                         SEQUENCE LISTING

<110>  MASSACHUSETTS INSTITUTE OF TECHNOLOGY
       THE RESEARCH FOUDNATION OF STATE UNIVERSITY OF NEW YORK
       STEPHANOPOULOS, Gregory
       SANTOS, Christine
       KOFFAS, Mattheos
 
<120>  STRAINS FOR THE PRODUCTION OF FLAVONOIDS FROM GLUCOSE

<130>  M0656.70216WO00

<150>  US 61/332,593
<151>  2010-05-07

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  1
gctcggtacc atgctcgcca tgagccccc                                         29


<210>  2
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  2
acgaagcttt tagacgggag attgctgcaa gagg                                   34


<210>  3
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  3
taaaccatgg tccgcagcga gtacgcag                                          28


<210>  4
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  4
acgaagcttt tatcgcggct ccctgagctg t                                      31


<210>  5
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  5
taaaccatgg tgatggctgg tgcttcttct t                                      31


<210>  6
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  6
gctcggtacc ttagagagga acgctgtgca agacg                                  35


<210>  7
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  7
gctcggtacc atggctgcgg ctgctgcc                                          28


<210>  8
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  8
acgaagcttt tacacaataa taccgtggct caacacg                                37


<210>  9
<211>  50
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  9
acgaagctta atcctaggaa ctgaaatgag ctgttgacaa ttaatcatcc                  50


<210>  10
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  10
acgaagcttc ttggatcccg atccggaaat tatcgcggct ccctgagctg t                51


<210>  11
<211>  50
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  11
gagttcgaac gatgtacaaa ctgaaatgag ctgttgacaa ttaatcatcc                  50


<210>  12
<211>  61
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  12
gctagcttcg tacgtgctga gcatatcaat tgattacaca ataataccgt ggctcaacac       60

g                                                                       61


<210>  13
<211>  75
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  13
gagttcgaac tcgagatact agtgtagatc tttggcctcg ctggccatgc tagcttcgta       60

cgtgctgagc atatc                                                        75


<210>  14
<211>  32
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  14
cttggatccg ccgacatcat aacggttctg gc                                     32


<210>  15
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  15
cttggatccg agttcgaact cgagatacta gtgtagatct ttggc                       45


<210>  16
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  16
taaaccatgg cgcctcgcc                                                    19


<210>  17
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  17
aatgtcgact tatgccagca tcttcagcag aacatt                                 36


<210>  18
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  18
gcactaacat atgggtgact gcgttgcccc                                        30


<210>  19
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  19
aatcctaggt tacttcggca ggtcgcc                                           27


<210>  20
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  20
taacatatgg tgatggctgg tgc                                               23


<210>  21
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  21
aatcctaggt tagagaggaa cgctgtgcaa gacg                                   34


<210>  22
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  22
tataccatgg ctgcggctgc tg                                                22


<210>  23
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  23
taagcggccg cttacacaat aataccgtgg ctcaacacg                              39


<210>  24
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  24
catgacgtcc cgcttacaga caagctgtga ccg                                    33


<210>  25
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  25
gcttcgtacg tgctgagcat atcaatt                                           27


<210>  26
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  26
taacggccgg ccccgacatc ataacggttc tggca                                  35


<210>  27
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  27
taaggatccc aacagataaa acgaaaggcc cagtct                                 36


<210>  28
<211>  14
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  28
gtacgcgcat gcgc                                                         14


<210>  29
<211>  14
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide primer

<400>  29
gatcgcgcat gcgc                                                         14


<210>  30
<211>  675
<212>  DNA
<213>  artificial sequence

<220>
<223>  P. lobata chalcone isomerase

<400>  30
atggctgcgg ctgctgccgt ggcgaccatt agcgcggtgc aagtggagtt tctggaattt       60

