                         SEQUENCE LISTING

<110>  Dr. Reddys Laboratories Limited, Dr. Reddy's Laboratories, Inc.
 
<120>  Preparation of (3aS,7aR)-hexahydroisobenzofuran-1(3H)-one through
        the use of NovozymTM 435 catalysed biological resolution of 
       dimethyl cyclohexane-1,2-dicarboxylate

<130>  CPS 3.4-020

<160>  3     

<170>  PatentIn version 3.3

<210>  1
<211>  444
<212>  PRT
<213>  Ophiostoma

<400>  Seq Id No 1: Esterase K

Met Thr Met Ile Thr Pro Ser Ser Ala Met Gly Met Ser Ser Thr Phe 
1               5                   10                  15      


Glu Phe Glu Arg Val Ile Thr Lys Ala Val Glu Asp Arg Val Ile Pro 
            20                  25                  30          


Gly Val Val Leu Leu Ala Glu Asn Ser Ser Gly Ser Tyr His Tyr Glu 
        35                  40                  45              


Lys Val Leu Gly Tyr Ser Ser Ile Glu Ala Gly Asn Glu Lys Lys Leu 
    50                  55                  60                  


Glu Arg Asp Ser Val Phe Thr Phe Met Ser Met Thr Lys Phe Ile Thr 
65                  70                  75                  80  


Ala Ile Val Ala Met Gln Ala Val Glu Arg Gly Leu Trp Asp Leu Asp 
                85                  90                  95      


Ala Asp Val Ala Pro Leu Leu Pro Glu Leu Ala Ala Leu Pro Val Leu 
            100                 105                 110         


Lys Gly Phe Ser Asp Asp Gly Val Pro Glu Leu Val Pro Arg Glu Ser 
        115                 120                 125             


Ala Ile Thr Leu Arg Gln Leu Leu Ser His Thr Ser Gly Ala Ala Tyr 
    130                 135                 140                 


Asp Phe Leu Ser Pro Asp Leu Ile Asn Tyr His Ala Trp Val Arg Lys 
145                 150                 155                 160 


Gln Pro Pro Ser Ala Gly Leu Glu Gln Pro Pro Ala Met Thr Val Ala 
                165                 170                 175     


Pro Pro Ser Val Glu Glu Arg Phe Arg Phe Pro Leu Val Phe Gln Pro 
            180                 185                 190         


Gly Gln Gly Trp Gln Tyr Gly Ser Ser Leu Asp Trp Val Gly Arg Leu 
        195                 200                 205             


Val Glu Arg Leu Asp Ala Lys Thr Gln Gly Lys Thr Glu Lys Glu Ala 
    210                 215                 220                 


Gly Thr Lys Leu Pro Ser Val Pro Leu Glu Glu Ile Val Ile Arg Asp 
225                 230                 235                 240 


Val Leu Thr Pro Leu Gly Leu Pro Ala Gly Ala Leu Thr Phe Ser Pro 
                245                 250                 255     


Glu Arg Tyr Pro Asp Val Phe Ala Arg Met Trp Pro Ser Leu Pro Val 
            260                 265                 270         


Arg Val Gly Asn Asn Gly Ala Leu Asp Gly Gly Pro Val Val His Gly 
        275                 280                 285             


Pro Ser Val Tyr Lys Lys Ala Pro Ala Ala Leu Gly Gly Gln Gly Met 
    290                 295                 300                 


Tyr Gly Asp Met Pro Ser Phe Phe Lys Val Ala Leu Ser Ile Phe Arg 
305                 310                 315                 320 


Asp Asp Gly Lys Leu Leu Lys Pro Glu Ser Thr Lys Leu Phe Phe Glu 
                325                 330                 335     


Pro Gln Leu Ala Ser Glu Ala Ala His Ala Gly Ile Met His Gly Thr 
            340                 345                 350         


Glu Asn Ser Gly Trp Ile Thr Gly Asp Val Pro Asp Thr Lys Glu Tyr 
        355                 360                 365             


