                         SEQUENCE LISTING

<110>  PHARMAZELL GmbH
 
<120>  7alpha-Hydroxysterioddehydrogenase-Knock-Out-Mutanten

<130>  M/50273-PCT

<160>  38    

<170>  PatentIn version 3.3

<210>  1
<211>  4334
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid

<400>  1
gtgtaggctg gagctgcttc gaagttccta tactttctag agaataggaa cttcggaata     60

ggaacttatg agctcagcca atcgactggc gagcggcatc gcattcttcg catcccgcct    120

ctggcggatg caggaagatc aacggatctc ggcccagttg acccagggct gtcgccacaa    180

tgtcgcggga gcggatcaac cgagcaaagg catgaccgac tggaccttcc ttctgaaggc    240

tcttctcctt gagccacctg tccgccaagg caaagcgctc acagcagtgg tcattctcga    300

gataatcgac gcgtaccaac ttgccatcct gaagaatggt gcagtgtctc ggcaccccat    360

agggaacctt tgccatcaac tcggcaagat gcagcgtcgt gttggcatcg tgtcccacgc    420

cgaggagaag tacctgccca tcgagttcat ggacacgggc gaccgggctt gcaggcgagt    480

gaggtggcag gggcaatgga tcagagatga tctgctctgc ctgtggcccc gctgccgcaa    540

aggcaaatgg atgggcgctg cgctttacat ttggcaggcg ccagaatgtg tcagagacaa    600

ctccaaggtc cggtgtaacg ggcgacgtgg caggatcgaa cggctcgtcg tccagacctg    660

accacgaggg catgacgagc gtccctcccg gacccagcgc agcacgcagg gcctcgatca    720

gtccaagtgg cccatcttcg aggggccgga cgctacggaa ggagctgtgg accagcagca    780

caccgccggg ggtaacccca aggttgagaa gctgaccgat gagctcggct tttcgccatt    840

cgtattgcac gacattgcac tccaccgctg atgacatcag tcgatcatag cacgatcaac    900

ggcactgttg caaatagtcg gtggtgataa acttatcatc cccttttgct gatggagctg    960

cacatgaacc cattcaaagg ccggcatttt cagcgtgaca tcattctgtg ggccgtacgc   1020

tggtactgca aatacggcat cagttaccgt gagctgcatt ttccgctgca taaccctgct   1080

tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat   1140

tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga   1200

taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc   1260

tggcggtgct caacgggaat cctgctctgc gaggctggcg ggaacttcga agttcctata   1320

ctttctagag aataggaact tcgaactgca ggtcgacgga tccccggaat atcaagctta   1380

tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat   1440

tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa   1500

cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc   1560

accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt   1620

agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc   1680

agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc   1740

tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg   1800

cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga   1860

tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc   1920

caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg   1980

ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt   2040

aacaaaatat taacgtttac aatttcccag gtggcacttt tcggggaaat gtgcgcggaa   2100

cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac   2160

cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg   2220

tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc   2280

tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg   2340

atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga   2400

gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc   2460

aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag   2520

aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga   2580

gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg   2640

cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga   2700

atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt   2760

tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact   2820

ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt   2880

ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg   2940

ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta   3000

tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac   3060

tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta   3120

aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt   3180

tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt   3240

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt   3300

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc   3360

agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg   3420

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg   3480

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt   3540

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac   3600

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg   3660

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg   3720

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat   3780

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt   3840

tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg   3900

attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa   3960

cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc   4020

ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga   4080

aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg   4140

ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc   4200

acacaggaaa cagctatgac catgattacg ccaagctcgg aattaaccct cactaaaggg   4260

aacaaaagct ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccgggc   4320

tgcaggaatt cgat                                                     4334


<210>  2
<211>  7220
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid

<400>  2
catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac     60

ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat    120

cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca    180

gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct    240

ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga    300

tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat    360

tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct    420

caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga    480

tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg    540

tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt    600

aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc    660

ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca    720

ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt    780

cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg    840

cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac    900

tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg    960

tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt   1020

aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca   1080

gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat   1140

ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat   1200

acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga   1260

aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc   1320

atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat   1380

tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga   1440

gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc   1500

gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt   1560

tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac   1620

cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc   1680

gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa   1740

ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc   1800

gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc   1860

tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc   1920

atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca   1980

tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa   2040

tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg   2100

catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga   2160

tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact   2220

gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt   2280

aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc   2340

cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa   2400

gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca   2460

ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc   2520

acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc   2580

gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg   2640

tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg   2700

agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga   2760

tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg   2820

gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg   2880

gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga   2940

cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc   3000

attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg   3060

agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat   3120

ggcgatgata agctagctta aaaaagcaaa agggccgcag atgcgaccct tgtgtatcaa   3180

acaagacgat taaaaatctt cgttagtttc tgctacgcct tcgctatcat ctacagagaa   3240

atccggcgtt gagttcgggt tgctcagcag caactcacgt actttcttct cgatctcttt   3300

cgcggtttcc gggttatctt tcagccaggc agtcgcattc gctttaccct gaccgatctt   3360

ctcacctttg tagctgtacc acgcgcctgc tttctcgatc agcttctctt ttacgcccag   3420

gtcaaccagt tcgccgtaga agttgatacc ttcgccgtag aggatctgga attcagcctg   3480

tttaaacggc gcagcgattt tgttcttcac cactttcacg cgggtttcgc tacccaccac   3540

gttttcgccc tctttcaccg cgccgatacg acggatgtcg agacgaacag aggcgtagaa   3600

tttcagcgcg ttaccaccgg tagtggtttc cgggttaccg aacatcacac caattttcat   3660

acggatctgg ttgatgaaga tcagcagcgt gttggactgc ttcaggttac ccgccagctt   3720

acgcatcgcc tggctcatca tacgtgccgc aaggcccatg tgagagtcgc cgatttcgcc   3780

ttcgatttcc gctttcggcg tcagtgccgc cacggagtca acgacgataa cgtctactgc   3840

gccagaacgc gccagggcgt cacagatttc cagtgcctgc tcgccggtgt ccggctggga   3900

gcacagcagg ttgtcgatat cgacgcccag tttacgtgcg tagattgggt ccagcgcgtg   3960

ttcagcatcg ataaacgcac aggttttacc ttcacgctgc gctgcggcga tcacctgcag   4020

cgtcagcgtg gttttaccgg aagattccgg tccgtagatt tcgacgatac ggcccatcgg   4080

cagaccacct gccccaagcg cgatatccag tgaaagcgaa ccggtagaga tggtttccac   4140

atccatggaa cggtcttcac ccaggcgcat gatggagcct ttaccaaatt gtttctcaat   4200

ctggcccagt gctgccgcca acgctttctg tttgttttcg tcgatagcca tttttactcc   4260

tgtcatgcct agcccatggg tatggacagt tttccctttg atatgtaacg gtgaacagtt   4320

gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc   4380

tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag   4440

ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc   4500

aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt   4560

acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg   4620

gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa   4680

cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa   4740

atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt   4800

ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt   4860

tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc   4920

aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg   4980

caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt   5040

ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat   5100

cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat   5160

ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat   5220

cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc   5280

atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa   5340

ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact   5400

agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact   5460

tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta   5520

tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga   5580

tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc   5640

gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac   5700

cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg   5760

accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct   5820

ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa   5880

ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg   5940

ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc   6000

tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc   6060

agcggtatca tcaacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt   6120

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt   6180

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag   6240

tgaggcacct atctcagcga ttaagggcac caataactgc cttaaaaaaa ttacgccccg   6300

ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca   6360

tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta   6420

taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa   6480

tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac   6540

cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt   6600

agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc   6660

tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc   6720

attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc   6780

ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga   6840

acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga   6900

tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc   6960

ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta   7020

tggtgaaagt tggaacctct tacgtgccga aaagggaata agggcgacac ggaaatgttg   7080

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat   7140

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt   7200

tccccgaaaa gtgccacctg                                               7220


<210>  3
<211>  59
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  3
gtgtttaatt ctgacaacct gagactcgac ggaaaaatga ttccggggat ccgtcgacc      59


