                         SEQUENCE LISTING

<110>  VIB VZW
       VRIJE UNIVERSITEIT BRUSSEL
 
<120>  EPITOPE TAG FOR AFFINITY-BASED APPLICATIONS

<130>  JST/NANO/342

<150>  GB 1008682.5
<151>  2010-05-25

<160>  81    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope tag

<400>  1

Glu Pro Glu Ala 
1               


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  2

Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
1               5                   


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  alfa-synuclein

<400>  3

Asp Tyr Glu Pro Glu Ala 
1               5       


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  4

Tyr Gln Asp Tyr Glu Pro Glu Ala 
1               5               


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  5

Gln Asp Tyr Glu Pro Glu Ala 
1               5           


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  6

Gly Tyr Gln Asp Tyr Glu Pro Glu 
1               5               


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  14N 12-residue peptide

<400>  7

Ser Glu Glu Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
1               5                   10          


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  8

Gly Ala Ala Glu Pro Glu Ala 
1               5           


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  9

Gly Ala Gly Ala Glu Pro Glu Ala 
1               5               


<210>  10
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  10

Gly Ala Gly Ala Gly Tyr Gln Asp Tyr Glu Pro Glu Ala 
1               5                   10              


<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide table 5

<400>  11

Tyr Glu Pro Glu Ala 
1               5   


<210>  12
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Short linker sequence

<400>  12

Gly Ala Gly Ala 
1               


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker sequence

<400>  13

Glu Glu Gly Glu Pro Lys 
1               5       


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker sequence

<400>  14

Glu Glu Ala Glu Pro Lys 
1               5       


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protease cleavage site

<400>  15

Ile Glu Gly Arg 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Thrombin cleavage site

<400>  16

Leu Val Pro Arg 
1               


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Enteroskinase cleaving site

<400>  17

Asp Asp Asp Asp Lys 
1               5   


<210>  18
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_85

<400>  18

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Tyr Ser Asn Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Val Val 
        35                  40                  45              


Ala Arg Ile Ser Thr Arg Gly Ile Lys Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Asn Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Val Ile Tyr Pro Gly Tyr Gly Asp Ser Cys Pro Trp Thr Thr 
            100                 105                 110         


Ser Val Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  19
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_88

<400>  19

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Tyr Ser Arg Ile 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Val Val 
        35                  40                  45              


Ala Arg Ile Ser Thr Arg Gly Ile Lys Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Asn Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Val Ile Tyr Pro Gly Tyr Gly Asp Ser Cys Pro Trp Thr Thr 
            100                 105                 110         


Ser Val Asn Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  20
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_Syn2 PstI

<400>  20

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Ser Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Leu Gly Gly Val Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Phe Ser Pro Gly Tyr Cys Gly Gly Ser Trp Ser Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  21
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_Syn2

<400>  21

Gln Gly Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Ser Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Leu Gly Gly Val Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Phe Ser Pro Gly Tyr Cys Gly Gly Ser Trp Ser Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  22
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_Syn1b

<400>  22

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Pro Ser Gly Leu Asn Ala Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ser Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Asn Ala Gly Ile Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Leu Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Ser Ala Met Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Ser Ser Pro Gly Tyr Cys Gly Gly Asn Trp Asp Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  23
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb_Syn1c

<400>  23

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Pro Leu Gly Leu Asn Ala Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ser Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Asn Ala Gly Ile Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Leu Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Ser Ala Met Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Ser Ser Pro Gly Tyr Cys Gly Gly Asn Trp Asp Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  24
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb_Syn1a

<400>  24

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Ser Leu Ala Cys Ala Pro Ser Gly Leu Asn Ala Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Ser Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Asn Ala Gly Ile Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Leu Ser Arg Asp Asn Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Ser Ala Ile Tyr Phe 
                85                  90                  95      


Cys Ala Ala Lys Ser Ser Pro Gly Tyr Cys Gly Gly Asn Trp Asp Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  25
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb_86

<400>  25

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Leu Ser Gly Tyr Thr Phe Arg Gly Asn 
            20                  25                  30          


