                         SEQUENCE LISTING

<110>  Georgia Tech Research Corporation
       Gaucher, Eric
 
<120>  VARIANTS OF ANCESTRAL URICASES AND USES THEREOF

<130>  9844-11WO

<150>  US 61/322,094
<151>  2010-04-08

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  1

Met Ala His Tyr His Gly His Leu Thr Lys Asn Ala Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Val Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys His His Ile Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Asn Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Thr Ile Glu Ala Phe Ala Met Asn Ile Gly Lys 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Val Pro Trp Lys Arg Phe Glu Lys Asn Gly Val Asn His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Lys Arg Gly Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Arg Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr Asp Gln Ser Arg Ala Val Asp Phe Glu 
        195                 200                 205             


Ala Ile Trp Asp Thr Val Leu Asp Ile Val Leu Glu Lys Phe Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  2
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  2

Met Ala His Tyr His Asn Asp Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Ala Phe Ala Met Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Val Pro Trp Lys Arg Phe Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Met Arg Ser Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Gly Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Asp Thr Val Arg Asp Ile Val Leu Glu Lys Phe Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  3
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  3

Met Ala His Tyr His Asn Asp Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Ala Phe Ala Val Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys Arg Leu Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Asp Thr Ile Arg Asp Leu Val Leu Glu Lys Phe Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  4
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  4

Met Ala His Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Ala Phe Gly Val Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys Arg Leu Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Asp Thr Ile Arg Asp Leu Val Leu Glu Lys Phe Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  5
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  5

Met Ala His Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Ala Phe Gly Val Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys Arg Leu Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Asp Thr Ile Arg Asp Leu Val Leu Glu Lys Ser Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  6
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  6

Met Ala His Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Ala Phe Gly Val Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys His Leu Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Asp Thr Ile Arg Asp Leu Val Leu Glu Lys Ser Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  7
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  7

Met Ala His Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Glu Ile Lys Ser Ile Glu Ala Phe Gly Val Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys His Leu Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Gln Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Lys 
        195                 200                 205             


Ala Thr Trp Asp Thr Ile Arg Asp Leu Val Met Glu Lys Ser Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Asp Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Cys 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Ala Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  8
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is Asn or Gly

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Asp, Asn or His

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is Tyr or Leu

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is Lys or Thr

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa is Asp or Ala

<220>
<221>  MISC_FEATURE
<222>  (24)..(24)
<223>  Xaa is Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (25)..(25)
<223>  Xaa is Met or Val

<220>
<221>  MISC_FEATURE
<222>  (37)..(37)
<223>  Xaa is Tyr or His

<220>
<221>  MISC_FEATURE
<222>  (39)..(39)
<223>  Xaa is Ser or Ile

<220>
<221>  MISC_FEATURE
<222>  (52)..(52)
<223>  Xaa is Ser or Asn

<220>
<221>  MISC_FEATURE
<222>  (83)..(83)
<223>  Xaa is Gly or Glu

<220>
<221>  MISC_FEATURE
<222>  (86)..(86)
<223>  Xaa is Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (91)..(91)
<223>  Xaa is Ala or Gly

