                         SEQUENCE LISTING

<110>  Senesco Technologies, Inc.
       LOUCAS, Holly
       WANG, Tzann-Wei
       THOMPSON, John
 
<120>  Transgenic Algae with Enhanced Oil Expression

<130>  061945-5033-WO

<150>  US 61/326,979
<151>  2010-04-22

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  1832
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence of the pPGK:PdF5A3cDNA-tNos construct


<220>
<221>  promoter
<222>  (1)..(737)
<223>  PGK1 promoter

<220>
<221>  misc_feature
<222>  (738)..(1561)
<223>  Poplar eIF5A3 full length cDNA (including 5'- and 3'-UTR) 
       sequence

<220>
<221>  terminator
<222>  (1562)..(1832)
<223>  Nos terminator

<400>  1
aggcatttgc aagaattact cgtgagtaag gaaagagtga ggaactatcg catacctgca       60

tttaaagatg ccgatttggg cgcgaatcct ttattttggc ttcaccctca tactattatc      120

agggccagaa aaaggaagtg tttccctcct tcttgaattg atgttaccct cataaagcac      180

gtggcctctt atcgagaaag aaattaccgt cgctcgtgat ttgtttgcaa aaagaacaaa      240

actgaaaaaa cccagacacg ctcgacttcc tgtcttccta ttgattgcag cttccaattt      300

cgtcacacaa caaggtccta gcgacggctc acaggttttg taacaagcaa tcgaaggttc      360

tggaatggcg ggaaagggtt tagtaccaca tgctatgatg cccactgtga tctccagagc      420

aaagttcgtt cgatcgtact gttactctct ctctttcaaa cagaattgtc cgaatcgtgt      480

gacaacaaca gcctgttctc acacactctt ttcttctaac caagggggtg gtttagttta      540

gtagaacctc gtgaaactta catttacata tatataaact tgcataaatt ggtcaatgca      600

agaaatacat atttggtctt ttctaattcg tagtttttca agttcttaga tgctttcttt      660

ttctcttttt tacagatcat caaggaagta attatctact ttttacaaca aatataaaac      720

aggatccaaa gaattcgggt attttgtttg ttttgtgatt gcgactctct tatcaatcgc      780

ggccatgtct gacgaggagc agcacttcga gtcaaaagct gatgcgggag cttcgaaaac      840

ttaccctcaa caagctggta ccattcgcaa gagcggttac attgtcatca agaatcgccc      900

ttgcaaggtt gtggaggttt ctacctctaa aactggcaag cacggccatg ccaaatgtca      960

ctttgttgca attgatatct tcaatggaaa aaagcttgaa gatattgttc cttcttccca     1020

caactgtgat gttccccatg tcacccgtac tgactatcag ctgattgata tctcagagga     1080

tggatttgtg agcttgctga ctgagaatgg caataccaag gatgacctga ggctcccaac     1140

tgatgagagt ctcctctctc agatcaagga tggatttggc gagggtaaag atcttgttgt     1200

gactgtgatg tcctccatgg gagaggagca gatctgcgcc ctcaaggacg ttggcccgaa     1260

gtaaattgta ccctgcttca tgcaggcatg cccaaggttg acggctgata gttgtagctt     1320

agcaagtggt tctatgtggg tttgagaatt ggtcccgttt caggaacatt ccgtactaga     1380

tgcggccttc ccttatatga agacttgcca ttatttgagt ggcgttttat ccgtgatgat     1440

atttttgtgg ttcttggtgt ggggaagttt attctgccag taagactgca tacacttctt     1500

ggcacctgtg gtggtttcaa acaattacag tttttatttc cttaaaaaaa aaaaaaaaaa     1560

actcgagagt acttctagag atcgttcaaa catttggcaa taaagtttct taagattgaa     1620

tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt     1680

aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc     1740

gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt     1800

atcgcgcgcg gtgtcatcta tgttactaga tc                                   1832


<210>  2
<211>  2126
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence of the pPGK:TDHS-tTEF1 construct


