                         SEQUENCE LISTING

<110>  INSERM
 
<120>  Constitutively active prolactin receptor variants as prognostic 
       markers and therapeutic targets to prevent progression of 
       hormone-dependent cancer towards hormone independence

<130>  BET 11P1489

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  1000
<212>  DNA
<213>  Homo sapiens

<400>  1
ggaggctgaa atccccagac gccggttttc tgggctgggc tttctgctta ctcactcctt       60

ctccctcttt ctggatttta ccgaccgttc gcgaaacagc tttccacaca atggagcttc      120

atgtcctcgt gcaggaagta ctcatcgact gatgtggcag actttgctcc ctgacaaaac      180

taaagaactc tcctattcat ggaggcgaac actgaggatg ctttccacat gaaccctgaa      240

gtgaacttct gatacatttc ctgcagcaag agaaggcagc caacatgaag gaaaatgtgg      300

catctgcaac cgttttcact ctgctacttt ttctcaacac ctgccttctg aatggacagt      360

tacctcctgg aaaacctgag atctttaaat gtcgttctcc caataaggaa acattcacct      420

gctggtggag gcctgggaca gatggaggac ttcctaccaa ttattcactg acttaccaca      480

gggaaggaga gacactcatg catgaatgtc cagactacat aaccggtggc cccaactcct      540

gccactttgg caagcagtac acctccatgt ggaggacata catcatgatg gtcaatgcca      600

ctaaccagat gggaagcagt ttctcggatg aactttatgt ggacgtgact tacatagttc      660

agccagaccc tcctttggag ctggctgtgg aagtaaaaca gccagaagac agaaaaccct      720

acctgtggat taaatggtct ccacctaccc tgattgactt aaaaactggt tggttcacgc      780

tcctgtatga aattcgatta aaacccgaga aagcagctga gtgggagatc cattttgctg      840

ggcagcaaac agagtttaag attctcagcc tacatccagg acagaaatac cttgtccagg      900

ttcgctgcaa accagaccat ggatactgga gtgcatggag tccagcgacc ttcattcaga      960

tacctagtga cttcaccatg aatgatacaa ccgtgtggat                           1000


<210>  2
<211>  622
<212>  PRT
<213>  Homo sapiens


<220>
<221>  VARIANT
<222>  (100)..(100)
<223>  Ile to Val substitution

<220>
<221>  VARIANT
<222>  (170)..(170)
<223>  Ile to Leu substitution

<400>  2

Met Lys Glu Asn Val Ala Ser Ala Thr Val Phe Thr Leu Leu Leu Phe 
1               5                   10                  15      


Leu Asn Thr Cys Leu Leu Asn Gly Gln Leu Pro Pro Gly Lys Pro Glu 
            20                  25                  30          


Ile Phe Lys Cys Arg Ser Pro Asn Lys Glu Thr Phe Thr Cys Trp Trp 
        35                  40                  45              


Arg Pro Gly Thr Asp Gly Gly Leu Pro Thr Asn Tyr Ser Leu Thr Tyr 
    50                  55                  60                  


His Arg Glu Gly Glu Thr Leu Met His Glu Cys Pro Asp Tyr Ile Thr 
65                  70                  75                  80  


Gly Gly Pro Asn Ser Cys His Phe Gly Lys Gln Tyr Thr Ser Met Trp 
                85                  90                  95      


Arg Thr Tyr Ile Met Met Val Asn Ala Thr Asn Gln Met Gly Ser Ser 
            100                 105                 110         


Phe Ser Asp Glu Leu Tyr Val Asp Val Thr Tyr Ile Val Gln Pro Asp 
        115                 120                 125             


Pro Pro Leu Glu Leu Ala Val Glu Val Lys Gln Pro Glu Asp Arg Lys 
    130                 135                 140                 


Pro Tyr Leu Trp Ile Lys Trp Ser Pro Pro Thr Leu Ile Asp Leu Lys 
145                 150                 155                 160 


Thr Gly Trp Phe Thr Leu Leu Tyr Glu Ile Arg Leu Lys Pro Glu Lys 
                165                 170                 175     


Ala Ala Glu Trp Glu Ile His Phe Ala Gly Gln Gln Thr Glu Phe Lys 
            180                 185                 190         


Ile Leu Ser Leu His Pro Gly Gln Lys Tyr Leu Val Gln Val Arg Cys 
        195                 200                 205             


Lys Pro Asp His Gly Tyr Trp Ser Ala Trp Ser Pro Ala Thr Phe Ile 
    210                 215                 220                 


