Sequence listing

<110>  Pieris AG
 
<120>  Tear lipocalin muteins binding to IL-4 R alpha

<130>  PIE14083PCT

<150>  US 61/352 461
<151>  2010-06-08

<160>  20    

<170>  PatentIn version 3.5


<210>  1
<211>  3700
<212>  DNA
<213>  Artificial

<220>
<223>  Expression vector pTLPC10

<400>  1

ccatcgaatg gccagatgat taattcctaa tttttgttga cactctatca ttgatagagt     60

tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct    120

agataacgag ggcaaaaaat gaaaaagaca gctatcgcga ttgcagtggc actggctggt    180

ttcgctaccg tagcgcaggc cgacgcatcg atgaccggtg gtcagcagat gggtgcctca    240

gacgaggaga ttcaggatgt gtcagggacg tggtatctga aggccatgac ggtggacagg    300

gagttccctg agatgaatct ggaatcggtg acacccatga ccctcacgac cctggaaggg    360

ggcaacctgg aagccaaggt caccatgctg ataagtggcc ggagccagga ggtgaaggcc    420

gtcctggaga aaactgacga gccgggaaaa tacacggccg acgggggcaa gcacgtggca    480

tacatcatca ggtcgcacgt gaaggaccac tacatctttt actctgaggg cgagctccac    540

gggaagccgg tcccaggggt gtggctcgtg ggcagagacc ccaagaacaa cctggaagcc    600

ttggaggact ttgagaaagc cgcaggagcc cgcggactca gcacggagag catcctcatc    660

cccaggcaga gcgaaaccag ctctccaggg agcgcttggt ctcacccgca gttcgaaaaa    720

taataagctt gacctgtgaa gtgaaaaatg gcgcacattg tgcgacattt tttttgtctg    780

ccgtttaccg ctactgcgtc acggatctcc acgcgccctg tagcggcgca ttaagcgcgg    840

cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc    900

ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa    960

atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac   1020

ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt   1080

tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca   1140

accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt   1200

taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta   1260

caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct   1320

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat   1380

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg   1440

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg   1500

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc   1560

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat   1620

gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact   1680

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca   1740

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact   1800

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg   1860

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg   1920

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg   1980

aactacttac tctagcttcc cggcaacaat tgatagactg gatggaggcg gataaagttg   2040

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag   2100

ccggtgagcg tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc   2160

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga   2220

tcgctgagat aggtgcctca ctgattaagc attggtagga attaatgatg tctcgtttag   2280

ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt   2340

taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg   2400

taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata   2460

ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa   2520

gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc   2580

ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt   2640

tttcactaga gaatgcatta tatgcactca gcgcagtggg gcattttact ttaggttgcg   2700

tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata   2760

gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag   2820

ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa   2880

gtgggtctta aaagcagcat aacctttttc cgtgatggta acttcactag tttaaaagga   2940

tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt   3000

tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc   3060

tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc   3120

cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac   3180

caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac   3240

cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt   3300

cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct   3360

gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat   3420

acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt   3480

atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg   3540

cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt   3600

gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt   3660

tcctggcctt ttgctggcct tttgctcaca tgacccgaca                         3700



<210>  2
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S191.4-B24 of human tear lipocalin with binding affinity
       for IL-4R alpha

<400>  2

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Ser Arg Cys Pro Arg Ala Tyr Tyr Ser Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Gln Arg Ser Gly Arg Trp Gln Glu Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 


<210>  3
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S351.5-M21 of human tear lipocalin with binding affinity
       for IL-4R alpha

<400>  3

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Ser Arg Cys Pro Arg Ala Tyr Tyr Glu Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Leu Thr Leu Gln Arg Lys Gly Arg Trp Gln Glu Met Lys Asp Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 

<210>  4
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S276.2-K04 of human tear lipocalin with binding affinity
       for IL-4R alpha

<400>  4

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Pro Arg Cys Pro Arg Ala Tyr Tyr Ser Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Gln Arg Ser Gly Arg Trp Gln Lys Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 


<210>  5
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.5-K12 of human tear lipocalin with binding affinity
       for IL-4R alpha

<400>  5

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Leu Arg Cys Pro Arg Ala Tyr Tyr Trp Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Leu Arg Ile Gly Arg Trp Gln Ser Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 