<220>
<221>  promoter
<222>  (1)..(733)
<223>  PGK1 promoter

<220>
<221>  misc_feature
<222>  (734)..(1879)
<223>  Poplar DHS coding sequence

<220>
<221>  terminator
<222>  (1880)..(2126)
<223>  TEF1 terminator

<400>  2
aagcttaggc atttgcaaga attactcgtg agtaaggaaa gagtgaggaa ctatcgcata       60

cctgcattta aagatgccga tttgggcgcg aatcctttat tttggcttca ccctcatact      120

attatcaggg ccagaaaaag gaagtgtttc cctccttctt gaattgatgt taccctcata      180

aagcacgtgg cctcttatcg agaaagaaat taccgtcgct cgtgatttgt ttgcaaaaag      240

aacaaaactg aaaaaaccca gacacgctcg acttcctgtc ttcctattga ttgcagcttc      300

caatttcgtc acacaacaag gtcctagcga cggctcacag gttttgtaac aagcaatcga      360

aggttctgga atggcgggaa agggtttagt accacatgct atgatgccca ctgtgatctc      420

cagagcaaag ttcgttcgat cgtactgtta ctctctctct ttcaaacaga attgtccgaa      480

tcgtgtgaca acaacagcct gttctcacac actcttttct tctaaccaag ggggtggttt      540

agtttagtag aacctcgtga aacttacatt tacatatata taaacttgca taaattggtc      600

aatgcaagaa atacatattt ggtcttttct aattcgtagt ttttcaagtt cttagatgct      660

ttctttttct cttttttaca gatcatcaag gaagtaatta tctacttttt acaacaaata      720

taaaacactc gagatgggag aagctctgaa gtacagtatc atggactcag taagatcggt      780

agttttcaaa gaatccgaaa atctagaagg ttcttgcact aaaatcgagg gctacgactt      840

caataaaggc gttaactatg ctgagctgat caagtccatg gtttccactg gtttccaagc      900

atctaatctt ggtgacgcca ttgcaattgt taatcaaatg ctagattgga ggctttcaca      960

tgagctgccc acggaggatt gcagtgaaga agaaagagat gttgcataca gagagtcggt     1020

aacctgcaaa atcttcttgg ggttcacttc aaaccttgtt tcttctggtg ttagagacac     1080

tgtccgctac cttgttcagc accggatggt tgatgttgtg gttactacag ctggtggtat     1140

tgaagaggat ctcataaagt gcctcgcacc aacctacaag ggggacttct ctttacctgg     1200

agcttctcta cgatcgaaag gattgaaccg tattggtaac ttattggttc ctaatgacaa     1260

ctactgcaaa tttgagaatt ggatcatccc agtttttgac caaatgtatg aggagcagat     1320

taatgagaag gttctatgga caccatctaa agtcattgct cgtctgggta aagaaattaa     1380

tgatgaaacc tcatacttgt attgggctta caagaaccgg attcctgtct tctgtcctgg     1440

cttgacggat ggatcacttg gtgacatgct atacttccat tctttcaaaa agggtgatcc     1500

agataatcca gatcttaatc ctggtctagt catagacatt gtaggagata ttagggccat     1560

gaatggtgaa gctgtccatg ctggtttgag gaagacagga atgattatac tgggtggagg     1620

gctgcctaag caccatgttt gcaatgccaa tatgatgcgc aatggtgcag attttgccgt     1680

cttcattaac accgcacaag agtttgatgg tagtgactct ggtgcccgtc ctgatgaagc     1740

tgtatcatgg ggaaagatac gtggtggtgc caagactgtg aaggtgcatt gtgatgcaac     1800

cattgcattt cccatattag tagctgagac atttgcagct aagagtaagg aattctccca     1860

gataaggtgc caagtttgag gatcctcagt actgacaata aaaagattct tgttttcaag     1920

aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc     1980

gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag     2040

aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga     2100

tactaacgcc gccatccagt atcgat                                          2126


<210>  3
<211>  824
<212>  DNA
<213>  Populus deltoides

<400>  3
ggtattttgt ttgttttgtg attgcgactc tcttatcaat cgcggccatg tctgacgagg       60

