                         SEQUENCE LISTING

<110>  Azriel, Julia
       Kaur, Zach
       Meyers, Jonathan
       Parody, Todd
       Zhao, Yan
       DiMarchi, Richard
 
<120>  SINGLE CHAIN INSULIN AGONISTS EXHIBITING HIGH ACTIVITY AT THE 
       INSULIN RECEPTOR

<130>  32993-210602

<150>  61/355,366
<151>  2010-06-16

<150>  61/433,500
<151>  2011-01-17

<160>  197   

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  1

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  2
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  2

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  3
<211>  70
<212>  PRT
<213>  Homo sapiens

<400>  3

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Gln Phe 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr Gly Tyr Gly 
            20                  25                  30          


Ser Ser Ser Arg Arg Ala Pro Gln Thr Gly Ile Val Asp Glu Cys Cys 
        35                  40                  45              


Phe Arg Ser Cys Asp Leu Arg Arg Leu Glu Met Tyr Cys Ala Pro Leu 
    50                  55                  60                  


Lys Pro Ala Lys Ser Ala 
65                  70  


<210>  4
<211>  67
<212>  PRT
<213>  Homo sapiens

<400>  4

Ala Tyr Arg Pro Ser Glu Thr Leu Cys Gly Gly Glu Leu Val Asp Thr 
1               5                   10                  15      


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Ser Arg Pro Ala 
            20                  25                  30          


Ser Arg Val Ser Arg Arg Ser Arg Gly Ile Val Glu Glu Cys Cys Phe 
        35                  40                  45              


Arg Ser Cys Asp Leu Ala Leu Leu Glu Thr Tyr Cys Ala Thr Pro Ala 
    50                  55                  60                  


Lys Ser Glu 
65          


<210>  5
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  5

Gly Ile Val Asp Glu Cys Cys Phe Arg Ser Cys Asp Leu Arg Arg Leu 
1               5                   10                  15      


Glu Met Tyr Cys Ala 
            20      


<210>  6
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  6

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Gln Phe 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr 
            20                  25                  


<210>  7
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  7

Gly Ile Val Glu Glu Cys Cys Phe Arg Ser Cys Asp Leu Ala Leu Leu 
1               5                   10                  15      


Glu Thr Tyr Cys Ala 
            20      


<210>  8
<211>  32
<212>  PRT
<213>  Homo sapiens

<400>  8

Ala Tyr Arg Pro Ser Glu Thr Leu Cys Gly Gly Glu Leu Val Asp Thr 
1               5                   10                  15      


Leu Gln Phe Val Cys Gly Asp Arg Gly Phe Tyr Phe Ser Arg Pro Ala 
            20                  25                  30          


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  The Xaa at position 1 represents an amino acid selected from 
       glycine, alanine, valine, leucine, isoleucine, proline, 
       phenylalanine and methionine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 represents any amino acid other than 
       tyrosine

<220>
<221>  MISC_FEATURE
<222>  (3)..(6)
<223>  The Xaas at positions 3-6 are independently any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  The Xaas at positions 7 and 8 are independently selected from 
       arginine, lysine or ornithine

<400>  9

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  The Xaa at position 1 represents an amino acid selected from 
       glycine, alanine, valine, leucine, isoleucine, proline, 
       phenylalanine and methionine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 represents any amino acid other than 
       tyrosine

<220>
<221>  MISC_FEATURE
<222>  (3)..(6)
<223>  The Xaas at positions 3-6 are independently any amino acid

<400>  10

Xaa Xaa Xaa Xaa Xaa Xaa Arg Arg 
1               5               


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  11

Pro Gly Pro Glu 
1               


<210>  12
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  12

Phe Val Asn Gln 
1               


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  The Xaas at positions 1-5 are independently any amino acid

<400>  13

Xaa Xaa Xaa Xaa Xaa 
1               5   


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  14

Ala Tyr Arg Pro Ser Glu 
1               5       


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  The Xaas at positions 1-4 are independently any amino acid

<400>  15

Xaa Xaa Xaa Xaa 
1               


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence

<400>  16

Tyr Thr Pro Lys Thr 
1               5   


<210>  17
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  17

Gly Tyr Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  18
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  18

Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5               


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa at position 4 is aspartic acid or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine, threonine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is serine, arginine, ornithine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is tyrosine, arginine, ornathine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is glutamine, glutamic acid, arginine, 
       ornithine, alanine, lysine or leucine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is methionine, asparagine, glutamine, glutamic
       acid, aspartic acid or threonine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine, serine, valine, 
       threonine, isoleucine, leucine, glutamine, glutamic acid, 
       asparagine, aspartic acid, histidine, tryptophan, tyrosine, or 
       methionine

