaggacaa gctaggtatc aactttaaga ttgagaggtc     1740

cattgatgat atcaggggca agctgagaca gcttgtcctc cttgcacaac cagggtacct     1800

gagtgggggg gttgaaccag aacaatccag cccaactgtt gagcttgacc tactagggtg     1860

gtcagctaca tacagcaaag atctcgggat ctatgtgccg gtgcttgaca aggaacgcct     1920

attttgttct gctgcgtatc ccaagggagt agagaacaag agtctcaagt ccaaagtcgg     1980

gatcgagcag gcatacaagg tagtcaggta tgaggcgtta aggttggtag gtggttggaa     2040

ctacccactc ctgaacaaag cctgcaagaa taacgcaggc gccgctcggc ggcatctgga     2100

ggccaagggg ttcccactcg acgagttcct agccgagtgg tctgagctgt cagagttcgg     2160

tgaggccttc gaaggcttca atatcaagct gaccgtaaca tctgagagcc tagccgaact     2220

gaacaagcca gtacccccca agcccccaaa tgtcaacaga ccagtcaaca ctgggggact     2280

caaggcagtc agcaacgccc tcaagaccgg tcggtacagg aacgaagccg gactgagtgg     2340

tctcgtcctt ctagccacag caagaagccg tctgcaagat gcagttaagg ccaaggcaga     2400

agccgagaaa ctccacaagt ccaagccaga cgaccccgat gcagactggt tcgaaagatc     2460

agaaactctg tcagaccttc tggagaaagc cgacatcgcc agcaaggtcg cccactcagc     2520

actcgtggaa acaagcgacg cccttgaagc agttcagtcg acttccgtgt acacccccaa     2580

gtacccagaa gtcaagaacc cacagaccgc ctccaacccc gttgttgggc tccacctgcc     2640

cgccaagaga gccaccggtg tccaggccgc tcttctcgga gcaggaacga gcagaccaat     2700

ggggatggag gccccaacac ggtccaagaa cgccgtgaaa atggccaaac ggcggcaacg     2760

ccaaaaggag agccgctaac agccatgatg ggaaccactc aagaagagga cactaatccc     2820

agaccccgta tccccggcct tcgcctgcgg gggcccccgg gtcggcatgg catctccacc     2880

tcctcgcggt ccgacctggg catccgaagg aggacgtcgt ccactcggat ggctaaggga     2940

gagctcggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc     3000

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct     3060

gaaaggagga actatatccg gatcgcggcc gcagctccga tttcgaatac cgacgagcaa     3120

ttagaattat ctatataatc aggtttctta gaactagttt ccggtgaatg tgtagatcca     3180

gatagtatta tgtctatagt cgattcacta tctgtattta caatcaactc tgtggagtat     3240

tcttcataat ctactttatc agtgtcattt gtagttgatg tcataaagaa tgcacataca     3300

taagtaccgg catctctagc agtcaatgat ttaattgtga tagttgtaac tagatcatcg     3360

tatggagagt cgtaagatat tttatccttg gtataattat caaaatacaa gacgtcgctt     3420

ttagcagcta aaagaataat ggaattgggc tccttatacc aagcactcat aacaacgtag     3480

tcatttgtat tatttcgatt acatgataga gttgcatcat cacctatttt tttagatgtc     3540

tgaggaaaag gtgtagcgta tactaatgat attagtaaca aaagtattgg taatcgtgtc     3600

atattagtat aaaaagtgat ttatttttac aaaattatgt attttgttct atcaactacc     3660

tataaaactt tccaaatact ttagaaaatc attcgtgtac tgtgtttttt ccaatggata     3720

tttatgttta atcggttcgc atttatacat tatataattg cgtggtaaca cgtgaccatt     3780

atccaatgtc caacaatctt tttctttatc gtatgtttca gatgtattag ttcgaacatc     3840

atcagatatt cctcctccta catataattt attatgcgct gaccatagag cgggattaat     3900

tctaggaatg tttaatgatg ataatttggt ccattcatca tatatcggat tatagctaag     3960

gactagattc gatattacat taactacact actattgttg taacctccag atatataaat     4020

cttgccatca tgaacaatcg tcgaaattcg taatcatggt catagctgtt tcctgtgtga     4080

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc     4140

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     4200

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     4260

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     4320

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     4380

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     4440

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     4500

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     4560

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     4620

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     4680

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     4740

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     4800

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     4860

gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc     4920

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     4980

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     5040

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     5100

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     5160

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     5220

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     5280

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     5340

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     5400

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     5460

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     5520

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     5580

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     5640

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     5700

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     5760

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     5820

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     5880

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     5940

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     6000

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     6060

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     6120

