
                                SEQUENCE LISTING

<110> Pioneer Hi-Bred International, Inc.
      E.I. DuPont de Nemours and Company
      
<120> Maize ACC Synthase 3 Gene and Protein
  and Uses Thereof

<130> 3705-PCT

<150> US 61/332,069       
<151> 2010-05-06  

<150> US 12/897,489      
<151> 2010-10-04

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 2765
<212> DNA
<213> Zea mays

<220> 
<221> 5'UTR          
<222> (214)...(372)

<220> 
<221> exon           
<222> (373)...(714)

<220> 
<221> intron         
<222> (715)...(818)

<220> 
<221> exon           
<222> (819)...(979)

<220> 
<221> intron         
<222> (980)...(1106)


<220> 
<221> exon           
<222> (1107)...(1992)


<220> 
<221> 3'UTR          
<222> (1993)...(2203)

<400> 1
tagtagacta aagctcccgg ctttcccttt attgttacta ttattatatc gtttgatttg 60
cagcggacag tctggtcgtt tccgtctgct ctggaccctg ccttcaattc tccgcctttt 120
gacatcttgg gtggtttcac acgggtatat atacttgact cgcctatata tgccaccccc 180
cacacacttg gccagatcga ccaccgcatc acacagcagc agcctcttgt gctcctctct 240
gtcctttgct tgctcatccc tcgacctcga gcggcggcgg cggtcactgc actgcgtgtg 300
cgtatccatc catcttgcag gcatcgtcgt ctgcaccgcg cgcttgtgcg tgagccaagg 360
gcggcagagg cgatgggtgg caagctgttg ctgggcgcga gccagagccg ccacgcgcac 420
gcggtggcgt cgcctcccct gtcaaaggtg gccacttccg gcctccacgg cgaggactcg 480
ccctacttcg ccgggtggaa agcctacgac gagaacccct acgacgccgt ctccaacccc 540
ggcggcgtca ttcagatggg cctcgccgag aaccaggtgt ccttcgacct cctcgagggg 600
tacctcaggg accacccgga ggccgcgggc tggggcggct ccggctccgg cgtcgccagc 660
ttcagggaca acgcgctgtt ccaggactac cacggcctca aggccttcag aaaggtgcgt 720
ggtgaccccg agcgcctgct ctgctcgtct tgttcggtgt cctggataga ccctgcctgg 780
acaagtgcaa cgtactgacg gcgtcatttt gcttgcaggc gatggccaac ttcatggaga 840
aggttagggg cggcaaggcc cggtttgacc ccgaccgcat cgtgctcacc gccggcgcca 900
cggcagctaa cgagctgctc acgttcgtcc tggccaaccc gggagacgcg ctgctgatcc 960
ctactcctta ctatcctggg taagcggagc gctaagaagc atccagcatg ctgcatgcat 1020
gcacggaccc ccggcgctat gctactgtta caccgcaaca gtgctctaca cagacagaca 1080
gacaaactga catgctctgc ttgcagtttc gacagagacc tgcggtggag gacgggggtg 1140
aacatcgtgc cggtgcactg cgacagcgcc aacgggttcc aggtcacggc cgccgcgctc 1200
caggcggcgt acgaggaggc cgaggcagcg gggacgcgcg tccgcgccgt cctgctcacc 1260
aacccgtcca acccgctggg caccaccgtg acgcggccgg ccctcgagga cgtgctcgac 1320
ttcgtggccc gcaacaacat ccacctcatc tccgacgaga tatactccgg ctcggtcttc 1380
gcggcgccgg acctggtcag cgtggcggag ctggtcgagt cccgcggcga ccccggcgtc 1440
gcggagcgcg tccacatcgt gtacagcctg tccaaggacc tgggcctccc ggggttccgc 1500
gtcggcgtcg tgtactcgta caacgacgcc gtggtcaccg cggcgcgccg catgtccagc 1560
ttcacgctcg tgtcgtcgca gacgcagaag acgctcgccg ccatgctctc ggacgccggg 1620
ttcgcggacg cctacgtccg caccaaccgc cagcgcctcc gggcgcggca cgaccacgtc 1680
gtcgccgggc tggcccgcgc gggcgtgccg tgcctccgcg gcaacgccgg gctgttcgtg 1740
tggatggaca tgaggcggct gctcggcgag gccaccaccg tcgccggcga gctccgcctg 1800
tgggaccgga tgctgcggga ggcgaagctc aacatctcgc cgggctcgtc gtgccattgc 1860
tcggagcctg gctggttcag ggtgtgcttc gccaacatga gcctggacac gctggatgtt 1920
gcactcgcta ggatgagccg cttcgtagac acgtggaaca aggaaacgac agcgtcgacg 1980
cagcagcact agcagcagca gcagcatacg aagtaaattt tttggagggt aaattacgtc 2040
attggacaga ttaaatcaca gagtagttat acagggggat tcttttatgg tttttcgatt 2100
gatggtaaca tcgattttgt aacaataact atcgcctctc agatggagga gggacacata 2160
tatgtatgta tttataaaaa ttcttacttt ggcccaagca aaagcgtctc cgctacacat 2220
tcagtattat tgttttgctt cgtttttccc tcctgagttg tggcaaacaa acacagatga 2280
cgtatgtttg ctcattcatt catatattta tatctgcctg gaagcgaacc taaattagca 2340
acaagagaga acatgtccat tccgagtact gagaataagt gcagcagaat gaatgctggt 2400
tgttggattt aaattagatg gatgttgttt gtcgtcagaa aatggagaga gagaaatcag 2460
ttagcgaatc cctttctcgt tttatactgc tggttttcta tacttttgaa gaaaaaaaac 2520
tgttttgctg aggtgttcga tgttgtaaat tactgattga cttcatgtga ttgcgtaaca 2580
tgttactgga cggaggttta cttctataca tgagtagtgt cataccaaac caaaaaaaaa 2640
agggaatcga aagtcttgat agcggacgtg ttgattaaag aaaaagaaac cgagttccaa 2700
atgtcaaccc ttccttaagc cggtagtaag ctttgtcaac cagctaaata agggaacgca 2760
tatcc                                                             2765

