
                                SEQUENCE LISTING

<110> CENTOCOR ORTHO BIOTECH INC.

<120> Engineered Plant Cysteine Proteases and
  Their Uses

<130> CEN5283PCT

<140> To Be Assigned
<141> 2011-04-14

<150> 61/324972
<151> 2010-04-16  

<160> 68

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 233
<212> PRT
<213> Potato Virus

<220> 
<223> NIa protease

<400> 1
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 2
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> Potato virus C151S Mutant

<400> 2
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Ser Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 3
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> Potato Virus C110S Mutant

<400> 3
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 4
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> Potato Virus C181S Mutant

<400> 4
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  

Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 5
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> Potato Virus C211S Mutant

<400> 5
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     

Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 6
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> Potato Virus C19S/C110S/C181S Mutant

<400> 6
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Ser Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 7
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> Potato Virus C19S/C110S/C211S Mutant

<400> 7
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Ser Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 8
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA C19S/C181S/C211S mutant 

<400> 8
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Ser Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              

Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 9
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19S/C110S/C181S/C211S mutant

<400> 9
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Ser Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 

Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 10
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C110S/C181S mutant

<400> 10
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 11
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C110S/C211S mutant

<400> 11
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 12
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19A/C110S/C181S/C211S mutant

<400> 12
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      

Val Ile Ala Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 13
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C110S/C181S/C211S mutant

<400> 13
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         

Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 14
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19D/C110S/C181S/C211S mutant 

<400> 14
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Asp Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             

Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 15
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19E/C110S/C181S/C211S mutant

<400> 15
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Glu Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 16
<211> 235
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19F/C110S/C181S/C211S 

<400> 16
Met Gly Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile
 1               5                  10                  15      
Ser Ser Val Ile Phe Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr
            20                  25                  30          
Gln Leu Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His
        35                  40                  45              
Leu Phe Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly
    50                  55                  60                  
Val Phe Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu
65                  70                  75                  80  
Gly Arg Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe
                85                  90                  95      
Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser
            100                 105                 110         
Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser
        115                 120                 125             
Glu Thr Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His
    130                 135                 140                 
Trp Ile Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr
145                 150                 155                 160 
Thr Asp Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn
                165                 170                 175     
Thr Ser Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr
            180                 185                 190         
Leu Ala Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn
        195                 200                 205             
Ala Ser Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro
    210                 215                 220                 
Thr Lys Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230                 235 


<210> 17
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19G/C110S/C181S/C211S mutant

<400> 17
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Gly Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  

Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 18
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19H/C110S/C181S/C211S mutant

<400> 18
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile His Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     

Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 19
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19I/C110S/C181S/C211S mutant

<400> 19
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Ile Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 20
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19K/C110S/C181S/C211S mutant

<400> 20
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Lys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 21
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19L/C110S/C181S/C211S mutant

<400> 21
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Leu Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              

Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 22
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19M/C110S/C181S/C211S mutant

<400> 22
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Met Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 

Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 23
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19N/C110S/C181S/C211S mutant 

<400> 23
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Asn Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 24
<211> 156
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19P/C110S/C181S/C211S mutant

<400> 24
Glu Gly Arg Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro
 1               5                  10                  15      
Phe Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val
            20                  25                  30          
Ser Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val
        35                  40                  45              
Ser Glu Thr Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys
    50                  55                  60                  
His Trp Ile Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser
65                  70                  75                  80  
Thr Thr Asp Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr
                85                  90                  95      
Asn Thr Ser Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr
            100                 105                 110         
Tyr Leu Ala Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe
        115                 120                 125             
Asn Ala Ser Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys
    130                 135                 140                 
Pro Thr Lys Glu Phe Lys Thr Val Lys Leu Val Thr
145                 150                 155     


<210> 25
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19Q/C110S/C181S/C211S mutant

<400> 25
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Gln Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      

Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 26
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19R/C110S/C181S/C211S mutant

<400> 26
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Arg Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         

Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 27
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19T/C110S/C181S/C211S mutant

<400> 27
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Thr Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 28
<211> 156
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C181S/C211S mutant

<400> 28
Glu Gly Arg Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro
 1               5                  10                  15      
Phe Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val
            20                  25                  30          
Ser Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val
        35                  40                  45              
Ser Glu Thr Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys
    50                  55                  60                  
His Trp Ile Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser
65                  70                  75                  80  
Thr Thr Asp Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr
                85                  90                  95      
Asn Thr Ser Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr
            100                 105                 110         
Tyr Leu Ala Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe
        115                 120                 125             
Asn Ala Ser Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys
    130                 135                 140                 
Pro Thr Lys Glu Phe Lys Thr Val Lys Leu Val Thr
145                 150                 155     


