                         SEQUENCE LISTING

<110>  Codexis, Inc.
       ALVIZO, Oscar
 
<120>  HIGHLY STABLE BETA-CLASS CARBONIC ANHYDRASES USEFUL IN CARBON 
       CAPTURE SYSTEMS

<130>  CX4-083US1

<150>  US 61/360,040
<151>  2010-06-30

<150>  US 61/445,996
<151>  2011-02-23

<160>  1296  

<170>  PatentIn version 3.5

<210>  1
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized carbonic anhydrase gene from Desulfovibrio 
       vulgaris

<400>  1
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  2
<211>  223
<212>  PRT
<213>  Desulfovibrio vulgaris str. "Miyazaki F"

<400>  2

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  3
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  3
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  4
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  4

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  5
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  5
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  6
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  6

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  7
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  7
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  8
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  8

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  9
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  9
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaaagg      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  10
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  10

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  11
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  11
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcaa cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  12
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  12

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  13
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  13
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  14
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  14

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  15
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  15
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  16
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  16

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  17
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  17
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  18
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  18

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  19
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  19
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  20
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  20

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  21
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  21
atgggtagca tgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctaaa cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  22
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  22

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Lys Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  23
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  23
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  24
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  24

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  25
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  25
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  26
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  26

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  27
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  27
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  28
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  28

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  29
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  29
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta cttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  30
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  30

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  31
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  31
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  32
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  32

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  33
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  33
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  34
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  34

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  35
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  35
atgggtagca cgggaccggg aattggacca gacgaagctt tacagagtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  36
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  36

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Ser 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  37
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  37
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  38
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  38

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  39
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  39
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  40
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  40

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  41
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  41
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgtcgct aa                                                          672


<210>  42
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  42

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  43
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  43
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtattgaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  44
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  44

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  45
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  45
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  46
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  46

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  47
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  47
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccacagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  48
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  48

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  49
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  49
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagt tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  50
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  50

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  51
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  51
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  52
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  52

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  53
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  53
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  54
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  54

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  55
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  55
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aacagaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  56
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  56

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  57
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  57
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  58
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  58

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  59
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  59
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagcaga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  60
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  60

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  61
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  61
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  62
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  62

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  63
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  63
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  64
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  64

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  65
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  65
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcgtaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  66
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  66

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  67
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  67
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  68
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  68

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  69
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  69
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaag gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  70
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  70

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  71
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  71
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgtcaccgct aa                                                          672


<210>  72
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  72

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  73
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  73
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  74
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  74

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ser Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  75
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  75
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  76
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  76

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  77
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  77
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  78
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  78

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  79
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  79
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagttgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  80
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  80

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  81
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  81
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga atacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  82
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  82

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  83
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  83
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  84
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  84

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  85
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  85
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gttgaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  86
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  86

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  87
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  87
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccggg tcacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  88
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  88

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Gly His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  89
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  89
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgtt aa                                                          672


<210>  90
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  90

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  91
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  91
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctgca cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  92
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  92

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Ala Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  93
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  93
atgcgtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  94
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  94

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  95
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  95
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

catgaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  96
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  96

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly His Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  97
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  97
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  98
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  98

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  99
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  99
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagatgt      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  100
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  100

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  101
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  101
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagcctgtca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  102
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  102

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Cys 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  103
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  103
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctttg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  104
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  104

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Leu Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  105
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  105
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgttg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  106
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  106

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  107
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  107
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  108
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  108

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  109
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  109
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgcaggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  110
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  110

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  111
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  111
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcacaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctactaaa      660

gctcaccgct aa                                                          672


<210>  112
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  112

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  113
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  113
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  114
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  114

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  115
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  115
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaacagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  116
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  116

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Thr Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  117
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  117
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  118
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  118

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  119
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  119
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tagcagcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  120
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  120

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  121
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  121
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  122
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  122

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  123
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  123
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaaaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  124
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  124

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Lys Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  125
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  125
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc attgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  126
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  126

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  127
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  127
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgaaacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  128
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  128

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Glu Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  129
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  129
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  130
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  130

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  131
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  131
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

acagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  132
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  132

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Thr Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  133
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  133
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

ttggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  134
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  134

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  135
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  135
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagg caaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  136
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  136

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ala 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  137
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  137
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgtccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  138
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  138

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  139
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  139
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  140
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  140

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  141
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  141
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccaaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  142
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  142

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Lys 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  143
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  143
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag aggtcgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  144
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  144

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Gly Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  145
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  145
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  146
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  146

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  147
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  147
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgcaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  148
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  148

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Ala 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  149
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  149
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  150
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  150

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  151
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  151
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgttgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  152
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  152

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  153
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  153
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaag gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  154
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  154

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  155
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  155
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa agcagaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  156
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  156

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Ala Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  157
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  157
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga ggacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  158
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  158

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  159
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  159
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctgttc atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  160
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  160

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Val His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  161
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  161
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatccgtgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  162
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  162

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Arg Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  163
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  163
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgtt tcacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  164
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  164

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Phe His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  165
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  165
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cggtggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  166
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  166

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gly Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  167
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  167
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc aatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  168
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  168

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  169
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  169
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  170
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  170

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  171
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  171
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atttacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  172
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  172

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Phe Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  173
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  173
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccaat aa                                                          672


<210>  174
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  174

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Gln 
    210                 215                 220             


<210>  175
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  175
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaga acaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  176
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  176

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Glu Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  177
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  177
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  178
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  178

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  179
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  179
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtt      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  180
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  180

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  181
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  181
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgcaa      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  182
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  182

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Gln Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  183
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  183
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatggtagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  184
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  184

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  185
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  185
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagatga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaaa tcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  186
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  186

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Met Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Asn Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  187
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  187
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  188
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  188

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  189
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  189
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  190
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  190

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  191
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  191
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  192
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  192

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  193
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  193
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  194
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  194

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  195
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  195
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  196
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  196

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  197
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  197
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  198
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  198

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  199
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  199
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  200
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  200

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  201
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  201
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  202
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  202

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  203
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  203
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  204
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  204

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  205
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  205
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  206
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  206

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  207
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  207
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  208
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  208

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  209
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  209
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  210
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  210

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  211
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  211
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  212
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  212

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  213
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  213
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  214
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  214

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  215
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  215
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  216
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  216

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  217
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  217
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  218
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  218

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  219
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  219
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  220
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  220

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  221
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  221
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  222
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  222

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  223
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  223
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  224
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  224

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  225
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  225
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  226
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  226

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  227
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  227
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  228
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  228

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  229
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  229
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  230
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  230

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  231
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  231
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  232
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  232

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  233
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  233
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  234
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  234

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  235
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  235
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  236
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  236

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  237
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  237
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  238
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  238

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  239
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  239
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  240
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  240

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  241
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  241
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  242
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  242

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  243
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  243
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  244
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  244

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  245
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  245
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  246
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  246

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  247
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  247
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  248
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  248

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  249
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  249
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  250
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  250

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  251
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  251
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  252
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  252

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  253
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  253
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  254
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  254

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  255
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  255
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  256
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  256

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  257
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  257
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  258
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  258

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  259
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  259
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  260
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  260

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  261
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  261
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  262
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  262

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  263
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  263
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  264
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  264

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  265
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  265
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  266
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  266

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  267
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  267
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  268
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  268

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  269
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  269
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  270
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  270

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  271
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  271
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  272
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  272

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  273
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  273
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  274
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  274

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  275
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  275
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  276
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  276

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  277
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  277
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  278
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  278

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  279
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  279
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  280
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  280

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  281
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  281
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  282
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  282

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  283
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  283
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  284
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  284

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  285
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  285
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  286
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  286

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  287
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  287
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaaa gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  288
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  288

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  289
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  289
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  290
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  290

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  291
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  291
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  292
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  292

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  293
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  293
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  294
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  294

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  295
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  295
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  296
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  296

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  297
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  297
atgaccagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  298
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  298

