                         SEQUENCE LISTING

<110>  Ablynx NV
 
<120>  PHARMACEUTICAL COMPOSITIONS FOR CUTANEOUS ADMINISTRATION

<130>  P10-012-PCT-1

<150>  US 61/358,588
<151>  2010-06-25

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody construct

<400>  1

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ile Phe Ser Leu Pro 
            20                  25                  30          


Ala Ser Gly Asn Ile Phe Asn Leu Leu Thr Ile Ala Trp Tyr Arg Gln 
        35                  40                  45              


Ala Pro Gly Lys Gly Arg Glu Leu Val Ala Thr Ile Asn Ser Gly Ser 
    50                  55                  60                  


Arg Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg 
65                  70                  75                  80  


Asp Asn Ser Lys Lys Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro 
                85                  90                  95      


Glu Asp Thr Ala Val Tyr Tyr Cys Gln Thr Ser Gly Ser Gly Ser Pro 
            100                 105                 110         


Asn Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
    130                 135                 140                 


Leu Val Gln Pro Gly Asn Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
145                 150                 155                 160 


Phe Thr Phe Ser Ser Phe Gly Met Ser Trp Val Arg Gln Ala Pro Gly 
                165                 170                 175     


Lys Gly Leu Glu Trp Val Ser Ser Ile Ser Gly Ser Gly Ser Asp Thr 
            180                 185                 190         


Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
        195                 200                 205             


Ala Lys Thr Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp 
    210                 215                 220                 


Thr Ala Val Tyr Tyr Cys Thr Ile Gly Gly Ser Leu Ser Arg Ser Ser 
225                 230                 235                 240 


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro 
            260                 265                 270         


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser 
        275                 280                 285             


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu 
    290                 295                 300                 


Phe Val Ala Arg Ile Ser Gln Gly Gly Thr Ala Ile Tyr Tyr Ala Asp 
305                 310                 315                 320 


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
                325                 330                 335     


Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr 
            340                 345                 350         


Tyr Cys Ala Lys Asp Pro Ser Pro Tyr Tyr Arg Gly Ser Ala Tyr Leu 
        355                 360                 365             


Leu Ser Gly Ser Tyr Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val 
    370                 375                 380                 


Ser Ser 
385     


<210>  2
<211>  386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody construct

<400>  2

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ile Phe Ser Leu Pro 
            20                  25                  30          


Ala Ser Gly Asn Ile Phe Asn Leu Leu Thr Ile Ala Trp Tyr Arg Gln 
        35                  40                  45              


Ala Pro Gly Lys Gly Arg Glu Leu Val Ala Thr Ile Asn Ser Gly Ser 
    50                  55                  60                  


Arg Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg 
65                  70                  75                  80  


Asp Asn Ser Lys Lys Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Pro 
                85                  90                  95      


Glu Asp Thr Ala Val Tyr Tyr Cys Gln Thr Ser Gly Ser Gly Ser Pro 
            100                 105                 110         


Asn Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
    130                 135                 140                 


Leu Val Gln Pro Gly Asn Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
145                 150                 155                 160 


Phe Thr Phe Ser Ser Phe Gly Met Ser Trp Val Arg Gln Ala Pro Gly 
                165                 170                 175     


Lys Gly Leu Glu Trp Val Ser Ser Ile Ser Gly Ser Gly Ser Asp Thr 
            180                 185                 190         


Leu Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
        195                 200                 205             


Ala Lys Thr Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp 
    210                 215                 220                 


Thr Ala Val Tyr Tyr Cys Thr Ile Gly Gly Ser Leu Ser Arg Ser Ser 
225                 230                 235                 240 


Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Ser Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro 
            260                 265                 270         


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser 
        275                 280                 285             


Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu 
    290                 295                 300                 


Phe Val Ala Arg Ile Ser Gln Gly Gly Thr Ala Ile Tyr Tyr Ala Asp 
305                 310                 315                 320 


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr 
                325                 330                 335     


Leu Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr 
            340                 345                 350         


Tyr Cys Ala Lys Asp Pro Ser Pro Tyr Tyr Arg Gly Ser Ala Tyr Leu 
        355                 360                 365             


Leu Ser Gly Ser Tyr Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val 
    370                 375                 380                 


Ser Ser 
385     


<210>  3
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody construct

<400>  3

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ile Phe Ser Leu Pro 
            20                  25                  30          


Ala Ser Gly Asn Ile Phe Asn Leu Leu Thr Ile Ala Trp Tyr Arg Gln 
        35                  40                  45              


Ala Pro Gly Lys Gly Arg Glu Leu Val Ala Thr Ile Asn Ser Gly Ser 
    50                  55                  60                  


Arg Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg 
65                  70                  75                  80  


Asp Asn Ser Lys Lys Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Pro 
                85                  90                  95      


Glu Asp Thr Ala Val Tyr Tyr Cys Gln Thr Ser Gly Ser Gly Ser Pro 
            100                 105                 110         


Asn Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
                165                 170                 175     


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Leu Ser Ser Tyr 
            180                 185                 190         


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Phe Val 
        195                 200                 205             


Ala Arg Ile Ser Gln Gly Gly Thr Ala Ile Tyr Tyr Ala Asp Ser Val 
    210                 215                 220                 


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
225                 230                 235                 240 


Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                245                 250                 255     


Ala Lys Asp Pro Ser Pro Tyr Tyr Arg Gly Ser Ala Tyr Leu Leu Ser 
            260                 265                 270         


Gly Ser Tyr Asp Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        275                 280                 285             


<210>  4
<211>  1158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nanobody construct

