                         SEQUENCE LISTING

<110>  Codexis, Inc.
       ALVIZO, Oscar
 
<120>  HIGHLY STABLE BETA-CLASS CARBONIC ANHYDRASES USEFUL IN CARBON 
       CAPTURE SYSTEMS

<130>  CX4-061USP1

<160>  278   

<170>  PatentIn version 3.5

<210>  1
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized carbonic anhydrase gene from Desulfovibrio 
       vulgaris

<400>  1
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  2
<211>  223
<212>  PRT
<213>  Desulfovibrio vulgaris str. "Miyazaki F"

<400>  2

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  3
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  3
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  4
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  4

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  5
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  5
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  6
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  6

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  7
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  7
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  8
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  8

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  9
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  9
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaaagg      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  10
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  10

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  11
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  11
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcaa cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  12
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  12

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  13
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  13
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  14
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  14

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  15
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  15
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  16
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  16

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  17
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  17
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  18
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  18

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  19
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  19
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  20
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  20

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  21
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  21
atgggtagca tgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctaaa cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  22
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  22

Met Gly Ser Met Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Lys Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  23
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  23
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  24
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  24

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  25
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  25
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  26
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  26

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  27
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  27
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtaaaaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  28
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  28

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Lys 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  29
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  29
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta cttgcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  30
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  30

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Leu Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  31
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  31
atgggtagca cgggaccggg aattggacca gacgaagctt taaggcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctagg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  32
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  32

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Arg Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  33
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  33
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctggccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  34
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  34

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Trp Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  35
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  35
atgggtagca cgggaccggg aattggacca gacgaagctt tacagagtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  36
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  36

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Ser 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  37
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  37
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgtgg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  38
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  38

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Trp Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  39
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  39
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactggaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  40
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  40

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Glu Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  41
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  41
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gcttgtcgct aa                                                          672


<210>  42
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  42

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala Cys Arg 
    210                 215                 220             


<210>  43
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  43
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtattgaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  44
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  44

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Leu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  45
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  45
atgacaagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  46
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  46

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  47
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  47
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccacagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  48
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  48

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  49
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  49
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagt tgaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  50
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  50

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Leu 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  51
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  51
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatccatgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  52
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  52

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile His Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  53
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  53
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  54
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  54

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Gln Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  55
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  55
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa aacagaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  56
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  56

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Thr Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  57
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  57
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacattt cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  58
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  58

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Phe Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  59
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  59
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagcaga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  60
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  60

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Ala Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  61
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  61
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

aaagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  62
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  62

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Lys Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  63
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  63
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctttccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  64
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  64

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Phe Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  65
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  65
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcgtaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  66
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  66

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Arg 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  67
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  67
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

tgggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  68
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  68

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Trp Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  69
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  69
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaag gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  70
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  70

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Arg Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  71
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  71
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

tgtcaccgct aa                                                          672


<210>  72
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  72

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Cys His Arg 
    210                 215                 220             


<210>  73
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  73
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga gtacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  74
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  74

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ser Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  75
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  75
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtacgt      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  76
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  76

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Arg Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  77
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  77
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaaaagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  78
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  78

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Lys Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  79
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  79
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagttgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  80
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  80

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Val Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  81
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  81
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga atacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  82
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  82

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Asn Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  83
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  83
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctatgc atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  84
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  84

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Met His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  85
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  85
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gttgaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  86
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  86

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Val Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  87
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  87
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccggg tcacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  88
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  88

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Gly His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  89
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  89
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcactgtt aa                                                          672


<210>  90
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  90

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Cys 
    210                 215                 220             


<210>  91
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  91
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctgca cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  92
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  92

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Ala Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  93
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  93
atgcgtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  94
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  94

Met Arg Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  95
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  95
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

catgaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  96
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  96

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly His Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  97
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  97
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

caagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  98
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  98

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Gln Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  99
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  99
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagatgt      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  100
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  100

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Cys Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  101
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  101
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagcctgtca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  102
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  102

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Cys 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  103
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  103
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctttg cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  104
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  104

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Leu Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  105
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  105
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgttg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  106
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  106

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  107
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  107
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgactt tcttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  108
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  108

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


Phe Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  109
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  109
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgcaggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  110
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  110

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Ala Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  111
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  111
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acggcacaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctactaaa      660

gctcaccgct aa                                                          672


<210>  112
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  112

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ala Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Thr Lys Ala His Arg 
    210                 215                 220             


<210>  113
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  113
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcatt ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  114
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  114