<210> 29
<211> 156
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19W/C110S/C181S/C211S mutant

<400> 29
Glu Gly Arg Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro
 1               5                  10                  15      
Phe Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val
            20                  25                  30          
Ser Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val
        35                  40                  45              
Ser Glu Thr Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys
    50                  55                  60                  
His Trp Ile Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser
65                  70                  75                  80  
Thr Thr Asp Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr
                85                  90                  95      
Asn Thr Ser Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr
            100                 105                 110         
Tyr Leu Ala Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe
        115                 120                 125             
Asn Ala Ser Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys
    130                 135                 140                 

Pro Thr Lys Glu Phe Lys Thr Val Lys Leu Val Thr
145                 150                 155     


<210> 30
<211> 156
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19Y/C110S/C181S/C211S mutant

<400> 30
Glu Gly Arg Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro
 1               5                  10                  15      
Phe Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val
            20                  25                  30          
Ser Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val
        35                  40                  45              
Ser Glu Thr Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys
    50                  55                  60                  
His Trp Ile Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser
65                  70                  75                  80  
Thr Thr Asp Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr
                85                  90                  95      
Asn Thr Ser Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr
            100                 105                 110         
Tyr Leu Ala Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe
        115                 120                 125             
Asn Ala Ser Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys
    130                 135                 140                 
Pro Thr Lys Glu Phe Lys Thr Val Lys Leu Val Thr
145                 150                 155     


<210> 31
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C110S/C151S/C181S/C211S Mutant

<400> 31
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Ser Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 32
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C181S/C211S mutant

<400> 32
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     

Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 33
<211> 156
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C151S/C181S/C211S mutant

<400> 33
Glu Gly Arg Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro
 1               5                  10                  15      
Phe Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val
            20                  25                  30          
Ser Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val
        35                  40                  45              
Ser Glu Thr Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys
    50                  55                  60                  
His Trp Ile Ser Thr Lys Glu Gly His Ser Gly Ser Pro Ile Val Ser
65                  70                  75                  80  
Thr Thr Asp Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr
                85                  90                  95      
Asn Thr Ser Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr
            100                 105                 110         
Tyr Leu Ala Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe
        115                 120                 125             
Asn Ala Ser Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys
    130                 135                 140                 
Pro Thr Lys Glu Phe Lys Thr Val Lys Leu Val Thr
145                 150                 155     


<210> 34
<211> 233
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C151A mutant

<400> 34
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Ala Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr
225                 230             


<210> 35
<211> 241
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease with N-terminal 6x-His tag

<400> 35
Met Gly His His His His His His Ser Thr Ser Met Phe Arg Gly Val
 1               5                  10                  15      
Arg Asp Tyr Asn Pro Ile Ser Ser Val Ile Cys Gln Leu Glu Asn Glu
            20                  25                  30          
Ser Glu Gly Arg Thr Thr Gln Leu Phe Gly Leu Gly Phe Gly Pro Phe
        35                  40                  45              
Ile Ile Thr Asn Gln His Leu Phe Val Arg Asn Asn Gly Ser Leu Thr
    50                  55                  60                  
Val Arg Ser Gln Met Gly Val Phe Lys Val Asn Ser Thr Val Thr Leu
65                  70                  75                  80  
Gln Met Arg Pro Val Glu Gly Arg Asp Val Leu Ile Ile Lys Met Pro
                85                  90                  95      
Lys Asp Phe Pro Pro Phe Pro Gln Arg Leu Lys Phe Arg Gln Pro Thr
            100                 105                 110         
His Ser Glu Lys Val Cys Leu Ile Leu Thr Asn Phe Gln Gln Lys Ser
        115                 120                 125             

Ser Ser Ser Met Val Ser Glu Thr Ser His Ile Ile Pro Lys Glu Asn
    130                 135                 140                 
Thr Tyr Phe Trp Lys His Trp Ile Ser Thr Lys Glu Gly His Cys Gly
145                 150                 155                 160 
Ser Pro Ile Val Ser Thr Thr Asp Gly Ala Ile Leu Gly Ile His Ser
                165                 170                 175     
Leu Ser Asn Met Thr Asn Thr Ser Asn Tyr Phe Ala Cys Phe Pro Lys
            180                 185                 190         
Gly Phe Thr Glu Thr Tyr Leu Ala Thr Glu Ser Ala His Glu Trp Val
        195                 200                 205             
Lys Gly Trp Lys Phe Asn Ala Ser Asn Val Cys Trp Gly Ser Phe His
    210                 215                 220                 
Leu Gln Asp Ser Lys Pro Thr Lys Glu Phe Lys Thr Val Lys Leu Val
225                 230                 235                 240 
Thr
    


