                         SEQUENCE LISTING

<110>  UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
       MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
       BOONS, Geert-Jan
       WOLFERT, Margaretha
       GENDLER, Sandra J.
       LAKSHMINARAYANAN, Vani
       COHEN, Peter A.
 
<120>  IMMUNOGENIC VACCINE

<130>  235.01620201

<140>  PCT/US2011/040037
<141>  2011-06-10

<150>  US 61/354,076
<151>  2010-06-11

<150>  US 13/002,180
<151>  2010-12-30

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  tetanus toxin

<400>  1

Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu 
1               5                   10                  15  


<210>  2
<211>  20
<212>  PRT
<213>  Neisseria meningitides

<400>  2

Tyr Ala Phe Lys Tyr Ala Arg His Ala Asn Val Gly Arg Asn Ala Phe 
1               5                   10                  15      


Glu Leu Phe Leu 
            20  


<210>  3
<211>  13
<212>  PRT
<213>  polio virus

<400>  3

Lys Leu Phe Ala Val Trp Lys Ile Thr Tyr Lys Asp Thr 
1               5                   10              


<210>  4
<211>  15
<212>  PRT
<213>  tetanus toxin

<400>  4

Val Ser Ile Asp Lys Phe Arg Ile Phe Cys Lys Ala Asn Pro Lys 
1               5                   10                  15  


<210>  5
<211>  16
<212>  PRT
<213>  tetanus toxin

<400>  5

Leu Lys Phe Ile Ile Lys Arg Tyr Thr Pro Asn Asn Glu Ile Asp Ser 
1               5                   10                  15      


<210>  6
<211>  16
<212>  PRT
<213>  tetanus toxin

<400>  6

Ile Arg Glu Asp Asn Asn Ile Thr Leu Lys Leu Asp Arg Cys Asn Asn 
1               5                   10                  15      


<210>  7
<211>  21
<212>  PRT
<213>  tetanus toxin

<400>  7

Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val Ser 
1               5                   10                  15      


Ala Ser His Leu Glu 
            20      


<210>  8
<211>  20
<212>  PRT
<213>  Neisseria meningitidis

<400>  8

Tyr Ala Phe Lys Tyr Ala Arg His Ala Asn Val Gly Arg Asn Ala Phe 
1               5                   10                  15      


Glu Leu Phe Leu 
            20  


<210>  9
<211>  18
<212>  PRT
<213>  Pseudomonas falsiparum

<400>  9

Glu Lys Lys Ile Ala Lys Met Glu Lys Ala Ser Ser Val Phe Asn Val 
1               5                   10                  15      


Asn Asn 
        


<210>  10
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  peptide sequence modified by O-GlcNAc

<400>  10

Thr Pro Val Ser Ser 
1               5   


<210>  11
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  peptide sequence modified by O-GlcNAc

<400>  11

Gly Ser Thr Pro Val Ser Ser Ala Asn Met 
1               5                   10  


<210>  12
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  peptide sequence modified by O-GlcNAc

<400>  12

Cys Gly Ser Thr Pro Val Ser Ser Ala Asn Met 
1               5                   10      


<210>  13
<211>  54
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  13
ccccatgtat ccatatgacg tcccagacta tgccgcgtct tccgtgggca acgt             54


<210>  14
<211>  74
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  14
ggggacaagt ttgtacaaaa aagcaggctg gatgatgtat ccatatgacg tcccagacta       60

tgccgcgtct tccg                                                         74


<210>  15
<211>  70
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  15
ggggaccact ttgtacaaga aagctgggtt ctatgctgac tcagtgactt caacgggctt       60

aatcatgtgg                                                              70


<210>  16
<211>  54
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  16
ccccatgtat ccatatgacg tcccagacta tgccgtgcag aaggagagtc aagc             54


<210>  17
<211>  74
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  17
ggggacaagt ttgtacaaaa aagcaggctg gatgatgtat ccatatgacg tcccagacta       60

tgccgtgcag aagg                                                         74


<210>  18
<211>  50
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  18
ggggaccact ttgtacaaga aagctgggtt cacaggctcc gaccaagtat                  50


<210>  19

<400>  19
000

<210>  20
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  B-cell peptide epitope

<400>  20

Ser Ala Pro Asp Thr Arg Pro Ala Pro 
1               5                   


<210>  21
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  B-cell peptide epitope

<400>  21

Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
1               5                   10  


<210>  22
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  B-cell peptide epitope

<400>  22

Ser Ala Pro Asp Thr Arg Pro Leu 
1               5               


<210>  23
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  B-cell peptide epitope

<400>  23

Thr Ser Ala Pro Asp Thr Arg Pro Leu 
1               5                   


<210>  24
<211>  13
<212>  PRT
<213>  artificial

<220>
<223>  T-cell pan DR epitope PADRE sequence

<400>  24

Ala Lys Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala 
1               5                   10              


