                         SEQUENCE LISTING

<110>  REGENTS OF THE UNIVERSITY OF MINNESOTA
       OHLFEST, John R.
       PANYAM, Jayanth
       SWAMINATHAN, Suresh Kumar
       VALLERA, Daniel A
 
<120>  MONOCLONAL ANTIBODY, SINGLE-CHAIN VARIABLE FRAGMENT THEREOF, AND 
       USES THEREOF

<130>  110.03290201

<150>  US 61/390,011
<151>  2010-10-05

<150>  US 61/348,348
<151>  2010-05-26

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  865
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Leu Val Leu Gly Ser Leu Leu Leu Leu Gly Leu Cys Gly Asn 
1               5                   10                  15      


Ser Phe Ser Gly Gly Gln Pro Ser Ser Thr Asp Ala Pro Lys Ala Trp 
            20                  25                  30          


Asn Tyr Glu Leu Pro Ala Thr Asn Tyr Glu Thr Gln Asp Ser His Lys 
        35                  40                  45              


Ala Gly Pro Ile Gly Ile Leu Phe Glu Leu Val His Ile Phe Leu Tyr 
    50                  55                  60                  


Val Val Gln Pro Arg Asp Phe Pro Glu Asp Thr Leu Arg Lys Phe Leu 
65                  70                  75                  80  


Gln Lys Ala Tyr Glu Ser Lys Ile Asp Tyr Asp Lys Pro Glu Thr Val 
                85                  90                  95      


Ile Leu Gly Leu Lys Ile Val Tyr Tyr Glu Ala Gly Ile Ile Leu Cys 
            100                 105                 110         


Cys Val Leu Gly Leu Leu Phe Ile Ile Leu Met Pro Leu Val Gly Tyr 
        115                 120                 125             


Phe Phe Cys Met Cys Arg Cys Cys Asn Lys Cys Gly Gly Glu Met His 
    130                 135                 140                 


Gln Arg Gln Lys Glu Asn Gly Pro Phe Leu Arg Lys Cys Phe Ala Ile 
145                 150                 155                 160 


Ser Leu Leu Val Ile Cys Ile Ile Ile Ser Ile Gly Ile Phe Tyr Gly 
                165                 170                 175     


Phe Val Ala Asn His Gln Val Arg Thr Arg Ile Lys Arg Ser Arg Lys 
            180                 185                 190         


Leu Ala Asp Ser Asn Phe Lys Asp Leu Arg Thr Leu Leu Asn Glu Thr 
        195                 200                 205             


Pro Glu Gln Ile Lys Tyr Ile Leu Ala Gln Tyr Asn Thr Thr Lys Asp 
    210                 215                 220                 


Lys Ala Phe Thr Asp Leu Asn Ser Ile Asn Ser Val Leu Gly Gly Gly 
225                 230                 235                 240 


Ile Leu Asp Arg Leu Arg Pro Asn Ile Ile Pro Val Leu Asp Glu Ile 
                245                 250                 255     


Lys Ser Met Ala Thr Ala Ile Lys Glu Thr Lys Glu Ala Leu Glu Asn 
            260                 265                 270         


Met Asn Ser Thr Leu Lys Ser Leu His Gln Gln Ser Thr Gln Leu Ser 
        275                 280                 285             


Ser Ser Leu Thr Ser Val Lys Thr Ser Leu Arg Ser Ser Leu Asn Asp 
    290                 295                 300                 


Pro Leu Cys Leu Val His Pro Ser Ser Glu Thr Cys Asn Ser Ile Arg 
305                 310                 315                 320 


Leu Ser Leu Ser Gln Leu Asn Ser Asn Pro Glu Leu Arg Gln Leu Pro 
                325                 330                 335     


Pro Val Asp Ala Glu Leu Asp Asn Val Asn Asn Val Leu Arg Thr Asp 
            340                 345                 350         


Leu Asp Gly Leu Val Gln Gln Gly Tyr Gln Ser Leu Asn Asp Ile Pro 
        355                 360                 365             


Asp Arg Val Gln Arg Gln Thr Thr Thr Val Val Ala Gly Ile Lys Arg 
    370                 375                 380                 


Val Leu Asn Ser Ile Gly Ser Asp Ile Asp Asn Val Thr Gln Arg Leu 
385                 390                 395                 400 


Pro Ile Gln Asp Ile Leu Ser Ala Phe Ser Val Tyr Val Asn Asn Thr 
                405                 410                 415     


Glu Ser Tyr Ile His Arg Asn Leu Pro Thr Leu Glu Glu Tyr Asp Ser 
            420                 425                 430         