Met Thr Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  299
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  299
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  300
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  300

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  301
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  301
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  302
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  302

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  303
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  303
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  304
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  304

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  305
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  305
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  306
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  306

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  307
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  307
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  308
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  308

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  309
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  309
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  310
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  310

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  311
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  311
atgaccagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  312
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  312

Met Thr Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  313
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  313
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  314
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  314

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  315
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  315
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  316
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  316

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  317
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  317
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  318
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  318

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  319
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  319
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  320
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  320

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  321
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  321
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag ccgcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  322
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  322

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  323
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  323
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag ccgcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  324
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  324

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  325
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  325
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  326
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  326

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  327
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  327
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  328
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  328

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  329
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  329
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  330
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  330

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  331
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  331
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  332
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  332

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  333
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  333
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  334
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  334

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  335
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  335
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  336
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  336

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  337
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  337
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  338
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  338

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  339
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  339
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  340
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  340

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  341
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  341
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  342
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  342

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  343
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  343
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  344
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  344

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  345
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  345
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  346
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  346

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  347
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  347
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  348
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  348

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  349
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  349
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  350
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  350

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  351
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  351
atgaccagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  352
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  352

Met Thr Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  353
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  353
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  354
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  354

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  355
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  355
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  356
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  356

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  357
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  357
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  358
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  358

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  359
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  359
atgaccagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  360
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  360

Met Thr Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  361
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  361
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctggccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  362
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  362

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  363
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  363
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcctgccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  364
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  364

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Leu Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  365
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  365
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  366
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  366

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  367
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  367
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  368
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  368

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  369
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  369
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gctttccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  370
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  370

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  371
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  371
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  372
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  372

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  373
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  373
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  374
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  374

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  375
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  375
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcctgccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  376
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  376

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Leu Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  377
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  377
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  378
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  378

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  379
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  379
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  380
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  380

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  381
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  381
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  382
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  382

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  383
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  383
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

ctggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  384
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  384

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  385
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  385
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  386
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  386

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  387
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  387
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgtctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  388
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  388

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  389
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  389
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  390
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  390

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  391
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  391
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  392
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  392

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  393
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  393
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gtggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  394
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  394

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  395
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  395
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  396
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  396

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  397
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  397
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  398
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  398

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  399
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  399
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gtggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctggccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  400
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  400

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  401
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  401
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  402
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  402

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  403
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  403
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  404
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  404

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  405
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  405
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gctttccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  406
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  406

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  407
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  407
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gtggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  408
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  408

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  409
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  409
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gtggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  410
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  410

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  411
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  411
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  412
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  412

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  413
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  413
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  414
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  414

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  415
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  415
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  416
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  416

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  417
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  417
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  418
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  418

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  419
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  419
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  420
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  420

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  421
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  421
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  422
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  422

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  423
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  423
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  424
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  424

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  425
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  425
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  426
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  426

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  427
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  427
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gtggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  428
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  428

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  429
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  429
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  430
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  430

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  431
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  431
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  432
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  432

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  433
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  433
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  434
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  434

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  435
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  435
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  436
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  436

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  437
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  437
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  438
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  438

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  439
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  439
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  440
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  440

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  441
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  441
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  442
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  442

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  443
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  443
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  444
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  444

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  445
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  445
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  446
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  446

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  447
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  447
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  448
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  448

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  449
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  449
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  450
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  450

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  451
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  451
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gtggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgga acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  452
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  452

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  453
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  453
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  454
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  454

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  455
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  455
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  456
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  456

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  457
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  457
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  458
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  458

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  459
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  459
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  460
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  460

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  461
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  461
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  462
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  462

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  463
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  463
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  464
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  464

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  465
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  465
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  466
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  466

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  467
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  467
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  468
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  468

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  469
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  469
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  470
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  470

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  471
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  471
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  472
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  472

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  473
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  473
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  474
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  474

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  475
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  475
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  476
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  476

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  477
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  477
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  478
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  478

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  479
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  479
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  480
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  480

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  481
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  481
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  482
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  482

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  483
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  483
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  484
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  484

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  485
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  485
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  486
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  486

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  487
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  487
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  488
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  488

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  489
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  489
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  490
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  490

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  491
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  491
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  492
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  492

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  493
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  493
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  494
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  494

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  495
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  495
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  496
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  496

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  497
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  497
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  498
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  498

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  499
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  499
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  500
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  500

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  501
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  501
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  502
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  502

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  503
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  503
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  504
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  504

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  505
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  505
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tagcggcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgaaacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  506
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  506

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Glu Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  507
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  507
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  508
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  508

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  509
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  509
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  510
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  510

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  511
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  511
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  512
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  512

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  513
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  513
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  514
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  514

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  515
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  515
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  516
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  516

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  517
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  517
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  518
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  518

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  519
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  519
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgaaacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  520
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  520

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Glu Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  521
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  521
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  522
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  522

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  523
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  523
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  524
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  524

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  525
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  525
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  526
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  526

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  527
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  527
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  528
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  528

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  529
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  529
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  530
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  530

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  531
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  531
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  532
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  532

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  533
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  533
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  534
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  534

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  535
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  535
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  536
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  536

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  537
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  537
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  538
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  538

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  539
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  539
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  540
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  540

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  541
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  541
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  542
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  542

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  543
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  543
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgaaacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  544
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  544

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Glu Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  545
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  545
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  546
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  546

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  547
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  547
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  548
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  548

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  549
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  549
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  550
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  550

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  551
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  551
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  552
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  552

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  553
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  553
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  554
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  554

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  555
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  555
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  556
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  556

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  557
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  557
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  558
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  558

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  559
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  559
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  560
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  560

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  561
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  561
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  562
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  562

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  563
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  563
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  564
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  564

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  565
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  565
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgaaacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  566
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  566

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Glu Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  567
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  567
atgggtagca cgggaccggg aattgggcca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc actgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  568
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  568

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  569
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  569
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgctgct aa                                                          672


<210>  570
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  570

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Cys 
    210                 215                 220             


<210>  571
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  571
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtaaaaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  572
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  572

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  573
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  573
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  574
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  574

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  575
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  575
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgctgct aa                                                          672


<210>  576
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  576

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Cys 
    210                 215                 220             


<210>  577
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  577
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  578
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  578

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  579
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  579
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  580
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  580

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  581
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  581
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtat ttggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  582
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  582

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Phe Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  583
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  583
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtaaaaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  584
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  584

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  585
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  585
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  586
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  586

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  587
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  587
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  588
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  588

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  589
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  589
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  590
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  590

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  591
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  591
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  592
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  592

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  593
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  593
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  594
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  594

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  595
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  595
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  596
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  596

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  597
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  597
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  598
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  598

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  599
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  599
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  600
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  600

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  601
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  601
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  602
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  602

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  603
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  603
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  604
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  604

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  605
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  605
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcgtaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  606
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  606

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  607
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  607
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  608
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  608

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  609
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  609
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  610
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  610

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  611
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  611
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtat ttggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  612
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  612

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val Phe Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  613
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  613
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  614
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  614

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  615
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  615
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  616
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  616

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  617
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  617
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  618
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  618

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  619
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  619
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  620
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  620

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  621
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  621
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  622
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  622

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  623
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  623
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtaaaaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  624
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  624

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  625
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  625
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctacaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  626
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  626

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Thr Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  627
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  627
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  628
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  628

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  629
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  629
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  630
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  630

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  631
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  631
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  632
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  632

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  633
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  633
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  634
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  634

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  635
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  635
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  636
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  636

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  637
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  637
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  638
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  638

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  639
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  639
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtat ttggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  640
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  640

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val Phe Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  641
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  641
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  642
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  642

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  643
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  643
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  644
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  644

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  645
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  645
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  646
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  646

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  647
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  647
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

ctggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  648
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  648

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  649
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  649
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtat ttggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  650
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  650

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Phe Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  651
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  651
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtactgaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  652
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  652