ccagcggtag tgaccagccc ggcatcaggc cgtacctatt ttcttggtgg cgctggggag      120

cgtggcctga cgattgaggg caagtttatc aagttcaccg gcattggcgt gtatttggaa      180

gataaggcgg ttagctccct ggcggcgaaa tggaaaggca aaccgagcga agaactggtg      240

gagaccctgg acttctaccg ggatatcata agcggtccct tcgagaaact gatccgtggc      300

agcaaaattc tgccactgtc gggcgtcgaa tacagcaaga aagtgatgga aaactgcgtg      360

gcgcatatga aaagcgtcgg aacctatggc gatgcggaag ccgctgccat cgagaagttc      420

gcggaggcct tcaaaaacgt gaattttcaa cctggcgcga ccgtgtttta tcggcaaagc      480

ccagatggcg ttctgggcct gagtttcagc gaggatgtga ccattcccga taatgaagcg      540

gcggtgattg aaaacaaagc cgtctccgct gcggtgttag aaaccatgat tggcgaacat      600

gcagtaagcc ccgatctgaa acgtagcttg gcgagccggt tacccgccgt gttgagccac      660

ggtattattg tgtaa                                                       675


<210>  31
<211>  224
<212>  PRT
<213>  artificial sequence

<220>
<223>  P. lobata chalcone isomerase

<400>  31

Met Ala Ala Ala Ala Ala Val Ala Thr Ile Ser Ala Val Gln Val Glu 
1               5                   10                  15      


Phe Leu Glu Phe Pro Ala Val Val Thr Ser Pro Ala Ser Gly Arg Thr 
            20                  25                  30          


Tyr Phe Leu Gly Gly Ala Gly Glu Arg Gly Leu Thr Ile Glu Gly Lys 
        35                  40                  45              


Phe Ile Lys Phe Thr Gly Ile Gly Val Tyr Leu Glu Asp Lys Ala Val 
    50                  55                  60                  


Ser Ser Leu Ala Ala Lys Trp Lys Gly Lys Pro Ser Glu Glu Leu Val 
65                  70                  75                  80  


Glu Thr Leu Asp Phe Tyr Arg Asp Ile Ile Ser Gly Pro Phe Glu Lys 
                85                  90                  95      


Leu Ile Arg Gly Ser Lys Ile Leu Pro Leu Ser Gly Val Glu Tyr Ser 
            100                 105                 110         


Lys Lys Val Met Glu Asn Cys Val Ala His Met Lys Ser Val Gly Thr 
        115                 120                 125             


Tyr Gly Asp Ala Glu Ala Ala Ala Ile Glu Lys Phe Ala Glu Ala Phe 
    130                 135                 140                 


Lys Asn Val Asn Phe Gln Pro Gly Ala Thr Val Phe Tyr Arg Gln Ser 
145                 150                 155                 160 


Pro Asp Gly Val Leu Gly Leu Ser Phe Ser Glu Asp Val Thr Ile Pro 
                165                 170                 175     


Asp Asn Glu Ala Ala Val Ile Glu Asn Lys Ala Val Ser Ala Ala Val 
            180                 185                 190         


Leu Glu Thr Met Ile Gly Glu His Ala Val Ser Pro Asp Leu Lys Arg 
        195                 200                 205             


Ser Leu Ala Ser Arg Leu Pro Ala Val Leu Ser His Gly Ile Ile Val 
    210                 215                 220                 


<210>  32
<211>  2082
<212>  DNA
<213>  artificial sequence

<220>
<223>  R. glutinis tyrosine ammonia lyase

<400>  32
atggcgcctc gcccgacttc gcaaagccag gcccgcactt gcccgacgac gcaggttacc       60