agcagcactt cgagtcaaaa gctgatgcgg gagcttcgaa aacttaccct caacaagctg      120

gtaccattcg caagagcggt tacattgtca tcaagaatcg cccttgcaag gttgtggagg      180

tttctacctc taaaactggc aagcacggcc atgccaaatg tcactttgtt gcaattgata      240

tcttcaatgg aaaaaagctt gaagatattg ttccttcttc ccacaactgt gatgttcccc      300

atgtcacccg tactgactat cagctgattg atatctcaga ggatggattt gtgagcttgc      360

tgactgagaa tggcaatacc aaggatgacc tgaggctccc aactgatgag agtctcctct      420

ctcagatcaa ggatggattt ggcgagggta aagatcttgt tgtgactgtg atgtcctcca      480

tgggagagga gcagatctgc gccctcaagg acgttggccc gaagtaaatt gtaccctgct      540

tcatgcaggc atgcccaagg ttgacggctg atagttgtag cttagcaagt ggttctatgt      600

gggtttgaga attggtcccg tttcaggaac attccgtact agatgcggcc ttcccttata      660

tgaagacttg ccattatttg agtggcgttt tatccgtgat gatatttttg tggttcttgg      720

tgtggggaag tttattctgc cagtaagact gcatacactt cttggcacct gtggtggttt      780

caaacaatta cagtttttat ttccttaaaa aaaaaaaaaa aaaa                       824


<210>  4
<211>  159
<212>  PRT
<213>  Populus deltoides

<400>  4

Met Ser Asp Glu Glu Gln His Phe Glu Ser Lys Ala Asp Ala Gly Ala 
1               5                   10                  15      


Ser Lys Thr Tyr Pro Gln Gln Ala Gly Thr Ile Arg Lys Ser Gly Tyr 
            20                  25                  30          


Ile Val Ile Lys Asn Arg Pro Cys Lys Val Val Glu Val Ser Thr Ser 
        35                  40                  45              


Lys Thr Gly Lys His Gly His Ala Lys Cys His Phe Val Ala Ile Asp 
    50                  55                  60                  


Ile Phe Asn Gly Lys Lys Leu Glu Asp Ile Val Pro Ser Ser His Asn 
65                  70                  75                  80  


Cys Asp Val Pro His Val Thr Arg Thr Asp Tyr Gln Leu Ile Asp Ile 
                85                  90                  95      


Ser Glu Asp Gly Phe Val Ser Leu Leu Thr Glu Asn Gly Asn Thr Lys 
            100                 105                 110         


Asp Asp Leu Arg Leu Pro Thr Asp Glu Ser Leu Leu Ser Gln Ile Lys 
        115                 120                 125             


Asp Gly Phe Gly Glu Gly Lys Asp Leu Val Val Thr Val Met Ser Ser 
    130                 135                 140                 


Met Gly Glu Glu Gln Ile Cys Ala Leu Lys Asp Val Gly Pro Lys 
145                 150                 155                 


<210>  5
<211>  1146
<212>  DNA
<213>  Solanum lycopersicum

<400>  5
atgggagaag ctctgaagta cagtatcatg gactcagtaa gatcggtagt tttcaaagaa       60

tccgaaaatc tagaaggttc ttgcactaaa atcgagggct acgacttcaa taaaggcgtt      120

aactatgctg agctgatcaa gtccatggtt tccactggtt tccaagcatc taatcttggt      180

gacgccattg caattgttaa tcaaatgcta gattggaggc tttcacatga gctgcccacg      240

gaggattgca gtgaagaaga aagagatgtt gcatacagag agtcggtaac ctgcaaaatc      300

ttcttggggt tcacttcaaa ccttgtttct tctggtgtta gagacactgt ccgctacctt      360

gttcagcacc ggatggttga tgttgtggtt actacagctg gtggtattga agaggatctc      420

ataaagtgcc tcgcaccaac ctacaagggg gacttctctt tacctggagc ttctctacga      480

tcgaaaggat tgaaccgtat tggtaactta ttggttccta atgacaacta ctgcaaattt      540

gagaattgga tcatcccagt ttttgaccaa atgtatgagg agcagattaa tgagaaggtt      600

ctatggacac catctaaagt cattgctcgt ctgggtaaag aaattaatga tgaaacctca      660

tacttgtatt gggcttacaa gaaccggatt cctgtcttct gtcctggctt gacggatgga      720

tcacttggtg acatgctata cttccattct ttcaaaaagg gtgatccaga taatccagat      780

cttaatcctg gtctagtcat agacattgta ggagatatta gggccatgaa tggtgaagct      840

gtccatgctg gtttgaggaa gacaggaatg attatactgg gtggagggct gcctaagcac      900

catgtttgca atgccaatat gatgcgcaat ggtgcagatt ttgccgtctt cattaacacc      960

gcacaagagt ttgatggtag tgactctggt gcccgtcctg atgaagctgt atcatgggga     1020

aagatacgtg gtggtgccaa gactgtgaag gtgcattgtg atgcaaccat tgcatttccc     1080

atattagtag ctgagacatt tgcagctaag agtaaggaat tctcccagat aaggtgccaa     1140

gtttga                                                                1146


<210>  6
<211>  381
<212>  PRT
<213>  Solanum lycopersicum

<400>  6

Met Gly Glu Ala Leu Lys Tyr Ser Ile Met Asp Ser Val Arg Ser Val 
1               5                   10                  15      


