                         SEQUENCE LISTING

<110>  STC.UNM
       DURVASULA, Ravi V.
       KANG, Angray S.
       MARKIV, Anatoliy
 
<120>  FLUORESCENT FUSION POLYPEPTIDES AND METHODS OF USE

<130>  310.00670201

<150>  US 61/343,627
<151>  2010-04-30

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  1400
<212>  DNA
<213>  artificial

<220>
<223>  plasmid sequence for VH, VL, and fluorophore of B72.3RFP

<400>  1
ccatggccca ggtgcagctg cagcagagcg atgcggaact ggtgaaaccg ggcgcgagcg       60

tgaaaattag ctgcaaagcg agcggctata cctttaccga tcatgcgatt cattgggcga      120

aacagaaacc ggaacagggc ctggaatgga ttggctatat tagcccgggc aacgatgata      180

ttaaatataa cgaaaaattt aaaggcaaag cgaccctgac cgcggataaa agcagcagca      240

ccgcgtatat gcagctgaac agcctgacca gcgaagatag cgcggtgtat ttttgcaaac      300

gcagctatta tggccattgg ggccagggca ccaccctgac cgtgagcagc ggcggaggcg      360

gatccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga      420

acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga      480

ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc      540

ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact      600

tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg      660

gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga      720

agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct      780

gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaaga      840

tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg      900

ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct      960

cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc cgcacaggcg     1020

gaggcggatc cgatattcag atgacccaga gcccggcgag cctgagcgtg agcgtgggcg     1080

aaaccgtgac cattacctgc cgcgcgagcg aaaacattta tagcaacctg gcgtggtatc     1140

agcagaaaca gggcaaaagc ccgcagctgc tggtgtatgc ggcgaccaac ctggcggatg     1200

gcgtgccgag ccgctttagc ggcagcggca gcggcaccca gtatagcctg aaaattaaca     1260

gcctgcagag cgaagatttt ggcagctatt attgccagca cttttggggc accccgtata     1320

cctttggcgg cggcacccgc ctggaaatta aacgcgcgga tgcggccgca ctcgagcacc     1380

accaccacca ccaccaccac                                                 1400


<210>  2
<211>  466
<212>  PRT
<213>  artificial

<220>
<223>  amino acid sequence for VH, VL, and fluorophore of B72.3RFP


<220>
<221>  MISC_FEATURE
<222>  (1)..(116)
<223>  VH domain

<220>
<221>  MISC_FEATURE
<222>  (122)..(338)
<223>  fluorescent mRFP domain

<220>
<221>  MISC_FEATURE
<222>  (344)..(458)
<223>  VL domain

<400>  2

Met Ala Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro 
1               5                   10                  15      


Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
            20                  25                  30          


Asp His Ala Ile His Trp Ala Lys Gln Lys Pro Glu Gln Gly Leu Glu 
        35                  40                  45              


Trp Ile Gly Tyr Ile Ser Pro Gly Asn Asp Asp Ile Lys Tyr Asn Glu 
    50                  55                  60                  


Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 
65                  70                  75                  80  


Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
                85                  90                  95      


Phe Cys Lys Arg Ser Tyr Tyr Gly His Trp Gly Gln Gly Thr Thr Leu 
            100                 105                 110         


Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Asp Val Ile Lys Glu Phe 
        115                 120                 125             


Met Arg Phe Lys Val Arg Met Glu Gly Ser Val Asn Gly His Glu Phe 
    130                 135                 140                 


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
145                 150                 155                 160 


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
                165                 170                 175     


Ile Leu Ser Pro Gln Phe Gln Tyr Gly Ser Lys Ala Tyr Val Lys His 
            180                 185                 190         


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
        195                 200                 205             


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
    210                 215                 220                 


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
225                 230                 235                 240 


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
                245                 250                 255     


Thr Met Gly Trp Glu Ala Ser Thr Glu Arg Met Tyr Pro Glu Asp Gly 
            260                 265                 270         


Ala Leu Lys Gly Glu Ile Lys Met Arg Leu Lys Leu Lys Asp Gly Gly 
        275                 280                 285             


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Met Ala Lys Lys Pro Val 
    290                 295                 300                 