Arg Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Thr Gly Gly Val Asn Thr Tyr Val Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Gln Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Gln Pro Glu Asp Thr Ala Met Tyr Phe Cys 
                85                  90                  95      


Ala Ala Asp Leu Thr Gly Trp Arg Pro Val Gly Phe Ser Gly Tyr Asn 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  26
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb_87

<400>  26

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Tyr Ser Gly Tyr Met Ala 
            20                  25                  30          


Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Ile Ala Ala Ile 
        35                  40                  45              


Tyr Arg Gly Asp Lys Ile Thr Tyr Tyr Ala His Ser Val Gln Gly Arg 
    50                  55                  60                  


Phe Thr Ile Ser Gln Ala Asn Ala Lys Asn Thr Val Tyr Leu Leu Met 
65                  70                  75                  80  


Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Ala Arg 
                85                  90                  95      


Arg Val Val Ala Asp Ser Pro Leu Leu Ser Lys Thr Tyr Ala Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  27

Gly Leu Thr Tyr Ser Asn Tyr Cys 
1               5               


<210>  28
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  28

Ile Ser Thr Arg Gly Ile Lys Thr 
1               5               


<210>  29
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  29

Ala Ala Val Ile Tyr Pro Gly Tyr Gly Asp Ser Cys Pro Trp Thr Thr 
1               5                   10                  15      


Ser Val Asn Tyr 
            20  


<210>  30
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  30

Gly Tyr Thr Tyr Ser Arg Ile Cys 
1               5               


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  31

Gly Ile Asp Ser Ser Ser Tyr Cys 
1               5               


<210>  32
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  32

Ile Asn Gly Leu Gly Gly Val Lys Thr 
1               5                   


<210>  33
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  33

Ala Ala Lys Phe Ser Pro Gly Tyr Cys Gly Gly Ser Trp Ser Asn Phe 
1               5                   10                  15      


Gly Tyr 
        


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  34

Gly Leu Asn Ala Ser Ser Tyr Cys 
1               5               


<210>  35
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  35

Ile Asn Gly Asn Ala Gly Ile Lys Thr 
1               5                   


<210>  36
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  36

Ala Ala Lys Ser Ser Pro Gly Tyr Cys Gly Gly Asn Trp Asp Asn Phe 
1               5                   10                  15      


Gly Tyr 
        


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  37

Gly Tyr Thr Phe Arg Gly Asn Arg 
1               5               


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  38

Ile Asn Thr Gly Gly Val Asn Thr 
1               5               


<210>  39
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  39

Ala Ala Asp Leu Thr Gly Trp Arg Pro Val Gly Phe Ser Gly Tyr Asn 
1               5                   10                  15      


Tyr 
    


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  40

Gly Tyr Ser Gly Tyr 
1               5   


<210>  41
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  41

Ile Tyr Arg Gly Asp Lys Ile Thr 
1               5               


<210>  42
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR

<400>  42

Ala Ala Arg Arg Val Val Ala Asp Ser Pro Leu Leu Ser Lys Thr Tyr 
1               5                   10                  15      


Ala Tyr 
        


<210>  43
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  43
gatgtgcagc tgcaggagtc tggrggagg                                         29


<210>  44
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  44
gtagcggccg ctggggtctt cggggtggtg cgctgaggag acggtgacct gggt             54


<210>  45
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
gtagcggccg cttggttggg gtatcttggg ttctgaggag acggtgacct gggt             54


<210>  46
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
gtagcggccg cttacttcat tcgttcctga ggagacggtg acctgggt                    48


<210>  47
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
gtcaccgtct cctcacacca ccatcaccat cacgaacctg aagcctagta ccc              53


<210>  48
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
gtacgggtac taggcttcag gttcgtgatg gtgatggtgg tgtgaggaga cg               52


<210>  49
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
ttcgaattca ttacgcttcc ggttcataat cctgatatcc tttgtagagc tcatccatgc       60