<400>  19

Gly Ile Val Xaa Glu Cys Cys Xaa Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Xaa Xaa Xaa Cys Xaa 
            20      


<210>  20
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is aspartic acid, glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is alanine or threonine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is tyrosine or 4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 12 is glutamic acid, aspartic acid or asparagine

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is alanine, ornithine, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is tyrosine, phenylalanine or 
       4-amino-phenylalanine

<400>  20

Xaa Leu Cys Gly Xaa Xaa Leu Val Xaa Xaa Leu Xaa Leu Val Cys Gly 
1               5                   10                  15      


Xaa Xaa Gly Phe Xaa 
            20      


<210>  21
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is aspartic acid, glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is alanine or threonine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 12 is glutamic acid, aspartic acid or asparagine

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is alanine, ornithine, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is tyrosine, histidine, asparagine or 4-amino-phenylalanine

<400>  21

Xaa Leu Cys Gly Xaa Xaa Leu Val Xaa Xaa Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Xaa Xaa Gly Phe Xaa 
            20      


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa at position 4 is aspartic acid or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine, threonine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is serine, arginine, ornithine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is isoleucine or serine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is aspartic acid or serine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is tyrosine, arginine, ornathine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is glutamine, glutamic acid, arginine, 
       ornithine, alanine, lysine or leucine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is methionine, asparagine, glutamine, glutamic
       acid, aspartic acid or threonine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine, serine, valine, 
       threonine, isoleucine, leucine, glutamine, glutamic acid, 
       asparagine, aspartic acid, histidine, tryptophan, tyrosine, or 
       methionine

<400>  22

Gly Ile Val Xaa Xaa Cys Cys Xaa Xaa Xaa Cys Xaa Leu Xaa Xaa Leu 
1               5                   10                  15      


Xaa Xaa Xaa Cys Xaa 
            20      


<210>  23
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  The Xaa at position 1 represents an amino acid selected from 
       glycine, alanine, valine, leucine, isoleucine, proline, 
       phenylalanine and methionine

<220>
<221>  MISC_FEATURE
<222>  (3)..(6)
<223>  The Xaas at positions 3-6 are independently any amino acid

<400>  23

Xaa Ala Xaa Xaa Xaa Xaa Arg Arg 
1               5               


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  The Xaas at positions 1-4 are independently any amino acid

<400>  24

Xaa Xaa Xaa Xaa 
1               


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  The Xaas at positions 1-7 are independently any amino acid

<400>  25

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg 
1               5               


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(6)
<223>  The Xaas at positions 1-5 are independently any amino acid

<400>  26

Xaa Xaa Xaa Xaa Xaa Ser Arg Arg 
1               5               


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  The Xaa at positions 1 is glycine, alanine, valine, leucine, 
       isoleucine, proline or methionine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at positions 2 is any non-aromatic amino acid

<400>  27

Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5               


<210>  28
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position1 is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(7)
<223>  Xaas at positions 3-7 are independently any amino acid

<400>  28

Xaa Ala Xaa Xaa Xaa Xaa Xaa Arg 
1               5               


<210>  29
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position1 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine and methionine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is any non-aromatic amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  Xaas at positions 7 and 8 are independently arginine, lysine or 
       ornithine

<400>  29

Xaa Xaa Gly Ser Ser Ser Xaa Xaa 
1               5               


<210>  30
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position1 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine and methionine

<400>  30

Xaa Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is any amino acid

<400>  31

Gly Xaa Gly Ser Ser Ser Arg Arg 
1               5               


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  32

Gly Ala Gly Ser Ser Ser Arg Arg 
1               5               


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  33

Gly Ala Gly Ser Ser Ser Arg Arg Ala 
1               5                   


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  34

Gly Ala Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10  


<210>  35
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  35

Gly Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10      


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  36

Gly Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  37
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  The Xaas at positions 7 and 8 are independently arginine, lysine 
       or ornithine

<400>  37

Gly Tyr Gly Ser Ser Ser Xaa Xaa Ala Pro Gln Thr 
1               5                   10          


<210>  38
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaas at position 2 is alanine, valine, leucine, isoleucine 
       or proline

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  The Xaas at position 8 is arginine, lysine or ornithine

<400>  38

Gly Xaa Gly Ser Ser Ser Arg Xaa Ala Pro Gln Thr 
1               5                   10          


