﻿SEQUENCE LISTING

<110>   GENE BRIDGES GmbH

<120>   Direct cloning

<130>   P054911WO

<141>   

<150>   GB1009732.7
<151>   2010-06-10

<160>   2

<170>   SeqWin2010, version 1.0

<210>   1
<211>   866
<212>   PRT
<213>   Escherichia coli K12

<400>   1
Met Ser Thr Lys Pro Leu Phe Leu Leu Arg Lys Ala Lys Lys Ser Ser 
1               5                   10                  15      

Gly Glu Pro Asp Val Val Leu Trp Ala Ser Asn Asp Phe Glu Ser Thr 
            20                  25                  30          

Cys Ala Thr Leu Asp Tyr Leu Ile Val Lys Ser Gly Lys Lys Leu Ser 
        35                  40                  45              

Ser Tyr Phe Lys Ala Val Ala Thr Asn Phe Pro Val Val Asn Asp Leu 
    50                  55                  60                  

Pro Ala Glu Gly Glu Ile Asp Phe Thr Trp Ser Glu Arg Tyr Gln Leu 
65                  70                  75                  80  

Ser Lys Asp Ser Met Thr Trp Glu Leu Lys Pro Gly Ala Ala Pro Asp 
                85                  90                  95      

Asn Ala His Tyr Gln Gly Asn Thr Asn Val Asn Gly Glu Asp Met Thr 
            100                 105                 110         

Glu Ile Glu Glu Asn Met Leu Leu Pro Ile Ser Gly Gln Glu Leu Pro 
        115                 120                 125             

Ile Arg Trp Leu Ala Gln His Gly Ser Glu Lys Pro Val Thr His Val 
    130                 135                 140                 

Ser Arg Asp Gly Leu Gln Ala Leu His Ile Ala Arg Ala Glu Glu Leu 
145                 150                 155                 160 

Pro Ala Val Thr Ala Leu Ala Val Ser His Lys Thr Ser Leu Leu Asp 
                165                 170                 175     

Pro Leu Glu Ile Arg Glu Leu His Lys Leu Val Arg Asp Thr Asp Lys 
            180                 185                 190         

Val Phe Pro Asn Pro Gly Asn Ser Asn Leu Gly Leu Ile Thr Ala Phe 
        195                 200                 205             

Phe Glu Ala Tyr Leu Asn Ala Asp Tyr Thr Asp Arg Gly Leu Leu Thr 
    210                 215                 220                 

Lys Glu Trp Met Lys Gly Asn Arg Val Ser His Ile Thr Arg Thr Ala 
225                 230                 235                 240 

Ser Gly Ala Asn Ala Gly Gly Gly Asn Leu Thr Asp Arg Gly Glu Gly 
                245                 250                 255     

Phe Val His Asp Leu Thr Ser Leu Ala Arg Asp Val Ala Thr Gly Val 
            260                 265                 270         

Leu Ala Arg Ser Met Asp Leu Asp Ile Tyr Asn Leu His Pro Ala His 
        275                 280                 285             

Ala Lys Arg Ile Glu Glu Ile Ile Ala Glu Asn Lys Pro Pro Phe Ser 
    290                 295                 300                 

Val Phe Arg Asp Lys Phe Ile Thr Met Pro Gly Gly Leu Asp Tyr Ser 
305                 310                 315                 320 

Arg Ala Ile Val Val Ala Ser Val Lys Glu Ala Pro Ile Gly Ile Glu 
                325                 330                 335     

Val Ile Pro Ala His Val Thr Glu Tyr Leu Asn Lys Val Leu Thr Glu 
            340                 345                 350         

Thr Asp His Ala Asn Pro Asp Pro Glu Ile Val Asp Ile Ala Cys Gly 
        355                 360                 365             

Arg Ser Ser Ala Pro Met Pro Gln Arg Val Thr Glu Glu Gly Lys Gln 
    370                 375                 380                 

Asp Asp Glu Glu Lys Pro Gln Pro Ser Gly Thr Thr Ala Val Glu Gln 
385                 390                 395                 400 

Gly Glu Ala Glu Thr Met Glu Pro Asp Ala Thr Glu His His Gln Asp 
                405                 410                 415     

Thr Gln Pro Leu Asp Ala Gln Ser Gln Val Asn Ser Val Asp Ala Lys 
            420                 425                 430         