<210>  25
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  T-cell pan DR epitope PADRE sequence

<400>  25

Phe Val Ala Ala Trp Thr Leu Lys Ala Ala Ala 
1               5                   10      


<210>  26
<211>  15
<212>  PRT
<213>  artificial

<220>
<223>  contiguous 15-mer amino acid sequence of MUC1 epitope

<400>  26

Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala 
1               5                   10                  15  


<210>  27
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  contiguous 21-mer amino acid sequence of MUC1 epitope

<400>  27

Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro 
1               5                   10                  15      


Asp Thr Arg Pro Leu 
            20      


<210>  28
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  contiguous 21-mer amino acid sequence of MUC1 epitope with single
       glycosylation site

<400>  28

Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro 
1               5                   10                  15      


Asp Thr Arg Pro Leu 
            20      


<210>  29
<211>  21
<212>  PRT
<213>  artificial

<220>
<223>  contiguous 21-mer amino acid sequence of MUC1 epitope with double
       glycosylation sites

<400>  29

Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro 
1               5                   10                  15      


Asp Thr Arg Pro Leu 
            20      


<210>  30
<211>  27
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope


<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be any naturally occurring amino acid

<400>  30

Ser Lys Lys Lys Lys Gly Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
1               5                   10                  15      


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Xaa 
            20                  25          


<210>  31
<211>  25
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope

<400>  31

Ser Lys Lys Lys Lys Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr 
1               5                   10                  15      


Ser Ala Pro Asp Thr Arg Pro Ala Pro 
            20                  25  


<210>  32
<211>  23
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope

<400>  32

Ser Lys Lys Lys Lys Gly Ser Leu Ser Tyr Thr Asn Pro Ala Val Ala 
1               5                   10                  15      


Ala Ala Thr Ala Ser Asn Leu 
            20              


<210>  33
<211>  31
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope

<400>  33

Ser Lys Lys Lys Lys Gly Cys Lys Leu Phe Ala Val Trp Lys Ile Thr 
1               5                   10                  15      


Tyr Lys Asp Thr Gly Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
            20                  25                  30      


<210>  34
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope

<400>  34

Ser Lys Lys Lys Lys Gly Cys Lys Leu Phe Ala Val Trp Lys Ile Thr 
1               5                   10                  15      


Tyr Lys Asp Thr 
            20  


<210>  35
<211>  26
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope

<400>  35

Gly Gly Lys Leu Phe Ala Val Trp Lys Ile Thr Tyr Lys Asp Thr Gly 
1               5                   10                  15      


Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
            20                  25      


<210>  36
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  CpG immunostimulatory oliodeoxynucleotide

<400>  36
tccatgacgt tcctgacgtt                                                   20


<210>  37
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  MUC1 epitope

<400>  37

Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro 
1               5                   10                  15      


Asp Thr Arg Pro Ala Pro 
            20          


<210>  38
<211>  240
<212>  PRT
<213>  Homo sapiens

<400>  38

Met Thr Pro Gly Thr Gln Ser Pro Phe Phe Leu Leu Leu Leu Leu Thr 
1               5                   10                  15      


Val Leu Thr Val Val Thr Gly Ser Gly His Ala Ser Ser Thr Pro Gly 
            20                  25                  30          


Gly Glu Lys Glu Thr Ser Ala Thr Gln Arg Ser Ser Val Pro Ser Ser 
        35                  40                  45              


Thr Glu Lys Asn Ala Val Ser Met Thr Ser Ser Val Leu Ser Ser His 
    50                  55                  60                  


Ser Pro Gly Ser Gly Ser Ser Thr Thr Gln Gly Gln Asp Val Thr Leu 
65                  70                  75                  80  


Ala Pro Ala Thr Glu Pro Ala Ser Gly Ser Ala Ala Thr Trp Gly Gln 
                85                  90                  95      


Asp Val Thr Ser Val Pro Val Thr Arg Pro Ala Leu Gly Ser Thr Thr 
            100                 105                 110         


Pro Pro Ala His Asp Val Thr Ser Ala Pro Asp Asn Lys Pro Ala Pro 
        115                 120                 125             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    130                 135                 140                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
145                 150                 155                 160 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                165                 170                 175     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            180                 185                 190         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        195                 200                 205             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    210                 215                 220                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
225                 230                 235                 240 


<210>  39
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  missynthesized 18mer C terminus control peptide

<400>  39

Ser Ser Leu Ser Tyr Asn Thr Asn Pro Ala Val Ala Ala Ala Ser Ala 
1               5                   10                  15      


Asn Leu 
        