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Ile Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  115
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  115
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaacagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  116
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  116

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Thr Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  117
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  117
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  118
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  118

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Glu 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  119
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  119
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tagcagcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  120
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  120

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ala Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  121
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  121
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtag gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  122
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  122

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Gly Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  123
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  123
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaaaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  124
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  124

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Lys Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  125
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  125
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc attgacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  126
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  126

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Leu Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  127
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  127
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgaaacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  128
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  128

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Glu Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  129
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  129
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtacatgct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  130
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  130

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val His Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  131
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  131
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

acagaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  132
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  132

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Thr Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  133
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  133
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

ttggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  134
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  134

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Leu Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  135
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  135
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaagg caaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  136
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  136

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ala 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  137
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  137
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gctgtccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  138
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  138

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Cys Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  139
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  139
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgcaat acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  140
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  140

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Gln Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  141
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  141
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccaaaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  142
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  142

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Lys 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  143
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  143
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag aggtcgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  144
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  144

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Gly Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  145
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  145
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac gtggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  146
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  146

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val Arg Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  147
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  147
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgcaca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  148
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  148

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Ala 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  149
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  149
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tacgtgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  150
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  150

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Arg Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  151
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  151
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgttgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  152
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  152

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Val Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  153
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  153
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaag gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  154
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  154

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Arg Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  155
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  155
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa agcagaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  156
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  156

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys Ala Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  157
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  157
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga ggacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  158
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  158

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Arg Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  159
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  159
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctgttc atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  160
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  160

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Val His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  161
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  161
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatccgtgcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  162
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  162

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Arg Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  163
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  163
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgtt tcacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  164
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  164

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Phe His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  165
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  165
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cggtggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  166
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  166

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Gly Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  167
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  167
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggc aatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  168
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  168

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Ala Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  169
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  169
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atggacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  170
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  170

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Trp Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  171
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  171
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc atttacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  172
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  172

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Phe Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  173
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  173
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccaat aa                                                          672


<210>  174
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  174

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Gln 
    210                 215                 220             


<210>  175
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  175
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaga acaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  176
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  176

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Glu Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  177
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  177
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcga aaacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  178
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  178

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Lys Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  179
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  179
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagtt      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  180
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  180

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  181
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  181
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgcaa      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  182
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  182

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Gln Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  183
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  183
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatggtagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  184
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  184

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Gly Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  185
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  185
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtgcgga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagatga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaaa tcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  186
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  186

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ala Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Met Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Asn Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  187
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  187
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  188
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  188

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  189
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  189
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  190
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  190

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  191
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  191
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  192
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  192

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  193
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  193
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  194
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  194

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  195
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  195
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatggga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  196
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  196

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  197
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  197
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  198
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  198

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  199
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  199
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  200
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  200

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  201
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  201
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  202
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  202

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  203
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  203
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  204
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  204

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  205
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  205
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  206
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  206

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  207
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  207
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  208
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  208

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  209
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  209
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  210
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  210

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  211
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  211
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  212
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  212

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  213
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  213
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaacc attctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  214
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  214

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  215
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  215
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  216
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  216

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  217
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  217
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  218
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  218

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  219
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  219
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtaatgcgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  220
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  220

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Met Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  221
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  221
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  222
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  222

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  223
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  223
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  224
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  224

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  225
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  225
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  226
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  226

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  227
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  227
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  228
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  228

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  229
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  229
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  230
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  230

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  231
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  231
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  232
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  232

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  233
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  233
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  234
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  234

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  235
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  235
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  236
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  236

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  237
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  237
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  238
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  238

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  239
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  239
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  240
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  240

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  241
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  241
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  242
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  242

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  243
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  243
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  244
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  244

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  245
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  245
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  246
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  246

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  247
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  247
atgaccagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  248
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  248

Met Thr Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  249
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  249
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtactgcga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  250
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  250

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Leu Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  251
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  251
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  252
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  252

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  253
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  253
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgac ccacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  254
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  254

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Thr His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  255
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  255
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  256
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  256

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  257
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  257
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctgctg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  258
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  258

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Leu Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  259
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  259
atgggtagct ttggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcag cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  260
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  260

Met Gly Ser Phe Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Gln Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  261
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  261
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaacc attctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  262
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  262

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn His Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  263
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  263
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgga acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  264
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  264

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Glu His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  265
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  265
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctacc cgtcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgc agacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaacgt      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaacagct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  266
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  266