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  653
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  653
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  654
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  654

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  655
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  655
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  656
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  656

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  657
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  657
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  658
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  658

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  659
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  659
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  660
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  660

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  661
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  661
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  662
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  662

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  663
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  663
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  664
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  664

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  665
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  665
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  666
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  666

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  667
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  667
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  668
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  668

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  669
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  669
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgccaaa      660

gctcaccgct aa                                                          672


<210>  670
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  670

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  671
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  671
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  672
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  672

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  673
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  673
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  674
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  674

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  675
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  675
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  676
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  676

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  677
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  677
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  678
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  678

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  679
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  679
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  680
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  680

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  681
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  681
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  682
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  682

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  683
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  683
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  684
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  684

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  685
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  685
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  686
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  686

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  687
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  687
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  688
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  688

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  689
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  689
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagcgta gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  690
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  690

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Arg Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  691
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  691
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccagt aa                                                          672


<210>  692
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  692

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Gln 
    210                 215                 220             


<210>  693
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  693
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  694
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  694

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  695
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  695
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  696
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  696

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  697
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  697
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  698
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  698

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  699
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  699
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta tttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  700
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  700

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Phe Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  701
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  701
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  702
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  702

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  703
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  703
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  704
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  704

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  705
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  705
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  706
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  706

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  707
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  707
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  708
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  708

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  709
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  709
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaacc atcgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  710
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  710

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn His Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  711
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  711
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  712
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  712

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  713
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  713
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  714
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  714

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  715
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  715
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  716
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  716

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  717
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  717
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  718
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  718

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  719
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  719
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  720
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  720

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  721
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  721
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctgcag      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  722
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  722

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Gln Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  723
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  723
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  724
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  724

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  725
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  725
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  726
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  726

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  727
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  727
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  728
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  728

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  729
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  729
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  730
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  730

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  731
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  731
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  732
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  732

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  733
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  733
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  734
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  734

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  735
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  735
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  736
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  736

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  737
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  737
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  738
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  738

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  739
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  739
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctaaa ccgcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  740
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  740

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Lys Pro Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  741
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  741
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  742
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  742

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  743
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  743
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  744
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  744

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  745
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  745
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  746
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  746

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  747
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  747
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  748
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  748

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  749
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  749
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  750
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  750

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  751
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  751
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  752
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  752

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  753
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  753
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  754
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  754

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  755
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  755
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  756
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  756

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  757
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  757
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagcgta gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  758
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  758

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Arg Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  759
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  759
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  760
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  760

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  761
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  761
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  762
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  762

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  763
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  763
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagcgta gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  764
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  764

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Arg Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  765
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  765
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  766
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  766

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  767
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  767
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  768
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  768

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  769
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  769
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  770
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  770

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  771
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  771
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc atcgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  772
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  772

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  773
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  773
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  774
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  774

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  775
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  775
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtactgaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  776
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  776

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  777
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  777
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  778
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  778

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  779
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  779
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  780
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  780

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  781
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  781
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  782
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  782

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  783
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  783
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  784
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  784

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  785
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  785
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  786
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  786

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  787
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  787
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  788
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  788

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  789
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  789
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  790
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  790

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  791
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  791
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  792
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  792

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  793
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  793
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  794
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  794

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  795
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  795
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  796
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  796

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  797
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  797
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  798
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  798

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  799
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  799
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  800
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  800

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  801
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  801
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  802
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  802

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  803
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  803
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  804
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  804

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  805
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  805
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  806
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  806

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  807
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  807
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  808
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  808

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  809
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  809
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcacg tcgcctgcag      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  810
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  810

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Gln Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  811
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  811
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  812
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  812

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  813
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  813
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  814
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  814

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  815
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  815
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  816
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  816

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  817
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  817
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa atgcgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  818
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  818

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Cys Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  819
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  819
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cggcggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  820
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  820

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gly Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  821
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  821
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagcgta gctgcccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  822
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  822

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Arg Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  823
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  823
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  824
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  824

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  825
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  825
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

accgaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  826
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  826

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Thr Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  827
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  827
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtactgaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  828
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  828

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Leu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  829
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  829
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctaaa ccgcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  830
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  830

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Lys Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  831
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  831
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagatgc      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  832
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  832

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  833
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  833
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  834
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  834

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  835
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  835
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  836
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  836

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  837
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  837
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  838
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  838

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  839
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  839
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  840
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  840

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  841
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  841
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  842
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  842

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  843
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  843
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  844
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  844

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  845
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  845
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtggg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  846
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  846

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Trp Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  847
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  847
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgcagaact tatctgcaaa gcgcctggcg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  848
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  848

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  849
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  849
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  850
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  850

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  851
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  851
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtactgaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  852
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  852

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  853
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  853
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctgcag      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  854
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  854

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Gln Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  855
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  855
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  856
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  856

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  857
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  857
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  858
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  858

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  859
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  859
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  860
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  860

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  861
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  861
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  862
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  862

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  863
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  863
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccagt aa                                                          672


<210>  864
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  864

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Gln 
    210                 215                 220             


<210>  865
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  865
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa atgcgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  866
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  866

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Cys Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  867
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  867
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  868
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  868

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  869
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  869
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  870
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  870

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  871
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  871
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  872
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  872

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  873
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  873
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctgcg ccgcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  874
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  874

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Ala Pro Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  875
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  875
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  876
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  876

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  877
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  877
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctgcg ccgcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  878
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  878

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Ala Pro Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  879
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  879
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  880
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  880

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  881
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  881
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgcagaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  882
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  882

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  883
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  883
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcatg tcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  884
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  884

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Cys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  885
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  885
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc atcgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  886
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  886

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  887
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  887
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcacg tcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  888
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  888

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Arg Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  889
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  889
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccagt aa                                                          672


<210>  890
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  890

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Gln 
    210                 215                 220             


<210>  891
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  891
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  892
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  892

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  893
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  893
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagcgta gctgcccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  894
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  894

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Arg Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  895
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  895
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  896
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  896

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  897
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  897
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagcgta gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  898
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  898

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Arg Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  899
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  899
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcacg tcgcctgcag      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgtgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  900
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  900

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Gln Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Val Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  901
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  901
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  902
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  902

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  903
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris


<220>
<221>  unsure
<222>  (49)..(49)
<223>  n = a, c, g or t

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  n is a, c, g, or t

<400>  903
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtnt taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  904
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris


<220>
<221>  unsure
<222>  (17)..(17)
<223>  Xaa = Phe, Ile, Leu, Val

<400>  904

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Xaa Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  905
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  905
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  906
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  906

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  907
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  907
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgtggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  908
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  908

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  909
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  909
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatggcagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  910
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  910

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  911
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  911
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagtg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  912
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  912

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  913
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  913
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  914
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  914

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  915
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  915
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  916
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  916

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  917
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  917
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  918
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  918

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  919
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  919
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  920
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  920

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  921
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  921
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atttacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  922
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  922

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Phe Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  923
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  923
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  924
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  924

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  925
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  925
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagcgta gctgcccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  926
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  926

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Arg Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  927
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  927
atgggtagct ttggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  928
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  928

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  929
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  929
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  930
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  930

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  931
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  931
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  932
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  932

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  933
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  933
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  934
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  934

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  935
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  935
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  936
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  936

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  937
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  937
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  938
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  938

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  939
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  939
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  940
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  940

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  941
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  941
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  942
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  942

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  943
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  943
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  944
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  944

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  945
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  945
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  946
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  946

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  947
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  947
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  948
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  948

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  949
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  949
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgccgct aa                                                          672


<210>  950
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  950

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  951
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  951
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  952
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  952

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  953
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  953
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  954
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  954

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  955
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  955
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  956
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  956

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  957
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  957
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  958
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  958

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  959
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  959
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgct aa                                                          672


<210>  960
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  960

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  961
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  961
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  962
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  962

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  963
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  963
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcgtaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  964
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  964

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  965
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  965
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  966
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  966

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  967
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  967
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  968
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  968

