                         SEQUENCE LISTING

<110>  GNOSIS S.p.A.
 
<120>  Biotechnological production of chondroitin

<130>  G71589

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1566
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(1566)

<400>  1
atgcttctaa taatgtctgg ttcctatgtt caacaagaat taggggccga atttggttct       60

attcctccaa gctttcttcc tttagctaat aaacgattat ttaagcatca agtatcttta      120

gggcatgatg gtcatgcaat atatctggtt ttaccggaag attttgtgtt tgacaaacat      180

gattatgaat ggttgcttcg taataaagta acaatgatcc ctgtcgatag taacttgaca      240

ttagggcaag cgatagttac cgcatggaat ttaataggag ataaagatga caaaggctta      300

caattattgt ttggcgatac actctttaaa aaaattcctg caggggagga attagtagca      360

aaaagtcatc ctgatgaaaa ttatcaatgg gccatttttt acgaaacaga gttaagagcc      420

gtcagtagag aagataataa aaatgtaatt tgtgggtatt tttcttttag aaaaccgaat      480

ttttttatta gggaattagt tacttcaaaa tttgatttta cggcggcact taaaaagtat      540

cacgacagct atagtttagc ctctatatac gtgtctgatt ggcttgattt tggacatatt      600

aatacatact ataagtcaaa agtacaatac acaacccagc gtgcatttaa tgaattatgc      660

attacaacaa aatccgttat caaatcaagt tcaaatgaaa gtaaaattga agctgaatca      720

aaatggtttg aaactattcc cggagaatta aagatctata ctccaatgtt attggaaccg      780

tttgatcata tcagaaagag ttataagctt gaatatttat ataatacgac gttaaatgaa      840

ttatttgttt tttctcgcct accaaataat attttaacaa atatattaat aagttgttta      900

gacttcatcg atctgtgcaa agaatatcat tcaattgata ctgacaaaaa tatactgcaa      960

gatttatttt atgaaaaaac gattgagcgg gttagcaagt acataacaga tttaaatatt     1020

gatccaaatg caaaatggaa ttttaataat aatataagcg tttcaattaa tgatattctt     1080

tatgatacta ataaatttat cccaagtgaa ctgcaatata aaactattat gcatggcgat     1140

ttatgcttta gtaatataat ttttaacttt agaactggta gaatacaagt ttttgatccc     1200

agaggattga accactctgg agaaataagt atttatggtg attttcgtta tgatatagct     1260

aaattatcac attcaatact agggctctat gattggataa ttgcaggata ttatataata     1320

aataaaaaaa ataaaactca tagtattgaa ttcaaaatta atattgataa taaattgttt     1380

gaaattcaat caacatttgt ttctataata aaagagaaat attcaatctc cgaaaaatca     1440

ttgtatgcga tgcaaataca tttattttta tcaatgcttc cccttcattc cgatgacaaa     1500

aaaaggcaag atgcactatt tgctaatgca tttagattat atgaaatttt taaggaggct     1560

gcagta                                                                1566


<210>  2
<211>  522
<212>  PRT
<213>  Escherichia coli


<220>
<221>  protein
<222>  (1)..(522)

<400>  2

Met Leu Leu Ile Met Ser Gly Ser Tyr Val Gln Gln Glu Leu Gly Ala 
1               5                   10                  15      


Glu Phe Gly Ser Ile Pro Pro Ser Phe Leu Pro Leu Ala Asn Lys Arg 
            20                  25                  30          


Leu Phe Lys His Gln Val Ser Leu Gly His Asp Gly His Ala Ile Tyr 
        35                  40                  45              


Leu Val Leu Pro Glu Asp Phe Val Phe Asp Lys His Asp Tyr Glu Trp 
    50                  55                  60                  


Leu Leu Arg Asn Lys Val Thr Met Ile Pro Val Asp Ser Asn Leu Thr 
65                  70                  75                  80  


Leu Gly Gln Ala Ile Val Thr Ala Trp Asn Leu Ile Gly Asp Lys Asp 
                85                  90                  95      


Asp Lys Gly Leu Gln Leu Leu Phe Gly Asp Thr Leu Phe Lys Lys Ile 
            100                 105                 110         


Pro Ala Gly Glu Glu Leu Val Ala Lys Ser His Pro Asp Glu Asn Tyr 
        115                 120                 125             


Gln Trp Ala Ile Phe Tyr Glu Thr Glu Leu Arg Ala Val Ser Arg Glu 
    130                 135                 140                 


Asp Asn Lys Asn Val Ile Cys Gly Tyr Phe Ser Phe Arg Lys Pro Asn 
145                 150                 155                 160 


Phe Phe Ile Arg Glu Leu Val Thr Ser Lys Phe Asp Phe Thr Ala Ala 
                165                 170                 175     


Leu Lys Lys Tyr His Asp Ser Tyr Ser Leu Ala Ser Ile Tyr Val Ser 
            180                 185                 190         


Asp Trp Leu Asp Phe Gly His Ile Asn Thr Tyr Tyr Lys Ser Lys Val 
        195                 200                 205             


Gln Tyr Thr Thr Gln Arg Ala Phe Asn Glu Leu Cys Ile Thr Thr Lys 
    210                 215                 220                 


Ser Val Ile Lys Ser Ser Ser Asn Glu Ser Lys Ile Glu Ala Glu Ser 
225                 230                 235                 240 


Lys Trp Phe Glu Thr Ile Pro Gly Glu Leu Lys Ile Tyr Thr Pro Met 
                245                 250                 255     


Leu Leu Glu Pro Phe Asp His Ile Arg Lys Ser Tyr Lys Leu Glu Tyr 
            260                 265                 270         