<210>  39
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  39

Pro Tyr Gly Ser Ser Ser Arg Arg 
1               5               


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  40

Pro Ala Gly Ser Ser Ser Arg Arg 
1               5               


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  41

Pro Ala Gly Ser Ser Ser Arg Arg Ala 
1               5                   


<210>  42
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  42

Pro Ala Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10  


<210>  43
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  43

Pro Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10      


<210>  44
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  44

Pro Ala Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  45
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  The Xaa at position 8 is arginine, lysine or ornithine

<400>  45

Gly Tyr Gly Ser Ser Ser Arg Xaa 
1               5               


<210>  46
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position1 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine and methionine

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is any non-aromatic amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  Xaas at positions 7 and 8 are independently arginine, lysine or 
       ornithine

<400>  46

Xaa Xaa Gly Ser Ser Ser Xaa Xaa Ala Pro Gln Thr 
1               5                   10          


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  the Xaa at position 1 is glycine, glutamic acid or aspartic acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(6)
<223>  the Xaas at positions 2-6 are independently glutamic acid or 
       aspartic acid

<400>  47

Xaa Xaa Xaa Xaa Xaa Xaa Lys 
1               5           


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(6)
<223>  the Xaas at positions 2-6 are independently glutamic acid or 
       aspartic acid

<400>  48

Gly Xaa Xaa Xaa Xaa Xaa Lys 
1               5           


<210>  49
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  the Xaas at positions 1-5 are independently glutamic acid or 
       aspartic acid

<400>  49

Xaa Xaa Xaa Xaa Xaa Arg Lys 
1               5           


<210>  50
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin analog


<220>
<221>  MISC_FEATURE
<222>  (28)..(28)
<223>  The Xaa at position 28 is alanine lysine, ornithine or arginine

<400>  50

Gly Glu Glu Glu Glu Glu Lys Gly Pro Glu His Leu Cys Gly Ala His 
1               5                   10                  15      


Leu Val Asp Ala Leu Tyr Leu Val Cys Gly Asp Xaa Gly Phe Tyr 
            20                  25                  30      


<210>  51
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  The Xaa at position 7 is arginine, lysine or ornithine

<400>  51

Gly Tyr Gly Ser Ser Ser Xaa Arg 
1               5               


<210>  52
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is asparagine or glycine

<400>  52

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Xaa Asn Xaa Cys Xaa 
            20      


<210>  53
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  53

Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  54
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is phenylalanine and desamino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is histidine and threonine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<400>  54

Xaa Val Asn Gln Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr 
            20                  25          


<210>  55
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine or 4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine or asparagine

<400>  55

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Xaa 
            20      


<210>  56
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine or phenylalnine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is arginine, lysine, ornithine or alanine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is arginine, lysine, ornithine or alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is arginine, lysine, ornithine or leucine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is methionine, asparagine or threonine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine or asparagine

<400>  56

Gly Ile Val Asp Glu Cys Cys Xaa Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Xaa Xaa Xaa Cys Xaa 
            20      


<210>  57
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is arginine, lysine, ornithine or alanine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is arginine, lysine, ornithine or alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is arginine, lysine, ornithine or leucine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamic acid, aspartic acid, asparagine, 
       lysine, ornithine or glutamine

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is methionine, asparagine or threonine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine or asparagine

<400>  57

Gly Ile Val Asp Glu Cys Cys His Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Xaa Xaa Xaa Cys Xaa 
            20      


<210>  58
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is aspartic acid or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is alanine or threonine

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is alanine, ornithine, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is tyrosine, histidine, asparagine or phenylalanine

<400>  58

Xaa Leu Cys Gly Xaa Xaa Leu Val Xaa Xaa Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Asp Xaa Gly Phe Xaa 
            20      


<210>  59
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is arginine, ornithine, or lysine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is arginine, ornithine, or lysine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is arginine, ornithine, or lysine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine or asparagine

<400>  59

Gly Ile Val Asp Glu Cys Cys His Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Xaa Met Xaa Cys Xaa 
            20      


<210>  60
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is alanine, ornithine, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is tyrosine or phenylalanine

<400>  60

Xaa Leu Cys Gly Ala Xaa Leu Val Asp Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Asp Xaa Gly Phe Xaa 
            20      


<210>  61
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  analog of IGF B chain


<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  The Xaa at position 18 is ornithine, lysine or arginine

<400>  61

His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Asp Xaa Gly Phe Tyr 
            20      


<210>  62
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  analog of IGF B chain


<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is ornithine, lysine or arginine

<400>  62

Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr 
            20                  


<210>  63
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  analog of IGF B chain


<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is ornithine, lysine or arginine

<400>  63

Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Pro Lys Thr 
            20                  25                  


<210>  64
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  analog of IGF B chain


<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is ornithine, lysine or arginine