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Thr Arg Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Gln Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Arg Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Thr Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  267
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  267
atgggtagca cgggaccggg aattggacca gacgaagctt tacagcgtct taaggaagga       60

aatgctagat ttgtagccga aacacctcgt ccgcagaact tatctgcaaa gcgcctggcg      120

acgtctcaac atggacaaac gccgtatgcc acgatccttt cttgtagtga ttcaagagcg      180

ccagtagagc tgatcttcga cgagggcgta ggcgatctat tcgttatccg tgttgcgggc      240

aatgtagcgg cgacagatga agtgggaaca gcggagtacg gagccgacca ccttaacgtt      300

cctctgcttg tagtgatggg ccatacacaa tgcggtgctg taactgcagt ggtacaagga      360

gcggaggtac atggatcaat cccgatgtta gtggcgccga ttgtgcctgc agtaatggct      420

gtagagaaga gcaacccgaa acacgaccgc gcggctttag tgccgaaggt gatcgaggcg      480

aacgtgtggc aagcgatcga tgacacaatg cgacaatctc caatcatcag agcacgagta      540

gcagcaggca agttaaaagt ggtgggcgct atctaccata tcgacgacgg caaagtagaa      600

tggctgggcg aacatccaat gcaggctcga ttactgaact acacatctgg acctgctaaa      660

gctcaccgct aa                                                          672


<210>  268
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of carbonic anhydrase from D. vulgaris

<400>  268

Met Gly Ser Thr Gly Pro Gly Ile Gly Pro Asp Glu Ala Leu Gln Arg 
1               5                   10                  15      


Leu Lys Glu Gly Asn Ala Arg Phe Val Ala Glu Thr Pro Arg Pro Gln 
            20                  25                  30          


Asn Leu Ser Ala Lys Arg Leu Ala Thr Ser Gln His Gly Gln Thr Pro 
        35                  40                  45              


Tyr Ala Thr Ile Leu Ser Cys Ser Asp Ser Arg Ala Pro Val Glu Leu 
    50                  55                  60                  


Ile Phe Asp Glu Gly Val Gly Asp Leu Phe Val Ile Arg Val Ala Gly 
65                  70                  75                  80  


Asn Val Ala Ala Thr Asp Glu Val Gly Thr Ala Glu Tyr Gly Ala Asp 
                85                  90                  95      


His Leu Asn Val Pro Leu Leu Val Val Met Gly His Thr Gln Cys Gly 
            100                 105                 110         


Ala Val Thr Ala Val Val Gln Gly Ala Glu Val His Gly Ser Ile Pro 
        115                 120                 125             


Met Leu Val Ala Pro Ile Val Pro Ala Val Met Ala Val Glu Lys Ser 
    130                 135                 140                 


Asn Pro Lys His Asp Arg Ala Ala Leu Val Pro Lys Val Ile Glu Ala 
145                 150                 155                 160 


Asn Val Trp Gln Ala Ile Asp Asp Thr Met Arg Gln Ser Pro Ile Ile 
                165                 170                 175     


Arg Ala Arg Val Ala Ala Gly Lys Leu Lys Val Val Gly Ala Ile Tyr 
            180                 185                 190         


His Ile Asp Asp Gly Lys Val Glu Trp Leu Gly Glu His Pro Met Gln 
        195                 200                 205             


Ala Arg Leu Leu Asn Tyr Thr Ser Gly Pro Ala Lys Ala His Arg 
    210                 215                 220             


<210>  269
<211>  714
<212>  DNA
<213>  Desulfovibrio sp. FW1012B

<400>  269
atgaaacggt ttcttgcggc cacggccacc atggcgtttt tgctggcgat gtgcacagcg       60