<210> 2
<211> 1759
<212> DNA
<213> Zea mays

<220> 
<221> CDS            
<222> (160)...(1548)

<400> 2
cagcagcagc ctcttgtgct cctctctgtc ctttgcttgc tcatccctcg acctcgagcg 60
gcggcggcgg tcactgcact gcgtgtgcgt atccatccat cttgcaggca tcgtcgtctg 120
caccgcgcgc ttgtgcgtga gccaagggcg gcagaggcg atg ggt ggc aag ctg    174
                                           Met Gly Gly Lys Leu
                                            1               5
 
ttg ctg ggc gcg agc cag agc cgc cac gcg cac gcg gtg gcg tcg cct   222
Leu Leu Gly Ala Ser Gln Ser Arg His Ala His Ala Val Ala Ser Pro
                 10                  15                  20
 
ccc ctg tca aag gtg gcc act tcc ggc ctc cac ggc gag gac tcg ccc   270
Pro Leu Ser Lys Val Ala Thr Ser Gly Leu His Gly Glu Asp Ser Pro
             25                  30                  35
 
tac ttc gcc ggg tgg aaa gcc tac gac gag aac ccc tac gac gcc gtc   318
Tyr Phe Ala Gly Trp Lys Ala Tyr Asp Glu Asn Pro Tyr Asp Ala Val
         40                  45                  50
 
tcc aac ccc ggc ggc gtc att cag atg ggc ctc gcc gag aac cag gtg   366
Ser Asn Pro Gly Gly Val Ile Gln Met Gly Leu Ala Glu Asn Gln Val
     55                  60                  65
 
tcc ttc gac ctc ctc gag ggg tac ctc agg gac cac ccg gag gcc gcg   414
Ser Phe Asp Leu Leu Glu Gly Tyr Leu Arg Asp His Pro Glu Ala Ala
 70                  75                  80                  85
 
ggc tgg ggc ggc tcc ggc tcc ggc gtc gcc agc ttc agg gac aac gcg   462
Gly Trp Gly Gly Ser Gly Ser Gly Val Ala Ser Phe Arg Asp Asn Ala
                 90                  95                 100
 
ctg ttc cag gac tac cac ggc ctc aag gcc ttc aga aag gcg atg gcc   510
Leu Phe Gln Asp Tyr His Gly Leu Lys Ala Phe Arg Lys Ala Met Ala
            105                 110                 115
 
aac ttc atg gag aag gtt agg ggc ggc aag gcc cgg ttt gac ccc gac   558
Asn Phe Met Glu Lys Val Arg Gly Gly Lys Ala Arg Phe Asp Pro Asp
        120                 125                 130
 
cgc atc gtg ctc acc gcc ggc gcc acg gca gct aac gag ctg ctc acg   606
Arg Ile Val Leu Thr Ala Gly Ala Thr Ala Ala Asn Glu Leu Leu Thr
    135                 140                 145
 
ttc gtc ctg gcc aac ccg gga gac gcg ctg ctg atc cct act cct tac   654
Phe Val Leu Ala Asn Pro Gly Asp Ala Leu Leu Ile Pro Thr Pro Tyr
150                 155                 160                 165
 