<210>  6
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.5-F08 of human tear lipocalin with binding affinity 
       for IL-4R alpha

<400>  6

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Ser Arg Cys Pro Arg Ala Val Tyr Asn Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Gln Arg Lys Gly Arg Trp Gln Lys Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                 


<210>  7
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.5-L4 of human tear lipocalin with binding affinity for 
       IL-4R alpha

<400>  7

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Ser Arg Cys Pro Arg Ala Tyr Tyr Val Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Ala Arg Ile Gly Arg Trp Gln Ser Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                


<210>  8
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.5-L20 of human tear lipocalin with binding affinity 
       for IL-4R alpha

<400>  8

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Asn Arg Cys Pro Arg Ala Lys Tyr Asp Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala His Arg Arg Gly Arg Trp Gln Gln Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                


<210>  9
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.5-N1 of human tear lipocalin with binding affinity for 
       IL-4R alpha

<400>  9

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Tyr Arg Cys Pro Arg Ala Tyr Tyr His Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala His Arg Ala Gly Arg Trp Gln Lys Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                


<210>  10
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.3-O10 of human tear lipocalin with binding affinity 
       for IL-4R alpha

<400>  10

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Lys Arg Cys Pro Arg Ala Tyr Tyr Arg Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Lys Arg Asn Gly Arg Trp Gln Pro Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                


<210>  11
<211>  154
<212>  PRT
<213>  Artificial

<220>
<223>  Mutein S308.5-N20 of human tear lipocalin with binding affinity 
       for IL-4R alpha

<400>  11

Ala Ser Asp Glu Glu Ile Gln Asp Val Ser Gly Thr Trp Tyr Leu Lys 
1               5                   10                  15      


Ala Met Thr Val Asp Glu Arg Cys Pro Arg Ala His Tyr Gly Ser Val 
            20                  25                  30          


Thr Pro Met Thr Leu Thr Thr Leu Glu Gly Gly Asn Leu Glu Ala Lys 
        35                  40                  45              


Phe Thr Ala Met Arg Leu Gly Arg Trp Gln Lys Tyr Lys Leu Val Leu 
    50                  55                  60                  


Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr Ala Ser Gly Gly Arg His 
65                  70                  75                  80  


Val Ala Tyr Ile Ile Arg Ser His Val Lys Asp His Tyr Ile Phe His 
                85                  90                  95      


Ser Glu Gly Leu Cys Pro Gly Gln Pro Val Pro Gly Val Trp Leu Val 
            100                 105                 110         


Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala Leu Glu Asp Phe Glu Lys 
        115                 120                 125             


Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu Ser Ile Leu Ile Pro Arg 
    130                 135                 140                 


Gln Ser Glu Thr Ser Ser Pro Gly Ser Ala 
145                 150                


<210>  12
<211>  456
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of Mutein S276.2-K04 of human tear lipocalin 
       with binding affinity for IL-4R alpha

<400>  12

gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg      60

gacccccgct gcccgcgggc gtactacagc tcggtgacac ccatgaccct cacgaccctg     120

gaagggggca acctggaagc caagttcacc gcgcagcggt cgggccggtg gcagaagtac     180

aagttggtcc tggagaaaac tgatgagccg ggaaaatata ctgcctccgg gggcaggcac     240

gtggcataca tcatcaggtc gcacgtgaag gaccactaca tctttcactc tgagggcctg     300

tgccccgggc agccggtccc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg     360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc     420

ctcatcccca ggcagagcga aaccagctct ccaggg				          456


<210>  13
<211>  459
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of Mutein S308.5-F08 of human tear lipocalin 
       with binding affinity for IL-4R alpha

<400>  13

gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg      60

gacagtcgct gcccgcgggc ggtgtacaat tcggtgacac ccatgaccct cacgaccctg     120

gaagggggca acctggaagc caagttcacc gctcagcgga agggccggtg gcagaagtac     180

aagttggtcc tggagaaaac tgatgagccg ggaaaataca ctgcctccgg gggcaggcac     240

gtggcataca tcatcaggtc gcacgtgaag gaccactaca tctttcactc tgagggcctg     300

tgccccgggc agccggtccc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg     360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc     420

ctcatcccca ggcagagcga aaccagctct ccagggagc			          459


<210>  14
<211>  456
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of Mutein S308.5-L4 of human tear lipocalin 
       with binding affinity for IL-4R alpha