Gln Ile Pro Ser Asp Phe Thr Met Asn Asp Thr Thr Val Trp Ile Ser 
225                 230                 235                 240 


Val Ala Val Leu Ser Ala Val Ile Cys Leu Ile Ile Val Trp Ala Val 
                245                 250                 255     


Ala Leu Lys Gly Tyr Ser Met Val Thr Cys Ile Phe Pro Pro Val Pro 
            260                 265                 270         


Gly Pro Lys Ile Lys Gly Phe Asp Ala His Leu Leu Glu Lys Gly Lys 
        275                 280                 285             


Ser Glu Glu Leu Leu Ser Ala Leu Gly Cys Gln Asp Phe Pro Pro Thr 
    290                 295                 300                 


Ser Asp Tyr Glu Asp Leu Leu Val Glu Tyr Leu Glu Val Asp Asp Ser 
305                 310                 315                 320 


Glu Asp Gln His Leu Met Ser Val His Ser Lys Glu His Pro Ser Gln 
                325                 330                 335     


Gly Met Lys Pro Thr Tyr Leu Asp Pro Asp Thr Asp Ser Gly Arg Gly 
            340                 345                 350         


Ser Cys Asp Ser Pro Ser Leu Leu Ser Glu Lys Cys Glu Glu Pro Gln 
        355                 360                 365             


Ala Asn Pro Ser Thr Phe Tyr Asp Pro Glu Val Ile Glu Lys Pro Glu 
    370                 375                 380                 


Asn Pro Glu Thr Thr His Thr Trp Asp Pro Gln Cys Ile Ser Met Glu 
385                 390                 395                 400 


Gly Lys Ile Pro Tyr Phe His Ala Gly Gly Ser Lys Cys Ser Thr Trp 
                405                 410                 415     


Pro Leu Pro Gln Pro Ser Gln His Asn Pro Arg Ser Ser Tyr His Asn 
            420                 425                 430         


Ile Thr Asp Val Cys Glu Leu Ala Val Gly Pro Ala Gly Ala Pro Ala 
        435                 440                 445             


Thr Leu Leu Asn Glu Ala Gly Lys Asp Ala Leu Lys Ser Ser Gln Thr 
    450                 455                 460                 


Ile Lys Ser Arg Glu Glu Gly Lys Ala Thr Gln Gln Arg Glu Val Glu 
465                 470                 475                 480 


Ser Phe His Ser Glu Thr Asp Gln Asp Thr Pro Trp Leu Leu Pro Gln 
                485                 490                 495     


Glu Lys Thr Pro Phe Gly Ser Ala Lys Pro Leu Asp Tyr Val Glu Ile 
            500                 505                 510         


His Lys Val Asn Lys Asp Gly Ala Leu Ser Leu Leu Pro Lys Gln Arg 
        515                 520                 525             


Glu Asn Ser Gly Lys Pro Lys Lys Pro Gly Thr Pro Glu Asn Asn Lys 
    530                 535                 540                 


Glu Tyr Ala Lys Val Ser Gly Val Met Asp Asn Asn Ile Leu Val Leu 
545                 550                 555                 560 


Val Pro Asp Pro His Ala Lys Asn Val Ala Cys Phe Glu Glu Ser Ala 
                565                 570                 575     


Lys Glu Ala Pro Pro Ser Leu Glu Gln Asn Gln Ala Glu Lys Ala Leu 
            580                 585                 590         


Ala Asn Phe Thr Ala Thr Ser Ser Lys Cys Arg Leu Gln Leu Gly Gly 
        595                 600                 605             


Leu Asp Tyr Leu Asp Pro Ala Cys Phe Thr His Ser Phe His 
    610                 615                 620         


<210>  3
<211>  598
<212>  PRT
<213>  Homo sapiens


<220>
<221>  VARIANT
<222>  (76)..(76)
<223>  Ile to Val substitution

<220>
<221>  VARIANT
<222>  (146)..(146)
<223>  Ile to Leu substitution

<400>  3

Gln Leu Pro Pro Gly Lys Pro Glu Ile Phe Lys Cys Arg Ser Pro Asn 
1               5                   10                  15      


Lys Glu Thr Phe Thr Cys Trp Trp Arg Pro Gly Thr Asp Gly Gly Leu 
            20                  25                  30          


Pro Thr Asn Tyr Ser Leu Thr Tyr His Arg Glu Gly Glu Thr Leu Met 
        35                  40                  45              


His Glu Cys Pro Asp Tyr Ile Thr Gly Gly Pro Asn Ser Cys His Phe 
    50                  55                  60                  