tat cct ggt ttc gac aga gac ctg cgg tgg agg acg ggg gtg aac atc   702
Tyr Pro Gly Phe Asp Arg Asp Leu Arg Trp Arg Thr Gly Val Asn Ile
                170                 175                 180
 
gtg ccg gtg cac tgc gac agc gcc aac ggg ttc cag gtc acg gcc gcc   750
Val Pro Val His Cys Asp Ser Ala Asn Gly Phe Gln Val Thr Ala Ala
            185                 190                 195
 
gcg ctc cag gcg gcg tac gag gag gcc gag gca gcg ggg acg cgc gtc   798
Ala Leu Gln Ala Ala Tyr Glu Glu Ala Glu Ala Ala Gly Thr Arg Val
        200                 205                 210
 
cgc gcc gtc ctg ctc acc aac ccg tcc aac ccg ctg ggc acc acc gtg   846
Arg Ala Val Leu Leu Thr Asn Pro Ser Asn Pro Leu Gly Thr Thr Val
    215                 220                 225
 
acg cgg ccg gcc ctc gag gac gtg ctc gac ttc gtg gcc cgc aac aac   894
Thr Arg Pro Ala Leu Glu Asp Val Leu Asp Phe Val Ala Arg Asn Asn
230                 235                 240                 245
 
atc cac ctc atc tcc gac gag ata tac tcc ggc tcg gtc ttc gcg gcg   942
Ile His Leu Ile Ser Asp Glu Ile Tyr Ser Gly Ser Val Phe Ala Ala
                250                 255                 260
 
ccg gac ctg gtc agc gtg gcg gag ctg gtc gag tcc cgc ggc gac ccc   990
Pro Asp Leu Val Ser Val Ala Glu Leu Val Glu Ser Arg Gly Asp Pro
            265                 270                 275
 
ggc gtc gcg gag cgc gtc cac atc gtg tac agc ctg tcc aag gac ctg   1038
Gly Val Ala Glu Arg Val His Ile Val Tyr Ser Leu Ser Lys Asp Leu
        280                 285                 290
 
ggc ctc ccg ggg ttc cgc gtc ggc gtc gtg tac tcg tac aac gac gcc   1086
Gly Leu Pro Gly Phe Arg Val Gly Val Val Tyr Ser Tyr Asn Asp Ala
    295                 300                 305
 
gtg gtc acc gcg gcg cgc cgc atg tcc agc ttc acg ctc gtg tcg tcg   1134
Val Val Thr Ala Ala Arg Arg Met Ser Ser Phe Thr Leu Val Ser Ser
310                 315                 320                 325
 
cag acg cag aag acg ctc gcc gcc atg ctc tcg gac gcc ggg ttc gcg   1182
Gln Thr Gln Lys Thr Leu Ala Ala Met Leu Ser Asp Ala Gly Phe Ala
                330                 335                 340
 
gac gcc tac gtc cgc acc aac cgc cag cgc ctc cgg gcg cgg cac gac   1230
Asp Ala Tyr Val Arg Thr Asn Arg Gln Arg Leu Arg Ala Arg His Asp
            345                 350                 355
 
cac gtc gtc gcc ggg ctg gcc cgc gcg ggc gtg ccg tgc ctc cgc ggc   1278
His Val Val Ala Gly Leu Ala Arg Ala Gly Val Pro Cys Leu Arg Gly
        360                 365                 370
 
aac gcc ggg ctg ttc gtg tgg atg gac atg agg cgg ctg ctc ggc gag   1326
Asn Ala Gly Leu Phe Val Trp Met Asp Met Arg Arg Leu Leu Gly Glu
    375                 380                 385
 
gcc acc acc gtc gcc ggc gag ctc cgc ctg tgg gac cgg atg ctg cgg   1374
Ala Thr Thr Val Ala Gly Glu Leu Arg Leu Trp Asp Arg Met Leu Arg
390                 395                 400                 405
 
gag gcg aag ctc aac atc tcg ccg ggc tcg tcg tgc cat tgc tcg gag   1422
Glu Ala Lys Leu Asn Ile Ser Pro Gly Ser Ser Cys His Cys Ser Glu
                410                 415                 420
 
cct ggc tgg ttc agg gtg tgc ttc gcc aac atg agc ctg gac acg ctg   1470
Pro Gly Trp Phe Arg Val Cys Phe Ala Asn Met Ser Leu Asp Thr Leu
            425                 430                 435
 
gat gtt gca ctc gct agg atg agc cgc ttc gta gac acg tgg aac aag   1518
Asp Val Ala Leu Ala Arg Met Ser Arg Phe Val Asp Thr Trp Asn Lys
        440                 445                 450
 
gaa acg aca gcg tcg acg cag cag cac tag cagcagcagc agcatacgaa     1568
Glu Thr Thr Ala Ser Thr Gln Gln His  *
    455                 460
 
gtaaattttt tggagggtaa attacgtcat tggacagatt aaatcacaga gtagttatac 1628
agggggattc ttttatggtt tttcgattga tggtaacatc gattttgtaa caataactat 1688
cgcctctcag atggaggagg gacacatata tgtatgtatt tataaaaatt cttactttgg 1748
cccaagcaaa a                                                      1759

