                         SEQUENCE LISTING

<110>  Draths Corporation
       Frost, John W.
 
<120>  PHLOROGLUCINOL SYNTHASES AND METHODS OF MAKING AND USING THE SAME

<130>  127651-012501/PCT

<150>  US 61/398,897
<151>  2010-07-02

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1050
<212>  DNA
<213>  Pseudomonas fluorescens Pf-5


<220>
<221>  CDS
<222>  (1)..(1050)
<223>  Phloroglucinol synthase ORF

<400>  1
atg tct aca ctt tgc ctt cca cac gtc atg ttt ccg caa cac aag atc       48
Met Ser Thr Leu Cys Leu Pro His Val Met Phe Pro Gln His Lys Ile         
1               5                   10                  15              

acc cag caa cag atg gtc gat cac ctg gaa aac ctg cac gcc gac cat       96
Thr Gln Gln Gln Met Val Asp His Leu Glu Asn Leu His Ala Asp His         
            20                  25                  30                  

cca cgc atg gcc ctg gcc aag cgc atg atc gcc aac acc gaa gtc aac      144
Pro Arg Met Ala Leu Ala Lys Arg Met Ile Ala Asn Thr Glu Val Asn         
        35                  40                  45                      

gag cgc cac ctg gtg ttg ccg atc gac gaa ctg gca gtg cac acc ggt      192
Glu Arg His Leu Val Leu Pro Ile Asp Glu Leu Ala Val His Thr Gly         
    50                  55                  60                          

ttc acc cac cgc agc atc gtc tac gag cgt gaa gcc cgg cag atg tcg      240
Phe Thr His Arg Ser Ile Val Tyr Glu Arg Glu Ala Arg Gln Met Ser         
65                  70                  75                  80          

tcg gcc gcg gcg cgc cag gcc atc gag aat gcc ggg ctg cag atc agc      288
Ser Ala Ala Ala Arg Gln Ala Ile Glu Asn Ala Gly Leu Gln Ile Ser         
                85                  90                  95              

gac att cgc atg gtg atc gtc act tcc tgc acc ggc ttc atg atg ccg      336
Asp Ile Arg Met Val Ile Val Thr Ser Cys Thr Gly Phe Met Met Pro         
            100                 105                 110                 

tcg ctg acc gcg cac ctg atc aac gac ctg gcc ctg cca acc tcc acc      384
Ser Leu Thr Ala His Leu Ile Asn Asp Leu Ala Leu Pro Thr Ser Thr         
        115                 120                 125                     

gtg cag ttg ccg atc gcc cag ctg ggc tgc gtg gcc ggt gcc gcg gcc      432
Val Gln Leu Pro Ile Ala Gln Leu Gly Cys Val Ala Gly Ala Ala Ala         
    130                 135                 140                         

atc aac cgc gcc aac gac ttc gcc cgg ctc gat gcc cgc aac cac gta      480
Ile Asn Arg Ala Asn Asp Phe Ala Arg Leu Asp Ala Arg Asn His Val         
145                 150                 155                 160         

ctg atc gtg tcc ctg gaa ttc tcc tcg ctg tgc tac cag ccg gac gac      528
Leu Ile Val Ser Leu Glu Phe Ser Ser Leu Cys Tyr Gln Pro Asp Asp         
                165                 170                 175             

acc aag ctg cac gcc ttc atc tcc gcg gcg ctg ttc ggc gat gcg gta      576
Thr Lys Leu His Ala Phe Ile Ser Ala Ala Leu Phe Gly Asp Ala Val         
            180                 185                 190                 

tcc gcc tgc gtg ctg cgc gcc gat gac cag gcc ggc ggc ttc aag atc      624
Ser Ala Cys Val Leu Arg Ala Asp Asp Gln Ala Gly Gly Phe Lys Ile         
        195                 200                 205                     

aag aag acc gag tcg tac ttc ctg ccc aag agc gag cac tac atc aag      672
Lys Lys Thr Glu Ser Tyr Phe Leu Pro Lys Ser Glu His Tyr Ile Lys         
    210                 215                 220                         

