                         SEQUENCE LISTING

<110>  CONSORCIO DE TECNOLOGIA E INNOVACION EN SALUD S.A.
 
<120>  SYNTHETIC CONSTRUCTS FOR POLYNUCLEOTIDE AND PROTEIN EXPRESSION

<130>  P-S-8113-113

<150>  US61322425
<151>  2010-04-09

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  Multimer of Tcf sites of the COX2 promoter

<400>  1
ggtaccctta cccgctacaa agattacccg ctacaaagat tacccgctac aaagattacc       60

cgctacaaag attaccccct cgag                                              84


<210>  2
<211>  211
<212>  DNA
<213>  Rattus rattus

<400>  2
ctcgaggtct ctagggccag ccagtgctcc agctgaggct gagagagatg gcacacagta       60

ggggtgctgg agcagcccct ccgggaagag gtctggggcc atgtcaggac ccggcagcct      120

ctgattgtgt cctaccctcc ccttccaggc cttcgccccg gcagctgcag tcaccaacca      180

cagcatcctt tgggtttgac ctattgagct c                                     211


<210>  3
<211>  655
<212>  DNA
<213>  Homo sapiens

<400>  3
gagctcttcc ttaggcatca gctctctaca attctcacat tgagaatatg tgtattttgt       60

tagctcaaac cttgttagac atgttaaatg tttagaaata taaatttaac ctaccccttg      120

aggtaggtct tgagaggttt gtgagcctaa aaagacatgg aggaaccact tattgccaca      180

agcacattgt tctaaattat ttggaatcag ttaattcttc cccatctcct acccatgcct      240

gacaccaaag aggagcctct aaatttacag ggaatacaag gaagtctact gttctctgct      300

cctctctggg ttattagggc acatgggagc cctcagttgt tttctgctga gcaagagcaa      360

agtccacctt ggacttagac agcttgccaa attttttgcc agaaggggac ctgagttgtg      420

accactccca gtgtgtgccg ggaaaaggct cgtactggtg ccagaatctc ttactgtcaa      480

tgctcccaaa actcaccgct tgcccccacc ccttttgctt aaatgacgtg gttcttatct      540

cagatcctga tataaagctc ctacagctac ctggcctgag aagccaactc agactcagcc      600

aacaggtaag tgggcattac aggagaaggg cgtctctaac atgcactgta gatct           655


<210>  4
<211>  289
<212>  DNA
<213>  Artificial

<220>
<223>  Multimer of Tcf sites of the COX2 promoter and fragment 
       comprising between the -287 and -105 base pairs of the nucleosome
       positioning region of the rat osteocalcin gene promoter

<400>  4
ggtaccctta cccgctacaa agattacccg ctacaaagat tacccgctac aaagattacc       60

cgctacaaag attaccccct cgaggtctct agggccagcc agtgctccag ctgaggctga      120

gagagatggc acacagtagg ggtgctggag cagcccctcc gggaagaggt ctggggccat      180

gtcaggaccc ggcagcctct gattgtgtcc taccctcccc ttccaggcct tcgccccggc      240

agctgcagtc accaaccaca gcatcctttg ggtttgacct attgagctc                  289


<210>  5
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer sequence for rat osteocalcin promoter

<400>  5
actcgaggtc tctagggcca gccagt                                            26


<210>  6
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer sequence for rat osteocalcin promoter

<400>  6
cgagctcagg agatgctgcc aggacta                                           27


<210>  7
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer sequence for human REG1A promoter

<400>  7
accatctcga gagtttatca aatagcttat aacttc                                 36


<210>  8
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer sequence for human REG1A promoter

<400>  8
tgtatcttcc cgaagatttt agatctacag tgcat                                  35


<210>  9
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  9
ctagcaaaat aggctgtc                                                     18


<210>  10
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  10
cgccgggcct ttctttatg                                                    19


<210>  11
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Canonical Tcf binding site


<400>  11
tcaaag                                                                  6


<210>  12
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Canonical Tcf binding site


<400>  12
acaaag                                                                  6


<210>  13
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Consensus sequence of the COX2 gene promoter


<400>  13
caaag                                                                   5