Asp Trp Ser Val Ala Gly Leu Leu Val Thr Gly Asp Ser His Pro Phe 
    370                 375                 380                 


Arg Lys Arg Gly Ala Val Leu Trp Ala Gly Ala Ile Asn Leu Thr Trp 
385                 390                 395                 400 


Ile Ile Asp Lys Glu Ala Asp Val Cys Ala Val Phe Gly Ser Asn Tyr 
                405                 410                 415     


Gln Pro Pro Gly Asp Gln Gln Gly Lys Ala Leu Met Arg Gln Trp Glu 
            420                 425                 430         


Glu Phe Val Tyr Pro Gln Ala Lys Thr Ala Lys Leu 
        435                 440                 


<210>  2
<211>  444
<212>  PRT
<213>  Ophistoma

<400>  SEQ ID No 2. Esterase N

Met Thr Met Ile Thr Pro Ser Ser Ala Met Gly Met Ser Ser Thr Phe 
1               5                   10                  15      


Glu Phe Glu Arg Val Ile Thr Lys Ala Val Glu Asp Arg Val Ile Pro 
            20                  25                  30          


Gly Val Val Leu Leu Ala Glu Asn Ser Ser Gly Ser Tyr His Tyr Glu 
        35                  40                  45              


Lys Val Leu Gly Tyr Ser Ser Ile Glu Ala Gly Asn Glu Lys Lys Leu 
    50                  55                  60                  


Glu Arg Asp Ser Val Phe Thr Phe Met Ser Met Thr Lys Phe Ile Thr 
65                  70                  75                  80  


Ala Ile Val Ala Met Gln Ala Val Glu Arg Gly Leu Trp Asp Leu Asp 
                85                  90                  95      


Ala Asp Val Ala Pro Leu Leu Pro Glu Leu Ala Ala Leu Pro Val Leu 
            100                 105                 110         


Lys Gly Phe Ser Asp Asp Gly Val Pro Glu Leu Val Pro Arg Glu Ser 
        115                 120                 125             


Ala Ile Thr Leu Arg Gln Leu Leu Ser His Thr Ser Gly Ala Ala Tyr 
    130                 135                 140                 


Asp Phe Leu Ser Pro Asp Leu Ile Asn Tyr His Ala Trp Val Arg Lys 
145                 150                 155                 160 


Gln Pro Pro Ser Ala Gly Leu Glu Gln Pro Pro Ala Met Thr Val Ala 
                165                 170                 175     


Pro Pro Ser Val Glu Glu Arg Phe Arg Phe Pro Leu Val Phe Gln Pro 
            180                 185                 190         


Gly Gln Gly Trp Gln Tyr Gly Ser Ser Leu Asp Trp Val Gly Arg Leu 
        195                 200                 205             


Val Glu Arg Leu Asp Ala Lys Thr Gln Gly Lys Thr Glu Lys Glu Ala 
    210                 215                 220                 


Gly Thr Lys Leu Pro Ser Val Pro Leu Glu Glu Ile Val Ile Arg Asp 
225                 230                 235                 240 


Val Leu Thr Pro Leu Gly Leu Pro Ala Gly Ala Leu Thr Phe Ser Pro 
                245                 250                 255     


Glu Arg Tyr Pro Asp Val Phe Ala Arg Met Trp Pro Ser Leu Pro Val 
            260                 265                 270         


Arg Val Gly Asn Asn Gly Ala Leu Asp Gly Gly Pro Val Val His Gly 
        275                 280                 285             


Pro Ser Val Tyr Lys Lys Ala Pro Ala Ala Leu Gly Gly Gln Gly Met 
    290                 295                 300                 


Tyr Gly Asp Met Pro Ser Phe Phe Lys Val Ala Leu Ser Ile Phe Arg 
305                 310                 315                 320 


Asp Asp Gly Lys Leu Leu Lys Pro Glu Ser Thr Lys Leu Phe Phe Glu 
                325                 330                 335     


Pro Gln Leu Ala Ser Lys Ala Ala His Ala Gly Ile Met His Gly Thr 
            340                 345                 350         


