                         SEQUENCE LISTING

<110>  The Chinese University of Hong Kong
       Yu, Jun
       Sung, Joseph Jao Yiu
       Li, Xiaoxing
       
 
<120>  MARKER FOR GASTRIC CANCER AND METHOD FOR DETECTING GASTRIC CANCER

<130>  11C50189

<150>  US12/771,672
<151>  2010-04-30

<160>  24    

<170>  PatentIn version 3.3

<210>  1
<211>  20
<212>  DNA
<213>  forward primer

<400>  1
gtccattcca tcaagtcctg                                                 20


<210>  2
<211>  20
<212>  DNA
<213>  reverse primer

<400>  2
ttgctgacac aaccatggct                                                 20


<210>  3
<211>  30
<212>  DNA
<213>  forward primer

<400>  3
atataagctt gtccattcca tcaagtcctg                                      30


<210>  4
<211>  29
<212>  DNA
<213>  reverse primer

<400>  4
atatctcgag agggtcagtg acggtcata                                       29


<210>  5
<211>  25
<212>  DNA
<213>  forward primer

<400>  5
gtttttttaa aagtatttgt ttggt                                           25


<210>  6
<211>  21
<212>  DNA
<213>  reverse primer

<400>  6
gcaccttcta ttaaaacata c                                               21


<210>  7
<211>  23
<212>  DNA
<213>  PAX5mF2 forward primer

<400>  7
aaataaaaat tcggtttgcg ttc                                             23


<210>  8
<211>  22
<212>  DNA
<213>  PAX5mR2 reverse primer

<400>  8
aaacatacgc ttaaaaatcg cg                                              22


<210>  9
<211>  26
<212>  DNA
<213>  PAX5uF2 forward primer

<400>  9
taaaaataaa aatttggttt gtgttt                                          26


<210>  10
<211>  25
<212>  DNA
<213>  PAX5uR2 reverse primer

<400>  10
ttaaaacata cacttaaaaa tcaca                                           25


<210>  11
<211>  105
<212>  DNA
<213>  Sequence of promoter region of human PAX5 gene (-248 to -144 from the transcription start site)

<400>  11
aaacaaaaac ccggcctgcg ctcgtctaag cagcggggtt tgcacatgga gatgtcacag     60

gccccgcgca cagcgcagag ggccgcgacc cccaagcgca tgtct                    105


<210>  12
<211>  1176
<212>  DNA
<213>  PAX5 protein coding cDNA sequence

<400>  12
atggatttag agaaaaatta tccgactcct cggaccagca ggacaggaca tggaggagtg     60

aatcagcttg ggggggtttt tgtgaatgga cggccactcc cggatgtagt ccgccagagg    120

atagtggaac ttgctcatca aggtgtcagg ccctgcgaca tctccaggca gcttcgggtc    180

agccatggtt gtgtcagcaa aattcttggc aggtattatg agacaggaag catcaagcct    240

ggggtaattg gaggatccaa accaaaggtc gccacaccca aagtggtgga aaaaatcgct    300

gaatataaac gccaaaatcc caccatgttt gcctgggaga tcagggaccg gctgctggca    360

gagcgggtgt gtgacaatga caccgtgcct agcgtcagtt ccatcaacag gatcatccgg    420

acaaaagtac agcagccacc caaccaacca gtcccagctt ccagtcacag catagtgtcc    480

actggctccg tgacgcaggt gtcctcggtg agcacggatt cggccggctc gtcgtactcc    540

atcagcggca tcctgggcat cacgtccccc agcgccgaca ccaacaagcg caagagagac    600

gaaggtattc aggagtctcc ggtgccgaac ggccactcgc ttccgggcag agacttcctc    660

cggaagcaga tgcggggaga cttgttcaca cagcagcagc tggaggtgct ggaccgcgtg    720

tttgagaggc agcactactc agacatcttc accaccacag agcccatcaa gcccgagcag    780

accacagagt attcagccat ggcctcgctg gctggtgggc tggacgacat gaaggccaat    840

ctggccagcc ccacccctgc tgacatcggg agcagtgtgc caggcccgca gtcctacccc    900

attgtgacag gccgtgactt ggcgagcacg accctccccg ggtaccctcc acacgtcccc    960

cccgctggac agggcagcta ctcagcaccg acgctgacag ggatggtgcc tgggagtgag   1020

ttttccggga gtccctacag ccaccctcag tattcctcgt acaacgactc ctggaggttc   1080

cccaacccgg ggctgcttgg ctccccctac tattatagcg ctgccgcccg aggagccgcc   1140

ccacctgcag ccgccactgc ctatgaccgt cactga                             1176


<210>  13
<211>  3650
<212>  DNA
<213>  PAX5 full length cDNA sequence

<400>  13
agcactgctg ctctcccggc ttcccgctct actccggccg ggccgggtcc gccacgtctg     60