Gly Lys Gln Tyr Thr Ser Met Trp Arg Thr Tyr Ile Met Met Val Asn 
65                  70                  75                  80  


Ala Thr Asn Gln Met Gly Ser Ser Phe Ser Asp Glu Leu Tyr Val Asp 
                85                  90                  95      


Val Thr Tyr Ile Val Gln Pro Asp Pro Pro Leu Glu Leu Ala Val Glu 
            100                 105                 110         


Val Lys Gln Pro Glu Asp Arg Lys Pro Tyr Leu Trp Ile Lys Trp Ser 
        115                 120                 125             


Pro Pro Thr Leu Ile Asp Leu Lys Thr Gly Trp Phe Thr Leu Leu Tyr 
    130                 135                 140                 


Glu Ile Arg Leu Lys Pro Glu Lys Ala Ala Glu Trp Glu Ile His Phe 
145                 150                 155                 160 


Ala Gly Gln Gln Thr Glu Phe Lys Ile Leu Ser Leu His Pro Gly Gln 
                165                 170                 175     


Lys Tyr Leu Val Gln Val Arg Cys Lys Pro Asp His Gly Tyr Trp Ser 
            180                 185                 190         


Ala Trp Ser Pro Ala Thr Phe Ile Gln Ile Pro Ser Asp Phe Thr Met 
        195                 200                 205             


Asn Asp Thr Thr Val Trp Ile Ser Val Ala Val Leu Ser Ala Val Ile 
    210                 215                 220                 


Cys Leu Ile Ile Val Trp Ala Val Ala Leu Lys Gly Tyr Ser Met Val 
225                 230                 235                 240 


Thr Cys Ile Phe Pro Pro Val Pro Gly Pro Lys Ile Lys Gly Phe Asp 
                245                 250                 255     


Ala His Leu Leu Glu Lys Gly Lys Ser Glu Glu Leu Leu Ser Ala Leu 
            260                 265                 270         


Gly Cys Gln Asp Phe Pro Pro Thr Ser Asp Tyr Glu Asp Leu Leu Val 
        275                 280                 285             


Glu Tyr Leu Glu Val Asp Asp Ser Glu Asp Gln His Leu Met Ser Val 
    290                 295                 300                 


His Ser Lys Glu His Pro Ser Gln Gly Met Lys Pro Thr Tyr Leu Asp 
305                 310                 315                 320 


Pro Asp Thr Asp Ser Gly Arg Gly Ser Cys Asp Ser Pro Ser Leu Leu 
                325                 330                 335     


Ser Glu Lys Cys Glu Glu Pro Gln Ala Asn Pro Ser Thr Phe Tyr Asp 
            340                 345                 350         


Pro Glu Val Ile Glu Lys Pro Glu Asn Pro Glu Thr Thr His Thr Trp 
        355                 360                 365             


Asp Pro Gln Cys Ile Ser Met Glu Gly Lys Ile Pro Tyr Phe His Ala 
    370                 375                 380                 


Gly Gly Ser Lys Cys Ser Thr Trp Pro Arg Pro Gln Pro Ser Gln His 
385                 390                 395                 400 


Asn Pro Arg Ser Ser Tyr His Asn Ile Thr Asp Val Cys Glu Leu Ala 
                405                 410                 415     


Val Gly Pro Ala Gly Ala Pro Ala Thr Leu Leu Asn Glu Ala Gly Lys 
            420                 425                 430         


Asp Ala Leu Lys Ser Ser Gln Thr Ile Lys Ser Arg Glu Glu Gly Lys 
        435                 440                 445             


Ala Thr Gln Gln Arg Glu Val Glu Ser Phe His Ser Glu Thr Asp Gln 
    450                 455                 460                 


Asp Thr Pro Trp Leu Leu Pro Gln Glu Lys Thr Pro Phe Gly Ser Ala 
465                 470                 475                 480 


Lys Pro Leu Asp Tyr Val Glu Ile His Lys Val Asn Lys Asp Gly Ala 
                485                 490                 495     


Leu Ser Leu Leu Pro Lys Gln Arg Glu Asn Ser Gly Lys Pro Lys Lys 
            500                 505                 510         


Pro Gly Thr Pro Glu Asn Asn Lys Glu Tyr Ala Lys Val Ser Gly Val 
        515                 520                 525             


Met Asp Asn Asn Ile Leu Val Leu Val Pro Asp Pro His Ala Lys Asn 
    530                 535                 540                 