Glu Asn Ser Gly Trp Ile Thr Gly Asp Val Pro Asp Thr Lys Glu Tyr 
        355                 360                 365             


Asp Trp Ser Val Ala Gly Leu Leu Val Thr Gly Asp Ser His Pro Phe 
    370                 375                 380                 


Arg Lys Arg Gly Ala Val Leu Trp Ala Gly Ala Phe Asn Leu Thr Trp 
385                 390                 395                 400 


Ile Ile Asp Lys Glu Ala Asp Val Cys Ala Val Phe Gly Ser Asn Tyr 
                405                 410                 415     


Gln Pro Pro Gly Asp Gln Gln Gly Lys Ala Leu Met Arg Gln Trp Glu 
            420                 425                 430         


Glu Phe Val Tyr Pro Gln Ala Lys Thr Ala Lys Leu 
        435                 440                 


<210>  3
<211>  317
<212>  PRT
<213>  Candida antartica

<400>  SEq ID No 3:  Candida antartica lipase

Leu Pro Ser Gly Ser Asp Pro Ala Phe Ser Gln Pro Lys Ser Val Leu 
1               5                   10                  15      


Asp Ala Gly Leu Thr Cys Gln Gly Ala Ser Pro Ser Ser Val Ser Lys 
            20                  25                  30          


Pro Ile Leu Leu Val Pro Gly Thr Gly Thr Thr Gly Pro Gln Ser Phe 
        35                  40                  45              


Asp Ser Asn Trp Ile Pro Leu Ser Thr Gln Leu Gly Tyr Thr Pro Cys 
    50                  55                  60                  


Trp Ile Ser Pro Pro Pro Phe Met Leu Asn Asp Thr Gln Val Asn Thr 
65                  70                  75                  80  


Glu Tyr Met Val Asn Ala Ile Thr Ala Leu Tyr Ala Gly Ser Gly Asn 
                85                  90                  95      


Asn Lys Leu Pro Val Leu Thr Trp Ser Gln Gly Gly Leu Val Ala Gln 
            100                 105                 110         


Trp Gly Leu Thr Phe Phe Pro Ser Ile Arg Ser Lys Val Asp Arg Leu 
        115                 120                 125             


Met Ala Phe Ala Pro Asp Tyr Lys Gly Thr Val Leu Ala Gly Pro Leu 
    130                 135                 140                 


Asp Ala Leu Ala Val Ser Ala Pro Ser Val Trp Gln Gln Thr Thr Gly 
145                 150                 155                 160 


Ser Ala Leu Thr Thr Ala Leu Arg Asn Ala Gly Gly Leu Thr Gln Ile 
                165                 170                 175     


Val Pro Thr Thr Asn Leu Tyr Ser Ala Thr Asp Glu Ile Val Gln Pro 
            180                 185                 190         


Gln Val Ser Asn Ser Pro Leu Asp Ser Ser Tyr Leu Phe Asn Gly Lys 
        195                 200                 205             


Asn Val Gln Ala Gln Ala Val Cys Gly Pro Leu Phe Val Ile Asp His 
    210                 215                 220                 


Ala Gly Ser Leu Thr Ser Gln Phe Ser Tyr Val Val Gly Arg Ser Ala 
225                 230                 235                 240 


Leu Arg Ser Thr Thr Gly Gln Ala Arg Ser Ala Asp Tyr Gly Ile Thr 
                245                 250                 255     


Asp Cys Asn Pro Leu Pro Ala Asn Asp Leu Thr Pro Glu Gln Lys Val 
            260                 265                 270         


Ala Ala Ala Ala Leu Leu Ala Pro Ala Ala Ala Ala Ile Val Ala Gly 
        275                 280                 285             


Pro Lys Gln Asn Cys Glu Pro Asp Leu Met Pro Tyr Ala Arg Pro Phe 
    290                 295                 300                 


Ala Val Gly Lys Arg Thr Cys Ser Gly Ile Val Thr Pro 
305                 310                 315         