<400>  4
gaggtgcaat tgttagagtc cggtggtggt ttagttcaac ctggtggttc cttgagattg       60

tcttgtgctg cctccggtag aattttctct ctgcccgcct ccggtaacat tttcaacctg      120

ctgactatcg cttggtatag acaggctcca ggaaagggta gagagttggt tgctaccatc      180

aactccggtt ccagaactta ctacgccgac tccgttaaag gaagattcac catctccaga      240

gacaactcca agaaaaccct gtacctgcag atgaactctc ttagaccaga ggacaccgct      300

gtttactact gtcaaacctc tggttctggt tctccaaact tctggggtca aggtactctg      360

gttactgttt cctctggtgg tggtggttct ggtggtggat ctgaggttca gttggttgaa      420

tccggtggag gattggttca acccggtaac tctttgagac tttcctgtgc cgcttctggt      480

tttactttct cctccttcgg aatgtcttgg gttagacaag ccccaggtaa aggattggag      540

tgggtttcct ctatttctgg ttccggttcc gatactttgt acgctgatag tgtcaaggga      600

agattcacta tcagtagaga caacgctaag accactctgt acttgcaaat gaattcatta      660

agacccgagg acactgccgt ctattattgt actatcggtg gttctttgtc cagatcttcc      720

cagggaactt tggttacagt tagttccggt ggtggaggta gtggtggagg ttccgaagtt      780

cagttgttgg aaagtggtgg tggacttgtt caaccaggtg gaagtttgag attgagttgc      840

gccgcttccg gtagaacttt gtcctcttac gccatgggtt ggtttagaca ggcacccggt      900

aaaggtagag aattcgtggc cagaatctcc caaggtggta ctgctatcta ctacgctgat      960

tcagtgaaag gaagattcac aatttctaga gataacagta agaacacact ttaccttcaa     1020

atgaacagtc ttagacccga agatacagcc gtgtactact gtgctaagga cccatctcca     1080

tactacagag gttccgccta cttgttgtct ggttcttacg actcttgggg acagggaact     1140

ctggtcaccg tctcctca                                                   1158


<210>  5
<211>  1158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nanobody construct

<400>  5
gaggtgcaat tgctggagtc tgggggagga ttggtacaac caggaggttc tttgagactg       60

tcttgtgcag cttctggtcg tatattcagt ttgcctgcta gtgggaatat ttttaacctg      120

ctcactatcg cttggtatcg tcaagctcca ggtaaaggta gagagcttgt tgccacgatt      180

aactctggtt ccaggacata ttacgctgat agcgttaagg gcagattcac aatctcgcga      240

gataattcga aaaaaactgt ttacctgcaa atgaacagcc ttagaccaga agatactgcc      300

gtctattact gtcaaacttc agggtctggt agtcccaact tttggggtca agggaccctg      360

gtcacggtct cctcaggagg tggcggatcc ggcggaggta gtgaggtgca gctggtggag      420

tctgggggtg gattggttca acctggaaat agcctgagac tatcttgtgc agcttcaggt      480

ttcacctttt cctcattcgg tatgagttgg gttagacaag ctccaggaaa gggtttggaa      540

tgggtctcgt caatttctgg tagcggatct gatacgttgt acgctgactc agtaaaggga      600

aggttcacta tttcgcgtga caatgccaaa accacgttgt acttgcagat gaattcactc      660

agacctgaag ataccgctgt gtactattgc acgataggtg gatcacttag tcggtctagt      720

caagggaccc tggtcacggt ctcctcagga ggtggcgggt ccggaggagg tagtgaggtg      780

cagctgctgg agtctggggg aggtttggta caaccaggtg gatctctgag attgtcttgt      840

gctgcttctg gaagaacact ttcctcgtat gctatgggat ggtttagaca ggcaccaggt      900

aaaggcaggg aatttgtagc acgaatcagt caaggaggaa ccgcaattta ttacgcagac      960

tctgtaaagg gtcgttttac aatatcccga gataacagta agaataccct ctacctccaa     1020

atgaactccc taagaccaga agacacagcc gtttattatt gcgctaaaga tccatcaccc     1080

tactatcgag gatctgcgta tcttttgtct gggtcctacg attcttgggg acaagggacc     1140

ctggtcaccg tctcctca                                                   1158


<210>  6
<211>  864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nanobody construct

<400>  6
gaggtgcaat tgctggagtc tgggggagga ttggtacaac caggaggttc tttgagactg       60

tcttgtgcag cttctggtcg tatattcagt ttgcctgcta gtgggaatat ttttaacctg      120

ctcactatcg cttggtatcg tcaagctcca ggtaaaggta gagagcttgt tgccacgatt      180

aactctggtt ccaggacata ttacgctgat agcgttaagg gcagattcac aatctcgcga      240

gataattcga aaaaaactgt ttacctgcaa atgaacagcc ttagaccaga agatactgcc      300

gtctattact gtcaaacttc agggtctggt agtcccaact tttggggtca agggaccctg      360

gtcacggtct cctcaggagg tggcggatcc ggcggaggag gtagtggtgg tggaggttct      420

ggtggtggag gatcaggtgg aggtggaagt ggaggtggtg gttcaggagg tggtggttca      480

gaggtgcagc tgctggagtc tgggggaggt ttggtacaac caggtggatc tctgagattg      540

tcttgtgctg cttctggaag aacactttcc tcgtatgcta tgggatggtt tagacaggca      600

ccaggtaaag gcagggaatt tgtagcacga atcagtcaag gaggaaccgc aatttattac      660

gcagactctg taaagggtcg ttttacaata tcccgagata acagtaagaa taccctctac      720

ctccaaatga actccctaag accagaagac acagccgttt attattgcgc taaagatcca      780

tcaccctact atcgaggatc tgcgtatctt ttgtctgggt cctacgattc ttggggacaa      840

gggaccctgg tcaccgtctc ctca                                             864