Val Ala Cys Phe Glu Glu Ser Ala Lys Glu Ala Pro Pro Ser Leu Glu 
545                 550                 555                 560 


Gln Asn Gln Ala Glu Lys Ala Leu Ala Asn Phe Thr Ala Thr Ser Ser 
                565                 570                 575     


Lys Cys Arg Leu Gln Leu Gly Gly Leu Asp Tyr Leu Asp Pro Ala Cys 
            580                 585                 590         


Phe Thr His Ser Phe His 
        595             


<210>  4
<211>  227
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SIGNAL
<222>  (1)..(28)

<220>
<221>  mat_peptide
<222>  (29)..(227)

<400>  4

Met Asn Ile Lys Gly Ser Pro Trp Lys Gly Ser Leu Leu Leu Leu Leu 
            -25                 -20                 -15         


Val Ser Asn Leu Leu Leu Cys Gln Ser Val Ala Pro Leu Pro Ile Cys 
        -10                 -5              -1  1               


Pro Gly Gly Ala Ala Arg Cys Gln Val Thr Leu Arg Asp Leu Phe Asp 
5                   10                  15                  20  


Arg Ala Val Val Leu Ser His Tyr Ile His Asn Leu Ser Ser Glu Met 
                25                  30                  35      


Phe Ser Glu Phe Asp Lys Arg Tyr Thr His Gly Arg Gly Phe Ile Thr 
            40                  45                  50          


Lys Ala Ile Asn Ser Cys His Thr Ser Ser Leu Ala Thr Pro Glu Asp 
        55                  60                  65              


Lys Glu Gln Ala Gln Gln Met Asn Gln Lys Asp Phe Leu Ser Leu Ile 
    70                  75                  80                  


Val Ser Ile Leu Arg Ser Trp Asn Glu Pro Leu Tyr His Leu Val Thr 
85                  90                  95                  100 


Glu Val Arg Gly Met Gln Glu Ala Pro Glu Ala Ile Leu Ser Lys Ala 
                105                 110                 115     


Val Glu Ile Glu Glu Gln Thr Lys Arg Leu Leu Glu Gly Met Glu Leu 
            120                 125                 130         


Ile Val Ser Gln Val His Pro Glu Thr Lys Glu Asn Glu Ile Tyr Pro 
        135                 140                 145             


Val Trp Ser Gly Leu Pro Ser Leu Gln Met Ala Asp Glu Glu Ser Arg 
    150                 155                 160                 


Leu Ser Ala Tyr Tyr Asn Leu Leu His Cys Leu Arg Arg Asp Ser His 
165                 170                 175                 180 


Lys Ile Asp Asn Tyr Leu Lys Leu Leu Lys Cys Arg Ile Ile His Asn 
                185                 190                 195     


Asn Asn Cys 
            


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  5
aaattgagcc tgaagccaac                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
gtggccactg tgcttactga                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
cctgggactc cacctacaga                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  8
tgtgagcaat tctgcttgga                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
ggagacaggc tggttgttgt                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
ccactgaggc accattctct                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  11
cacagtctgc tgaggttgga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
caagctggcc ttcagatttc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
actacacgac catccaggac                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
tctcaaactt ggttcggaag                                                   20


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
tgcctttttg aagaaactcc a                                                 21


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
gtgaggtcag gcttggaaac                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  17
gccaccatct acctcctcaa                                                   20


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
cagacagaag gcttttcatc g                                                 21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
tccaatggca agtttgtgac                                                   20


<210>  20
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
gatgatgggg cggttgat                                                     18


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
gcgaccctaa acacctcaac                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  22
acagcaagcg gtaaaaccag                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  23
ggctcaactc gcatctcaag                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
tgatttcaaa gagggcatgg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
gtccgcagtc ttacgaggag                                                   20


<210>  26
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
gcagcgtggg gatgatct                                                     18


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
ctctttcctc gtcaggaagc                                                   20


<210>  28
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
gtcgtagggc tgctggaag                                                    19


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
atttcaacgc ctccaaaaa                                                    19


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
ttgtggtatg acaggcatgg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
tccttacctg tgggagctgt                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
gcggatttta tcaacgatgc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
tggagtctgg tcctgtgagg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
ggcagctctt cctcctgttt                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
tgtgactgcc tgtccctaca                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
gcttgctgca cttctcacac                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
ctgggacaga tggaggactt                                                   20


<210>  38
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
gggccaccgg ttatgtagt                                                    19


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
caggtcctgg catcttgtcc                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
ttgctggtct tgccattcct                                                   20