gtcctggcct cttccggcgg ttcggaagtg agcgccgacg aggccctgtc ccggctcaag      120

gaaggcaaca cgcggttcgt gtcccaggcc aacgtcgcgc cgcatcagga cgccgcccgt      180

cggcatgaaa ccgccaccgg cggacagcac ccgtttgcca cggtcctgtc ctgcgccgat      240

tcccgggccc cggtcgaggt cctcttcgac cagggcgtgg gcgacctttt cgtggtccgc      300

gtggccggca acgtggccgc caccgacgag atcggcacca tcgagtacgg ggccgagcac      360

ctcggcgtgc cgttggtggt ggttctggcc cacaccaaat gcggggcggt cacggcggtg      420

gtcaaaaacg agcccgtcac cgagaacatc ggcaagctgg tggcccccat cgtgccggcg      480

gtcaagggca tcaaggcccg tttcgccgct tcggacgtca acgagatcat cagccggtcc      540

atcgaggcca acatgtggca ggccatcagc gacatctacg ccaaaagccc gatgctcaaa      600

aagatggcgg ccgacggcaa gatcaaggtc gtgggcgccc tctacgacat cgactccggc      660

gaagtgcgct ggttcggcga gcacccgtcc gaaggctccc tgctcgacaa ttaa            714


<210>  270
<211>  237
<212>  PRT
<213>  Desulfovibrio sp. FW1012B

<400>  270

Met Lys Arg Phe Leu Ala Ala Thr Ala Thr Met Ala Phe Leu Leu Ala 
1               5                   10                  15      


Met Cys Thr Ala Val Leu Ala Ser Ser Gly Gly Ser Glu Val Ser Ala 
            20                  25                  30          


Asp Glu Ala Leu Ser Arg Leu Lys Glu Gly Asn Thr Arg Phe Val Ser 
        35                  40                  45              


Gln Ala Asn Val Ala Pro His Gln Asp Ala Ala Arg Arg His Glu Thr 
    50                  55                  60                  


Ala Thr Gly Gly Gln His Pro Phe Ala Thr Val Leu Ser Cys Ala Asp 
65                  70                  75                  80  


Ser Arg Ala Pro Val Glu Val Leu Phe Asp Gln Gly Val Gly Asp Leu 
                85                  90                  95      


Phe Val Val Arg Val Ala Gly Asn Val Ala Ala Thr Asp Glu Ile Gly 
            100                 105                 110         


Thr Ile Glu Tyr Gly Ala Glu His Leu Gly Val Pro Leu Val Val Val 
        115                 120                 125             


Leu Ala His Thr Lys Cys Gly Ala Val Thr Ala Val Val Lys Asn Glu 
    130                 135                 140                 


Pro Val Thr Glu Asn Ile Gly Lys Leu Val Ala Pro Ile Val Pro Ala 
145                 150                 155                 160 


Val Lys Gly Ile Lys Ala Arg Phe Ala Ala Ser Asp Val Asn Glu Ile 
                165                 170                 175     


Ile Ser Arg Ser Ile Glu Ala Asn Met Trp Gln Ala Ile Ser Asp Ile 
            180                 185                 190         


Tyr Ala Lys Ser Pro Met Leu Lys Lys Met Ala Ala Asp Gly Lys Ile 
        195                 200                 205             


Lys Val Val Gly Ala Leu Tyr Asp Ile Asp Ser Gly Glu Val Arg Trp 
    210                 215                 220                 


Phe Gly Glu His Pro Ser Glu Gly Ser Leu Leu Asp Asn 
225                 230                 235         


<210>  271
<211>  978
<212>  DNA
<213>  Desulfomicrobium baculatum (strain DSM 4028)

<400>  271
atgttcatca aacgtcttgt cctggtcttt ctgcttgtct ttgctccgct ttcggtcatg       60

gcgtcatctc ctggcgctca tcccgtaagt cccgaaaatg gattgaagat gctcaccgaa      120

ggaaacctgc ggttcgccct gggccaatcg acccacccca acaccagttt ttccaggcgt      180

ctgctaacca ccacggaagg acaggccccc ttcgctacgg tcatcgcctg ctccgattcg      240

agagtcccgg ttgaaatcct tttcgatcaa gggattggag acttgtttgt gatcaaggtc      300

gccggcaacg tggccgacac ggacgaaatc gggtccgccg aatatggcgt ggaccatctc      360

ggcacgccgg tgctcatggt gctcggacac tcctactgcg gcgccgtcac ggccgtgacc      420

acgggggccg aggtccatgg cagcattccc gccctggtcg acaacatcgt gcctgcggtg      480

gataaggctc gccatgatca cccggatgcc gaaactcccg aactcatcgt ccaggccatc      540

gagaccaatg tctggcaggc catcgaagac ctgctcgaca ccagccacgc tatcgccgac      600

cgggccaagg acggccgggt tatcgtgatt ggagccgtgt acgacatcct caccggcaag      660

gtgaacattc tgggcgctca tcccaaccag accgagctcc tgggcggagt gacgcccccg      720

gtccacgccg aacccgccat gcacgcagaa ccggcaaagg atgcccacgc tccggccgag      780

caggcgccag ccgaacaggc ggacgccgcc catgcggacc cgaccgtgga aaaggccgcg      840

gccgaagggc atgccgaagc ggcggaagcc ccttcctccg gagggttcgg ctttttctcc      900

ttcatcgtgt tcgttcttct gctcattgga gcggtcttcg tgctcgacaa gaagatactc      960

aatcccgacc aggactaa                                                    978


<210>  272
<211>  325
<212>  PRT
<213>  Desulfomicrobium baculatum (strain DSM 4028)