<210>  4
<211>  58
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  4
ttaattgagc tcctgtaccc caccaccgga gacggtttat gtaggctgga gctgcttc       58


<210>  5
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  5
ggtattccat atgatctttg acggaaaggt cgc                                  33


<210>  6
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  6
cgggatccct aggggcgctg accc                                            24


<210>  7
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  7
gtttaacttt aagaaggaga tataccatga tctttgacgg aaaggtcgca atcatt         56


<210>  8
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  8
aatgattgcg acctttccgt caaagatcat ggtatatctc cttcttaaag ttaaac         56


<210>  9
<211>  768
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(768)

<400>  9
gtg ttt aat tct gac aac ctg aga ctc gac gga aaa tgc gcc atc atc       48
Val Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile         
1               5                   10                  15              

aca ggt gcg ggt gca ggt att ggt aaa gaa atc gcc att aca ttc gcg       96
Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala         
            20                  25                  30                  

aca gct ggc gca tct gtg gtg gtc agt gat att aac gcc gac gca gct      144
Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala         
        35                  40                  45                      

aac cat gtt gta gac gaa att caa caa ctg ggt ggt cag gca ttt gcc      192
Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala         
    50                  55                  60                          

tgc cgt tgt gat att act tcc gaa cag gaa ctc tct gca ctg gca gac      240
Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp         
65                  70                  75                  80          

ttt gct atc agt aag ctg ggt aaa gtt gat att ctg gtt aac aac gcc      288
Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala         
                85                  90                  95              

ggt ggc ggt gga cct aaa ccg ttt gat atg cca atg gcg gat ttt cgc      336
Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg         
            100                 105                 110                 

cgt gct tat gaa ctg aat gtg ttt tct ttt ttc cat ctg tca caa ctt      384
Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu         
        115                 120                 125                     

gtt gcg cca gaa atg gaa aaa aat ggc ggt ggc gtt att ctg acc atc      432
Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile         
    130                 135                 140                         

act tct atg gcg gca gaa aat aaa aat ata aac atg act tcc tat gca      480
Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala         
145                 150                 155                 160         

tca tct aaa gct gcg gcc agt cat ctg gtc aga aat atg gcg ttt gac      528
Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp         
                165                 170                 175             

cta ggt gaa aaa aat att cgg gta aat ggc att gcg ccg ggg gca ata      576
Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile         
            180                 185                 190                 

tta acc gat gcc ctg aaa tcc gtt att aca cca gaa att gaa caa aaa      624
Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys         
        195                 200                 205                     

atg tta cag cac acg ccg atc aga cgt ctg ggc caa ccg caa gat att      672
Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile         
    210                 215                 220                         

gct aac gca gcg ctg ttc ctt tgc tcg cct gct gcg agc tgg gta agc      720
Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser         
225                 230                 235                 240         

gga caa att ctc acc gtc tcc ggt ggt ggg gta cag gag ctc aat taa      768
Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn             
                245                 250                 255             


<210>  10
<211>  255
<212>  PRT
<213>  Escherichia coli

<400>  10

Val Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile 
1               5                   10                  15      


Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala 
            20                  25                  30          


Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala 
        35                  40                  45              


Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 
    50                  55                  60                  


Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 
65                  70                  75                  80  


Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 
                85                  90                  95      


Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg 
            100                 105                 110         


Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu 
        115                 120                 125             


Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile 
    130                 135                 140                 


Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala 
145                 150                 155                 160 


Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 
                165                 170                 175     


Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 
            180                 185                 190         


Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 
        195                 200                 205             


Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 
    210                 215                 220                 


Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser 
225                 230                 235                 240 


Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn 
                245                 250                 255 


<210>  11
<211>  813
<212>  DNA
<213>  Clostridium sp.


<220>
<221>  CDS
<222>  (1)..(813)

<400>  11
atg gat ttt att gat ttt aag gag atg ggc aga atg ggg atc ttt gac       48
Met Asp Phe Ile Asp Phe Lys Glu Met Gly Arg Met Gly Ile Phe Asp         
1               5                   10                  15              

gga aag gtc gca atc att act ggc ggg ggc aag gcc aaa tcg atc ggc       96
Gly Lys Val Ala Ile Ile Thr Gly Gly Gly Lys Ala Lys Ser Ile Gly         
            20                  25                  30                  

tac ggc att gcc gtg gcc tat gct aag gag ggg gcc aac ctg gtc ctg      144
Tyr Gly Ile Ala Val Ala Tyr Ala Lys Glu Gly Ala Asn Leu Val Leu         
        35                  40                  45                      

acc ggc aga aac gag cag aaa ctg ctg gac gcc aag gag gag ctg gag      192
Thr Gly Arg Asn Glu Gln Lys Leu Leu Asp Ala Lys Glu Glu Leu Glu         
    50                  55                  60                          

cgc ctc tac ggc atc aag gtg ttg ccg ctg gcg gtg gac gtc acc ccc      240
Arg Leu Tyr Gly Ile Lys Val Leu Pro Leu Ala Val Asp Val Thr Pro         
65                  70                  75                  80          

agc gat gag tcg gag gac cgg gtc aag gaa gcc gtg cag aag gtc atc      288
Ser Asp Glu Ser Glu Asp Arg Val Lys Glu Ala Val Gln Lys Val Ile         
                85                  90                  95              

gcc gaa ttc ggc cgc atc gac gtg ctg atc aac aac gcc cag gcg tcg      336
Ala Glu Phe Gly Arg Ile Asp Val Leu Ile Asn Asn Ala Gln Ala Ser         
            100                 105                 110                 

gcc tcg ggc atc ccc ctg tcc atg cag acc aaa gac cac ttt gac ctg      384
Ala Ser Gly Ile Pro Leu Ser Met Gln Thr Lys Asp His Phe Asp Leu         
        115                 120                 125                     

ggc atc tac tcc ggg ctc tac gcc acc ttc tac tac atg agg gag tgc      432
Gly Ile Tyr Ser Gly Leu Tyr Ala Thr Phe Tyr Tyr Met Arg Glu Cys         
    130                 135                 140                         

tat ccc tac ctg aag gag acc cag ggc tcg gtc atc aac ttc gcc tcc      480
Tyr Pro Tyr Leu Lys Glu Thr Gln Gly Ser Val Ile Asn Phe Ala Ser         
145                 150                 155                 160         

ggc gcc ggc ctc ttc ggc aac gtg ggt cag tgc tcc tac gcc gcc gcc      528
Gly Ala Gly Leu Phe Gly Asn Val Gly Gln Cys Ser Tyr Ala Ala Ala         
                165                 170                 175             