tac gac gtg aag gac acc ggc ttt cac ttc acc ctc gac aag gcg gtg      720
Tyr Asp Val Lys Asp Thr Gly Phe His Phe Thr Leu Asp Lys Ala Val         
225                 230                 235                 240         

atg aac tcc atc aag gac gtg gca ccg gtc atg gag cgg ctc aac tac      768
Met Asn Ser Ile Lys Asp Val Ala Pro Val Met Glu Arg Leu Asn Tyr         
                245                 250                 255             

gag agc ttc gaa cag aac tgt gcg cac aac gac ttc ttc atc ttc cac      816
Glu Ser Phe Glu Gln Asn Cys Ala His Asn Asp Phe Phe Ile Phe His         
            260                 265                 270                 

acc ggt ggt cgc aag atc ctc gac gag ctg gtg atg cac ctg gac ctg      864
Thr Gly Gly Arg Lys Ile Leu Asp Glu Leu Val Met His Leu Asp Leu         
        275                 280                 285                     

gca tcc aac cgg gtc tcg caa tcg cgc agc agc ctg tcg gaa gcc ggc      912
Ala Ser Asn Arg Val Ser Gln Ser Arg Ser Ser Leu Ser Glu Ala Gly         
    290                 295                 300                         

aac att gcc agc gtg gtg gtg ttc gac gta ctc aag cgg cag ttc gat      960
Asn Ile Ala Ser Val Val Val Phe Asp Val Leu Lys Arg Gln Phe Asp         
305                 310                 315                 320         

tcc aac ctc aat cgc ggc gac atc ggc ctg ctg gca gcc ttc ggc ccg     1008
Ser Asn Leu Asn Arg Gly Asp Ile Gly Leu Leu Ala Ala Phe Gly Pro         
                325                 330                 335             

ggg ttc acc gcg gaa atg gcg gtg ggc gag tgg acc gcc tga             1050
Gly Phe Thr Ala Glu Met Ala Val Gly Glu Trp Thr Ala                     
            340                 345                                     


<210>  2
<211>  349
<212>  PRT
<213>  Pseudomonas fluorescens Pf-5

<400>  2

Met Ser Thr Leu Cys Leu Pro His Val Met Phe Pro Gln His Lys Ile 
1               5                   10                  15      


Thr Gln Gln Gln Met Val Asp His Leu Glu Asn Leu His Ala Asp His 
            20                  25                  30          


Pro Arg Met Ala Leu Ala Lys Arg Met Ile Ala Asn Thr Glu Val Asn 
        35                  40                  45              


Glu Arg His Leu Val Leu Pro Ile Asp Glu Leu Ala Val His Thr Gly 
    50                  55                  60                  


Phe Thr His Arg Ser Ile Val Tyr Glu Arg Glu Ala Arg Gln Met Ser 
65                  70                  75                  80  


Ser Ala Ala Ala Arg Gln Ala Ile Glu Asn Ala Gly Leu Gln Ile Ser 
                85                  90                  95      


Asp Ile Arg Met Val Ile Val Thr Ser Cys Thr Gly Phe Met Met Pro 
            100                 105                 110         


Ser Leu Thr Ala His Leu Ile Asn Asp Leu Ala Leu Pro Thr Ser Thr 
        115                 120                 125             


Val Gln Leu Pro Ile Ala Gln Leu Gly Cys Val Ala Gly Ala Ala Ala 
    130                 135                 140                 


Ile Asn Arg Ala Asn Asp Phe Ala Arg Leu Asp Ala Arg Asn His Val 
145                 150                 155                 160 


Leu Ile Val Ser Leu Glu Phe Ser Ser Leu Cys Tyr Gln Pro Asp Asp 
                165                 170                 175     


Thr Lys Leu His Ala Phe Ile Ser Ala Ala Leu Phe Gly Asp Ala Val 
            180                 185                 190         


Ser Ala Cys Val Leu Arg Ala Asp Asp Gln Ala Gly Gly Phe Lys Ile 
        195                 200                 205             