<400>  272

Met Phe Ile Lys Arg Leu Val Leu Val Phe Leu Leu Val Phe Ala Pro 
1               5                   10                  15      


Leu Ser Val Met Ala Ser Ser Pro Gly Ala His Pro Val Ser Pro Glu 
            20                  25                  30          


Asn Gly Leu Lys Met Leu Thr Glu Gly Asn Leu Arg Phe Ala Leu Gly 
        35                  40                  45              


Gln Ser Thr His Pro Asn Thr Ser Phe Ser Arg Arg Leu Leu Thr Thr 
    50                  55                  60                  


Thr Glu Gly Gln Ala Pro Phe Ala Thr Val Ile Ala Cys Ser Asp Ser 
65                  70                  75                  80  


Arg Val Pro Val Glu Ile Leu Phe Asp Gln Gly Ile Gly Asp Leu Phe 
                85                  90                  95      


Val Ile Lys Val Ala Gly Asn Val Ala Asp Thr Asp Glu Ile Gly Ser 
            100                 105                 110         


Ala Glu Tyr Gly Val Asp His Leu Gly Thr Pro Val Leu Met Val Leu 
        115                 120                 125             


Gly His Ser Tyr Cys Gly Ala Val Thr Ala Val Thr Thr Gly Ala Glu 
    130                 135                 140                 


Val His Gly Ser Ile Pro Ala Leu Val Asp Asn Ile Val Pro Ala Val 
145                 150                 155                 160 


Asp Lys Ala Arg His Asp His Pro Asp Ala Glu Thr Pro Glu Leu Ile 
                165                 170                 175     


Val Gln Ala Ile Glu Thr Asn Val Trp Gln Ala Ile Glu Asp Leu Leu 
            180                 185                 190         


Asp Thr Ser His Ala Ile Ala Asp Arg Ala Lys Asp Gly Arg Val Ile 
        195                 200                 205             


Val Ile Gly Ala Val Tyr Asp Ile Leu Thr Gly Lys Val Asn Ile Leu 
    210                 215                 220                 


Gly Ala His Pro Asn Gln Thr Glu Leu Leu Gly Gly Val Thr Pro Pro 
225                 230                 235                 240 


Val His Ala Glu Pro Ala Met His Ala Glu Pro Ala Lys Asp Ala His 
                245                 250                 255     


Ala Pro Ala Glu Gln Ala Pro Ala Glu Gln Ala Asp Ala Ala His Ala 
            260                 265                 270         


Asp Pro Thr Val Glu Lys Ala Ala Ala Glu Gly His Ala Glu Ala Ala 
        275                 280                 285             


Glu Ala Pro Ser Ser Gly Gly Phe Gly Phe Phe Ser Phe Ile Val Phe 
    290                 295                 300                 


Val Leu Leu Leu Ile Gly Ala Val Phe Val Leu Asp Lys Lys Ile Leu 
305                 310                 315                 320 


Asn Pro Asp Gln Asp 
                325 


<210>  273
<211>  786
<212>  DNA
<213>  Desulfovibrio aespoeensis

<400>  273
atggaccgta aattccattc cctggccatc atctgcgcga acgttgtgtt tgccactgtc       60