Gln Leu Pro Gly Ala Tyr Lys Thr Asp Ile Lys Leu Asp Ile Thr Ser 
305                 310                 315                 320 


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
                325                 330                 335     


Arg Thr Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ala 
            340                 345                 350         


Ser Leu Ser Val Ser Val Gly Glu Thr Val Thr Ile Thr Cys Arg Ala 
        355                 360                 365             


Ser Glu Asn Ile Tyr Ser Asn Leu Ala Trp Tyr Gln Gln Lys Gln Gly 
    370                 375                 380                 


Lys Ser Pro Gln Leu Leu Val Tyr Ala Ala Thr Asn Leu Ala Asp Gly 
385                 390                 395                 400 


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu 
                405                 410                 415     


Lys Ile Asn Ser Leu Gln Ser Glu Asp Phe Gly Ser Tyr Tyr Cys Gln 
            420                 425                 430         


His Phe Trp Gly Thr Pro Tyr Thr Phe Gly Gly Gly Thr Arg Leu Glu 
        435                 440                 445             


Ile Lys Arg Ala Asp Ala Ala Ala Leu Glu His His His His His His 
    450                 455                 460                 


His His 
465     


<210>  3
<211>  672
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence for mRFP1

<400>  3
ggatccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg       60

aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag      120

accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc      180

cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac      240

ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc      300

ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg      360

aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc      420

tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag      480

atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg      540

gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc      600

tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgcacaggc      660

ggaggcggat cc                                                          672


<210>  4
<211>  224
<212>  PRT
<213>  artificial

<220>
<223>  amino acid sequence for mRFP1


<220>
<221>  MISC_FEATURE
<222>  (3)..(219)
<223>  fluorescent domain

<400>  4

Gly Ser Glu Asp Val Ile Lys Glu Phe Met Arg Phe Lys Val Arg Met 
1               5                   10                  15      


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
            20                  25                  30          


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
        35                  40                  45              


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Gln 
    50                  55                  60                  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
65                  70                  75                  80  


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
                85                  90                  95      


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
            100                 105                 110         


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
        115                 120                 125             


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
    130                 135                 140                 


Thr Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
145                 150                 155                 160 


Met Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
                165                 170                 175     


Thr Thr Tyr Met Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Lys 
            180                 185                 190         


Thr Asp Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
        195                 200                 205             


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg Thr Gly Gly Gly Gly Ser 
    210                 215                 220                 


<210>  5
<211>  720
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence for mCitrine

<400>  5
ggatccgatc cgatggtgag caaaggcgaa gaactgttta ccggcgtggt gccgattctg       60

gtggaactgg atggcgatgt gaacggccat aaatttagcg tgagcggcga aggcgaaggc      120

gatgcgacct atggcaaact gaccctgaaa tttatttgca ccaccggcaa actgccggtg      180

ccgtggccga ccctggtgac cacctttggc tatggcctga tgtgctttgc gcgttatccg      240

gatcacatga aacagcatga tttttttaaa agcgcgatgc cggaaggcta tgtgcaggaa      300

cgtaccattt tttttaaaga tgatggcaac tataaaaccc gtgcggaagt gaaatttgaa      360

ggcgataccc tggtgaaccg tattgaactg aaaggcattg attttaaaga agatggcaac      420

attctgggcc ataaactgga atataactat aacagccata acgtgtatat tatggcggat      480

aaacagaaaa acggcattaa agtgaacttt aaaattcgtc ataacattga agatggcagc      540

gtgcagctgg cggatcatta tcagcagaac accccgattg gcgatggccc ggtgctgctg      600

ccggataacc attatctgag ctatcagagc gcgctgagca aagatccgaa cgaaaaacgt      660

gatcacatgg tgctgctgga atttgtgacc gcggcgggca ttaccggcgg cggcggatcc      720


<210>  6
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  amino acid sequence for mCitrine


<220>
<221>  MISC_FEATURE
<222>  (3)..(235)
<223>  fluorescent domain

<400>  6

Gly Ser Asp Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val 
1               5                   10                  15      


Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe 
            20                  25                  30          


Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr 
        35                  40                  45              


Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
    50                  55                  60                  


