                         SEQUENCE LISTING

<110>  United Biomedical Inc
       Wang, Chang Yi
       Wen-Jiun, Peng
 
<120>  Designer Peptide-Based PCV2 Vaccine

<130>  1004263.204WO (2029-WO)

<140>  TBD
<141>  2010-07-08

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  154
<212>  PRT
<213>  Porcine circovirus - type 2 strain Cyc08 from Taiwan

<400>  1

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Ala Thr Thr Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Ser Asp 
            20                  25                  30          


Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ser 
    130                 135                 140                 


Ala Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  2
<211>  19
<212>  PRT
<213>  Measles virus - Modified T helper site


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  S or T

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  K or R

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  G or T

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  H or T

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  K or R

<400>  2

Ile Ser Ile Xaa Glu Ile Xaa Xaa Val Ile Val Xaa Xaa Ile Glu Thr 
1               5                   10                  15      


Ile Leu Phe 
            


<210>  3
<211>  20
<212>  PRT
<213>  Porcine circovirus

<400>  3

Cys His Ile Glu Lys Ala Lys Gly Thr Asp Gln Gln Asn Lys Glu Tyr 
1               5                   10                  15      


Cys Ser Lys Glu 
            20  


<210>  4
<211>  20
<212>  PRT
<213>  Porcine circovirus

<400>  4

Lys Trp Trp Asp Gly Tyr His Gly Glu Glu Val Val Val Ile Asp Asp 
1               5                   10                  15      


Phe Tyr Gly Trp 
            20  


<210>  5
<211>  20
<212>  PRT
<213>  Porcine circovirus

<400>  5

Pro Arg Trp Pro His Asn Asp Val Tyr Ile Gly Leu Pro Ile Thr Leu 
1               5                   10                  15      


Leu His Phe Pro 
            20  


<210>  6
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B cell epitope cluster peptide derived from PCV2 ORF2 capsid 
       protein (Cap1 peptide) linked through spacer to artificial 
       combinatorial Th peptide (UBITh3)


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  S or T

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  K or R

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  G or T

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  H or T

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  K or R

<220>
<221>  MOD_RES
<222>  (23)..(23)
<223>  AMIDATION

<220>
<221>  MISC_FEATURE
<222>  (23)..(23)
<223>  (epsilonN)Lys

<400>  6

Ile Ser Ile Xaa Glu Ile Xaa Xaa Val Ile Val Xaa Xaa Ile Glu Thr 
1               5                   10                  15      


Ile Leu Phe Lys Lys Lys Xaa Thr Arg Leu Ser Arg Thr Phe Gly Tyr 
            20                  25                  30          


Thr Val Lys Ala Thr Thr Val Arg Thr Pro Ser Trp Ala Val Asp Met 
        35                  40                  45              


Met Arg Phe Asn Ile Ser Asp Phe Val Pro Pro Gly Gly Gly Thr Asn 
    50                  55                  60                  


Lys Ile Ser Ile Pro Phe Glu Tyr Tyr Arg Ile Arg Lys Val Lys Val 
65                  70                  75                  80  


Glu Phe Trp Pro Cys Ser Pro Ile Thr Gln Gly Asp Arg Gly Val Gly 
                85                  90                  95      


Ser Thr Ala Val Ile Leu Asp Asp Asn Phe Val Thr Lys Ala Thr Ala 
            100                 105                 110         


Leu Thr Tyr Asp Pro Tyr Val Asn Tyr Ser Ser Arg His Thr Ile Pro 
        115                 120                 125             


Gln Pro Phe Ser Tyr His Ser Arg Tyr Phe Thr Pro Lys Pro Val Leu 
    130                 135                 140                 


Asp Ser Thr Ile Asp Tyr Phe Gln Pro Asn Asn Lys Arg Asn Gln Leu 
145                 150                 155                 160 


Trp Leu Arg Leu Gln Thr Ser Ala Asn Val Asp His Val Gly Leu Gly 
                165                 170                 175     


Thr 
    


<210>  7
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  artificial spacer sequence


<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  AMIDATION

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  (epsilonN)Lys

<400>  7

Lys Lys Lys Xaa 
1               


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG oligo nucleotide

<400>  8
tcgtcgtttt gtcgttttgt cgtt                                              24


<210>  9
<211>  154
<212>  PRT
<213>  Porcine circovirus - US

<400>  9

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Ile Lys Arg Thr Thr Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Leu Pro Pro Gly Gly Gly Ser Asn Pro Arg Ser Val Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Ser Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ala 
    130                 135                 140                 


Gly Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  10
<211>  154
<212>  PRT
<213>  Porcine circovirus - DK

<400>  10

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Ile Lys Arg Thr Thr Val 
1               5                   10                  15      


Lys Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Leu Pro Pro Gly Gly Gly Ser Asn Pro Arg Ser Val Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Ser Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ala 
    130                 135                 140                 


Gly Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  11
<211>  154
<212>  PRT
<213>  Porcine circovirus - DE

<400>  11

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Ile Lys Arg Thr Thr Val 
1               5                   10                  15      


