                               SEQUENCE LISTING	

<110> SANGAMO BIOSCIENCES, INC. et al.
 
<120> GENOME EDITING OF A ROSA LOCUS USING ZINC-FINGER NUCLEASES

<130> 8325-0078.41

<140> PCT/US2011/000726
<141> 2011-04-25

<150> 61/343,287
<151> 2010-04-26

<160> 92    

<170> PatentIn version 3.5

<210> 1
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 1
Thr Gly Glu Lys Pro 
1               5   


<210> 2
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 2
Thr Gly Gly Gln Arg Pro 
1               5       


<210> 3
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 3
Thr Gly Gln Lys Pro 
1               5   


<210> 4
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 4
Thr Gly Ser Gln Lys Pro 
1               5       


<210> 5
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 5
aaaaggtacc gggagtggat gaaggagttg                                        30


<210> 6
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 6
aaaaccgcgg cggatcacaa gcaataat                                          28


<210> 7
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 7
cttcgcggcc gcgatctgca actggagtct ttc                                    33


<210> 8
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 8
cttcgtttaa acgatctgca actggagtct ttc                                    33


<210> 9
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
gatcgcggcc gcgaagaagg gggaagggaa tc                                     32


<210> 10
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
gatcgtttaa acgaagaagg gggaagggaa tc                                     32


<210> 11
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
aaaaggtacc gcgtgtgaaa acacaaatgg                                        30


<210> 12
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
aaaaccgcgg aaggaaagag gcattcatgg                                        30


<210> 13
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
aaaaggtacc attatggagg ggaggactgg                                        30


<210> 14
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
aaaaccgcgg acatgtggca aacaggaga                                         29


<210> 15
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
tgtcttctga ggaccgccc                                                    19


<210> 16
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
ctgcccagaa gactcccgc                                                    19


<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Gln Ser Gly Asp Leu Thr Arg 
1               5           


<210> 18
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Thr Ser Gly Ser Leu Thr Arg 
1               5           


<210> 19
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Gln Ser Gly His Leu Ala Arg 
1               5           


<210> 20
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Gln Ser Ser Asp Leu Thr Arg 
1               5           


<210> 21
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Arg Ser Asp Asn Leu Ser Glu 
1               5           


<210> 22
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Gln Asn Ala His Arg Lys Thr 
1               5           


<210> 23
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 23
Asp Arg Ser Ala Arg Thr Arg 
1               5           


<210> 24
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Gln Ser Gly His Leu Ser Arg 
1               5           


<210> 25
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Arg Ser Asp Asp Leu Ser Lys 
1               5           


<210> 26
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Arg Asn Asp His Arg Lys Asn 
1               5           


<210> 27
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Asp Thr Thr Ser Leu Asp Arg 
1               5           


<210> 28
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 28
ctagaaagac tggagttgca gatcacga                                          28


<210> 29
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 29
gatgggcggg agtcttctgg gcaggctt                                          28


<210> 30
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 30
ctagaaagac tggagttgca gatcacga                                          28


<210> 31
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 31
ggctcgagtg agtcatcaga cttctaagat cagg                                   34


<210> 32
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 32
ggctcgagtt ttgataaggc tgcagaag                                          28


<210> 33
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 33
ctgaattcga atgggcggga gtcttctggg ca                                     32


<210> 34
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 34
ccaagcttgg aggtaggtgg ggtgagg                                           27


<210> 35
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 35
ccaagcttag tcgctctgag ttgttatc                                          28


<210> 36
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 36
ccaagctttc tgggagttct ctgctgcc                                          28


<210> 37
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 37
cattcgaatt cagaaagact ggagttgcag atc                                    33


<210> 38
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 38
Asp Arg Ser Asp Leu Ser Arg 
1               5           


<210> 39
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 39
Arg Ser Asp Asp Leu Thr Arg 
1               5           


<210> 40
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 40
Thr Ser Gly His Leu Ser Arg 
1               5           


<210> 41
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 41
Arg Ser Asp Asn Leu Ser Val 
1               5           


<210> 42
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 42
Arg Ser Ala Asn Leu Thr Arg 
1               5           


<210> 43
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 43
Gln Ser Asp His Leu Thr Lys 
1               5           


<210> 44
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 44
Asn Ser Ser Asn Leu Ser Arg 
1               5           