<220>
<221>  MISC_FEATURE
<222>  (92)..(92)
<223>  Xaa is Met or Val

<220>
<221>  MISC_FEATURE
<222>  (95)..(95)
<223>  Xaa is Cys or Gly

<220>
<221>  MISC_FEATURE
<222>  (96)..(96)
<223>  Xaa is Glu or Lys

<220>
<221>  MISC_FEATURE
<222>  (103)..(103)
<223>  Xaa is Asn or Lys

<220>
<221>  MISC_FEATURE
<222>  (112)..(112)
<223>  Xaa is Val or Met

<220>
<221>  MISC_FEATURE
<222>  (115)..(115)
<223>  Xaa is Val or Ile

<220>
<221>  MISC_FEATURE
<222>  (119)..(119)
<223>  Xaa is Arg or His

<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  Xaa is Phe or Leu

<220>
<221>  MISC_FEATURE
<222>  (121)..(121)
<223>  Xaa is Glu or Gly

<220>
<221>  MISC_FEATURE
<222>  (126)..(126)
<223>  Xaa is Lys or Asn

<220>
<221>  MISC_FEATURE
<222>  (146)..(146)
<223>  Xaa is Met, Leu or Lys

<220>
<221>  MISC_FEATURE
<222>  (147)..(147)
<223>  Xaa is Arg or Lys

<220>
<221>  MISC_FEATURE
<222>  (148)..(148)
<223>  Xaa is Ser or Gly

<220>
<221>  MISC_FEATURE
<222>  (151)..(151)
<223>  Xaa is Pro or Gln

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is Gln, Arg or Glu

<220>
<221>  MISC_FEATURE
<222>  (178)..(178)
<223>  Xaa is Phe or Tyr

<220>
<221>  MISC_FEATURE
<222>  (192)..(192)
<223>  Xaa is Gln or Lys

<220>
<221>  MISC_FEATURE
<222>  (200)..(200)
<223>  Xaa is His or Asp

<220>
<221>  MISC_FEATURE
<222>  (202)..(202)
<223>  Xaa is Gly, Cys or Ser

<220>
<221>  MISC_FEATURE
<222>  (204)..(204)
<223>  Xaa is Asp or Ala

<220>
<221>  MISC_FEATURE
<222>  (208)..(208)
<223>  Xaa is Glu or Lys

<220>
<221>  MISC_FEATURE
<222>  (210)..(210)
<223>  Xaa is Thr or Ile

<220>
<221>  MISC_FEATURE
<222>  (214)..(214)
<223>  Xaa is Val or Ile

<220>
<221>  MISC_FEATURE
<222>  (215)..(215)
<223>  Xaa is Arg or Leu

<220>
<221>  MISC_FEATURE
<222>  (217)..(217)
<223>  Xaa is Ile or Leu

<220>
<221>  MISC_FEATURE
<222>  (219)..(219)
<223>  Xaa is Leu or Met

<220>
<221>  MISC_FEATURE
<222>  (220)..(220)
<223>  Xaa is Glu or Lys

<220>
<221>  MISC_FEATURE
<222>  (222)..(222)
<223>  Xaa is Phe or Ser

<220>
<221>  MISC_FEATURE
<222>  (229)..(229)
<223>  Xaa is Gly or Asp

<220>
<221>  MISC_FEATURE
<222>  (230)..(230)
<223>  Xaa is Glu or Lys

<220>
<221>  MISC_FEATURE
<222>  (232)..(232)
<223>  Xaa is Ser or Leu

<220>
<221>  MISC_FEATURE
<222>  (233)..(233)
<223>  Xaa is Pro or Thr

<220>
<221>  MISC_FEATURE
<222>  (240)..(240)
<223>  Xaa is Tyr or Cys

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is Glu or Ala

<220>
<221>  MISC_FEATURE
<222>  (265)..(265)
<223>  Xaa is Tyr or Leu

<220>
<221>  MISC_FEATURE
<222>  (273)..(273)
<223>  Xaa is Met or Gly

<220>
<221>  MISC_FEATURE
<222>  (286)..(286)
<223>  Xaa is Asp, Ser or Cys

<220>
<221>  MISC_FEATURE
<222>  (303)..(303)
<223>  Xaa is Arg or Lys

<400>  8

Met Ala His Tyr His Xaa Xaa Xaa Xaa Lys Asn Xaa Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Xaa Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Xaa His Xaa Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Xaa Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Xaa Ile Lys Xaa Ile Glu Ala Phe Xaa Xaa Asn Ile Xaa Xaa 
                85                  90                  95      


His Phe Leu Ser Ser Phe Xaa His Val Ile Arg Ala Gln Val Tyr Xaa 
            100                 105                 110         


Glu Glu Xaa Pro Trp Lys Xaa Xaa Xaa Lys Asn Gly Val Xaa His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Xaa Xaa Xaa Gly Pro Xaa Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Xaa Asp 
                165                 170                 175     