<210>  50
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  50
ttcgaattca ttacgcttcc ggttcataat cctgatattt gtagagctca tccatgcc         58


<210>  51
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  51
ttcgaattca ttacgcttcc ggttcataat cctgtttgta gagctcatcc atgcc            55


<210>  52
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  52
ttcgaattca ttattccggt tcataatcct g                                      31


<210>  53
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  53
acccaggtca ccgtctcctc agggtatcaa gactacgaac ctgaagccta gtacccgtac       60

gacgt                                                                   65


<210>  54
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  54
cgccagggtt ttcccagtca cgac                                              24


<210>  55
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  55
acccaggtca ccgtctcctc aggggcagcg gaacctgaag cctagtaccc gtacgacgtt       60

c                                                                       61


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  56
ttatgcttcc ggctcgtatg                                                   20


<210>  57
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  57
ccacagacag ccctcatag                                                    19


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  58
tgaggagacg gtgacctggg t                                                 21


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  59
attaatacga ctcactatag g                                                 21


<210>  60
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  60
tcgagcggcc gcttaggctt caggttcgta gtcttgatac cctgaagctc ctgcaccatt       60

ctc                                                                     63


<210>  61
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  61
tcgagcggcc gcttaggctt caggttcagc tcctgcacca ttctc                       45


<210>  62
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  62
gcttggtacc tcatgagcaa aggagaagaa c                                      31


<210>  63
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  63
ttcgaattca ttacgcttcc ggttcataat cctgatatcc tttgtagagc tcatccatgc       60


<210>  64
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  64
ttcgaattca ttacgcttcc ggttctgcgg ctcctttgta gagctcatcc atgcc            55


<210>  65
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_196

<400>  65

Gln Val Gln Leu Gln Val Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Val Ser Asn Ser 
            20                  25                  30          


Arg Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Arg Pro Ser Gly Glu Val Ile Tyr Ala Thr Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu His 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Arg Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Arg Pro Ser Gly Val Arg Gly Gln Gly Thr Gln Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  66
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody Nb_458

<400>  66

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Val Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Pro Gly Leu Ser Phe Ser Asn Tyr 
            20                  25                  30          


Ile Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Ala Arg Glu Phe Val 
        35                  40                  45              


Ala Ala Ile Ser Trp Asn Gly Gly Glu Thr Asn Tyr Val Asp Ser Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Asp Tyr Arg Ser Phe Ser Asn Pro Asp Ser Asp Pro Ser Arg 
            100                 105                 110         


Tyr Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  67
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  67
taatcatgag caaaggagaa gaacttttca c                                      31


<210>  68
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanobody biNbSyn2

<400>  68

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ile Asp Ser Ser Ser Tyr 
            20                  25                  30          


Cys Met Gly Trp Phe Arg Gln Arg Pro Gly Lys Glu Arg Glu Gly Val 
        35                  40                  45              


Ala Arg Ile Asn Gly Leu Gly Gly Val Lys Thr Ala Tyr Ala Asp Ser 
    50                  55                  60                  


Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Glu Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ile Tyr Tyr 
                85                  90                  95      


Cys Ala Ala Lys Phe Ser Pro Gly Tyr Cys Gly Gly Ser Trp Ser Asn 
            100                 105                 110         


Phe Gly Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Ser Gln Val Gln Leu Gln Glu Ser Gly Gly 
    130                 135                 140                 


Gly Ser Val Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
145                 150                 155                 160 


Gly Ile Asp Ser Ser Ser Tyr Cys Met Gly Trp Phe Arg Gln Arg Pro 
                165                 170                 175     


Gly Lys Glu Arg Glu Gly Val Ala Arg Ile Asn Gly Leu Gly Gly Val 
            180                 185                 190         


Lys Thr Ala Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg 
        195                 200                 205             


Asp Asn Ala Glu Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro 
    210                 215                 220                 


Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Ala Lys Phe Ser Pro Gly Tyr 
225                 230                 235                 240 