aaa gag ggc atc cgc ggc ctc tcc cgc gtc gcg gcc acc gag tgg ggc      576
Lys Glu Gly Ile Arg Gly Leu Ser Arg Val Ala Ala Thr Glu Trp Gly         
            180                 185                 190                 

aag gac aac atc aac gtc aac gtg gtc tgc ccc ctg gcc atg acc gcc      624
Lys Asp Asn Ile Asn Val Asn Val Val Cys Pro Leu Ala Met Thr Ala         
        195                 200                 205                     

cag ctg gag aac ttc aag ctc tcc tac cct gag gcc tac gag aaa aac      672
Gln Leu Glu Asn Phe Lys Leu Ser Tyr Pro Glu Ala Tyr Glu Lys Asn         
    210                 215                 220                         

ctc aga ggg gtg ccc atg ggc cgc ttc ggt gac ccc gag ctg gac atc      720
Leu Arg Gly Val Pro Met Gly Arg Phe Gly Asp Pro Glu Leu Asp Ile         
225                 230                 235                 240         

ggc cgg gtc tgc gtg cag ctc ggc tcg ccc gac ttc aag tac atg tcc      768
Gly Arg Val Cys Val Gln Leu Gly Ser Pro Asp Phe Lys Tyr Met Ser         
                245                 250                 255             

ggc gag acc ctc acc ctg gaa ggc ggc atg ggt cag cgc ccc tag          813
Gly Glu Thr Leu Thr Leu Glu Gly Gly Met Gly Gln Arg Pro                 
            260                 265                 270                 


<210>  12
<211>  270
<212>  PRT
<213>  Clostridium sp.

<400>  12

Met Asp Phe Ile Asp Phe Lys Glu Met Gly Arg Met Gly Ile Phe Asp 
1               5                   10                  15      


Gly Lys Val Ala Ile Ile Thr Gly Gly Gly Lys Ala Lys Ser Ile Gly 
            20                  25                  30          


Tyr Gly Ile Ala Val Ala Tyr Ala Lys Glu Gly Ala Asn Leu Val Leu 
        35                  40                  45              


Thr Gly Arg Asn Glu Gln Lys Leu Leu Asp Ala Lys Glu Glu Leu Glu 
    50                  55                  60                  


Arg Leu Tyr Gly Ile Lys Val Leu Pro Leu Ala Val Asp Val Thr Pro 
65                  70                  75                  80  


Ser Asp Glu Ser Glu Asp Arg Val Lys Glu Ala Val Gln Lys Val Ile 
                85                  90                  95      


Ala Glu Phe Gly Arg Ile Asp Val Leu Ile Asn Asn Ala Gln Ala Ser 
            100                 105                 110         


Ala Ser Gly Ile Pro Leu Ser Met Gln Thr Lys Asp His Phe Asp Leu 
        115                 120                 125             


Gly Ile Tyr Ser Gly Leu Tyr Ala Thr Phe Tyr Tyr Met Arg Glu Cys 
    130                 135                 140                 


Tyr Pro Tyr Leu Lys Glu Thr Gln Gly Ser Val Ile Asn Phe Ala Ser 
145                 150                 155                 160 


Gly Ala Gly Leu Phe Gly Asn Val Gly Gln Cys Ser Tyr Ala Ala Ala 
                165                 170                 175     


Lys Glu Gly Ile Arg Gly Leu Ser Arg Val Ala Ala Thr Glu Trp Gly 
            180                 185                 190         


Lys Asp Asn Ile Asn Val Asn Val Val Cys Pro Leu Ala Met Thr Ala 
        195                 200                 205             


Gln Leu Glu Asn Phe Lys Leu Ser Tyr Pro Glu Ala Tyr Glu Lys Asn 
    210                 215                 220                 


Leu Arg Gly Val Pro Met Gly Arg Phe Gly Asp Pro Glu Leu Asp Ile 
225                 230                 235                 240 


Gly Arg Val Cys Val Gln Leu Gly Ser Pro Asp Phe Lys Tyr Met Ser 
                245                 250                 255     


Gly Glu Thr Leu Thr Leu Glu Gly Gly Met Gly Gln Arg Pro 
            260                 265                 270 


<210>  13
<211>  774
<212>  DNA
<213>  Clostridium sp.


<220>
<221>  CDS
<222>  (1)..(774)

<400>  13
atc ttt gac gga aag gtc gca atc att act ggc ggg ggc aag gcc aaa       48
Ile Phe Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Gly Lys Ala Lys         
1               5                   10                  15              

tcg atc ggc tac ggc att gcc gtg gcc tat gct aag gag ggg gcc aac       96
Ser Ile Gly Tyr Gly Ile Ala Val Ala Tyr Ala Lys Glu Gly Ala Asn         
            20                  25                  30                  

ctg gtc ctg acc ggc aga aac gag cag aaa ctg ctg gac gcc aag gag      144
Leu Val Leu Thr Gly Arg Asn Glu Gln Lys Leu Leu Asp Ala Lys Glu         
        35                  40                  45                      

gag ctg gag cgc ctc tac ggc atc aag gtg ttg ccg ctg gcg gtg gac      192
Glu Leu Glu Arg Leu Tyr Gly Ile Lys Val Leu Pro Leu Ala Val Asp         
    50                  55                  60                          

gtc acc ccc agc gat gag tcg gag gac cgg gtc aag gaa gcc gtg cag      240
Val Thr Pro Ser Asp Glu Ser Glu Asp Arg Val Lys Glu Ala Val Gln         
65                  70                  75                  80          

aag gtc atc gcc gaa ttc ggc cgc atc gac gtg ctg atc aac aac gcc      288
Lys Val Ile Ala Glu Phe Gly Arg Ile Asp Val Leu Ile Asn Asn Ala         
                85                  90                  95              

cag gcg tcg gcc tcg ggc atc ccc ctg tcc atg cag acc aaa gac cac      336
Gln Ala Ser Ala Ser Gly Ile Pro Leu Ser Met Gln Thr Lys Asp His         
            100                 105                 110                 

ttt gac ctg ggc atc tac tcc ggg ctc tac gcc acc ttc tac tac atg      384
Phe Asp Leu Gly Ile Tyr Ser Gly Leu Tyr Ala Thr Phe Tyr Tyr Met         
        115                 120                 125                     

agg gag tgc tat ccc tac ctg aag gag acc cag ggc tcg gtc atc aac      432
Arg Glu Cys Tyr Pro Tyr Leu Lys Glu Thr Gln Gly Ser Val Ile Asn         
    130                 135                 140                         

ttc gcc tcc ggc gcc ggc ctc ttc ggc aac gtg ggt cag tgc tcc tac      480
Phe Ala Ser Gly Ala Gly Leu Phe Gly Asn Val Gly Gln Cys Ser Tyr         
145                 150                 155                 160         

gcc gcc gcc aaa gag ggc atc cgc ggc ctc tcc cgc gtc gcg gcc acc      528
Ala Ala Ala Lys Glu Gly Ile Arg Gly Leu Ser Arg Val Ala Ala Thr         
                165                 170                 175             

gag tgg ggc aag gac aac atc aac gtc aac gtg gtc tgc ccc ctg gcc      576
Glu Trp Gly Lys Asp Asn Ile Asn Val Asn Val Val Cys Pro Leu Ala         
            180                 185                 190                 