gcgcgctgag caggcccggc cgcgcagcgc ctaccctttc ctcgctccgg gccggcagtg    120

tggggcggcg cgctgggggc gcggcgtgtc tggggacatc ttgtgatgtt ggcgagaaca    180

ggacatgatc tcacatggcg agaagctctt tagttcctta atcatttcac ggtgccttcg    240

gacgcttttt ttccacctaa aacgtttagt ttcagctcag tgatcagcta ccccagctcg    300

gcgggggagc ggaaggcttg aattattccg acctgtgagc ggcccctggc accaaaaaaa    360

aaaaaaaaaa aaaaaaaaaa agaaaaaaaa aggcacaaaa aagtggaaac ttttccctgt    420

ccattccatc aagtcctgaa aaatcaaaat ggatttagag aaaaattatc cgactcctcg    480

gaccagcagg acaggacatg gaggagtgaa tcagcttggg ggggtttttg tgaatggacg    540

gccactcccg gatgtagtcc gccagaggat agtggaactt gctcatcaag gtgtcaggcc    600

ctgcgacatc tccaggcagc ttcgggtcag ccatggttgt gtcagcaaaa ttcttggcag    660

gtattatgag acaggaagca tcaagcctgg ggtaattgga ggatccaaac caaaggtcgc    720

cacacccaaa gtggtggaaa aaatcgctga atataaacgc caaaatccca ccatgtttgc    780

ctgggagatc agggaccggc tgctggcaga gcgggtgtgt gacaatgaca ccgtgcctag    840

cgtcagttcc atcaacagga tcatccggac aaaagtacag cagccaccca accaaccagt    900

cccagcttcc agtcacagca tagtgtccac tggctccgtg acgcaggtgt cctcggtgag    960

cacggattcg gccggctcgt cgtactccat cagcggcatc ctgggcatca cgtcccccag   1020

cgccgacacc aacaagcgca agagagacga aggtattcag gagtctccgg tgccgaacgg   1080

ccactcgctt ccgggcagag acttcctccg gaagcagatg cggggagact tgttcacaca   1140

gcagcagctg gaggtgctgg accgcgtgtt tgagaggcag cactactcag acatcttcac   1200

caccacagag cccatcaagc ccgagcagac cacagagtat tcagccatgg cctcgctggc   1260

tggtgggctg gacgacatga aggccaatct ggccagcccc acccctgctg acatcgggag   1320

cagtgtgcca ggcccgcagt cctaccccat tgtgacaggc cgtgacttgg cgagcacgac   1380

cctccccggg taccctccac acgtcccccc cgctggacag ggcagctact cagcaccgac   1440

gctgacaggg atggtgcctg ggagtgagtt ttccgggagt ccctacagcc accctcagta   1500

ttcctcgtac aacgactcct ggaggttccc caacccgggg ctgcttggct ccccctacta   1560

ttatagcgct gccgcccgag gagccgcccc acctgcagcc gccactgcct atgaccgtca   1620

ctgacccttg gagccaggcg ggcaccaaac actgatggca cctattgagg gtgacagcca   1680

cccagccctc ctgaagatag ccagagagcc catgagaccg tcccccagca tcccccactt   1740

gcctgaagct cccctcttcc tctcttcctc cagggactct ggggcccttt ggtggggccg   1800

ttggacttct ggatgcttgt ctatttctaa aagccaatct atgagcttct cccgatggcc   1860

actgggtctc tgcaaaccaa tagactgtcc tgcaaataac cgcagcccca gcccagcctg   1920

cctgtcctcc agctgtctga ctatccatcc atcataacca ccccagcctg ggaaggagag   1980

cttgcttttg ttgcttcagc agcacccatg taaatacctt cttgcttttc tgtgggcctg   2040

aaggtccgac tgagaagact gctccaccca tgatgcatct cgcactcttg gtgcatcacc   2100

ggacatctta gacctatggc agagcatcct ctctgccctg ggtgaccctg gcaggtgcgc   2160

tcagagctgt cctcaagatg gaggatgctg cccttgggcc ccagcctcct gctcatccct   2220

ccttctttag tatctttacg aggagtctca ctgggctggt tgtgctgcag gctccccctg   2280

aggcccctct ccaagaggag cacactttgg ggagatgtcc tggtttcctg cctccatttc   2340

tctgggaccg atgcagtatc agcagctctt ttccagatca aagaactcaa agaaaactgt   2400

ctgggagatt cctcagctac ttttccgaag cagaatgtca tccgaggtat tgattacatt   2460

gtggactttg aatgtgaggg ctggatggga cgcaggagat catctgatcc cagccaagga   2520

