                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
       Paulovich, A.G.
       Ivey, R.G.
 
<120>  Compositions and Methods for Reliably Detecting and/or Measuring 
       the Amount of a Modified Target Protein in a Sample

<130>  FHCR-1-36764

<150>  US 61/330,278
<151>  2010-04-30

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  1

Asp Leu Thr Lys Tyr Pro Asp Ala Asn Pro Asn Pro Asn Glu Gln 
1               5                   10                  15  


<210>  2
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Wherein the Serine at position 7 can be phosphorylated Serine

<400>  2

Ser Gln Glu Glu Gly Ser Ser Gln Gly Glu Asp Ser Val Ser Gly 
1               5                   10                  15  


<210>  3
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Wherein the Serine at position 6 can be phosphorylated Serine

<400>  3

Asp Ser Val Ser Gly Ser Gln Arg Ile Ser Ser 
1               5                   10      


<210>  4
<211>  28
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Wherein the Serine at position 8 can be phosphorylated Serine

<400>  4

Ile Ser Gln Glu Glu Gly Ser Ser Gln Gly Glu Asp Ser Asp Leu Thr 
1               5                   10                  15      


Lys Tyr Pro Asp Ala Asn Pro Asn Pro Asn Glu Gln 
            20                  25              


<210>  5
<211>  28
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Wherein the Serine at position 7 can be phosphorylated Serine

<400>  5

Glu Asp Ser Val Ser Gly Ser Gln Arg Ile Ser Ser Ile Asp Leu Thr 
1               5                   10                  15      


Lys Tyr Pro Asp Ala Asn Pro Asn Pro Asn Glu Gln 
            20                  25              


<210>  6
<211>  1233
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Gly Phe Leu Lys Leu Ile Glu Ile Glu Asn Phe Lys Ser Tyr Lys 
1               5                   10                  15      


Gly Arg Gln Ile Ile Gly Pro Phe Gln Arg Phe Thr Ala Ile Ile Gly 
            20                  25                  30          


Pro Asn Gly Ser Gly Lys Ser Asn Leu Met Asp Ala Ile Ser Phe Val 
        35                  40                  45              


Leu Gly Glu Lys Thr Ser Asn Leu Arg Val Lys Thr Leu Arg Asp Leu 
    50                  55                  60                  


Ile His Gly Ala Pro Val Gly Lys Pro Ala Ala Asn Arg Ala Phe Val 
65                  70                  75                  80  


Ser Met Val Tyr Ser Glu Glu Gly Ala Glu Asp Arg Thr Phe Ala Arg 
                85                  90                  95      


Val Ile Val Gly Gly Ser Ser Glu Tyr Lys Ile Asn Asn Lys Val Val 
            100                 105                 110         


Gln Leu His Glu Tyr Ser Glu Glu Leu Glu Lys Leu Gly Ile Leu Ile 
        115                 120                 125             


Lys Ala Arg Asn Phe Leu Val Phe Gln Gly Ala Val Glu Ser Ile Ala 
    130                 135                 140                 


Met Lys Asn Pro Lys Glu Arg Thr Ala Leu Phe Glu Glu Ile Ser Arg 
145                 150                 155                 160 


Ser Gly Glu Leu Ala Gln Glu Tyr Asp Lys Arg Lys Lys Glu Met Val 
                165                 170                 175     


Lys Ala Glu Glu Asp Thr Gln Phe Asn Tyr His Arg Lys Lys Asn Ile 
            180                 185                 190         


Ala Ala Glu Arg Lys Glu Ala Lys Gln Glu Lys Glu Glu Ala Asp Arg 
        195                 200                 205             


Tyr Gln Arg Leu Lys Asp Glu Val Val Arg Ala Gln Val Gln Leu Gln 
    210                 215                 220                 


Leu Phe Lys Leu Tyr His Asn Glu Val Glu Ile Glu Lys Leu Asn Lys 
225                 230                 235                 240 


Glu Leu Ala Ser Lys Asn Lys Glu Ile Glu Lys Asp Lys Lys Arg Met 
                245                 250                 255     


Asp Lys Val Glu Asp Glu Leu Lys Glu Lys Lys Lys Glu Leu Gly Lys 
            260                 265                 270         


Met Met Arg Glu Gln Gln Gln Ile Glu Lys Glu Ile Lys Glu Lys Asp 
        275                 280                 285             


Ser Glu Leu Asn Gln Lys Arg Pro Gln Tyr Ile Lys Ala Lys Glu Asn 
    290                 295                 300                 


Thr Ser His Lys Ile Lys Lys Leu Glu Ala Ala Lys Lys Ser Leu Gln 
305                 310                 315                 320 


Asn Ala Gln Lys His Tyr Lys Lys Arg Lys Gly Asp Met Asp Glu Leu 
                325                 330                 335     


Glu Lys Glu Met Leu Ser Val Glu Lys Ala Arg Gln Glu Phe Glu Glu 
            340                 345                 350         