<210> 3
<211> 462
<212> PRT
<213> Zea mays

<400> 3
Met Gly Gly Lys Leu Leu Leu Gly Ala Ser Gln Ser Arg His Ala His
 1               5                  10                  15      
Ala Val Ala Ser Pro Pro Leu Ser Lys Val Ala Thr Ser Gly Leu His
            20                  25                  30          
Gly Glu Asp Ser Pro Tyr Phe Ala Gly Trp Lys Ala Tyr Asp Glu Asn
        35                  40                  45              
Pro Tyr Asp Ala Val Ser Asn Pro Gly Gly Val Ile Gln Met Gly Leu
    50                  55                  60                  
Ala Glu Asn Gln Val Ser Phe Asp Leu Leu Glu Gly Tyr Leu Arg Asp
65                  70                  75                  80  
His Pro Glu Ala Ala Gly Trp Gly Gly Ser Gly Ser Gly Val Ala Ser
                85                  90                  95      
Phe Arg Asp Asn Ala Leu Phe Gln Asp Tyr His Gly Leu Lys Ala Phe
            100                 105                 110         
Arg Lys Ala Met Ala Asn Phe Met Glu Lys Val Arg Gly Gly Lys Ala
        115                 120                 125             
Arg Phe Asp Pro Asp Arg Ile Val Leu Thr Ala Gly Ala Thr Ala Ala
    130                 135                 140                 
Asn Glu Leu Leu Thr Phe Val Leu Ala Asn Pro Gly Asp Ala Leu Leu
145                 150                 155                 160 
Ile Pro Thr Pro Tyr Tyr Pro Gly Phe Asp Arg Asp Leu Arg Trp Arg
                165                 170                 175     
Thr Gly Val Asn Ile Val Pro Val His Cys Asp Ser Ala Asn Gly Phe
            180                 185                 190         
Gln Val Thr Ala Ala Ala Leu Gln Ala Ala Tyr Glu Glu Ala Glu Ala
        195                 200                 205             
Ala Gly Thr Arg Val Arg Ala Val Leu Leu Thr Asn Pro Ser Asn Pro
    210                 215                 220                 
Leu Gly Thr Thr Val Thr Arg Pro Ala Leu Glu Asp Val Leu Asp Phe
225                 230                 235                 240 
Val Ala Arg Asn Asn Ile His Leu Ile Ser Asp Glu Ile Tyr Ser Gly
                245                 250                 255     
Ser Val Phe Ala Ala Pro Asp Leu Val Ser Val Ala Glu Leu Val Glu
            260                 265                 270         
Ser Arg Gly Asp Pro Gly Val Ala Glu Arg Val His Ile Val Tyr Ser
        275                 280                 285             
Leu Ser Lys Asp Leu Gly Leu Pro Gly Phe Arg Val Gly Val Val Tyr
    290                 295                 300                 
Ser Tyr Asn Asp Ala Val Val Thr Ala Ala Arg Arg Met Ser Ser Phe
305                 310                 315                 320 
Thr Leu Val Ser Ser Gln Thr Gln Lys Thr Leu Ala Ala Met Leu Ser
                325                 330                 335     
Asp Ala Gly Phe Ala Asp Ala Tyr Val Arg Thr Asn Arg Gln Arg Leu
            340                 345                 350         
Arg Ala Arg His Asp His Val Val Ala Gly Leu Ala Arg Ala Gly Val
        355                 360                 365             
Pro Cys Leu Arg Gly Asn Ala Gly Leu Phe Val Trp Met Asp Met Arg
    370                 375                 380                 
Arg Leu Leu Gly Glu Ala Thr Thr Val Ala Gly Glu Leu Arg Leu Trp
385                 390                 395                 400 
Asp Arg Met Leu Arg Glu Ala Lys Leu Asn Ile Ser Pro Gly Ser Ser
                405                 410                 415     
Cys His Cys Ser Glu Pro Gly Trp Phe Arg Val Cys Phe Ala Asn Met
            420                 425                 430         
Ser Leu Asp Thr Leu Asp Val Ala Leu Ala Arg Met Ser Arg Phe Val
        435                 440                 445             
Asp Thr Trp Asn Lys Glu Thr Thr Ala Ser Thr Gln Gln His
    450                 455                 460         