ggggcctgag gctctcccta ctccctcagc ccctggaacg gtgttttctg aggcatgccc   2580

aggttcaggt cacttcggac acctgccatg gacacttcac ccaccctcca ggaccccagc   2640

aagtggattc tgggcaagcc tgttccggtg atgtagacaa taattaacac agaggacttt   2700

cccccacacc cagatcacaa acagcctaca gccagaactt ctgagcatcc tctcggggca   2760

gaccctcccc gtcctcgtgg agcttagcag gcagctgggc atggaggtgc tggggctggg   2820

gcagatgcct aatttcgcac aatgcatgcc cacctgttga tctaaggggc cgcgatggtc   2880

agggccacgg ccaagggcca cgggaacttg gagagggagc ttggagaact cactgtgggc   2940

tagggtggtc agaggaagcc agcagggaag atctggggga cagaggaagg cctcctgagg   3000

gaggggcagg agagcagtga ggagctgctg tgtgacctgg gagtgatttt gacatggggg   3060

tgccaggtgc catcatctct ttacctgggg ccttaattcc ttgcatagtc tctcttgtca   3120

agtcagaaca gccaggtaga gcccttgtcc aaacctgggc tgaatgacag tgatgagagg   3180

gggcttggcc ttcttaggtg acaatgtccc ccatatctgt atgtcaccag gatggcagag   3240

agccagggca gagagagact ggacttggga tcagcaggcc aggcaggtct tgtcctggtc   3300

ctggccacat gtctttgctg tgggacctca gacaaaaccc tgcacctctt tgagccttgg   3360

ctgccttggt gcagcagggt catctgtagg gccaccccac agctctttcc ttcccctcct   3420

ctctccaggg agccggggct gtgagaggat catctggggc aggccctcca cttccaagca   3480

agcagatggg ggtgggcacc tgaggcccaa taatatttgg accaagtggg aaacaagaac   3540

actcggaggg gcgggaatca gaagagcctg gaaaaagacc tagcccaact tcccttgtgg   3600

gaaactgagg cccagcttgg ggaaggccag gaccatgcag ggagaaaaag              3650


<210>  14
<211>  230
<212>  DNA
<213>  Partial coding sequence used to detect human PAX5 mRNA 

<400>  14
gtccattcca tcaagtcctg aaaaatcaaa atggatttag agaaaaatta tccgactcct     60

cggaccagca ggacaggaca tggaggagtg aatcagcttg ggggggtttt tgtgaatgga    120

cggccactcc cggatgtagt ccgccagagg atagtggaac ttgctcatca aggtgtcagg    180

ccctgcgaca tctccaggca gcttcgggtc agccatggtt gtgtcagcaa               230


<210>  15
<211>  21
<212>  DNA
<213>  PAX5mF1 forward primer

<400>  15
tgaatcggag taaatcggaa c                                               21


<210>  16
<211>  19
<212>  DNA
<213>  PAX5mR1 reverse primer

<400>  16
cccgctactt aaacgaacg                                                  19


<210>  17
<211>  23
<212>  DNA
<213>  PAX5uF1 forward primer

<400>  17
ggtgaattgg agtaaattgg aat                                             23


<210>  18
<211>  20
<212>  DNA
<213>  PAX5uR1 reverse primer

<400>  18
ccccactact taaacaaaca                                                 20


<210>  19
<211>  23
<212>  DNA
<213>  PAX5mF3 Forward primer

<400>  19
gcgtatgttt taatagaagg tgc                                             23


<210>  20
<211>  20
<212>  DNA
<213>  PAX5mR3 reverse primer

<400>  20
acttcaacct acgccgaacg                                                 20


<210>  21
<211>  24
<212>  DNA
<213>  PAX5uF3 Forward primer

<400>  21
agtgtatgtt ttaatagaag gtgt                                            24


<210>  22
<211>  21
<212>  DNA
<213>  PAX5uR3 reverse primer

<400>  22
aacttcaacc tacaccaaac a                                               21