atg acc gcc cag ctg gag aac ttc aag ctc tcc tac cct gag gcc tac      624
Met Thr Ala Gln Leu Glu Asn Phe Lys Leu Ser Tyr Pro Glu Ala Tyr         
        195                 200                 205                     

gag aaa aac ctc aga ggg gtg ccc atg ggc cgc ttc ggt gac ccc gag      672
Glu Lys Asn Leu Arg Gly Val Pro Met Gly Arg Phe Gly Asp Pro Glu         
    210                 215                 220                         

ctg gac atc ggc cgg gtc tgc gtg cag ctc ggc tcg ccc gac ttc aag      720
Leu Asp Ile Gly Arg Val Cys Val Gln Leu Gly Ser Pro Asp Phe Lys         
225                 230                 235                 240         

tac atg tcc ggc gag acc ctc acc ctg gaa ggc ggc atg ggt cag cgc      768
Tyr Met Ser Gly Glu Thr Leu Thr Leu Glu Gly Gly Met Gly Gln Arg         
                245                 250                 255             

ccc tag                                                              774
Pro                                                                     
                                                                        


<210>  14
<211>  257
<212>  PRT
<213>  Clostridium sp.

<400>  14

Ile Phe Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Gly Lys Ala Lys 
1               5                   10                  15      


Ser Ile Gly Tyr Gly Ile Ala Val Ala Tyr Ala Lys Glu Gly Ala Asn 
            20                  25                  30          


Leu Val Leu Thr Gly Arg Asn Glu Gln Lys Leu Leu Asp Ala Lys Glu 
        35                  40                  45              


Glu Leu Glu Arg Leu Tyr Gly Ile Lys Val Leu Pro Leu Ala Val Asp 
    50                  55                  60                  


Val Thr Pro Ser Asp Glu Ser Glu Asp Arg Val Lys Glu Ala Val Gln 
65                  70                  75                  80  


Lys Val Ile Ala Glu Phe Gly Arg Ile Asp Val Leu Ile Asn Asn Ala 
                85                  90                  95      


Gln Ala Ser Ala Ser Gly Ile Pro Leu Ser Met Gln Thr Lys Asp His 
            100                 105                 110         


Phe Asp Leu Gly Ile Tyr Ser Gly Leu Tyr Ala Thr Phe Tyr Tyr Met 
        115                 120                 125             


Arg Glu Cys Tyr Pro Tyr Leu Lys Glu Thr Gln Gly Ser Val Ile Asn 
    130                 135                 140                 


Phe Ala Ser Gly Ala Gly Leu Phe Gly Asn Val Gly Gln Cys Ser Tyr 
145                 150                 155                 160 


Ala Ala Ala Lys Glu Gly Ile Arg Gly Leu Ser Arg Val Ala Ala Thr 
                165                 170                 175     


Glu Trp Gly Lys Asp Asn Ile Asn Val Asn Val Val Cys Pro Leu Ala 
            180                 185                 190         


Met Thr Ala Gln Leu Glu Asn Phe Lys Leu Ser Tyr Pro Glu Ala Tyr 
        195                 200                 205             


Glu Lys Asn Leu Arg Gly Val Pro Met Gly Arg Phe Gly Asp Pro Glu 
    210                 215                 220                 


Leu Asp Ile Gly Arg Val Cys Val Gln Leu Gly Ser Pro Asp Phe Lys 
225                 230                 235                 240 


Tyr Met Ser Gly Glu Thr Leu Thr Leu Glu Gly Gly Met Gly Gln Arg 
                245                 250                 255     


Pro 
    


<210>  15
<211>  777
<212>  DNA
<213>  Artificial

<220>
<223>  Mutant Q97H


<220>
<221>  CDS
<222>  (1)..(777)

<400>  15
atg atc ttt gac gga aag gtc gca atc att act ggc ggg ggc aag gcc       48
Met Ile Phe Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Gly Lys Ala         
1               5                   10                  15              

aaa tcg atc ggc tac ggc att gcc gtg gcc tat gct aag gag ggg gcc       96
Lys Ser Ile Gly Tyr Gly Ile Ala Val Ala Tyr Ala Lys Glu Gly Ala         
            20                  25                  30                  

aac ctg gtc ctg acc ggc aga aac gag cag aaa ctg ctg gac gcc aag      144
Asn Leu Val Leu Thr Gly Arg Asn Glu Gln Lys Leu Leu Asp Ala Lys         
        35                  40                  45                      

gag gag ctg gag cgc ctc tac ggc atc aag gtg ttg ccg ctg gcg gtg      192
Glu Glu Leu Glu Arg Leu Tyr Gly Ile Lys Val Leu Pro Leu Ala Val         
    50                  55                  60                          

gac gtc acc ccc agc gat gag tcg gag gac cgg gtc aag gaa gcc gtg      240
Asp Val Thr Pro Ser Asp Glu Ser Glu Asp Arg Val Lys Glu Ala Val         
65                  70                  75                  80          

cag aag gtc atc gcc gaa ttc ggc cgc atc gac gtg ctg atc aac aac      288
Gln Lys Val Ile Ala Glu Phe Gly Arg Ile Asp Val Leu Ile Asn Asn         
                85                  90                  95              

gcc cat gcg tcg gcc tcg ggc atc ccc ctg tcc atg cag acc aaa gac      336
Ala His Ala Ser Ala Ser Gly Ile Pro Leu Ser Met Gln Thr Lys Asp         
            100                 105                 110                 

cac ttt gac ctg ggc atc tac tcc ggg ctc tac gcc acc ttc tac tac      384
His Phe Asp Leu Gly Ile Tyr Ser Gly Leu Tyr Ala Thr Phe Tyr Tyr         
        115                 120                 125                     

atg agg gag tgc tat ccc tac ctg aag gag act cag ggc tcg gtc atc      432
Met Arg Glu Cys Tyr Pro Tyr Leu Lys Glu Thr Gln Gly Ser Val Ile         
    130                 135                 140                         

aac ttc gcc tcc ggc gcc ggc ctc ttc ggc aac gtg ggt cag tgc tcc      480
Asn Phe Ala Ser Gly Ala Gly Leu Phe Gly Asn Val Gly Gln Cys Ser         
145                 150                 155                 160         

tac gcc gcc gcc aaa gag ggc atc cgc ggc ctc tcc cgc gtc gcg gcc      528
Tyr Ala Ala Ala Lys Glu Gly Ile Arg Gly Leu Ser Arg Val Ala Ala         
                165                 170                 175             

acc gag tgg ggc aag gac aac atc aac gtc aac gtg gtc tgc ccc ctg      576
Thr Glu Trp Gly Lys Asp Asn Ile Asn Val Asn Val Val Cys Pro Leu         
            180                 185                 190                 

gcc atg acc gcc cag ctg gag aac ttc aag ctc tcc tac cct gag gcc      624
Ala Met Thr Ala Gln Leu Glu Asn Phe Lys Leu Ser Tyr Pro Glu Ala         
        195                 200                 205                     

tac gag aaa aac ctc aga ggg gtg ccc atg ggc cgc ttc ggt gac ccc      672
Tyr Glu Lys Asn Leu Arg Gly Val Pro Met Gly Arg Phe Gly Asp Pro         
    210                 215                 220                         

gag ctg gac atc ggc cgg gtc tgc gtg cag ctc ggc tcg ccc gac ttc      720
Glu Leu Asp Ile Gly Arg Val Cys Val Gln Leu Gly Ser Pro Asp Phe         
225                 230                 235                 240         

aag tac atg tcc ggc gag acc ctc acc ctg gaa ggc ggc atg ggt cag      768
Lys Tyr Met Ser Gly Glu Thr Leu Thr Leu Glu Gly Gly Met Gly Gln         
                245                 250                 255             

cgc ccc tag                                                          777
Arg Pro                                                                 
                                                                        


<210>  16
<211>  258
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  16

Met Ile Phe Asp Gly Lys Val Ala Ile Ile Thr Gly Gly Gly Lys Ala 
1               5                   10                  15      