caagttgata tcgttgagaa aatgttggcg gctcctactg atagcacgct ggagctggac      120

ggttatagcc tgaatctggg tgatgtcgtg agcgctgcgc gtaagggtcg tcctgtccgt      180

gtcaaagata gcgatgaaat ccgcagcaaa atcgacaaga gcgttgaatt cctgcgcagc      240

caactgagca tgtcggttta cggtgtgacg accggctttg gcggctccgc ggacacgcgc      300

acggaggacg caattagcct gcaaaaggcg ttgctggaac accagctgtg tggtgtgttg      360

ccgagcagct tcgacagctt tcgcttgggt cgtggtctgg agaatagcct gccgttggaa      420

gtcgttcgcg gtgcaatgac cattcgtgtg aattcgctga cccgtggcca tagcgctgtt      480

cgtctggttg ttctggaagc actgacgaac tttctgaacc acggtattac cccgattgtt      540

ccgctgcgcg gtacgatctc cgcgagcggc gatctgtctc cactgtcgta cattgcagcg      600

gcgattagcg gtcacccgga tagcaaagtt cacgtggtcc atgaaggcaa agagaagatc      660

ctgtacgcgc gcgaagcgat ggcgctgttt aacctggagc cggtggtttt gggtccgaag      720

gagggcctgg gtctggtgaa tggtacggca gtctccgcga gcatggcaac gctggcactg      780

cacgacgcgc atatgttgag cctgttgagc caatcgctga ccgcgatgac cgtggaggcg      840

atggtcggtc acgcgggcag cttccatcca ttcctgcacg atgttacgcg tccgcacccg      900

acgcaaatcg aggtcgcggg taacattcgc aaactgctgg agggctcgcg cttcgcggtc      960

caccacgagg aagaggttaa ggtcaaggat gatgaaggca ttttgcgtca ggatcgttat     1020

ccgttgcgca cgagcccgca atggttgggt ccgctggtgt ccgacctgat tcacgctcat     1080

gccgtcttga cgatcgaagc gggtcaaagc accaccgata acccactgat cgatgttgag     1140

aataagacca gccatcacgg tggcaacttt caagcggcag cggttgccaa cacgatggaa     1200

aagacccgtc tgggcttggc ccaaatcggt aaactgaatt tcacccagct gacggagatg     1260

ctgaacgcgg gcatgaatcg tggcttgccg agctgcctgg cggctgaaga cccatccctg     1320

agctatcatt gcaaaggtct ggacattgcg gcggctgcat atacgagcga actgggccac     1380

ctggctaacc cggtcaccac ccacgtccaa ccggctgaaa tggcaaacca ggcggtgaat     1440

agcttggcgt tgattagcgc acgtcgtacc acggaatcta acgacgttct gtccctgctg     1500

ctggcaacgc acctgtactg cgtgctgcag gcgatcgacc tgcgtgcgat tgagttcgag     1560

ttcaagaaac agtttggtcc tgccattgtt agcctgatcg accaacactt tggtagcgcg     1620

atgacgggta gcaatctgcg tgatgagctg gttgaaaagg tcaataagac tctggccaag     1680

cgtttggagc aaaccaatag ctacgatctg gttccgcgct ggcacgacgc ttttagcttc     1740

gctgcaggca ctgttgtcga ggttctgtcc agcacgagcc tgagcttggc ggccgtgaac     1800

gcatggaagg ttgcggcagc cgagagcgcg atctccttga cgcgccaggt ccgtgaaacg     1860

ttttggtccg ctgcaagcac ctccagcccg gcgttgtctt acttgagccc gcgcacgcag     1920

atcctgtacg catttgtgcg tgaggaactg ggtgtcaaag cccgccgtgg tgacgtcttc     1980

ttgggtaaac aagaagttac catcggcagc aacgttagca agatttacga agccatcaag     2040

agcggccgta tcaacaatgt tctgctgaag atgctggcat aa                        2082


<210>  33
<211>  693
<212>  PRT
<213>  artificial sequence

<220>
<223>  R. glutinis tyrosine ammonia lyase

<400>  33

Met Ala Pro Arg Pro Thr Ser Gln Ser Gln Ala Arg Thr Cys Pro Thr 
1               5                   10                  15      


Thr Gln Val Thr Gln Val Asp Ile Val Glu Lys Met Leu Ala Ala Pro 
            20                  25                  30          


Thr Asp Ser Thr Leu Glu Leu Asp Gly Tyr Ser Leu Asn Leu Gly Asp 
        35                  40                  45              


Val Val Ser Ala Ala Arg Lys Gly Arg Pro Val Arg Val Lys Asp Ser 
    50                  55                  60                  


Asp Glu Ile Arg Ser Lys Ile Asp Lys Ser Val Glu Phe Leu Arg Ser 
65                  70                  75                  80  


Gln Leu Ser Met Ser Val Tyr Gly Val Thr Thr Gly Phe Gly Gly Ser 
                85                  90                  95      


Ala Asp Thr Arg Thr Glu Asp Ala Ile Ser Leu Gln Lys Ala Leu Leu 
            100                 105                 110         


Glu His Gln Leu Cys Gly Val Leu Pro Ser Ser Phe Asp Ser Phe Arg 
        115                 120                 125             


Leu Gly Arg Gly Leu Glu Asn Ser Leu Pro Leu Glu Val Val Arg Gly 
    130                 135                 140                 


Ala Met Thr Ile Arg Val Asn Ser Leu Thr Arg Gly His Ser Ala Val 
145                 150                 155                 160 


Arg Leu Val Val Leu Glu Ala Leu Thr Asn Phe Leu Asn His Gly Ile 
                165                 170                 175     