Lys Lys Thr Glu Ser Tyr Phe Leu Pro Lys Ser Glu His Tyr Ile Lys 
    210                 215                 220                 


Tyr Asp Val Lys Asp Thr Gly Phe His Phe Thr Leu Asp Lys Ala Val 
225                 230                 235                 240 


Met Asn Ser Ile Lys Asp Val Ala Pro Val Met Glu Arg Leu Asn Tyr 
                245                 250                 255     


Glu Ser Phe Glu Gln Asn Cys Ala His Asn Asp Phe Phe Ile Phe His 
            260                 265                 270         


Thr Gly Gly Arg Lys Ile Leu Asp Glu Leu Val Met His Leu Asp Leu 
        275                 280                 285             


Ala Ser Asn Arg Val Ser Gln Ser Arg Ser Ser Leu Ser Glu Ala Gly 
    290                 295                 300                 


Asn Ile Ala Ser Val Val Val Phe Asp Val Leu Lys Arg Gln Phe Asp 
305                 310                 315                 320 


Ser Asn Leu Asn Arg Gly Asp Ile Gly Leu Leu Ala Ala Phe Gly Pro 
                325                 330                 335     


Gly Phe Thr Ala Glu Met Ala Val Gly Glu Trp Thr Ala 
            340                 345                 


<210>  3
<211>  1047
<212>  DNA
<213>  Vibrio cholarae

<400>  3
atgatgccta cgctgtgtct gccggaatct ctgtacccaa ttaacgtggt tactaacgaa       60