Lys Ser Ile Gly Tyr Gly Ile Ala Val Ala Tyr Ala Lys Glu Gly Ala 
            20                  25                  30          


Asn Leu Val Leu Thr Gly Arg Asn Glu Gln Lys Leu Leu Asp Ala Lys 
        35                  40                  45              


Glu Glu Leu Glu Arg Leu Tyr Gly Ile Lys Val Leu Pro Leu Ala Val 
    50                  55                  60                  


Asp Val Thr Pro Ser Asp Glu Ser Glu Asp Arg Val Lys Glu Ala Val 
65                  70                  75                  80  


Gln Lys Val Ile Ala Glu Phe Gly Arg Ile Asp Val Leu Ile Asn Asn 
                85                  90                  95      


Ala His Ala Ser Ala Ser Gly Ile Pro Leu Ser Met Gln Thr Lys Asp 
            100                 105                 110         


His Phe Asp Leu Gly Ile Tyr Ser Gly Leu Tyr Ala Thr Phe Tyr Tyr 
        115                 120                 125             


Met Arg Glu Cys Tyr Pro Tyr Leu Lys Glu Thr Gln Gly Ser Val Ile 
    130                 135                 140                 


Asn Phe Ala Ser Gly Ala Gly Leu Phe Gly Asn Val Gly Gln Cys Ser 
145                 150                 155                 160 


Tyr Ala Ala Ala Lys Glu Gly Ile Arg Gly Leu Ser Arg Val Ala Ala 
                165                 170                 175     


Thr Glu Trp Gly Lys Asp Asn Ile Asn Val Asn Val Val Cys Pro Leu 
            180                 185                 190         


Ala Met Thr Ala Gln Leu Glu Asn Phe Lys Leu Ser Tyr Pro Glu Ala 
        195                 200                 205             


Tyr Glu Lys Asn Leu Arg Gly Val Pro Met Gly Arg Phe Gly Asp Pro 
    210                 215                 220                 


Glu Leu Asp Ile Gly Arg Val Cys Val Gln Leu Gly Ser Pro Asp Phe 
225                 230                 235                 240 


Lys Tyr Met Ser Gly Glu Thr Leu Thr Leu Glu Gly Gly Met Gly Gln 
                245                 250                 255     


Arg Pro 
        


<210>  17
<211>  53
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  17
aaaaaagctt ataattatcc ttataggacg tcatggtgcg cccagatagg gtg            53


<210>  18
<211>  60
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  18
cagattgtac aaatgtggtg ataacagata agtcgtcatg tttaacttac ctttctttgt     60


<210>  19
<211>  49
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  19
tgaacgcaag tttctaattt cggtttccta tcgatagagg aaagtgtct                 49


<210>  20
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  20
ttaattgagc tcctgtaccc caccacc                                         27


<210>  21
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  21
gtgtttaatt ctgacaacct gagactcgac                                      30


<210>  22
<211>  1266
<212>  DNA
<213>  Artificial

<220>
<223>  FDH D221G Mutante mit His-Tag


<220>
<221>  CDS
<222>  (1)..(1266)

<400>  22
atg gca aag gtc ctg tgc gtt ctt tac gat gat ccg gtc gac ggc tac       48
Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr         
1               5                   10                  15              

ccg aag acc tat gcc cgc gac gat ctt ccg aag atc gac cac tat ccg       96
Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro         
            20                  25                  30                  

ggc ggc cag atc ttg ccg acg ccg aag gcc atc gac ttc acg ccc ggg      144
Gly Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly         
        35                  40                  45                      

cag ttg ctc ggc tcc gtc tcc ggc gag ctc ggc ctg cgc gaa tat ctc      192
Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu         
    50                  55                  60                          

gaa tcc aac ggc cac acc ctg gtc gtg acc tcc gac aag gac ggc ccc      240
Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro         
65                  70                  75                  80          

gac tcg gtg ttc gag cgc gag ctg gtc gat gcg gat gtc gtc atc tcc      288
Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser         
                85                  90                  95              

cag ccc ttc tgg ccg gcc tat ctg acg ccc gag cgc atc gcc aag gcc      336
Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala         
            100                 105                 110                 

aag aac ctg aag ctc gcg ctc acc gcc ggc atc ggt tcc gac cac gtc      384
Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val         
        115                 120                 125                     

gat ctt cag tcg gct atc gac cgc aac gtc acc gtg gcg gaa gtc acc      432
Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr         
    130                 135                 140                         

tac tgc aac tcg atc agc gtc gcc gag cat gtg gtg atg atg atc ctg      480
Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu         
145                 150                 155                 160         

tcg ctg gtg cgc aac tat ctg ccc tcg cac gaa tgg gcg cgg aag ggc      528
Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly         
                165                 170                 175             

ggc tgg aac atc gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg      576
Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala         
            180                 185                 190                 

atg cat gtc ggc acc gtg gcc gcc ggc cgc atc ggt ctc gcg gtg ctg      624
Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu         
        195                 200                 205                     

cgc cgt ctg gcg ccg ttc gac gtg cac ctg cac tac acc ggc cgt cac      672
Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His         
    210                 215                 220                         

cgc ctg ccg gaa tcg gtc gag aag gag ctc aac ctc acc tgg cac gcg      720
Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala         
225                 230                 235                 240         

acc cgc gag gac atg tat ccg gtt tgc gac gtg gtg acg ctg aac tgc      768
Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys         
                245                 250                 255             

ccg ctg cac ccc gaa acc gag cac atg atc aat gac gag acg ctg aag      816
Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys         
            260                 265                 270                 

ctg ttc aag cgt ggc gcc tac atc gtc aac acc gcc cgc ggc aag ctg      864
Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu         
        275                 280                 285                     

tgc gac cgc gat gcc gtg gca cgt gcg ctc gaa tcc ggc cgg ctg gcc      912
Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala         
    290                 295                 300                         

ggc tat gcc ggc gac gtg tgg ttc ccg cag ccg gcg ccg aag gac cac      960
Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His         
305                 310                 315                 320         

ccc tgg cgg acg atg ccc tat aac ggc atg acc ccg cac atc tcc ggc     1008
Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly         
                325                 330                 335             

acc acg ctg acc gcg cag gcg cgt tat gcg gcg ggc acc cgc gag atc     1056
Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile         
            340                 345                 350                 

ctg gag tgc ttc ttc gag ggc cgt ccg atc cgc gac gaa tac ctc atc     1104
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile         
        355                 360                 365                     

gtg cag ggc ggc gct ctt gcc ggc acc ggc gcg cat tcc tac tcg aag     1152
Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys         
    370                 375                 380                         

ggc aat gcc acc ggc ggt tcg gaa gag gcc gcc aag ttc aag aag gcg     1200
Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala         
385                 390                 395                 400         

gct gag aat tcg agc tcc gtc gac aag ctt gcg gcc gca ctc gag cac     1248
Ala Glu Asn Ser Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His         
                405                 410                 415             

cac cac cac cac cac tga                                             1266
His His His His His                                                     
            420                                                         