<400>  64

Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Lys Pro Thr 
            20                  25                  


<210>  65
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is ornithine

<400>  65

Gly Tyr Gly Ser Ser Ser Xaa Arg 
1               5               


<210>  66
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is arginine, ornithine, or lysine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is arginine, ornithine, or lysine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is arginine, ornithine, or lysine

<400>  66

Gly Ile Val Asp Glu Cys Cys His Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Gln Met Tyr Cys Asn 
            20      


<210>  67
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa at position 7 is ornithine, lysine or arginine

<400>  67

Gly Ala Gly Ser Ser Ser Xaa Arg Ala Pro Gln Thr 
1               5                   10          


<210>  68
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine or asparagine

<400>  68

Gly Ile Val Glu Gln Cys Cys His Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Xaa 
            20      


<210>  69
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine or phenylalnine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is arginine, lysine, ornithine or alanine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is arginine, lysine, ornithine or alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is arginine, lysine, ornithine or leucine

<220>
<221>  MISC_FEATURE
<222>  (17)..(17)
<223>  Xaa at position 17 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is methionine, asparagine or threonine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine or asparagine

<400>  69

Gly Ile Val Asp Glu Cys Cys Xaa Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Xaa Xaa Xaa Cys Xaa 
            20      


<210>  70
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(6)
<223>  Xaas at positions 3-6 are independently any amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  Xaas at positions 7-8 are independently arginine, lysine or 
       ornithine



<400>  70

Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa 
1               5               


<210>  71
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  The Xaa at position 18 is ornithine

<400>  71

His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Asp Xaa Gly Phe Tyr 
            20      


<210>  72
<211>  24
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is ornithine

<400>  72

Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr 
            20                  


<210>  73
<211>  29
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is ornithine

<400>  73

Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Pro Lys Thr 
            20                  25                  


<210>  74
<211>  29
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Xaa at position 21 is ornithine
<400>  74

Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Lys Pro Thr 
            20                  25                  


<210>  75
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  The Xaa at position 9 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (14)..(15)
<223>  The Xaas at positions 14 and 15 are both ornithine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  The Xaa at position 19 is tyrosine, 4-methoxy-phenylalanine or 
       4-amino phenylalanine

<400>  75

Gly Ile Val Asp Glu Cys Cys His Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Gln Met Xaa Cys Asn 
            20      


<210>  76
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  76

Phe Asn Lys Pro Thr 
1               5   


<210>  77
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  77

Phe Asn Lys Pro 
1               


<210>  78
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  78

Tyr Thr Pro Lys 
1               


<210>  79
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin B chain derivative sequence

<400>  79

Phe Asn Pro Lys 
1               


<210>  80
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin B chain derivative sequence

<400>  80

Tyr Thr Lys Pro Thr 
1               5   


<210>  81
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  insulin B chain derivative sequence

<400>  81

Phe Asn Pro Lys Thr 
1               5   


<210>  82
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  82

Ala Pro Gln Thr 
1               


<210>  83
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is any non-aromatic amino acid

<400>  83

Gly Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10          


<210>  84
<211>  4
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  The Xaa at position 1 is phenylalanine or desamino-phenylalanine

<400>  84

Xaa Val Asn Gln 
1               


<210>  85
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  The Xaas at positions 7 and 8 are independently arginine, lysine 
       or ornithine

<400>  85

Gly Tyr Gly Ser Ser Ser Xaa Xaa 
1               5               


<210>  86
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  The Xaas at positions 1-7 are independently any amino acid

<400>  86

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5                   10              


<210>  87
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  The Xaas at positions 1-7 are independently any amino acid

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  The Xaa at positions 13 is arginine, lysine or ornithine

<400>  87

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Ser Ser Ser Arg Xaa Ala Pro Gln 
1               5                   10                  15      


Thr 
    


<210>  88
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  The Xaa at position 19 is tyrosine, 4-methoxy-phenylalanine or 
       4-amino phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is alanine, glycine or asparagine

<400>  88

Gly Ile Val Asp Glu Cys Cys His Arg Ser Cys Asp Leu Arg Arg Leu 
1               5                   10                  15      


Glu Met Xaa Cys Xaa 
            20      


<210>  89
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  89

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Pro Lys Thr 
            20                  25                  


<210>  90
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is any amino acid other than tyrosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  90

Gly Xaa Gly Ser Ser Ser Xaa Arg 
1               5               


<210>  91
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  The Xaa at position 7 is arginine, lysine or ornithine

<400>  91

Gly Tyr Gly Ser Ser Ser Xaa Arg 
1               5               


<210>  92
<211>  35
<212>  PRT
<213>  Homo sapiens

<400>  92

Arg Arg Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly 
1               5                   10                  15      


Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu 
            20                  25                  30          


Gln Lys Arg 
        35  


<210>  93
<211>  51
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  The Xaa at position 22 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (39)..(39)
<223>  The Xaa at position 39 is ornithine

<220>
<221>  MISC_FEATURE
<222>  (44)..(45)
<223>  The Xaa at positions 44-45 are ornithine

<400>  93

Cys Gly Pro Glu His Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Lys Pro Thr Gly Ile 
            20                  25                  30          


Val Asp Glu Cys Cys Phe Xaa Ser Cys Asp Leu Xaa Xaa Leu Glu Asn 
        35                  40                  45              


Tyr Cys Asn 
    50      


<210>  94
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  The Xaas at positions 4-7 are independently any amino acid

<400>  94

Phe Xaa Ala Xaa Xaa Xaa Xaa Arg Arg 
1               5                   


<210>  95
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  The Xaas at positions 4-7 are independently any amino acid

<400>  95

Phe Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala 
1               5                   10  


<210>  96
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  The Xaas at positions 4-7 are independently any amino acid

<400>  96

Phe Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro 
1               5                   10      


<210>  97
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  The Xaas at positions 4-7 are independently any amino acid

<400>  97

Phe Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln 
1               5                   10          


<210>  98
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  The Xaas at positions 4-7 are independently any amino acid

<400>  98

Phe Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln Thr 
1               5                   10              


<210>  99
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  The Xaas at positions 5-8 are independently any amino acid

<400>  99

Phe Asn Xaa Ala Xaa Xaa Xaa Xaa Arg Arg 
1               5                   10  


<210>  100
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  The Xaas at positions 5-8 are independently any amino acid

<400>  100

Phe Asn Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala 
1               5                   10      


<210>  101
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  The Xaas at positions 5-8 are independently any amino acid

<400>  101

Phe Asn Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro 
1               5                   10          


<210>  102
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  The Xaas at positions 5-8 are independently any amino acid

<400>  102

Phe Asn Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln 
1               5                   10              


<210>  103
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(8)
<223>  The Xaas at positions 5-8 are independently any amino acid

<400>  103

Phe Asn Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln Thr 
1               5                   10                  


<210>  104
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  The Xaas at positions 6-9 are independently any amino acid


<400>  104

Phe Asn Lys Xaa Ala Xaa Xaa Xaa Xaa Arg Arg 
1               5                   10      


<210>  105
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  The Xaas at positions 6-9 are independently any amino acid


<400>  105

Phe Asn Lys Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala 
1               5                   10          


<210>  106
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  The Xaas at positions 6-9 are independently any amino acid

<400>  106

Phe Asn Lys Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro 
1               5                   10              


<210>  107
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  The Xaas at positions 6-9 are independently any amino acid

<400>  107

Phe Asn Lys Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln 
1               5                   10                  


<210>  108
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  The Xaas at positions 6-9 are independently any amino acid


<400>  108

Phe Asn Lys Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln Thr 
1               5                   10                  15  


<210>  109
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  The Xaas at positions 7-10 are independently any amino acid

<400>  109

Phe Asn Lys Pro Xaa Ala Xaa Xaa Xaa Xaa Arg Arg 
1               5                   10          


<210>  110
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  The Xaas at positions 7-10 are independently any amino acid

<400>  110

Phe Asn Lys Pro Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala 
1               5                   10              


<210>  111
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  The Xaas at positions 7-10 are independently any amino acid


<400>  111

Phe Asn Lys Pro Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro 
1               5                   10                  


<210>  112
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  The Xaas at positions 7-10 are independently any amino acid


<400>  112

Phe Asn Lys Pro Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln 
1               5                   10                  15  


<210>  113
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (7)..(10)
<223>  The Xaas at positions 7-10 are independently any amino acid


<400>  113

Phe Asn Lys Pro Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln Thr 
1               5                   10                  15      


<210>  114
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (8)..(11)
<223>  The Xaas at positions 8-11 are independently any amino acid


<400>  114

Phe Asn Lys Pro Thr Xaa Ala Xaa Xaa Xaa Xaa Arg Arg 
1               5                   10              


<210>  115
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (8)..(11)
<223>  The Xaas at positions 8-11 are independently any amino acid


<400>  115

Phe Asn Lys Pro Thr Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala 
1               5                   10                  


<210>  116
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (8)..(11)
<223>  The Xaas at positions 8-11 are independently any amino acid


<400>  116

Phe Asn Lys Pro Thr Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro 
1               5                   10                  15  