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  969
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  969
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  970
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  970

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  971
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  971
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtactgaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  972
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  972

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Leu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  973
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  973
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  974
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  974

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  975
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  975
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  976
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  976

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  977
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  977
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  978
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  978

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  979
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  979
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  980
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  980

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  981
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  981
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atttacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  982
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  982

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Phe Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  983
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  983
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaacg tccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  984
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  984

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  985
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  985
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  986
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  986

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  987
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  987
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  988
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  988

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  989
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  989
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  990
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  990

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  991
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  991
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  992
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  992

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  993
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  993
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  994
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  994

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  995
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  995
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgaaaaact tagcggcacg tcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atttacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  996
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  996

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ala Ala Arg Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Phe Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  997
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  997
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  998
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  998

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  999
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  999
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1000
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1000

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1001
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1001
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgaaaaact tagcggcatg ccgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1002
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1002

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ala Ala Cys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1003
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1003
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg ccgaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1004
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1004

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1005
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1005
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atttacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1006
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1006

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Phe Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1007
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1007
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1008
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1008

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1009
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1009
atgcgtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga acacagatga agtgggaaca gcggagtacg gagccgaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgctggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1010
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1010

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Leu Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1011
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1011
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1012
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1012

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1013
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1013
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1014
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1014

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1015
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1015
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1016
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1016

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1017
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1017
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1018
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1018

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1019
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1019
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1020
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1020

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1021
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1021
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1022
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1022

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1023
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1023
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1024
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1024

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1025
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1025
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1026
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1026

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1027
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1027
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgctgccgct aa                                                          672


<210>  1028
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1028

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys Cys Arg 
    210                 215                 220             


<210>  1029
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1029
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1030
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1030

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1031
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1031
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1032
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1032

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1033
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1033
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1034
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1034

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1035
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1035
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1036
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1036

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1037
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1037
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc gtacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1038
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1038

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1039
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1039
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  1040
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1040

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  1041
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1041
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1042
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1042

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1043
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1043
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1044
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1044

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1045
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1045
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1046
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1046

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1047
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1047
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1048
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1048

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1049
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1049
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1050
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1050

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1051
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1051
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaacc atgcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgcccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1052
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1052

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn His Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1053
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1053
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gcggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgctgctgct aa                                                          672


<210>  1054
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1054

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys Cys Cys 
    210                 215                 220             


<210>  1055
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1055
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1056
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1056

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1057
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1057
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1058
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1058

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1059
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1059
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1060
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1060

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1061
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1061
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

tgctgccgct aa                                                          672


<210>  1062
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1062

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Cys Cys Arg 
    210                 215                 220             


<210>  1063
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1063
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcgtaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1064
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1064

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1065
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1065
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

tgctgccgct aa                                                          672


<210>  1066
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1066

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Cys Cys Arg 
    210                 215                 220             


<210>  1067
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1067
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1068
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1068

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1069
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1069
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1070
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1070

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1071
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1071
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1072
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1072

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1073
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1073
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1074
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1074

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1075
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1075
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1076
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1076

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1077
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1077
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtat ttggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1078
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1078

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Phe Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1079
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1079
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1080
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1080

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1081
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1081
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1082
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1082

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1083
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1083
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1084
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1084

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1085
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1085
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1086
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1086

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1087
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1087
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtat ttggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gcttgccgct aa                                                          672


<210>  1088
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1088

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Phe Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala Cys Arg 
    210                 215                 220             


<210>  1089
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1089
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1090
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1090

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1091
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1091
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtaaaaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1092
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1092

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1093
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1093
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gcttgccgct aa                                                          672


<210>  1094
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1094

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala Cys Arg 
    210                 215                 220             


<210>  1095
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1095
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

tgccaccgct aa                                                          672


<210>  1096
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1096

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Cys His Arg 
    210                 215                 220             


<210>  1097
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1097
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1098
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1098

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1099
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1099
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acggcgatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1100
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1100

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1101
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1101
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1102
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1102

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1103
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1103
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1104
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1104

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1105
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1105
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1106
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1106

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1107
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1107
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1108
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1108

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1109
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1109
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

tgctgccgct aa                                                          672


<210>  1110
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1110

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Cys Cys Arg 
    210                 215                 220             


<210>  1111
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1111
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1112
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1112

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1113
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1113
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1114
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1114

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1115
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1115
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1116
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1116

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1117
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1117
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1118
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1118

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1119
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1119
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagatgc      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gcttgccgct aa                                                          672


<210>  1120
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1120

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala Cys Arg 
    210                 215                 220             


<210>  1121
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1121
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1122
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1122

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1123
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1123
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1124
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1124

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1125
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1125
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1126
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1126

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1127
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1127
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1128
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1128

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1129
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1129
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1130
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1130

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1131
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1131
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1132
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1132

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1133
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1133
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa aaccgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gcttgccgct aa                                                          672


<210>  1134
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1134

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala Cys Arg 
    210                 215                 220             


<210>  1135
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1135
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1136
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1136

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1137
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1137
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtggccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1138
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1138

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Trp Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1139
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1139
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1140
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1140

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1141
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1141
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1142
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1142

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1143
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1143
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1144
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1144

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1145
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1145
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1146
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1146

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1147
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1147
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcgtaact tatctgcacg tcgcctgctg      120

acggcgatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1148
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1148

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Arg 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1149
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1149
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgaaaaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1150
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1150

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Lys 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1151
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1151
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1152
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1152

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1153
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1153
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc tgtttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1154
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1154

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Leu 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1155
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1155
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acggcgcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggcgtt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1156
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1156

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Ala 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1157
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1157
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1158
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1158

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1159
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1159
atgggtagca tgggaccggg aattggacca gacgaagctt taggccgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgctggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1160
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1160

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gly Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Leu Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1161
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1161
atgggtagca tgggaccggg aattggacca gacgaagctt taggccgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1162
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1162

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gly Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1163
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1163
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccactgct aa                                                          672


<210>  1164
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1164

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Cys 
    210                 215                 220             


<210>  1165
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1165
atgcgtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc atacagatga agtgggaaca gcggagtacg gagtggaatt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1166
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1166

Met Arg Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala His Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1167
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1167
atgggtagca tgggaccggg aattggacca gacgaagctt taggccgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1168
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1168

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gly Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1169
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1169
atgcgtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1170
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1170

Met Arg Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1171
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1171
atgggtagca tgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtaaaaact tagcggcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagcga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta ctggcgccga ttgtgcctgc agtacaggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcagt acacatctgg acctgctaaa      660

tgccaccgct aa                                                          672


<210>  1172
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1172

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Lys 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  1173
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1173
atgggcagca ccggcccggg cattggcccg gacgaagcct tacagagatt gaaagagggg       60

aatgcgcgct tcgtggccga gactccaaga cctcagaacc tttctcttcg gcggcttctg      120

acctctatgc atggacaaac cccttatgct acaattctga gttgctcgga tagtcgtgct      180

ccggtggagt taatattcga tgcaggcata ggcgaccttt tcgtgattag agtggcaggg      240

aacgtagcgc agacagatga ggttgggaca gcagaatatg gtgtggatca ccttaatgtg      300

ccgctgctgg tggtaatggg gcatactcag tgcggggccg tcacagcggt tgttatgcgt      360

gcagaagtcc gcgggagcat tcctatgctg gttgcgccta tagttcctgc cgttatggcc      420

gttgagaaaa gttttccgaa aacagaccgt gccgcgctcg tccctaaagc gatagaggcc      480

aacgtgtggc aggctatcga cgacactttc cgccaatcgc cgataataag ggcgagggtc      540

gcagctggaa aacttaaggt cgtaggagca atttaccata ttgacgacgg gaaagttgaa      600

tggctcggtg aacaccccat gcaagcacga cttcttgaat ataccagcgg cccgaccaaa      660

gcgcatcgtt aa                                                          672


<210>  1174
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1174

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1175
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1175
atgggcagca ccggcccggg cattggcccg gacgaagcct tacagagatt gaaagagggg       60