Tyr Trp Trp Leu Gly Gly Leu Val Ile Cys Ser Leu Leu Thr Leu Ile 
        435                 440                 445             


Val Ile Phe Tyr Tyr Leu Gly Leu Leu Cys Gly Val Cys Gly Tyr Asp 
    450                 455                 460                 


Arg His Ala Thr Pro Thr Thr Arg Gly Cys Val Ser Asn Thr Gly Gly 
465                 470                 475                 480 


Val Phe Leu Met Val Gly Val Gly Leu Ser Phe Leu Phe Cys Trp Ile 
                485                 490                 495     


Leu Met Ile Ile Val Val Leu Thr Phe Val Phe Gly Ala Asn Val Glu 
            500                 505                 510         


Lys Leu Ile Cys Glu Pro Tyr Thr Ser Lys Glu Leu Phe Arg Val Leu 
        515                 520                 525             


Asp Thr Pro Tyr Leu Leu Asn Glu Asp Trp Glu Tyr Tyr Leu Ser Gly 
    530                 535                 540                 


Lys Leu Phe Asn Lys Ser Lys Met Lys Leu Thr Phe Glu Gln Val Tyr 
545                 550                 555                 560 


Ser Asp Cys Lys Lys Asn Arg Gly Thr Tyr Gly Thr Leu His Leu Gln 
                565                 570                 575     


Asn Ser Phe Asn Ile Ser Glu His Leu Asn Ile Asn Glu His Thr Gly 
            580                 585                 590         


Ser Ile Ser Ser Glu Leu Glu Ser Leu Lys Val Asn Leu Asn Ile Phe 
        595                 600                 605             


Leu Leu Gly Ala Ala Gly Arg Lys Asn Leu Gln Asp Phe Ala Ala Cys 
    610                 615                 620                 


Gly Ile Asp Arg Met Asn Tyr Asp Ser Tyr Leu Ala Gln Thr Gly Lys 
625                 630                 635                 640 


Ser Pro Ala Gly Val Asn Leu Leu Ser Phe Ala Tyr Asp Leu Glu Ala 
                645                 650                 655     


Lys Ala Asn Ser Leu Pro Pro Gly Asn Leu Arg Asn Ser Leu Lys Arg 
            660                 665                 670         


Asp Ala Gln Thr Ile Lys Thr Ile His Gln Gln Arg Val Leu Pro Ile 
        675                 680                 685             


Glu Gln Ser Leu Ser Thr Leu Tyr Gln Ser Val Lys Ile Leu Gln Arg 
    690                 695                 700                 


Thr Gly Asn Gly Leu Leu Glu Arg Val Thr Arg Ile Leu Ala Ser Leu 
705                 710                 715                 720 


Asp Phe Ala Gln Asn Phe Ile Thr Asn Asn Thr Ser Ser Val Ile Ile 
                725                 730                 735     


Glu Glu Thr Lys Lys Tyr Gly Arg Thr Ile Ile Gly Tyr Phe Glu His 
            740                 745                 750         


Tyr Leu Gln Trp Ile Glu Phe Ser Ile Ser Glu Lys Val Ala Ser Cys 
        755                 760                 765             


Lys Pro Val Ala Thr Ala Leu Asp Thr Ala Val Asp Val Phe Leu Cys 
    770                 775                 780                 


Ser Tyr Ile Ile Asp Pro Leu Asn Leu Phe Trp Phe Gly Ile Gly Lys 
785                 790                 795                 800 


Ala Thr Val Phe Leu Leu Pro Ala Leu Ile Phe Ala Val Lys Leu Ala 
                805                 810                 815     


Lys Tyr Tyr Arg Arg Met Asp Ser Glu Asp Val Tyr Asp Asp Val Glu 
            820                 825                 830         


Thr Ile Pro Met Lys Asn Met Glu Asn Gly Asn Asn Gly Tyr His Lys 
        835                 840                 845             


Asp His Val Tyr Gly Ile His Asn Pro Val Met Thr Ser Pro Ser Gln 
    850                 855                 860                 


His 
865 


<210>  2
<211>  355
<212>  PRT
<213>  Homo sapiens

<400>  2

Asn His Gln Val Arg Thr Arg Ile Lys Arg Ser Arg Lys Leu Ala Asp 
1               5                   10                  15      


Ser Asn Phe Lys Asp Leu Arg Thr Leu Leu Asn Glu Thr Pro Glu Gln 
            20                  25                  30          


Ile Lys Tyr Ile Leu Ala Gln Tyr Asn Thr Thr Lys Asp Lys Ala Phe 
        35                  40                  45              


Thr Asp Leu Asn Ser Ile Asn Ser Val Leu Gly Gly Gly Ile Leu Asp 
    50                  55                  60                  