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     6180

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     6240

ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac     6300

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat     6360

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg     6420

cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa     6480

taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc     6540

gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag     6600

ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt     6660

gtaaaacgac ggccagtgcc aagcttggtc tcaaccattt taagagaggt aggtcttata     6720

tacaccgagt aaggcattag gtacgtattt ttaagacttc taacaccgtc gatgtttaga     6780

tccatcacac aaatacgatt attaatagcc gctgtattca cagctgtttt agaagttccg     6840

taaatatttc ctaaaaactc agtatgttct agaaagtttc cggcggctat tcccttccag     6900

atggcctctc tgttaacgta atggtaatca acaccttctc gttccatagg acgaggaaat     6960

ctagtggtat gggacaccac aaatccaaat atattcccat agtcttttgg ctatggctgt     7020

ctttcctaaa ccagatggtc cgctcaatat gatagatttt actatcccag acatttatgt     7080

aagtcaaaaa tctagacttt gttctctgtt ttgtatttac gtgaacgttt attatatata     7140

taatatgtaa tacagaaaat tgccacggcc gacaatataa ttaatgcggt aataccaaat     7200

atttctacaa agtccttggt tttataattg ctaatagagg ttgtactacc gcctacagta     7260

gttggtggta ctgtatcatt atcattgtac gtatcataaa gatccgcatc atcggtggtt     7320

gatttagtag tgacaattcc agatgatgta cttactgtag tgtatgagac agtgtctgta     7380

actgtatgat cttctttatc agtaattggt tccggagtct cgtctgttgt ggattctcca     7440

gatgatgcac ttactgtatt aatgctatca ctagtgtatg tgacggtgtc tgtatgatct     7500

tctacattat cagtaattgg ttccggagtc ggaattgggc ggccgcatcg atagaaaaat     7560

tagcgaccgg agattggcgg gacgaatacg acgcccatat cccacggctg ttcaatccag     7620

gtatcttgcg ggatatcaac aacatagtca tcacccagcg gacgaccagc cggttttgcg     7680

aagatggtga caaagtgcgc ttttggatac atttcacgaa tcgcaaccgc agtaccaccg     7740

gtatccacca ggtcatcaat aacgatgaag ccttcgccat cgccttctgc gcgtttcagc     7800

actttaagct cgcgctggtt gtcgtgatcg tagctggaaa tacaaacggt atcgacatga     7860

cgaataccca gttcacgcgc cagtaacgca cccggtacca gaccgccacg gcttacggca     7920

ataatgcctt tccattgttc agaaggcatc agtcggcttg cgagtttacg tgcatggatc     7980

tgcaacatgt cccaggtgac gatgtatttt tcgctcatac ttccttaccg tgcaataaat     8040

tagaatatat tttctacttt tacgagaaat taattattgt atttattatt tatgggtgaa     8100

aaacttacta taaaaagcgg gtgggtttgg aattagtgat cgtcgatcga catggatctc     8160

gatcccgcga aatgat                                                     8176


<210>  21
<211>  266
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GFP-IBDV polyprotein fusion protein 

<400>  21

Met Thr Asn Leu Gln Asp Gln Thr Gln Gln Ile Val Pro Phe Ile Arg 
1               5                   10                  15      


Ser Leu Leu Met Pro Thr Thr Gly Pro Gly Met Ser Lys Gly Glu Glu 
            20                  25                  30          


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
        35                  40                  45              


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
    50                  55                  60                  


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
65                  70                  75                  80  


Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys 
                85                  90                  95      


Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
            100                 105                 110         


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
        115                 120                 125             


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
    130                 135                 140                 


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
145                 150                 155                 160 


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
                165                 170                 175     


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
            180                 185                 190         


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
        195                 200                 205             


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
    210                 215                 220                 


His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
225                 230                 235                 240 


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
                245                 250                 255     


Leu Gly Met Asp Glu Leu Tyr Lys Ser Gly 
            260                 265     


<210>  22
<211>  282
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IBDV ORF1A-ORF2 fusion protein

<400>  22

Met Val Ser Arg Asp Gln Thr Asn Asp Arg Ser Asp Asp Lys Pro Ala 
1               5                   10                  15      


Arg Ser Asn Pro Thr Asp Cys Ser Val His Thr Glu Pro Ser Asp Ala 
            20                  25                  30          