gacattgtta acttcatcaa cctgcaccac ccggatatcc gccacaaaga tcgtgctttc      120

gaaatgatcc ataacactac gattgaagaa cgtcacacga tcatcccttt tgatgagatc      180

atgaaactgg aaaacttcgg tcagcgtggt gaactgtacg aaaaacactc taagatgctg      240

gccattaaag cggccgttaa agcgctgtct aacgcggaac tgcaaccgga agaaatcagc      300

atggtaattg ttactagctg caccggcttc atgatgccgt ctctgaccgc ctacctgatt      360

aaccagctga atctgcgtga caataccatt cagctgccta tttcccagat gggctgtgtt      420

ggcggtgcgt tcgcaattaa ccgtgcctac gaacactgtc tgcaaagcaa gaaaaacaat      480

gtactgatcg tttccgtaga aacctcctct ctgtgctttc accgtcaggc ggatcgtctg      540

caagatttca tttctgacgc gctgttcggt gatggtgtcg cgtccgtggt aatgcgtggt      600

gatgacaacc tgtctggctt taaaatcctg gcgaaacatg gctttatgat caaagacact      660

gaatcttaca tcaaatacgg tatcactgac cagggtttcc acttctccct ggacaaagaa      720

gttatgtact ccgttgaact ggctgccccg gaaatggaaa aattcatcgt ccaggaactg      780

ggcggtctgg atcagatcga cttttacatt tcccacactg gtggtcgtcg catcctggac      840

gaggttgaac gctgcctgca gctgccgcca agccacctgc accatagccg cgaatgcctg      900

cgtctgaccg gtaacacctc ctctgttgcc gtgctggacg tactgtctcg tcacttctcc      960

gagcgtaaac agggtgaccg tggtgtcatc tctgcttttg gcccgggttt caccactgaa     1020

ctggctatcg gcgtttggta atgactc                                         1047



<210>  4
<211>  1083
<212>  DNA
<213>  Yersinia mollaretii

<400>  4

atgatgggct actccctgaa taacatttcc gttcctacta tttgtaaacc ggcgatcatc       60

ctgccagaat actctgttac gcaggctgaa attatcgagt ttatcaaagt acacttcccg      120

aacgccaacg cactgccaaa ggcgattaaa atgattagca acaaccaagt gctgaaacgc      180

aacttcatcc gtccgctgaa agaaatcatc tccgatctgg gcctggaaca gcgtaatagc      240

ctgtacctga agttcgcccg tcagctggct aaaaaggcgg cagtccaggc tattaagaac      300

gcggaactgg aaattaacga aattgatatg gttatcgcaa cttcttgtac gggctttctg      360

atgccgtccc tgacgaccat gctgattaac gacctgggcc tgtctgcaaa aacccgccaa      420

gtgccgatct cccaactggg ttgctccgct ggtacggctg cggtgaacct ggcagtggac      480

cactgtaacg cgcattccgg taataacgta ctgatcgtaa acgttgaact gtgctctctg      540

ctgttccacg cagaggacgc tctgctgtct tctctgatca gcgcttccct gtttggtgac      600

gcggcgaccg cgtctgtagt gcgtggcaaa ggtggcaaag gtttcgcgat taccgcaacc      660

cattctttca tgctgcgtaa caccgaggat tacatctatt acgacatcaa agacgctggt      720

ttctacttcc gtctgaataa aaaggttatg tcttccatca aactggtgct gccggaagtt      780

actcagttcc tgaaatccac ctaccagaaa gaagcgaacg agatggattt tcacgtgttc      840

cataccggcg gcaaacgcat cctggatgaa ctggctaacg gttttagcct ggaagaaaaa      900

tccatcatca attctcgcga aagcctgagc gaaaacggta acctgtcctc tgcagcggtg      960

tttgatgtgc tgaaccgttt cttcgaaaac ggtcgtccgc tgccaggcca acagggtttc     1020

atgctggcat tcggtccggg tttcagcacc gaaatttctg cgggttattg ggtttaatga     1080

ctc                                                                   1083


<210>  5
<211>  345
<212>  PRT
<213>  Vibrio cholerae

<400>  6

Met Pro Thr Leu Cys Leu Pro Glu Ser Leu Tyr Pro Ile Asn Val Val 
1               5                   10                  15      


Thr Asn Glu Asp Ile Val Asn Phe Ile Asn Leu His His Pro Asp Ile 
            20                  25                  30          


Arg His Lys Asp Arg Ala Phe Glu Met Ile His Asn Thr Thr Ile Glu 
        35                  40                  45              


Glu Arg His Thr Ile Ile Pro Phe Asp Glu Ile Met Lys Leu Glu Asn 
    50                  55                  60                  


Phe Gly Gln Arg Gly Glu Leu Tyr Glu Lys His Ser Lys Met Leu Ala 
65                  70                  75                  80  


Ile Lys Ala Ala Val Lys Ala Leu Ser Asn Ala Glu Leu Gln Pro Glu 
                85                  90                  95      


Glu Ile Ser Met Val Ile Val Thr Ser Cys Thr Gly Phe Met Met Pro 
            100                 105                 110         


Ser Leu Thr Ala Tyr Leu Ile Asn Gln Leu Asn Leu Arg Asp Asn Thr 
        115                 120                 125             


Ile Gln Leu Pro Ile Ser Gln Met Gly Cys Val Gly Gly Ala Phe Ala 
    130                 135                 140                 


Ile Asn Arg Ala Tyr Glu His Cys Leu Gln Ser Lys Lys Asn Asn Val 
145                 150                 155                 160 


Leu Ile Val Ser Val Glu Thr Ser Ser Leu Cys Phe His Arg Gln Ala 
                165                 170                 175     


Asp Arg Leu Gln Asp Phe Ile Ser Asp Ala Leu Phe Gly Asp Gly Val 
            180                 185                 190         


Ala Ser Val Val Met Arg Gly Asp Asp Asn Leu Ser Gly Phe Lys Ile 
        195                 200                 205             


Leu Ala Lys His Gly Phe Met Ile Lys Asp Thr Glu Ser Tyr Ile Lys 
    210                 215                 220                 


Tyr Gly Ile Thr Asp Gln Gly Phe His Phe Ser Leu Asp Lys Glu Val 
225                 230                 235                 240 


Met Tyr Ser Val Glu Leu Ala Ala Pro Glu Met Glu Lys Phe Ile Val 
                245                 250                 255     


Gln Glu Leu Gly Gly Leu Asp Gln Ile Asp Phe Tyr Ile Ser His Thr 
            260                 265                 270         