Cys Gly Gly Ser Trp Ser Asn Phe Gly Tyr Trp Gly Gln Gly Thr Gln 
                245                 250                 255     


Val Thr Val Ser Ser His His His His His His 
            260                 265         


<210>  69
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  69

Gly Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   


<210>  70
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tag

<400>  70

Gly Gly Glu Pro Glu Ala 
1               5       


<210>  71
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  71

Ser Glu Pro Glu Ala 
1               5   


<210>  72
<211>  283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EPEA tagged CsgG

<400>  72

Met Gln Arg Leu Phe Leu Leu Val Ala Val Met Leu Leu Ser Gly Cys 
1               5                   10                  15      


Leu Thr Ala Pro Pro Lys Glu Ala Ala Arg Pro Thr Leu Met Pro Arg 
            20                  25                  30          


Ala Gln Ser Tyr Lys Asp Leu Thr His Leu Pro Ala Pro Thr Gly Lys 
        35                  40                  45              


Ile Phe Val Ser Val Tyr Asn Ile Gln Asp Glu Thr Gly Gln Phe Lys 
    50                  55                  60                  


Pro Tyr Pro Ala Ser Asn Phe Ser Thr Ala Val Pro Gln Ser Ala Thr 
65                  70                  75                  80  


Ala Met Leu Val Thr Ala Leu Lys Asp Ser Arg Trp Phe Ile Pro Leu 
                85                  90                  95      


Glu Arg Gln Gly Leu Gln Asn Leu Leu Asn Glu Arg Lys Ile Ile Arg 
            100                 105                 110         


Ala Ala Gln Glu Asn Gly Thr Val Ala Ile Asn Asn Arg Ile Pro Leu 
        115                 120                 125             


Gln Ser Leu Thr Ala Ala Asn Ile Met Val Glu Gly Ser Ile Ile Gly 
    130                 135                 140                 


Tyr Glu Ser Asn Val Lys Ser Gly Gly Val Gly Ala Arg Tyr Phe Gly 
145                 150                 155                 160 


Ile Gly Ala Asp Thr Gln Tyr Gln Leu Asp Gln Ile Ala Val Asn Leu 
                165                 170                 175     


Arg Val Val Asn Val Ser Thr Gly Glu Ile Leu Ser Ser Val Asn Thr 
            180                 185                 190         


Ser Lys Thr Ile Leu Ser Tyr Glu Val Gln Ala Gly Val Phe Arg Phe 
        195                 200                 205             


Ile Asp Tyr Gln Arg Leu Leu Glu Gly Glu Val Gly Tyr Thr Ser Asn 
    210                 215                 220                 


Glu Pro Val Met Leu Cys Leu Met Ser Ala Ile Glu Thr Gly Val Ile 
225                 230                 235                 240 


Phe Leu Ile Asn Asp Gly Ile Asp Arg Gly Leu Trp Asp Leu Gln Asn 
                245                 250                 255     


Lys Ala Glu Arg Gln Asn Asp Ile Leu Val Lys Tyr Arg His Met Ser 
            260                 265                 270         


Val Pro Pro Glu Ser Gly Gly Glu Pro Glu Ala 
        275                 280             


<210>  73
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  73
cgcggcccag ccggccatgg ctgatgtgca gctggtggag tctgg                       45


<210>  74
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  74
agtaggatcc gccacctcct gaggagaccg tgacctgggt                             40


<210>  75
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  75
tcttggatcc ggcggaggta gtcaggtgca gctgcaggag tctgggggag g                51


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  76
tgaggagacg gtgacctggg t                                                 21


<210>  77
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  77
ggcggcgaac cggaagcgtg actgcaggca tgcaagcttg gct                         43


<210>  78
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  78
tgactgcagg catgcaagct tggct                                             25


<210>  79
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  79
cgcttccggt tcgccgccat ggtgatggtg atggtgggat tcc                         43


<210>  80
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  80
atggtgatgg tgatggtggg attcc                                             25


<210>  81
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  81

Gly Gly Gly Gly Ser 
1               5   