Thr Pro Ile Val Pro Leu Arg Gly Thr Ile Ser Ala Ser Gly Asp Leu 
            180                 185                 190         


Ser Pro Leu Ser Tyr Ile Ala Ala Ala Ile Ser Gly His Pro Asp Ser 
        195                 200                 205             


Lys Val His Val Val His Glu Gly Lys Glu Lys Ile Leu Tyr Ala Arg 
    210                 215                 220                 


Glu Ala Met Ala Leu Phe Asn Leu Glu Pro Val Val Leu Gly Pro Lys 
225                 230                 235                 240 


Glu Gly Leu Gly Leu Val Asn Gly Thr Ala Val Ser Ala Ser Met Ala 
                245                 250                 255     


Thr Leu Ala Leu His Asp Ala His Met Leu Ser Leu Leu Ser Gln Ser 
            260                 265                 270         


Leu Thr Ala Met Thr Val Glu Ala Met Val Gly His Ala Gly Ser Phe 
        275                 280                 285             


His Pro Phe Leu His Asp Val Thr Arg Pro His Pro Thr Gln Ile Glu 
    290                 295                 300                 


Val Ala Gly Asn Ile Arg Lys Leu Leu Glu Gly Ser Arg Phe Ala Val 
305                 310                 315                 320 


His His Glu Glu Glu Val Lys Val Lys Asp Asp Glu Gly Ile Leu Arg 
                325                 330                 335     


Gln Asp Arg Tyr Pro Leu Arg Thr Ser Pro Gln Trp Leu Gly Pro Leu 
            340                 345                 350         


Val Ser Asp Leu Ile His Ala His Ala Val Leu Thr Ile Glu Ala Gly 
        355                 360                 365             


Gln Ser Thr Thr Asp Asn Pro Leu Ile Asp Val Glu Asn Lys Thr Ser 
    370                 375                 380                 


His His Gly Gly Asn Phe Gln Ala Ala Ala Val Ala Asn Thr Met Glu 
385                 390                 395                 400 


Lys Thr Arg Leu Gly Leu Ala Gln Ile Gly Lys Leu Asn Phe Thr Gln 
                405                 410                 415     


Leu Thr Glu Met Leu Asn Ala Gly Met Asn Arg Gly Leu Pro Ser Cys 
            420                 425                 430         


Leu Ala Ala Glu Asp Pro Ser Leu Ser Tyr His Cys Lys Gly Leu Asp 
        435                 440                 445             


Ile Ala Ala Ala Ala Tyr Thr Ser Glu Leu Gly His Leu Ala Asn Pro 
    450                 455                 460                 


Val Thr Thr His Val Gln Pro Ala Glu Met Ala Asn Gln Ala Val Asn 
465                 470                 475                 480 


Ser Leu Ala Leu Ile Ser Ala Arg Arg Thr Thr Glu Ser Asn Asp Val 
                485                 490                 495     


Leu Ser Leu Leu Leu Ala Thr His Leu Tyr Cys Val Leu Gln Ala Ile 
            500                 505                 510         


Asp Leu Arg Ala Ile Glu Phe Glu Phe Lys Lys Gln Phe Gly Pro Ala 
        515                 520                 525             


Ile Val Ser Leu Ile Asp Gln His Phe Gly Ser Ala Met Thr Gly Ser 
    530                 535                 540                 


Asn Leu Arg Asp Glu Leu Val Glu Lys Val Asn Lys Thr Leu Ala Lys 
545                 550                 555                 560 


Arg Leu Glu Gln Thr Asn Ser Tyr Asp Leu Val Pro Arg Trp His Asp 
                565                 570                 575     


Ala Phe Ser Phe Ala Ala Gly Thr Val Val Glu Val Leu Ser Ser Thr 
            580                 585                 590         


Ser Leu Ser Leu Ala Ala Val Asn Ala Trp Lys Val Ala Ala Ala Glu 
        595                 600                 605             


Ser Ala Ile Ser Leu Thr Arg Gln Val Arg Glu Thr Phe Trp Ser Ala 
    610                 615                 620                 


Ala Ser Thr Ser Ser Pro Ala Leu Ser Tyr Leu Ser Pro Arg Thr Gln 
625                 630                 635                 640 


Ile Leu Tyr Ala Phe Val Arg Glu Glu Leu Gly Val Lys Ala Arg Arg 
                645                 650                 655     


Gly Asp Val Phe Leu Gly Lys Gln Glu Val Thr Ile Gly Ser Asn Val 
            660                 665                 670         