Lys Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Leu Pro Pro Gly Gly Gly Ser Asn Pro Arg Ser Val Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Ser Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Met Arg Leu Gln Thr Ser 
    130                 135                 140                 


Arg Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  12
<211>  150
<212>  PRT
<213>  Porcine circovirus - ES

<400>  12

Arg Thr Phe Gly Tyr Thr Val Lys Arg Thr Pro Val Arg Thr Pro Ser 
1               5                   10                  15      


Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp Phe Leu Pro Pro 
            20                  25                  30          


Gly Gly Gly Ser Asn His Arg Ser Val Pro Phe Glu Tyr Tyr Arg Ile 
        35                  40                  45              


Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro Ile Thr Gln Gly 
    50                  55                  60                  


Asp Arg Gly Val Gly Ser Ser Ala Val Ile Leu Asp Asp Asn Phe Val 
65                  70                  75                  80  


Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val Asn Tyr Ser Ser 
                85                  90                  95      


Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser Arg Tyr Phe Thr 
            100                 105                 110         


Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe Gln Pro Asn Asn 
        115                 120                 125             


Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ala Gly Asn Val Asp 
    130                 135                 140                 


His Val Gly Leu Gly Thr 
145                 150 


<210>  13
<211>  154
<212>  PRT
<213>  Porcine circovirus - CN

<400>  13

Thr Arg Leu Ser Arg Thr Ile Gly Tyr Thr Val Lys Lys Thr Thr Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Asn Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Leu Pro Pro Gly Gly Gly Ser Asn Pro Leu Thr Val Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Asn Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Arg Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Thr 
    130                 135                 140                 


Gly Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  14
<211>  154
<212>  PRT
<213>  Porcine circovirus - CN

<400>  14

Thr Arg Leu Ser Arg Thr Ile Gly Tyr Thr Val Lys Lys Thr Thr Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Asn Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Leu Pro Pro Gly Gly Gly Ser Asn Pro Leu Thr Met Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Asn Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Arg Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Thr 
    130                 135                 140                 


Gly Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  15
<211>  154
<212>  PRT
<213>  Porcine circovirus - CN

<400>  15

Ala Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Arg Thr Thr Val 
1               5                   10                  15      


Thr Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Lys Leu Asp Asp 
            20                  25                  30          


Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Pro Lys Ala Asn Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Phe Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Met Arg Ile Gln Thr Ser 
    130                 135                 140                 


Arg Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  16
<211>  154
<212>  PRT
<213>  Porcine circovirus - BR

<400>  16

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Arg Thr Thr Val 
1               5                   10                  15      


Thr Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Lys Leu Asp Asp 
            20                  25                  30          


Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Pro Lys Ala Asn Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Lys Asn Gln Leu Trp Leu Arg Leu Gln Thr Ser 
    130                 135                 140                 


Arg Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  17
<211>  154
<212>  PRT
<213>  Porcine circovirus - US

<400>  17

Ala Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Ala Thr Thr Val 
1               5                   10                  15      


Ser Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Leu Asp Asp 
            20                  25                  30          


Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Ser Ala Ile Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Ile Lys Ala Thr Ala Gln Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Met Arg Leu Gln Thr Ser 
    130                 135                 140                 


Arg Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  18
<211>  154
<212>  PRT
<213>  Porcine circovirus - CA

<400>  18

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Ala Thr Thr Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asp Asp 
            20                  25                  30          


Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Met Arg Leu Gln Thr Ser 
    130                 135                 140                 


Arg Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  19
<211>  154
<212>  PRT
<213>  Porcine circovirus - TW

<400>  19

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Ala Thr Thr Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Val Pro Pro Gly Gly Gly Thr Asn Lys Ile Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Pro Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ser 
    130                 135                 140                 


Ala Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


<210>  20
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Porcine circovirus - consensus sequence

<400>  20

Thr Arg Leu Ser Arg Thr Phe Gly Tyr Thr Val Lys Arg Thr Pro Val 
1               5                   10                  15      


Arg Thr Pro Ser Trp Ala Val Asp Met Met Arg Phe Asn Ile Asn Asp 
            20                  25                  30          


Phe Leu Pro Pro Gly Gly Gly Ser Asn His Arg Ser Ile Pro Phe Glu 
        35                  40                  45              


Tyr Tyr Arg Ile Arg Lys Val Lys Val Glu Phe Trp Pro Cys Ser Pro 
    50                  55                  60                  


Ile Thr Gln Gly Asp Arg Gly Val Gly Ser Thr Ala Val Ile Leu Asp 
65                  70                  75                  80  


Asp Asn Phe Val Thr Lys Ala Thr Ala Leu Thr Tyr Asp Pro Tyr Val 
                85                  90                  95      


Asn Tyr Ser Ser Arg His Thr Ile Thr Gln Pro Phe Ser Tyr His Ser 
            100                 105                 110         


Arg Tyr Phe Thr Pro Lys Pro Val Leu Asp Ser Thr Ile Asp Tyr Phe 
        115                 120                 125             


Gln Pro Asn Asn Lys Arg Asn Gln Leu Trp Leu Arg Leu Gln Thr Ser 
    130                 135                 140                 


Ala Asn Val Asp His Val Gly Leu Gly Thr 
145                 150                 