<210> 4
<211> 482
<212> PRT
<213> Zea mays

<400> 4
Met Ile Ala Asp Glu Lys Pro Gln Pro Gln Leu Leu Ser Lys Lys Ala
 1               5                  10                  15      
Ala Cys Asn Ser His Gly Gln Asp Ser Ser Tyr Phe Leu Gly Trp Glu
            20                  25                  30          
Glu Tyr Glu Lys Asn Pro Tyr Asp Pro Val Ala Asn Pro Gly Gly Ile
        35                  40                  45              
Ile Gln Met Gly Leu Ala Glu Asn Gln Leu Ser Phe Asp Leu Leu Glu
    50                  55                  60                  
Ala Trp Leu Glu Ala Asn Pro Asp Ala Leu Gly Leu Arg Arg Gly Gly
65                  70                  75                  80  
Ala Ser Val Phe Arg Glu Leu Ala Leu Phe Gln Asp Tyr His Gly Met
                85                  90                  95      
Pro Ala Phe Lys Asn Ala Leu Ala Arg Phe Met Ser Glu Gln Arg Gly
            100                 105                 110         
Tyr Arg Val Thr Phe Asp Pro Ser Asn Ile Val Leu Thr Ala Gly Ala
        115                 120                 125             
Thr Ser Ala Asn Glu Ala Leu Met Phe Cys Leu Ala Asp His Gly Asp
    130                 135                 140                 
Ala Phe Leu Ile Pro Thr Pro Tyr Tyr Pro Gly Phe Asp Arg Asp Leu
145                 150                 155                 160 
Lys Trp Arg Thr Gly Ala Glu Ile Val Pro Val His Cys Thr Ser Gly
                165                 170                 175     
Asn Gly Phe Arg Leu Thr Arg Ala Ala Leu Asp Asp Ala Tyr Arg Arg
            180                 185                 190         
Ala Gln Lys Leu Arg Leu Arg Val Lys Gly Val Leu Ile Thr Asn Pro
        195                 200                 205             
Ser Asn Pro Leu Gly Thr Thr Ser Pro Arg Ala Asp Leu Glu Met Leu
    210                 215                 220                 
Val Asp Phe Val Ala Ala Lys Gly Ile His Leu Val Ser Asp Glu Ile
225                 230                 235                 240 
Tyr Ser Gly Thr Val Phe Ala Asp Pro Gly Phe Val Ser Val Leu Glu
                245                 250                 255     
Val Val Ala Ala Arg Ala Ala Thr Asp Asp Gly Val Val Gly Val Gly
            260                 265                 270         
Pro Leu Ser Asp Arg Val His Val Val Tyr Ser Leu Ser Lys Asp Leu
        275                 280                 285             
Gly Leu Pro Gly Phe Arg Val Gly Ala Ile Tyr Ser Ser Asn Ala Gly
    290                 295                 300                 
Val Val Ser Ala Ala Thr Lys Met Ser Ser Phe Gly Leu Val Ser Ser
305                 310                 315                 320 
Gln Thr Gln His Leu Leu Ala Ser Leu Leu Gly Asp Arg Asp Phe Thr
                325                 330                 335     
Arg Arg Tyr Ile Ala Glu Asn Thr Arg Arg Ile Arg Glu Arg Arg Glu
            340                 345                 350         
Gln Leu Ala Glu Gly Leu Ala Ala Val Gly Ile Glu Cys Leu Glu Ser
        355                 360                 365             
Asn Ala Gly Leu Phe Cys Trp Val Asn Met Arg Arg Leu Met Arg Ser
    370                 375                 380                 
Arg Ser Phe Glu Gly Glu Met Glu Leu Trp Lys Lys Val Val Phe Glu
385                 390                 395                 400 
Val Gly Leu Asn Ile Ser Pro Gly Ser Ser Cys His Cys Arg Glu Pro
                405                 410                 415     
Gly Trp Phe Arg Val Cys Phe Ala Asn Met Ser Ala Lys Thr Leu Asp
            420                 425                 430         
Val Ala Leu Gln Arg Leu Gly Ala Phe Ala Glu Ala Ala Thr Ala Gly
        435                 440                 445             
Arg Arg Val Leu Ala Pro Ala Arg Ser Ile Ser Leu Pro Val Arg Phe
    450                 455                 460                 
Ser Trp Ala Asn Arg Leu Thr Pro Gly Ser Ala Ala Asp Arg Lys Ala
465                 470                 475                 480 
Glu Arg
        