Tyr Gln Glu Leu Arg Ala Glu Leu His Glu Ala Arg Lys Asn Ile Pro 
        435                 440                 445             

Ser Lys Asn Pro Val Asp Asp Asp Lys Leu Leu Ala Ala Ser Arg Gly 
    450                 455                 460                 

Glu Phe Val Asp Gly Ile Ser Asp Pro Asn Asp Pro Lys Trp Val Lys 
465                 470                 475                 480 

Gly Ile Gln Thr Arg Asp Cys Val Tyr Gln Asn Gln Pro Glu Thr Glu 
                485                 490                 495     

Lys Thr Ser Pro Asp Met Asn Gln Pro Glu Pro Val Val Gln Gln Glu 
            500                 505                 510         

Pro Glu Ile Ala Cys Asn Ala Cys Gly Gln Thr Gly Gly Asp Asn Cys 
        515                 520                 525             

Pro Asp Cys Gly Ala Val Met Gly Asp Ala Thr Tyr Gln Glu Thr Phe 
    530                 535                 540                 

Asp Glu Glu Ser Gln Val Glu Ala Lys Glu Asn Asp Pro Glu Glu Met 
545                 550                 555                 560 

Glu Gly Ala Glu His Pro His Asn Glu Asn Ala Gly Ser Asp Pro His 
                565                 570                 575     

Arg Asp Cys Ser Asp Glu Thr Gly Glu Val Ala Asp Pro Val Ile Val 
            580                 585                 590         

Glu Asp Ile Glu Pro Gly Ile Tyr Tyr Gly Ile Ser Asn Glu Asn Tyr 
        595                 600                 605             

His Ala Gly Pro Gly Ile Ser Lys Ser Gln Leu Asp Asp Ile Ala Asp 
    610                 615                 620                 

Thr Pro Ala Leu Tyr Leu Trp Arg Lys Asn Ala Pro Val Asp Thr Thr 
625                 630                 635                 640 

Lys Thr Lys Thr Leu Asp Leu Gly Thr Ala Phe His Cys Arg Val Leu 
                645                 650                 655     

Glu Pro Glu Glu Phe Ser Asn Arg Phe Ile Val Ala Pro Glu Phe Asn 
            660                 665                 670         

Arg Arg Thr Asn Ala Gly Lys Glu Glu Glu Lys Ala Phe Leu Met Glu 
        675                 680                 685             

Cys Ala Ser Thr Gly Lys Thr Val Ile Thr Ala Glu Glu Gly Arg Lys 
    690                 695                 700                 

Ile Glu Leu Met Tyr Gln Ser Val Met Ala Leu Pro Leu Gly Gln Trp 
705                 710                 715                 720 

Leu Val Glu Ser Ala Gly His Ala Glu Ser Ser Ile Tyr Trp Glu Asp 
                725                 730                 735     

Pro Glu Thr Gly Ile Leu Cys Arg Cys Arg Pro Asp Lys Ile Ile Pro 
            740                 745                 750         

Glu Phe His Trp Ile Met Asp Val Lys Thr Thr Ala Asp Ile Gln Arg 
        755                 760                 765             

Phe Lys Thr Ala Tyr Tyr Asp Tyr Arg Tyr His Val Gln Asp Ala Phe 
    770                 775                 780                 

Tyr Ser Asp Gly Tyr Glu Ala Gln Phe Gly Val Gln Pro Thr Phe Val 
785                 790                 795                 800 

Phe Leu Val Ala Ser Thr Thr Ile Glu Cys Gly Arg Tyr Pro Val Glu 
                805                 810                 815     

Ile Phe Met Met Gly Glu Glu Ala Lys Leu Ala Gly Gln Gln Glu Tyr 
            820                 825                 830         

His Arg Asn Leu Arg Thr Leu Ser Asp Cys Leu Asn Thr Asp Glu Trp 
        835                 840                 845             

Pro Ala Ile Lys Thr Leu Ser Leu Pro Arg Trp Ala Lys Glu Tyr Ala 
    850                 855                 860                 

Asn Asp 
865     

<210>   2
<211>   30
<212>   DNA
<213>   Artificial Sequence

<220>   
<223>   I-SceI recognition site

<400>   2
agttacgcta gggataacag ggtaatatag                                   30