Xaa Xaa Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Xaa 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr Xaa Gln Xaa Arg Xaa Val Asp Phe Xaa 
        195                 200                 205             


Ala Xaa Trp Asp Thr Xaa Xaa Asp Xaa Val Xaa Xaa Lys Xaa Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Xaa Xaa Tyr Xaa Xaa Ser Val Gln Lys Thr Leu Xaa 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Xaa Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Xaa Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Xaa Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Xaa Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Xaa Leu 
    290                 295                 300                 


<210>  9
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic uricase sequence

<400>  9

Met Ala His Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Glu Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Glu Ile Lys Ser Ile Glu Ala Phe Gly Val Asn Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Met 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys His Leu Gly Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Gln Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Lys 
        195                 200                 205             


Ala Thr Trp Asp Thr Ile Arg Asp Leu Val Met Glu Lys Ser Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Leu Thr Ser Val Gln Lys Thr Leu Cys 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Ala Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


<210>  10
<211>  304
<212>  PRT
<213>  Sus scrofa

<400>  10

Met Ala His Tyr Arg Asn Asp Tyr Lys Lys Asn Asp Glu Val Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Ile Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Val Ile Pro Thr Asp Thr Ile Lys Asn Thr Val Asn Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Thr Phe Ala Val Thr Ile Cys Glu 
                85                  90                  95      


His Phe Leu Ser Ser Phe Lys His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Val Pro Trp Lys Arg Phe Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile Tyr Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Ile Arg Asn Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Gly Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Asp Thr Val Arg Ser Ile Val Leu Gln Lys Phe Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Thr Leu Gly Gln Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Leu Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Arg Ile Thr Gly Thr Val Lys Arg Lys Leu Thr Ser Arg Leu 
    290                 295                 300                 


<210>  11
<211>  304
<212>  PRT
<213>  Papio hamadryas

<400>  11

Met Ala Asp Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Leu Glu Phe 
1               5                   10                  15      


Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His Ile Gln 
            20                  25                  30          


Arg Asp Gly Lys Tyr His Ser Ile Lys Glu Val Ala Thr Ser Val Gln 
        35                  40                  45              


Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 
    50                  55                  60                  


Ile Ile Pro Thr Asp Thr Ile Lys Asn Thr Val His Val Leu Ala Lys 
65                  70                  75                  80  


Phe Lys Gly Ile Lys Ser Ile Glu Ala Phe Gly Val Asn Ile Cys Glu 
                85                  90                  95      


Tyr Phe Leu Ser Ser Phe Asn His Val Ile Arg Ala Gln Val Tyr Val 
            100                 105                 110         


Glu Glu Ile Pro Trp Lys Arg Leu Glu Lys Asn Gly Val Lys His Val 
        115                 120                 125             


His Ala Phe Ile His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 
    130                 135                 140                 


Gln Leu Arg Ser Gly Pro Pro Val Ile His Ser Gly Ile Lys Asp Leu 
145                 150                 155                 160 


Lys Val Leu Lys Thr Thr Gln Ser Gly Phe Glu Gly Phe Ile Lys Asp 
                165                 170                 175     


Gln Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gln 
            180                 185                 190         


Val Tyr Cys Lys Trp Arg Tyr His Gln Cys Arg Asp Val Asp Phe Glu 
        195                 200                 205             


Ala Thr Trp Gly Thr Ile Arg Asp Leu Val Leu Glu Lys Phe Ala Gly 
    210                 215                 220                 


Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gln Lys Thr Leu Tyr 
225                 230                 235                 240 


Asp Ile Gln Val Leu Ser Leu Ser Arg Val Pro Glu Ile Glu Asp Met 
                245                 250                 255     


Glu Ile Ser Leu Pro Asn Ile His Tyr Phe Asn Ile Asp Met Ser Lys 
            260                 265                 270         


Met Gly Leu Ile Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 
        275                 280                 285             


Tyr Gly Lys Ile Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 
    290                 295                 300                 


