                         SEQUENCE LISTING

<110>  Charite Universittsmedizin Berlin
 
<120>  The use of a genetically modified cell line expressing functional
        ASGPR protein in the production of highly sialylated 
       glycoproteins

<130>  P07767WO

<150>  EP 10 162 184.5
<151>  2010-05-06

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  291
<212>  PRT
<213>  homo sapiens

<400>  1

Met Thr Lys Glu Tyr Gln Asp Leu Gln His Leu Asp Asn Glu Glu Ser 
1               5                   10                  15      


Asp His His Gln Leu Arg Lys Gly Pro Pro Pro Pro Gln Pro Leu Leu 
            20                  25                  30          


Gln Arg Leu Cys Ser Gly Pro Arg Leu Leu Leu Leu Ser Leu Gly Leu 
        35                  40                  45              


Ser Leu Leu Leu Leu Val Val Val Cys Val Ile Gly Ser Gln Asn Ser 
    50                  55                  60                  


Gln Leu Gln Glu Glu Leu Arg Gly Leu Arg Glu Thr Phe Ser Asn Phe 
65                  70                  75                  80  


Thr Ala Ser Thr Glu Ala Gln Val Lys Gly Leu Ser Thr Gln Gly Gly 
                85                  90                  95      


Asn Val Gly Arg Lys Met Lys Ser Leu Glu Ser Gln Leu Glu Lys Gln 
            100                 105                 110         


Gln Lys Asp Leu Ser Glu Asp His Ser Ser Leu Leu Leu His Val Lys 
        115                 120                 125             


Gln Phe Val Ser Asp Leu Arg Ser Leu Ser Cys Gln Met Ala Ala Leu 
    130                 135                 140                 


Gln Gly Asn Gly Ser Glu Arg Thr Cys Cys Pro Val Asn Trp Val Glu 
145                 150                 155                 160 


His Glu Arg Ser Cys Tyr Trp Phe Ser Arg Ser Gly Lys Ala Trp Ala 
                165                 170                 175     


Asp Ala Asp Asn Tyr Cys Arg Leu Glu Asp Ala His Leu Val Val Val 
            180                 185                 190         


Thr Ser Trp Glu Glu Gln Lys Phe Val Gln His His Ile Gly Pro Val 
        195                 200                 205             


Asn Thr Trp Met Gly Leu His Asp Gln Asn Gly Pro Trp Lys Trp Val 
    210                 215                 220                 


Asp Gly Thr Asp Tyr Glu Thr Gly Phe Lys Asn Trp Arg Pro Glu Gln 
225                 230                 235                 240 


Pro Asp Asp Trp Tyr Gly His Gly Leu Gly Gly Gly Glu Asp Cys Ala 
                245                 250                 255     


His Phe Thr Asp Asp Gly Arg Trp Asn Asp Asp Val Cys Gln Arg Pro 
            260                 265                 270         


Tyr Arg Trp Val Cys Glu Thr Glu Leu Asp Lys Ala Ser Gln Glu Pro 
        275                 280                 285             


Pro Leu Leu 
    290     


<210>  2
<211>  311
<212>  PRT
<213>  homo sapiens

<400>  2

Met Ala Lys Asp Phe Gln Asp Ile Gln Gln Leu Ser Ser Glu Glu Asn 
1               5                   10                  15      


Asp His Pro Phe His Gln Gly Glu Gly Pro Gly Thr Arg Arg Leu Asn 
            20                  25                  30          


Pro Arg Arg Gly Asn Pro Phe Leu Lys Gly Pro Pro Pro Ala Gln Pro 
        35                  40                  45              


Leu Ala Gln Arg Leu Cys Ser Met Val Cys Phe Ser Leu Leu Ala Leu 
    50                  55                  60                  


Ser Phe Asn Ile Leu Leu Leu Val Val Ile Cys Val Thr Gly Ser Gln 
65                  70                  75                  80  


Ser Glu Gly His Arg Gly Ala Gln Leu Gln Ala Glu Leu Arg Ser Leu 
                85                  90                  95      


Lys Glu Ala Phe Ser Asn Phe Ser Ser Ser Thr Leu Thr Glu Val Gln 
            100                 105                 110         