<210> 45
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 45
Arg Ser Asp His Leu Thr Lys 
1               5           


<210> 46
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 46
Asn Ser Asp His Leu Ser Arg 
1               5           


<210> 47
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 47
Arg Ser Asp His Leu Ser Arg 
1               5           


<210> 48
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 48
Arg Ser Asp His Leu Ser Glu 
1               5           


<210> 49
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 49
Arg Ser Ala Ala Leu Ala Arg 
1               5           


<210> 50
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 50
Arg Ser Asp His Leu Ser Thr 
1               5           


<210> 51
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 51
Gln Asn Ala His Arg Ile Thr 
1               5           


<210> 52
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 52
Arg Ser Ala Val Leu Ser Glu 
1               5           


<210> 53
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 53
Arg Ser Ala Asn Leu Ala Arg 
1               5           


<210> 54
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 54
Arg Ser Asp Asn Leu Arg Glu 
1               5           


<210> 55
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 55
Asp Asn Arg Asp Arg Ile Lys 
1               5           


<210> 56
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 56
Arg Ser Asp Thr Leu Ser Glu 
1               5           


<210> 57
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 57
Gln Ser Ser His Leu Ala Arg 
1               5           


<210> 58
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 58
Arg Ser Asp Asn Leu Thr Arg 
1               5           


<210> 59
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 59
Thr Ser Ser Thr Arg Lys Thr 
1               5           


<210> 60
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 60
Thr Ser Gly Asn Leu Thr Arg 
1               5           


<210> 61
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 61
Gln Ser Gly Asn Leu Ala Arg 
1               5           


<210> 62
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 62
Arg Ser Asp Ala Leu Ser Val 
1               5           


<210> 63
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 63
Asp Ser Ser His Arg Thr Arg 
1               5           


<210> 64
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 64
Arg Ser Asp Val Leu Ser Glu 
1               5           


<210> 65
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 65
Gln Arg Ser Asn Leu Val Arg 
1               5           


<210> 66
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 66
Arg Ser Asp His Leu Thr Gln 
1               5           


<210> 67
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 67
Gln Ser Gly His Leu Gln Arg 
1               5           


<210> 68
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 68
Asp Arg Ser His Leu Ala Arg 
1               5           


<210> 69
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 69
Gln Arg Asn His Arg Thr Thr 
1               5           


<210> 70
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 70
Thr Lys Arg Ser Leu Ile Glu 
1               5           


<210> 71
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 71
Thr Ser Ser Asn Leu Ser Arg 
1               5           


<210> 72
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 72
Arg Ser Asp His Leu Ser Ala 
1               5           


<210> 73
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 73
Gln Asn Asp Asn Arg Ile Lys 
1               5           


<210> 74
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 74
Asn Asn Arg Asp Leu Ile Asn 
1               5           


<210> 75
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 75
ctgagaaggg tgcggccttt tctccgcc                                          28


<210> 76
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 76
acgggggagg ggagtgttgc aatacctt                                          28


<210> 77
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 77
tcctgagaag ggtgcggcct tttctccg                                          28


<210> 78
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 78
ggggagggga gtgttgcaat acctttct                                          28


<210> 79
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 79
gacaggaggc agcagagaac tcccagaa                                          28


<210> 80
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 80
tctgaggacc gccctgggcc tggaagat                                          28


<210> 81
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 81
gaagacagga ggcagcagag aactccca                                          28


<210> 82
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 82
gaggaccgcc ctgggcctgg aagattcc                                          28


<210> 83
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 83
gtcctcagaa gacaggaggc agcagaga                                          28


<210> 84
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 84
ccctgggcct ggaagattcc cttccccc                                          28


<210> 85
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 85
gcggtcctca gaagacagga ggcagcag                                          28


<210> 86
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 86
tgggcctgga agattccctt cccccttc                                          28


<210> 87
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 87
aagggggaag ggaatcttcc aggcccag                                          28


<210> 88
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 88
ttccctcgtg atctgcaact ggagtctt                                          28


<210> 89
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 89
gggaagaagg gggaagggaa tcttccag                                          28


<210> 90
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 90
gtgatctgca actggagtct ttctggaa                                          28


<210> 91
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 91
cccagctaca gcctcgattt                                                   20


<210> 92
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 92
cacaaatggc gtgttttggt                                                   20
