                         SEQUENCE LISTING

<110>  UNIVERSITE RENE DESCARTES PARIS 5
 
<120>  TUBBY LIKE PROTEIN ISOFORMS AND THEIR APPLICATIONS

<130>  B08831

<160>  13    

<170>  PatentIn version 3.3

<210>  1
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for genrating TUB506-Flag having a Kozak sequence

<400>  1
gcccaccatg acttccaagc ccgcattc                                        28


<210>  2
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for generating TUB506-Flag

<400>  2
gtccttgtaa tcctcgcacg ccagcttgct                                      30


<210>  3
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for generating TUB506-Flag

<400>  3
cttgtcatcg tccccccccc ccccgtcctt gtaatcctcg ca                        42


<210>  4
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for generating TUB506-Flag + TUB561-Flag and 3 
       prime end of TUB561 A277P with a Mlu I site

<400>  4
ctaacgcgtc tacttgtcat cgtcgtcctt gtaatc                               36


<210>  5
<211>  1535
<212>  DNA
<213>  Artificial

<220>
<223>  CDNA sequence of TUB506-Flag

<400>  5
atgacttcca agccgcattc cgactggatt ccctacagtg tcttagatga tgagggcaga     60

aacctgaggc agcagaagct tgatcggcag cgggccctgc tggagcagaa gcagaagaag    120

aagcgccgga gcccctgatg gtgcaggcca atgcagatgg gcggccccgg agccggcggg    180

cccggcagtc agaggaacaa gcccccctgg tggagtccta cctcagcagc agtggcagca    240

ccagctacca agttcaagag gccgactcat cgccagtgtg cagctgggag ccacgcgccc    300

aacagcacca gcttcagcca agagaaccaa ggcggcagct acagcagggg gccagggtgg    360

cgccgctagg aaggagaaga agggaaagca caaaggcacc agcgggccag agcactggca    420

gaagacaagt ctgaggccca aggcccagtg cagattctga ctgtgggcca gtcagaccac    480

gcccaggacg caggggagac ggcagctggt gggggcgaac ggcccagcgg gcaggatctc    540

cgtgccacat gcagaggaag ggcatctcca gcagcatgag ctttgacgag gatgaggagg    600

atgaggagga gaatagctcc agctcctccc agctaaatag taacacccgc cccagctctg    660

ctactagcag gaagtccgtc agggaggcgc ctcagcccct agcccaacag ctccagagca    720

accagtggac gttgaggtcc aggatcttga ggagtttgca ctgaggccgg ccccccaggg    780

tatcaccatc aaatgccgca tcactcggga caagaaaggg atggaccggg gatgtacccc    840

acctactttc tgcacctgga ccgtgaggat gggaagaagg tgttcctcct ggcgggaagg    900

aagagaaaga agagtaaaac ttccaattac ctcatctctg tggacccaac agacttgtct    960

cgaggagggg acagtatatc gggaaactgc ggtccaactt gatgggcacc aagttcactg   1020

tttatgacaa tggagtcaac cctcagaagg cctcatcctc cactttggaa agtggaacct   1080

tacgtcagga gctggcagct gtgtgctacg agacaaagtc ttaggcttca aggggcctcg   1140

gaagatgagc gtgattgtcc caggcatgaa catggttcat gagagagtct ctatccgccc   1200

ccgcaacgag catgagacac tgctagcacg ctggcagaat aagaacacgg agagtatcat   1260

cggctgcaaa acaagacacc tgtctggaat gatgacacac agtcctatgt actcaacttc   1320

catgggcgcg tcacacaggc ctccgtgaag aacttccaga tcatccatgg caatgacccg   1380

gactacatcg tgatgcagtt tggcgggtag cagaggatgt gttcaccatg gattacaact   1440

acccgctgtg tgcactgcag gcctttgcca ttgccctgtc cagcttcgac agcaagctgg   1500

cgtgcgagga ttacaaggac gacgatgaca agtag                              1535


<210>  6
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for generating TUB561-Flag TUB561 A277P having a 
       kozak sequence

<400>  6
gcccaccatg ggggccagga cacct                                           25


<210>  7
<211>  1717
<212>  DNA
<213>  Artificial

<220>
<223>  CDNA sequence of TUB561-Flag

<400>  7
atgggggcca ggacaccttt gccttctttc tgggtttctt tctttgccga gacagggatt     60