Ala Ile Ser Thr His Gly Gly Ser Val Gly Asp Lys Ile Thr Ser Leu 
        115                 120                 125             


Gly Ala Lys Leu Glu Lys Gln Gln Gln Asp Leu Lys Ala Asp His Asp 
    130                 135                 140                 


Ala Leu Leu Phe His Leu Lys His Phe Pro Val Asp Leu Arg Phe Val 
145                 150                 155                 160 


Ala Cys Gln Met Glu Leu Leu His Ser Asn Gly Ser Gln Arg Thr Cys 
                165                 170                 175     


Cys Pro Val Asn Trp Val Glu His Gln Gly Ser Cys Tyr Trp Phe Ser 
            180                 185                 190         


His Ser Gly Lys Ala Trp Ala Glu Ala Glu Lys Tyr Cys Gln Leu Glu 
        195                 200                 205             


Asn Ala His Leu Val Val Ile Asn Ser Trp Glu Glu Gln Lys Phe Ile 
    210                 215                 220                 


Val Gln His Thr Asn Pro Phe Asn Thr Trp Ile Gly Leu Thr Asp Ser 
225                 230                 235                 240 


Asp Gly Ser Trp Lys Trp Val Asp Gly Thr Asp Tyr Arg His Asn Tyr 
                245                 250                 255     


Lys Asn Trp Ala Val Thr Gln Pro Asp Asn Trp His Gly His Glu Leu 
            260                 265                 270         


Gly Gly Ser Glu Asp Cys Val Glu Val Gln Pro Asp Gly Arg Trp Asn 
        275                 280                 285             


Asp Asp Phe Cys Leu Gln Val Tyr Arg Trp Val Cys Glu Lys Arg Arg 
    290                 295                 300                 


Asn Ala Thr Gly Glu Val Ala 
305                 310     


<210>  3
<211>  876
<212>  DNA
<213>  homo sapiens

<400>  3
atgaccaagg agtatcaaga ccttcagcat ctggacaatg aggagagtga ccaccatcag       60

ctcagaaaag ggccacctcc tccccagccc ctcctgcagc gtctctgctc cggacctcgc      120

ctcctcctgc tctccctggg cctcagcctc ctgctgcttg tggttgtctg tgtgatcgga      180

tcccaaaact cccagctgca ggaggagctg cggggcctga gagagacgtt cagcaacttc      240

acagcgagca cggaggccca ggtcaagggc ttgagcaccc agggaggcaa tgtgggaaga      300

aagatgaagt cgctagagtc ccagctggag aaacagcaga aggacctgag tgaagatcac      360

tccagcctgc tgctccacgt gaagcagttc gtgtctgacc tgcggagcct gagctgtcag      420

atggcggcgc tccagggcaa tggctcagaa aggacctgct gcccggtcaa ctgggtggag      480

cacgagcgca gctgctactg gttctctcgc tccgggaagg cctgggctga cgccgacaac      540

tactgccggc tggaggacgc gcacctggtg gtggtcacgt cctgggagga gcagaaattt      600

gtccagcacc acataggccc tgtgaacacc tggatgggcc tccacgacca aaacgggccc      660

tggaagtggg tggacgggac ggactacgag acgggcttca agaactggag gccggagcag      720

ccggacgact ggtacggcca cgggctcgga ggaggcgagg actgtgccca cttcaccgac      780

gacggccgct ggaacgacga cgtctgccag aggccctacc gctgggtctg cgagacagag      840

ctggacaagg ccagccagga gccacctctc ctttaa                                876


<210>  4
<211>  936
<212>  DNA
<213>  homo sapiens

<400>  4
atggccaagg actttcaaga tatccagcag ctgagctcgg aggaaaatga ccatcctttc       60