<400>  14

gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg      60

gactcgcgct gcccgcgggc gtattacgtg tcggtgacac ccatgaccct cacgaccctg     120

gaagggggca acctggaagc caagttcacc gcggcgcgga ttggccggtg gcagagttac     180

aagttggtcc tggagaaaac tgatgagccg ggaaaataca ctgcctccgg gggcaggcac     240

gtggcataca ttatcaggtc gcacgtgaag gaccactaca tctttcactc tgagggcctg     300

tgccccgggc agccggtccc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg     360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc     420

ctcatcccca ggcagagcga aaccagctct ccaggg				          456


<210>  15
<211>  456
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of Mutein S308.5-L20 of human tear lipocalin 
       with binding affinity for IL-4R alpha


<400>  15

gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg      60
gacaatcgct gcccgcgggc gaagtacgat tcggtgacac ccatgaccct cacgaccctg     120

gaagggggca acctggaagc caagttcacc gcgcatcggc ggggccggtg gcagcagtac     180

aagttggtcc tggagaaaac tgatgagccg ggaaaataca ctgcctccgg gggcaggcac     240

gtggcataca tcatcaggtc gcacgtgaag gaccactaca tctttcactc tgagggcctg     300

tgccccgggc agccggtccc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg     360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc     420

ctcatcccca ggcagagcga aaccagctct ccaggg				          456


<210>  16
<211>  456
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of Mutein S308.5-N1 of human tear lipocalin 
       with binding affinity for IL-4R alpha

<400>  16

gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg      60

gactatcgct gcccgcgggc gtattaccat tcggtgacac ccatgaccct cacgaccctg     120

gaagggggca acctggaagc caagttcacc gctcatcggg ctggccggtg gcagaagtac     180

aagttggtcc tggagaaaac tgatgagccg ggaaaataca ctgcctccgg gggcaggcac     240

gtggcataca tcatcaggtc gcacgtgaag gaccactaca tctttcactc tgagggcctg     300

tgccccgggc agccggtccc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg     360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc     420

ctcatcccca ggcagagcga aaccagctct ccaggg				          456


<210>  17
<211>  456
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence of Mutein S308.5-N20 of human tear lipocalin 
       with binding affinity for IL-4R alpha

<400>  17

gcctcagacg aggagattca ggatgtgtca gggacgtggt atctgaaggc catgacggtg      60 

gacgagcgct gcccgcgggc gcattacggg tcggtgacac ccatgaccct cacgaccctg     120 

gaagggggca acctggaagc caagttcacc gctatgcggt tgggccggtg gcagaagtac     180

aagttggtcc tggagaaaac tgatgagccg ggaaaataca ctgcctccgg gggcaggcac     240

gtggcataca tcatcaggtc gcacgtgaag gaccactaca tctttcactc tgagggcctg     300

tgccccgggc agccggtccc aggggtgtgg ctcgtgggca gagaccccaa gaacaacctg     360

gaagccttgg aggactttga gaaagccgca ggagcccgcg gactcagcac ggagagcatc     420

ctcatcccca ggcagagcga aaccagctct ccaggg				          456


<210>  18
<211>  825
<212>  PRT
<213>  Homo sapiens

<220>
<223>  Human IL-4 receptor

<400>  18

Met Gly Trp Leu Cys Ser Gly Leu Leu Phe Pro Val Ser Cys Leu Val 
1               5                   10                  15      


Leu Leu Gln Val Ala Ser Ser Gly Asn Met Lys Val Leu Gln Glu Pro 
            20                  25                  30          


Thr Cys Val Ser Asp Tyr Met Ser Ile Ser Thr Cys Glu Trp Lys Met 
        35                  40                  45              


Asn Gly Pro Thr Asn Cys Ser Thr Glu Leu Arg Leu Leu Tyr Gln Leu 
    50                  55                  60                  


Val Phe Leu Leu Ser Glu Ala His Thr Cys Ile Pro Glu Asn Asn Gly 
65                  70                  75                  80  