<210>  23
<211>  391
<212>  PRT
<213>  PAX5_HUMAN Paired box protein Pax-5

<400>  23

Met Asp Leu Glu Lys Asn Tyr Pro Thr Pro Arg Thr Ser Arg Thr Gly 
1               5                   10                  15      


His Gly Gly Val Asn Gln Leu Gly Gly Val Phe Val Asn Gly Arg Pro 
            20                  25                  30          


Leu Pro Asp Val Val Arg Gln Arg Ile Val Glu Leu Ala His Gln Gly 
        35                  40                  45              


Val Arg Pro Cys Asp Ile Ser Arg Gln Leu Arg Val Ser His Gly Cys 
    50                  55                  60                  


Val Ser Lys Ile Leu Gly Arg Tyr Tyr Glu Thr Gly Ser Ile Lys Pro 
65                  70                  75                  80  


Gly Val Ile Gly Gly Ser Lys Pro Lys Val Ala Thr Pro Lys Val Val 
                85                  90                  95      


Glu Lys Ile Ala Glu Tyr Lys Arg Gln Asn Pro Thr Met Phe Ala Trp 
            100                 105                 110         


Glu Ile Arg Asp Arg Leu Leu Ala Glu Arg Val Cys Asp Asn Asp Thr 
        115                 120                 125             


Val Pro Ser Val Ser Ser Ile Asn Arg Ile Ile Arg Thr Lys Val Gln 
    130                 135                 140                 


Gln Pro Pro Asn Gln Pro Val Pro Ala Ser Ser His Ser Ile Val Ser 
145                 150                 155                 160 


Thr Gly Ser Val Thr Gln Val Ser Ser Val Ser Thr Asp Ser Ala Gly 
                165                 170                 175     


Ser Ser Tyr Ser Ile Ser Gly Ile Leu Gly Ile Thr Ser Pro Ser Ala 
            180                 185                 190         


Asp Thr Asn Lys Arg Lys Arg Asp Glu Gly Ile Gln Glu Ser Pro Val 
        195                 200                 205             


Pro Asn Gly His Ser Leu Pro Gly Arg Asp Phe Leu Arg Lys Gln Met 
    210                 215                 220                 


Arg Gly Asp Leu Phe Thr Gln Gln Gln Leu Glu Val Leu Asp Arg Val 
225                 230                 235                 240 


Phe Glu Arg Gln His Tyr Ser Asp Ile Phe Thr Thr Thr Glu Pro Ile 
                245                 250                 255     


Lys Pro Glu Gln Thr Thr Glu Tyr Ser Ala Met Ala Ser Leu Ala Gly 
            260                 265                 270         


Gly Leu Asp Asp Met Lys Ala Asn Leu Ala Ser Pro Thr Pro Ala Asp 
        275                 280                 285             


Ile Gly Ser Ser Val Pro Gly Pro Gln Ser Tyr Pro Ile Val Thr Gly 
    290                 295                 300                 


Arg Asp Leu Ala Ser Thr Thr Leu Pro Gly Tyr Pro Pro His Val Pro 
305                 310                 315                 320 


Pro Ala Gly Gln Gly Ser Tyr Ser Ala Pro Thr Leu Thr Gly Met Val 
                325                 330                 335     


Pro Gly Ser Glu Phe Ser Gly Ser Pro Tyr Ser His Pro Gln Tyr Ser 
            340                 345                 350         


Ser Tyr Asn Asp Ser Trp Arg Phe Pro Asn Pro Gly Leu Leu Gly Ser 
        355                 360                 365             


Pro Tyr Tyr Tyr Ser Ala Ala Ala Arg Gly Ala Ala Pro Pro Ala Ala 
    370                 375                 380                 


Ala Thr Ala Tyr Asp Arg His 
385                 390     


<210>  24
<211>  307
<212>  DNA
<213>  Sequence of promoter region of PAX5 gene

<400>  24
tgaatcggag taaaccggaa cgtcgcctcg gtgcctggca acactgccag cctttgcaac     60

ccctttcaaa agcacctgct tggccgagca gttaatttct tcaaaaacaa aaacccggcc    120

tgcgctcgtc taagcagcgg ggtttgcaca tggagatgtc acaggccccg cgcacagcgc    180

agagggccgc gacccccaag cgcatgtctt aatagaaggt gcggctggaa gacccgggct    240

cccgggctcc gcttcggtct gccccttccc gtaggtgcgc tggctagcgc ccggcgcagg    300

ctgaagc                                                              307