catcaaggtg aggggccagg cactcgcagg ctgaatccca ggagaggaaa tccatttttg      120

aaagggccac ctcctgccca gcccctggca cagcgtctct gctccatggt ctgcttcagt      180

ctgcttgccc tgagcttcaa catcctgctg ctggtggtca tctgtgtgac tgggtcccaa      240

agtgagggtc acagaggtgc acagctgcaa gccgagctgc ggagcctgaa ggaagctttc      300

agcaacttct cctcgagcac cctgacggag gtccaggcaa tcagcaccca cggaggcagc      360

gtgggtgaca agatcacatc cctaggagcc aagctggaga aacagcagca ggacctgaaa      420

gcagatcacg atgccctgct cttccatctg aagcacttcc ccgtggacct gcgcttcgtg      480

gcctgccaga tggagctcct ccacagcaac ggctcccaaa ggacctgctg ccccgtcaac      540

tgggtggagc accaaggcag ctgctactgg ttctctcact ccgggaaggc ctgggctgag      600

gcggagaagt actgccagct ggagaacgca cacctggtgg tcatcaactc ctgggaggag      660

cagaaattca ttgtacaaca cacgaacccc ttcaatacct ggataggtct cacggacagt      720

gatggctctt ggaaatgggt ggatggcaca gactataggc acaactacaa gaactgggct      780

gtcactcagc cagataattg gcacgggcac gagctgggtg gaagtgaaga ctgtgttgaa      840

gtccagccgg atggccgctg gaacgatgac ttctgcctgc aggtgtaccg ctgggtgtgt      900

gagaaaaggc ggaatgccac cggcgaggtg gcctga                                936


<210>  5
<211>  287
<212>  PRT
<213>  homo sapiens

<400>  5

Met Ala Lys Asp Phe Gln Asp Ile Gln Gln Leu Ser Ser Glu Glu Asn 
1               5                   10                  15      


Asp His Pro Phe His Gln Gly Pro Pro Pro Ala Gln Pro Leu Ala Gln 
            20                  25                  30          


Arg Leu Cys Ser Met Val Cys Phe Ser Leu Leu Ala Leu Ser Phe Asn 
        35                  40                  45              


Ile Leu Leu Leu Val Val Ile Cys Val Thr Gly Ser Gln Ser Ala Gln 
    50                  55                  60                  


Leu Gln Ala Glu Leu Arg Ser Leu Lys Glu Ala Phe Ser Asn Phe Ser 
65                  70                  75                  80  


Ser Ser Thr Leu Thr Glu Val Gln Ala Ile Ser Thr His Gly Gly Ser 
                85                  90                  95      


Val Gly Asp Lys Ile Thr Ser Leu Gly Ala Lys Leu Glu Lys Gln Gln 
            100                 105                 110         


Gln Asp Leu Lys Ala Asp His Asp Ala Leu Leu Phe His Leu Lys His 
        115                 120                 125             


Phe Pro Val Asp Leu Arg Phe Val Ala Cys Gln Met Glu Leu Leu His 
    130                 135                 140                 


Ser Asn Gly Ser Gln Arg Thr Cys Cys Pro Val Asn Trp Val Glu His 
145                 150                 155                 160 


Gln Gly Ser Cys Tyr Trp Phe Ser His Ser Gly Lys Ala Trp Ala Glu 
                165                 170                 175     


Ala Glu Lys Tyr Cys Gln Leu Glu Asn Ala His Leu Val Val Ile Asn 
            180                 185                 190         


Ser Trp Glu Glu Gln Lys Phe Ile Val Gln His Thr Asn Pro Phe Asn 
        195                 200                 205             


Thr Trp Ile Gly Leu Thr Asp Ser Asp Gly Ser Trp Lys Trp Val Asp 
    210                 215                 220                 


Gly Thr Asp Tyr Arg His Asn Tyr Lys Asn Trp Ala Val Thr Gln Pro 
225                 230                 235                 240 


Asp Asn Trp His Gly His Glu Leu Gly Gly Ser Glu Asp Cys Val Glu 
                245                 250                 255     


Val Gln Pro Asp Gly Arg Trp Asn Asp Asp Phe Cys Leu Gln Val Tyr 
            260                 265                 270         


Arg Trp Val Cys Glu Lys Arg Arg Asn Ala Thr Gly Glu Val Ala 
        275                 280                 285         


<210>  6
<211>  292
<212>  PRT
<213>  homo sapiens

<400>  6

Met Ala Lys Asp Phe Gln Asp Ile Gln Gln Leu Ser Ser Glu Glu Asn 
1               5                   10                  15      


Asp His Pro Phe His Gln Gly Pro Pro Pro Ala Gln Pro Leu Ala Gln 
            20                  25                  30          