<210> 5
<211> 543
<212> DNA
<213> Artificial Sequence

<220> 
<223> segment of hp construct

<400> 5
cccggtacgc gccgccacgg acgacggcgt cgtcggcgtt gggccgctgt cggaccgcgt 60
gcacgtggtg tacagcctgt ccaaggacct gggcctcccg gggttccgcg tgggcgccat 120
ctactcgtcc aacgccggcg tggtctccgc ggccaccaag atgtcgagct tcggcctggt 180
gtcgtcccag acgcagcacc tcctggcgtc gctcctgggc gacagggact tcacgcggag 240
gtacatcgcg gagaacacgc ggcggatcag ggagcggcgc gagcagctgg cggagggcct 300
ggcggccgtg ggcatcgagt gcctggagag caacgcgggg ctcttctgct gggtcaacat 360
gcggcgcctg atgcggagcc ggtcgttcga gggcgagatg gagctgtgga agagggtggt 420
cttcgaggtg gggctcaaca tctccccggg ctcctcctgc cactgccggg agcccggctg 480
gttccgcgtc tgctaaaggg cgaattccag cacactggcg gccgttacta gtggatccga 540
gct                                                               543

<210> 6
<211> 1446
<212> DNA
<213> Zea mays

<400> 6
atgatcgccg acgagaagcc gcagccgcag ctgctgtcca agaaggccgc ctgcaacagc 60
cacggccagg actcgtccta cttcctgggg tgggaggagt atgagaaaaa cccatacgac 120
cccgtcgcca accccggcgg catcatccag atgggcctcg ccgagaacca gctgtccttc 180
gacctgctgg aggcgtggct ggaggccaac ccggacgcgc tcggcctccg ccggggaggc 240
gcctctgtat tccgcgagct cgcgctcttc caggactacc acggcatgcc ggccttcaag 300
aatgcattgg cgaggttcat gtcggagcaa cgtgggtacc gggtgacctt cgaccccagc 360
aacatcgtgc tcaccgccgg agccacctcg gccaacgagg ccctcatgtt ctgcctcgcc 420
gaccacggag acgcctttct catccccacg ccatactacc cagggttcga ccgtgacctc 480
aagtggcgca ccggcgcgga gatcgtcccc gtgcactgca cgagcggcaa cggcttccgg 540
ctgacgcgcg ccgcgctgga cgacgcgtac cggcgcgcgc agaagctgcg gctgcgcgtc 600
aagggcgtgc tcatcaccaa cccttccaac ccgctgggca ccacgtcgcc gcgcgccgac 660
ctggagatgc tggtggactt cgtggccgcc aagggcatcc acctggtgag cgacgagata 720
tactcgggca cggtcttcgc ggacccgggc ttcgtgagcg tcctcgaggt ggtggccgcg 780
cgcgccgcca cggacgacgg cgtcgtcggc gttgggccgc tgtcggaccg cgtgcacgtg 840
gtgtacagcc tgtccaagga cctgggcctc ccggggttcc gcgtgggcgc catctactcg 900
tccaacgccg gcgtggtctc cgcggccacc aagatgtcga gcttcggcct ggtgtcgtcc 960
cagacgcagc acctcctggc gtcgctcctg ggcgacaggg acttcacgcg gaggtacatc 1020
gcggagaaca cgcggcggat cagggagcgg cgcgagcagc tggcggaggg cctggcggcc 1080
gtgggcatcg agtgcctgga gagcaacgcg gggctcttct gctgggtcaa catgcggcgc 1140
ctgatgcgga gccggtcgtt cgagggcgag atggagctgt ggaagaaggt ggtcttcgag 1200
gtggggctca acatctcccc gggctcctcc tgccactgcc gggagcccgg ctggttccgc 1260
gtctgcttcg ccaacatgtc cgccaagacg ctcgacgtcg cgctccagcg cctgggcgcc 1320
ttcgcggagg ccgccaccgc ggggcgccgc gtgcttgccc ccgccaggag catcagcctc 1380
ccggtccgct tcagctgggc taaccgcctc accccgggct ccgccgccga ccggaaggcc 1440
gagcgg                                                            1446