<210>  23
<211>  421
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  23

Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 
1               5                   10                  15      


Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 
            20                  25                  30          


Gly Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 
        35                  40                  45              


Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 
    50                  55                  60                  


Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 
65                  70                  75                  80  


Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 
                85                  90                  95      


Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 
            100                 105                 110         


Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val 
        115                 120                 125             


Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr 
    130                 135                 140                 


Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 
145                 150                 155                 160 


Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 
                165                 170                 175     


Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 
            180                 185                 190         


Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 
        195                 200                 205             


Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 
    210                 215                 220                 


Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 
225                 230                 235                 240 


Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 
                245                 250                 255     


Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 
            260                 265                 270         


Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 
        275                 280                 285             


Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 
    290                 295                 300                 


Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 
305                 310                 315                 320 


Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 
                325                 330                 335     


Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 
            340                 345                 350         


Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 
        355                 360                 365             


Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 
    370                 375                 380                 


Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 
385                 390                 395                 400 


Ala Glu Asn Ser Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His 
                405                 410                 415     


His His His His His 
            420     


<210>  24
<211>  792
<212>  DNA
<213>  Collinsella aerofaciens


<220>
<221>  CDS
<222>  (1)..(792)

<400>  24
atg aac ctg agg gag aag tac ggt gag tgg ggc ctg atc ctg ggc gcg       48
Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala         
1               5                   10                  15              

acc gag ggc gtc ggc aag gcg ttc tgc gag aag atc gcc gcc ggc ggc       96
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly         
            20                  25                  30                  

atg aac gtc gtc atg gtc ggc cgt cgc gag gag aag ctg aac gtg ctc      144
Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu         
        35                  40                  45                      

gca ggc gag atc cgc gag acc tac ggc gtg gag acc aag gtc gtg cgc      192
Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg         
    50                  55                  60                          

gcc gac ttt agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc      240
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr         
65                  70                  75                  80          

gag ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc      288
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser         
                85                  90                  95              

ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc      336
Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile         
            100                 105                 110                 

aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac atg cgg      384
Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg         
        115                 120                 125                     

atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac gtc tcg tcg atg      432
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met         
    130                 135                 140                         

acc ggc atc agc tcc agc ccc tgg aac ggc cag tac ggc gcg ggc aag      480
Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys         
145                 150                 155                 160         

gcc ttc atc ctc aag atg acc gag gcc gtg gcc tgc gag tgc gag ggc      528
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly         
                165                 170                 175             

acc ggc gtc gac gtc gag gtc atc acc ctc ggc acc acc cta acc ccc      576
Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro         
            180                 185                 190                 

agc ctg ctg tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg      624
Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met         
        195                 200                 205                     

aag atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag      672
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys         
    210                 215                 220                         

ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc      720
Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser         
225                 230                 235                 240         

gtc cac gac tgg aag gca aac cac acc gag gac gag tac atc cgc tac      768
Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr         
                245                 250                 255             

atg ggg tcg ttc tac cgc gac tag                                      792
Met Gly Ser Phe Tyr Arg Asp                                             
            260                                                         


<210>  25
<211>  263
<212>  PRT
<213>  Collinsella aerofaciens

<400>  25

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  26
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  PCR Primer

<400>  26
gggaattcca tatgaacctg agggagaagt a                                    31


<210>  27
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  27
cccaagcttc tagtcgcggt agaacga                                         27


<210>  28
<211>  774
<212>  DNA
<213>  Comamonas testosteroni


<220>
<221>  CDS
<222>  (1)..(774)

<400>  28
atg tcc atc atc gtg ata agc ggc tgc gcc acc ggc att ggt gcc gct       48
Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr Gly Ile Gly Ala Ala         
1               5                   10                  15              

acg cgc aag gtc ctg gag gcg gcc ggt cac cag atc gta ggc atc gat       96
Thr Arg Lys Val Leu Glu Ala Ala Gly His Gln Ile Val Gly Ile Asp         
            20                  25                  30                  

ata cgc gat gcg gaa gtg att gcc gat ctc tcg acg gcc gaa ggt cga      144
Ile Arg Asp Ala Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg         
        35                  40                  45                      

aag cag gcg att gcc gat gta ctg gcg aag tgc agc aag ggc atg gac      192
Lys Gln Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp         
    50                  55                  60                          

ggc ctg gtg ctg tgc gcc ggc ctg gga ccg cag acc aag gtg ctt ggc      240
Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly         
65                  70                  75                  80          

aat gtg gtt tcg gtc aat tat ttt ggc gcg acc gag ctg atg gat gcc      288
Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala         
                85                  90                  95              

ttt ttg cca gcg ctg aaa aaa ggc cat cag ccc gca gcc gtc gtc atc      336
Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile         
            100                 105                 110                 

tcg tcc gtg gct tcc gcg cat ctg gct ttt gac aag aac cca ctg gcg      384
Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro Leu Ala         
        115                 120                 125                     

ctg gca ctg gaa gcc ggc gag gaa gcc aag gcc cgc gcc att gtc gaa      432
Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg Ala Ile Val Glu         
    130                 135                 140                         

cat gcg gga gag cag ggc gga aat ctg gcc tat gcg ggc agc aag aat      480
His Ala Gly Glu Gln Gly Gly Asn Leu Ala Tyr Ala Gly Ser Lys Asn         
145                 150                 155                 160         

gct ttg acg gtg gct gtg cgc aaa cgc gcc gcc gcc tgg ggc gag gct      528
Ala Leu Thr Val Ala Val Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala         
                165                 170                 175             

ggc gtg cgc ctg aac acc atc gcc ccc ggt gca acc gag act ccc ttg      576
Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu         
            180                 185                 190                 

ctg cag gcg ggc ctg cag gac ccg cgc tat ggc gaa tcc att gcc aag      624
Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys         
        195                 200                 205                     

ttc gtt cct ccc atg ggc cgc cgt gcc gag ccg tcc gag atg gcg tcg      672
Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser         
    210                 215                 220                         

gtc atc gcc ttt ttg atg agc ccg gcc gca agc tat gtg cat ggc gcg      720
Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala         
225                 230                 235                 240         

cag atc gtc att gat ggc ggc att gat gcg gtg atg cgc ccg aca cag      768
Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg Pro Thr Gln         
                245                 250                 255             

ttc tga                                                              774
Phe                                                                     
                                                                        


<210>  29
<211>  257
<212>  PRT
<213>  Comamonas testosteroni

<400>  29

Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr Gly Ile Gly Ala Ala 
1               5                   10                  15      


Thr Arg Lys Val Leu Glu Ala Ala Gly His Gln Ile Val Gly Ile Asp 
            20                  25                  30          


Ile Arg Asp Ala Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg 
        35                  40                  45              


Lys Gln Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp 
    50                  55                  60                  


Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly 
65                  70                  75                  80  


Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala 
                85                  90                  95      


Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile 
            100                 105                 110         


Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro Leu Ala 
        115                 120                 125             


Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg Ala Ile Val Glu 
    130                 135                 140                 


His Ala Gly Glu Gln Gly Gly Asn Leu Ala Tyr Ala Gly Ser Lys Asn 
145                 150                 155                 160 


Ala Leu Thr Val Ala Val Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala 
                165                 170                 175     


Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu 
            180                 185                 190         


Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys 
        195                 200                 205             


Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser 
    210                 215                 220                 


Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala 
225                 230                 235                 240 


Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg Pro Thr Gln 
                245                 250                 255     


Phe 
    


<210>  30
<211>  969
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  CDS
<222>  (1)..(969)

<400>  30
atg gat tcc ata tct ctg cgt gta gca cta aat gat ggt aac ttc att       48
Met Asp Ser Ile Ser Leu Arg Val Ala Leu Asn Asp Gly Asn Phe Ile         
1               5                   10                  15              

cct gta ctg ggg ttt gga acc act gtg cct gag aag gtt gct aag gat       96
Pro Val Leu Gly Phe Gly Thr Thr Val Pro Glu Lys Val Ala Lys Asp         
            20                  25                  30                  

gaa gtt atc aag gct act aaa ata gct ata gat aat gga ttc cgc cat      144
Glu Val Ile Lys Ala Thr Lys Ile Ala Ile Asp Asn Gly Phe Arg His         
        35                  40                  45                      

ttt gac tct gct tat ttg tac gaa gta gaa gag gaa gtg ggc caa gcc      192
Phe Asp Ser Ala Tyr Leu Tyr Glu Val Glu Glu Glu Val Gly Gln Ala         
    50                  55                  60                          

att aga agc aag att gaa gac ggc act gtg aag aga gaa gat ata ttc      240
Ile Arg Ser Lys Ile Glu Asp Gly Thr Val Lys Arg Glu Asp Ile Phe         
65                  70                  75                  80          

tat act tca aag ctt tgg agc act ttc cat aga cca gag ctg gtc cga      288
Tyr Thr Ser Lys Leu Trp Ser Thr Phe His Arg Pro Glu Leu Val Arg         
                85                  90                  95              

act tgc ttg gaa aag aca ctg aaa agc act caa ctg gac tat gtg gat      336
Thr Cys Leu Glu Lys Thr Leu Lys Ser Thr Gln Leu Asp Tyr Val Asp         
            100                 105                 110                 

ctt tat att att cat ttc cca atg gct ttg cag cct gga gat ata ttt      384
Leu Tyr Ile Ile His Phe Pro Met Ala Leu Gln Pro Gly Asp Ile Phe         
        115                 120                 125                     

ttc cca cga gat gag cat gga aaa cta ttg ttt gaa aca gtg gat atc      432
Phe Pro Arg Asp Glu His Gly Lys Leu Leu Phe Glu Thr Val Asp Ile         
    130                 135                 140                         

tgt gac aca tgg gag gcc atg gaa aag tgt aag gat gca gga ttg gcc      480
Cys Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp Ala Gly Leu Ala         
145                 150                 155                 160         

aag tct att ggg gtg tcc aac ttt aac tgc agg cag ctg gag agg att      528
Lys Ser Ile Gly Val Ser Asn Phe Asn Cys Arg Gln Leu Glu Arg Ile         
                165                 170                 175             

ctg aat aag cca ggg ctc aaa tac aag cct gtg tgc aac cag gtg gaa      576
Leu Asn Lys Pro Gly Leu Lys Tyr Lys Pro Val Cys Asn Gln Val Glu         
            180                 185                 190                 

tgt cac ctt tat ctc aac cag agc aaa atg ctg gac tat tgt aag tca      624
Cys His Leu Tyr Leu Asn Gln Ser Lys Met Leu Asp Tyr Cys Lys Ser         
        195                 200                 205                     

aaa gac atc att ctg gtt tcc tac tgc acg ctg gga agt tca cga gac      672
Lys Asp Ile Ile Leu Val Ser Tyr Cys Thr Leu Gly Ser Ser Arg Asp         
    210                 215                 220                         

aaa aca tgg gtg gat cag aaa agt cca gtt ctc cta gat gat cca gtt      720
Lys Thr Trp Val Asp Gln Lys Ser Pro Val Leu Leu Asp Asp Pro Val         
225                 230                 235                 240         

ctt tgt gcc ata gca aag aag tac aag caa acc cca gcc cta gtt gcc      768
Leu Cys Ala Ile Ala Lys Lys Tyr Lys Gln Thr Pro Ala Leu Val Ala         
                245                 250                 255             

ctt cgc tac cag ctg cag cgt ggg gtt gtg ccc ctg atc agg agt ttc      816
Leu Arg Tyr Gln Leu Gln Arg Gly Val Val Pro Leu Ile Arg Ser Phe         
            260                 265                 270                 

aac gcg aag cgg atc aaa gag cta aca cag gtt ttt gaa ttc cag ttg      864
Asn Ala Lys Arg Ile Lys Glu Leu Thr Gln Val Phe Glu Phe Gln Leu         
        275                 280                 285                     

gct tca gag gac atg aaa gcc ctg gat ggc ttg aac aga aat ttc aga      912
Ala Ser Glu Asp Met Lys Ala Leu Asp Gly Leu Asn Arg Asn Phe Arg         
    290                 295                 300                         

tac aac aat gca aaa tat ttt gat gac cat ccc aat cat cca ttt act      960
Tyr Asn Asn Ala Lys Tyr Phe Asp Asp His Pro Asn His Pro Phe Thr         
305                 310                 315                 320         

gat gaa tag                                                          969
Asp Glu                                                                 
                                                                        


<210>  31
<211>  322
<212>  PRT
<213>  Rattus norvegicus

<400>  31

Met Asp Ser Ile Ser Leu Arg Val Ala Leu Asn Asp Gly Asn Phe Ile 
1               5                   10                  15      


Pro Val Leu Gly Phe Gly Thr Thr Val Pro Glu Lys Val Ala Lys Asp 
            20                  25                  30          


Glu Val Ile Lys Ala Thr Lys Ile Ala Ile Asp Asn Gly Phe Arg His 
        35                  40                  45              


Phe Asp Ser Ala Tyr Leu Tyr Glu Val Glu Glu Glu Val Gly Gln Ala 
    50                  55                  60                  


Ile Arg Ser Lys Ile Glu Asp Gly Thr Val Lys Arg Glu Asp Ile Phe 
65                  70                  75                  80  


Tyr Thr Ser Lys Leu Trp Ser Thr Phe His Arg Pro Glu Leu Val Arg 
                85                  90                  95      


Thr Cys Leu Glu Lys Thr Leu Lys Ser Thr Gln Leu Asp Tyr Val Asp 
            100                 105                 110         


Leu Tyr Ile Ile His Phe Pro Met Ala Leu Gln Pro Gly Asp Ile Phe 
        115                 120                 125             


Phe Pro Arg Asp Glu His Gly Lys Leu Leu Phe Glu Thr Val Asp Ile 
    130                 135                 140                 


Cys Asp Thr Trp Glu Ala Met Glu Lys Cys Lys Asp Ala Gly Leu Ala 
145                 150                 155                 160 


Lys Ser Ile Gly Val Ser Asn Phe Asn Cys Arg Gln Leu Glu Arg Ile 
                165                 170                 175     


Leu Asn Lys Pro Gly Leu Lys Tyr Lys Pro Val Cys Asn Gln Val Glu 
            180                 185                 190         


Cys His Leu Tyr Leu Asn Gln Ser Lys Met Leu Asp Tyr Cys Lys Ser 
        195                 200                 205             