ctggtggcct tggcccaggc cgagctggtg gtgggcaaag cctcacccga cgaggtcatg      120

gccatgctca aggaaggcaa cgagcggttc gtcgccggga aggcgctcca tgaaaacacc      180

gacgcggagc ggctggccca ggccgggaac gagagccagg ccgatcatgc ctttgccacc      240

gtgctttcct gcgcggattc ccgcgtgccg gtggagcgga ttttcgacgc cggattcata      300

gacctgttcg tggtccgggt ggcgggcaat gtggtgaaaa ccgacgaggc cgggtccatc      360

gagtacgggc tggcccatgt gcacacgccg gtgctggtgg tcatggggca cacccaatgc      420

ggcgctgtcg cagccgtgac cgacgtggtg caggggcgca agctgacgtt tgaacgcaat      480

attccgccgc tggtcgctcc catcatcccg gctgtgcagc gggccatcgc cgcccatccc      540

gatgcttcgg gcagggccgt gttgcccttt gccatcgagg agaacgtctg gcagggcatc      600

acggacctgt tcatgcagag cccggccacc cgcatgatcg tcaggtccgg caaggtcaag      660

gtggtcgggg cgatttacga cgtcgggact ggccgcgtca gttggctgcc cgagtccagg      720

gttctcgaac tgctggacgc ggtggagcgc gaccctggcc gtgccatgaa cgccatggcc      780

gagtag                                                                 786


<210>  274
<211>  261
<212>  PRT
<213>  Desulfovibrio aespoeensis

<400>  274

Met Asp Arg Lys Phe His Ser Leu Ala Ile Ile Cys Ala Asn Val Val 
1               5                   10                  15      


Phe Ala Thr Val Leu Val Ala Leu Ala Gln Ala Glu Leu Val Val Gly 
            20                  25                  30          


Lys Ala Ser Pro Asp Glu Val Met Ala Met Leu Lys Glu Gly Asn Glu 
        35                  40                  45              


Arg Phe Val Ala Gly Lys Ala Leu His Glu Asn Thr Asp Ala Glu Arg 
    50                  55                  60                  


Leu Ala Gln Ala Gly Asn Glu Ser Gln Ala Asp His Ala Phe Ala Thr 
65                  70                  75                  80  


Val Leu Ser Cys Ala Asp Ser Arg Val Pro Val Glu Arg Ile Phe Asp 
                85                  90                  95      


Ala Gly Phe Ile Asp Leu Phe Val Val Arg Val Ala Gly Asn Val Val 
            100                 105                 110         


Lys Thr Asp Glu Ala Gly Ser Ile Glu Tyr Gly Leu Ala His Val His 
        115                 120                 125             


Thr Pro Val Leu Val Val Met Gly His Thr Gln Cys Gly Ala Val Ala 
    130                 135                 140                 


Ala Val Thr Asp Val Val Gln Gly Arg Lys Leu Thr Phe Glu Arg Asn 
145                 150                 155                 160 


Ile Pro Pro Leu Val Ala Pro Ile Ile Pro Ala Val Gln Arg Ala Ile 
                165                 170                 175     


Ala Ala His Pro Asp Ala Ser Gly Arg Ala Val Leu Pro Phe Ala Ile 
            180                 185                 190         


Glu Glu Asn Val Trp Gln Gly Ile Thr Asp Leu Phe Met Gln Ser Pro 
        195                 200                 205             


Ala Thr Arg Met Ile Val Arg Ser Gly Lys Val Lys Val Val Gly Ala 
    210                 215                 220                 


Ile Tyr Asp Val Gly Thr Gly Arg Val Ser Trp Leu Pro Glu Ser Arg 
225                 230                 235                 240 


Val Leu Glu Leu Leu Asp Ala Val Glu Arg Asp Pro Gly Arg Ala Met 
                245                 250                 255     


Asn Ala Met Ala Glu 
            260     


<210>  275
<211>  1200
<212>  DNA
<213>  Desulfovibrio desulfuricans (strain G20)

<400>  275
atgattttca aatcgcggaa aaccctgctg gctgcccttg gactggtggc tttgtttctt       60