Arg Leu Arg Pro Asn Ile Ile Pro Val Leu Asp Glu Ile Lys Ser Met 
65                  70                  75                  80  


Ala Thr Ala Ile Lys Glu Thr Lys Glu Ala Leu Glu Asn Met Asn Ser 
                85                  90                  95      


Thr Leu Lys Ser Leu His Gln Gln Ser Thr Gln Leu Ser Ser Ser Leu 
            100                 105                 110         


Thr Ser Val Lys Thr Ser Leu Arg Ser Ser Leu Asn Asp Pro Leu Cys 
        115                 120                 125             


Leu Val His Pro Ser Ser Glu Thr Cys Asn Ser Ile Arg Leu Ser Leu 
    130                 135                 140                 


Ser Gln Leu Asn Ser Asn Pro Glu Leu Arg Gln Leu Pro Pro Val Asp 
145                 150                 155                 160 


Ala Glu Leu Asp Asn Val Asn Asn Val Leu Arg Thr Asp Leu Asp Gly 
                165                 170                 175     


Leu Val Gln Gln Gly Tyr Gln Ser Leu Asn Asp Ile Pro Asp Arg Val 
            180                 185                 190         


Gln Arg Gln Thr Thr Thr Val Val Ala Ala Ala Gly Arg Lys Asn Leu 
        195                 200                 205             


Gln Asp Phe Ala Ala Cys Gly Ile Asp Arg Met Asn Tyr Asp Ser Tyr 
    210                 215                 220                 


Leu Ala Gln Thr Gly Lys Ser Pro Ala Gly Val Asn Leu Leu Ser Phe 
225                 230                 235                 240 


Ala Tyr Asp Leu Glu Ala Lys Ala Asn Ser Leu Pro Pro Gly Asn Leu 
                245                 250                 255     


Arg Asn Ser Leu Lys Arg Asp Ala Gln Thr Ile Lys Thr Ile His Gln 
            260                 265                 270         


Gln Arg Val Leu Pro Ile Glu Gln Ser Leu Ser Thr Leu Tyr Gln Ser 
        275                 280                 285             


Val Lys Ile Leu Gln Arg Thr Gly Asn Gly Leu Leu Glu Arg Val Thr 
    290                 295                 300                 


Arg Ile Leu Ala Ser Leu Asp Phe Ala Gln Asn Phe Ile Thr Asn Asn 
305                 310                 315                 320 


Thr Ser Ser Val Ile Ile Glu Glu Thr Lys Lys Tyr Gly Arg Thr Ile 
                325                 330                 335     


Ile Gly Tyr Phe Glu His Tyr Leu Gln Trp Ile Glu Phe Ser Ile Ser 
            340                 345                 350         


Glu Lys Val 
        355 


<210>  3
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  3
gggcccaggc ggccgagctc gayatccagc tgactcagcc                             40


<210>  4
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  4
gggcccaggc ggccgagctc gayattgttc tcwcccagtc                             40


<210>  5
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  5
gggcccaggc ggccgagctc gayattgtgm tmactcagtc                             40


<210>  6
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  6
gggcccaggc ggccgagctc gayattgtgy tracacagtc                             40


<210>  7
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  7
gggcccaggc ggccgagctc gayattgtra tgacmcagtc                             40


<210>  8
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  8
gggcccaggc ggccgagctc gayattmaga tramccagtc                             40


<210>  9
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  9
gggcccaggc ggccgagctc gayattcaga tgaydcagtc                             40


<210>  10
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  10
gggcccaggc ggccgagctc gayatycaga tgacacagac                             40


<210>  11
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  11
gggcccaggc ggccgagctc gayattgttc tcawccagtc                             40


<210>  12
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  12
gggcccaggc ggccgagctc gayattgwgc tsacccaatc                             40


<210>  13
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  13
gggcccaggc ggccgagctc gayattstra tgacccartc                             40


<210>  14
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  14
gggcccaggc ggccgagctc gayrttktga tgacccarac                             40


<210>  15
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  15
gggcccaggc ggccgagctc gayattgtga tgacbcagkc                             40


<210>  16
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  16
gggcccaggc ggccgagctc gayattgtga taacycagga                             40


<210>  17
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  17
gggcccaggc ggccgagctc gayattgtga tgacccagwt                             40


<210>  18
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  18
gggcccaggc ggccgagctc gayattgtga tgacacaacc                             40


<210>  19
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  19
gggcccaggc ggccgagctc gayattttgc tgactcagtc                             40