<210> 36
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease with C-terminal 6x-His tag

<400> 36
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             

Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr His His His His His His
225                 230                 235                 


<210> 37
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA C151S Protease with C-terminal 6x-His tag

<400> 37
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Cys Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Cys Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Ser Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Cys Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Cys Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr His His His His His His
225                 230                 235                 


<210> 38
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C181S/C211S with C-terminal 6x-His tag

<400> 38
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Val Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Cys Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr His His His His His His
225                 230                 235                 


<210> 39
<211> 239
<212> PRT
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C151S/C181S/C211S with C-terminal 6x-His tag

<400> 39
Ser Thr Ser Met Phe Arg Gly Val Arg Asp Tyr Asn Pro Ile Ser Ser
 1               5                  10                  15      
Val Ile Val Gln Leu Glu Asn Glu Ser Glu Gly Arg Thr Thr Gln Leu
            20                  25                  30          
Phe Gly Leu Gly Phe Gly Pro Phe Ile Ile Thr Asn Gln His Leu Phe
        35                  40                  45              
Val Arg Asn Asn Gly Ser Leu Thr Val Arg Ser Gln Met Gly Val Phe
    50                  55                  60                  
Lys Val Asn Ser Thr Val Thr Leu Gln Met Arg Pro Val Glu Gly Arg
65                  70                  75                  80  
Asp Val Leu Ile Ile Lys Met Pro Lys Asp Phe Pro Pro Phe Pro Gln
                85                  90                  95      
Arg Leu Lys Phe Arg Gln Pro Thr His Ser Glu Lys Val Ser Leu Ile
            100                 105                 110         
Leu Thr Asn Phe Gln Gln Lys Ser Ser Ser Ser Met Val Ser Glu Thr
        115                 120                 125             
Ser His Ile Ile Pro Lys Glu Asn Thr Tyr Phe Trp Lys His Trp Ile
    130                 135                 140                 
Ser Thr Lys Glu Gly His Ser Gly Ser Pro Ile Val Ser Thr Thr Asp
145                 150                 155                 160 
Gly Ala Ile Leu Gly Ile His Ser Leu Ser Asn Met Thr Asn Thr Ser
                165                 170                 175     
Asn Tyr Phe Ala Ser Phe Pro Lys Gly Phe Thr Glu Thr Tyr Leu Ala
            180                 185                 190         
Thr Glu Ser Ala His Glu Trp Val Lys Gly Trp Lys Phe Asn Ala Ser
        195                 200                 205             
Asn Val Ser Trp Gly Ser Phe His Leu Gln Asp Ser Lys Pro Thr Lys
    210                 215                 220                 
Glu Phe Lys Thr Val Lys Leu Val Thr His His His His His His
225                 230                 235                 

<210> 40
<211> 699
<212> DNA
<213> Potato Virus

<220> 
<223> NIa protease

<400> 40
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatctgccag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagtttgc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac tgcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
tgcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt tgctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttacc                        699

<210> 41
<211> 699
<212> DNA
<213> Artificial Sequence

<220> 
<223> PVA C151S mutant 

<400> 41
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatctgccag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagtttgc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac agcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
tgcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt tgctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttacc                        699

<210> 42
<211> 699
<212> DNA
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C181S/C211S mutant 

<400> 42
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatcgttcag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagttagc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac tgcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
agcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt agctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttacc                        699

<210> 43
<211> 699
<212> DNA
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C151S/C181S/C211S mutant 

<400> 43
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatcgttcag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagttagc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac agcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
agcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt agctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttacc                        699

<210> 44
<211> 723
<212> DNA
<213> Artificial Sequence

<220> 
<223> PVA Protease with N-terminal 6x-His tag

<400> 44
atgggccacc atcaccatca ccattctacc tctatgttcc gtggtgttcg tgactacaac 60
ccgatctctt ctgttatctg ccagctggaa aacgaatctg aaggtcgtac cacccagctg 120
ttcggtctgg gtttcggtcc gttcatcatc accaaccagc acctgttcgt tcgtaacaac 180
ggttctctga ccgttcgttc tcagatgggt gttttcaaag ttaactctac cgttaccctg 240
cagatgcgtc cggttgaagg tcgtgacgtt ctgatcatca aaatgccgaa agacttcccg 300
ccgttcccgc agcgtctgaa attccgtcag ccgacccact ctgaaaaagt ttgcctgatc 360
ctgaccaact tccagcagaa atcttcttct tctatggttt ctgaaacctc tcacatcatc 420
ccgaaagaaa acacctactt ctggaaacac tggatctcta ccaaagaagg tcactgcggt 480
tctccgatcg tttctaccac cgacggtgcg atcctgggta tccactctct gtctaacatg 540
accaacacct ctaactactt cgcgtgcttc ccgaaaggtt tcaccgaaac ctacctggcg 600
accgaatctg cgcacgaatg ggttaaaggt tggaaattca acgcgtctaa cgtttgctgg 660
ggttctttcc acctgcagga ctctaaaccg accaaagaat tcaaaaccgt taaactggtt 720
acc                                                               723