<210>  117
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (8)..(11)
<223>  The Xaas at positions 8-11 are independently any amino acid

<400>  117

Phe Asn Lys Pro Thr Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln 
1               5                   10                  15      


<210>  118
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (8)..(11)
<223>  The Xaas at positions 8-11 are independently any amino acid

<400>  118

Phe Asn Lys Pro Thr Xaa Ala Xaa Xaa Xaa Xaa Arg Arg Ala Pro Gln 
1               5                   10                  15      


Thr 
    


<210>  119
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is any amino aicd other than tyrosine

<400>  119

Phe Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5                   


<210>  120
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is any amino aicd other than tyrosine

<400>  120

Phe Xaa Xaa Gly Ser Ser Ser Arg Arg Ala 
1               5                   10  


<210>  121
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is any amino aicd other than tyrosine

<400>  121

Phe Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10      


<210>  122
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is any amino aicd other than tyrosine

<400>  122

Phe Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10          


<210>  123
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  The Xaa at position 2 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is any amino aicd other than tyrosine

<400>  123

Phe Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10              


<210>  124
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is any amino aicd other than tyrosine


<400>  124

Phe Asn Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5                   10  


<210>  125
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is any amino aicd other than tyrosine
<400>  125

Phe Asn Xaa Xaa Gly Ser Ser Ser Arg Arg Ala 
1               5                   10      


<210>  126
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is any amino aicd other than tyrosine

<400>  126

Phe Asn Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10          


<210>  127
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is any amino aicd other than tyrosine


<400>  127

Phe Asn Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10              


<210>  128
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  The Xaa at position 3 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is any amino aicd other than tyrosine

<400>  128

Phe Asn Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10                  


<210>  129
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is any amino aicd other than tyrosine


<400>  129

Phe Asn Lys Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5                   10      


<210>  130
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is any amino aicd other than tyrosine


<400>  130

Phe Asn Lys Xaa Xaa Gly Ser Ser Ser Arg Arg Ala 
1               5                   10          


<210>  131
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is any amino aicd other than tyrosine

<400>  131

Phe Asn Lys Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10              


<210>  132
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is any amino aicd other than tyrosine


<400>  132

Phe Asn Lys Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10                  


<210>  133
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  The Xaa at position 4 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is any amino aicd other than tyrosine


<400>  133

Phe Asn Lys Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10                  15  


<210>  134
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  134

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5                   10          


<210>  135
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  135

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala 
1               5                   10              


<210>  136
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  136

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10                  


<210>  137
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  137

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10                  15  


<210>  138
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  138

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10                  15      


<210>  139
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  139

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg 
1               5                   10          


<210>  140
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  140

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala 
1               5                   10              


<210>  141
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  141

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro 
1               5                   10                  


<210>  142
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  142

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln 
1               5                   10                  15  


<210>  143
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  The Xaa at position 5 is glycine, alanine, valine, leucine, 
       isoleucine, proline, phenylalanine or methionine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is any amino aicd other than tyrosine

<400>  143

Phe Asn Lys Pro Xaa Xaa Gly Ser Ser Ser Arg Arg Ala Pro Gln Thr 
1               5                   10                  15      


<210>  144
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  144

Thr Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5                   


<210>  145
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide

<400>  145

Lys Thr Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5                   10  


<210>  146
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide

<400>  146

Pro Lys Thr Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5                   10      


<210>  147
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide

<400>  147

Thr Pro Lys Thr Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5                   10          


<210>  148
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide

<400>  148

Thr Lys Pro Thr Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5                   10          


<210>  149
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide

<400>  149

Ser Arg Pro Ala Gly Tyr Gly Ser Ser Ser Arg Arg 
1               5                   10          


<210>  150
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is alanine, glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or or cysteic acid

<400>  150

Xaa Leu Cys Gly Xaa Xaa Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  151
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa at position 4 is aspartic acid or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine, threonine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is serine, arginine, ornithine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is tyrosine, arginine, ornathine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is glutamine, glutamic acid, arginine, 
       ornithine, alanine, lysine or leucine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine


<400>  151

Gly Ile Val Xaa Glu Cys Cys Xaa Xaa Ser Cys Asp Leu Xaa Xaa Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Asn 
            20      


<210>  152
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa at position 4 is aspartic acid or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa at position 5 is glutamine or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine, threonine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is serine, arginine, ornithine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa at position 10 is isoleucine or serine

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa at position 12 is aspartic acid or serine

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is tyrosine, arginine, ornathine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  Xaa at position 15 is glutamine, glutamic acid, arginine, 
       ornithine, alanine, lysine or leucine