Asn Asn Arg Thr Gly Asp Glu Gln Gly Arg Gly Ala Val His Arg Gly 
        35                  40                  45              


Gly Ala His Pro Gly Arg Ala Gly Arg Arg Arg Lys Arg Pro Gln Val 
    50                  55                  60                  


Gln Arg Val Arg Arg Gly Arg Gly Arg Cys His Leu Arg Gln Ala Asp 
65                  70                  75                  80  


Pro Glu Val His Leu His His Arg Gln Ala Ala Arg Ala Leu Ala His 
                85                  90                  95      


Pro Arg Asp His Pro Asp Leu Arg Arg Ala Val Leu Gln Pro Leu Pro 
            100                 105                 110         


Arg Pro His Glu Ala Ala Arg Leu Leu Gln Val Arg His Ala Arg Arg 
        115                 120                 125             


Leu Arg Pro Gly Ala His His Leu Leu Gln Gly Arg Arg Gln Leu Gln 
    130                 135                 140                 


Asp Pro Arg Arg Gly Glu Val Arg Gly Arg His Pro Gly Glu Pro His 
145                 150                 155                 160 


Arg Ala Glu Gly His Arg Leu Gln Gly Gly Arg Gln His Pro Gly Ala 
                165                 170                 175     


Gln Ala Gly Val Gln Leu Gln Gln Pro Gln Arg Leu Tyr His Gly Arg 
            180                 185                 190         


Gln Ala Glu Glu Arg His Gln Gly Glu Leu Gln Asp Pro Pro Gln His 
        195                 200                 205             


Arg Gly Arg Gln Arg Ala Ala Arg Arg Pro Leu Pro Ala Glu His Pro 
    210                 215                 220                 


His Arg Arg Arg Pro Arg Ala Ala Ala Arg Gln Pro Leu Pro Glu His 
225                 230                 235                 240 


Pro Val Arg Pro Glu Gln Arg Pro Gln Arg Glu Ala Arg Ser His Gly 
                245                 250                 255     


Pro Ala Gly Val Arg Asp Arg Arg Arg Asp His Ser Arg His Gly Arg 
            260                 265                 270         


Ala Val Gln Val Arg Ile Lys Ile Cys Ser 
        275                 280         


<210>  23
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Forward primer to amplify the sequence encoding EGFP from
       pEGFP-C1

<400>  23
cgcgcccggg atgagcaagg gcgaggagct g                                      31


<210>  24
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Reverse primer to amplify EGFP from pEGFP-C1

<400>  24
gcgcagatct gattatccgg acttgtacag ctcgtc                                 36


<210>  25
<211>  739
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence encoding EGFP flanked by SmaI and BglII sites

<400>  25
cgcgcccggg atgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga       60

gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc      120

cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg      180

gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca      240

catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac      300

catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga      360

caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct      420

ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca      480

gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca      540

gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga      600

caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca      660

catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta      720

caagtccgga taaagatct                                                   739


<210>  26
<211>  6523
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pT-ReGS-AGFP plasmid sequence

<400>  26
cgatatatga attctaatac gactcactat aggatacgat cggtctgacc ccgggggagt       60

cacccgggga caggtcttca aggccttgtt ccaggatgga actcctcctt ctacaacgct      120

atcattgatg gtcagtagag atcagacaaa cgatcgcagc gatgacaaac ctgcaagatc      180

aaacccaaca gattgttccg ttcatacgga gccttctgat gccaacaacc ggaccgggga      240

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg      300

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca      360

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg      420

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc      480

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca      540

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga      600

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc      660

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca      720

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc      780

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc      840

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc      900

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggat      960

aaagatctgc tcttgactgc gatggagatg aagcatcgca atcccaggcg ggctctacca     1020

aagcccaagc caaaacccaa tgctccaaca cagagacctc ctggtcggct gggccgctgg     1080

atcaggaccg tctctgatga ggaccttgag tgaggctcct gggagtctcc cgacaccacc     1140

cgcgcaggtg tggacaccaa ttcggcctta caacatccca aattggatcc gttcgcgggt     1200

cccctgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat ccgaaggagg     1260