Leu Tyr Asn Thr Thr Leu Asn Glu Leu Phe Val Phe Ser Arg Leu Pro 
        275                 280                 285             


Asn Asn Ile Leu Thr Asn Ile Leu Ile Ser Cys Leu Asp Phe Ile Asp 
    290                 295                 300                 


Leu Cys Lys Glu Tyr His Ser Ile Asp Thr Asp Lys Asn Ile Leu Gln 
305                 310                 315                 320 


Asp Leu Phe Tyr Glu Lys Thr Ile Glu Arg Val Ser Lys Tyr Ile Thr 
                325                 330                 335     


Asp Leu Asn Ile Asp Pro Asn Ala Lys Trp Asn Phe Asn Asn Asn Ile 
            340                 345                 350         


Ser Val Ser Ile Asn Asp Ile Leu Tyr Asp Thr Asn Lys Phe Ile Pro 
        355                 360                 365             


Ser Glu Leu Gln Tyr Lys Thr Ile Met His Gly Asp Leu Cys Phe Ser 
    370                 375                 380                 


Asn Ile Ile Phe Asn Phe Arg Thr Gly Arg Ile Gln Val Phe Asp Pro 
385                 390                 395                 400 


Arg Gly Leu Asn His Ser Gly Glu Ile Ser Ile Tyr Gly Asp Phe Arg 
                405                 410                 415     


Tyr Asp Ile Ala Lys Leu Ser His Ser Ile Leu Gly Leu Tyr Asp Trp 
            420                 425                 430         


Ile Ile Ala Gly Tyr Tyr Ile Ile Asn Lys Lys Asn Lys Thr His Ser 
        435                 440                 445             


Ile Glu Phe Lys Ile Asn Ile Asp Asn Lys Leu Phe Glu Ile Gln Ser 
    450                 455                 460                 


Thr Phe Val Ser Ile Ile Lys Glu Lys Tyr Ser Ile Ser Glu Lys Ser 
465                 470                 475                 480 


Leu Tyr Ala Met Gln Ile His Leu Phe Leu Ser Met Leu Pro Leu His 
                485                 490                 495     


Ser Asp Asp Lys Lys Arg Gln Asp Ala Leu Phe Ala Asn Ala Phe Arg 
            500                 505                 510         


Leu Tyr Glu Ile Phe Lys Glu Ala Ala Val 
        515                 520         


<210>  3
<211>  60
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL151


<220>
<221>  oligonucleotide
<222>  (1)..(60)

<400>  3
atgcttctaa taatgtctgg ttcctatgtt caacaagaat gtgtaggctg gagctgcttc       60


<210>  4
<211>  62
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL152


<220>
<221>  oligonucleotide
<222>  (1)..(62)

<400>  4
tcatactgca gcctccttaa aaatttcata taatctaaat gcacatatga atatcctcct       60

ta                                                                      62


<210>  5
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL149


<220>
<221>  oligonucleotide
<222>  (1)..(22)

<400>  5
ccactcataa atcctcatag ag                                                22


<210>  6
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL150


<220>
<221>  oligonucleotide
<222>  (1)..(22)

<400>  6
ccaacttact tctgacaacg at                                                22


<210>  7
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL153


<220>
<221>  oligonucleotide
<222>  (1)..(22)

<400>  7
aatccgacgg ggactgtaga tt                                                22


<210>  8
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL142


<220>
<221>  oligonucleotide
<222>  (1)..(20)

<400>  8
aactgttcgc caggctcaag                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL143


<220>
<221>  oligonucleotide
<222>  (1)..(20)

<400>  9
gcgttttccc ttgtccagat                                                   20


<210>  10
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL154


<220>
<221>  oligonucleotide
<222>  (1)..(23)

<400>  10
gctaatgtat atgattgcca ggt                                               23


<210>  11
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL169


<220>
<221>  oligonucleotide
<222>  (1)..(27)

<400>  11
tgaggtgatt gttggtaaac cttggtg                                           27


<210>  12
<211>  25
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL166


<220>
<221>  oligonucleotide
<222>  (1)..(25)

<400>  12
tactgtttct gcttgccccc gagtt                                             25


<210>  13
<211>  167
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(167)
<223>  rearranged

<400>  13
atgcttctaa taatgtctgg ttcctatgtt caacaagaat gtgtaggctg gagctgcttc       60

gaagttccta tactttctag agaataggaa cttcggaata ggaactaagg aggatattca      120

tatgtgcatt tagattatat gaaattttta aggaggctgc agtatga                    167


<210>  14
<211>  1559
<212>  DNA
<213>  Escherichia coli


<220>
<221>  gene
<222>  (1)..(1559)
<223>  rearranged

<400>  14
atgcttctaa taatgtctgg ttcctatgtt caacaagaat gtgtaggctg gagctgcttc       60

gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa gatcccccac      120

gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca      180

gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg      240

aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag      300

actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta      360

aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc      420

gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag      480

atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg      540

cacaacagac aatcggctgc tctaatgccg ccgtgttccg gctgtcagcg caggggcgcc      600

cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag      660

cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca      720

ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat      780

ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata      840

cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac      900

gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc      960

tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg     1020

tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg     1080

gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta     1140

cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg     1200

gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct     1260

gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga     1320

tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc     1380

cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccagctt     1440

caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaactaa     1500

ggaggatatt catatgtgca tttagattat atgaaatttt taaggaggct gcagtatga      1559


<210>  15
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL172


<220>
<221>  oligonucleotide
<222>  (1)..(36)

<400>  15
acaacatgtt actaataatg tctggttcct atgttc                                 36


<210>  16
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  OL173


<220>
<221>  oligonucleotide
<222>  (1)..(31)

<400>  16
actggatcct tatcatactg cagcctcctt a                                      31