aatgcgcgct tcgtggccga gactccaaga cctcagaacc tttctgctcg gcggcttctg      120

acctctatgc atggacaaac cccttatgct acaattctga gttgctcgga tagtcgtgct      180

ccggtggagt taatattcga tgcaggcata ggcgaccttt tcgtgattag agtggcaggg      240

aacgtagcgc agacagatga ggttgggaca gcagaatatg gtgtggatca ccttaatgtg      300

ccgctgctgg tggtaatggg gcatactcag tgcggggccg tcacagcggt tgttatgcgt      360

gcagaagtcc gcgggagcat tccttttctg gttgcgccta tagttcctgc cgttatggcc      420

gttgagaaaa gttttccgaa aacagaccgt gccgcgctcg tccctaaagc gatagaggcc      480

aacgtgtggc aggctatcga cgacactttc cgccaatcgc cgataataag ggcgagggtc      540

gcagctggaa aacttaaggt cgtaggagca atttaccata ttgacgacgg gaaagttgaa      600

tggctcggtg aacaccccat gcaagcacga cttcttgaat ataccagcgg cccgaccaaa      660

gcgcatcgtt aa                                                          672


<210>  1176
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1176

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1177
<211>  675
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1177
atgggcagca ccggcccggg cattggcccg gacgaagcct tacagagatt gaaagagggg       60

aatgcgcgct tcgtggccga gactccaaga cctcagaacc tttctgctcg gcggcttctg      120

acctctatgc atggacaaac cccttatgct acaattctga gttgctcgga tagtcgtgct      180

ccggtggagt taatatatga tgcaggcata ggcgaccttt tcgtgattag agtggcaggg      240

aacgtagcgc agacagatga ggttgggaca gcagaatatg gtgtggatca ccttaatgtg      300

ccgctgctgg tggtaatggg gcatactcag tgcggggccg tcacagcggt tgttatgcgt      360

gcagaagtcc gcgggagcat tcctatgctg gttgcgccta tagttcctgc cgttatggcc      420

gttgagaaaa gttttccgaa aacagaccgt gccgcgctcg tccctaaagc gatagaggcc      480

aacgtgtggc aggctatcga cgacactttc cgccaatcgc cgataataag ggcgagggtc      540

gcagctggaa aacttaaggt cgtaggagca atttaccata ttgacgacgg gaaagttgaa      600

tggctcggtg aacaccccat gcaagcacga cttcttgaat ataccagcgg cccgaccaaa      660

gcgcatcgtt aatga                                                       675


<210>  1178
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1178

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Tyr Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1179
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1179
atgggcagca ccggcccggg cattggcccg gacgaagcct tacagagatt gaaagagggg       60

aatgcgcgct tcgtggccga gactccaaga cctcagaacc tttctgctcg gcggcttctg      120

acctctatgc atggacaaac cccttatgct acaattctga gttgctcgga tagtcgtgct      180

ccggtggagt taatattcga tgcaggcata ggcgaccttt tcgtgattag agtggcaggg      240

aacgtagcgc agacagatga ggttgggaca gcagaatatg gtgtggatca ccttaatgtg      300

ccgctgctgg tggtaatggg gcatactcag tgcggggccg tcacagcggt tgttatgcgt      360

gcagaaaaac gcgggagcat tcctatgctg gttgcgccta tagttcctgc cgttatggcc      420

gttgagaaaa gttttccgaa aacagaccgt gccgcgctcg tccctaaagc gatagaggcc      480

aacgtgtggc aggctatcga cgacactttc cgccaatcgc cgataataag ggcgagggtc      540

gcagctggaa aacttaaggt cgtaggagca atttaccata ttgacgacgg gaaagttgaa      600

tggctcggtg aacaccccat gcaagcacga cttcttgaat ataccagcgg cccgaccaaa      660

gcgcatcgtt aa                                                          672


<210>  1180
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1180

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1181
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1181
atgggcagca ccggcccggg cattggcccg gacgaagcct tacagagatt gaaagagggg       60

aatgcgcgct tcgtggccga gactccaaga cctcagaacc tttctgctcg gcggcttctg      120

acctctatgc atggacaaac cccttatgct acaattctga gttgctcgga tagtcgtgct      180

ccggtggagt taatattcga tgcaggcata ggcgaccttt tcgtgattag agtggcaggg      240

aacgtagcgc agacagatga ggttgggaca gcagaatatg gtgtggatca ccttaatgtg      300

ccgctgctgg tggtaatggg gcatactcag tgcggggccg tcacagcggt tgttatgcgt      360

gcagaagtcc gcgggagcat tcctatgctg gttgcgccta tagttcctgc cgttatggcc      420

gttgagaaaa gttttccgaa aaaagaccgt gccgcgctcg tccctaaagc gatagaggcc      480

aacgtgtggc aggctatcga cgacactttc cgccaatcgc cgataataag ggcgagggtc      540

gcagctggaa aacttaaggt cgtaggagca atttaccata ttgacgacgg gaaagttgaa      600

tggctcggtg aacaccccat gcaagcacga cttcttgaat ataccagcgg cccgaccaaa      660

gcgcatcgtt aa                                                          672


<210>  1182
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1182

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Lys Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1183
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1183
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1184
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1184

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1185
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1185
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1186
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1186

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1187
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1187
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1188
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1188

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1189
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1189
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1190
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1190

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1191
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1191
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1192
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1192

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1193
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1193
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1194
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1194

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1195
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1195
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaaagaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1196
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1196

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Lys Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1197
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1197
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaaagaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1198
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1198

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Lys Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1199
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1199
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1200
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1200

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1201
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1201
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1202
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1202

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1203
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1203
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggagaaac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1204
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1204

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1205
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1205
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1206
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1206

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1207
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1207
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1208
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1208

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1209
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1209
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1210
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1210

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1211
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1211
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1212
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1212

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1213
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1213
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1214
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1214

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1215
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1215
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1216
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1216

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1217
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1217
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1218
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1218

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1219
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1219
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1220
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1220

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1221
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1221
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1222
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1222

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1223
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1223
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1224
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1224

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1225
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1225
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1226
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1226

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1227
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1227
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaaagaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1228
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1228

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Lys Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1229
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1229
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1230
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1230

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1231
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1231
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1232
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1232

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1233
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1233
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1234
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1234

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1235
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1235
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1236
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1236

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1237
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1237
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1238
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1238

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1239
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1239
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1240
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1240

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1241
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1241
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1242
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1242

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1243
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1243
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1244
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1244

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1245
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1245
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggagaaac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1246
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1246

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1247
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1247
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctctgcg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1248
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1248

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Leu Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1249
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1249
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggagaaac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctttccgaa aaaagaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1250
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1250

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys Lys Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1251
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1251
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1252
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1252

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1253
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1253
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1254
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1254

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1255
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1255
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggagaaac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttttccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1256
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1256

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Phe Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1257
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1257
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggagaaac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1258
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1258

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1259
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1259
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggagaaac gtggatcaat cccgtattta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1260
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1260

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Tyr Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1261
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1261
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggagaaac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aattgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1262
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1262

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Ile Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1263
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1263
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggagaaac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1264
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1264

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1265
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1265
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gctgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1266
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1266

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1267
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1267
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagcgga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggagaaac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1268
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1268

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1269
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1269
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcgt      360

gcggagaaac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1270
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1270

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Lys Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1271
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1271
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtttgcc acggtgcttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga ccagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggaaacat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1272
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1272

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Phe Ala Thr Val Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gln Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Asn Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1273
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1273
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacaccgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1274
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1274

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Thr Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1275
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1275
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga ccagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggaaacat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1276
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1276

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gln Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Asn Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1277
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1277
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtttgcc acggtgcttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga ccagggcatt ggcgatctat tcgttgtgcg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggaaacat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1278
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1278

