                         SEQUENCE LISTING

<110>  DANA-FARBER CANCER INSTITUTE, INC
       Sui, Jianhua
       Avnir, Yuval
       Marasco, Wayne
 
<120>  Humanized Monoclonal Antibodies and Methods of Use

<130>  20363-057001WO

<140>  PCT/US2011/038970
<141>  2011-06-02

<150>  61/350,790
<151>  2010-06-02

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  354
<212>  DNA
<213>  Homo sapiens

<400>  1
caggtccagc tcgtccagtc cggcgctgaa gtggtgaaac ccggggcatc cgtcaaagtc       60

tcttgtaagg ctagtggcta caccttcaca tcctactgga tgcattgggt gaaacaggca      120

cctggccagg gactcgaatg gatcggagcc gtgtctcctg gaaattccga cacctcctac      180

aacgaaaaat tcaagggcaa ggcaaccctc actgtggata ctagtgcttc taccgcctac      240

atggaactct catctctccg ctctgaggac actgccgtct actactgtac tcggtcacga      300

tacgggaaca acgctctcga ttactgggga cagggcacac tggtcactgt ctct            354


<210>  2
<211>  118
<212>  PRT
<213>  Homo sapiens

<400>  2

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Val Ser Pro Gly Asn Ser Asp Thr Ser Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Arg Tyr Gly Asn Asn Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser 
        115             


<210>  3
<211>  321
<212>  DNA
<213>  Homo sapiens

<400>  3
gatattcagc tcacacagag cccatcttct ctgtctgctt ctgtgggcga tcgagtgaca       60

atcacttgtc gggctagtca gggcatttct agcaacattg tgtggctcca gcagaaacct      120

ggcaaagccc caaaaggcct catctaccac ggaaccaacc tggaatctgg cgtgccatct      180

cggtttagtg gatctggatc cgggaccgat tacacactca ccatctcttc actggaacct      240

gaggatttcg ccacctacta ctgtgtccag tactcccagt ttccacccac ttttggacag      300

ggaaccaaac tcgagatcaa a                                                321


<210>  4
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  4

Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Asn 
            20                  25                  30          


Ile Val Trp Leu Gln Gln Lys Pro Gly Lys Ala Pro Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ser Gln Phe Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  5
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  5

Gly Tyr Thr Phe Thr Ser Tyr Trp 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  6

Val Ser Pro Gly Asn Ser Asp Thr 
1               5               


<210>  7
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  7

Thr Arg Ser Arg Tyr Gly Asn Asn Ala Leu Asp Tyr 
1               5                   10          


<210>  8
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  8

Gln Gly Ile Ser Ser Asn Ile Val Trp 
1               5                   


<210>  9
<211>  3
<212>  PRT
<213>  Homo sapiens

<400>  9

His Gly Thr 
1           


<210>  10
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  10

Val Gln Tyr Ser Gln Phe Pro Pro Thr 
1               5                   


<210>  11
<211>  354
<212>  DNA
<213>  Homo sapiens

<400>  11
caggtccagc tcgtccagtc cggcgctgaa gtggtgaaac ccggggcatc cgtcaaagtc       60

tcttgtaagg ctagtggcta caccttcaca tcctactgga tgcattgggt gaaacaggca      120

cctggccagg gactcgaatg gatcggagcc gtgtctcctg gaaattccga cacctcctac      180

aacgaaaaat tcaagggcaa ggcaaccctc actgtggaca aatctgcctc taccgcctac      240

atggaactct catctctccg ctctgaggat actgctgtgt actactgtac ccggtcacga      300

tacggcaata acgccctcga ttactggggg cagggaactc tggtcactgt gtct            354


<210>  12
<211>  118
<212>  PRT
<213>  Homo sapiens

<400>  12

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Val Ser Pro Gly Asn Ser Asp Thr Ser Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Arg Tyr Gly Asn Asn Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser 
        115             


<210>  13
<211>  357
<212>  DNA
<213>  Mus musculus

<400>  13
caggtccagc tgcagcagtc tgggactgtg ctcgcaaggc ctggggcttc agtgaagatg       60

tcctgcaagg cttctggcta cacctttacc agttactgga tgcactgggt aaaacagagg      120

cctggacagg gtctggaatg gattggcgct gtttctcctg gaaatagtga tactagctac      180

aaccagaagt tcaagggcaa ggccacactg actgcagtca catccaccag cactgcctac      240

atggagttca gcagcctgac aaatgaggac tctgcggtct attactgtac aagaagtcga      300

tatggtaaca atgctttgga ctactggggc caagggacca cggtcaccgt ctcctca         357


<210>  14
<211>  119
<212>  PRT
<213>  Mus musculus

<400>  14

Gln Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Val Ser Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Val Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Phe Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Arg Tyr Gly Asn Asn Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  15
<211>  330
<212>  DNA
<213>  Mus musculus