Gly Ala Gly Cys Val Cys His Leu Leu Met Asp Asp Val Val Ser Ala 
                85                  90                  95      


Asp Asn Tyr Thr Leu Asp Leu Trp Ala Gly Gln Gln Leu Leu Trp Lys 
            100                 105                 110         


Gly Ser Phe Lys Pro Ser Glu His Val Lys Pro Arg Ala Pro Gly Asn 
        115                 120                 125             


Leu Thr Val His Thr Asn Val Ser Asp Thr Leu Leu Leu Thr Trp Ser 
    130                 135                 140                 


Asn Pro Tyr Pro Pro Asp Asn Tyr Leu Tyr Asn His Leu Thr Tyr Ala 
145                 150                 155                 160 


Val Asn Ile Trp Ser Glu Asn Asp Pro Ala Asp Phe Arg Ile Tyr Asn 
                165                 170                 175     


Val Thr Tyr Leu Glu Pro Ser Leu Arg Ile Ala Ala Ser Thr Leu Lys 
            180                 185                 190         


Ser Gly Ile Ser Tyr Arg Ala Arg Val Arg Ala Trp Ala Gln Cys Tyr 
        195                 200                 205             


Asn Thr Thr Trp Ser Glu Trp Ser Pro Ser Thr Lys Trp His Asn Ser 
    210                 215                 220                 


Tyr Arg Glu Pro Phe Glu Gln His Leu Leu Leu Gly Val Ser Val Ser 
225                 230                 235                 240 


Cys Ile Val Ile Leu Ala Val Cys Leu Leu Cys Tyr Val Ser Ile Thr 
                245                 250                 255     


Lys Ile Lys Lys Glu Trp Trp Asp Gln Ile Pro Asn Pro Ala Arg Ser 
            260                 265                 270         


Arg Leu Val Ala Ile Ile Ile Gln Asp Ala Gln Gly Ser Gln Trp Glu 
        275                 280                 285             


Lys Arg Ser Arg Gly Gln Glu Pro Ala Lys Cys Pro His Trp Lys Asn 
    290                 295                 300                 


Cys Leu Thr Lys Leu Leu Pro Cys Phe Leu Glu His Asn Met Lys Arg 
305                 310                 315                 320 


Asp Glu Asp Pro His Lys Ala Ala Lys Glu Met Pro Phe Gln Gly Ser 
                325                 330                 335     


Gly Lys Ser Ala Trp Cys Pro Val Glu Ile Ser Lys Thr Val Leu Trp 
            340                 345                 350         


Pro Glu Ser Ile Ser Val Val Arg Cys Val Glu Leu Phe Glu Ala Pro 
        355                 360                 365             


Val Glu Cys Glu Glu Glu Glu Glu Val Glu Glu Glu Lys Gly Ser Phe 
    370                 375                 380                 


Cys Ala Ser Pro Glu Ser Ser Arg Asp Asp Phe Gln Glu Gly Arg Glu 
385                 390                 395                 400 


Gly Ile Val Ala Arg Leu Thr Glu Ser Leu Phe Leu Asp Leu Leu Gly 
                405                 410                 415     


Glu Glu Asn Gly Gly Phe Cys Gln Gln Asp Met Gly Glu Ser Cys Leu 
            420                 425                 430         


Leu Pro Pro Ser Gly Ser Thr Ser Ala His Met Pro Trp Asp Glu Phe 
        435                 440                 445             


Pro Ser Ala Gly Pro Lys Glu Ala Pro Pro Trp Gly Lys Glu Gln Pro 
    450                 455                 460                 


Leu His Leu Glu Pro Ser Pro Pro Ala Ser Pro Thr Gln Ser Pro Asp 
465                 470                 475                 480 


Asn Leu Thr Cys Thr Glu Thr Pro Leu Val Ile Ala Gly Asn Pro Ala 
                485                 490                 495     


Tyr Arg Ser Phe Ser Asn Ser Leu Ser Gln Ser Pro Cys Pro Arg Glu 
            500                 505                 510         


Leu Gly Pro Asp Pro Leu Leu Ala Arg His Leu Glu Glu Val Glu Pro 
        515                 520                 525             


Glu Met Pro Cys Val Pro Gln Leu Ser Glu Pro Thr Thr Val Pro Gln 
    530                 535                 540                 