Gly Gly Arg Arg Ile Leu Asp Glu Val Glu Arg Cys Leu Gln Leu Pro 
        275                 280                 285             


Pro Ser His Leu His His Ser Arg Glu Cys Leu Arg Leu Thr Gly Asn 
    290                 295                 300                 


Thr Ser Ser Val Ala Val Leu Asp Val Leu Ser Arg His Phe Ser Glu 
305                 310                 315                 320 


Arg Lys Gln Gly Asp Arg Gly Val Ile Ser Ala Phe Gly Pro Gly Phe 
                325                 330                 335     


Thr Thr Glu Leu Ala Ile Gly Val Trp 
            340                 345 


<210>  6
<211>  356
<212>  PRT
<213>  Yersinia mollaretii

<400>  6

Gly Tyr Ser Leu Asn Asn Ile Ser Val Pro Thr Ile Cys Lys Pro Ala 
1               5                   10                  15      


Ile Ile Leu Pro Glu Tyr Ser Val Thr Gln Ala Glu Ile Ile Glu Phe 
            20                  25                  30          


Ile Lys Val His Phe Pro Asn Ala Asn Ala Leu Pro Lys Ala Ile Lys 
        35                  40                  45              


Met Ile Ser Asn Asn Gln Val Leu Lys Arg Asn Phe Ile Arg Pro Leu 
    50                  55                  60                  


Lys Glu Ile Ile Ser Asp Leu Gly Leu Glu Gln Arg Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Lys Phe Ala Arg Gln Leu Ala Lys Lys Ala Ala Val Gln Ala Ile 
                85                  90                  95      


Lys Asn Ala Glu Leu Glu Ile Asn Glu Ile Asp Met Val Ile Ala Thr 
            100                 105                 110         


Ser Cys Thr Gly Phe Leu Met Pro Ser Leu Thr Thr Met Leu Ile Asn 
        115                 120                 125             


Asp Leu Gly Leu Ser Ala Lys Thr Arg Gln Val Pro Ile Ser Gln Leu 
    130                 135                 140                 


Gly Cys Ser Ala Gly Thr Ala Ala Val Asn Leu Ala Val Asp His Cys 
145                 150                 155                 160 


Asn Ala His Ser Gly Asn Asn Val Leu Ile Val Asn Val Glu Leu Cys 
                165                 170                 175     


Ser Leu Leu Phe His Ala Glu Asp Ala Leu Leu Ser Ser Leu Ile Ser 
            180                 185                 190         


Ala Ser Leu Phe Gly Asp Ala Ala Thr Ala Ser Val Val Arg Gly Lys 
        195                 200                 205             


Gly Gly Lys Gly Phe Ala Ile Thr Ala Thr His Ser Phe Met Leu Arg 
    210                 215                 220                 


Asn Thr Glu Asp Tyr Ile Tyr Tyr Asp Ile Lys Asp Ala Gly Phe Tyr 
225                 230                 235                 240 


Phe Arg Leu Asn Lys Lys Val Met Ser Ser Ile Lys Leu Val Leu Pro 
                245                 250                 255     


Glu Val Thr Gln Phe Leu Lys Ser Thr Tyr Gln Lys Glu Ala Asn Glu 
            260                 265                 270         


Met Asp Phe His Val Phe His Thr Gly Gly Lys Arg Ile Leu Asp Glu 
        275                 280                 285             


Leu Ala Asn Gly Phe Ser Leu Glu Glu Lys Ser Ile Ile Asn Ser Arg 
    290                 295                 300                 


Glu Ser Leu Ser Glu Asn Gly Asn Leu Ser Ser Ala Ala Val Phe Asp 
305                 310                 315                 320 


Val Leu Asn Arg Phe Phe Glu Asn Gly Arg Pro Leu Pro Gly Gln Gln 
                325                 330                 335     


Gly Phe Met Leu Ala Phe Gly Pro Gly Phe Ser Thr Glu Ile Ser Ala 
            340                 345                 350         


Gly Tyr Trp Val 
        355     