Arg Leu Cys Ser Met Val Cys Phe Ser Leu Leu Ala Leu Ser Phe Asn 
        35                  40                  45              


Ile Leu Leu Leu Val Val Ile Cys Val Thr Gly Ser Gln Ser Glu Gly 
    50                  55                  60                  


His Arg Gly Ala Gln Leu Gln Ala Glu Leu Arg Ser Leu Lys Glu Ala 
65                  70                  75                  80  


Phe Ser Asn Phe Ser Ser Ser Thr Leu Thr Glu Val Gln Ala Ile Ser 
                85                  90                  95      


Thr His Gly Gly Ser Val Gly Asp Lys Ile Thr Ser Leu Gly Ala Lys 
            100                 105                 110         


Leu Glu Lys Gln Gln Gln Asp Leu Lys Ala Asp His Asp Ala Leu Leu 
        115                 120                 125             


Phe His Leu Lys His Phe Pro Val Asp Leu Arg Phe Val Ala Cys Gln 
    130                 135                 140                 


Met Glu Leu Leu His Ser Asn Gly Ser Gln Arg Thr Cys Cys Pro Val 
145                 150                 155                 160 


Asn Trp Val Glu His Gln Gly Ser Cys Tyr Trp Phe Ser His Ser Gly 
                165                 170                 175     


Lys Ala Trp Ala Glu Ala Glu Lys Tyr Cys Gln Leu Glu Asn Ala His 
            180                 185                 190         


Leu Val Val Ile Asn Ser Trp Glu Glu Gln Lys Phe Ile Val Gln His 
        195                 200                 205             


Thr Asn Pro Phe Asn Thr Trp Ile Gly Leu Thr Asp Ser Asp Gly Ser 
    210                 215                 220                 


Trp Lys Trp Val Asp Gly Thr Asp Tyr Arg His Asn Tyr Lys Asn Trp 
225                 230                 235                 240 


Ala Val Thr Gln Pro Asp Asn Trp His Gly His Glu Leu Gly Gly Ser 
                245                 250                 255     


Glu Asp Cys Val Glu Val Gln Pro Asp Gly Arg Trp Asn Asp Asp Phe 
            260                 265                 270         


Cys Leu Gln Val Tyr Arg Trp Val Cys Glu Lys Arg Arg Asn Ala Thr 
        275                 280                 285             


Gly Glu Val Ala 
    290         


<210>  7
<211>  864
<212>  DNA
<213>  homo sapiens

<400>  7
atggccaagg actttcaaga tatccagcag ctgagctcgg aggaaaatga ccatcctttc       60

catcaagggc cacctcctgc ccagcccctg gcacagcgtc tctgctccat ggtctgcttc      120

agtctgcttg ccctgagctt caacatcctg ctgctggtgg tcatctgtgt gactgggtcc      180

caaagtgcac agctgcaagc cgagctgcgg agcctgaagg aagctttcag caacttctcc      240

tcgagcaccc tgacggaggt ccaggcaatc agcacccacg gaggcagcgt gggtgacaag      300

atcacatccc taggagccaa gctggagaaa cagcagcagg acctgaaagc agatcacgat      360

gccctgctct tccatctgaa gcacttcccc gtggacctgc gcttcgtggc ctgccagatg      420

gagctcctcc acagcaacgg ctcccaaagg acctgctgcc ccgtcaactg ggtggagcac      480

caaggcagct gctactggtt ctctcactcc gggaaggcct gggctgaggc ggagaagtac      540

tgccagctgg agaacgcaca cctggtggtc atcaactcct gggaggagca gaaattcatt      600

gtacaacaca cgaacccctt caatacctgg ataggtctca cggacagtga tggctcttgg      660

aaatgggtgg atggcacaga ctataggcac aactacaaga actgggctgt cactcagcca      720

gataattggc acgggcacga gctgggtgga agtgaagact gtgttgaagt ccagccggat      780

ggccgctgga acgatgactt ctgcctgcag gtgtaccgct gggtgtgtga gaaaaggcgg      840

aatgccaccg gcgaggtggc ctga                                             864


<210>  8
<211>  879
<212>  DNA
<213>  homo sapiens

<400>  8
atggccaagg actttcaaga tatccagcag ctgagctcgg aggaaaatga ccatcctttc       60