<210> 45
<211> 717
<212> DNA
<213> PVA Protease with C-terminal 6x-His tag

<220> 
<223> To Be Entered

<400> 45
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatctgccag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagtttgc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac tgcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
tgcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt tgctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttaccc accatcacca tcaccat    717

<210> 46
<211> 717
<212> DNA
<213> Artificial Sequence

<220> 
<223> PVA C151S mutant with C-terminal 6x-His tag

<400> 46
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatctgccag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagtttgc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac agcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
tgcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt tgctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttaccc accatcacca tcaccat    717

<210> 47
<211> 723
<212> DNA
<213> PVA Protease C19V/C110S/C181S/C211S mutant with C-terminal 6x-His tag

<220> 
<223> To Be Entered

<400> 47
atgggctcta cctctatgtt ccgtggtgtt cgtgactaca acccgatctc ttctgttatc 60
gttcagctgg aaaacgaatc tgaaggtcgt accacccagc tgttcggtct gggtttcggt 120
ccgttcatca tcaccaacca gcacctgttc gttcgtaaca acggttctct gaccgttcgt 180
tctcagatgg gtgttttcaa agttaactct accgttaccc tgcagatgcg tccggttgaa 240
ggtcgtgacg ttctgatcat caaaatgccg aaagacttcc cgccgttccc gcagcgtctg 300
aaattccgtc agccgaccca ctctgaaaaa gttagcctga tcctgaccaa cttccagcag 360
aaatcttctt cttctatggt ttctgaaacc tctcacatca tcccgaaaga aaacacctac 420
ttctggaaac actggatctc taccaaagaa ggtcactgcg gttctccgat cgtttctacc 480
accgacggtg cgatcctggg tatccactct ctgtctaaca tgaccaacac ctctaactac 540
ttcgcgagct tcccgaaagg tttcaccgaa acctacctgg cgaccgaatc tgcgcacgaa 600
tgggttaaag gttggaaatt caacgcgtct aacgttagct ggggttcttt ccacctgcag 660
gactctaaac cgaccaaaga attcaaaacc gttaaactgg ttacccacca tcaccatcac 720
cat                                                               723

<210> 48
<211> 717
<212> DNA
<213> Artificial Sequence

<220> 
<223> PVA Protease C19V/C110S/C151S/C181S/C211S mutant with C-terminal 6x-His tag

<400> 48
tctacctcta tgttccgtgg tgttcgtgac tacaacccga tctcttctgt tatcgttcag 60
ctggaaaacg aatctgaagg tcgtaccacc cagctgttcg gtctgggttt cggtccgttc 120
atcatcacca accagcacct gttcgttcgt aacaacggtt ctctgaccgt tcgttctcag 180
atgggtgttt tcaaagttaa ctctaccgtt accctgcaga tgcgtccggt tgaaggtcgt 240
gacgttctga tcatcaaaat gccgaaagac ttcccgccgt tcccgcagcg tctgaaattc 300
cgtcagccga cccactctga aaaagttagc ctgatcctga ccaacttcca gcagaaatct 360
tcttcttcta tggtttctga aacctctcac atcatcccga aagaaaacac ctacttctgg 420
aaacactgga tctctaccaa agaaggtcac agcggttctc cgatcgtttc taccaccgac 480
ggtgcgatcc tgggtatcca ctctctgtct aacatgacca acacctctaa ctacttcgcg 540
agcttcccga aaggtttcac cgaaacctac ctggcgaccg aatctgcgca cgaatgggtt 600
aaaggttgga aattcaacgc gtctaacgtt agctggggtt ctttccacct gcaggactct 660
aaaccgacca aagaattcaa aaccgttaaa ctggttaccc accatcacca tcaccat    717

<210> 49
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 49
ctaaccatgg gctctacctc tatgttccgt ggtgttcgtg actacaa               47