ttgttcccag gaggcactcc ctggcccatg ggatctcagc attcaaagca gcacaggaaa    120

cctgggccct gaaacggggc caccgaagag atcggagaac aaccaggagg aagtactgga    180

aggaaggaag ggagatcgct cgtgtcttag atgatgaggg cagaaacctg aggcagcaga    240

agcttgatcg gcagcgggcc ctgctggagc agagcagaag aagaagcgcc aggagcccct    300

gatggtgcag gccaatgcag atgggcggcc ccggagccgg cgggcccggc agtcagagga    360

acaagccccc ctggtggagt cctacctcag cagcagtggc agcaccagct acaagttcaa    420

gaggccgact cactcgccag tgtgcagctg ggagccacgc gcccaacagc accagcttca    480

gccaagagaa ccaaggcggc agctacagca gggggccagg gtggcgccgc taggaaggag    540

aagaagggaa agccaaaggc accagcgggc cagcagcact ggcagaagac aagtctgagg    600

cccaaggccc agtgcagatt ctgactgtgg gccagtcaga ccacgcccag gacgcagggg    660

agacggcagc tggtgggggc gaacggccca gcggcaggat ctccgtgcca cgatgcagag    720

gaagggcatc tccagcagca tgagctttga cgaggatgag gaggatgagg aggagaatag    780

ctccagctcc tcccagctaa atagtaacac ccgccccagc tctgctacta gcggaagtcc    840

gtcagggagg cagcctcagc ccctagccca acagctccag agcaaccagt ggacgttgag    900

gtccaggatc ttgaggagtt tgcactgagg ccggcccccc agggtatcac catcaaatgc    960

cgcatcactc gggaaagaaa gggatggacc ggggcatgta ccccacctac tttctgcacc   1020

tggaccgtga ggatgggaag aaggtgttcc tcctggcggg aaggaagaga aagaagagta   1080

aaacttccaa ttacctcatc tctgtggacc caacagcttg tctcgaggag gggacagcta   1140

tatcgggaaa ctgcggtcca acttgatggg caccaagttc actgtttatg acaatggagt   1200

caaccctcag aaggcctcat cctccacttt ggaaagtgga accttacgtc aggagctggc   1260

gctgtgtgct acgagacaaa cgtcttaggc ttcaaggggc ctcggaagat gagcgtgatt   1320

gtcccaggca tgaacatggt tcatgagaga gtctctatcc gcccccgcaa cgagcatgag   1380

acactgctag cacgctggca gaatagaaca cggagagtat catcgagctg caaaacaaga   1440

cacctgtctg gaatgatgac acacagtcct atgtactcaa cttccatggg cgcgtcacac   1500

aggcctccgt gaagaacttc cagatcatcc atggcaatga cccggactca tcgtgatgca   1560

gtttggccgg gtagcagagg atgtgttcac catggattac aactacccgc tgtgtgcact   1620

gcaggccttt gccattgccc tgtccagctt cgacagcaag ctggcgtgcg aggtcgacgg   1680

atccggtacc gattacaagg acgacgatga caagtag                            1717


<210>  8
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  reverse primer for generating TUB561 A277P

<400>  8
tcctgctagt aggagagctg gggcggg                                         27


<210>  9
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for generating TUB561-Flag 3 prime

<400>  9
agtccgtcag ggaggcagcc tcagc                                           25


<210>  10
<211>  1717
<212>  DNA
<213>  Artificial

<220>
<223>  CDNA of TUB561-Flag A277P

<400>  10
atgggggcca ggacaccttt gccttctttc tgggtttctt tctttgccga gacagggatt     60

ttgttcccag gaggcactcc ctggcccatg ggatctcagc attcaaagca gcacaggaaa    120

cctgggccct gaaacggggc caccgaagag atcggagaac aaccaggagg aagtactgga    180

aggaaggaag ggagatcgct cgtgtcttag atgatgaggg cagaaacctg aggcagcaga    240

agcttgatcg gcagcgggcc ctgctggagc agagcagaag aagaagcgcc aggagcccct    300

gatggtgcag gccaatgcag atgggcggcc ccggagccgg cgggcccggc agtcagagga    360

acaagccccc ctggtggagt cctacctcag cagcagtggc agcaccagct acaagttcaa    420

gaggccgact cactcgccag tgtgcagctg ggagccacgc gcccaacagc accagcttca    480

gccaagagaa ccaaggcggc agctacagca gggggccagg gtggcgccgc taggaaggag    540

aagaagggaa agccaaaggc accagcgggc cagcagcact ggcagaagac aagtctgagg    600

cccaaggccc agtgcagatt ctgactgtgg gccagtcaga ccacgcccag gacgcagggg    660

agacggcagc tggtgggggc gaacggccca gcggcaggat ctccgtgcca cgatgcagag    720

gaagggcatc tccagcagca tgagctttga cgaggatgag gaggatgagg aggagaatag    780

ctccagctcc tcccagctaa atagtaacac ccgccccagc tctcctacta gcggaagtcc    840

gtcagggagg cagcctcagc ccctagccca acagctccag agcaaccagt ggacgttgag    900

gtccaggatc ttgaggagtt tgcactgagg ccggcccccc agggtatcac catcaaatgc    960

cgcatcactc gggaaagaaa gggatggacc ggggcatgta ccccacctac tttctgcacc   1020

tggaccgtga ggatgggaag aaggtgttcc tcctggcggg aaggaagaga aagaagagta   1080

aaacttccaa ttacctcatc tctgtggacc caacagcttg tctcgaggag gggacagcta   1140

tatcgggaaa ctgcggtcca acttgatggg caccaagttc actgtttatg acaatggagt   1200

caaccctcag aaggcctcat cctccacttt ggaaagtgga accttacgtc aggagctggc   1260

gctgtgtgct acgagacaaa cgtcttaggc ttcaaggggc ctcggaagat gagcgtgatt   1320

gtcccaggca tgaacatggt tcatgagaga gtctctatcc gcccccgcaa cgagcatgag   1380

acactgctag cacgctggca gaatagaaca cggagagtat catcgagctg caaaacaaga   1440

cacctgtctg gaatgatgac acacagtcct atgtactcaa cttccatggg cgcgtcacac   1500

aggcctccgt gaagaacttc cagatcatcc atggcaatga cccggactca tcgtgatgca   1560

gtttggccgg gtagcagagg atgtgttcac catggattac aactacccgc tgtgtgcact   1620

gcaggccttt gccattgccc tgtccagctt cgacagcaag ctggcgtgcg aggtcgacgg   1680

atccggtacc gattacaagg acgacgatga caagtag                            1717


<210>  11
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  flag nucleotide sequence

<400>  11
gattacaagg acgacgatga caag                                            24


<210>  12
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  annealed linkers for ligation

<400>  12
gcggtgaccc gggagatctg aattc                                           25


<210>  13
<211>  11
<212>  DNA
<213>  Artificial

<220>
<223>  annealed linkers for ligation

<400>  13
gaattcagat c                                                          11