catcaagggc cacctcctgc ccagcccctg gcacagcgtc tctgctccat ggtctgcttc      120

agtctgcttg ccctgagctt caacatcctg ctgctggtgg tcatctgtgt gactgggtcc      180

caaagtgagg gtcacagagg tgcacagctg caagccgagc tgcggagcct gaaggaagct      240

ttcagcaact tctcctcgag caccctgacg gaggtccagg caatcagcac ccacggaggc      300

agcgtgggtg acaagatcac atccctagga gccaagctgg agaaacagca gcaggacctg      360

aaagcagatc acgatgccct gctcttccat ctgaagcact tccccgtgga cctgcgcttc      420

gtggcctgcc agatggagct cctccacagc aacggctccc aaaggacctg ctgccccgtc      480

aactgggtgg agcaccaagg cagctgctac tggttctctc actccgggaa ggcctgggct      540

gaggcggaga agtactgcca gctggagaac gcacacctgg tggtcatcaa ctcctgggag      600

gagcagaaat tcattgtaca acacacgaac cccttcaata cctggatagg tctcacggac      660

agtgatggct cttggaaatg ggtggatggc acagactata ggcacaacta caagaactgg      720

gctgtcactc agccagataa ttggcacggg cacgagctgg gtggaagtga agactgtgtt      780

gaagtccagc cggatggccg ctggaacgat gacttctgcc tgcaggtgta ccgctgggtg      840

tgtgagaaaa ggcggaatgc caccggcgag gtggcctga                             879


<210>  9
<211>  284
<212>  PRT
<213>  Mus musculus

<400>  9

Met Thr Lys Asp Tyr Gln Asp Phe Gln His Leu Asp Asn Asp Asn Asp 
1               5                   10                  15      


His His Gln Leu Arg Arg Gly Pro Pro Pro Thr Pro Arg Leu Leu Gln 
            20                  25                  30          


Arg Leu Cys Ser Gly Ser Arg Leu Leu Leu Leu Ser Ser Ser Leu Ser 
        35                  40                  45              


Ile Leu Leu Leu Val Val Val Cys Val Ile Thr Ser Gln Asn Ser Gln 
    50                  55                  60                  


Leu Arg Glu Asp Leu Leu Ala Leu Arg Gln Asn Phe Ser Asn Leu Thr 
65                  70                  75                  80  


Val Ser Thr Glu Asp Gln Val Lys Ala Leu Ser Thr Gln Gly Ser Ser 
                85                  90                  95      


Val Gly Arg Lys Met Lys Leu Val Glu Ser Lys Leu Glu Lys Gln Gln 
            100                 105                 110         


Lys Asp Leu Thr Glu Asp His Ser Ser Leu Leu Leu His Val Lys Gln 
        115                 120                 125             


Leu Val Ser Asp Val Arg Ser Leu Ser Cys Gln Met Ala Ala Phe Arg 
    130                 135                 140                 


Gly Asn Gly Ser Glu Arg Ile Cys Cys Pro Ile Asn Trp Val Glu Tyr 
145                 150                 155                 160 


Glu Gly Ser Cys Tyr Trp Phe Ser Ser Ser Val Arg Pro Trp Thr Glu 
                165                 170                 175     


Ala Asp Lys Tyr Cys Gln Leu Glu Asn Ala His Leu Val Val Val Thr 
            180                 185                 190         


Ser Arg Asp Glu Gln Asn Phe Leu Gln Arg His Met Gly Pro Leu Asn 
        195                 200                 205             


Thr Trp Ile Gly Leu Thr Asp Gln Asn Gly Pro Trp Lys Trp Val Asp 
    210                 215                 220                 


Gly Thr Asp Tyr Glu Thr Gly Phe Gln Asn Trp Arg Pro Glu Gln Pro 
225                 230                 235                 240 


Asp Asn Trp Tyr Gly His Gly Leu Gly Gly Gly Glu Asp Cys Ala His 
                245                 250                 255     


Phe Thr Thr Asp Gly Arg Trp Asn Asp Asp Val Cys Arg Arg Pro Tyr 
            260                 265                 270         