Leu Val Thr Thr Phe Gly Tyr Gly Leu Met Cys Phe Ala Arg Tyr Pro 
65                  70                  75                  80  


Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly 
                85                  90                  95      


Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys 
            100                 105                 110         


Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile 
        115                 120                 125             


Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His 
    130                 135                 140                 


Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp 
145                 150                 155                 160 


Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile 
                165                 170                 175     


Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
            180                 185                 190         


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr 
        195                 200                 205             


Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
    210                 215                 220                 


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Gly Gly Gly Gly Ser 
225                 230                 235                 240 


<210>  7
<211>  720
<212>  DNA
<213>  artificial

<220>
<223>  nucleotide sequence for mCerulean

<400>  7
ggatccgatc cgatggtgag caaaggcgaa gaactgttta ccggcgtggt gccgattctg       60

gtggaactgg atggcgatgt gaacggccat aaatttagcg tgagcggcga aggcgaaggc      120

gatgcgacct atggcaaact gaccctgaaa tttatttgca ccaccggcaa actgccggtg      180

ccgtggccga ccctggtgac caccctgacc tggggcgtgc agtgctttgc gcgttatccg      240

gatcacatga aacagcatga tttttttaaa agcgcgatgc cggaaggcta tgtgcaggaa      300

cgtaccattt tttttaaaga tgatggcaac tataaaaccc gtgcggaagt gaaatttgaa      360

ggcgataccc tggtgaaccg tattgaactg aaaggcattg attttaaaga agatggcaac      420

attctgggcc ataaactgga atataacgcg attagcgata acgtgtatat taccgcggat      480

aaacagaaaa acggcattaa agcgaacttt aaaattcgtc ataacattga agatggcagc      540

gtgcagctgg cggatcatta tcagcagaac accccgattg gcgatggccc ggtgctgctg      600

ccggataacc attatctgag cacccagagc gcgctgagca aagatccgaa cgaaaaacgt      660

gatcacatgg tgctgctgga atttgtgacc gcggcgggca ttaccggcgg cggcggatcc      720


<210>  8
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  amino acid sequence for mCerulean


<220>
<221>  MISC_FEATURE
<222>  (3)..(235)
<223>  fluorescent domain

<400>  8

Gly Ser Asp Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val 
1               5                   10                  15      


Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe 
            20                  25                  30          


Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr 
        35                  40                  45              


Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
    50                  55                  60                  


Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe Ala Arg Tyr Pro 
65                  70                  75                  80  


Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly 
                85                  90                  95      


Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys 
            100                 105                 110         


Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile 
        115                 120                 125             


Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His 
    130                 135                 140                 


Lys Leu Glu Tyr Asn Ala Ile Ser Asp Asn Val Tyr Ile Thr Ala Asp 
145                 150                 155                 160 


Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile 
                165                 170                 175     


Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
            180                 185                 190         


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr 
        195                 200                 205             


Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
    210                 215                 220                 


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Gly Gly Gly Gly Ser 
225                 230                 235                 240 


<210>  9
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  9
gtcccttctc gcttctctgg gtccagatct gggacggatt tcac                        44


<210>  10
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  10
gtgaaatccg tcccagatct ggacccagag aagcgagaag ggac                        44


<210>  11
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  11
cagcggcgga ggcggatccg atatccagat gacccagtc                              39


<210>  12
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  12
tcgagtgcgg ccgcatccgc gcgtttgatc tccaccttgg tac                         43


<210>  13
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  13
tcggatccgc ctccgcccga ggagacggtg accagggttc                             40


<210>  14
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  14
taggccatgg ccgaggttca gctggtggag                                        30


<210>  15
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  15
cagtggatcc gaggacgtca tcaaggagtt c                                      31


<210>  16
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  16
cagtggatcc gcctccgcct gtgcggccct cggcgcgctc gtac                        44


<210>  17
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  17
cagtggatcc gccgccgccg gtaatgcccg ccgcggtcac                             40


<210>  18
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  18
cagtggatcc gaggacgtca tcaaggagtt c                                      31


<210>  19
<211>  44
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  19
cagtggatcc gcctccgcct gtgcggccct cggcgcgctc gtac                        44


<210>  20
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  synthetic oligonucleotide primer

<400>  20
gcagctaata cgactcacta tagg                                              24