Val Phe Lys Glu Ser Glu Asn Leu Glu Gly Ser Cys Thr Lys Ile Glu 
            20                  25                  30          


Gly Tyr Asp Phe Asn Lys Gly Val Asn Tyr Ala Glu Leu Ile Lys Ser 
        35                  40                  45              


Met Val Ser Thr Gly Phe Gln Ala Ser Asn Leu Gly Asp Ala Ile Ala 
    50                  55                  60                  


Ile Val Asn Gln Met Leu Asp Trp Arg Leu Ser His Glu Leu Pro Thr 
65                  70                  75                  80  


Glu Asp Cys Ser Glu Glu Glu Arg Asp Val Ala Tyr Arg Glu Ser Val 
                85                  90                  95      


Thr Cys Lys Ile Phe Leu Gly Phe Thr Ser Asn Leu Val Ser Ser Gly 
            100                 105                 110         


Val Arg Asp Thr Val Arg Tyr Leu Val Gln His Arg Met Val Asp Val 
        115                 120                 125             


Val Val Thr Thr Ala Gly Gly Ile Glu Glu Asp Leu Ile Lys Cys Leu 
    130                 135                 140                 


Ala Pro Thr Tyr Lys Gly Asp Phe Ser Leu Pro Gly Ala Ser Leu Arg 
145                 150                 155                 160 


Ser Lys Gly Leu Asn Arg Ile Gly Asn Leu Leu Val Pro Asn Asp Asn 
                165                 170                 175     


Tyr Cys Lys Phe Glu Asn Trp Ile Ile Pro Val Phe Asp Gln Met Tyr 
            180                 185                 190         


Glu Glu Gln Ile Asn Glu Lys Val Leu Trp Thr Pro Ser Lys Val Ile 
        195                 200                 205             


Ala Arg Leu Gly Lys Glu Ile Asn Asp Glu Thr Ser Tyr Leu Tyr Trp 
    210                 215                 220                 


Ala Tyr Lys Asn Arg Ile Pro Val Phe Cys Pro Gly Leu Thr Asp Gly 
225                 230                 235                 240 


Ser Leu Gly Asp Met Leu Tyr Phe His Ser Phe Lys Lys Gly Asp Pro 
                245                 250                 255     


Asp Asn Pro Asp Leu Asn Pro Gly Leu Val Ile Asp Ile Val Gly Asp 
            260                 265                 270         


Ile Arg Ala Met Asn Gly Glu Ala Val His Ala Gly Leu Arg Lys Thr 
        275                 280                 285             


Gly Met Ile Ile Leu Gly Gly Gly Leu Pro Lys His His Val Cys Asn 
    290                 295                 300                 


Ala Asn Met Met Arg Asn Gly Ala Asp Phe Ala Val Phe Ile Asn Thr 
305                 310                 315                 320 


Ala Gln Glu Phe Asp Gly Ser Asp Ser Gly Ala Arg Pro Asp Glu Ala 
                325                 330                 335     


Val Ser Trp Gly Lys Ile Arg Gly Gly Ala Lys Thr Val Lys Val His 
            340                 345                 350         


Cys Asp Ala Thr Ile Ala Phe Pro Ile Leu Val Ala Glu Thr Phe Ala 
        355                 360                 365             


Ala Lys Ser Lys Glu Phe Ser Gln Ile Arg Cys Gln Val 
    370                 375                 380     


<210>  7
<211>  945
<212>  DNA
<213>  Arabidopsis thaliana

<400>  7
atggaatcta atggatcagt ttcatcgatg gttaacttgg agaagtttct ctgtgagcga       60