<210> 50
<211> 51
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 50
gttactcgag ttattaatgg tgatggtgat ggtgggtaac cagtttaacg g          51

<210> 51
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 51
ctaccaaaga cggtcagagc ggttctccga tcgtttc                          37

<210> 52
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 52
gaaacgatcg gagaaccgct ctgaccgtct ttggtag                          37

<210> 53
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 53
ctctaccaaa gaaggtcacg ccggttctcc gatcgtttc                        39

<210> 54
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 54
gaaacgatcg gagaaccggc gtgaccttct ttggtagag                        39

<210> 55
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 55
cccgatctct tctgttatca gccagctgga aaacgaatct gaagg                 45

<210> 56
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 56
ccttcagatt cgttttccag ctggctgata acagaagaga tcggg                 45

<210> 57
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 57
cgacccactc tgaaaaagtt agcctgatcc tgaccaactt ccag                  44

<210> 58
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 58
ctggaagttg gtcaggatca ggctaacttt ttcagagtgg gtcg                  44

<210> 59
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 59
cacctctaac tacttcgcga gcttcccgaa aggtttcacc g                     41

<210> 60
<211> 41
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 60
cggtgaaacc tttcgggaag ctcgcgaagt agttagaggt g                     41

<210> 61
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 61
caacgcgtct aacgttagct ggggttcttt ccacctg                          37

<210> 62
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 62
caggtggaaa gaaccccagc taacgttaga cgcgttg                          37

<210> 63
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 63
acccgatctc ttctgttatc nnkcagctgg aaaacgaatc tgaag                 45

<210> 64
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> mutagenesis primer

<400> 64
cttcagattc gttttccagc tgmnngataa cagaagagat cgggt                 45

<210> 65
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Consensus NIa cleavage site, has N-glycosylation site

<400> 65
Glu Asn Val Thr Phe Gln Gly
 1               5          


<210> 66
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Consensus NIa cleavage site without N-glycosylation site

<400> 66
Glu Asn Val Thr Phe Gln Gly
 1               5          


<210> 67
<211> 145
<212> PRT
<213> Artificial Sequence

<220> 
<223> NIa protease fusion substrate with ENVTFQG recognition site

<400> 67
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro
 1               5                  10                  15      
Arg Gly Ser His Met Thr Thr Glu Asn Val Thr Phe Gln Gly Ser Thr
            20                  25                  30          
Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His
        35                  40                  45              
Met Asp Ser Pro Asp Leu Gly Thr Leu Pro Ala Pro Lys Asn Leu Val
    50                  55                  60                  
Val Ser Glu Val Thr Glu Asp Ser Leu Arg Leu Ser Trp Thr Ala Pro
65                  70                  75                  80  
Asp Ala Ala Phe Asp Ser Phe Leu Ile Gln Tyr Gln Glu Ser Glu Lys
                85                  90                  95      
Val Gly Glu Ala Ile Asn Leu Thr Val Pro Gly Ser Glu Arg Ser Tyr
            100                 105                 110         
Asp Leu Thr Gly Leu Lys Pro Gly Thr Glu Tyr Thr Val Ser Ile Tyr
        115                 120                 125             
Gly Val Lys Gly Gly His Arg Ser Asn Pro Leu Ser Ala Glu Phe Thr
    130                 135                 140                 
Thr
145 


<210> 68
<211> 146
<212> PRT
<213> Artificial Sequence

<220> 
<223> NIa protease fusion substrate with EAVTFQG recognition site

<400> 68
Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro
 1               5                  10                  15      
Arg Gly Ser His Met Thr Thr Glu Ala Val Thr Phe Gln Gly Ser Thr
            20                  25                  30          
Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His
        35                  40                  45              
Met Asp Ser Pro Asp Leu Gly Thr Met Leu Pro Ala Pro Lys Asn Leu
    50                  55                  60                  
Val Val Ser Glu Val Thr Glu Asp Ser Leu Arg Leu Ser Trp Thr Ala
65                  70                  75                  80  
Pro Asp Ala Ala Phe Asp Ser Phe Leu Ile Gln Tyr Gln Glu Ser Glu
                85                  90                  95      
Lys Val Gly Glu Ala Ile Asn Leu Thr Val Pro Gly Ser Glu Arg Ser
            100                 105                 110         
Tyr Asp Leu Thr Gly Leu Lys Pro Gly Thr Glu Tyr Thr Val Ser Ile
        115                 120                 125             
Tyr Gly Val Lys Gly Gly His Arg Ser Asn Pro Leu Ser Ala Glu Phe
    130                 135                 140                 
Thr Thr
145     