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Phe Ala Thr Val Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gln Gly Ile Gly Asp Leu Phe Val Val Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Asn Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1279
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1279
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acggtgcttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggaaacat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1280
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1280

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Val Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Asn Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1281
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1281
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggaaca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggatcaat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1282
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1282

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1283
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1283
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtttgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga ccagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggaaacat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1284
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1284

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Phe Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gln Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Asn Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1285
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1285
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcacg tcgcctgctg      120

acgtctatgc tgggacaaac gccgtttgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgcgggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagtggacca ccttaacgtt      300

cctttgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac gtggaaacat cccgttttta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaagc gttgcccgaa aaccgaccgc gcggctttag tgccgaaggc gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctaccaaa      660

gctcaccgct aa                                                          672


<210>  1286
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  1286

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Leu Thr Ser Met Leu Gly Gln Thr Pro 
        35                  40                  45              


Phe Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val Arg Gly Asn Ile Pro 
        115                 120                 125             


Phe Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Arg 
    130                 135                 140                 


Cys Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  1287
<211>  714
<212>  DNA
<213>  Desulfovibrio sp. FW1012B

<400>  1287
atgaaacggt ttcttgcggc cacggccacc atggcgtttt tgctggcgat gtgcacagcg       60

gtcctggcct cttccggcgg ttcggaagtg agcgccgacg aggccctgtc ccggctcaag      120

gaaggcaaca cgcggttcgt gtcccaggcc aacgtcgcgc cgcatcagga cgccgcccgt      180

cggcatgaaa ccgccaccgg cggacagcac ccgtttgcca cggtcctgtc ctgcgccgat      240

tcccgggccc cggtcgaggt cctcttcgac cagggcgtgg gcgacctttt cgtggtccgc      300

gtggccggca acgtggccgc caccgacgag atcggcacca tcgagtacgg ggccgagcac      360

ctcggcgtgc cgttggtggt ggttctggcc cacaccaaat gcggggcggt cacggcggtg      420

gtcaaaaacg agcccgtcac cgagaacatc ggcaagctgg tggcccccat cgtgccggcg      480

gtcaagggca tcaaggcccg tttcgccgct tcggacgtca acgagatcat cagccggtcc      540

atcgaggcca acatgtggca ggccatcagc gacatctacg ccaaaagccc gatgctcaaa      600

aagatggcgg ccgacggcaa gatcaaggtc gtgggcgccc tctacgacat cgactccggc      660

gaagtgcgct ggttcggcga gcacccgtcc gaaggctccc tgctcgacaa ttaa            714


<210>  1288
<211>  237
<212>  PRT
<213>  Desulfovibrio sp. FW1012B

<400>  1288

Met Lys Arg Phe Leu Ala Ala Thr Ala Thr Met Ala Phe Leu Leu Ala 
1               5                   10                  15      


Met Cys Thr Ala Val Leu Ala Ser Ser Gly Gly Ser Glu Val Ser Ala 
            20                  25                  30          


Asp Glu Ala Leu Ser Arg Leu Lys Glu Gly Asn Thr Arg Phe Val Ser 
        35                  40                  45              


Gln Ala Asn Val Ala Pro His Gln Asp Ala Ala Arg Arg His Glu Thr 
    50                  55                  60                  


Ala Thr Gly Gly Gln His Pro Phe Ala Thr Val Leu Ser Cys Ala Asp 
65                  70                  75                  80  


Ser Arg Ala Pro Val Glu Val Leu Phe Asp Gln Gly Val Gly Asp Leu 
                85                  90                  95      


Phe Val Val Arg Val Ala Gly Asn Val Ala Ala Thr Asp Glu Ile Gly 
            100                 105                 110         


Thr Ile Glu Tyr Gly Ala Glu His Leu Gly Val Pro Leu Val Val Val 
        115                 120                 125             


Leu Ala His Thr Lys Cys Gly Ala Val Thr Ala Val Val Lys Asn Glu 
    130                 135                 140                 


Pro Val Thr Glu Asn Ile Gly Lys Leu Val Ala Pro Ile Val Pro Ala 
145                 150                 155                 160 


Val Lys Gly Ile Lys Ala Arg Phe Ala Ala Ser Asp Val Asn Glu Ile 
                165                 170                 175     


Ile Ser Arg Ser Ile Glu Ala Asn Met Trp Gln Ala Ile Ser Asp Ile 
            180                 185                 190         


Tyr Ala Lys Ser Pro Met Leu Lys Lys Met Ala Ala Asp Gly Lys Ile 
        195                 200                 205             


Lys Val Val Gly Ala Leu Tyr Asp Ile Asp Ser Gly Glu Val Arg Trp 
    210                 215                 220                 


Phe Gly Glu His Pro Ser Glu Gly Ser Leu Leu Asp Asn 
225                 230                 235         


<210>  1289
<211>  978
<212>  DNA
<213>  Desulfomicrobium baculatum (strain DSM 4028)

<400>  1289
atgttcatca aacgtcttgt cctggtcttt ctgcttgtct ttgctccgct ttcggtcatg       60

gcgtcatctc ctggcgctca tcccgtaagt cccgaaaatg gattgaagat gctcaccgaa      120

ggaaacctgc ggttcgccct gggccaatcg acccacccca acaccagttt ttccaggcgt      180

ctgctaacca ccacggaagg acaggccccc ttcgctacgg tcatcgcctg ctccgattcg      240

agagtcccgg ttgaaatcct tttcgatcaa gggattggag acttgtttgt gatcaaggtc      300

gccggcaacg tggccgacac ggacgaaatc gggtccgccg aatatggcgt ggaccatctc      360

ggcacgccgg tgctcatggt gctcggacac tcctactgcg gcgccgtcac ggccgtgacc      420

acgggggccg aggtccatgg cagcattccc gccctggtcg acaacatcgt gcctgcggtg      480

gataaggctc gccatgatca cccggatgcc gaaactcccg aactcatcgt ccaggccatc      540

gagaccaatg tctggcaggc catcgaagac ctgctcgaca ccagccacgc tatcgccgac      600

cgggccaagg acggccgggt tatcgtgatt ggagccgtgt acgacatcct caccggcaag      660

gtgaacattc tgggcgctca tcccaaccag accgagctcc tgggcggagt gacgcccccg      720

gtccacgccg aacccgccat gcacgcagaa ccggcaaagg atgcccacgc tccggccgag      780

caggcgccag ccgaacaggc ggacgccgcc catgcggacc cgaccgtgga aaaggccgcg      840

gccgaagggc atgccgaagc ggcggaagcc ccttcctccg gagggttcgg ctttttctcc      900

ttcatcgtgt tcgttcttct gctcattgga gcggtcttcg tgctcgacaa gaagatactc      960

aatcccgacc aggactaa                                                    978


<210>  1290
<211>  325
<212>  PRT
<213>  Desulfomicrobium baculatum (strain DSM 4028)

<400>  1290

Met Phe Ile Lys Arg Leu Val Leu Val Phe Leu Leu Val Phe Ala Pro 
1               5                   10                  15      


Leu Ser Val Met Ala Ser Ser Pro Gly Ala His Pro Val Ser Pro Glu 
            20                  25                  30          


Asn Gly Leu Lys Met Leu Thr Glu Gly Asn Leu Arg Phe Ala Leu Gly 
        35                  40                  45              


Gln Ser Thr His Pro Asn Thr Ser Phe Ser Arg Arg Leu Leu Thr Thr 
    50                  55                  60                  


Thr Glu Gly Gln Ala Pro Phe Ala Thr Val Ile Ala Cys Ser Asp Ser 
65                  70                  75                  80  


Arg Val Pro Val Glu Ile Leu Phe Asp Gln Gly Ile Gly Asp Leu Phe 
                85                  90                  95      


Val Ile Lys Val Ala Gly Asn Val Ala Asp Thr Asp Glu Ile Gly Ser 
            100                 105                 110         