Lys Asp Ile Ile Leu Val Ser Tyr Cys Thr Leu Gly Ser Ser Arg Asp 
    210                 215                 220                 


Lys Thr Trp Val Asp Gln Lys Ser Pro Val Leu Leu Asp Asp Pro Val 
225                 230                 235                 240 


Leu Cys Ala Ile Ala Lys Lys Tyr Lys Gln Thr Pro Ala Leu Val Ala 
                245                 250                 255     


Leu Arg Tyr Gln Leu Gln Arg Gly Val Val Pro Leu Ile Arg Ser Phe 
            260                 265                 270         


Asn Ala Lys Arg Ile Lys Glu Leu Thr Gln Val Phe Glu Phe Gln Leu 
        275                 280                 285             


Ala Ser Glu Asp Met Lys Ala Leu Asp Gly Leu Asn Arg Asn Phe Arg 
    290                 295                 300                 


Tyr Asn Asn Ala Lys Tyr Phe Asp Asp His Pro Asn His Pro Phe Thr 
305                 310                 315                 320 


Asp Glu 
        


<210>  32
<211>  401
<212>  PRT
<213>  Mycobacterium vaccae

<400>  32

Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 
1               5                   10                  15      


Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 
            20                  25                  30          


Gly Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 
        35                  40                  45              


Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 
    50                  55                  60                  


Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 
65                  70                  75                  80  


Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 
                85                  90                  95      


Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 
            100                 105                 110         


Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val 
        115                 120                 125             


Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr 
    130                 135                 140                 


Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 
145                 150                 155                 160 


Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 
                165                 170                 175     


Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 
            180                 185                 190         


Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 
        195                 200                 205             


Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Asp Arg His 
    210                 215                 220                 


Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 
225                 230                 235                 240 


Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 
                245                 250                 255     


Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 
            260                 265                 270         


Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 
        275                 280                 285             


Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 
    290                 295                 300                 


Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 
305                 310                 315                 320 


Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 
                325                 330                 335     


Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 
            340                 345                 350         


Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 
        355                 360                 365             


Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 
    370                 375                 380                 


Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 
385                 390                 395                 400 


Val 
    


<210>  33
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  33
cgatcatatg gcaaaggtcc tgtgcgttc                                       29


<210>  34
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  34
gctagaattc tcagccgcct tcttgaact                                       29


<210>  35
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  35
cctgcactac accggccgtc accgcctgc                                       29


<210>  36
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Primer

<400>  36
gctcgaattc tcagaccgcc ttc                                             23


<210>  37
<211>  1206
<212>  DNA
<213>  Artificial

<220>
<223>  Formiate Deydrogenase Mutant


<220>
<221>  CDS
<222>  (1)..(1206)

<400>  37
atg gca aag gtc ctg tgc gtt ctt tac gat gat ccg gtc gac ggc tac       48
Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr         
1               5                   10                  15              

ccg aag acc tat gcc cgc gac gat ctt ccg aag atc gac cac tat ccg       96
Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro         
            20                  25                  30                  

ggc ggc cag atc ttg ccg acg ccg aag gcc atc gac ttc acg ccc ggg      144
Gly Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly         
        35                  40                  45                      

cag ttg ctc ggc tcc gtc tcc ggc gag ctc ggc ctg cgc gaa tat ctc      192
Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu         
    50                  55                  60                          

gaa tcc aac ggc cac acc ctg gtc gtg acc tcc gac aag gac ggc ccc      240
Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro         
65                  70                  75                  80          

gac tcg gtg ttc gag cgc gag ctg gtc gat gcg gat gtc gtc atc tcc      288
Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser         
                85                  90                  95              

cag ccc ttc tgg ccg gcc tat ctg acg ccc gag cgc atc gcc aag gcc      336
Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala         
            100                 105                 110                 

aag aac ctg aag ctc gcg ctc acc gcc ggc atc ggt tcc gac cac gtc      384
Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val         
        115                 120                 125                     

gat ctt cag tcg gct atc gac cgc aac gtc acc gtg gcg gaa gtc acc      432
Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr         
    130                 135                 140                         

tac tgc aac tcg atc agc gtc gcc gag cat gtg gtg atg atg atc ctg      480
Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu         
145                 150                 155                 160         

tcg ctg gtg cgc aac tat ctg ccc tcg cac gaa tgg gcg cgg aag ggc      528
Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly         
                165                 170                 175             

ggc tgg aac atc gcc gac tgc gtc tcc cac gcc tac gac ctc gag gcg      576
Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala         
            180                 185                 190                 

atg cat gtc ggc acc gtg gcc gcc ggc cgc atc ggt ctc gcg gtg ctg      624
Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu         
        195                 200                 205                     

cgc cgt ctg gcg ccg ttc gac gtg cac ctg cac tac acc ggc cgt cac      672
Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His         
    210                 215                 220                         

cgc ctg ccg gaa tcg gtc gag aag gag ctc aac ctc acc tgg cac gcg      720
Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala         
225                 230                 235                 240         

acc cgc gag gac atg tat ccg gtt tgc gac gtg gtg acg ctg aac tgc      768
Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys         
                245                 250                 255             

ccg ctg cac ccc gaa acc gag cac atg atc aat gac gag acg ctg aag      816
Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys         
            260                 265                 270                 

ctg ttc aag cgt ggc gcc tac atc gtc aac acc gcc cgc ggc aag ctg      864
Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu         
        275                 280                 285                     

tgc gac cgc gat gcc gtg gca cgt gcg ctc gaa tcc ggc cgg ctg gcc      912
Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala         
    290                 295                 300                         

ggc tat gcc ggc gac gtg tgg ttc ccg cag ccg gcg ccg aag gac cac      960
Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His         
305                 310                 315                 320         

ccc tgg cgg acg atg ccc tat aac ggc atg acc ccg cac atc tcc ggc     1008
Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly         
                325                 330                 335             

acc acg ctg acc gcg cag gcg cgt tat gcg gcg ggc acc cgc gag atc     1056
Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile         
            340                 345                 350                 

ctg gag tgc ttc ttc gag ggc cgt ccg atc cgc gac gaa tac ctc atc     1104
Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile         
        355                 360                 365                     

gtg cag ggc ggc gct ctt gcc ggc acc ggc gcg cat tcc tac tcg aag     1152
Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys         
    370                 375                 380                         

ggc aat gcc acc ggc ggt tcg gaa gag gcc gcc aag ttc aag aag gcg     1200
Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala         
385                 390                 395                 400         

gtc tga                                                             1206
Val                                                                     
                                                                        


<210>  38
<211>  401
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  38

Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 
1               5                   10                  15      


Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 
            20                  25                  30          


Gly Gly Gln Ile Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 
        35                  40                  45              


Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Glu Tyr Leu 
    50                  55                  60                  


Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 
65                  70                  75                  80  


Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 
                85                  90                  95      


Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 
            100                 105                 110         


Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val 
        115                 120                 125             


Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr 
    130                 135                 140                 


Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 
145                 150                 155                 160 


Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 
                165                 170                 175     


Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 
            180                 185                 190         


Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 
        195                 200                 205             


Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Gly Arg His 
    210                 215                 220                 


Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 
225                 230                 235                 240 


Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 
                245                 250                 255     


Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 
            260                 265                 270         


Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 
        275                 280                 285             


Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 
    290                 295                 300                 


Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 
305                 310                 315                 320 


Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 
                325                 330                 335     


Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 
            340                 345                 350         


Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 
        355                 360                 365             


Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 
    370                 375                 380                 


Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 
385                 390                 395                 400 


Val 
    