<210> 7
<211> 8350
<212> DNA
<213> Artificial Sequence

<220> 
<223> ACS down-regulation expression cassette

<400> 7
gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 60
aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg 120
acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagcaag 180
ctggtacgat tgtaatacga ctcactatag ggcgaattga gcgctgttta aacgctcttc 240
aactggaaga gcggttacta ccggctggat ggcggggcct tgatcgtgca ccgccggcgt 300
ccggataagt gactagggtc acgtgaccct agtcacttat cgagctagtt accctatgag 360
gtgacatgaa gcgctcacgg ttactatgac ggttagcttc acgactgttg gtggcagtag 420
cgtacgactt agctatagtt ccggtagatc tgaagttcct attccgaagt tcctattctt 480
caaaaggtat aggaacttcc tcgaattgtt gtggtggggt atagaggttt gatataggtg 540
gaactgctgt agagcgtgga gatatagggg gaaagagaac gctgatgtga caagtgagtg 600
agatataggg ggagaaattt agggggaacg ccgaacacag tctaaagaag cttgggaccc 660
aaagcactct gttcgggggt tttttttttt gtctttcaac tttttgctgt aatgttattc 720
aaaataagaa aagcacttgg catggctaag aaatagagtt caacaactga acagtacagt 780
gtattatcaa tggcataaaa aacaaccctt acagcattgc cgtattttat tgatcaaaca 840
ttcaactcaa cactgacgag tggtcttcca ccgatcaacg gactaatgct gctttgtcag 900
atcaccggtt aagtgactag ggtcacgtga ccctagtcac ttaggttacc agagctggtc 960
acctttgtcc accaagatgg aactgcggcc gctcattaat taagtcaggc gcgcctctag 1020
ttgaagacac gttcatgtct tcatcgtaag aagacactca gtagtcttcg gccagaatgg 1080
ccatctggat tcagcaggcc tagaaggcca tttaaatcct gaggatctgg tcttcctaag 1140
gacccgggat atcacaagtt tgtacaaaaa agcaggctcc ggccagagtt acccggaccg 1200
aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc 1260
attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt 1320
gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata 1380
gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta 1440
aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt 1500
gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta 1560
catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt 1620
ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta 1680
ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta 1740
agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt 1800
aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc 1860
aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg 1920
ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac 1980
gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat 2040
tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc 2100
tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct 2160
cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc 2220
ccctctctac cttctctaga tcggcgttcc ggtccatgca tggttagggc ccggtagttc 2280
tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc 2340
gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc 2400
tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt 2460
tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca 2520
cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg 2580
gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt 2640
aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga 2700
tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca 2760
gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg 2820
ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact 2880
gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct 2940
aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag 3000
catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta 3060
taattatttt gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt 3120
ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc 3180
accctgttgt ttggtgttac ttctgcaggt cgactttaac ttagcctagg atccactagt 3240
aacggccgcc agtgtgctgg aattcgccct ttagcagacg cggaaccagc cgggctcccg 3300
gcagtggcag gaggagcccg gggagatgtt gagccccacc tcgaagacca ccctcttcca 3360
cagctccatc tcgccctcga acgaccggct ccgcatcagg cgccgcatgt tgacccagca 3420
gaagagcccc gcgttgctct ccaggcactc gatgcccacg gccgccaggc cctccgccag 3480
ctgctcgcgc cgctccctga tccgccgcgt gttctccgcg atgtacctcc gcgtgaagtc 3540
cctgtcgccc aggagcgacg ccaggaggtg ctgcgtctgg gacgacacca ggccgaagct 3600
cgacatcttg gtggccgcgg agaccacgcc ggcgttggac gagtagatgg cgcccacgcg 3660
gaaccccggg aggcccaggt ccttggacag gctgtacacc acgtgcacgc ggtccgacag 3720
cggcccaacg ccgacgacgc cgtcgtccgt ggcggcgcgc gcggccacca cctgcagtcg 3780
acgtgcaaag gtccgccttg tttctcctct gtctcttgat ctgactaatc ttggtttatg 3840
attcgttgag taattttggg gaaagcttcg tccacagttt tttttcgatg aacagtgccg 3900
cagtggcgct gatcttgtat gctatcctgc aatcgtggtg aacttatttc ttttatatcc 3960
tttactccca tgaaaaggct agtaatcttt ctcgatgtaa catcgtccag cactgctatt 4020
accgtgtggt ccatccgaca gtctggctga acacatcata cgatctatgg agcaaaaatc 4080
tatcttccct gttctttaat gaaggacgtc attttcatta gtatgatcta ggaatgttgc 4140
aacttgcaag gaggcgtttc tttctttgaa tttaactaac tcgttgagtg gccctgtttc 4200
tcggacgtaa ggcctttgct gctccacaca tgtccattcg aattttaccg tgtttagcaa 4260
gggcgaaaag tttgcatctt gatgatttag cttgactatg cgattgcttt cctggacccg 4320
tgcagctgga tcccggtacg cgccgccacg gacgacggcg tcgtcggcgt tgggccgctg 4380