<210>  20
<211>  75
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  20
ggaagatcta gaggaaccac ccccaccacc gcccgagcca ccgccaccag aggatttkat       60

ttccagyttg gtccc                                                        75


<210>  21
<211>  75
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  21
ggaagatcta gaggaaccac ccccaccacc gcccgagcca ccgccaccag aggattttat       60

ttccaacttt gtccc                                                        75


<210>  22
<211>  75
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  22
ggaagatcta gaggaaccac ccccaccacc gcccgagcca ccgccaccag aggatttcag       60

ctccagcttg gtccc                                                        75


<210>  23
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  23
ggtggttcct ctagatcttc cctcgaggtr magcttcagg agtc                        44


<210>  24
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  24
ggtggttcct ctagatcttc cctcgaggtb cagctbcagc agtc                        44


<210>  25
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  25
ggtggttcct ctagatcttc cctcgaggtg cagctgaags astc                        44


<210>  26
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  26
ggtggttcct ctagatcttc cctcgaggtc carctgcaac artc                        44


<210>  27
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  27
ggtggttcct ctagatcttc cctcgaggty cagctbcagc artc                        44


<210>  28
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  28
ggtggttcct ctagatcttc cctcgaggty carctgcagc agtc                        44


<210>  29
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  29
ggtggttcct ctagatcttc cctcgaggtc cacgtgaagc agtc                        44


<210>  30
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  30
ggtggttcct ctagatcttc cctcgaggtg aasstggtgg aatc                        44


<210>  31
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  31
ggtggttcct ctagatcttc cctcgaggtg awgytggtgg agtc                        44


<210>  32
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  32
ggtggttcct ctagatcttc cctcgaggtg cagskggtgg agtc                        44


<210>  33
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  33
ggtggttcct ctagatcttc cctcgaggtg camctggtgg agtc                        44


<210>  34
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  34
ggtggttcct ctagatcttc cctcgaggtg aagctgatgg artc                        44


<210>  35
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  35
ggtggttcct ctagatcttc cctcgaggtg carcttgttg agtc                        44


<210>  36
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  36
ggtggttcct ctagatcttc cctcgaggtr aagcttctcg agtc                        44


<210>  37
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  37
ggtggttcct ctagatcttc cctcgaggtg aarsttgagg agtc                        44


<210>  38
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  38
ggtggttcct ctagatcttc cctcgaggtt actctraaag wgtstg                      46


<210>  39
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  39
ggtggttcct ctagatcttc cctcgaggtc caactvcagc arcc                        44


<210>  40
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  40
ggtggttcct ctagatcttc cctcgaggtg aacttggaag tgtc                        44


<210>  41
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  41
ggtggttcct ctagatcttc cctcgaggtg aaggtcatcg agtc                        44


<210>  42
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  42
cctggccggc ctggccacta gtgacagatg gggstgtygt tttggc                      46


<210>  43
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  43
gaggaggagg aggaggaggc ggggcccagg cggccgagct c                           41


<210>  44
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  44
gaggaggagg aggaggagcc tggccggcct ggccactagt g                           41


<210>  45
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  45
aagacagcta tcgcgattgc ag                                                22


<210>  46
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  46
gcccccttat tagcgtttgc catc                                              24


<210>  47
<211>  260
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(260)
<223>  variable region of the heavy chain

<400>  47

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Gly Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Pro Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln 
            260 


<210>  48
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  48

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  49
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  49

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Pro Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Pro Glu Val Met Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  50
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  50

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Gln Pro 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Leu Val Ser Asn Leu Glu Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile 
65                  70                  75                  80  


His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  51
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  51

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Pro Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val His Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  52
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  52

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Ser Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Pro 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Cys Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  53
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  53

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Ser Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  54
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  54

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Pro Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Asp Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  55
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  MISC_FEATURE
<222>  (1)..(112)
<223>  variable region of the light chain

<220>
<221>  MISC_FEATURE
<222>  (113)..(130)
<223>  linker

<220>
<221>  MISC_FEATURE
<222>  (131)..(263)
<223>  variable region of the heavy chain

<400>  55

Ala Gln Ala Ala Glu Leu Asp Ile Val Leu Thr Gln Ser Pro Ala Ile 
1               5                   10                  15      


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser 
            20                  25                  30          


Ser Ser Val Ser Tyr Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser 
        35                  40                  45              


Pro Lys Pro Trp Ile Tyr Arg Pro Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr 
                85                  90                  95      


His Ser Tyr Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
        115                 120                 125             


Ser Ser Leu Glu Val Gln Leu Val Glu Ser Gly Pro Glu Leu Lys Lys 
    130                 135                 140                 


Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Ser Met His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu 
                165                 170                 175     


Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala 
            180                 185                 190         


Asp Asp Phe Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser 
        195                 200                 205             


Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr 
    210                 215                 220                 