Arg Trp Val Cys Glu Thr Lys Leu Asp Lys Ala Asn 
        275                 280                 


<210>  10
<211>  284
<212>  PRT
<213>  Rattus norvegicus

<400>  10

Met Thr Lys Asp Tyr Gln Asp Phe Gln His Leu Asp Asn Glu Asn Asp 
1               5                   10                  15      


His His Gln Leu Gln Arg Gly Pro Pro Pro Ala Pro Arg Leu Leu Gln 
            20                  25                  30          


Arg Leu Cys Ser Gly Phe Arg Leu Phe Leu Leu Ser Leu Gly Leu Ser 
        35                  40                  45              


Ile Leu Leu Leu Val Val Val Cys Val Ile Thr Ser Gln Asn Ser Gln 
    50                  55                  60                  


Leu Arg Glu Asp Leu Arg Val Leu Arg Gln Asn Phe Ser Asn Phe Thr 
65                  70                  75                  80  


Val Ser Thr Glu Asp Gln Val Lys Ala Leu Thr Thr Gln Gly Glu Arg 
                85                  90                  95      


Val Gly Arg Lys Met Lys Leu Val Glu Ser Gln Leu Glu Lys His Gln 
            100                 105                 110         


Glu Asp Leu Arg Glu Asp His Ser Arg Leu Leu Leu His Val Lys Gln 
        115                 120                 125             


Leu Val Ser Asp Val Arg Ser Leu Ser Cys Gln Met Ala Ala Leu Arg 
    130                 135                 140                 


Gly Asn Gly Ser Glu Arg Ile Cys Cys Pro Ile Asn Trp Val Glu Tyr 
145                 150                 155                 160 


Glu Gly Ser Cys Tyr Trp Phe Ser Ser Ser Val Lys Pro Trp Thr Glu 
                165                 170                 175     


Ala Asp Lys Tyr Cys Gln Leu Glu Asn Ala His Leu Val Val Val Thr 
            180                 185                 190         


Ser Trp Glu Glu Gln Arg Phe Val Gln Gln His Met Gly Pro Leu Asn 
        195                 200                 205             


Thr Trp Ile Gly Leu Thr Asp Gln Asn Gly Pro Trp Lys Trp Val Asp 
    210                 215                 220                 


Gly Thr Asp Tyr Glu Thr Gly Phe Lys Asn Trp Arg Pro Gly Gln Pro 
225                 230                 235                 240 


Asp Asp Trp Tyr Gly His Gly Leu Gly Gly Gly Glu Asp Cys Ala His 
                245                 250                 255     


Phe Thr Thr Asp Gly His Trp Asn Asp Asp Val Cys Arg Arg Pro Tyr 
            260                 265                 270         


Arg Trp Val Cys Glu Thr Glu Leu Gly Lys Ala Asn 
        275                 280                 


<210>  11
<211>  301
<212>  PRT
<213>  Mus musculus

<400>  11

Met Glu Lys Asp Cys Gln Asp Ile Gln Gln Leu Asp Ser Glu Glu Asn 
1               5                   10                  15      


Asp His Gln Leu Ser Gly Asp Asp Glu His Gly Ser His Val Gln Asp 
            20                  25                  30          


Pro Arg Ile Glu Asn Pro His Trp Lys Gly Gln Pro Leu Ser Arg Pro 
        35                  40                  45              


Phe Pro Gln Arg Leu Cys Ser Thr Phe Arg Leu Ser Leu Leu Ala Leu 
    50                  55                  60                  


Ala Phe Asn Ile Leu Leu Leu Val Val Ile Cys Val Val Ser Ser Gln 
65                  70                  75                  80  


Ser Ile Gln Leu Gln Glu Glu Phe Arg Thr Leu Lys Glu Thr Phe Ser 
                85                  90                  95      


Asn Phe Ser Ser Ser Thr Leu Met Glu Phe Gly Ala Leu Asp Thr Leu 
            100                 105                 110         


Gly Gly Ser Thr Asn Ala Ile Leu Thr Ser Trp Leu Ala Gln Leu Glu 
        115                 120                 125             


Glu Lys Gln Gln Gln Leu Lys Ala Asp His Ser Thr Leu Leu Phe His 
    130                 135                 140                 


Leu Lys His Phe Pro Met Asp Leu Arg Thr Leu Thr Cys Gln Leu Ala 
145                 150                 155                 160 