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is methionine, asparagine, glutamine, glutamic
       acid, aspartic acid or threonine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine, serine, valine, 
       threonine, isoleucine, leucine, glutamine, glutamic acid, 
       asparagine, aspartic acid, histidine, tryptophan, tyrosine, or 
       methionine

<400>  152

Gly Ile Val Xaa Xaa Cys Cys Xaa Xaa Xaa Cys Xaa Leu Xaa Xaa Leu 
1               5                   10                  15      


Glu Xaa Xaa Cys Xaa 
            20      


<210>  153
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking pepetide


<220>
<221>  MISC_FEATURE
<222>  (2)..(6)
<223>  the Xaas at positions 2-6 are independently glutamic acid or 
       aspartic acid

<400>  153

Gly Xaa Xaa Xaa Xaa Xaa Lys 
1               5           


<210>  154
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  The Xaa at position 6 is histidine, aspartic acid, glutamic acid,
       homocysteic acid or cysteic acid

<400>  154

His Leu Cys Gly Ser Xaa Leu Val Glu Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Glu Arg Gly Phe Phe 
            20      


<210>  155
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine or 4-amino phenylanaline

<400>  155

Gly Ile Val Asp Glu Cys Cys His Arg Ser Cys Asp Leu Arg Arg Leu 
1               5                   10                  15      


Glu Met Xaa Cys Ala 
            20      


<210>  156
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide for testing cleavage of dipeptide prodrug 
       element

<400>  156

His Ser Arg Gly Thr Phe 
1               5       


<210>  157
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide for testing cleavage of dipeptide prodrug 
       element


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Sarcosine

<400>  157

Lys Xaa His Ser Thr Gly Thr Phe 
1               5               


<210>  158
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glucagon analogue


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa at position 1 is d-histidine

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa at position 2 is d-threonine

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa at position 3 is d-arginine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Xaa at position 5 is d-threonine

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Xaa at position 6 is d-phenylalanine

<400>  158

Xaa Xaa Xaa Gly Xaa Xaa 
1               5       


<210>  159
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glucagon analogue


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa at position 1 is d-lysine

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa at position 2 is d-sarcosine

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa at position 3 is d-histidine

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa at position 4 is d-threonine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Xaa at position 5 is d-arginine

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Xaa at position 7 is d-threonine

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Xaa at position 8 is d-phenylalanine

<400>  159

Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa 
1               5               


<210>  160
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide for testing cleavage of dipeptide prodrug 
       element


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  The Xaa at position 1 is histidine, d-histidine or 
       N-methyl-histdine

<400>  160

Xaa Ser Arg Gly Thr Phe 
1               5       


<210>  161
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  The Xaas at positions 1-3 are independently any amino acid

<400>  161

Xaa Xaa Xaa Thr 
1               


<210>  162
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  analog of IGF B chain sequence


<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  The Xaa at position 12 is aspartic acid or glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  The Xaa at position 21 is ornithine, lysine or arginine

<400>  162

Gly Pro Glu His Leu Cys Gly Ser His Leu Val Xaa Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr 
            20                  


<210>  163
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linking peptide


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  The Xaas at positions 7 and 8 are independently arginine, lysine 
       or ornithine

<400>  163

Gly Ala Gly Ser Ser Ser Xaa Xaa 
1               5               


<210>  164
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine, threonine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine, serine, valine, 
       threonine, isoleucine, leucine, glutamine, glutamic acid, 
       asparagine, aspartic acid, histidine, tryptophan, tyrosine, or 
       methionine

<400>  164

Gly Ile Val Glu Gln Cys Cys Xaa Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Xaa 
            20      


<210>  165
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is histidine, aspartic acid, glutamic acid, 
       homocysteic acid or cysteic acid

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa at position 18 is alanine, ornithine, lysine or arginine

<400>  165

Xaa Leu Cys Gly Ala Xaa Leu Val Asp Ala Leu Tyr Leu Val Cys Gly 
1               5                   10                  15      


Asp Xaa Gly Phe Tyr Phe Asn 
            20              


<210>  166
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine, threonine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa at position 9 is serine, arginine, ornithine, lysine or 
       alanine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine, 4-methoxy phenylalanine  or 
       4-amino-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, glycine, serine, valine, 
       threonine, isoleucine, leucine, glutamine, glutamic acid, 
       asparagine, aspartic acid, histidine, tryptophan, tyrosine, or 
       methionine

<400>  166

Gly Ile Val Asp Glu Cys Cys Xaa Xaa Ser Cys Asp Leu Arg Arg Leu 
1               5                   10                  15      