gttttcggtg cgttttcgtg cagcagtgac agcacggtgg aacgtacgcc cgaagctgcg      120

ctgaaagccc tcagacaggg caacgaacgt tttgcagccg gcgaagctgt gcacccccgg      180

actgatgccg cgcgtctggc gcaggccggc acggaaagtc agggcgatca tgcatacgcc      240

acagtgctgg cctgttctga ctcacgcgtg cccgtggaac gcctgtttga tgccggagtc      300

atggatattt ttgtggtgcg tgtggccggc aacgtagtgc agggcgacga agccggttcc      360

attgaatatg gtctggccca tgtgaaaacc cctgtgctgg tggtgctggg acatacccag      420

tgcggtgccg tgaccgccgt gaccgcagcg ctggaaggac acggtcatgc gctggaacgc      480

aatatccccg gtctggtcaa gcccgtcatt cctgccgtgc agcaggccat gcaggaacac      540

cccgatgtgc acggtgcgga tctggtgccc ttcggcattg aaaataatgt ctggcagaac      600

atccgtaatc ttttcatgct gagccccgcc accagagatc tggtaaaatc cggcaaggtg      660

gctgttgtcg gagccgtata cgatgtgtcc accggcaagg ttgaatggct gcccgccgcc      720

cgcgtggatg aaattcttgc cgcagtggaa gcagaccccg cccgtgcgac ggaagccatg      780

gcttccgggg ccggcgccgc acagccggac catgacggcg cgcccgctgc tgcagcagaa      840

aatgaacagg ccgtatccga agctgaacac gctgctgatg ctgcagaaga cgccgccgga      900

tcttccggtt ccggcgcaga ggttgaaacc gttgaagccg ttgaggttcc cgcggccaac      960

gccacagacg caggcgaaga gaccgtgacg caggaagccg cagtgccctg ccccggtcag     1020

atggcccgcg acaccatggc cattcatgca caggctgcgc tggaacaggc tgaaatggcg     1080

ggcaaggccg cacaggttgc cgccgaagct gccgcaaagg ctgccgaggc cgcgggagaa     1140

gccgcccgtg ccgccgaagc cgctgcactg gcggcgcagg ccgctcaggc gcagcagtag     1200


<210>  276
<211>  399
<212>  PRT
<213>  Desulfovibrio desulfuricans (strain G20)

<400>  276

Met Ile Phe Lys Ser Arg Lys Thr Leu Leu Ala Ala Leu Gly Leu Val 
1               5                   10                  15      


Ala Leu Phe Leu Val Phe Gly Ala Phe Ser Cys Ser Ser Asp Ser Thr 
            20                  25                  30          


Val Glu Arg Thr Pro Glu Ala Ala Leu Lys Ala Leu Arg Gln Gly Asn 
        35                  40                  45              


Glu Arg Phe Ala Ala Gly Glu Ala Val His Pro Arg Thr Asp Ala Ala 
    50                  55                  60                  


Arg Leu Ala Gln Ala Gly Thr Glu Ser Gln Gly Asp His Ala Tyr Ala 
65                  70                  75                  80  


Thr Val Leu Ala Cys Ser Asp Ser Arg Val Pro Val Glu Arg Leu Phe 
                85                  90                  95      


Asp Ala Gly Val Met Asp Ile Phe Val Val Arg Val Ala Gly Asn Val 
            100                 105                 110         


Val Gln Gly Asp Glu Ala Gly Ser Ile Glu Tyr Gly Leu Ala His Val 
        115                 120                 125             


Lys Thr Pro Val Leu Val Val Leu Gly His Thr Gln Cys Gly Ala Val 
    130                 135                 140                 


Thr Ala Val Thr Ala Ala Leu Glu Gly His Gly His Ala Leu Glu Arg 
145                 150                 155                 160 


Asn Ile Pro Gly Leu Val Lys Pro Val Ile Pro Ala Val Gln Gln Ala 
                165                 170                 175     


Met Gln Glu His Pro Asp Val His Gly Ala Asp Leu Val Pro Phe Gly 
            180                 185                 190         


Ile Glu Asn Asn Val Trp Gln Asn Ile Arg Asn Leu Phe Met Leu Ser 
        195                 200                 205             


Pro Ala Thr Arg Asp Leu Val Lys Ser Gly Lys Val Ala Val Val Gly 
    210                 215                 220                 


Ala Val Tyr Asp Val Ser Thr Gly Lys Val Glu Trp Leu Pro Ala Ala 
225                 230                 235                 240 


Arg Val Asp Glu Ile Leu Ala Ala Val Glu Ala Asp Pro Ala Arg Ala 
                245                 250                 255     


Thr Glu Ala Met Ala Ser Gly Ala Gly Ala Ala Gln Pro Asp His Asp 
            260                 265                 270         


Gly Ala Pro Ala Ala Ala Ala Glu Asn Glu Gln Ala Val Ser Glu Ala 
        275                 280                 285             


Glu His Ala Ala Asp Ala Ala Glu Asp Ala Ala Gly Ser Ser Gly Ser 
    290                 295                 300                 


Gly Ala Glu Val Glu Thr Val Glu Ala Val Glu Val Pro Ala Ala Asn 
305                 310                 315                 320 


Ala Thr Asp Ala Gly Glu Glu Thr Val Thr Gln Glu Ala Ala Val Pro 
                325                 330                 335     