Ser Lys Ile Tyr Glu Ala Ile Lys Ser Gly Arg Ile Asn Asn Val Leu 
        675                 680                 685             


Leu Lys Met Leu Ala 
    690             


<210>  34
<211>  1635
<212>  DNA
<213>  artificial sequence

<220>
<223>  P. crispus 4-coumarate:coA ligase

<400>  34
atgggtgact gcgttgcccc gaaagaggat ctgatcttcc gcagcaaact gccggacatt       60

tacattccaa agcatctgcc gctgcatacg tattgttttg agaatatcag caaggttggc      120

gacaagagct gtctgatcaa cggcgcaacc ggcgaaacgt ttacctacag ccaggtcgag      180

ctgctgtccc gtaaagttgc cagcggcctg aacaagctgg gcattcaaca aggtgatacc      240

attatgctgt tgctgccgaa ttccccggag tactttttcg ctttcctggg tgcgagctat      300

cgcggtgcaa tcagcaccat ggcgaatcca ttctttacca gcgcagaagt gatcaagcaa      360

ctgaaagcga gccaagcgaa gctgattatc acccaggcat gctatgttga caaggtcaag      420

gactacgcag cggagaaaaa catccagatc atttgtattg acgatgcacc gcaggattgc      480

ctgcacttta gcaagctgat ggaagcggat gagagcgaaa tgccggaagt ggtcattaac      540

agcgatgatg tggtggcatt gccgtacagc tctggcacca ccggcctgcc gaaaggcgtt      600

atgctgaccc acaagggtct ggttacgagc gttgcacaac aggtggatgg tgataacccg      660

aacctgtata tgcactccga ggatgtcatg atctgcatcc tgccactgtt ccatatctat      720

agcctgaacg ctgttctgtg ttgtggtctg cgtgcgggcg tcaccattct gatcatgcaa      780

aagttcgaca ttgtgccgtt tctggagctg attcagaagt ataaggttac gattggtccg      840

tttgtcccgc cgatcgtgct ggccatcgcg aaaagcccgg tcgttgacaa gtacgacttg      900

tctagcgtgc gcaccgtcat gagcggtgca gcgccgctgg gtaaagagtt ggaggacgct      960

gtccgtgcga aattcccgaa cgcgaagctg ggtcaaggct atggcatgac cgaagccggt     1020

ccggtcctgg cgatgtgtct ggcgttcgcc aaagagccgt atgagattaa gtctggcgca     1080

tgcggtaccg ttgtgcgtaa tgccgagatg aaaatcgttg acccagaaac gaatgcgtct     1140

ctgccgcgta atcagcgtgg tgagatttgc atccgtggtg atcagattat gaaaggttac     1200

ctgaatgacc cggaaagcac ccgcaccacg atcgacgaag agggttggtt gcacacgggt     1260

gacattggtt tcatcgacga tgacgatgaa ctgttcattg tcgatcgttt gaaagaaatc     1320

attaagtaca aaggttttca agttgctccg gcggagttgg aagcactgct gctgacgcac     1380

ccgacgatca gcgatgccgc ggtggttccg atgattgacg agaaagcggg tgaagtgcca     1440

gtggcgtttg tcgtgcgtac caatggtttt accacgaccg aagaagaaat caaacaattt     1500

gtgagcaaac aggtcgtgtt ctacaaacgt atcttccgcg tcttcttcgt tgacgctatt     1560

ccgaaatccc cgagcggcaa gattttgcgt aaggatctgc gcgctcgtat tgcgagcggc     1620

gacctgccga agtaa                                                      1635


<210>  35
<211>  544
<212>  PRT
<213>  artificial sequence

<220>
<223>  P. crispus 4-coumarate:coA ligase

<400>  35

Met Gly Asp Cys Val Ala Pro Lys Glu Asp Leu Ile Phe Arg Ser Lys 
1               5                   10                  15      


Leu Pro Asp Ile Tyr Ile Pro Lys His Leu Pro Leu His Thr Tyr Cys 
            20                  25                  30          


Phe Glu Asn Ile Ser Lys Val Gly Asp Lys Ser Cys Leu Ile Asn Gly 
        35                  40                  45              


Ala Thr Gly Glu Thr Phe Thr Tyr Ser Gln Val Glu Leu Leu Ser Arg 
    50                  55                  60                  