Pro Glu Pro Glu Thr Trp Glu Gln Ile Leu Arg Arg Asn Val Leu Gln 
545                 550                 555                 560 


His Gly Ala Ala Ala Ala Pro Val Ser Ala Pro Thr Ser Gly Tyr Gln 
                565                 570                 575     


Glu Phe Val His Ala Val Glu Gln Gly Gly Thr Gln Ala Ser Ala Val 
            580                 585                 590         


Val Gly Leu Gly Pro Pro Gly Glu Ala Gly Tyr Lys Ala Phe Ser Ser 
        595                 600                 605             


Leu Leu Ala Ser Ser Ala Val Ser Pro Glu Lys Cys Gly Phe Gly Ala 
    610                 615                 620                 


Ser Ser Gly Glu Glu Gly Tyr Lys Pro Phe Gln Asp Leu Ile Pro Gly 
625                 630                 635                 640 


Cys Pro Gly Asp Pro Ala Pro Val Pro Val Pro Leu Phe Thr Phe Gly 
                645                 650                 655     


Leu Asp Arg Glu Pro Pro Arg Ser Pro Gln Ser Ser His Leu Pro Ser 
            660                 665                 670         


Ser Ser Pro Glu His Leu Gly Leu Glu Pro Gly Glu Lys Val Glu Asp 
        675                 680                 685             


Met Pro Lys Pro Pro Leu Pro Gln Glu Gln Ala Thr Asp Pro Leu Val 
    690                 695                 700                 


Asp Ser Leu Gly Ser Gly Ile Val Tyr Ser Ala Leu Thr Cys His Leu 
705                 710                 715                 720 


Cys Gly His Leu Lys Gln Cys His Gly Gln Glu Asp Gly Gly Gln Thr 
                725                 730                 735     


Pro Val Met Ala Ser Pro Cys Cys Gly Cys Cys Cys Gly Asp Arg Ser 
            740                 745                 750         


Ser Pro Pro Thr Thr Pro Leu Arg Ala Pro Asp Pro Ser Pro Gly Gly 
        755                 760                 765             


Val Pro Leu Glu Ala Ser Leu Cys Pro Ala Ser Leu Ala Pro Ser Gly 
    770                 775                 780                 


Ile Ser Glu Lys Ser Lys Ser Ser Ser Ser Phe His Pro Ala Pro Gly 
785                 790                 795                 800 


Asn Ala Gln Ser Ser Ser Gln Thr Pro Lys Ile Val Asn Phe Val Ser 
                805                 810                 815     


Val Gly Pro Thr Tyr Met Arg Val Ser 
            820                 825 


<210>  19
<211>  427
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human IL-13 receptor alpha 1

<400>  19

Met Glu Trp Pro Ala Arg Leu Cys Gly Leu Trp Ala Leu Leu Leu Cys 
1               5                   10                  15      


Ala Gly Gly Gly Gly Gly Gly Gly Gly Ala Ala Pro Thr Glu Thr Gln 
            20                  25                  30          


Pro Pro Val Thr Asn Leu Ser Val Ser Val Glu Asn Leu Cys Thr Val 
        35                  40                  45              


Ile Trp Thr Trp Asn Pro Pro Glu Gly Ala Ser Ser Asn Cys Ser Leu 
    50                  55                  60                  


Trp Tyr Phe Ser His Phe Gly Asp Lys Gln Asp Lys Lys Ile Ala Pro 
65                  70                  75                  80  


Glu Thr Arg Arg Ser Ile Glu Val Pro Leu Asn Glu Arg Ile Cys Leu 
                85                  90                  95      


Gln Val Gly Ser Gln Cys Ser Thr Asn Glu Ser Glu Lys Pro Ser Ile 
            100                 105                 110         


Leu Val Glu Lys Cys Ile Ser Pro Pro Glu Gly Asp Pro Glu Ser Ala 
        115                 120                 125             


Val Thr Glu Leu Gln Cys Ile Trp His Asn Leu Ser Tyr Met Lys Cys 
    130                 135                 140                 


Ser Trp Leu Pro Gly Arg Asn Thr Ser Pro Asp Thr Asn Tyr Thr Leu 
145                 150                 155                 160 


