                         SEQUENCE LISTING

<110>  Helix Therapeutics LLC and Yale University
       DEL CAMPO, JACOB
       SCHLEIFMAN, ERICA
       BINDRA, RANJIT
       GLAZER, PETER M.
 
<120>  COMPOSITIONS AND METHODS FOR TARGETED INACTIVATION OF HIV CELL 
       SURFACE RECEPTORS

<130>  HT 101/YU 5346

<150>  U.S.S.N. 61/326,551
<151>  2010-04-21

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  1
tcgaaatgag aagaagagg                                                    19


<210>  2
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  2
cctcttcttc tcatttcga                                                    19


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic peptide nucleic acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  linked to Lys-Lys-Lys-JTJTTJTTJT-o-o-o- where Lys=lysine, J= 
       pseudoisocytosine, o- flexible 8-amino-3-6-dioxaoctanoic acid

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  linked to -Lys-Lys-Lys where Lys=lysine

<400>  3
tcttcttctc                                                              10


<210>  4
<211>  10
<212>  DNA
<213>  Homo sapiens

<400>  4
gagaagaaga                                                              10


<210>  5
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic peptide nucleic acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  linked to Lys-Lys-Lys-JTJTTJTTJT-OOO- where Lys=lysine,  J= 
       pseudoisocytosine, and o- flexible 8-amino-3-6-dioxaoctanoic acid

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  linked to -Lys-Lys-Lys, where Lys=lysine

<400>  5
tcttcttctc atttc                                                        15


<210>  6
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  6
gaaatgagaa gaaga                                                        15


<210>  7
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic peptide nucleic acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  linked to Lys-Lys-Lys-JTTJT-OOO-, where Lys=lysine, J= 
       pseudoisocytosine and o- flexible 8-amino-3-6-dioxaoctanoic acid

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  linked to -Lys-Lys-Lys, where Lys=lysine

<400>  7
tcttcttctc atttc                                                        15


<210>  8
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  8
tcttcttctc atttc                                                        15


<210>  9
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  9
tcctcgagaa gaaga                                                        15


<210>  10
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  10
ctgccgctgc ttgtcatggt cat                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence introduced by donor 591

<400>  11
ctgccctaag ctgtcatggt cat                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence introduced by donor 597

<400>  12
ctgccgctgc tctgaggggt cat                                               23


<210>  13
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic antisense oriented donor oligonucleotide targeting the 
       CCR5 gene

<400>  13
attcccgagt agcagatgac catgacagct tagggcagga ccagccccaa gatgactatc       60


<210>  14
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic antisense oriented donor oligonucleotide targeting the 
       CCR5 gene

<400>  14
tttaggattc ccgagtagca gatgacccct cagagcagcg gcaggaccag ccccaagatg       60


<210>  15
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic donor oligonucleotide designed to span the detlta32 
       deletion site

<400>  15
gatgactatc tttaatgtct ggaaattctt ccagaattaa ttaagactgt atggaaaatg       60

agagc                                                                   65


<210>  16
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic donor oligonucleotide designed to span the detlta32 
       deletion site

<400>  16
ccccaagatg actatcttta atgtctggaa cgatcatcag aattgatact gactgtatgg       60

aaaatg                                                                  66


<210>  17
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic donor oligonucleotide designed to span the detlta32 
       deletion site

<400>  17
gatgactatc tttaatgtct ggaaattcta ctagaattga tactgactgt atggaaaatg       60

agagc                                                                   65


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic allele-specific PCR forward primer "ASFUP"

<400>  18
gtcctgccgc tgctctgagg                                                   20


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic allele-specific PCR reverse primer "ASR1"

<400>  19
gtgtaaactg agcttgctcg ctc                                               23


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CCR5 gene-specific forward primer

<400>  20
acctttgggg tggtgacaag tgtg                                              24


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CCR5 gene-specific reverse primer

<400>  21
tctccccgac aaaggcatag atga                                              24


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic allele-specific PCR forward primer "JDCDELASF"

<400>  22
cattttccat acagtcttaa tt                                                22


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic allele-specific forward primer "JDCASF"

<400>  23
ccatacagtc agtatcaatt ctgatgatcg                                        30


<210>  24
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Restriction endonuclease selective PCR forward primer "RSB32PSPF"

<400>  24
gctctcattt tccatacagt cagtatcaat cc                                     32


<210>  25
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Restriction endonuclease selective PCR reverse primer "CCR5RSBR2"

<400>  25
gtagggagcc cagaagagaa aataaacaat cat                                    33


<210>  26
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic peptide nucleic acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  linked to JTJTTJTTJT-OOO-, where J=pseudoisocytosine, O= flexible
       8-amino-3,6-dioxaoctanoic acid, 6-aminohexanoic acid monomer

<400>  26
tcttcttctc                                                              10


<210>  27
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic peptide nucleic acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  linked to JTJTTJTTJT-OOO-, J=pseudoisocytosine, O= flexible 
       8-amino-3,6-dioxaoctanoic acid, 6-aminohexanoic acid monomer

<400>  27
tcttcttctc atttc                                                        15


<210>  28
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic peptide nucleic acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  linked to JTTJT-OOO-, where J=pseudoisocytosine, O= flexible 
       8-amino-3,6-dioxaoctanoic acid, 6-aminohexanoic acid monomer

<400>  28
tcttcttctc atttc                                                        15