Ala Glu Tyr Gly Val Asp His Leu Gly Thr Pro Val Leu Met Val Leu 
        115                 120                 125             


Gly His Ser Tyr Cys Gly Ala Val Thr Ala Val Thr Thr Gly Ala Glu 
    130                 135                 140                 


Val His Gly Ser Ile Pro Ala Leu Val Asp Asn Ile Val Pro Ala Val 
145                 150                 155                 160 


Asp Lys Ala Arg His Asp His Pro Asp Ala Glu Thr Pro Glu Leu Ile 
                165                 170                 175     


Val Gln Ala Ile Glu Thr Asn Val Trp Gln Ala Ile Glu Asp Leu Leu 
            180                 185                 190         


Asp Thr Ser His Ala Ile Ala Asp Arg Ala Lys Asp Gly Arg Val Ile 
        195                 200                 205             


Val Ile Gly Ala Val Tyr Asp Ile Leu Thr Gly Lys Val Asn Ile Leu 
    210                 215                 220                 


Gly Ala His Pro Asn Gln Thr Glu Leu Leu Gly Gly Val Thr Pro Pro 
225                 230                 235                 240 


Val His Ala Glu Pro Ala Met His Ala Glu Pro Ala Lys Asp Ala His 
                245                 250                 255     


Ala Pro Ala Glu Gln Ala Pro Ala Glu Gln Ala Asp Ala Ala His Ala 
            260                 265                 270         


Asp Pro Thr Val Glu Lys Ala Ala Ala Glu Gly His Ala Glu Ala Ala 
        275                 280                 285             


Glu Ala Pro Ser Ser Gly Gly Phe Gly Phe Phe Ser Phe Ile Val Phe 
    290                 295                 300                 


Val Leu Leu Leu Ile Gly Ala Val Phe Val Leu Asp Lys Lys Ile Leu 
305                 310                 315                 320 


Asn Pro Asp Gln Asp 
                325 


<210>  1291
<211>  786
<212>  DNA
<213>  Desulfovibrio aespoeensis

<400>  1291
atggaccgta aattccattc cctggccatc atctgcgcga acgttgtgtt tgccactgtc       60

ctggtggcct tggcccaggc cgagctggtg gtgggcaaag cctcacccga cgaggtcatg      120

gccatgctca aggaaggcaa cgagcggttc gtcgccggga aggcgctcca tgaaaacacc      180

gacgcggagc ggctggccca ggccgggaac gagagccagg ccgatcatgc ctttgccacc      240

gtgctttcct gcgcggattc ccgcgtgccg gtggagcgga ttttcgacgc cggattcata      300

gacctgttcg tggtccgggt ggcgggcaat gtggtgaaaa ccgacgaggc cgggtccatc      360

gagtacgggc tggcccatgt gcacacgccg gtgctggtgg tcatggggca cacccaatgc      420

ggcgctgtcg cagccgtgac cgacgtggtg caggggcgca agctgacgtt tgaacgcaat      480

attccgccgc tggtcgctcc catcatcccg gctgtgcagc gggccatcgc cgcccatccc      540

gatgcttcgg gcagggccgt gttgcccttt gccatcgagg agaacgtctg gcagggcatc      600

acggacctgt tcatgcagag cccggccacc cgcatgatcg tcaggtccgg caaggtcaag      660

gtggtcgggg cgatttacga cgtcgggact ggccgcgtca gttggctgcc cgagtccagg      720

gttctcgaac tgctggacgc ggtggagcgc gaccctggcc gtgccatgaa cgccatggcc      780

gagtag                                                                 786


<210>  1292
<211>  261
<212>  PRT
<213>  Desulfovibrio aespoeensis

<400>  1292

Met Asp Arg Lys Phe His Ser Leu Ala Ile Ile Cys Ala Asn Val Val 
1               5                   10                  15      


Phe Ala Thr Val Leu Val Ala Leu Ala Gln Ala Glu Leu Val Val Gly 
            20                  25                  30          


Lys Ala Ser Pro Asp Glu Val Met Ala Met Leu Lys Glu Gly Asn Glu 
        35                  40                  45              


Arg Phe Val Ala Gly Lys Ala Leu His Glu Asn Thr Asp Ala Glu Arg 
    50                  55                  60                  


Leu Ala Gln Ala Gly Asn Glu Ser Gln Ala Asp His Ala Phe Ala Thr 
65                  70                  75                  80  


Val Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Arg Ile Phe Asp 
                85                  90                  95      


Ala Gly Phe Ile Asp Leu Phe Val Val Arg Val Ala Gly Asn Val Val 
            100                 105                 110         


Lys Thr Asp Glu Ala Gly Ser Ile Glu Tyr Gly Leu Ala His Val His 
        115                 120                 125             


Thr Pro Val Leu Val Val Met Gly His Thr Gln Cys Gly Ala Val Ala 
    130                 135                 140                 


Ala Val Thr Asp Val Val Gln Gly Arg Lys Leu Thr Phe Glu Arg Asn 
145                 150                 155                 160 


Ile Pro Pro Leu Val Ala Pro Ile Ile Pro Ala Val Gln Arg Ala Ile 
                165                 170                 175     


Ala Ala His Pro Asp Ala Ser Gly Arg Ala Val Leu Pro Phe Ala Ile 
            180                 185                 190         


Glu Glu Asn Val Trp Gln Gly Ile Thr Asp Leu Phe Met Gln Ser Pro 
        195                 200                 205             


Ala Thr Arg Met Ile Val Arg Ser Gly Lys Val Lys Val Val Gly Ala 
    210                 215                 220                 


Ile Tyr Asp Val Gly Thr Gly Arg Val Ser Trp Leu Pro Glu Ser Arg 
225                 230                 235                 240 


Val Leu Glu Leu Leu Asp Ala Val Glu Arg Asp Pro Gly Arg Ala Met 
                245                 250                 255     


Asn Ala Met Ala Glu 
            260     


<210>  1293
<211>  1200
<212>  DNA
<213>  Desulfovibrio desulfuricans (strain G20)

<400>  1293
atgattttca aatcgcggaa aaccctgctg gctgcccttg gactggtggc tttgtttctt       60

gttttcggtg cgttttcgtg cagcagtgac agcacggtgg aacgtacgcc cgaagctgcg      120

ctgaaagccc tcagacaggg caacgaacgt tttgcagccg gcgaagctgt gcacccccgg      180

actgatgccg cgcgtctggc gcaggccggc acggaaagtc agggcgatca tgcatacgcc      240

acagtgctgg cctgttctga ctcacgcgtg cccgtggaac gcctgtttga tgccggagtc      300

atggatattt ttgtggtgcg tgtggccggc aacgtagtgc agggcgacga agccggttcc      360

attgaatatg gtctggccca tgtgaaaacc cctgtgctgg tggtgctggg acatacccag      420

tgcggtgccg tgaccgccgt gaccgcagcg ctggaaggac acggtcatgc gctggaacgc      480

aatatccccg gtctggtcaa gcccgtcatt cctgccgtgc agcaggccat gcaggaacac      540

cccgatgtgc acggtgcgga tctggtgccc ttcggcattg aaaataatgt ctggcagaac      600

atccgtaatc ttttcatgct gagccccgcc accagagatc tggtaaaatc cggcaaggtg      660

gctgttgtcg gagccgtata cgatgtgtcc accggcaagg ttgaatggct gcccgccgcc      720

cgcgtggatg aaattcttgc cgcagtggaa gcagaccccg cccgtgcgac ggaagccatg      780

gcttccgggg ccggcgccgc acagccggac catgacggcg cgcccgctgc tgcagcagaa      840

aatgaacagg ccgtatccga agctgaacac gctgctgatg ctgcagaaga cgccgccgga      900

tcttccggtt ccggcgcaga ggttgaaacc gttgaagccg ttgaggttcc cgcggccaac      960

gccacagacg caggcgaaga gaccgtgacg caggaagccg cagtgccctg ccccggtcag     1020

atggcccgcg acaccatggc cattcatgca caggctgcgc tggaacaggc tgaaatggcg     1080

ggcaaggccg cacaggttgc cgccgaagct gccgcaaagg ctgccgaggc cgcgggagaa     1140

gccgcccgtg ccgccgaagc cgctgcactg gcggcgcagg ccgctcaggc gcagcagtag     1200


<210>  1294
<211>  399
<212>  PRT
<213>  Desulfovibrio desulfuricans (strain G20)