Tyr Phe Cys Ala Thr Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
225                 230                 235                 240 


Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Ala Pro Ser Val 
                245                 250                 255     


Thr Ser Gly Gln Ala Gly Gln 
            260             


<210>  56
<211>  846
<212>  DNA
<213>  artificial

<220>
<223>  single-chain variable-fragment (scFv) of a monoclonal antibody 
       clone


<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  SfiI restriction site

<220>
<221>  misc_feature
<222>  (337)..(390)
<223>  linker

<220>
<221>  misc_feature
<222>  (775)..(787)
<223>  SfiI restriction site

<220>
<221>  misc_feature
<222>  (790)..(807)
<223>  6HIS tag

<220>
<221>  misc_feature
<222>  (814)..(843)
<223>  HA tag

<220>
<221>  terminator
<222>  (844)..(846)

<400>  56
ggcccaggcg gccgagctcg acattgttct ctcccagtct ccagcaatca tgtctgcatc       60

tccaggggag aaggtcacca tatcctgcag tgccagctca agtgtaagtt atatgtactg      120

gtaccagcag aaccaggatc ctcccccaaa ccctggattt atcgcacatc caacctggct      180

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc      240

agcagcatgg aggctgaaga tgctgccact tattactgcc agcagtatca tagttaccca      300

cccacgttcg gtgctgggac caagctggag ctgaaatcct ctggtggcgg tggctcgggc      360

ggtggtgggg gtggttcctc tagatcttcc ctcgaggtga agctggtgga gtctggacct      420

gagctgaaga agcctggaga gacagtcaag atctcctgca aggcttctgg ttataccttc      480

acagactatt caatgcactg ggtgaatcag gctccaggaa agggtttaaa gtggatgggc      540

tggataaaca ctgagactgg tgagccatca tatgcagatg acttcaaggg acggtttgcc      600

ttctctttgg aaacctctgc cagcactgcc tatttgcaga tcaacaacct caaaaatgag      660

gacacggcta catatttctg tgctaccgat tacggggact actttgacta ctggggccaa      720

ggcaccactc tcacagtctc ctcagccaaa acgacacccc catctgtcac tagtggccag      780

gccggccagc accatcacca tcaccatggc gcatacccgt acgacgttcc ggactacgct      840

tcttag                                                                 846


<210>  57
<211>  729
<212>  DNA
<213>  artificial

<220>
<223>  scFv polypeptide encoding sequence

<400>  57
atggacattg ttctctccca gtctccagca atcatgtctg catctccagg ggagaaggtc       60

accatatcct gcagtgccag ctcaagtgta agttatatgt actggtacca gcagaagcca      120

ggatcctccc ccaaaccctg gatttatcgc acatccaacc tggcttctgg agtccctgct      180

cgcttcagtg gcagtgggtc tgggacctct tactctctca caatcagcag catggaggct      240

gaagatgctg ccacttatta ctgccagcag tatcatagtt acccacccac gttcggtgct      300

gggaccaagc tggagctgaa atcctctggt ggcggtggct cgggcggtgg tgggggtggt      360

tcctctagat cttccctcga ggtgaagctg gtggagtctg gacctgagct gaagaagcct      420

ggagagacag tcaagatctc ctgcaaggct tctggttata ccttcacaga ctattcaatg      480

cactgggtga atcaggctcc aggaaagggt ttaaagtgga tgggctggat aaacactgag      540

actggtgagc catcatatgc agatgacttc aagggacggt ttgccttctc tttggaaacc      600

tctgccagca ctgcctattt gcagatcaac aacctcaaaa atgaggacac ggctacatat      660

ttctgtgcta ccgattacgg ggactacttt gactactggg gccaaggcac cactctcaca      720

gtctcctca                                                              729


<210>  58
<211>  243
<212>  PRT
<213>  artificial

<220>
<223>  scFv polypeptide

<400>  58

Met Asp Ile Val Leu Ser Gln Ser Pro Ala Ile Met Ser Ala Ser Pro 
1               5                   10                  15      


Gly Glu Lys Val Thr Ile Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr 
            20                  25                  30          


Met Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys Pro Trp Ile 
        35                  40                  45              


Tyr Arg Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr His Ser Tyr Pro Pro 
                85                  90                  95      


Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Ser Ser Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Leu Glu Val 
        115                 120                 125             


Lys Leu Val Glu Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val 
    130                 135                 140                 


Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Ser Met 
145                 150                 155                 160 


His Trp Val Asn Gln Ala Pro Gly Lys Gly Leu Lys Trp Met Gly Trp 
                165                 170                 175     


Ile Asn Thr Glu Thr Gly Glu Pro Ser Tyr Ala Asp Asp Phe Lys Gly 
            180                 185                 190         


Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr Leu Gln 
        195                 200                 205             


Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys Ala Thr 
    210                 215                 220                 


Asp Tyr Gly Asp Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
225                 230                 235                 240 


Val Ser Ser 
            


<210>  59
<211>  159
<212>  DNA
<213>  Homo sapiens

<400>  59
aacagcgaca gcgaatgtcc gctgagccac gacggttact gtctgcacga cggtgtttgt       60

atgtacatcg aagctctaga caaatacgct tgtaactgtg ttgttggtta catcggtgaa      120

cgctgtcagt accgcgacct gaaatggtgg gaactgcgc                             159


<210>  60
<211>  53
<212>  PRT
<213>  Homo sapiens

<400>  60

Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His 
1               5                   10                  15      


Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn 
            20                  25                  30          


Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys 
        35                  40                  45              


Trp Trp Glu Leu Arg 
    50              


<210>  61
<211>  1170
<212>  DNA
<213>  Homo sapiens

<400>  61
atgggcgctg atgatgttgt tgattcttct aaatcttttg tgatggaaaa cttttcttcg       60

taccacggga ctaaacctgg ttatgtagat tccattcaaa aaggtataca aaagccaaaa      120

tctggtacac aaggaaatta tgacgatgat tggaaagggt tttatagtac cgacaataaa      180

tacgacgctg cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc      240

gtggtcaaag tgacgtatcc aggactgacg aaggttctcg cactaaaagt ggataatgcc      300

gaaactatta agaaagagtt aggtttaagt ctcactgaac cgttgatgga gcaagtcgga      360

acggaagagt ttatcaaaag gttcggtgat ggtgcttcgc gtgtagtgct cagccttccc      420

ttcgctgagg ggagttctag cgttgaatat attaataact gggaacaggc gaaagcgtta      480

agcgtagaac ttgagattaa ttttgaaacc cgtggaaaac gtggccaaga tgcgatgtat      540

gagtatatgg ctcaagcctg tgcaggaaat cgtgtcaggc gatcagtagg tagctcattg      600

tcatgcataa atcttgattg ggatgtcata agggataaaa ctaagacaaa gatagagtct      660

ttgaaagagc atggccctat caaaaataaa atgagcgaaa gtcccaataa aacagtatct      720

gaggaaaaag ctaaacaata cctagaagaa tttcatcaaa cggcattaga gcatcctgaa      780

ttgtcagaac ttaaaaccgt tactgggacc aatcctgtat tcgctggggc taactatgcg      840

gcgtgggcag taaacgttgc gcaagttatc gatagcgaaa cagctgataa tttggaaaag      900

acaactgctg ctctttcgat acttcctggt atcggtagcg taatgggcat tgcagacggt      960

gccgttcacc acaatacaga agagatagtg gcacaatcaa tagctttatc gtctttaatg     1020

gttgctcaag ctattccatt ggtaggagag ctagttgata ttggtttcgc tgcatataat     1080

tttgtagaga gtattatcaa tttatttcaa gtagttcata attcgtataa tcgtcccgcg     1140

tattctccgg ggcataaaac gcaaccattt                                      1170


<210>  62
<211>  390
<212>  PRT
<213>  Homo sapiens

<400>  62

Met Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu 
1               5                   10                  15      


Asn Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile 
            20                  25                  30          


Gln Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp 
        35                  40                  45              


Asp Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala 
    50                  55                  60                  


Gly Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly 
65                  70                  75                  80  


Val Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys 
                85                  90                  95      


Val Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr 
            100                 105                 110         


Glu Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe 
        115                 120                 125             


Gly Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly 
    130                 135                 140                 


Ser Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu 
145                 150                 155                 160 


Ser Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln 
                165                 170                 175     


Asp Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val 
            180                 185                 190         


Arg Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp 
        195                 200                 205             


Val Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His 
    210                 215                 220                 


Gly Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser 
225                 230                 235                 240 


Glu Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu 
                245                 250                 255     


Glu His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro 
            260                 265                 270         


Val Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln 
        275                 280                 285             


Val Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala 
    290                 295                 300                 


Leu Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly 
305                 310                 315                 320 


Ala Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu 
                325                 330                 335     


Ser Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val 
            340                 345                 350         


Asp Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu 
        355                 360                 365             


Phe Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
    370                 375                 380                 