Tyr Phe Gln Ser Asn Gly Thr Glu Cys Cys Pro Val Asn Trp Val Glu 
                165                 170                 175     


Phe Gly Gly Ser Cys Tyr Trp Phe Ser Arg Asp Gly Leu Thr Trp Ala 
            180                 185                 190         


Glu Ala Asp Gln Tyr Cys Gln Leu Glu Asn Ala His Leu Leu Val Ile 
        195                 200                 205             


Asn Ser Arg Glu Glu Gln Asp Phe Val Val Lys His Arg Ser Gln Phe 
    210                 215                 220                 


His Ile Trp Ile Gly Leu Thr Asp Arg Asp Gly Ser Trp Lys Trp Val 
225                 230                 235                 240 


Asp Gly Thr Asp Tyr Arg Ser Asn Tyr Arg Asn Trp Ala Phe Thr Gln 
                245                 250                 255     


Pro Asp Asn Trp Gln Gly His Glu Gln Gly Gly Gly Glu Asp Cys Ala 
            260                 265                 270         


Glu Ile Leu Ser Asp Gly His Trp Asn Asp Asn Phe Cys Gln Gln Val 
        275                 280                 285             


Asn Arg Trp Val Cys Glu Lys Arg Arg Asn Ile Thr His 
    290                 295                 300     


<210>  12
<211>  301
<212>  PRT
<213>  Rattus norvegicus

<400>  12

Met Glu Lys Asp Phe Gln Asp Ile Gln Gln Leu Asp Ser Glu Glu Asn 
1               5                   10                  15      


Asp His Gln Leu Ile Gly Asp Glu Glu Gln Gly Ser His Val Gln Asn 
            20                  25                  30          


Leu Arg Thr Glu Asn Pro Arg Trp Gly Gly Gln Pro Pro Ser Arg Pro 
        35                  40                  45              


Phe Pro Gln Arg Leu Cys Ser Lys Phe Arg Leu Ser Leu Leu Ala Leu 
    50                  55                  60                  


Ala Phe Asn Ile Leu Leu Leu Val Val Ile Cys Val Val Ser Ser Gln 
65                  70                  75                  80  


Ser Met Gln Leu Gln Lys Glu Phe Trp Thr Leu Lys Glu Thr Leu Ser 
                85                  90                  95      


Asn Phe Ser Thr Thr Thr Leu Met Glu Phe Lys Ala Leu Asp Ser His 
            100                 105                 110         


Gly Gly Ser Arg Asn Asp Asn Leu Thr Ser Trp Glu Thr Ile Leu Glu 
        115                 120                 125             


Lys Lys Gln Lys Asp Ile Lys Ala Asp His Ser Thr Leu Leu Phe His 
    130                 135                 140                 


Leu Lys His Phe Pro Leu Asp Leu Arg Thr Leu Thr Cys Gln Leu Ala 
145                 150                 155                 160 


Phe Phe Leu Ser Asn Gly Thr Glu Cys Cys Pro Val Asn Trp Val Glu 
                165                 170                 175     


Phe Gly Gly Ser Cys Tyr Trp Phe Ser Arg Asp Gly Leu Thr Trp Ala 
            180                 185                 190         


Glu Ala Asp Gln Tyr Cys Gln Met Glu Asn Ala His Leu Leu Val Ile 
        195                 200                 205             


Asn Ser Arg Glu Glu Gln Glu Phe Val Val Lys His Arg Gly Ala Phe 
    210                 215                 220                 


His Ile Trp Ile Gly Leu Thr Asp Lys Asp Gly Ser Trp Lys Trp Val 
225                 230                 235                 240 


Asp Gly Thr Glu Tyr Arg Ser Asn Phe Lys Asn Trp Ala Phe Thr Gln 
                245                 250                 255     


Pro Asp Asn Trp Gln Gly His Glu Glu Gly Gly Ser Glu Asp Cys Ala 
            260                 265                 270         


Glu Ile Leu Ser Asp Gly Leu Trp Asn Asp Asn Phe Cys Gln Gln Val 
        275                 280                 285             


Asn Arg Trp Ala Cys Glu Arg Lys Arg Asp Ile Thr Tyr 
    290                 295                 300     