Arg Met Glu Glu Glu Ser Gln Ser Gln Gly Arg Asp Leu Thr Leu Glu 
        355                 360                 365             


Glu Asn Gln Val Lys Lys Tyr His Arg Leu Lys Glu Glu Ala Ser Lys 
    370                 375                 380                 


Arg Ala Ala Thr Leu Ala Gln Glu Leu Glu Lys Phe Asn Arg Asp Gln 
385                 390                 395                 400 


Lys Ala Asp Gln Asp Arg Leu Asp Leu Glu Glu Arg Lys Lys Val Glu 
                405                 410                 415     


Thr Glu Ala Lys Ile Lys Gln Lys Leu Arg Glu Ile Glu Glu Asn Gln 
            420                 425                 430         


Lys Arg Ile Glu Lys Leu Glu Glu Tyr Ile Thr Thr Ser Lys Gln Ser 
        435                 440                 445             


Leu Glu Glu Gln Lys Lys Leu Glu Gly Glu Leu Thr Glu Glu Val Glu 
    450                 455                 460                 


Met Ala Lys Arg Arg Ile Asp Glu Ile Asn Lys Glu Leu Asn Gln Val 
465                 470                 475                 480 


Met Glu Gln Leu Gly Asp Ala Arg Ile Asp Arg Gln Glu Ser Ser Arg 
                485                 490                 495     


Gln Gln Arg Lys Ala Glu Ile Met Glu Ser Ile Lys Arg Leu Tyr Pro 
            500                 505                 510         


Gly Ser Val Tyr Gly Arg Leu Ile Asp Leu Cys Gln Pro Thr Gln Lys 
        515                 520                 525             


Lys Tyr Gln Ile Ala Val Thr Lys Val Leu Gly Lys Asn Met Asp Ala 
    530                 535                 540                 


Ile Ile Val Asp Ser Glu Lys Thr Gly Arg Asp Cys Ile Gln Tyr Ile 
545                 550                 555                 560 


Lys Glu Gln Arg Gly Glu Pro Glu Thr Phe Leu Pro Leu Asp Tyr Leu 
                565                 570                 575     


Glu Val Lys Pro Thr Asp Glu Lys Leu Arg Glu Leu Lys Gly Ala Lys 
            580                 585                 590         


Leu Val Ile Asp Val Ile Arg Tyr Glu Pro Pro His Ile Lys Lys Ala 
        595                 600                 605             


Leu Gln Tyr Ala Cys Gly Asn Ala Leu Val Cys Asp Asn Val Glu Asp 
    610                 615                 620                 


Ala Arg Arg Ile Ala Phe Gly Gly His Gln Arg His Lys Thr Val Ala 
625                 630                 635                 640 


Leu Asp Gly Thr Leu Phe Gln Lys Ser Gly Val Ile Ser Gly Gly Ala 
                645                 650                 655     


Ser Asp Leu Lys Ala Lys Ala Arg Arg Trp Asp Glu Lys Ala Val Asp 
            660                 665                 670         


Lys Leu Lys Glu Lys Lys Glu Arg Leu Thr Glu Glu Leu Lys Glu Gln 
        675                 680                 685             


Met Lys Ala Lys Arg Lys Glu Ala Glu Leu Arg Gln Val Gln Ser Gln 
    690                 695                 700                 


Ala His Gly Leu Gln Met Arg Leu Lys Tyr Ser Gln Ser Asp Leu Glu 
705                 710                 715                 720 


Gln Thr Lys Thr Arg His Leu Ala Leu Asn Leu Gln Glu Lys Ser Lys 
                725                 730                 735     


Leu Glu Ser Glu Leu Ala Asn Phe Gly Pro Arg Ile Asn Asp Ile Lys 
            740                 745                 750         


Arg Ile Ile Gln Ser Arg Glu Arg Glu Met Lys Asp Leu Lys Glu Lys 
        755                 760                 765             


Met Asn Gln Val Glu Asp Glu Val Phe Glu Glu Phe Cys Arg Glu Ile 
    770                 775                 780                 


Gly Val Arg Asn Ile Arg Glu Phe Glu Glu Glu Lys Val Lys Arg Gln 
785                 790                 795                 800 


Asn Glu Ile Ala Lys Lys Arg Leu Glu Phe Glu Asn Gln Lys Thr Arg 
                805                 810                 815     


Leu Gly Ile Gln Leu Asp Phe Glu Lys Asn Gln Leu Lys Glu Asp Gln 
            820                 825                 830         


Asp Lys Val His Met Trp Glu Gln Thr Val Lys Lys Asp Glu Asn Glu 
        835                 840                 845             


Ile Glu Lys Leu Lys Lys Glu Glu Gln Arg His Met Lys Ile Ile Asp 
    850                 855                 860                 


Glu Thr Met Ala Gln Leu Gln Asp Leu Lys Asn Gln His Leu Ala Lys 
865                 870                 875                 880 