His Lys Thr Gln Pro Phe 
385                 390 


<210>  63
<211>  336
<212>  DNA
<213>  Homo sapiens

<400>  63
ggccctgtgc ctccctctac agccctcagg gagctcattg aggagctggt caacatcacc       60

cagaaccaga aggctccgct ctgcaatggc agcatggtat ggagcatcaa cctgacagct      120

ggcatgtact gtgcagccct ggaatccctg atcaacgtgt caggctgcag tgccatcgag      180

aagacccaga ggatgctgag cggattctgc ccgcacaagg tctcagctgg gcagttttcc      240

agcttgcatg tccgagacac caaaatcgag gtggcccagt ttgtaaagga cctgctctta      300

catttaaaga aactttttcg cgagggacgg ttcaac                                336


<210>  64
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  64

Gly Pro Val Pro Pro Ser Thr Ala Leu Arg Glu Leu Ile Glu Glu Leu 
1               5                   10                  15      


Val Asn Ile Thr Gln Asn Gln Lys Ala Pro Leu Cys Asn Gly Ser Met 
            20                  25                  30          


Val Trp Ser Ile Asn Leu Thr Ala Gly Met Tyr Cys Ala Ala Leu Glu 
        35                  40                  45              


Ser Leu Ile Asn Val Ser Gly Cys Ser Ala Ile Glu Lys Thr Gln Arg 
    50                  55                  60                  


Met Leu Ser Gly Phe Cys Pro His Lys Val Ser Ala Gly Gln Phe Ser 
65                  70                  75                  80  


Ser Leu His Val Arg Asp Thr Lys Ile Glu Val Ala Gln Phe Val Lys 
                85                  90                  95      


Asp Leu Leu Leu His Leu Lys Lys Leu Phe Arg Glu Gly Arg Phe Asn 
            100                 105                 110         


<210>  65
<211>  1086
<212>  DNA
<213>  Homo sapiens

<400>  65
cccgagggcg gcagcctggc cgcgctgacc gcgcaccagg cttgccacct gccgctggag       60

actttcaccc gtcatcgcca gccgcgcggc tgggaacaac tggagcagtg cggctatccg      120

gtgcagcggc tggtcgccct ctacctggcg gcgcggctgt cgtggaacca ggtcgaccag      180

gtgatccgca acgccctggc cagccccggc agcggcggcg acctgggcga agcgatccgc      240

gagcagccgg agcaggcccg tctggccctg accctggccg ccgccgagag cgagcgcttc      300

gtccggcagg gcaccggcaa cgacgaggcc ggcgcggcca acgccgacgt ggtgagcctg      360

acctgcccgg tcgccgccgg tgaatgcgcg ggcccggcgg acagcggcga cgccctgctg      420

gagcgcaact atcccactgg cgcggagttc ctcggcgacg gcggcgacgt cagcttcagc      480

acccgcggca cgcagaactg gacggtggag cggctgctcc aggcgcaccg ccaactggag      540

gagcgcggct atgtgttcgt cggctaccac ggcaccttcc tcgaagcggc gcaaagcatc      600

gtcttcggcg gggtgcgcgc gcgcagccag gacctcgacg cgatctggcg cggtttctat      660

atcgccggcg atccggcgct ggcctacggc tacgcccagg accaggaacc cgacgcacgc      720

ggccggatcc gcaacggtgc cctgctgcgg gtctatgtgc cgcgctcgag cctgccgggc      780

ttctaccgca ccagcctgac cctggccgcg ccggaggcgg cgggcgaggt cgaacggctg      840

atcggccatc cgctgccgct gcgcctggac gccatcaccg gccccgagga ggaaggcggg      900

cgcctggaga ccattctcgg ctggccgctg gccgagcgca ccgtggtgat tccctcggcg      960

atccccaccg acccgcgcaa cgtcggcggc gacctcgacc cgtccagcat ccccgacaag     1020

gaacaggcga tcagcgccct gccggactac gccagccagc ccggcaaacc gccgaaggac     1080

gagcta                                                                1086


<210>  66
<211>  362
<212>  PRT
<213>  Homo sapiens

<400>  66

Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
1               5                   10                  15      


Leu Pro Leu Glu Thr Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu 
            20                  25                  30          


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
        35                  40                  45              


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
    50                  55                  60                  


Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
65                  70                  75                  80  


Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
                85                  90                  95      


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
            100                 105                 110         


Ala Asn Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu 
        115                 120                 125             


Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr 
    130                 135                 140                 


Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser 
145                 150                 155                 160 


Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His 
                165                 170                 175     


Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr 
            180                 185                 190         


Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg 
        195                 200                 205             


Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp 
    210                 215                 220                 


Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg 
225                 230                 235                 240 


Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Ser 
                245                 250                 255     


Ser Leu Pro Gly Phe Tyr Arg Thr Ser Leu Thr Leu Ala Ala Pro Glu 
            260                 265                 270         


Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg 
        275                 280                 285             


Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Leu Glu Thr 
    290                 295                 300                 


Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala 
305                 310                 315                 320 


Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser 
                325                 330                 335     


Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser 
            340                 345                 350         


Gln Pro Gly Lys Pro Pro Lys Asp Glu Leu 
        355                 360         


<210>  67
<211>  1107
<212>  DNA
<213>  Homo sapiens

<400>  67
cccgagggcg gcagcctggc cgcgctgacc gcgcaccagg cttgccacct gccgctggag       60

actttcaccc gtcatcgcca gccgcgcggc tgggaacaac tggagcagtg cggctatccg      120

gtgcagcggc tggtcgccct ctacctggcg gcgcggctgt cgtggaacca ggtcgaccag      180

gtgatcgcca acgccctggc cagccccggc agcggcggcg acctgggcga agcgatccgc      240

gagtcgccgg agcaggcccg tctggccctg accctggccg ccgccgagag cgagcgcttc      300

gtccggcagg gcaccggcaa cgacgaggcc ggcgcggcca acgccgacgt ggtgagcctg      360

acctgcccgg tcgccgccgg tgaatgcgcg ggcccggcgg acagcggcga cgccctgctg      420

gagcgcaact atcccactgg cgcggagttc ctcggcgacg gcggcgacgt cagcttcagc      480

acccgcggca cgcagaactg gacggtggag cggctgctcc aggcgcaccg ccaactggag      540

gagggaggct atgtgttcgt cggctaccac ggcaccttcc tcgaagcggc gcaaagcatc      600

gtcttcggcg gggtgcgcgc gcgcagccag gacctcgacg cgatctgggc cggtttctat      660

atcgccggcg atccggcgct ggcctacggc tacgcccagg accaggaacc cgacgcagcc      720

ggccggatcc gcaacggtgc cctgctgcgg gtctatgtgc cgcgctcgag cctgccgggc      780

ttctacgcca ccagcctgac cctggccgcg ccggaggcgg cgggcgaggt cgaacggctg      840

atcggccatc cgctgccgct gcgcctggac gccatcaccg gccccgagga gtcaggcggg      900

cgcctggaga ccattctcgg ctggccgctg gccgagcgca ccgtggtgat tccctcggcg      960

atccccaccg acccgcgcaa cgtcggcggc gacctcgacc cgtccagcat ccccgactcg     1020

gaacaggcga tcagcgccct gccggactac gccagccagc ccggcaaacc gccgaaggac     1080

gagctagaag cttccggagg tcccgag                                         1107


<210>  68
<211>  369
<212>  PRT
<213>  Homo sapiens

<400>  68

Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
1               5                   10                  15      


Leu Pro Leu Glu Thr Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu 
            20                  25                  30          


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
        35                  40                  45              


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Ala Asn 
    50                  55                  60                  


Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
65                  70                  75                  80  


Glu Ser Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
                85                  90                  95      


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
            100                 105                 110         


Ala Asn Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu 
        115                 120                 125             


Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr 
    130                 135                 140                 


Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser 
145                 150                 155                 160 


Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His 
                165                 170                 175     


Arg Gln Leu Glu Glu Gly Gly Tyr Val Phe Val Gly Tyr His Gly Thr 
            180                 185                 190         


Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg 
        195                 200                 205             


Ser Gln Asp Leu Asp Ala Ile Trp Ala Gly Phe Tyr Ile Ala Gly Asp 
    210                 215                 220                 


Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Ala 
225                 230                 235                 240 


Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Ser 
                245                 250                 255     


Ser Leu Pro Gly Phe Tyr Ala Thr Ser Leu Thr Leu Ala Ala Pro Glu 
            260                 265                 270         


Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg 
        275                 280                 285             


Leu Asp Ala Ile Thr Gly Pro Glu Glu Ser Gly Gly Arg Leu Glu Thr 
    290                 295                 300                 


Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala 
305                 310                 315                 320 


Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser 
                325                 330                 335     


Ile Pro Asp Ser Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser 
            340                 345                 350         


Gln Pro Gly Lys Pro Pro Lys Asp Glu Leu Glu Ala Ser Gly Gly Pro 
        355                 360                 365             


Glu 
    


<210>  69
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  ARL linker

<400>  69

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  70
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  endoplasmic reticulum retention sequence

<400>  70

Lys Asp Glu Leu 
1               


<210>  71
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  G4S linker

<400>  71

Gly Gly Gly Gly Ser 
1               5   


<210>  72
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  seven amino acid linker

<400>  72

Glu Ala Ser Gly Gly Pro Glu 
1               5           


<210>  73
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  C-terminal of PE38

<400>  73

Arg Glu Asp Leu Lys 
1               5   


<210>  74
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  linker peptide

<400>  74

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
1               5                   10                  15      


Ser Ser 
        