Lys Val Ala Ser Gly Leu Asn Lys Leu Gly Ile Gln Gln Gly Asp Thr 
65                  70                  75                  80  


Ile Met Leu Leu Leu Pro Asn Ser Pro Glu Tyr Phe Phe Ala Phe Leu 
                85                  90                  95      


Gly Ala Ser Tyr Arg Gly Ala Ile Ser Thr Met Ala Asn Pro Phe Phe 
            100                 105                 110         


Thr Ser Ala Glu Val Ile Lys Gln Leu Lys Ala Ser Gln Ala Lys Leu 
        115                 120                 125             


Ile Ile Thr Gln Ala Cys Tyr Val Asp Lys Val Lys Asp Tyr Ala Ala 
    130                 135                 140                 


Glu Lys Asn Ile Gln Ile Ile Cys Ile Asp Asp Ala Pro Gln Asp Cys 
145                 150                 155                 160 


Leu His Phe Ser Lys Leu Met Glu Ala Asp Glu Ser Glu Met Pro Glu 
                165                 170                 175     


Val Val Ile Asn Ser Asp Asp Val Val Ala Leu Pro Tyr Ser Ser Gly 
            180                 185                 190         


Thr Thr Gly Leu Pro Lys Gly Val Met Leu Thr His Lys Gly Leu Val 
        195                 200                 205             


Thr Ser Val Ala Gln Gln Val Asp Gly Asp Asn Pro Asn Leu Tyr Met 
    210                 215                 220                 


His Ser Glu Asp Val Met Ile Cys Ile Leu Pro Leu Phe His Ile Tyr 
225                 230                 235                 240 


Ser Leu Asn Ala Val Leu Cys Cys Gly Leu Arg Ala Gly Val Thr Ile 
                245                 250                 255     


Leu Ile Met Gln Lys Phe Asp Ile Val Pro Phe Leu Glu Leu Ile Gln 
            260                 265                 270         


Lys Tyr Lys Val Thr Ile Gly Pro Phe Val Pro Pro Ile Val Leu Ala 
        275                 280                 285             


Ile Ala Lys Ser Pro Val Val Asp Lys Tyr Asp Leu Ser Ser Val Arg 
    290                 295                 300                 


Thr Val Met Ser Gly Ala Ala Pro Leu Gly Lys Glu Leu Glu Asp Ala 
305                 310                 315                 320 


Val Arg Ala Lys Phe Pro Asn Ala Lys Leu Gly Gln Gly Tyr Gly Met 
                325                 330                 335     


Thr Glu Ala Gly Pro Val Leu Ala Met Cys Leu Ala Phe Ala Lys Glu 
            340                 345                 350         


Pro Tyr Glu Ile Lys Ser Gly Ala Cys Gly Thr Val Val Arg Asn Ala 
        355                 360                 365             


Glu Met Lys Ile Val Asp Pro Glu Thr Asn Ala Ser Leu Pro Arg Asn 
    370                 375                 380                 


Gln Arg Gly Glu Ile Cys Ile Arg Gly Asp Gln Ile Met Lys Gly Tyr 
385                 390                 395                 400 


Leu Asn Asp Pro Glu Ser Thr Arg Thr Thr Ile Asp Glu Glu Gly Trp 
                405                 410                 415     


Leu His Thr Gly Asp Ile Gly Phe Ile Asp Asp Asp Asp Glu Leu Phe 
            420                 425                 430         


Ile Val Asp Arg Leu Lys Glu Ile Ile Lys Tyr Lys Gly Phe Gln Val 
        435                 440                 445             


Ala Pro Ala Glu Leu Glu Ala Leu Leu Leu Thr His Pro Thr Ile Ser 
    450                 455                 460                 


Asp Ala Ala Val Val Pro Met Ile Asp Glu Lys Ala Gly Glu Val Pro 
465                 470                 475                 480 


Val Ala Phe Val Val Arg Thr Asn Gly Phe Thr Thr Thr Glu Glu Glu 
                485                 490                 495     


Ile Lys Gln Phe Val Ser Lys Gln Val Val Phe Tyr Lys Arg Ile Phe 
            500                 505                 510         


Arg Val Phe Phe Val Asp Ala Ile Pro Lys Ser Pro Ser Gly Lys Ile 
        515                 520                 525             


Leu Arg Lys Asp Leu Arg Ala Arg Ile Ala Ser Gly Asp Leu Pro Lys 
    530                 535                 540                 