<400>  1294

Met Ile Phe Lys Ser Arg Lys Thr Leu Leu Ala Ala Leu Gly Leu Val 
1               5                   10                  15      


Ala Leu Phe Leu Val Phe Gly Ala Phe Ser Cys Ser Ser Asp Ser Thr 
            20                  25                  30          


Val Glu Arg Thr Pro Glu Ala Ala Leu Lys Ala Leu Arg Gln Gly Asn 
        35                  40                  45              


Glu Arg Phe Ala Ala Gly Glu Ala Val His Pro Arg Thr Asp Ala Ala 
    50                  55                  60                  


Arg Leu Ala Gln Ala Gly Thr Glu Ser Gln Gly Asp His Ala Tyr Ala 
65                  70                  75                  80  


Thr Val Leu Ala Cys Ser Asp Ser Arg Val Pro Val Glu Arg Leu Phe 
                85                  90                  95      


Asp Ala Gly Val Met Asp Ile Phe Val Val Arg Val Ala Gly Asn Val 
            100                 105                 110         


Val Gln Gly Asp Glu Ala Gly Ser Ile Glu Tyr Gly Leu Ala His Val 
        115                 120                 125             


Lys Thr Pro Val Leu Val Val Leu Gly His Thr Gln Cys Gly Ala Val 
    130                 135                 140                 


Thr Ala Val Thr Ala Ala Leu Glu Gly His Gly His Ala Leu Glu Arg 
145                 150                 155                 160 


Asn Ile Pro Gly Leu Val Lys Pro Val Ile Pro Ala Val Gln Gln Ala 
                165                 170                 175     


Met Gln Glu His Pro Asp Val His Gly Ala Asp Leu Val Pro Phe Gly 
            180                 185                 190         


Ile Glu Asn Asn Val Trp Gln Asn Ile Arg Asn Leu Phe Met Leu Ser 
        195                 200                 205             


Pro Ala Thr Arg Asp Leu Val Lys Ser Gly Lys Val Ala Val Val Gly 
    210                 215                 220                 


Ala Val Tyr Asp Val Ser Thr Gly Lys Val Glu Trp Leu Pro Ala Ala 
225                 230                 235                 240 


Arg Val Asp Glu Ile Leu Ala Ala Val Glu Ala Asp Pro Ala Arg Ala 
                245                 250                 255     


Thr Glu Ala Met Ala Ser Gly Ala Gly Ala Ala Gln Pro Asp His Asp 
            260                 265                 270         


Gly Ala Pro Ala Ala Ala Ala Glu Asn Glu Gln Ala Val Ser Glu Ala 
        275                 280                 285             


Glu His Ala Ala Asp Ala Ala Glu Asp Ala Ala Gly Ser Ser Gly Ser 
    290                 295                 300                 


Gly Ala Glu Val Glu Thr Val Glu Ala Val Glu Val Pro Ala Ala Asn 
305                 310                 315                 320 


Ala Thr Asp Ala Gly Glu Glu Thr Val Thr Gln Glu Ala Ala Val Pro 
                325                 330                 335     


Cys Pro Gly Gln Met Ala Arg Asp Thr Met Ala Ile His Ala Gln Ala 
            340                 345                 350         


Ala Leu Glu Gln Ala Glu Met Ala Gly Lys Ala Ala Gln Val Ala Ala 
        355                 360                 365             


Glu Ala Ala Ala Lys Ala Ala Glu Ala Ala Gly Glu Ala Ala Arg Ala 
    370                 375                 380                 


Ala Glu Ala Ala Ala Leu Ala Ala Gln Ala Ala Gln Ala Gln Gln 
385                 390                 395                 


<210>  1295
<211>  714
<212>  DNA
<213>  Desulfovibrio magneticus (strain ATCC 700980)

<400>  1295
atgaaacggt tcgttgcggc gtttgcggga gcggtcatca cgttttccat ggctggggcg       60

gccatggcgt tttccggcgg ggcgggcatt tccgccgatg aggcgctggc ccgcctcaag      120

gaaggcaaca cccgctatgt ggccggcgcg gccgtgaccc cgcgtcagga cgcggcccgg      180

cggcatgaaa ccgccaccgg cggccagcat ccctttgtct cggtgctgtc ctgcgccgat      240

tcccgcgtgc ccgtggaaac cgtgttcgac cagggcattg gcgatgtgtt cgtgatccgc      300

gtggccggca acgtggccaa caccgacgag atcggtacca tcgaatacgg gaccgaacac      360

ctgggcaccc ccctggtggt ggtcctggcc cacaccaaat gcggcgcggt cacggccgtg      420

gtcaagggcg agcatgtcac cgaaaacatc ggcaagctcg tggcccccat cgtgccggcc      480

gtggcttcgg tgaaaagccg ctttgcctcg ggcgacttga acgagcttat caaccgctcc      540

atcgaggcca acatgtggca ggccatcgcc gacatgtacg ccaaaagccc cttgctcaag      600

aagatggcgg ccgacggcaa gatcaaggtt gtgggcgcgc tctatgacat cgattccggc      660

gatgtccatt ggtttggcga gcatccgtcc aacgccaacc ttatcggcaa gtag            714


<210>  1296
<211>  237
<212>  PRT
<213>  Desulfovibrio magneticus (strain ATCC 700980)

<400>  1296

Met Lys Arg Phe Val Ala Ala Phe Ala Gly Ala Val Ile Thr Phe Ser 
1               5                   10                  15      


Met Ala Gly Ala Ala Met Ala Phe Ser Gly Gly Ala Gly Ile Ser Ala 
            20                  25                  30          


Asp Glu Ala Leu Ala Arg Leu Lys Glu Gly Asn Thr Arg Tyr Val Ala 
        35                  40                  45              


Gly Ala Ala Val Thr Pro Arg Gln Asp Ala Ala Arg Arg His Glu Thr 
    50                  55                  60                  


Ala Thr Gly Gly Gln His Pro Phe Val Ser Val Leu Ser Cys Ala Asp 
65                  70                  75                  80  


Ser Arg Val Pro Val Glu Thr Val Phe Asp Gln Gly Ile Gly Asp Val 
                85                  90                  95      


Phe Val Ile Arg Val Ala Gly Asn Val Ala Asn Thr Asp Glu Ile Gly 
            100                 105                 110         


Thr Ile Glu Tyr Gly Thr Glu His Leu Gly Thr Pro Leu Val Val Val 
        115                 120                 125             


Leu Ala His Thr Lys Cys Gly Ala Val Thr Ala Val Val Lys Gly Glu 
    130                 135                 140                 


His Val Thr Glu Asn Ile Gly Lys Leu Val Ala Pro Ile Val Pro Ala 
145                 150                 155                 160 


Val Ala Ser Val Lys Ser Arg Phe Ala Ser Gly Asp Leu Asn Glu Leu 
                165                 170                 175     


Ile Asn Arg Ser Ile Glu Ala Asn Met Trp Gln Ala Ile Ala Asp Met 
            180                 185                 190         


Tyr Ala Lys Ser Pro Leu Leu Lys Lys Met Ala Ala Asp Gly Lys Ile 
        195                 200                 205             


Lys Val Val Gly Ala Leu Tyr Asp Ile Asp Ser Gly Asp Val His Trp 
    210                 215                 220                 


Phe Gly Glu His Pro Ser Asn Ala Asn Leu Ile Gly Lys 
225                 230                 235         