<400>  15
gacatcgagc tcacccagtc tcctgcttcc ttagctgtat ctctggggca gagggccacc       60

atctcataca gggccagcaa aagtgtcagt acatctggct atagttatat gcactggaac      120

caacagaaac caggacagcc acccagactc ctcatctatc ttgtatccaa cctagaatct      180

ggggtccctg ccaggttcag tggcagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatgc tgcaacctat tactgtcagc acattaaggg agcttacacg      300

ttcggagggg ggaccaagct ggaaataaaa                                       330


<210>  16
<211>  330
<212>  DNA
<213>  Mus musculus

<400>  16
gacatcgagc tcactcagtc tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca gagccagcgc aagtgttgat aattatggca ttagttttat gaactggttc      120

caacagaaac caggacagcc acccaaactc ctcatctatg ctgcatccaa ccaaggatcc      180

ggggtccctg ccaggtttag tggcagtggg tctgggacag acttcagcct caacatccat      240

cctatggagg aggatgatac tgcaacctat tactgtcagc acattaaggg agcttacacg      300

ttcggagggg ggaccaagct ggagctgaaa                                       330


<210>  17
<211>  321
<212>  DNA
<213>  Mus musculus

<400>  17
gacatcgagc tcactcagtc tccatcctcc atgtctgtat ctctgggaga cacagtcaac       60

atcacttgcc gtgcaagtca gggcattagc agtaatatag tgtggttgca gcagaaacca      120

gggaagtcat ttaagggcct gatctatcat gggaccaatt tggaagatgg agttccatca      180

aggttcagtg gcagtggatc tggagccgat tattctctca ccatcagcag cctggaatct      240

gaggattttg cagactatta ctgtgtacag tattctcagt ttcctcccac gttcggctcg      300

gggaccaagc tggagctgaa a                                                321


<210>  18
<211>  107
<212>  PRT
<213>  Mus musculus

<400>  18

Asp Ile Glu Leu Thr Gln Ser Pro Ser Ser Met Ser Val Ser Leu Gly 
1               5                   10                  15      


Asp Thr Val Asn Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Asn 
            20                  25                  30          


Ile Val Trp Leu Gln Gln Lys Pro Gly Lys Ser Phe Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Glu Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Ala Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Val Gln Tyr Ser Gln Phe Pro Pro 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Leu Lys 
            100                 105         


<210>  19
<211>  118
<212>  PRT
<213>  Homo sapiens

<400>  19

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Val Ser Pro Gly Asn Ser Asp Thr Ser Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Arg Tyr Gly Asn Asn Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser 
        115             


<210>  20
<211>  118
<212>  PRT
<213>  Mus musculus

<400>  20

Gln Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Val Ser Pro Gly Asn Ser Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Val Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Phe Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser Arg Tyr Gly Asn Asn Ala Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser 
        115             


<210>  21
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  21

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Asn 
            20                  25                  30          


Ile Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Gly Leu Ile 
        35                  40                  45              


Tyr His Gly Thr Asn Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Val Gln Tyr Ser Gln Phe Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  22
<211>  98
<212>  PRT
<213>  Homo sapiens

<400>  22

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg 
        


<210>  23
<211>  294
<212>  DNA
<213>  Homo sapiens

<400>  23
caggtgcagc tggtgcagtc tggggctgag gtgaagaagc ctgggtcctc ggtgaaggtc       60

tcctgcaagg cttctggagg caccttcagc agctatgcta tcagctgggt gcgacaggcc      120

cctggacaag ggcttgagtg gatgggaggg atcatcccta tctttggtac agcaaactac      180

gcacagaagt tccagggcag agtcacgatt accgcggacg aatccacgag cacagcctac      240

atggagctga gcagcctgag atctgaggac acggccgtgt attactgtgc gaga            294


<210>  24
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized peptide

<400>  24

Ile Ser Pro Met Phe Gly Thr Pro 
1               5               


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized peptide

<400>  25

Ile Ile Pro Ile Phe Gly Thr Ala 
1               5               


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized peptide

<400>  26

Ile Ser Pro Ile Phe Gly Thr Ala 
1               5               


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  wherein said Xaa is an Isoleucine or a Serine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  wherein said Xaa is an Isoleucine or a Methionine

<400>  27

Ile Xaa Pro Xaa Phe Gly Thr Ala 
1               5               


<210>  28
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  wherein said Xaa is a Glycine or a Glutamic acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  wherein said Xaa is a Glycine or a Valine

<400>  28

Xaa Xaa Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  29
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  wherein said Xaa is an Isoleucine or a Serine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  wherein said Xaa is an Isoleucine or a Methionine

<400>  29

Ile Xaa Pro Xaa Phe Gly Thr Ala 
1               5               