Glu Met Xaa Cys Xaa 
            20      


<210>  167
<211>  13
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is ornithine, lysine or arginine

<400>  167

Gly Ala Gly Ser Ser Ser Arg Xaa Arg Ala Pro Gln Thr 
1               5                   10              


<210>  168
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  168

Gly Pro Glu Thr Leu Cys Gly Ala Glu Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Arg Gly Phe Tyr Phe Asn Lys Pro Thr 
            20                  25                  


<210>  169
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  169

Gly Glu Glu Glu Glu Glu Glu Arg Lys 
1               5                   


<210>  170
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  170

Gly Glu Glu Glu Glu Glu Arg Lys 
1               5               


<210>  171
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  171

Gly Glu Glu Glu Glu Arg Lys 
1               5           


<210>  172
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  172

Gly Glu Glu Glu Arg Lys 
1               5       


<210>  173
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  173

Gly Glu Glu Arg Lys 
1               5   


<210>  174
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  174

Gly Glu Arg Lys 
1               


<210>  175
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  175

Gly Glu Glu Glu Glu Glu Glu Arg Lys 
1               5                   


<210>  176
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  176

Gly Glu Glu Glu Glu Glu Glu Lys 
1               5               


<210>  177
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  177

Gly Glu Glu Glu Glu Glu Lys 
1               5           


<210>  178
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  178

Gly Glu Glu Glu Glu Lys 
1               5       


<210>  179
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  179

Gly Glu Glu Lys 
1               


<210>  180
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  180

Gly Asp Asp Asp Asp Asp Asp Arg Lys 
1               5                   


<210>  181
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  181

Gly Asp Asp Asp Asp Asp Arg Lys 
1               5               


<210>  182
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  182

Gly Asp Asp Asp Asp Arg Lys 
1               5           


<210>  183
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  183

Gly Asp Asp Asp Arg Lys 
1               5       


<210>  184
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  184

Gly Asp Asp Arg Lys 
1               5   


<210>  185
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  185

Gly Asp Arg Lys 
1               


<210>  186
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  186

Gly Asp Asp Asp Asp Asp Asp Lys 
1               5               


<210>  187
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  187

Gly Asp Asp Asp Asp Asp Lys 
1               5           


<210>  188
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  188

Gly Asp Asp Asp Asp Lys 
1               5       


<210>  189
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  189

Gly Asp Asp Asp Lys 
1               5   


<210>  190
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide linker

<400>  190

Gly Asp Asp Lys 
1               


<210>  191
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(7)
<223>  Xaa at positions 2-7 are independently glutamic acid or aspartic 
       acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(7)
<223>  any or all of amino acids at positions 3-7 are present or absent

<400>  191

Gly Xaa Xaa Xaa Xaa Xaa Xaa Lys 
1               5               


<210>  192
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa at position 1 is glycine, glutamic acid or aspartic acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(7)
<223>  Xaa at positions 2-7 are independently glutamic acid or aspartic 
       acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  any or all of amino acids at positions 4-7 are present or absent

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is arginine, glutamic acid or aspartic acid

<400>  192

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Lys 
1               5                   


<210>  193
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal extension peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(7)
<223>  Xaa at positions 2-7 are independently glutamic acid or aspartic 
       acid

<400>  193

Gly Xaa Xaa Xaa Xaa Xaa Xaa Lys 
1               5               


<210>  194
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position 19 is tyrosine or 4-amino phenylanaline

<400>  194

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Asn 
            20      


<210>  195
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin A chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is histidine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa at position19 is tyrosine or 4-amino-phenylalanine

<400>  195

Gly Ile Val Asp Glu Cys Cys Xaa Arg Ser Cys Asp Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Xaa Cys Asn 
            20      


<210>  196
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa at position 4 is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, ornithine, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Xaa at position 24 is tyrosine, histidine, asparagine or phenylalanine


<400>  196

Gly Pro Glu Xaa Leu Cys Gly Ser His Leu Val Asp Ala Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Xaa 
            20                  

<210>  197
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Analog of the insulin B chain


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa at position 8 is alanine or an alpha, alpha disubstituted 
       amino acid

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is alanine or or an alpha, alpha disubsituted 
       amino acid

<220>
<221>  MISC_FEATURE
<222>  (21)..(21)
<223>  Xaa at position 21 is alanine, lysine, ornithine and arginine

<400>  197

Gly Pro Glu Thr Leu Cys Gly Xaa Glu Leu Val Asp Xaa Leu Tyr Leu 
1               5                   10                  15      


Val Cys Gly Asp Xaa Gly Phe Tyr Phe Asn Lys Pro Thr 
            20                  25                  