tcggaccgcg tgcacgtggt gtacagcctg tccaaggacc tgggcctccc ggggttccgc 4440
gtgggcgcca tctactcgtc caacgccggc gtggtctccg cggccaccaa gatgtcgagc 4500
ttcggcctgg tgtcgtccca gacgcagcac ctcctggcgt cgctcctggg cgacagggac 4560
ttcacgcgga ggtacatcgc ggagaacacg cggcggatca gggagcggcg cgagcagctg 4620
gcggagggcc tggcggccgt gggcatcgag tgcctggaga gcaacgcggg gctcttctgc 4680
tgggtcaaca tgcggcgcct gatgcggagc cggtcgttcg agggcgagat ggagctgtgg 4740
aagagggtgg tcttcgaggt ggggctcaac atctccccgg gctcctcctg ccactgccgg 4800
gagcccggct ggttccgcgt ctgctaaagg gcgaattcca gcacactggc ggccgttact 4860
agtggatccg agctcgaatt ccggtccggg tcacccggtc cgggcctaga aggccgatct 4920
cccgggcacc cagctttctt gtacaaagtg gtgatatcgg accgattaaa ctttaattcg 4980
gtccgatgca tgtatacgaa gttcctattc cgaagttcct attctacata gagtatagga 5040
acttcacctg gtggcgccgc tagtggatcc cccgggctgc agtgcagcgt gacccggtcg 5100
tgcccctctc tagagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt 5160
ttttttgtca cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt 5220
actctacgaa taatataatc tatagtacta caataatatc agtgttttag agaatcatat 5280
aaatgaacag ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca 5340
gttttatctt tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat 5400
aatacttcat ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag 5460
actaattttt ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa 5520
actctatttt agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac 5580
taaaaattaa acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt 5640
tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc 5700
gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc 5760
tctggacccc tctcgagagt tccgctccac cgttggactt gctccgctgt cggcatccag 5820
aaattgcgtg gcggagcggc agacgtgagc cggcacggca ggcggcctcc tcctcctctc 5880
acggcaccgg cagctacggg ggattccttt cccaccgctc cttcgctttc ccttcctcgc 5940
ccgccgtaat aaatagacac cccctccaca ccctctttcc ccaacctcgt gttgttcgga 6000
gcgcacacac acacaaccag atctccccca aatccacccg tcggcacctc cgcttcaagg 6060
tacgccgctc gtcctccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca 6120
tgcatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt 6180
gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg 6240
attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca 6300
gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc 6360
ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg 6420
tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattctgt 6480
ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca 6540
tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat 6600
gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg 6660
tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct 6720
ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt 6780
taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat 6840
gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct 6900
attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca 6960
tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg 7020
gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtcgactt 7080
taacttagcc taggatccac acgacaccat gtcccccgag cgccgccccg tcgagatccg 7140
cccggccacc gccgccgaca tggccgccgt gtgcgacatc gtgaaccact acatcgagac 7200
ctccaccgtg aacttccgca ccgagccgca gaccccgcag gagtggatcg acgacctgga 7260
gcgcctccag gaccgctacc cgtggctcgt ggccgaggtg gagggcgtgg tggccggcat 7320
cgcctacgcc ggcccgtgga aggcccgcaa cgcctacgac tggaccgtgg agtccaccgt 7380
gtacgtgtcc caccgccacc agcgcctcgg cctcggctcc accctctaca cccacctcct 7440
caagagcatg gaggcccagg gcttcaagtc cgtggtggcc gtgatcggcc tcccgaacga 7500
cccgtccgtg cgcctccacg aggccctcgg ctacaccgcc cgcggcaccc tccgcgccgc 7560
cggctacaag cacggcggct ggcacgacgt cggcttctgg cagcgcgact tcgagctgcc 7620
ggccccgccg cgcccggtgc gcccggtgac gcagatctga gtcgaaacct agacttgtcc 7680
atcttctgga ttggccaact taattaatgt atgaaataaa aggatgcaca catagtgaca 7740
tgctaatcac tataatgtgg gcatcaaagt tgtgtgttat gtgtaattac tagttatctg 7800
aataaaagag aaagagatca tccatatttc ttatcctaaa tgaatgtcac gtgtctttat 7860
aattctttga tgaaccagat gcatttcatt aaccaaatcc atatacatat aaatattaat 7920
catatataat taatatcaat tgggttagca aaacaaatct agtctaggtg tgttttgcga 7980
attgcggccg ctctagcgta tacgaagttc ctattccgaa gttcctattc tctagaaagt 8040
ataggaactt ctgattccga tgacttcgta ggttcctagc tcaagccgct cgtgtccaag 8100
cgtcacttac gattagctaa tgattacggc atctaggacc gactagtaag tgactagggt 8160
cacgtgaccc tagtcactta tacgtagaat taattcattc cgattaatcg tggcctcttg 8220
ctcttcagga tgaagagcta tgtttaaacg tgcaagcgct actagacaat tcagtacatt 8280
aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat 8340
atcctgccac                                                        8350



<210> 8
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 8
acgagctgct cacgttcgt                                              19

<210> 9
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 9
ccaggatagt aaggagtagg gatcag                                      26

<210> 10
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> MGB probe

<400> 10
aacccgggag acgc                                                   14