Cys Pro Gly Gln Met Ala Arg Asp Thr Met Ala Ile His Ala Gln Ala 
            340                 345                 350         


Ala Leu Glu Gln Ala Glu Met Ala Gly Lys Ala Ala Gln Val Ala Ala 
        355                 360                 365             


Glu Ala Ala Ala Lys Ala Ala Glu Ala Ala Gly Glu Ala Ala Arg Ala 
    370                 375                 380                 


Ala Glu Ala Ala Ala Leu Ala Ala Gln Ala Ala Gln Ala Gln Gln 
385                 390                 395                 


<210>  277
<211>  714
<212>  DNA
<213>  Desulfovibrio magneticus (strain ATCC 700980)

<400>  277
atgaaacggt tcgttgcggc gtttgcggga gcggtcatca cgttttccat ggctggggcg       60

gccatggcgt tttccggcgg ggcgggcatt tccgccgatg aggcgctggc ccgcctcaag      120

gaaggcaaca cccgctatgt ggccggcgcg gccgtgaccc cgcgtcagga cgcggcccgg      180

cggcatgaaa ccgccaccgg cggccagcat ccctttgtct cggtgctgtc ctgcgccgat      240

tcccgcgtgc ccgtggaaac cgtgttcgac cagggcattg gcgatgtgtt cgtgatccgc      300

gtggccggca acgtggccaa caccgacgag atcggtacca tcgaatacgg gaccgaacac      360

ctgggcaccc ccctggtggt ggtcctggcc cacaccaaat gcggcgcggt cacggccgtg      420

gtcaagggcg agcatgtcac cgaaaacatc ggcaagctcg tggcccccat cgtgccggcc      480

gtggcttcgg tgaaaagccg ctttgcctcg ggcgacttga acgagcttat caaccgctcc      540

atcgaggcca acatgtggca ggccatcgcc gacatgtacg ccaaaagccc cttgctcaag      600

aagatggcgg ccgacggcaa gatcaaggtt gtgggcgcgc tctatgacat cgattccggc      660

gatgtccatt ggtttggcga gcatccgtcc aacgccaacc ttatcggcaa gtag            714


<210>  278
<211>  237
<212>  PRT
<213>  Desulfovibrio magneticus (strain ATCC 700980)

<400>  278

Met Lys Arg Phe Val Ala Ala Phe Ala Gly Ala Val Ile Thr Phe Ser 
1               5                   10                  15      


Met Ala Gly Ala Ala Met Ala Phe Ser Gly Gly Ala Gly Ile Ser Ala 
            20                  25                  30          


Asp Glu Ala Leu Ala Arg Leu Lys Glu Gly Asn Thr Arg Tyr Val Ala 
        35                  40                  45              


Gly Ala Ala Val Thr Pro Arg Gln Asp Ala Ala Arg Arg His Glu Thr 
    50                  55                  60                  


Ala Thr Gly Gly Gln His Pro Phe Val Ser Val Leu Ser Cys Ala Asp 
65                  70                  75                  80  


Ser Arg Val Pro Val Glu Thr Val Phe Asp Gln Gly Ile Gly Asp Val 
                85                  90                  95      


Phe Val Ile Arg Val Ala Gly Asn Val Ala Asn Thr Asp Glu Ile Gly 
            100                 105                 110         


Thr Ile Glu Tyr Gly Thr Glu His Leu Gly Thr Pro Leu Val Val Val 
        115                 120                 125             


Leu Ala His Thr Lys Cys Gly Ala Val Thr Ala Val Val Lys Gly Glu 
    130                 135                 140                 


His Val Thr Glu Asn Ile Gly Lys Leu Val Ala Pro Ile Val Pro Ala 
145                 150                 155                 160 


Val Ala Ser Val Lys Ser Arg Phe Ala Ser Gly Asp Leu Asn Glu Leu 
                165                 170                 175     


Ile Asn Arg Ser Ile Glu Ala Asn Met Trp Gln Ala Ile Ala Asp Met 
            180                 185                 190         


Tyr Ala Lys Ser Pro Leu Leu Lys Lys Met Ala Ala Asp Gly Lys Ile 
        195                 200                 205             


Lys Val Val Gly Ala Leu Tyr Asp Ile Asp Ser Gly Asp Val His Trp 
    210                 215                 220                 


Phe Gly Glu His Pro Ser Asn Ala Asn Leu Ile Gly Lys 
225                 230                 235         