Lys Ser Glu Val Asn Asp Lys Asn His Glu Met Glu Glu Ile Arg Lys 
                885                 890                 895     


Lys Leu Gly Gly Ala Asn Lys Glu Met Thr His Leu Gln Lys Glu Val 
            900                 905                 910         


Thr Ala Ile Glu Thr Lys Leu Glu Gln Lys Arg Ser Asp Arg His Asn 
        915                 920                 925             


Leu Leu Gln Ala Cys Lys Met Gln Asp Ile Lys Leu Pro Leu Ser Lys 
    930                 935                 940                 


Gly Thr Met Asp Asp Ile Ser Gln Glu Glu Gly Ser Ser Gln Gly Glu 
945                 950                 955                 960 


Asp Ser Val Ser Gly Ser Gln Arg Ile Ser Ser Ile Tyr Ala Arg Glu 
                965                 970                 975     


Ala Leu Ile Glu Ile Asp Tyr Gly Asp Leu Cys Glu Asp Leu Lys Asp 
            980                 985                 990         


Ala Gln Ala Glu Glu Glu Ile Lys  Gln Glu Met Asn Thr  Leu Gln Gln 
        995                 1000                 1005             


Lys Leu  Asn Glu Gln Gln Ser  Val Leu Gln Arg Ile  Ala Ala Pro 
    1010                 1015                 1020             


Asn Met  Lys Ala Met Glu Lys  Leu Glu Ser Val Arg  Asp Lys Phe 
    1025                 1030                 1035             


Gln Glu  Thr Ser Asp Glu Phe  Glu Ala Ala Arg Lys  Arg Ala Lys 
    1040                 1045                 1050             


Lys Ala  Lys Gln Ala Phe Glu  Gln Ile Lys Lys Glu  Arg Phe Asp 
    1055                 1060                 1065             


Arg Phe  Asn Ala Cys Phe Glu  Ser Val Ala Thr Asn  Ile Asp Glu 
    1070                 1075                 1080             


Ile Tyr  Lys Ala Leu Ser Arg  Asn Ser Ser Ala Gln  Ala Phe Leu 
    1085                 1090                 1095             


Gly Pro  Glu Asn Pro Glu Glu  Pro Tyr Leu Asp Gly  Ile Asn Tyr 
    1100                 1105                 1110             


Asn Cys  Val Ala Pro Gly Lys  Arg Phe Arg Pro Met  Asp Asn Leu 
    1115                 1120                 1125             


Ser Gly  Gly Glu Lys Thr Val  Ala Ala Leu Ala Leu  Leu Phe Ala 
    1130                 1135                 1140             


Ile His  Ser Tyr Lys Pro Ala  Pro Phe Phe Val Leu  Asp Glu Ile 
    1145                 1150                 1155             


Asp Ala  Ala Leu Asp Asn Thr  Asn Ile Gly Lys Val  Ala Asn Tyr 
    1160                 1165                 1170             


Ile Lys  Glu Gln Ser Thr Cys  Asn Phe Gln Ala Ile  Val Ile Ser 
    1175                 1180                 1185             


Leu Lys  Glu Glu Phe Tyr Thr  Lys Ala Glu Ser Leu  Ile Gly Val 
    1190                 1195                 1200             


Tyr Pro  Glu Gln Gly Asp Cys  Val Ile Ser Lys Val  Leu Thr Phe 
    1205                 1210                 1215             


Asp Leu  Thr Lys Tyr Pro Asp  Ala Asn Pro Asn Pro  Asn Glu Gln 
    1220                 1225                 1230             


<210>  7
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Wherein the Serine at position 7 can be phosphorylated Serine

<400>  7

Ser Gln Glu Glu Gly Ser Ser Gln Gly Glu Glu Ser 
1               5                   10          


<210>  8
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Wherein the Serine at position 7 can be phosphorylated Serine

<400>  8

Ser Gln Glu Glu Gly Gly Ser Gln Gly Glu Glu Ser 
1               5                   10          


<210>  9
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Wherein the Serine at position 6 can be phosphorylated Serine

<400>  9

Asp Ser Val Ser Gly Ser Gln Arg Thr Ser Ser 
1               5                   10      


<210>  10
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Wherein the Serine at position 6 can be phosphorylated Serine

<400>  10

Glu Ser Val Ser Gly Ser Gln Arg Thr Ser Ser 
1               5                   10      


<210>  11
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  11

Cys Gly Ser Gly Ser Gln Glu Glu Gly Ser Ser Gln Gly Glu Asp Ser 
1               5                   10                  15      


<210>  12
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  12

Cys Gly Ser Gly Asp Ser Val Ser Gly Ser Gln Arg Ile Ser Ser 
1               5                   10                  15  


<210>  13
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  13

Cys Gly Ser Gly 
1               


<210>  14
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Wherein the Serine at position 7 can be phosphorylated Serine

<400>  14

Ser Gln Glu Glu Gly Ser Ser Gln Gly Glu Asp Ser 
1               5                   10          