Tyr Tyr Trp His Arg Ser Leu Glu Lys Ile His Gln Cys Glu Asn Ile 
                165                 170                 175     


Phe Arg Glu Gly Gln Tyr Phe Gly Cys Ser Phe Asp Leu Thr Lys Val 
            180                 185                 190         


Lys Asp Ser Ser Phe Glu Gln His Ser Val Gln Ile Met Val Lys Asp 
        195                 200                 205             


Asn Ala Gly Lys Ile Lys Pro Ser Phe Asn Ile Val Pro Leu Thr Ser 
    210                 215                 220                 


Arg Val Lys Pro Asp Pro Pro His Ile Lys Asn Leu Ser Phe His Asn 
225                 230                 235                 240 


Asp Asp Leu Tyr Val Gln Trp Glu Asn Pro Gln Asn Phe Ile Ser Arg 
                245                 250                 255     


Cys Leu Phe Tyr Glu Val Glu Val Asn Asn Ser Gln Thr Glu Thr His 
            260                 265                 270         


Asn Val Phe Tyr Val Gln Glu Ala Lys Cys Glu Asn Pro Glu Phe Glu 
        275                 280                 285             


Arg Asn Val Glu Asn Thr Ser Cys Phe Met Val Pro Gly Val Leu Pro 
    290                 295                 300                 


Asp Thr Leu Asn Thr Val Arg Ile Arg Val Lys Thr Asn Lys Leu Cys 
305                 310                 315                 320 


Tyr Glu Asp Asp Lys Leu Trp Ser Asn Trp Ser Gln Glu Met Ser Ile 
                325                 330                 335     


Gly Lys Lys Arg Asn Ser Thr Leu Tyr Ile Thr Met Leu Leu Ile Val 
            340                 345                 350         


Pro Val Ile Val Ala Gly Ala Ile Ile Val Leu Leu Leu Tyr Leu Lys 
        355                 360                 365             


Arg Leu Lys Ile Ile Ile Phe Pro Pro Ile Pro Asp Pro Gly Lys Ile 
    370                 375                 380                 


Phe Lys Glu Met Phe Gly Asp Gln Asn Asp Asp Thr Leu His Trp Lys 
385                 390                 395                 400 


Lys Tyr Asp Ile Tyr Glu Lys Gln Thr Lys Glu Glu Thr Asp Ser Val 
                405                 410                 415     


Val Leu Ile Glu Asn Leu Lys Lys Ala Ser Gln 
            420                 425         


<210>  20
<211>  176
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human tear lipocalin (Tlc)

<400>  20

Met Lys Pro Leu Leu Leu Ala Val Ser Leu Gly Leu Ile Ala Ala Leu 
1               5                   10                  15      


Gln Ala His His Leu Leu Ala Ser Asp Glu Glu Ile Gln Asp Val Ser 
            20                  25                  30          


Gly Thr Trp Tyr Leu Lys Ala Met Thr Val Asp Arg Glu Phe Pro Glu 
        35                  40                  45              


Met Asn Leu Glu Ser Val Thr Pro Met Thr Leu Thr Thr Leu Glu Gly 
    50                  55                  60                  


Gly Asn Leu Glu Ala Lys Val Thr Met Leu Ile Ser Gly Arg Cys Gln 
65                  70                  75                  80  


Glu Val Lys Ala Val Leu Glu Lys Thr Asp Glu Pro Gly Lys Tyr Thr 
                85                  90                  95      


Ala Asp Gly Gly Lys His Val Ala Tyr Ile Ile Arg Ser His Val Lys 
            100                 105                 110         


Asp His Tyr Ile Phe Tyr Cys Glu Gly Glu Leu His Gly Lys Pro Val 
        115                 120                 125             


Arg Gly Val Lys Leu Val Gly Arg Asp Pro Lys Asn Asn Leu Glu Ala 
    130                 135                 140                 


Leu Glu Asp Phe Glu Lys Ala Ala Gly Ala Arg Gly Leu Ser Thr Glu 
145                 150                 155                 160 


Ser Ile Leu Ile Pro Arg Gln Ser Glu Thr Cys Ser Pro Gly Ser Asp 
                165                 170                 175     


1