ttggtggacc agtcacaacc aatctcggag cgatttagag ctctcttctc tcttcgcaac      120

ttgaaaggcc caggacctcg caacgctcta atccttgcat ccagagactc atctaatttg      180

ttagcacatg aagctgcatt tgcattgggt cagatgcaag atgctgaagc cattcctgct      240

cttgagtcgg ttcttaatga tatgtctttg catccaatag tacgacatga ggcagcagaa      300

gcccttggag ctattggttt ggcgggtaat gttaacattt taaagaaaag cttgagctcg      360

gatccagctc aggaggttcg ggaaacatgt gaattagctc tcaaaaggat tgaagacatg      420

agtaacgttg atgccgagaa ccagtcatca acgacagaga aatcaccttt catgtctgtt      480

gacccagcag gcccagctgc gtctttctct tctgttcacc aactcaggca agttctcctg      540

gatgaaacaa aaggcatgta tgagagatat gctgcactgt tcgctctaag gaatcatggt      600

ggagaggaag ctgtttctgc catagttgat tctttgagtg ctagtagtgc ccttctacgt      660

cacgaggttg cttatgtctt gggtcagttg caaagtaaaa ctgctttagc tactctaagc      720

aaagtactta gagatgtgaa tgagcaccca atggttagac atgaggctgc agaagcgctt      780

ggttctattg ctgatgaaca gagcattgct ttgctagaag aattctcaaa ggaccctgag      840

ccaattgttg cacaaagctg tgaagtagca ttgagtatgt tggaatttga aaattccggg      900

aaatcgtttg agtttttttt cacgcaagac ccgcttgttc actaa                      945


<210>  8
<211>  314
<212>  PRT
<213>  Arabidopsis thaliana

<400>  8

Met Glu Ser Asn Gly Ser Val Ser Ser Met Val Asn Leu Glu Lys Phe 
1               5                   10                  15      


Leu Cys Glu Arg Leu Val Asp Gln Ser Gln Pro Ile Ser Glu Arg Phe 
            20                  25                  30          


Arg Ala Leu Phe Ser Leu Arg Asn Leu Lys Gly Pro Gly Pro Arg Asn 
        35                  40                  45              


Ala Leu Ile Leu Ala Ser Arg Asp Ser Ser Asn Leu Leu Ala His Glu 
    50                  55                  60                  


Ala Ala Phe Ala Leu Gly Gln Met Gln Asp Ala Glu Ala Ile Pro Ala 
65                  70                  75                  80  


Leu Glu Ser Val Leu Asn Asp Met Ser Leu His Pro Ile Val Arg His 
                85                  90                  95      


Glu Ala Ala Glu Ala Leu Gly Ala Ile Gly Leu Ala Gly Asn Val Asn 
            100                 105                 110         


Ile Leu Lys Lys Ser Leu Ser Ser Asp Pro Ala Gln Glu Val Arg Glu 
        115                 120                 125             


Thr Cys Glu Leu Ala Leu Lys Arg Ile Glu Asp Met Ser Asn Val Asp 
    130                 135                 140                 


Ala Glu Asn Gln Ser Ser Thr Thr Glu Lys Ser Pro Phe Met Ser Val 
145                 150                 155                 160 


Asp Pro Ala Gly Pro Ala Ala Ser Phe Ser Ser Val His Gln Leu Arg 
                165                 170                 175     


Gln Val Leu Leu Asp Glu Thr Lys Gly Met Tyr Glu Arg Tyr Ala Ala 
            180                 185                 190         


Leu Phe Ala Leu Arg Asn His Gly Gly Glu Glu Ala Val Ser Ala Ile 
        195                 200                 205             


Val Asp Ser Leu Ser Ala Ser Ser Ala Leu Leu Arg His Glu Val Ala 
    210                 215                 220                 


Tyr Val Leu Gly Gln Leu Gln Ser Lys Thr Ala Leu Ala Thr Leu Ser 
225                 230                 235                 240 


Lys Val Leu Arg Asp Val Asn Glu His Pro Met Val Arg His Glu Ala 
                245                 250                 255     


Ala Glu Ala Leu Gly Ser Ile Ala Asp Glu Gln Ser Ile Ala Leu Leu 
            260                 265                 270         


Glu Glu Phe Ser Lys Asp Pro Glu Pro Ile Val Ala Gln Ser Cys Glu 
        275                 280                 285             


Val Ala Leu Ser Met Leu Glu Phe Glu Asn Ser Gly Lys Ser Phe Glu 
    290                 295                 300                 


Phe Phe Phe Thr Gln Asp Pro Leu Val His 
305                 310                 


<210>  9
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  9
gtctacaggc atttgcaaga attactcg                                          28


<210>  10
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  10
ggatcctgtt ttatatttgt tgtaaaaagt ag                                     32


<210>  11
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  11
aagcttaggc atttgcaaga attactcg                                          28


<210>  12
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  12
atcgattgtt ttatatttgt tgtaaaaagt ag                                     32


<210>  13
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  13
ctcgagatgg gagaagctct gaagtacag                                         29


<210>  14
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  14
ggatcctcaa acttggcacc ttatctggg                                         29


<210>  15
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  15
ggatcctcag tactgacaat aaaaagattc ttg                                    33


<210>  16
<211>  35
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  16
atcgatatcg atactggatg gcggcgttag tatcg                                  35


<210>  17
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  17
atcgataaga attactcgtg agtaagg                                           27


<210>  18
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  18
gagctctttt tttttttttt tttt                                              24