acgtcgtcca ctcggatggc taagggagag ctcggatccg gctgctaaca aagcccgaaa     1320

ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc     1380

taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat cgcggccgcc     1440

caattccgac tccggaacca attactgata atgtagaaga tcatacagac accgtcacat     1500

acactagtga tagcattaat acagtaagtg catcatctgg agaatccaca acagacgaga     1560

ctccggaacc aattactgat aaagaagatc atacagttac agacactgtc tcatacacta     1620

cagtaagtac atcatctgga attgtcacta ctaaatcaac caccgatgat gcggatcttt     1680

atgatacgta caatgataat gatacagtac caccaactac tgtaggcggt agtacaacct     1740

ctattagcaa ttataaaacc aaggactttg tagaaatatt tggtattacc gcattaatta     1800

tattgtcggc cgtggcaatt ttctgtatta catattatat atataataaa cgttcacgta     1860

aatacaaaac agagaacaaa gtctagattt ttgacttaca taaatgtctg ggatagtaaa     1920

atctatcata ttgagcggac catctggttt aggaaagaca gccatagcca aaagactatg     1980

ggaatatatt tggatttgtg gtgtcccata ccactagatt tcctcgtcct atggaacgag     2040

aaggtgttga ttaccattac gttaacagag aggccatctg gaagggaata gccgccggaa     2100

actttctaga acatactgag tttttaggaa atatttacgg aacttctaaa acagctgtga     2160

atacagcggc tattaataat cgtatttgtg tgatggatct aaacatcgac ggtgttagaa     2220

gtcttaaaaa tacgtaccta atgccttact cggtgtatat aagacctacc tctcttaaaa     2280

tggttgagac caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct     2340

ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc     2400

gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc     2460

ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca     2520

ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac     2580

ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga     2640

ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac     2700

gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt     2760

agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     2820

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     2880

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg     2940

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg     3000

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc     3060

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat     3120

gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact     3180

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca     3240

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact     3300

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg     3360

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg     3420

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg     3480

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg     3540

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag     3600

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc     3660

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga     3720

tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat     3780

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     3840

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     3900

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     3960

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     4020

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc     4080

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     4140

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     4200

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     4260

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     4320

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     4380

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     4440

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     4500

ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct     4560

ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta     4620

ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag     4680

tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     4740

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg     4800

caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg     4860

ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc     4920

atgattacga atttcgacga ttgttcatga tggcaagatt tatatatctg gaggttacaa     4980

caatagtagt gtagttaatg taatatcgaa tctagtcctt agctataatc cgatatatga     5040

tgaatggacc aaattatcat cattaaacat tcctagaatt aatcccgctc tatggtcagc     5100

gcataataaa ttatatgtag gaggaggaat atctgatgat gttcgaacta atacatctga     5160

aacatacgat aaagaaaaag attgttggac attggataat ggtcacgtgt taccacgcaa     5220

ttatataatg tataaatgcg aaccgattaa acataaatat ccattggaaa aaacacagta     5280

cacgaatgat tttctaaagt atttggaaag ttttataggt agttgataga acaaaataca     5340

taattttgta aaaataaatc actttttata ctaatatgac acgattacca atacttttgt     5400

tactaatatc attagtatac gctacacctt ttcctcagac atctaaaaaa ataggtgatg     5460

atgcaactct atcatgtaat cgaaataata caaatgacta cgttgttatg agtgcttggt     5520

ataaggagcc caattccatt attcttttag ctgctaaaag cgacgtcttg tattttgata     5580

attataccaa ggataaaata tcttacgact ctccatacga tgatctagtt acaactatca     5640

caattaaatc attgactgct agagatgccg gtacttatgt atgtgcattc tttatgacat     5700

caactacaaa tgacactgat aaagtagatt atgaagaata ctccacagag ttgattgtaa     5760

atacagatag tgaatcgact atagacataa tactatctgg atctacacat tcaccggaaa     5820

ctagttctaa gaaacctgat tatatagata attctaattg ctcgtcggta ttcgaaatcg     5880

gagctgcggc cgcatcgata gaaaaattag cgaccggaga ttggcgggac gaatacgacg     5940

cccatatccc acggctgttc aatccaggta tcttgcggga tatcaacaac atagtcatca     6000

cccagcggac gaccagccgg ttttgcgaag atggtgacaa agtgcgcttt tggatacatt     6060

tcacgaatcg caaccgcagt accaccggta tccaccaggt catcaataac gatgaagcct     6120

tcgccatcgc cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag     6180

ctggaaatac aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc     6240

ggtaccagac cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt     6300

cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg     6360

ctcatacttc cttaccgtgc aataaattag aatatatttt ctacttttac gagaaattaa     6420

ttattgtatt tattatttat gggtgaaaaa cttactataa aaagcgggtg ggtttggaat     6480

tagtgatcgt cgatcgacat ggatctcgat cccgcgaaat gat                       6523


